Abstract: Derivatives of 2-ethylidene norbornene are presented. The derivatives can be incorporated into fragrance or flavor compounds and consumer products. A method to impart, enhance or modify the odor properties of a perfuming composition or perfumed consumer product is also provided.

Abstract: A liquid laundry detergent composition that includes: a non-amine neutralized linear alkylbenzene sulphonate, a non-amine neutralized alkyl sulphate or a mixture thereof; a non-ionic surfactant; an alcohol; a hydroxyl-containing amine compound; an adjunct ingredient; water.

Abstract: A liquid laundry detergent composition that includes: a liquid phase; a solid cleaning active, where the active is selected from the group of soil-release polymer, brightener, polycarboxylate polymer or mixtures thereof, and where the solid is dispersed within the liquid phase.

Abstract: A liquid laundry detergent composition that includes a liquid phase, a lamellar liquid crystal phase, and a particulate phase, where the lamellar liquid phase and particulate phase are dispersed within the liquid phase.

Abstract: A liquid laundry detergent composition that includes: a non-amine neutralized linear alkylbenzene sulphonate; a non-ionic surfactant; a fatty acid; an alcohol; less than 5% by weight of the composition of a hydroxyl-containing amine; water; and a siloxane-based polymer suds suppressor.

Abstract: A dishwashing detergent comprising a builder; a surfactant; a first polymer comprising monoethylenically unsaturated C3-C6 carboxylic acid units and C1-C12 alkyl (meth)acrylate units; and a dispersant comprising a second polymer comprising monoethylenically unsaturated C3-C6 carboxylic acid units.

Abstract: A hand dishwashing liquid detergent composition having a surfactant system having an anionic surfactant and an amine oxide co-surfactant, a lipase, and preferably at least 0.05% by weight of the composition of at least one monovalent, divalent or trivalent cation or a mixture thereof.

Abstract: A hand dishwashing detergent composition having a surfactant system a lipase at least 0.05% by weight of the composition of at least one monovalent, divalent or trivalent cation or a mixture thereof.

Abstract: Variants with cutinase activity of a parent cutinase, comprising an alteration at one or more (e.g. several) positions corresponding to positions: 181, 182, 115, 161, 1, 2, 43, 55, 79, or 5 of SEQ ID NO: 2, wherein the alteration is a substitution for positions 181, 115, 161, 43, 55, 79, and 5, and a deletion for positions 1, 2 and 182, and wherein the variant has at least 75% but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 2. Polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and method for obtaining and methods of producing the variants. Compositions comprising the variants and methods for using the variants.

Abstract: Stabilized hydrogen peroxide-containing compositions and methods of making same are disclosed. The compositions contain a stabilizer system made up of a disulfonate surfactant, a diester solvent, and a sulfonic acid or a salt thereof in a sufficient quantity to provide the stabilized hydrogen peroxide with an acidic pH value. The compositions are suitable for use as disinfectants, as cleaning agents, and in various personal care applications such as hair care and tooth whitening.

Abstract: [Problem to be Solved] To provide a liquid laundry detergent composition for clothing which can remove bacteria from the clothing effectively during washing and also impart an excellent antibacterial effect to the clothing after washing.

Abstract: A liquid laundry detergent composition including: an aldehyde-containing fragrance material, a ketone-containing fragrance material or a mixture thereof; an alcohol a non-amine neutralised linear alkylbenzene sulphonate; a hydroxyl-containing amine; and water.

Abstract: A liquid laundry detergent composition that includes: a liquid phase; and a solid cellulosic polymer, where the solid is dispersed within the liquid phase.

Abstract: A personal care composition comprising a cosmetically acceptable base and inorganic particulate material, for example, a shower gel or hair shampoo, and a method of making the personal care composition. A cleaning composition comprising a base and inorganic particulate material, for example, a hard surface cleansing composition, and a method of making the cleaning composition. A composition, for example, a personal care composition, use of an inorganic particulate material having a d50 of from about 0.1 ?m to about 200 ?m in such a composition, and a method for making such a composition.

Abstract: A composition for treating a hard surface. The composition has: (a) at least one adhesion promoter; (b) at least one surfactant selected from the group consisting of: anionic, non-ionic, cationic, amphoteric, zwitterionic, and combinations thereof; (c) mineral oil; (d) water; (e) optionally, at least one solvent; and wherein the composition is self-adhering upon application to a surface to be treated, and wherein the composition provides a wet film to said surface when water passes over said composition and surface.

Abstract: A liquid laundry detergent composition that includes: a liquid phase; and a water-soluble solid phase, where the solid phase includes a cleaning active.

Abstract: A liquid laundry detergent composition that includes: a liquid phase; and a solid enzyme particle, where the solid particle is dispersed within the liquid phase.

Abstract: Disclosed are products incorporating fragrance-enhancing compositions, methods for increasing the fragrance intensity of fragrance-containing products, and methods for the manufacture of the fragrance-containing products. A fragrance containing gel is exposed to a space outside of the container upon opening of the container for dispensing a material from the container.

Abstract: The present invention relates to a laundry article, a water-based composition for use in the preparation of the laundry article, and methods of preparing the water-based composition and laundry article. The laundry article comprises: (i) a water-insoluble substrate; and (ii) a dried water-soluble coating provided on the substrate, the dried water-soluble coating comprising: (a) from about 60% to about 95% by weight of at least one surfactant; (b) from about 0.5% to about 5% by weight of at least one enzyme; (c) from about 1% to about 35% by weight of at least one binder; and (d) from about 0% to about 15% by weight of residual water; the percentages by weight being percentages by weight of the total dried water-soluble coating.

Abstract: The present invention provides for passive VOC recovery in the fermentation process that does not affect or minimally affects the conditions within the fermentor vessel and does not affect or minimally affects the conditions within the headspace of the fermentor vessel itself while using the production of CO2 emitted during the fermenting process as the source of driving energy to move a portion of the gaseous/vaporous material in the headspace of the fermentor through an appropriately sized conduit to a chilled surface condensing device to condense the VOCs (principally ethanol) for recovery and to exhaust the CO2 to the atmosphere or to recover the CO2 for other uses.

Abstract: An improved system and process for rapidly producing distilled spirits having characteristics associated with a much longer maturation process is provided. The method involves (a) subjecting an unmatured spirit to ultrasonic energy at a power of at least 3 Watts/Liter for at least one hour while maintaining the temperature of the unmatured spirit between 90° F. and 150° F.; and (b) subjecting the unmatured spirit and wood to actinic light at a wavelength ranging from 400 nm to 1000 nm for a cumulative exposure of at least 2,280,000 lux hours. The disclosure also provides embodiments where a spirit processed with ultrasonic energy is mixed with a spirit that has been separately treated with light to give characteristics of a mature spirit.

Abstract: A rectangular or cylindrical container made of stainless steel or another liquid-impervious material has a plurality of openings formed in the side wall(s). Wooden boards, made of white oak or other wood for aging and flavoring beverages, are fitted over the openings and sealed so that their inside surfaces contact the liquid in the container. The inside surfaces have cross-grain cuts such as saw cuts or holes, which are used to enhance the infusion of beverage liquid into the wood to age the beverage rapidly and flavorfully. When one batch is finished, the used boards are removed and new ones are fitted into the openings and used to treat another batch. Instead of, or in addition to the boards over the side-wall openings, hollow wooden air boxes having external cross-grain cuts are positioned in the container.

Abstract: A cell culture container characterized by being equipped with a container main body and a flat plate attached onto one surface of the container main body, wherein the container main body is equipped with an inflow port through which a liquid can flow into the container main body, a passage through which the liquid flowing into the container main body from the inflow port can pass, and an outflow port through which the liquid passing through the passage can flow out from the container main body, and wherein, on the bottom surface of the passage, multiple cell-seeding areas in which cells passing through the passage can be seeded are arranged side by side along the passage.

Abstract: A cell culturing device includes a culture tank, a shaft member at least partially disposed in the culture tank, a stirring mechanism supported by the shaft member, disposed in the culture tank, and a filter disposed in contact with the shaft member so as to suck the culture solution from the culture tank and/or to supply the culture solution to the culture tank. The shaft member is at least partially hollow, has openings to introduce the culture solution to the interior or to discharge the culture solution from the interior, and is unrotatable. Suction of the culture solution from the culture tank and/or supply of the culture solution to the tank is performed through the filter and the interior of the shaft member.

Abstract: A vibration generator causes a carrier to oscillate, rather than the housing of the vibration generator. The vibration generator is placed within the housing, which itself is not induced to oscillate, thus reducing or eliminating undesired noises due to excessive oscillations of the housing. The vibration generator transfers vibrations to the carrier for Petri dishes using at least one connecting element that is arranged between the carrier and the vibration generator. The connecting element protrudes through the wall of the housing and is sealed by a sealing element with respect to the wall.

Abstract: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.

Abstract: The present disclosure relates to systems and methods for flow-through separation of paramagnetic particle-bound cells in a cell suspension containing both bound and unbound cells as well as systems and methods for removing paramagnetic particles from paramagnetic particle-bound cells or from a cell suspension with unbound cells. It further relates to a flow-through magnetic separation/debeading module and a flow-through spinning membrane debeading module.

Abstract: A polymer film for packaging at least one microorganism culture medium includes at least one layer of polystyrene and at least one heat-sealing layer. The polymer film has an average water vapour permeability of between 30.0 g/m2×24 hours and 140.0 g/m2×24 hours, preferentially between 70.0 g/m2×24 hours and 120.0 g/m2×24 hours.

Abstract: A method for growing organically derived building materials in the form of a moldable substrate that can be engineered to serve a wide range of manufacturing and construction applications is presented. In particular, the embodiments consider a plurality of fungal molded shapes preferably grown from fungal inoculum and mechanically compressed at least once during the growing process as well as integration of structure support members to the fungal structure. The present invention provides a fungal substrate which could be molded, and easily and cheaply preprocessed to precise geometric specifications. The organically derived building materials also incorporate layers of structural reinforcements to improve load bearing and other structural capacities.

Abstract: This disclosure provides a three-dimensional (3D) microenvironment presenting defined biochemical and physical cues that regulate cellular behavior and use of the microenvironment. A composition to form the 3D microenvironment is provided by combining one or more natural or synthetic polymeric materials and substrate proteins recombinantly or chemically functionalized with a variety of bioactive peptides such as extracellular matrix-derived or growth factor-derived peptides. The disclosure also provides for devices and methods for screening for optimal combinations of the bioactive motifs in order to create an extracellular microenvironment that can regulate specific cellular behavior such as cell growth, proliferation, migration or differentiation.

Abstract: A method of improving quality of a blastocyst is described. The method includes removing a segment of trophectoderm from a first blastocyst. The method also includes removing a portion of trophectoderm from a second blastocyst. In addition, the method includes introducing to the second blastocyst the segment of trophectoderm from the first blastocyst. The method will promote attachment and proliferation of the trophectoderm in the second blastocyst. The first and second blastocyst will often be obtained from a same or similar period of time after insemination. The first blastocyst will have a same quality or better quality trophectoderm than the second blastocyst. The second blastocyst will include the segment of trophectoderm from the first blastocyst.

Abstract: A method for enhancing differentiation of dopaminergic (DA) neurons is provided. The method of the present invention includes culturing precursor cells; administering urocortin to the precursor cells; and differentiating the precursor cells into the DA neurons.

Abstract: Compositions and methods are disclosed herein for producing one or more immunoglobulins in an isolated cytotoxic B lymphocyte cell line. An isolated cell line includes an isolated B lymphocyte cell line capable of expressing at least one exogenously incorporated membrane immunoglobulin reactive to a first antigen and at least one endogenous secreted immunoglobulin reactive to a second antigen, and further capable of expressing at least one exogenously incorporated recombinant B cell receptor that signals for expression of cytotoxic effector molecules.

Abstract: The present invention relates to methods and kits for expanding a stem cell population. More particularly, the invention relates, inter alia, to methods, kits, and compositions for expanding a stem cell population, particularly a hematopoietic stem cell population.

Abstract: Disclosed are methods for manipulating and expanding stem cell populations, including adult stem cells, the cells produced by such methods, and various protein constructs related thereto.

Abstract: Described herein are methods and related compositions for inducing differentiation of human pluripotent stem cells (hPSCs) into hemogenic endothelium with pan-myeloid potential or restricted potential, by forced expression in the hPSCs of a combination of transcription factors as described herein.

Abstract: Disclosed herein are cell cultures and enriched cell populations of endocrine precursor cells, immature pancreatic hormone-expressing cells and mature pancreatic hormone-expressing cells. Also disclosed herein are methods of producing such cell cultures and cell populations.

Abstract: The present invention relates to an induced pluripotent stem cell (iPS) model for cardiofaciocutaneous (CFC) syndrome, a method for producing the model, and uses of the iPS model in the analysis of neural development in CFC syndrome. Specifically, the CFC syndrome-derived iPS and generation and differentiation of an embryonic body were induced from the fibroblasts of a CFC syndrome patient, and the CFC syndrome-derived iPS and embryonic body were confirmed to exhibit broken embryonic body shapes and no differentiation into neurons. When a CFC syndrome-derived embryonic body was induced by treating with p-ERK and p-SMAD1 inhibitors, the embryonic body exhibited a normal embryonic body shape and effectively differentiated into neurons. Thus, the CFC syndrome patient-derived stem cell model of the invention can be effectively used in the research for neural development in cardiofaciocutaneous syndrome.

Abstract: Provided herein are recombinant virion arrays comprising human membrane bound proteins that retain their native conformations and/or interactions, recombinant HSV-1 virions, and methods of use including high-content, high-throughput assays for screening for ligands and/or drugs that bind human membrane bound proteins, diagnostic assays, proteomic assays, and bio-sensor assays. Also provided are recombinant HSV-1 virions comprising an envelope comprising a plurality of heterologous membrane bound proteins that retain their native conformations and/or interactions as well as recombinant HSV-1 bacterial artificial chromosome (BAC) clones encoding heterologous membrane polypeptides.

Abstract: A vector, composition and method to improve influenza virus replication by inhibiting miRNA lec-7C binding to influenza virus mRNA and/or cRNA.

Abstract: A fusion protein for protein detection in which are fused: a protein domain including at least one among a C1 domain of protein G, a C2 domain of protein G, and a C3 domain of protein G; and a double mutant D153G/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 153 amino acid residue Asp has been substituted by Gly and the 330 amino acid residue Asp has been substituted by Asn, a double mutant D153H/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 153 amino acid residue Asp has been substituted by His and the 330 amino acid residue Asp has been substituted by Asn, or a double mutant K328R/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 328 amino acid residue Lys has been substituted by Arg and the 330 amino acid residue Asp has been substituted by Asn.

Abstract: Genetically engineered organophosphorus acid anhydrolases (OPAA) with improved catalytic efficiency and relaxed stereospecificity are provided. The variants typically include a mutation at the residue corresponding to H343 of wildtype Alteromonas sp. OPAA. The mutation allows the OPAA enzyme to effectively process both VR enantiomers. The OPAA optionally include one or more mutations selected the residues corresponding to Y212, V342, and I215 of wildtype Alteromonas sp. OPAA which improve the enzyme's catalytic efficiency for VX and VR. A particularly preferred OPAA includes mutations at the residues corresponding Y212F, V342L, I215Y, and H343D relative to wildtype Alteromonas sp. OPAA. Compositions including an effective amount of OPAA to increase hydrolysis of an organophosphate, and methods of use thereof for treating subjects exposed to an organophosphate, or a surface or liquid contaminated with an organophosphate are also provided.

Abstract: A method of increasing production of fatty acids comprising introducing into a host and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid molecule comprising a nucleotide sequence encoding a mutant acyl-ACP TE or a chimeric Cuphea viscosissima acyl-ACP TE; a host comprising the nucleic acid molecule; a mutant acyl-ACP TE or chimeric Cuphea viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE for at least one of its substrates comprising introducing into the plant acyl-ACP TE a substrate specificity-altering mutation; and a method of altering the level of activity of a plant acyl-ACP TE.

Abstract: The present invention provides a novel dual-function lipase variant and its application in processing of flour products. The amino acid sequence of the lipase has one of the following amino acid substitutions: P298T,P298T/H317P, P298T/H317P/V326S, P298T/T218S/S234F, P298T/H317P/P168L/A129S, P298T/S234F/K161R/V326S or the nucleotide sequence of wherein said lipase is substituted, deleted, or added based on the sequence encoding the amino acid described in (a) and has at least 80% identity with it. The variants have good performance in processing of flour products, while they can significantly whiten the bread or other products in processing of flour products and significantly increase the specific volume in bread baking process.

Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems. In particular the present invention comprehends engineered new guide architectures to be used in optimized CRISPR-Cas enzyme systems.

Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences especially for use as to nucleotide repeat disorders. Provided are delivery systems and tissues or organ which are targeted as sites for delivery especially for use as to nucleotide repeat disorders. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex or system especially for use as to nucleotide repeat disorders, as well as methods for the design and of such. Also provided are methods of directing CRISPR complex or system formation in eukaryotic cells especially for use as to nucleotide repeat disorders including with consideration of specificity for target recognition and avoidance of toxicity and editing or modifying a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.

Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems.

Abstract: The present invention provides an endonuclease I or enzymatically active fragment thereof wherein said endonuclease I has the sequence of SEQ ID No. 4 or a sequence which is at least 70% identical thereto and wherein the amino acid residue which is immediately N-terminal of the FYCGC pentapeptide motif has been substituted with a residue which is negatively charged as well as nucleic acid molecules encoding these enzymes and methods of removing contaminating polynucleotides from a sample using these enzyme.

Abstract: The present disclosure relates to CBH II chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.

Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides in, e.g., animal feed and detergents.