Patents Issued in December 8, 2016
  • BIOLOGICAL OILS AND PRODUCTION AND USES THEREOF
    Publication number: 20160355851
    Abstract: The present invention provides biological oils and methods and uses thereof. The biological oils are preferably produced by heterotrophic fermentation of one or more microorganisms using cellulose-containing feedstock as a main source of carbon. The present invention also provides methods of producing lipid-based biofuels and food, nutritional, and pharmaceutical products using the biological oils.
    Type: Application
    Filed: August 16, 2016
    Publication date: December 8, 2016
    Inventors: James Casey LIPPMEIER, Joseph W. PFEIFER, III, Jon Milton HANSEN, III, Kirk E. APT, William Robert BARCLAY, Paul Warren BEHRENS, David Christian MARTIN
  • METHODS AND SYSTEMS FOR SULFIMIDATION OR SULFOXIMIDATION OF ORGANIC MOLECULES
    Publication number: 20160355852
    Abstract: The disclosure generally relates to the fields of synthetic organic chemistry. In particular, the present disclosure relates to methods and systems for the imidation of sulfides.
    Type: Application
    Filed: July 12, 2016
    Publication date: December 8, 2016
    Inventors: Christopher C. Farwell, John A. McIntosh, Frances H. Arnold
  • CHEMICAL ENGINEERING PROCESSES AND APPARATUS FOR THE SYNTHESIS OF COMPOUNDS
    Publication number: 20160355853
    Abstract: The present invention provides methods for producing cannabinoids and cannabinoid analogs as well as a system for producing these compounds. The inventive method is directed to contacting a compound according to Formula I or Formula II with a cannabinoid synthase. Also described is a system for producing cannabinoids and cannabinoid analogs by contacting a THCA synthase with a cannabinoid precursor and modifying at least one property of the reaction mixture to influence the quantity formed of a first cannabinoid relative to the quantity formed of a second cannabinoid.
    Type: Application
    Filed: June 2, 2016
    Publication date: December 8, 2016
    Applicant: FULL SPECTRUM LABORATORIES LIMITED
    Inventors: ROBERT WINNICKI, Marc DONSKY
  • CHEMICAL ENGINEERING PROCESSES AND APPARATUS FOR THE SYNTHESIS OF COMPOUNDS
    Publication number: 20160355854
    Abstract: The present invention provides methods for producing cannabinoids and cannabinoid analogs as well as a system for producing these compounds. The inventive method is directed to contacting a compound according to Formula I or Formula II with a cannabinoid synthase. Also described is a system for producing cannabinoids and cannabinoid analogs by contacting a THCA synthase with a cannabinoid precursor and modifying at least one property of the reaction mixture to influence the quantity formed of a first cannabinoid relative to the quantity formed of a second cannabinoid.
    Type: Application
    Filed: August 19, 2016
    Publication date: December 8, 2016
    Applicant: FULL SPECTRUM LABORATORIES LIMITED
    Inventors: Robert WINNICKI, Marc DONSKY, Mingyang SUN, Richard PEET
  • MICROORGANISMS AND METHODS FOR THE PRODUCTION OF CAPROLACTONE
    Publication number: 20160355855
    Abstract: The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.
    Type: Application
    Filed: January 13, 2016
    Publication date: December 8, 2016
    Inventors: Anthony P. BURGARD, Robin E. OSTERHOUT, Priti PHARKYA, Mark J. BURK
  • MICROBIOLOGICAL PROCESS
    Publication number: 20160355856
    Abstract: A process for the microbial synthesis of migalastat, specifically a process for the production of migalastat comprising culturing a microorganism under conditions such that at least one imino sugar is produced and detecting and/or isolating an imino sugar produced by said microorganism, and the microorganisms used in this process. The invention also comprises migalastat produced according to the above method and pharmaceutical compositions and uses thereof.
    Type: Application
    Filed: February 17, 2015
    Publication date: December 8, 2016
    Inventors: Emilio ALVAREZ-RUIZ, Andrew John COLLIS, Alison Sarah DANN, Andrew Peter FOSBERRY, Sarah Jane READY, Maria Jesus VAZQUEZ MUNIZ
  • Process for producing fibrillated cellulose material
    Publication number: 20160355857
    Abstract: The present invention relates to a process for producing a fibrillated cellulose material. In the invention fibrillated cellulose is produced enzymatically by using a low energy demanding mechanical mixer such as plough share mixer, to enhance the fibrillation. Enzymes and process conditions are chosen so that the cellulose degradation is as low as possible, while obtaining a high yield of nanofibrils. Sugars that are produced into the end-product may also be furthermore exploited.
    Type: Application
    Filed: December 18, 2014
    Publication date: December 8, 2016
    Inventors: Jaakko Hiltunen, Katariina Kemppainen, Jaakko Pere
  • METHODS OF PROCESSING SUGAR CANE AND SWEET SORGHUM WITH INTEGRATED CONVERSION OF PRIMARY AND LIGNOCELLULOSIC SUGARS
    Publication number: 20160355858
    Abstract: The current invention concerns methods and products related to the production of alcohol from sugar cane and/or sweet sorghum with integration of 1st and 2nd generation (1G/2G) biorefining, thus comprising the integrated conversion of primary and secondary soft lignocellulosic biomass. In particular, methods of processing sugar cane and/or sweet sorghum feedstock are disclosed, said methods comprising the steps of providing raw juice from the feedstock, recovering a residual bagasse, pretreating the bagasse and mixing it with some quantity of raw juice, and hydrolyzing the pretreated bagasse enzymatically.
    Type: Application
    Filed: February 11, 2015
    Publication date: December 8, 2016
    Applicants: Inbicon A/S, Odebrecht Agroindustrial SA
    Inventors: Martin Dan Jeppesen, Carlos Eduardo Calmonovici, Edmar Lopes Faleiros
  • TRANSGLUTAMINASE MEDIATED CONJUGATION OF PEPTIDES
    Publication number: 20160355859
    Abstract: Methods for conjugating peptides are provided comprising i) reacting a peptide with a first compound comprising a functional group in the presence of a transglutaminase capable of incorporating said compound into the peptide to form a transaminated peptide, and ii) reacting said transaminated peptide with e.g. a functionalized polymer capable of reacting with the functional group incorporated in the peptide in the enzymatic reaction.
    Type: Application
    Filed: June 16, 2016
    Publication date: December 8, 2016
    Inventors: NILS LANGELAND JOHANSEN, MAGALI ZUNDEL, FLORENCIO ZARAGOZA DORWALD
  • CRZ1 MUTANT FUNGAL CELLS
    Publication number: 20160355860
    Abstract: The present invention provides CRZ1 mutant fungal host cells, such as Pichia pastoris. The mutant fungal host cells exhibit temperature-resistance, enhanced fermentation robustness and increased expression of heterologous polypeptides such as immunoglobulins. Methods for producing heterologous polypeptides, such as immunoglobulins, using such mutant fungal host cells are within the scope of the present invention.
    Type: Application
    Filed: August 22, 2016
    Publication date: December 8, 2016
    Applicant: MERCK SHARP & DOHME CORP.
    Inventors: Bo Jiang, Jun Zhuang
  • METHOD FOR PRODUCING GAMMA-GLUTAMYLVALYLGLYCINE OR A SALT THEREOF
    Publication number: 20160355861
    Abstract: A method for producing ?-Glu-Val-Gly comprising the step of reacting Val-Gly with a ?-glutamyl group donor in the presence of a ?-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate ?-Glu-Val-Gly, wherein the ?-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
    Type: Application
    Filed: August 12, 2016
    Publication date: December 8, 2016
    Applicant: Ajinomoto Co., Inc.
    Inventors: Hiroyuki NOZAKI, Isao Abe, Jun Takakura, Rie Takeshita, Hideyuki Suzuki, Shunichi Suzuki
  • MULTI-MEDIATOR REAGENT FORMULATIONS FOR USE IN ELECTROCHEMICAL DETECTION
    Publication number: 20160355862
    Abstract: The present disclosure provides, inter alia, mediator formulations for the measurement of an analyte comprising at least one ruthenium compound and at least one osmium compound. Also disclosed are reagent formulations for the measurement of an analyte comprising at least one ruthenium compound and at least one osmium compound. Also disclosed are methods and devices for the measurement of an analyte in a sample.
    Type: Application
    Filed: June 3, 2016
    Publication date: December 8, 2016
    Inventors: David Zhi Deng, Gary T. Neel
  • METHOD FOR DETERMINING WHETHER OR NOT TEST SAMPLE CONTAINS PHYTOPATHOGENIC OOMYCETE
    Publication number: 20160355863
    Abstract: Provided is a method for determining whether or not a test sample contains a phytopathogenic oomycete selectively from two kinds of oomycetes of a phytopathogenic oomycete and a non-phytopathogenic oomycete. The present method comprises: (a) putting the test sample on a front surface of a film comprising a through-hole having a cross-sectional area of more than 7.065 square micrometers and not more than 19.625 square micrometers; (b) leaving the test sample at rest after the step (a); (c) observing a back surface of the film after the step (b); and (d) determining that the test sample contains the phytopathogenic oomycete, if an oomycete is found on the back surface of the film in the step (c).
    Type: Application
    Filed: September 2, 2015
    Publication date: December 8, 2016
    Inventor: YOSHITSUGU URIU
  • METHOD FOR DETERMINING WHETHER OR NOT TEST SAMPLE CONTAINS PHYTOPATHOGENIC OOMYCETE
    Publication number: 20160355864
    Abstract: Provided is a method for determining whether or not a test sample contains a phytopathogenic oomycete selectively from two kinds of oomycetes of a phytopathogenic oomycete and a non-phytopathogenic oomycete. The present method comprises: (a) putting the test sample on a front surface of a film comprising a through-hole having a cross-sectional area of not less than 0.785 square micrometers and not more than 7.065 square micrometers; (b) leaving the test sample at rest after the step (a); (c) observing a back surface of the film after the step (b); and (d) determining that the test sample contains the phytopathogenic oomycete, if an oomycete is found on the back surface of the film in the step (c).
    Type: Application
    Filed: September 4, 2015
    Publication date: December 8, 2016
    Inventor: YOSHITSUGU URIU
  • IDENTIFICATION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)
    Publication number: 20160355865
    Abstract: The invention provides a method for identifying methicillin resistant Staphylococcus aureus (MRSA) in a bacterial sample comprising the steps: classifying bacteria in the sample as Staphylococcus aureus (SA) and determining the presence or absence of the phenol soluble modulin peptide or a variant thereof wherein the presence of the PSM-mec peptide or variant thereof indicates methicillin resistant Staphylococcus aureus. The variant is preferably the formylated version of the PSM-mec peptide having a mass to charge ratio of 2415 in a singly protonated state.
    Type: Application
    Filed: December 18, 2014
    Publication date: December 8, 2016
    Inventors: Gabriele BIERBAUM, Christiane SZEKAT, Nahed ALSABTI, Michaele JOSTEN, Marion REIF
  • LABEL FREE BIOSENSORS, GRAM-NEGATIVE BACTERIA DETECTION, AND REAL-TIME AND END POINT DETERMINATION OF ANTIBIOTIC EFFECTS
    Publication number: 20160355866
    Abstract: Real-time and end point determination of antibiotic effects are disclosed herein. In one example, a surface of a label free biosensor is exposed to a sample including a gram-negative bacteria. A frequency and/or a current of the biosensor is then allowed to reach a constant value. The surface of the biosensor is then exposed to an antibiotic. Using the biosensor, i) a frequency change versus time and a damping resistance versus time, or ii) a current versus voltage or the current versus time at a fixed potential, or iii) both i and ii are then measured. The frequency change versus time and the damping resistance versus time and/or the current versus voltage or the current versus time are correlated to determine an effect of the antibiotic on the gram-negative bacteria. Examples of the label free biosensors and methods for detecting gram-negative bacteria using the label free biosensors are also disclosed.
    Type: Application
    Filed: December 31, 2015
    Publication date: December 8, 2016
    Inventor: Xiangqun Zeng
  • ASSAY AND METHOD FOR QUANTITATING CARBONIC ANHYDRASE ACTIVITY AND ASSESSING RED BLOOD HEMOLYSIS
    Publication number: 20160355867
    Abstract: A method and assay for using carbonic anhydrase (CA), particularly CA-I or CA-II, as a biomarker of hemolysis. The method and assay detect hemolysis by determining a percentage erythrocyte hemolysis in a specimen or sample of blood based upon quantification of carbonic anhydrase present in the extracellular portion of the blood. The method and test serve to optimize therapeutic efficacy for treatments of hemolysis.
    Type: Application
    Filed: June 6, 2016
    Publication date: December 8, 2016
    Inventors: Ryan R. Geyer, Pan Zhao, Mark D. Parker, Walter F. Boron
  • MUTATED GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF
    Publication number: 20160355868
    Abstract: Secreted luciferases which are different from those known heretofore have been desired. The present invention provides a luciferase mutant comprising an amino acid sequence in which at least one amino acid selected from amino acids at the positions of 1 to 4 is deleted in the amino acid sequence of SEQ ID NO: 2.
    Type: Application
    Filed: August 22, 2016
    Publication date: December 8, 2016
    Applicant: JNC Corporation
    Inventors: Satoshi INOUYE, Junichi SATO
  • BIOSENSORS INCLUDING METALLIC NANOCAVITIES
    Publication number: 20160355869
    Abstract: Devices and methods relating to biological assays are provided. In one exemplary aspect, a detection-enhancement element for a biological assay can include a substrate, a metallic layer on at least one surface of the substrate and including at least one nanocavity, a transparent film positioned between the substrate and the metallic layer; and capture molecules within the at least one nanocavity. The nanocavities are configured to enhance signals that are representative of the presence or amount of one or more analytes in a sample or sample solution, and may be configured to enhance the signal by a factor of about two or more or by a factor of about three or more.
    Type: Application
    Filed: January 12, 2015
    Publication date: December 8, 2016
    Applicant: University of Utah Research Foundation
    Inventors: Steven M. Blair, Farhad Mahdavi, Yongdong Liu, James N. Herron
  • GENERATION OF LIGATION-READY DNA AMPLICONS
    Publication number: 20160355870
    Abstract: The invention is directed to novel methods, kits and uses to be employed for the generation of ligation-ready DNA amplicons of a target DNA by using 5?-phosphorylated primers.
    Type: Application
    Filed: October 13, 2014
    Publication date: December 8, 2016
    Applicant: QIAGEN GMBH
    Inventors: Nan Fang, Dirk Loeffert
  • METHODS AND COMPOSITIONS FOR DETECTING BACTERIAL CONTAMINATION
    Publication number: 20160355871
    Abstract: Compositions, reactions mixtures, kits, and systems for detecting bacterial contamination are provided, as well as methods of using the same.
    Type: Application
    Filed: July 23, 2014
    Publication date: December 8, 2016
    Inventors: James Jian Quan Want, Xiangmin Cui, HiuNam Chan
  • GENETIC MARKERS FOR DIAGNOSIS OF TUBERCULOSIS CAUSED BY MYCOBACTERIUM TUBERCULOSIS
    Publication number: 20160355872
    Abstract: The application is related to novel signature sequences for diagnosis of Mycobacterium tuberculosis in clinical samples with 100% specificity and a very high degree of sensitivity.
    Type: Application
    Filed: December 1, 2014
    Publication date: December 8, 2016
    Inventors: Syed Asad RAHMAN, Javeed AHMAD, Nasreen Z EHTESHAM, Seyed E HASNAIN
  • REDUCTION OF BIAS IN GENOMIC COVERAGE MEASUREMENTS
    Publication number: 20160355873
    Abstract: Methods are provided for detecting and quantitating molecules using fluidics. In some embodiments, the methods comprise minimizing or eliminating biases caused by label density, or minimizing or eliminated biases caused by factors other than label density. In some embodiments, the methods comprise automated identification of genetic structural variation. In some embodiments, the methods comprise analyzing blood to detect the presence of circulating DNA or cells from a fetus or tumor.
    Type: Application
    Filed: February 24, 2015
    Publication date: December 8, 2016
    Inventor: Zeljko Dzakula
  • MULTITAG SEQUENCING ECOGENOMICS ANALYSIS
    Publication number: 20160355874
    Abstract: Embodiments of the invention herein described relate to multiplex polynucleotide sequence analysis without the use of size separation methods or blotting. In certain particulars the invention relates to multiplex sequencing using massively parallel sequencing methods, such as pyrosequencing methods and sequencing by synthesis. The invention provides increased throughput, increased accuracy of enumerating sample components, and the ability to analyze greater numbers of samples simultaneously or serially on presently available systems, as well as others yet to be developed. In certain of its embodiments the invention relates to the analysis of complex microbial communities, particularly to in-depth analysis thereof in large numbers of samples.
    Type: Application
    Filed: August 18, 2016
    Publication date: December 8, 2016
    Inventor: Patrick M. Gillevet
  • METHODS AND KITS FOR DETECTING NUCLEIC ACID SEQUENCES OF INTEREST USING DNA-BINDING PROTEIN DOMAIN
    Publication number: 20160355875
    Abstract: The present invention relates to a method for the detection of a specific nucleic acid. Specifically, the invention provides a method and kits for detecting the presence of a specific nucleic acid using engineered DNA-binding domains such as Transcription Activator Like-Effector (TALE) domain or modular base-per-base binding domains (MBBBD). The method of the invention is particularly useful for in vitro diagnostic application.
    Type: Application
    Filed: October 10, 2014
    Publication date: December 8, 2016
    Inventors: Julien VALTON, Alexandre JUILLERAT, Fayza DABOUSSI, Marine BEURDELEY, Claudia BERTONATI, Philippe DUCHATEAU
  • ASSAY DEVICES AND ASSAY ASSEMBLIES
    Publication number: 20160355876
    Abstract: An assay assembly comprising a main housing and a capture device held inside the main housing for capturing a specific biomolecule and has a detection surface is disclosed. The main housing comprises an analyte inlet, an analyte outlet and a hybridization compartment, and the capture device is held inside the hybridization compartment with the detection surface fully extended inside the hybridization compartment. The analyte inlet is connected to an inlet end of the hybridization compartment by an analyte inlet channel and the analyte outlet is connected to an outlet end of the hybridization compartment by an analyte outlet channel.
    Type: Application
    Filed: August 17, 2016
    Publication date: December 8, 2016
    Inventor: Joseph TAM
  • Methods of Detecting Target Nucleic Acids
    Publication number: 20160355877
    Abstract: The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.
    Type: Application
    Filed: August 19, 2016
    Publication date: December 8, 2016
    Inventors: Muhammad A. SHARAF, Timothy WOUDENBERG, Khairuzzaman Bashar MULLAH
  • Method for Generating of Oligonucleotide Arrays Using In Situ Block Synthesis Approach
    Publication number: 20160355878
    Abstract: The idea of this invention is to prepare ordered oligonucleotides arrays from two or more pre-synthesized shorter parts—block-synthesis approach. The parts are linked together enzymatically to form a full-length oligonucleotide of a desired sequence. Such an approach allows splitting the oligonucleotide sequences into common and unique parts. It gives the possibility to place the functional group on a common part and to minimize the length of the unique parts. Method of the invention allows combinatorial synthesis of position-specific regions. Using combinatorial approach, position-specific regions are generated by linking two or more unique oligonucleotides, so that just few said unique oligonucleotides give rise to a large variety of codes, for example, 10 unique oligonucleotides linked pairwise can produce 100 position-specific regions.
    Type: Application
    Filed: December 8, 2014
    Publication date: December 8, 2016
    Applicants: Alacris Theranostics GMBH, Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V.
    Inventors: Hans LEHRACH, Tatjana BORODINA, Vera RYKALINA
  • AMPLIFICATION AND ANALYSIS OF WHOLE GENOME AND WHOLE TRANSCRIPTOME LIBRARIES GENERATED BY A DNA POLYMERIZATION PROCESS
    Publication number: 20160355879
    Abstract: The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.
    Type: Application
    Filed: July 22, 2016
    Publication date: December 8, 2016
    Applicant: Rubicon Genomics, Inc.
    Inventors: Emmanuel KAMBEROV, Tong SUN, Eric BRUENING, Jonathon H. PINTER, Irina SLEPTSOVA, Takao KURIHARA, Vladimir L. MAKAROV
  • Method of preparing libraries of template polynucleotides
    Publication number: 20160355880
    Abstract: The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5? ends and at their 3? ends.
    Type: Application
    Filed: June 3, 2016
    Publication date: December 8, 2016
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Niall Anthony Gormley, Geoffrey Paul Smith, David Bentley, Roberto Rigatti, Shujun Luo
  • DNA Methylation Analysis
    Publication number: 20160355881
    Abstract: Disclosed herein are methods for determining whether one or more CpG dinucleotides are methylated using Linear After the Exponential Polymerase Chain Reaction (“LATE-PCR”) or Linear-Expo-Linear Polymerase Chain Reaction (“LEL-PCR”).
    Type: Application
    Filed: July 24, 2014
    Publication date: December 8, 2016
    Inventors: Lawrence J. Wangh, Jesus A Sanchez
  • NANOCHANNEL COMPOSITIONS AND METHODS
    Publication number: 20160355882
    Abstract: Disclosed herein is a composition comprising a nanochannel and a contained substance, wherein the nanochannel comprises a single-file nanochannel and the contained substance comprises a plurality of substance particles arranged in a single-file chain within the nanochannel. Methods and systems for molecular transport of a substance through a nanochannel are also provided that rely upon the use of nanojumps, where nanojumps mediate the transport through the nanochannel.
    Type: Application
    Filed: January 16, 2015
    Publication date: December 8, 2016
    Inventors: Seth Harvey LICHTER, Thomas Brian SISAN
  • METHOD FOR DETECTING GENES SENSITIVE TO HIGH-LEVEL IONIZING RADIATION, AND GENE DETECTED BY THE METHOD
    Publication number: 20160355883
    Abstract: A method for detecting genes sensitive to high-level ionizing radiation and genes detected by the method. More specifically, genes sensitive to high-level ionizing radiation discovered in a carcinogenic entity and verified in a normal entity are detected, by subjecting a cancerous AKR/J mouse and a normal ICR mouse to high-level radiation. Thymus is collected therefrom and fatty acid metabolism-related genes are classified via microarray processing of the thymus. The genes are amplified and the levels of gene expression are measured. Thus, a gene having a specific reaction to radiation can be accurately detected by preventing the interference of confounding variables.
    Type: Application
    Filed: May 17, 2012
    Publication date: December 8, 2016
    Inventors: Hee Sun Kim, Seung Jin Choi, Moo Hyun Choi, Jin Jong Bong, Seok Cheol Shin
  • GENE SIGNATURES FOR CANCER PROGNOSIS
    Publication number: 20160355884
    Abstract: Biomarkers and methods using the biomarkers for the prediction of the recurrence risk of cancer in a patient are provided.
    Type: Application
    Filed: March 3, 2016
    Publication date: December 8, 2016
    Inventors: Steven Stone, Alexander Gutin, Susanne Wagner, Julia E. Reid
  • GENE METHYLATION BASED COLORECTAL CANCER DIAGNOSIS
    Publication number: 20160355885
    Abstract: The invention provides method of identifying a colorectal cancer in a sample comprising DNA from a subject, determining the methylation status of one or more of the genes selected from BOLL, CDX1, CLIC4, DCC, ESR1, GATA4, GDNF, HLA-G, JUB, MYOD1, NKX2-1, PENK, PITX2, PTGS2, RARB, S100A2, SALL3, SEZ6L, SFRP2, SPARC, TCEB2, TFPI2, THBD, TJP2, TMEFF2, TP53, TWIST1, WT1 or combinations thereof and comparing the methylation status with a control, thereby identifying colorectal cancer DNA in the sample and means for performing the inventive method.
    Type: Application
    Filed: December 19, 2014
    Publication date: December 8, 2016
    Inventors: Andreas WEINHÄUSEL, Walter PULVERER
  • METHODS FOR MEASURING BIOMARKERS IN GASTROINTESTINAL CANCER
    Publication number: 20160355886
    Abstract: The present invention is directed to methods for determining the activity of a promoter. The present invention further describes a method for determining the susceptibility of a subject to cancer and biomarkers for detecting cancer in a subject.
    Type: Application
    Filed: December 30, 2014
    Publication date: December 8, 2016
    Inventors: Boon Ooi Patrick TAN, Masafumi MURATANI, Aditi QAMRA
  • GENE EXPRESSION PROFILING FOR THE DIAGNOSIS OF PROSTATE CANCER
    Publication number: 20160355887
    Abstract: Methods for diagnosing the presence of a disorder, such as prostate cancer, in a subject are provided, such methods including detecting the relative frequency of expression of RNA biomarkers in a biological sample obtained from the subject, for example, using NGS technology and comparing the relative levels of expression with predetermined threshold levels. Levels of expression of at least two of the RNA biomarkers that are above or below the predetermined threshold levels are indicative of the presence of prostate cancer in the subject. Also provided is a method for preparing a reference standard for quantitating the relative frequency of expression of RNA biomarkers in a biological sample obtained from the subject with a prostate cancer lesion using NGS technology.
    Type: Application
    Filed: June 1, 2016
    Publication date: December 8, 2016
    Inventors: James Douglas Watson, Clare Elton, David Rex Musgrave, Helene Belanger, Kay Alison Appleyard, Kristen Chalmet
  • METHODS OF STRATIFYING PATIENTS FOR TREATMENT WITH RETINOIC ACID RECEPTOR-ALPHA AGONISTS
    Publication number: 20160355888
    Abstract: The invention provides methods that define cellular populations that are sensitive to RARA agonists and identify patient subgroups that will benefit from treatment with RARA agonists. The invention also provides packaged pharmaceutical compositions that comprise a RARA agonist and instructions for determining if such agonist is suitable for use in treatment.
    Type: Application
    Filed: June 2, 2016
    Publication date: December 8, 2016
    Inventors: Mei Wei Chen, Cindy Collins, Matthew Lucas Eaton, Matthew G. Guenther, Nan Ke, Jeremy Lopez, Michael R. McKeown, David A. Orlando
  • METHOD TO ASSESS PROGNOSIS AND TO PREDICT THERAPEUTIC SUCCESS IN CANCER BY DETERMINING HORMONE RECEPTOR EXPRESSION LEVELS
    Publication number: 20160355889
    Abstract: The present invention is related to a method of classifying a sample of a patient who suffers from or being at risk of developing cancer, said method comprising the steps of determining in said sample from said patient, on a non protein basis, the expression level of at least one gene encoding for a hormone receptor selected from the group comprising estrogen receptor, progesterone receptor and/or androgen receptor in said sample; comparing the one or more expression level(s) determined with one or more expression level(s) of one or more reference genes, and classifying the sample of said patient from the outcome of the comparison into one of at least two classifications.
    Type: Application
    Filed: June 10, 2016
    Publication date: December 8, 2016
    Inventor: Ralph Wirtz
  • CELL PREPARATIONS AND CELL SUPPORTS AND THEIR USE IN THERANOSIS
    Publication number: 20160355890
    Abstract: A method of preparing cells from a patient specimen comprising a tissue or a clump of cells; a method of in situ hybridization (ISH) or immunohistochemistry (IHC); a method of theranosing a patient's response to an active agent that targets the tyrosine kinase pathway; an improvement of a method of theranosis comprising the method of ISH or IHC; a cell support on the surface of which are cells, which have been contacted with detectably labeled probes with the method of ISH or IHC; and a kit comprising (a) a cell support and/or at least one probe for ISH or IHC, and (b) instructions for carrying out the method of ISH or IHC on a cell support.
    Type: Application
    Filed: August 19, 2016
    Publication date: December 8, 2016
    Inventors: Irina Sokolova, John Schulz, Olga Kornfeld, Jeffrey Panella, Minghao Song, Svetlana Sitailo, Frank Policht, Gerry Browne, Ekaterina Pestova
  • EXPRESSION OF ETS RELATED GENE (ERG) AND PHOSPHATASE AND TENSIN HOMOLOG (PTEN) CORRELATES WITH PROSTATE CANCER CAPSULAR PENETRATION
    Publication number: 20160355891
    Abstract: The disclosure provides methods for characterizing a prostate cancer sample by detecting expression of ERG, PTEN or both, changes in which relative to a normal control are shown herein to be correlated with prostate cancer capsular penetration and more aggressive forms of prostate cancer. Such methods are useful for the prognosis of prostate cancer capsular penetration and for making treatment decisions in patients with prostate cancer that has penetrated the capsule. Also provided are kits that can be used with such methods.
    Type: Application
    Filed: August 19, 2016
    Publication date: December 8, 2016
    Applicants: Ventana Medical Systems, Inc., The Arizona Board of Regents on behalf of the University of Arizona
    Inventors: Gary Pestano, Ray B. Nagle, Connie Cortez, Kristie A. Vanpatten, Amit M. Algotar
  • DETECTING NEOPLASM
    Publication number: 20160355892
    Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as pancreatic and colorectal cancer.
    Type: Application
    Filed: August 22, 2016
    Publication date: December 8, 2016
    Inventors: David A. Ahlquist, John B. Kisiel, William R. Taylor, Tracy C. Yab, Douglas W. Mahoney
  • METHOD OF SEQUENCE DETERMINATION USING SEQUENCE TAGS
    Publication number: 20160355893
    Abstract: The invention is directed to the use of sequence tags to improve sequence determination of amplicons of related sequences, particularly large and complex amplicons, such as those comprising recombined nucleic acids encoding immune receptor molecules. In one aspect, sequence reads having the same sequence tags are aligned after which final base calls are determined from a (possibly weighted) average base call from sequence read base calls at each position. Similarly, in another aspect, sequence reads comprising series of incorporation signals are aligned by common sequence tags and base calls in homopolymer regions are made as a function incorporation signal values at each “flow” position.
    Type: Application
    Filed: March 15, 2013
    Publication date: December 8, 2016
    Inventors: Malek Faham, Martin Moorhead, Thomas Willis, Jianbiao Zheng
  • Endpoint Zygosity Assay To Detect RF4 Gene In Maize
    Publication number: 20160355894
    Abstract: A method is provided for determining the zygosity of an Rf4 gene in a corn plant. A method may include performing a first PCR assay, a second PCR assay, quantifying probes used in the first and second PCR assays, and comparing the quantified probes to determine zygosity.
    Type: Application
    Filed: July 23, 2013
    Publication date: December 8, 2016
    Inventors: Ruihua REN, Peizhong ZHENG, Siva Prasad KUMPATLA
  • MICRORNA POLYMORPHISMS CONFERRING ENHANCED DROUGHT TOLERANCE IN A PLANT
    Publication number: 20160355895
    Abstract: Methods of identifying a single nucleotide polymorphism associated with a plant trait and methods of identifying a plant having an improved trait. The plant trait is correlated with at least one single nucleotide polymorphism in a microRNA region of a plant genome. Isolated nucleic acids, transgenic plants, and methods of producing the same are also disclosed.
    Type: Application
    Filed: August 22, 2016
    Publication date: December 8, 2016
    Applicant: Syngenta Participations AG
    Inventors: Dale Wayne SKALLA, Joseph Dallas CLARKE, V, Ju-Kyung YU, Daolong WANG, Jianwei LU
  • INCOATED RNA
    Publication number: 20160355896
    Abstract: The present invention provides methods for the detection of RNA in a sample, comprising (a) mixing a specific amount of a rod-shaped virus-like particle with a sample, wherein the rod-shaped virus-like particle comprises a ribonucleic acid molecule and a viral coat, wherein: (i) the ribonucleic acid molecule comprises an origin-of-assembly sequence of a rod-shaped RNA virus and a heterologous sequence; and (ii) the viral coat comprises at least one type of coat protein of the rod-shaped RNA virus; (b) isolating RNA from the sample; and (c) detecting RNA comprising the heterologous sequence.
    Type: Application
    Filed: February 10, 2015
    Publication date: December 8, 2016
    Inventors: Guido Krupp, Peter Scheinert, Holger Jeske, Christina Wege
  • COMPOSITIONS AND METHODS FOR DETECTING ENTEROVIRUS D68
    Publication number: 20160355897
    Abstract: Disclosed herein are methods and compositions for detection of enterovirus D in a sample, particularly detection of enterovirus D68. The methods include contacting a sample with at least one primer (such as a forward primer and/or a reverse primer) capable of specifically amplifying an EV-D68 viral protein 1 (VP1) nucleic acid or a portion thereof and/or a detectably labeled probe capable of specifically hybridizing to an EV-D68 VP1 nucleic acid, under conditions sufficient for specific amplification of the EV-D68 VP1 nucleic acid by the at least one primer and/or under conditions sufficient for specific hybridization of the probe to the EV-D68 nucleic acid. The amplification of the EV-D68 VP1 nucleic acid and/or the hybridization of the probe to the EV-D68 VP1 nucleic acid is detected, thereby identifying presence of EV-D68 in the sample.
    Type: Application
    Filed: June 3, 2016
    Publication date: December 8, 2016
    Inventors: William Allan Nix, M. Steven Oberste
  • METHOD OF FABRICATING AN ARTICLE FOR MAGNETIC HEAT EXCHANGER
    Publication number: 20160355898
    Abstract: In an embodiment, a method of fabricating a working component for magnetic heat exchange comprises arranging at least two articles comprising a magnetocalorically active phase and an elongated form with a long axis having a length l and a shortest axis having a length s, wherein l?1.5 s, such that the shortest axes of the at least two articles are substantially parallel to one another and securing the at least two articles in a position within the working component such that the shortest axes of the at least two articles are substantially parallel to one another within the working component.
    Type: Application
    Filed: June 2, 2016
    Publication date: December 8, 2016
    Inventors: Hugo Abdiel VIEYRA VILLEGAS, Matthias KATTER, Barcza ALEXANDER
  • HOT-ROLLED STEEL SHEET FOR GAS NITROCARBURIZING AND MANUFACTURING METHOD THEREOF
    Publication number: 20160355899
    Abstract: In a hot-rolled steel sheet, an average pole density of an orientation group of {100}<011> to {223}<110>, which is represented by an arithmetic average of pole density of each orientation of {100}<011>, {116}<110>, {114}<110>, {112}<110>, and {223}<110> in a center portion of a sheet thickness which is a range of the sheet thickness of ? to ? from a surface of the steel sheet, is 1.0 or more and 4.0 or less, the pole density of a crystal orientation of {332}<113> is 1.0 or more and 4.8 or less, an average grain size in a center in the sheet thickness is 10 ?m or less, and a microstructure includes, by a structural fraction, pearlite more than 6% and ferrite in the balance.
    Type: Application
    Filed: August 17, 2016
    Publication date: December 8, 2016
    Applicant: Nippon Steel & Sumitomo Metal Corporation
    Inventors: Tatsuo YOKOI, Hiroshi SHUTO, Riki OKAMOTO, Nobuhiro FUJITA, Kazuaki NAKANO, Takeshi Yamamoto
  • PROCESS FOR MANUFACTURING COLD-ROLLED AND ANNEALED STEEL SHEET WITH A VERY HIGH STRENGTH, AND SHEET THUS PRODUCED
    Publication number: 20160355900
    Abstract: The present invention provides a cold-rolled and annealed steel sheet with a strength greater than 1200 MPa, the composition of which includes, the contents being expressed by weight: 0.10%?C?0.25%, 1%?Mn?3%, A?0.010%, Si?2.990%, S?0.015%, P?0.1%, N?0.008%, it being understood that 1%?Si+Al?3%, it being understood that Cr+3Mo?0.3%, Ti in an amount such that Ti/N?4 and Ti?0.040%. A balance of the composition includes iron and inevitable impurities resulting from the smelting. The microstructure of the steel includes 15 to 90% bainite, the remainder includes martensite and residual austenite.
    Type: Application
    Filed: August 22, 2016
    Publication date: December 8, 2016
    Inventors: Javier Gil Otin, Antoine Moulin