Patents Issued in December 8, 2016
  • Publication number: 20160355801
    Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
    Type: Application
    Filed: March 8, 2016
    Publication date: December 8, 2016
    Inventors: KELVIN WONG, JAY M. SHORT, MARK J. BURK, GRACE DESANTIS, ROBERT FARWELL, KELLY CHATMAN
  • Publication number: 20160355802
    Abstract: Compositions, systems, and methods for preparation of polypeptides having multiple iterations of non-standard amino acids are provided. The compositions and method can be used to produce recombinant proteins at a greater yield than the same or similar polypeptides made using conventional compositions, systems, and methods. Accordingly, in some embodiments, the polypeptides are ones that could not be made using conventional methods and reagents, or could not be made a sufficient yield or purity to serve a practical purpose using conventional methods and reagents. Polypeptides made using the disclosed compositions, systems, and methods are also provided.
    Type: Application
    Filed: February 6, 2015
    Publication date: December 8, 2016
    Inventors: Farren J. Isaacs, Miriam Amiram, Adrian Haimovich, Dieter Söll
  • Publication number: 20160355803
    Abstract: The present invention relates to methods of screening libraries of chimeric molecules comprising ribotoxic polypeptides, where screening is based on the interim reduction or elimination of ribotoxicity and the methods can identify cytotoxic molecules, each comprising a binding region and a ribotoxic region which jointly possess a desired assay-selectable characteristic, such as, e.g., binding to a target biomolecule, binding to a target cell, and/or cellular internalization.
    Type: Application
    Filed: February 4, 2015
    Publication date: December 8, 2016
    Applicant: Molecular Templates, Inc.
    Inventors: Eric Poma, Erin Willert, Jason Kim, Jack Higgins
  • Publication number: 20160355804
    Abstract: There is provided nucleic acids (mir-138Antimirs) for use in treating or preventing bone loss in a patient. Also there is provided a method for reducing the levels of endogenous mir-138 in a cell.
    Type: Application
    Filed: January 7, 2016
    Publication date: December 8, 2016
    Inventors: Moustapha KASSEM, Basem ABDALLAH, Hanna TAIPALEENMAEKI, Sakari KAUPPINEN, Tilde ESKILDSEN, Jan STENVANG
  • Publication number: 20160355805
    Abstract: Disclosed herein are double stranded RNA molecules which have been modified to exhibit one of the following, increased activity, enhanced nuclease stability, reduced off target activity and or reduced immunogenicity, to pharmaceutical compositions comprising such compounds and to methods of use. Further disclosed is a method for the synthesis of threose nucleic acid phosphoramidites and methods of use thereof.
    Type: Application
    Filed: January 15, 2016
    Publication date: December 8, 2016
    Inventors: Sharon Avkin-Nachum, Elena Feinstein, Leonid Beigelman
  • Publication number: 20160355806
    Abstract: This invention relates to long non-coding RNAs (lncRNAs), libraries of those ncRNAs that bind chromatin modifiers, such as Polycomb Repressive Complex 2, inhibitory nucleic acids and methods and compositions for targeting lncRNAs.
    Type: Application
    Filed: February 22, 2016
    Publication date: December 8, 2016
    Inventors: Jeannie T. Lee, Jing Zhao, Kavitha Sarma, Mark Borowsky, Toshiro Kendrick Ohsumi
  • Publication number: 20160355807
    Abstract: The present invention relates to methods for modulating angiogenesis, comprising administering to a subject, or cells or tissue derived therefrom: (i) one or more miRNA, or precursors or variants thereof, wherein at least one of said miRNA comprises a seed region comprising the sequence UCACAGU (SEQ ID NO:37) to inhibit angiogenesis; or (ii) one or more antagonists of a miRNA, wherein said miRNA comprises a seed region comprising the sequence UCACAGU (SEQ ID NO:37) to promote or induce angiogenesis. Also provided are methods of diagnosis of conditions associated with abnormal angiogenesis, or determining predisposition thereto. Suitable pharmaceutical compositions are also provided.
    Type: Application
    Filed: March 22, 2016
    Publication date: December 8, 2016
    Inventors: Jennifer Gamble, Mathew Vadas, Gregory Goodall, Jennifer Young
  • Publication number: 20160355808
    Abstract: This disclosure relates to novel huntingtin targets. Novel oligonucleotides for the treatment of Huntington's disease are also provided.
    Type: Application
    Filed: April 1, 2016
    Publication date: December 8, 2016
    Inventors: Anastasia Khvorova, Neil Aronin, Julia Alterman
  • Publication number: 20160355809
    Abstract: Provided are methods and compositions useful in the diagnosis, treatment, and monitoring of osteosarcoma. Antisense to certain microRNA (miRNA) found to be associated with cancer stem cells (CSCs) or tumor-initiating cells (TICs) of osteosarcoma are useful to suppress tumor growth and metastasis, and prolong survival. Antisense oligonucleotides to miR-133a are synergistic in combination with standard chemotherapy such as cisplatin in the treatment of osteosarcoma.
    Type: Application
    Filed: April 12, 2016
    Publication date: December 8, 2016
    Inventors: Takahiro Ochiya, Tomohiro Fujiwara
  • Publication number: 20160355810
    Abstract: The invention relates to oligonucleotides for inducing skipping of exon 51 of the dystrophin gene. The invention also relates to methods of inducing exon 51 skipping using the oligonucleotides.
    Type: Application
    Filed: April 14, 2016
    Publication date: December 8, 2016
    Inventor: Judith Christina Theodora Van DEUTEKOM
  • Publication number: 20160355811
    Abstract: Various aspects of the present invention are directed to compounds targeted to various regions of the survival motor neuron 2 (SMN2) gene. Such compounds may be used to increase incorporation of exon 7 in processed transcripts of SMN2. Such compounds may therefore be useful in increasing the amount of full-length SMN protein produced by the SMN2 gene. As such, these compounds may provide a therapeutic approach for treatment of spinal muscular atrophy (SMA).
    Type: Application
    Filed: February 10, 2015
    Publication date: December 8, 2016
    Inventors: Arthur Burghes, Vicki McGovern, Thomas Prior
  • Publication number: 20160355812
    Abstract: Compositions and methods for detecting, inhibiting, treating, and/or preventing a liver disease or disorder such as cholestasis or fibrotic liver disease are provided.
    Type: Application
    Filed: February 17, 2015
    Publication date: December 8, 2016
    Inventors: Joshua R. Friedman, Nicholas J. Hand
  • Publication number: 20160355813
    Abstract: This invention relates to long non-coding RNAs (lncRNAs), libraries of those ncRNAs that bind chromatin modifiers, such as Polycomb Repressive Complex 2, inhibitory nucleic acids and methods and compositions for targeting lncRNAs.
    Type: Application
    Filed: June 2, 2016
    Publication date: December 8, 2016
    Inventors: Jeannie T. Lee, Jing Zhao, Kavitha Sarma, Mark Borowsky, Toshiro Kendrick Ohsumi
  • Publication number: 20160355814
    Abstract: The present invention provides oligonucleotide inhibitors of miR-155 and compositions thereof. The invention further provides methods for treating cancer such as a T cell lymphoma in a subject by administering to the subject an oligonucleotide inhibitor of miR-155. The invention also provides methods for reducing or inhibiting the proliferation of malignant T cells by administering an oligonucleotide inhibitor of miR-155.
    Type: Application
    Filed: June 3, 2016
    Publication date: December 8, 2016
    Inventors: David RODMAN, Anita SETO, Christina DALBY, Aimee JACKSON, Xuan BEATTY
  • Publication number: 20160355815
    Abstract: The present invention relates to synthetic oligonucleotide mimetics of miRNAs. In particular, the present invention provides double-stranded, chemically-modified oligonucleotide mimetics of miR-29. Pharmaceutical compositions comprising the mimetics and their use in treating or preventing conditions associated with dysregulation of extracellular matrix genes, such as tissue fibrotic conditions, are also described.
    Type: Application
    Filed: June 7, 2016
    Publication date: December 8, 2016
    Inventors: Rusty L. MONTGOMERY, Christina M. DALBY, Eva VAN ROOIJ, Corrie GALLANT-BEHM
  • Publication number: 20160355816
    Abstract: Provided herein are methods for inactivating a target polynucleotide. The methods use a psiRNA having a 5? region and a 3? region. The 5? region includes, but is not limited to, 5 to 10 nucleotides chosen from a repeat from a CRISPR locus immediately upstream of a spacer. The 3? region is substantially complementary to a portion of the target polynucleotide. The methods may be practiced in a prokaryotic microbe or in vitro. Also provided are polypeptides that have endonuclease activity in the presence of a psiRNA and a target polynucleotide, and methods for using the polypeptides.
    Type: Application
    Filed: June 14, 2016
    Publication date: December 8, 2016
    Inventors: Rebecca M. Terns, Michael P. Terns, Caryn R. Hale
  • Publication number: 20160355817
    Abstract: The present invention provides RNAi agents, e.g., double stranded RNAi agents, that target the transthyretin (TTR) gene and methods of using such RNAi agents for treating or preventing TTR-associated diseases.
    Type: Application
    Filed: June 21, 2016
    Publication date: December 8, 2016
    Inventors: Kallanthottathil G. Rajeev, Tracy Zimmermann, Muthiah Manoharan, Martin Maier, Satyanarayana Kuchimanchi, Klaus Charisse
  • Publication number: 20160355818
    Abstract: The present invention provides methods for retaining and expressing physiologically active substances in a target tissue-specific-manner, by administering the physiologically active substances to target submucous tissue. Specifically, the present inventors demonstrated that, when physiologically active substances were directly administered into submucous tissues without using a carrier, the physiologically active substances were effectively and safely retained at the administration sites over long periods without loss and diffusion, and produced the effect acting in a reservoir-like fashion. The physiologically active substances administered as described above were demonstrated to produce the therapeutic effect without having an influence on organs other than the administered organ.
    Type: Application
    Filed: August 19, 2016
    Publication date: December 8, 2016
    Applicants: Stelic Institute of Regenerative Medicine, Stelic Institute & Co., Niigata University
    Inventors: HIROYUKI YONEYAMA, Kenji SUZUKI
  • Publication number: 20160355819
    Abstract: The present disclosure relates to an LNA oligonucleotide consisting of a sequence selected from the group consisting of 5?-(Tx)GxGxcsasasgscsastscscsTxGxT-3? and 5?-(Gx)TxTxascstsgscscststscsTxTxA-3?, wherein capital letters designate a beta-D-oxy-LNA nucleotide analogue, small letters designate a 2-deoxynucleotide, underline designates either a beta-D-oxy-LNA nucleotide analogue or a 2-deoxynucleotide, subscript “s” designates a phosphorothioate link between neighbouring nucleotides/LNA nucleotide analogues, and subscript “x” designates either a phosphorothioate link or a phosphorodiester link between neighbouring nucleotides/LNA nucleotide analogues, and wherein the sequence is optionally extended by up to five 2-deoxynucleotide units. The LNA oligonucleotides are useful for modulating the expression of hypoxia-inducible factor-1a (HIF-1a), e.g. in the treatment of cancer diseases, inhibiting angiogenesis, inducing apoptosis, preventing cellular proliferation, or treating an angiogenic disease, e.g.
    Type: Application
    Filed: August 22, 2016
    Publication date: December 8, 2016
    Applicant: ROCHE INNOVATION CENTER COPENHAGEN A/S
    Inventors: Majken Westergaard, Charlotte Albaek Thrue, Frank Rasmussen, Henrik Frydenlund Hansen
  • Publication number: 20160355820
    Abstract: The present disclosure relates to aptamers and uses thereof, in particular, aptamers which specifically bind to CD133 and which are particularly useful in the diagnosis and/or treatment of cancer.
    Type: Application
    Filed: February 5, 2015
    Publication date: December 8, 2016
    Inventor: Wei Duan
  • Publication number: 20160355821
    Abstract: The present invention is related to a nucleic acid molecule capable of binding to SDF-1, preferably capable of inhibiting SDF-1, whereby the nucleic acid molecule is for use in a method for the treatment and/or prevention of a disease or disorder, for use in a method for the treatment of a subject suffering from a disease or disorder or being at risk of developing a disease or disorder as an adjunct therapy, or for use as a medicament for the treatment and/or prevention of a disease or disorder, whereby the disease or disorder is cancer.
    Type: Application
    Filed: July 3, 2016
    Publication date: December 8, 2016
    Applicant: NOXXON Pharma AG
    Inventors: Werner Purschke, Florian Jarosch, Dirk Eulberg, Sven Klussmann, Klaus Buchner, Christian Maasch, Nicole Dinse, Dirk Zboralski
  • Publication number: 20160355822
    Abstract: Inhibitory oligonucleotide having the general formula: X1CCN1N2N3X2N4N5GGGN6X3N7??(I) are disclosed which can be used in pharmaceutical compositions, whereby in formula (I) C is cytidine or a derivative thereof, whereby the cytidine derivative is selected from the group consisting of 5-methylcytidine, a cytidine-like nucleotide having a chemical modification involving the cytosine base, cytidine nucleoside sugar, or both the cytosine base and the cytidine nucleoside sugar, 2?-O-methylcytidine, 5-bromocytidine, 5-hydroxycytidine, ribocytidine and cytosine-?-D-arabinofuranoside, G is guanosine or a derivative thereof, whereby the guanosine derivative is selected from the group consisting of 7-deazaguanosine, a guanosine-like nucleotide having a chemical modification involving the guanine base, the guanosine nucleoside sugar or both the guanine base and the guanosine nucleoside sugar, X1 and X3 is any nucleotide sequence with 0 to 12 bases and each nucleotide is independent of any other, X2 is any nucleoti
    Type: Application
    Filed: August 23, 2016
    Publication date: December 8, 2016
    Inventors: Eugen Uhlmann, Marion Jurk, Thomas Lehmann
  • Publication number: 20160355823
    Abstract: Nucleic acid agents for reducing or removing infestations of the Varroa destructor mite are described. Compositions comprising the nucleic acid agents and methods for controlling mite infestations using the nucleic acid agents and compositions are also disclosed.
    Type: Application
    Filed: July 2, 2014
    Publication date: December 8, 2016
    Inventors: Alan Stuart Bowman, Ewan Mclnnes Campbell, Giles Elliott Budge
  • Publication number: 20160355824
    Abstract: The present invention features methods for preventing and treating three related diseases, diet-induced obesity, metabolic syndrome, and atherosclerosis, alone or in combination by inhibiting Acyl-CoA: Cholesterol Acyltransferase 1 (ACATI) activity or expression in myeloid cells.
    Type: Application
    Filed: September 10, 2014
    Publication date: December 8, 2016
    Inventors: Ta-Yuan Chang, Catherine C.Y. Chang, Li-Hao Huang, Elaina Melton, Paul Sohn
  • Publication number: 20160355825
    Abstract: An aspect of an embodiment of the invention relates to providing treatment of disease, in particular age-related disease, through increasing or decreasing the activity of SIRT6 protein. This may be accomplished through upregulation and downregulation of expression of SIRT6 in mammals. It has been found by the inventors that mice over-expressing SIRT6 have a longer lifespan in comparison to control mice, indicating that increasing SIRT6 expression can lengthen lifespan of mammals. Agents which modulate SIRT6 expression through, for example binding to 3?UTR region of human mRNA encoding SIRT6 or by blocking binding of agents to 3?UTR region of human mRNA encoding SIRT6, have been identified.
    Type: Application
    Filed: August 22, 2016
    Publication date: December 8, 2016
    Inventors: Haim COHEN, Yariv KANFI, Sivan ELHANATI
  • Publication number: 20160355826
    Abstract: This disclosure relates to novel targets for angiogenic disorders. Novel oligonucleotides are also provided. Methods of using the novel oligonucleotides for the treatment of angiogenic disorders (e.g., preeclampsia) are also provided.
    Type: Application
    Filed: April 1, 2016
    Publication date: December 8, 2016
    Inventors: Anastasia Khvorova, Melissa Moore, Anton A. Turanov, Ananth Karumanchi
  • Publication number: 20160355827
    Abstract: General methods and strains of bacteria are described that dramatically simplify and streamline plasmid DNA production. In one preferred embodiment, endolysin mediated plasmid extraction combined with flocculation mediated removal of cell debris and host nucleic acids achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.
    Type: Application
    Filed: February 3, 2015
    Publication date: December 8, 2016
    Inventors: JAMES A. WILLIAMS, CLAGUE P. HODGSON, AARON E. CARNES
  • Publication number: 20160355828
    Abstract: The present invention relates to a novel selection system for use in a eukaryotic cell culture process and for expression of a recombinant product of interest. The selection system is based on the introduction of an exogenous functional membrane-bound folate receptor gene together with the polynucleotide or gene encoding the product of interest into a eukaryotic cell and can be widely utilized with eukaryotic cells for which cellular viability is dependent upon folic acid uptake.
    Type: Application
    Filed: August 10, 2016
    Publication date: December 8, 2016
    Inventors: Yehuda G. Assaraf, Thomas Jostock, Hans-Peter Knopf
  • Publication number: 20160355829
    Abstract: The present invention relates to a recombinant nucleic acid molecule, a recombinant microorganism, to a method for producing alanine and to the use of the recombinant nucleic acid molecule or the recombinant microorganism for the fermentative production of alanine.
    Type: Application
    Filed: September 11, 2014
    Publication date: December 8, 2016
    Applicant: BASF SE
    Inventors: Hartwig SCHRÖDER, Holger HARTMANN, Qingzhao WANG, Shakir RATANI, Zheyuan GUO, Markus POMPEJUS
  • Publication number: 20160355830
    Abstract: A microorganism of the genus Corynebacterium with an improved ability to produce L-lysine in which a septum formation initiator protein is inactivated and a method for producing L-lysine using the microorganism.
    Type: Application
    Filed: December 8, 2014
    Publication date: December 8, 2016
    Inventors: Jun Ok MOON, Sang Hee PARK, Lan HUH, Kwang Ho LEE
  • Publication number: 20160355831
    Abstract: It relates to a Mortierella alpine ATCC32222 uracil auxotroph strain and a construction method thereof. In the present invention, Mortierella alpine ATCC32222 is used as a material and undergoes gene knockout through an Agrobacterium tumefaciens mediated genetic manipulation technology, to obtain the Mortierella alpine uracil auxotroph. The method is of great significance for the basic theoretic researches of the oil producing fungus Mortierella alpine ATCC32222 and product development.
    Type: Application
    Filed: February 21, 2014
    Publication date: December 8, 2016
    Applicant: JIANGNA UNIVERSITY
    Inventors: Wei Chen, Haiqin CHEN, Yongquan CHEN, Guangfei HAO, Zhennan GU, Jianxin ZHAO, Hao ZHANG, Xiaoyun HUANG, Kai DU, Shanshan ZHAO
  • Publication number: 20160355832
    Abstract: Mesocotyl meristem explants that contain multiple primary meristems are transformed via particle bombardment or Agrobacterium-mediated methods. Regeneration is through an organogenesis pathway that allows for secondary multiple bud formation. This method allows for the genotype independent transformation of varieties of wheat.
    Type: Application
    Filed: May 6, 2016
    Publication date: December 8, 2016
    Inventors: Yinghui Dan, Joyce E. Fry
  • Publication number: 20160355833
    Abstract: Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated, target-specific RNA is provided by transcription of a chimeric gene comprising a promoter and a DNA region encoding the target-specific RNA.
    Type: Application
    Filed: July 15, 2016
    Publication date: December 8, 2016
    Inventors: Peter Michael Waterhouse, Ming-Bo Wang
  • Publication number: 20160355834
    Abstract: This invention relates to isolated nucleic acid fragments encoding polypeptides involved in post-transcriptional gene silencing. The invention also relates to construction of a recombinant DNA construct encoding all or a portion of the polypeptide involved in post-transcriptional gene silencing, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels in a transformed host cell of the the polypeptide involved in post-transcriptional gene silencing.
    Type: Application
    Filed: August 23, 2016
    Publication date: December 8, 2016
    Inventors: Rebecca E. Cahoon, HAJIME SAKAI, J ANTONI RAFALSKI
  • Publication number: 20160355835
    Abstract: The present invention relates to methods of increasing the levels of at least one sugar in developing seeds in a plant, with the methods comprising inserting an exogenous nucleic acid, which codes for at least one sugar transporter protein (SWEET protein), into a plant cell to create a transgenic plant cell, and subjecting the transgenic plant cell to conditions that promote expression of the at least one SWEET protein during seed development. The methods results in transgenic plant seeds, and transgenic plants that produce seed, where the levels of at least one sugar are increased as compared to seeds from non-transgenic plants of the same species grown under the same conditions.
    Type: Application
    Filed: March 13, 2014
    Publication date: December 8, 2016
    Applicant: CARNEGIE INSTITUTION OF WASHINGTON
    Inventor: Wolf B. Frommer
  • Publication number: 20160355836
    Abstract: Disclosed are improved plants that have increased yield. The plants show increased yield under low phosphate conditions and therefore require less fertilizer. The plants are characterised by expression of a mutant phosphate transporter gene.
    Type: Application
    Filed: February 20, 2014
    Publication date: December 8, 2016
    Inventors: Ping Wu, Jieyu Chen
  • Publication number: 20160355837
    Abstract: The invention relates to the field of plant improvement, in particular of the improvement of yield for plants, by using a transgene containing a BETL promoter driving expression of the IncW2 protein.
    Type: Application
    Filed: February 3, 2015
    Publication date: December 8, 2016
    Applicants: Biogemma, Universidad de Alcala
    Inventors: Pascual Perez, Wyatt Paul, Gregorio Hueros Soto
  • Publication number: 20160355838
    Abstract: The present invention provides methods and materials useful for improving early vigor of plants during germination. The methods and materials described herein are useful for improving early vigor of plants grown under either aerobic or anaerobic conditions. In particular embodiments described herein, the methods and materials described herein are useful for improving anaerobic germination of plants.
    Type: Application
    Filed: December 11, 2014
    Publication date: December 8, 2016
    Inventors: Endang M. Septiningsih, Tobias Kretzschmar
  • Publication number: 20160355839
    Abstract: The present disclosure provides tomato plants exhibiting resistance to Leveillula taurica (Lt) and lacking traits associated with linkage drag such as an undesirable orange fruit exocarp color. Such plants may comprise novel introgressed genomic regions associated with disease resistance from S. chilense. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.
    Type: Application
    Filed: May 6, 2016
    Publication date: December 8, 2016
    Inventors: Derek R. Drost, Elaine Graham, Albert Grit, Jacobus Hoogstraten, Stephanie Pedroni
  • Publication number: 20160355840
    Abstract: Compositions and methods useful in identifying and/or selecting maize plants that have anthracnose stalk rot resistance are provided herein. The resistance may be newly conferred or enhanced relative to a control plant. The methods use maize markers on chromosome 10 to identify, select and/or construct resistant plants. Maize plants generated by the methods also provided.
    Type: Application
    Filed: May 20, 2016
    Publication date: December 8, 2016
    Inventors: SCOTT B DAVIS, Jacso Dellai, Mark Timothy Jung, Ana Beatriz Locatelli, Petra J Wolters
  • Publication number: 20160355841
    Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.
    Type: Application
    Filed: March 14, 2016
    Publication date: December 8, 2016
    Inventors: Kenneth E. NARVA, Sarah WORDEN, Meghan FREY, Murugesan RANGASAMY, Premchand GANDRA, Balaji VEERAMANI, Wendy LO, Elane FISHILEVICH, Andreas VILCINSKAS, Eileen KNORR
  • Publication number: 20160355842
    Abstract: Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Nucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest.
    Type: Application
    Filed: May 18, 2016
    Publication date: December 8, 2016
    Applicant: AgBiome, Inc.
    Inventors: Jessica Parks, Kira Bulazel Roberts, Rebecca E. Thayer
  • Publication number: 20160355843
    Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.
    Type: Application
    Filed: July 15, 2016
    Publication date: December 8, 2016
    Applicant: INVISTA NORTH AMERICA S.A.R.L.
    Inventors: Paul S. Pearlman, Changlin Chen, Adriana Leonora Botes, Alex Van Eck Conradie
  • Publication number: 20160355844
    Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.
    Type: Application
    Filed: July 19, 2016
    Publication date: December 8, 2016
    Applicant: INVISTA NORTH AMERICA S.A.R.L.
    Inventors: Paul S. Pearlman, Changlin Chen, Adriana Leonora Botes, Alex Van Eck Conradie
  • Publication number: 20160355845
    Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.
    Type: Application
    Filed: January 4, 2016
    Publication date: December 8, 2016
    Inventors: Jun SUN, Anthony P. BURGARD, Priti PHARKYA
  • Publication number: 20160355846
    Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.
    Type: Application
    Filed: May 6, 2016
    Publication date: December 8, 2016
    Inventors: Stephen J. VAN DIEN, Anthony P. BURGARD, Robert HASELBECK, Catherine J. PUJOL-BAXLEY, Wei NIU, John D. TRAWICK, Harry YIM, Mark J. BURK, Robin E. OSTERHOUT, Jun SUN
  • Publication number: 20160355847
    Abstract: The present disclosure is directed to methods for producing butyric acid comprising fermenting a lignocellulosic biomass hydrolysate using C. tyrobutyricum strain RPT-4213 under anaerobic conditions using dilute acid-pretreated hydrolysates of wheat straw, corn fiber, corn stover, rice hull, and switchgrass, for example.
    Type: Application
    Filed: June 3, 2016
    Publication date: December 8, 2016
    Inventors: Siqing Liu, Kenneth M. Bischoff, Timothy D. Leathers, Joseph O. Rich
  • Publication number: 20160355848
    Abstract: The present technology relates to novel methods for converting algal biomass material for the production of carbon-based chemicals, in particular carboxylic acids like lactic acid using extremely thermophilic microorganisms.
    Type: Application
    Filed: May 20, 2014
    Publication date: December 8, 2016
    Applicant: DIREVO Industrial Biotechnology GmbH
    Inventors: Marco Kramer, Klaudija Milos
  • Publication number: 20160355849
    Abstract: Aspects of the invention relate to methods for producing lactic acid from organic waste, comprising contacting organic waste with a microbial community to form a fermentation mixture, and fermenting the fermentation mixture under controlled pH and temperature conditions for a time sufficient to produce lactic acid.
    Type: Application
    Filed: April 29, 2016
    Publication date: December 8, 2016
    Applicant: Massachusetts Institute of Technology
    Inventors: Gregory Stephanopoulos, Devin Hedley Currie, Kristen Jean Fortnam
  • Publication number: 20160355850
    Abstract: Modification of metabolic pathways includes genetically engineering at least one enzyme involved in elongating 2-ketoacids during leucine biosynthesis, and preferably at least isopropylmalate dehydrogenase or synthase (LeuB or LeuA in E. coli), to include at least such non-native enzyme, enzyme complex, or combination thereof to convert 2-ketobutyrate or 2-ketoisovalerate to a C7-C11 2-ketoacid, wherein the production of such is at a higher efficiency than if a purely native pathway is followed. The C7-C11 2-ketoacid may then be converted, via a native or genetically engineered thiamin dependent decarboxylase, to form a C6-C10 aldehyde having one less carbon than the C7-C11 2-ketoacid being converted. In some embodiments the C6-C10 aldehyde may then be converted via additional native or genetically engineered enzymes to form other C6-C10 products, including alcohols, carboxylic acids, and alkanes.
    Type: Application
    Filed: December 10, 2014
    Publication date: December 8, 2016
    Inventors: Paresh C. Sanghani, Brandon A. Rodriguez, Christopher C. Stowers, Amudhan Venkateswaran