Abstract: A reduced graphene oxide-based biosensor includes a nano-structure field-effect transistor including a channel region which includes a reduced graphene oxide having a linking moiety to be bonded to a receptor specific to an analyte, and which is represented by a formula of —(C?O)—X—COOH, wherein X represents a C1-C3 alkenylene group or a C1-C3 alkylene group.

Abstract: The present invention relates to a method for screening a sol composition for sol-gel biochips, which is used to immobilize a probe on a surface-untreated substrate, also relates to a sol composition screened by said method and a sol-gel biochip comprising said sol composition immobilized thereon. The sol composition screened by the disclosed method can be mixed with a probe, and the sol mixture can be integrated on 96-well plates, which are widely used in existing bioassays, without surface treatment. Also, the biochip can provide a sensitive and specific good analysis results because this immobilization methods of probe maintain the nature of probes without modification.

Abstract: The present invention provides methods for predicting post-operative recurrence of Crohn's disease (CD) in a subject. With the present invention it is possible to predict whether a patient undergoing surgical treatment of CD is at risk of developing histological, radiographic, endoscopic, and/or clinical recurrence of the disease.

Abstract: The present invention provides a method for analyzing levels of markers of subpopulations of vesicles in a sample comprising a heterogeneous population of said vesicles, the method comprising a) contacting said sample with a set of at least two capture agents, marker of said vesicles; thereby capturing at least two subpopulations of said vesicles, b) contacting the captured at least two subpopulations of vesicles with at least two different detection agents, thereby labeling at least two markers of said at least two subpopulations of said vesicles inversely with said at least two capture agents and at least two detection agents, thereby generating an at least 2×2 matrix of signal intensities, and c) determining the levels of said at least two markers of said at least two subpopulations of said vesicles, wherein said levels of said at least two markers of subpopulations of said vesicles are determined relative to each other.

Abstract: The present invention provides systems, devices, and methods for point-of-care and/or distributed testing services. The methods and devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device can be modified to allow for more flexible and robust use with the disclosed methods for a variety of medical, laboratory, and other applications. The systems, devices, and methods of the present invention can allow for effective use of samples by improved sample preparation and analysis.

Abstract: Value-assigned solutions having predetermined concentrations of recombinant Lp-PLA2 are described herein. In particular, described herein are solutions of rLp-PLA2 that are stable for an extended period of time. Kits and assays include these calibration solutions, as well as methods of making and using them are described.

Abstract: The present invention primarily relates to a method for stratification of a patient for assessing the suitability of a therapy. The invention also relates to a method for the prognosis of the outcome of a disease of a patient and a method for the prediction and/or detection of therapy resistance of a patient towards a therapy. Furthermore, the invention relates to a novel kit, and corresponding uses thereof.

Abstract: Methods and kits are provided for determining lung cancer disease progression or for pathological stratification of solitary indeterminate nodules detected during lung cancer screening or for determining the likelihood of disease progression in a subject. The method includes obtaining a biological sample from the subject and assaying a level in the biological sample of a biomarker in a panel of biomarkers where the panel includes at least one biomarker. The method further includes comparing the biomarker level in the subject's sample to a cutoff value for each biomarker measured for the panel of biomarkers and determining whether the biomarker level is above or below the cutoff value.

Abstract: The present invention is related to the anti-Uroplakin II antibodies, kits, cocktails, and use of anti-Uroplakin II antibodies for detection of cancer.

Abstract: The current disclosure provides methods for detecting and quantitating the 6-O-methylguanine-DNA methyltransferase protein (MGMT) directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring/Multiple Reaction Monitoring (SRM/MRM) mass spectrometry. Such biological samples are chemically preserved and fixed with formaldehyde containing agents/fixatives and may include formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and/or paraffin embedded. A protein sample is prepared from the biological sample and the MGMT protein is quantitated in the sample using SRM/MRM mass spectrometry by quantitating one or more fragment peptides.

Abstract: We describe a method of monitoring the state of a cell, tissue, organ or organism. The method comprises establishing, for a sample of micro-particles from the cell, tissue, organ or organism, a ratio. The ratio is of a selected polypeptide in microparticles which comprise GM1 gangliosides, preferably which bind to Cholera Toxin B (CTB) (“GM1 ganglioside microparticle polypeptide”) to the selected polypeptide in microparticles which comprise exposed phosphotidylserine, preferably which bind to Annexin V (“Annexin V microparticle polypeptide”). The GM1 ganglioside microparticle polypeptide to Annexin V microparticle polypeptide ratio so established may be indicative of the state of the cell, tissue, organ or organism.

Abstract: One or more cells are labeled with minor stable isotopes, characterized, and preserved for subsequent use as a bio-specimen reference standard. The one or more cells are grown in culture media supplied with minor stable isotopes in concentrations substantially different from normally occurring concentrations, thereby supplanting major stable isotopes that would normally be incorporated into the proteins of the cells. The proteins of the cells are thus labeled by the minor stable isotopes and can be used in protcomic characterization of the cells. The cells are preserved by fixation as a reference standard. Cells of the reference standard are mixed with the sample and subject to mass spectrometry evaluation, whereby the labeled proteins of the reference standard can be used in determining the proteome of the sample.

Abstract: Methods are provided for detecting and quantifying the Mesothelin protein (MSLN) in biological samples that have been fixed in formalin using Selected Reaction Monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry. The biological sample may be, for example, tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from the biological sample and the MSLN protein is quantitated by SRM/MRM mass spectrometry by quantitating one or more MSLN fragment peptides in the protein sample.

Abstract: The present invention relates to a method which may be used for the analysis of endogenously formed antibodies such as anti-drug antibodies (ADAs) or rheumatoid factor (RF) in a solution, which method comprises (a) contacting the solution with a solid phase to which e.g. IgG molecule(s) have been attached; (b) allowing the ADAs or RF to bind specifically to the attached molecule(s); (c) adding a labeled isotype-specific reagent capable of binding ADAs or RF; (d) removing any excess of reagent; and (f) detecting the label bound or unbound to determine directly or indirectly the presence or concentration of ADAs in the solution. The molecule(s) have been attached to the solid phase via a linker, which may be an organic molecule, an amino acid, a peptide, a protein a molecule of protein origin, a monosaccharide, an oligosaccharide or a polysaccharide. The method according to the invention may be used e.g. as an immunoassay for making clinical decisions in patient care, as well as in the development of new drugs.

Abstract: The present invention relates to the field of autism. More specifically, the present invention provides methods for treating individuals with autism spectrum disorders. The present invention also provides methods for predicting a likelihood of ASD. In one embodiment, a method for treating a female patient having a high risk factor of having children with ASD comprises the steps of (a) identifying in a sample taken from the female patient the presence of AT-1 antibodies; and (b) administering an effective amount of an AT-1 inhibitor or inhibitor of AT-1 antibodies to the female patient.

Abstract: The present invention pertains to the specific detection of MIF, in particular oxMIF, and of anti-oxMIF antibodies in tissues. A detection method is provided which uses immunohistochemistry or immunofluorescence and wherein specific anti-oxMIF antibodies and specific idiotypic monoclonal rabbit antibodies are used.

Abstract: The present invention encompasses methods of identifying proteins and protein modifications of proteins specifically associated with a chromatin.

Abstract: Provided is a method of bioassay for the quantification of peptide fragments elevated in lung diseases such as COPD, SCC, or IPF. The peptide fragments comprise a neo-epitope formed at a cleavage site by cleavage in vivo of elastin by a proteinase. In the method a sample is contacted with an antibody having specific binding affinity for the neo-epitope amino acid sequence and determining the level of binding is determined where the antibody binds one of the following terminal sequences: . . . FGPGVV, . . . VPGLGV or IKAPKL . . . . Also provided are antibodies and immunoassay kits for use in such methods.

Abstract: The invention relates to a method for prognosing, diagnosing, determining the risk, and monitoring the evolution of a muscular dystrophy. It also relates to a method for evaluating the efficacy of a treatment of a muscular dystrophy in a subject in need thereof.

Abstract: Disclosed is a method for diagnosing allergy in a human or animal patient, wherein the level of species-specific lipocalin, especially lipocalin 2 in a human patient, is measured in a sample of said patient and wherein a lowered level of said lipocalin compared to the level of said lipocalin in the corresponding sample of a human or animal that has no allergy, is indicative of an allergy, as well as a kit for performing this method, and a kit for quality control of allergen molecules or extracts used for immunotherapy of allergy by determining its siderophore-iron ligand load and thus immunomodulatory potency. Further, lipocalin proteins for use in treatment or prevention of allergies are provided, preferably complexed with siderophore-iron ligands.

Abstract: The present invention relates to a method for identifying a patient who is eligible to an intensification of heart failure therapy. Furthermore, the present invention relates to a method for optimizing BNP-type peptide guided heart failure therapy. The methods are based on the measurement of the level of at least one marker in a sample from a patient who has heart failure and who receives BNP-type peptide guided heart failure therapy. Further envisaged by the present invention are kits and devices adapted to carry out the present invention.

Abstract: Provided is a method for measuring amyloid precursor protein (APP) cleavage peptides including amyloid beta (A?) peptides.

Abstract: Methods for the diagnosis and prognosis of neurodegenerative diseases, such as Alzheimer's disease, are described. Compositions and method for the treatment of neurodegenerative diseases are also described.

Abstract: A method for diagnosing of or for determining the risk of developing Alzheimer's disease, as well as for determining the presence of severe AD in a subject involves at least four specific biomarkers being measured. A kit or an array comprising a detecting means, in particular antibodies, for at least four specific biomarkers can be used for the diagnosis. Further, a computer program product and a computer implemented method for diagnosing of or determining the risk of developing Alzheimer's disease may be employed.

Abstract: Methods for treating diseases such as cancer comprising administering a DLL4 antagonist, either alone or in combination with other anti-cancer agents, and monitoring for cardiovascular side effects and/or toxicity.

Abstract: Methods are provided for quantifying the fibroblast growth factor receptor 2 protein (FGFR2) directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry. The biological sample may be selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells. A protein sample is prepared from the biological sample and the FGFR2 protein is quantitated in the sample using the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one fragment peptide derived from FGFR2.

Abstract: Methods and kits for determining deparanase procoagulant activity in a biological sample. Diagnostic methods for the detection and/or monitoring of a coagulation-related pathological disorder in a mammalian subject. Compositions comprising heparanase and at least one tissue factor, uses and methods based thereon in the treatment, amelioration and prevention of coagulation-related pathologic conditions. Methods for screening a coagulation modulatory compound, methods and uses based thereon in the treatment, amelioration and prevention of coagulation-related pathologic conditions.

Abstract: The present invention provides a full-automatic feces analyzer, comprising an automatic controller; at least one sample box for containing a feces sample; a sample box support for placing the sample box; a dilution and stirring unit for adding diluent into the feces sample in the sample box and performing stirring to obtain feces sample liquid; a physical detection unit for performing a physical detection on the feces sample liquid; at least one chemical detection unit for performing a chemical detection on the feces sample liquid, each chemical detection unit comprising a driving roller and a driven roller, a reagent strip roll being wound on the driven roller, an end portion of the reagent strip roll being fixed on the driving roller, and the reagent strip roll comprising a plurality of reagent strips which are abreast distributed and sequentially connected; and a sample sucking and adding unit for sucking the feces sample liquid and delivering the feces sample liquid to the physical detection unit and the

Abstract: An in vitro diagnostics automation system is provided that includes a track and a plurality of sample containers configured to hold a fluid sample to be processed at one or more testing stations. The system also includes one or more priority indicators each configured to be coupled to the plurality of sample containers. Each of the one or more priority indicators includes distinguishing imaging features that indicate information associated with a fluid sample in a corresponding sample container. The system also includes an imaging device configured to image the one or more priority indicators having the distinguishing imaging features imaged by the imaging device. The system further includes a processor configured to determine the information associated with the fluid sample in the corresponding sample container from the distinguishing imaging features and control processing of the sample container based on the distinguishing imaging features imaged by the imaging device.

Abstract: When a sample of biological origin in an aqueous solution is used as the measurement medium in analysis using an electrochemical process, and a voltage of +1.2 V or greater (with saturated silver-silver chloride electrode potential as a reference) is applied, there are instances in which bubbles are observed to be produced within the flow cell, due to an electrolysis reaction deriving from the measurement buffer. There is a possibility that bubbles produced on the electrode will cover the electrode surface, reducing the effective surface area of the electrode. Also, the distribution of magnetic particles captured on the electrode will be disturbed by the gas produced thereby, lowering the reproducibility of the results of the analysis. Deaeration of the measurement medium prior to introduction of the measurement medium into the detector minimizes the effects of bubble production in degrading the analytical capability makes it possible to carry out highly sensitive electrochemical analysis.

Abstract: A rack includes a container holder that is fixed to a stage and holds a container that contains a liquid to be dispensed and a camera that is fixed to the stage and located in a position where the camera is capable of capturing an image of the container.

Abstract: Example automated diagnostic analyzers and methods for using the same are disclosed herein. An example apparatus described herein includes a first carousel rotatably coupled to a base and having a first axis of rotation. The example apparatus includes a second carousel rotatably coupled to the base and vertically spaced over the first carousel such that at least a portion of the second carousel is disposed over the first carousel. In the example apparatus, the second carousel has a second axis of rotation and a plurality of vessels. The example apparatus also includes a pipetting mechanism offset from the second axis of rotation. The example pipetting mechanism is to access the first carousel and the second carousel.

Abstract: In the past, when centering the center of a liquid surface and fixation thereof proved difficult during mounting a container into an automatic analytical apparatus, there was sometimes the possibility that the accuracy of checking the liquid surface conditions and the pipetting precision could not be ensured. When this problem occurred, there was the possibility that the reliability of the analysis results would be diminished. Accordingly, an automatic analytical apparatus is provided with a probe for suctioning a liquid to be used in analysis, wherein the apparatus is provided with a transport apparatus for transporting a container accommodating the liquid, and a container-clamping apparatus for clamping the container transported by the transport apparatus. The container-clamping apparatus has a vibration suppression mechanism for retaining the clamped container with an elastic body, and a centering retention mechanism with which centering of the container center can be fixed.

Abstract: An automatic analyzer has a washing tank providing water for washing a reagent or sample probe is. The washing water from a washing nozzle spreads from a throttle portion and is divided into right and left flows after colliding with a vent plate provided in an overflow portion of the washing tank. The washing water flows between the vent plate and the inner wall of the overflow portion, and the flows join behind the vent plate. After joining, the washing water flows downward along the vent plate and the inner wall of the overflow portion and then is drained. Because a space is created between the washing water which has collided with the vent plate and the washing water joined behind the vent plate, the washing water is prevented from completely covering the overflow portion, and the airflow can be secured during the drainage.

Abstract: Systems, methods and non-transitory, computer-readable mediums are disclosed for pedestrian pace estimation with pace change detection. In some implementations, a method comprises: receiving, by a processor of a mobile device, raw pedometer data from a pedometer included in or coupled to the mobile device; receiving, by the processor, raw GNSS data from a GNSS receiver included in or coupled to the mobile device; estimating, using a first filter implemented on the processor, a step frequency of a user wearing or holding the mobile device, the estimating including using the raw pedometer data; determining, by the processor, a pace change, the pace change determined from the estimated step frequency; responsive to the pace change, adapting by the processor, a bandwidth of a second filter implemented by the processor; and estimating, by the second filter, an estimated pace or speed of the user.

Abstract: A speed sensor system for measuring rotational speed includes a magnetic sensor and a cylindrical drum rotatable around the magnetic sensor. The cylindrical drum includes several axially extending members disposed circumferentially around a first edge of the cylindrical drum. The magnetic sensor is configured to generate an electrical output that indicates each passage of each of the axially extending members around the magnetic sensor as the cylindrical drum rotates around the magnetic sensor. The axially extending members are adapted to deflect radially outwards away from the magnetic sensor in response to an increased rotational speed of the cylindrical drum. The deflection of the axially extending members provides voltage regulation functionality that maintains voltage outputs of the magnetic sensor within a desired operational range.

Abstract: A tachometer system for an aircraft landing gear. The tachometer system includes a tachometer arranged to generate a variable voltage signal in response to the rotation of a wheel of the aircraft landing gear, and a processing system arranged to output a speed signal indicative of the rotation speed of the wheel of the aircraft landing gear. The speed signal is determined from the variable voltage signal from the tachometer using a set of determined parameters. The determined parameters of the processing system are determined from the voltage levels of the variable voltage signal.

Abstract: A touch sensor is associated with a housing that is adapted for attachment to the outer perimeter of a fluid conduit. The housing can be attached to the fluid conduit such that the touch sensor can detect the presence of fluid within the fluid conduit. The output of the touch sensor can be used by an indicator or processor.

Abstract: A mobile terminal measures sensor values in a predetermined period. And the mobile terminal detects whether a missing sensor value exists in the predetermined period. And when the mobile terminal is detected the missing sensor value, the mobile terminal interpolates the missing sensor value with a Gaussian process.

Abstract: A system and method for reducing offset in a MEMS sensor are disclosed. In a first aspect, the system is a MEMS sensor that comprises a sensing reference plane, at least one anchor coupled to the sensing reference plane, at least one proof mass coupled to the at least one anchor, wherein one of the at least one proof mass moves under an external excitation, a pattern of sensing elements coupled between the sensing reference plane and the at least one proof mass to detect motion normal to the sensing reference plane, wherein the pattern of sensing elements shares at least three axes of polarity anti-symmetry, and a signal processing circuit to combine the pattern of sensing elements thereby providing an output proportional to the external excitation. In a second aspect, the sensing reference plane is divided by two axes forming four quadrants on the sensing reference plane.

Abstract: A capacitive accelerometer including: at least one additional fixed capacitor electrode with a plurality of additional fixed capacitive electrode fingers extending along the sensing direction. The proof mass comprises a plurality of moveable capacitive electrode fingers extending from the proof mass along the sensing direction and arranged to interdigitate with the plurality of additional fixed capacitive electrode fingers of the at least one additional fixed capacitor electrode. A means is provided for applying a voltage to the at least one additional fixed capacitor electrode to apply an electrostatic force to the plurality of moveable capacitive electrode fingers that acts to pull the proof mass towards the at least one further fixed capacitor electrode and thereby reduces the lateral spacings between the movable capacitive electrode fingers of the proof mass and the first and second sets of fixed capacitive electrode fingers that provide electrostatic forces for sensing purposes.

Abstract: An accelerometer device configured for in-situ calibration applies a laser-induced pushing force at a first magnitude to a proof mass of an accelerometer, and while applying the laser-induced pushing force at the first magnitude to the proof mass, the device obtains a first output from the accelerometer. The device is further configured to apply a laser-induced pushing force at a second magnitude to the proof mass, and while applying the laser-induced pushing force at the second magnitude to the proof mass, the device obtains a second output from the accelerometer. Based on the first output and the second output, the device determines a scale factor for the accelerometer. The device is configured to determine a third output for the accelerometer, and based on the scale factor and the third output, determine an acceleration value.

Abstract: In some examples, an accelerometer may self-calibrate while in use by setting a scale factor of the accelerometer to a first value; while the scale factor of the accelerometer is set to the first value, obtaining a first acceleration value; setting the scale factor of the accelerometer to a second value; while the scale factor of the accelerometer is set to the second value, obtaining a second acceleration value; based on the first acceleration value and the second acceleration value, determining a bias correction value; obtaining a third acceleration value; and correcting the third acceleration value based on the bias correction value.

Abstract: A measuring system for performing measurements on a device under test is provided. The measuring system comprises a measuring device, a probe connected to the measuring device, a power sensor and a positioning unit. The positioning unit is adapted to connect the probe to the power sensor in a first configuration and to the device under test in a second configuration. The measuring device is adapted to generate a first signal. The probe is adapted to supply the first signal to the power sensor and the first configuration and to supply the first signal to the device under test and the second configuration. The power sensor is adapted to determine the power of the first signal and the first configuration. The measuring device is adapted to regulate the power of the first signal in the second configuration based upon the power determined by the power sensor.

Abstract: Accurate measurements of electrical power at various points of a power grid is becoming more important and, at the same time, is getting more difficult as the old power distribution model of a few, large power generating stations and a multitude of relatively linear loads is replaced by a newer model containing a multitude of smaller, and to some degree unpredictable power sources, as well as a multitude of not always linear and often smart (essentially also unpredictable) loads. Embodiments of the invention provide for management of AC current measurements in the presence of a DC current. Such current measurement management including at least alarms, feedback, and forward correction techniques exploiting magnetic field measurements from within the magnetic core or upon the surface of magnetic elements and/or shields within the current transducer.

Abstract: Some embodiments relate to power detector including a voltage sensor configured to detect a voltage of a load and a current sensor configured to detect a current of the load. The power detector also includes circuitry configured to introduce a phase delay between the detected voltage of the load and the detected current of the load, thereby producing a voltage measurement and a current measurement. The circuitry is also configured to multiply the voltage measurement and the current measurement.

Abstract: Disclosed herein are systems, methods, and devices for power and energy metering. Devices may include first processing logic coupled to an isolator and configured to receive a first bit stream from a first modulator via the isolator. The first bit stream may be generated by the first modulator based on a first analog signal. The first processing logic may be further configured to receive a second bit stream from a second modulator via the isolator. The second bit stream may be generated by the second modulator based on a second analog signal. The processing logic may be further configured to generate a third bit stream based on a combination of the first bit stream with the second bit stream. The devices may also include second processing logic configured to generate an output signal based on one or more values of the third bit stream. The output signal may characterize a measurement of electrical power associated with an electrical circuit.

Abstract: A method and an apparatus for detecting a vector shift in an AC power system, the apparatus comprising frequency measuring means (10) adapted to determine a frequency of the AC power system, derivation means (20) adapted to determine a time derivative of the frequency, filtering means (30) adapted to filter the time derivative of the frequency, and detection means (40, 50, 60, 70) adapted to calculate time derivatives for the filtered time derivatives of the frequency over a time period having the length of the fundamental period of the AC power system, to determine a number of positive and negative time derivatives among the calculated time derivatives, and to detect a vector shift in the AC power system in response to an absolute value of a difference between the determined number of positive time derivatives and negative time derivatives exceeding a predetermined threshold value.

Abstract: Energy flow in a power grid system (e.g., an electrical energy distribution system) is managed. In an example embodiment, a system provides for generating monitoring data for an electrical energy distribution system based on power-flow data for the electrical energy distribution system and topology data for the electrical energy distribution system. The power-flow data is indicative of voltage values that are repeatedly obtained from the electrical energy distribution system. The topology data is indicative of a topology for the electrical energy distribution system. The system also provides for identifying, based on the monitoring data, a change in a voltage angle associated with a transmission line in the electrical energy distribution system. Furthermore, the system provides for generating a notification for a graphical user interface in response to a determination that the change in the voltage angle for the transmission line satisfies a defined criterion.

Abstract: An approach for maximizing the resolution of a resistance sensor with a variable current source.