Abstract: The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AA V) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.

Abstract: This application describes methods, including non-naturally occurring methods, for biosynthesizing unsaturated pentahydrocarbons, such as isoprene and intermediates thereof, via the mevalonate pathway, as well as non-naturally occurring hosts for producing isoprene.

Abstract: The use of a product for internal dehydration of hydrogenated sugar as a methanogen substrate in a method for biogas production, a composition including a monoanhydrohexitol (M), a dianhydrohexitol (D), and anhydrohexitol polymers (P), and a methanisation method.

Abstract: The invention relates to improved processes of producing ethanol from starch-containing material wherein saccharification and/or fermentation is done at a temperature below the initial gelatinization temperature in the presence of glucoamylase and alpha-amylase, and optionally a protease and/or a cellulolytic enzyme composition; wherein the fermenting organism is Saccharomyces cerevisiae MBG4851 (deposited under Accession No. V14/004037 at National Measurement Institute, Victoria, Australia) or a fermenting organism strain having properties that are about the same as that of Saccharomyces cerevisiae MBG4851, or a derivative of Saccharomyces strain V14/004037 having defining characteristics of strain V14/004037, and compositions comprising a Saccharomyces yeast strain of the invention and naturally occurring and/or non-naturally occurring components.

Abstract: A solid lignocellulosic fuel composition is produced from combining the syrup co-product of a lignocellulosic biomass fermentation process and an additional fuel component. The syrup is an excellent binder for a powdery fuel material that is not readily handled. The fuel composition is further processed to form briquettes, pellets and the like.

Abstract: The present invention relates to the fermentative production of alcohols including ethanol and butanol, and processes for improving alcohol fermentation employing in situ product removal methods.

Abstract: The present invention relates to a recombinant microorganism useful for the production of 1,2-propanediol and process for the preparation of 1,2-propanediol. The microorganism of the invention is modified in a way that the 1,2-propanediol production is improved by enhancing NADPH dependent HAR activity.

Abstract: The invention provides a recombinant microorganism that has been genetically engineered to contain metabolic pathway for the production of muconic acid from a salicylic acid intermediate. The genetically engineered metabolic pathway comprises both biosynthetic and biodegradative elements.

Abstract: There is provided a microbial cell for producing at least one omega-functionalized carboxylic acid ester from at least one alkane, wherein the cell is genetically modified to increase the expression relative to the wild type cell of (i) Enzyme E1 capable of converting the alkane to the corresponding 1-alkanol; (ii) Enzyme E2 capable of converting the 1-alkanol of (i) to the corresponding 1-alkanal; (iii) Enzyme E3 capable of converting the 1-alkanal of (ii) to the corresponding alkanoic acid; (iv) Enzyme E4 capable of converting the alkanoic acid of (iii) to the corresponding alkanoic acid ester; and (iv) Enzyme E5 capable of converting the alkanoic acid ester of (iv) to the corresponding omega-hydroxy-alkanoic acid ester, and wherein the cell does not comprise a genetic modification that increases the expression relative to the wild type cell of at least one of the following enzymes E20-E24 selected from the group consisting of: E20 Acyl-ACP thioesterase, of EC 3.1.2.14 or EC 3.1.2.

Abstract: The present invention relates to oleaginous yeast strains overexpressing a hexokinase gene, wherein said strains are capable of accumulating lipids. Methods for obtaining said strains as well as methods for producing lipids are also disclosed.

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsatturated-saturated type.

Abstract: The present invention relates to a novel biocatalytic method for the production of primary and secondary amines, comprising coupled enzymatic oxidation and reduction processes simultaneously regenerating the required cofactor; making use of two enzymes—i.e. an alcohol dehydrogenase and an amine dehydrogenase—operating simultaneously in a one pot process (i.e. biocatalytic cascade). Furthermore, the overall process is redox neutral, since the hydride generated from the first oxidative step is internally recycled in the second reductive step. The invention also relates to recombinant expression systems and microorganisms procuring the required enzyme activities; and bioreactors for performing such methods.

Abstract: The present application relates to a microorganism of the genus Corynebacterium for producing L-lysine and a method for producing L-lysine by using the same. More specifically, the present application relates to: a recombinant microorganism of the genus Corynebacterium having increased L-lysine production, obtained by inactivating an intrinsic oxaloacetate decarboxylase of a Corynebacterium genus microorganism; and a method for producing L-lysine by using the same.

Abstract: The present invention relates to a modified polynucleotide encoding aspartate kinase (EC:2.7.2.4; hereinafter, referred to as LysC), transketolase (EC:2.2.1.1; hereinafter, referred to as Tkt) or pyruvate carboxylase (EC:6.4.1.1; hereinafter, referred to as Pyc), in which the initiation codon is substituted with ATG, a vector including the same, a microorganism transformed with the vector, and a method for producing L-lysine using the same.

Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.

Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pretreatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein the amounts of formed hydrolysed oxidation products at the end of the enzymatic hydrolysis by the oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 80 g/kg glucan present in the lignocellulosic material by adding a s

Abstract: The present disclosure relates to the field of organic chemistry in general. Particularly, the present invention relates to a process for conversion of biomass to simpler organic compound(s) such as carbohydrates. The process of the present disclosure provides for pre-treatment of biomass using acids organic acids and/or inorganic acids and further treatment using de-esterification agent, thereby rendering the biomass more amenable to enzymatic hydrolysis. The present disclosure also relates to application of the process in biofuel production by fermenting the organic products obtained by the process of the disclosure.

Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.

Abstract: A process for extracting carbohydrates from biomass and creating bioalcohol from the extracted carbohydrates. Subjecting the biomass to acid or alkali hydrolysis in a first hydrodynamic cavitation process. Filtering the first cavitated biomass to separate a first filtrate containing extracted carbohydrates. Fermenting the first filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. Subjecting the biomass to enzymatic hydrolysis in a second hydrodynamic cavitation process. Filtering the second cavitated biomass to separate a second filtrate containing extracted carbohydrates. Fermenting the second filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. The first and second filtrates may be combined and fermented in a single step.

Abstract: The present invention relates to the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-?-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-?-D-glucopyranose. Methods for producing the sugars are also provided.

Abstract: The invention relates to a method for production of single-stranded macronucleotides by amplifying and ligating an extended monomeric single-stranded target nucleic acid sequence (targetss) into a repetitive cluster of double-stranded target nucleic acid sequences (targetds), and subsequently cloning the construct into a vector (aptagene vector). The aptagene vector is transformed into host cells for replication of the aptagene and isolated in order to optain single-stranded target sequences (targetss). The invention also relates to single-stranded nucleic acids, produced by a method of the invention.

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5?-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.

Abstract: Genetically engineered host animal cells capable of producing glycoproteins having modified glycosylation patterns, e.g., defucosylation and/or monoglycosylation. Such host animal cells can be engineered to express fucosidase, endoglycosidase or both.

Abstract: The present invention relates to an antibody-like protein based on the tenth fibronectin type III domain (10Fn3) that binds to serum albumin. The invention further relates to fusion molecules comprising a serum albumin-binding 10Fn3 joined to a heterologous protein for use in diagnostic and therapeutic applications.

Abstract: Protein glycosylation greatly influences the structure, function, and pharmacokinetics of recombinant proteins. Here, growth media supplemented with metal ions is shown to modulate the protein glycosylation profile of recombinant proteins expressed in a variety of eukaryotic cell lines. In particular, millimolar amounts of ferric salts (e.g. ferric nitrate, ferric citrate, etc.) significantly increased the percentage of galactosylated N-glycan species. The effect on protein glycosylation was concentration-dependent manner up to 1.0 mM across multiple cell lines without adverse effects on cell viability. The use of iron supplements in cell culture media provides an efficient and effective approach towards the specific re-targeting of N-glycan glycoform profiles of recombinantly expressed proteins.

Abstract: The technical problem to be solved by the present invention is to provide a method for producing oxidized glutathione, GSSG and a precursor thereof, i.e., oxidized ?-glutamylcysteine, by a simple process. As a means for solving the problem, the method for producing GSSG according to the present invention comprises step A? of reacting L-cystine and L-glutamic acid to produce oxidized ?-glutamylcysteine and step B? of reacting oxidized ?-glutamylcysteine and glycine to produce GSSG.

Abstract: The invention provides a fungal cell, such as a yeast cell, capable of growing in co-cultivation with Propionibacterium. Also provided are methods of producing such cells and fermentation processes using the fungal cell of the invention and Propionibacterium in co-cultivation. Such co-cultivation significantly reduces the chemical oxygen demand load of the waste fermentation broth.

Abstract: A process for producing an optically active 2-alkyl-1,1,3-trialkoxycarbonylpropane (2), comprising a step of asymmetric hydrolysis of 2-alkyl-1,1,3-trialokoxycarbonylpropane (1) by using an enzyme capable of selectively hydrolyzing an ester moiety of either one enantiomer of 2-alkyl-1,1,3-trialkoxycarbonylpropane (1), or by using a culture of a microorganism capable of producing the enzyme or a treated object thereof.

Abstract: Provided is a method of screening for a microorganism having enhanced cellulose productivity.

Abstract: A specimen transfer apparatus includes control processing circuitry and a specimen support surface including a support base. The apparatus includes a lift mechanism to drive the specimen support surface and a drive mechanism to drive a drive rod rotatably connected to a drive motor for rotation about an axis. The apparatus includes a die cutter rotatably connected to the drive rod via support arms connected to the die cutter. The die cutter rotates from a first position to a second position about the axis of the drive rod. The specimen support surface includes a sensor array disposed in the specimen support surface. The processing circuitry is configured to detect a position of a first plate and a second plate via the sensor array. The lift and drive mechanisms are electrically connected to the processing circuitry. The processing circuitry is further configured to separately control the lift and drive mechanisms.

Abstract: In a first aspect, there is provided an isolated polypeptide substrate for a disintegrin-like and metallopeptidase with thrombospondin type-1 motif, 13 (ADAMTS13) that is from 45 to 70 amino acids in length and has an amino acid sequence that is substantially similar to part of the von Willebrand factor A2 domain sequence set forth in SEQ ID NO: 2, with one or more of the following modifications: (i) the amino acid corresponding to position 1599 of SEQ ID NO: 2 is mutated from Q to K; (ii) the amino acid corresponding to position 1610 of SEQ ID NO: 2 is mutated from N to C; and (iii) the amino acids corresponding to Q1624 to R1641 of SEQ ID NO: 2 are deleted.

Abstract: The present invention provides a method of detecting helicase activity in a test solution, providing a substrate solution containing a double-stranded DNA, wherein said double-stranded DNA is a substrate for a helicase to be detected; (b) introducing the substrate solution to the test solution to form a reaction solution; (c) applying a luminescent probe into the reaction solution to form a mixture; and (d) measuring a luminescent response of the luminescent probe in the mixture, wherein the luminescent response corresponds to the helicase activity in the test solution. The present invention also refers to a method of screening a potent luminescent probe for detecting helicase activity, and a series of luminescent Ir (III) complexes.

Abstract: The present invention relates to methods of imaging template hybridisation for estimating cluster numbers prior to solid phase amplification and sequencing. More particularly, an initial round of imaging is carried out at the single molecule template hybridisation stage which allows a general estimation of cluster numbers prior to clusters being formed. Amplification of the signal allows single molecule imaging to be carried out using standard sequencing imaging apparatus.

Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

Abstract: A screening method for the identification of a characteristic of a target nucleic acid in a whole blood sample, including positioning a composition comprising whole blood and at least one preservative agent within a centrifuge, centrifugating the composition to isolate a plasma that includes at least one target nucleic acid for further analysis and analyzing the at least one target nucleic acid to identify a characteristic about the at least one target nucleic acid, and a composition including the plasma, the preservative agent, and any other ingredient, which is produced by the method.

Abstract: The present disclosure provides methods, systems and compositions that provide transformable tagging moieties for use in analytical operations, and particularly in analysis of biological systems, such as in the analysis of gene expression in cell based systems.

Abstract: The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.

Abstract: The identification of genes upregulated following infection of citrus trees by Liberibacter, the causative agent of huanglongbing (HLB), is described. Methods for detecting pre-symptomatic HLB in citrus trees by detecting expression of one or more genes overexpressed following infection by Liberibacter is also described.

Abstract: A process for detecting Haemophilus influenzae nucleic acid in a sample includes producing an amplification product by amplifying a Haemophilus influenzae nucleotide sequence and measuring the amplification product to detect Haemophilus influenzae in the sample. Some embodiments allow direct serotype determination in a single step assay. Also provided are reagents and methods for detecting and distinguishing Haemophilus influenzae from other infectious agents. A kit is provided for detecting and quantifying Haemophilus influenzae in a sample.

Abstract: A process for detecting Haemophilus influenzae nucleic acid in a sample includes producing an amplification product by amplifying a Haemophilus influenzae nucleotide sequence and measuring the amplification product to detect Haemophilus influenzae in the sample. Some embodiments allow direct serotype determination in a single step assay. Also provided are reagents and methods for detecting and distinguishing Haemophilus influenzae from other infectious agents. A kit is provided for detecting and quantifying Haemophilus influenzae in a sample.

Abstract: A multiplexed nanopore sensing network comprising an integrated and multiplexed network of patch clamp capacitive integrator-differentiator amplifiers with small feedback capacitors using pseudo-resistors.

Abstract: The present invention is intended to provide a novel fluorescent labeled single-stranded nucleic acid, by which the background of an exciton oligomer can be further reduced and the novel use thereof. The present invention relates to a labeled single-stranded nucleic acid having at least two fluorescent atomic group pairs that exhibit an exciton effect. The labeled single-stranded nucleic acid is characterized in that the emission peak wavelength of one of the fluorescent atomic group pairs (fluorescent atomic group pair A) is shorter than the excitation peak wavelength of the other fluorescent atomic group pair (fluorescent atomic group pair B), and the fluorescent atomic group pairs A and B have a Förster resonance energy transfer (FRET) effect. This fluorescent labeled single-stranded nucleic acid is usable as a primer for amplifying a target nucleic acid or a probe to be hybridized with a target nucleic acid.

Abstract: The present invention relates to a method for forming nano-gaps in graphene. The method may include applying a voltage across a region of graphene such that a nano-gap which extends across the entire width of the graphene is formed, wherein the region across which the voltage is applied may include a point which is the narrowest in the region.

Abstract: The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

Abstract: The present invention relates generally to a method for assessing nucleic acid methylation, in particular DNA and methylation. More particularly, the present invention relates to a method of either qualitatively or quantitatively assessing, with improved sensitivity, the cytosine methylation of partially methylated DNA or RNA. The method of the present invention is useful in a range of applications including, but not limited to, the diagnosis of conditions or monitoring of developmental phenotypes which are characterised by DNA or RNA methylation changes.

Abstract: The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays.

Abstract: A detection method for detecting a detection target substance is provided. The method includes a first application step of applying a magnetic field a liquid suspension to link a plurality of magnetic particles in a row from an interior surface of a liquid receiving member that receives the liquid suspension of magnetic particles to which a detection target substance is bound, a second application step of bringing the linked magnetic particles near the interior surface while the magnetic field is applied to the liquid suspension, and a detection step of detecting the detection target substance bound to the magnetic particles brought near the interior surface by the second application step, wherein the direction of the magnetic field applied to the liquid suspension is altered so the linked magnetic particles approach the interior surface in the second application step.

Abstract: An article of manufacture having a plurality of sites in domains of regular patterns. Neighboring domains are oriented at different angles to improve the identification of the sites.

Abstract: The present invention provides devices and methods for detecting and capturing molecular biomarkers from a subject in situ. Specifically, the devices contain an array of microneedles to which are attached probes specific for one or more biomarkers of interest. The devices can be used directly on a subject (e.g., via skin piercing) in detecting the biomarkers in the body of the subject (e.g., tissues, blood stream).