Abstract: The present invention concerns a novel method and assembly for extraction of regenerative cellular components from an adipose tissue, the method comprising the steps of filtering a harvested adipose tissue for recovering its liquid fraction by means of a filtering element having a first cut-off value comprised between 40 microns and 70 microns; separating regenerative cellular components present in the liquid fraction by means of cell size segregation which blocks cells bigger than a second cut-off value ranging from 8 to 20 microns; and recovering the regenerative cells-rich fraction comprising the regenerative cellular components not having being blocked during the separating step.
Abstract: An object of the present invention is to provide a method for differentiating mesodermal cells into mesothelium cells. The present invention provides a method for producing mesothelial cells, including a first culturing step of culturing mesodermal cells in a medium containing basic fibroblast growth factor (bFGF) and oncostatin M.
Abstract: The present invention relates to organoids derived from a single cell, such as a prostate cancer cell, and methods and compositions relating to the production and use thereof, including cell culture medium for producing organoids and methods of personalized treatment for prostate cancer. The invention further provides a humanized mouse comprising a prostate organoid derived from a patient's prostate cell.
Type:
Application
Filed:
June 19, 2015
Publication date:
July 13, 2017
Applicant:
Rutgers, The State University of New Jersey
Abstract: As a technique capable of culturing anchorage-dependent cells without using an anchorage, provided is a medium for anchorage-dependent cells, which comprises MFG-E8 (Milk fat globule-EGF factor 8) or a fragment of the protein. This medium can promote adhesion of anchorage-dependent cells in the absence of an anchorage, enables the survival and proliferation (colony formation) of the cells, and further, also enables the subsequent differentiation if the anchorage-dependent cells are stem cells.
Abstract: The present disclosure relates to recombinant viral vectors, to pharmaceutical compositions comprising such recombinant vectors, and to methods for prevention and treatment of osteoarthritis in mammals. In particular, this disclosure provides adeno-associated virus (AAV) vectors capable of expressing, in a host, osteoprotective/chondroprotective bioactive proteins, including hyaluronan synthase 2 (HAS2) and lubricin (PRG4). Methods of production of these AAV are provided, as are methods of treatment of osteoarthritis in mammalian joints, by the long-term gene expression of osteoprotective/chondroprotective proteins, including HAS2 and PRG4, in both synovial and chondrocyte cells.
Type:
Application
Filed:
January 13, 2017
Publication date:
July 13, 2017
Applicants:
MERIAL INC., GENZYME CORPORATION
Inventors:
Monica Dias Figueiredo, Sirkka RM Kyostio-Moore, Patricia Berthelette
Abstract: The invention provides compositions and methods useful to prepare segmented, negative strand RNA viruses, e.g., orthomyxoviruses such as influenza A viruses, entirely from cloned cDNAs and in the absence of helper virus.
Abstract: Adeno-associated virus (AAV) Clade F vectors or AAV vector variants (relative to AAV9) for precise editing of the genome of a cell and methods and kits thereof are provided. Targeted genome editing using the AAV Clade F vectors or AAV vector variants provided herein occurred at frequencies that were shown to be 1,000 to 100,000 fold more efficient than has previously been reported. Also provided are methods of treating a disease or disorder in a subject by editing the genome of a cell of the subject via transducing the cell with an AAV Clade F vector or AAV vector variant as described herein and further transplanting the transduced cell into the subject to treat the disease or disorder of the subject. Also provided herein are methods of treating a disease or disorder in a subject by in vivo genome editing by directly administering the AAV Clade F vector or AAV vector variant as described herein to the subject.
Type:
Application
Filed:
January 23, 2017
Publication date:
July 13, 2017
Inventors:
Saswati CHATTERJEE, Laura Jane SMITH, Kamehameha WONG
Abstract: Compositions and transformation methods to increase the frequency of plants in a population of transformed plants which have a single copy of the target polynucleotide of interest are provided. The frequency of plants in a population of transformed plants containing no contaminating vector backbone sequence may be increased. The methods and compositions provide for a greater number of transgenic events having single copy inserts and no contaminating vector backbone sequence.
Type:
Application
Filed:
June 5, 2015
Publication date:
July 13, 2017
Applicant:
Pioneer HI Breed Internaional Inc.
Inventors:
Ajith Anand, Myeong-Je Cho, William Gordon-Kamm
Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
Abstract: Aspects of the disclosure relate to compositions and methods for site-directed DNA nicking and/or cleaving, and use thereof in, for example, polynucleotide assembly.
Type:
Application
Filed:
July 8, 2015
Publication date:
July 13, 2017
Inventors:
Joseph Jacobson, Ishtiaq Saaem, Michael E. Hudson, Devin Leake
Abstract: The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are compositions and methods related to components of a CRISPR complex particularly comprising a Cas ortholog enzyme.
Abstract: Compositions comprising glycosyl hydrolase enzymes are provided, as are methods for their use to depolymerize hemicellulose, cellulose, lignin and pectin in biomass in order to produce products such as simple sugars. The enzymes, isolated from Aspergillus nidulans and Phanerochaete chrysosporium, were characterized, and synergistic mixtures of the enzymes were produced and used to generate simple sugars from biomass without the need to pretreat the biomass before digestion. The enzyme blends generally comprise two or more enzymes, which may be from the same fungus or from two different fungi, and are used for efficient and cost effective complete degradation of lignocelluloses. Applications of this technology include biofuel production.
Type:
Application
Filed:
June 2, 2015
Publication date:
July 13, 2017
Applicant:
THE BOARD OF REGENTS FOR OKLAHOMA STATE UNIVERSITY
Inventors:
ANDREW MORT, ANAMIKA RAY, SAYALI S. SAYKHEDKAR, ROLF A. PRADE
Abstract: The present invention relates to a novel beta-galactosidase, and more particularly to a novel beta-galactosidase derived from Bacillus circulans, a gene encoding the beta-galactosidase, a recombinant vector and a recombinant microorganism, which contain the gene, a method for producing a beta-galactosidase using the recombinant microorganism, and a method for producing galactooligosaccharide using the beta-galactosidase. The use of the novel beta-galactosidase according to the present invention makes it possible to efficiently produce a large amount of galactooligosaccharide.
Type:
Application
Filed:
May 19, 2015
Publication date:
July 13, 2017
Inventors:
Jae Youl Choi, Jae Gu Pan, Seung Hwan Park, Eui Joong Kim
Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65° C. for 15 min. The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one amino acid substitution selected from the group consisting of P469A, P469C, P469D, P469E, P469F, P469G, P469H, P469I, P469K, P469L, P469M, P469N, P469Q, P469R, P469S, P469T, P469V, P469W, P469Y, a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.
Type:
Application
Filed:
June 3, 2015
Publication date:
July 13, 2017
Inventors:
Jan Metske VAN DER LAAN, Peter Jozef Ida VAN DE VONDERVOORT, Chantal CHRISTIS, Martine SPAANS, Angela DE BRUINE-PAULUS
Abstract: Disclosed are mammalian tau proteases, as well as proteolytically-active fragments, variants, and mutants thereof. Also disclosed are polynucleotides and recombinant expression vectors that encode these polypeptides, as well as methods for producing such proteins in selected recombinant host cells, and for using the compositions in a variety of diagnostic and analytical assays.
Type:
Application
Filed:
November 22, 2016
Publication date:
July 13, 2017
Inventors:
James G. Moe, Eliot J. Davidowitz, Patricia Lopez
Abstract: The present invention relates to novel human coagulation Factor VIIa variants having coagulant activity as well as polynucleotide constructs encoding such variants, vectors and host cells comprising and expressing the polynucleotide, pharmaceutical compositions, uses and methods of treatment.
Type:
Application
Filed:
February 24, 2017
Publication date:
July 13, 2017
Inventors:
Henrik Oestergaard, Ole Hvilsted Olsen, Katrine Skaarup Larsen, Henning Stennicke
Abstract: A method of creating a miniature multicellular biological construct and a method for studying cellular environments using the miniature multicellular biological construct is provided. The method for making the miniature multicellular biological construct includes suspending cells in a hydrogel, depositing the cell-suspension into a microwell, gelling the cell-suspension, and incubating the cell-suspension. The method for studying cellular environments includes imaging the miniature multicellular biological construct.
Abstract: The invention provides mutant Escherichia coli cells that contain one or more mutations in one or more of the rpoB, hns/tdk, cor A, ygaZ, iap, metL, ygeW, and pyrE/rph genes (exemplified in Table 2A and 2B), which confer on the mutant in M9-glucose minimal media the phenotype of increased level of growth and/or increased glucose uptake rate and/or increased acetate production rate and/or increased biomass yield, compared to a control E. coli (such as wild type E. coli) that lacks the one or more mutations in the one or more genes.
Abstract: The invention relates to the unexpected discovery of a system and methods for precise homology directed repair after CRISPR/Cas9 cleavage. The invention includes a DNA cleavage and repair system comprising a CRISPR/Cas9 system and an oligonucleotide 100% complementary to cleaved DNA to promote homology directed DNA repair. The invention further includes methods for inducing homology directed repair of cleaved DNA and repairing a CRISPR/Cas9 cleavage.
Type:
Application
Filed:
January 10, 2017
Publication date:
July 13, 2017
Inventors:
Eric Brian Kmiec, Pawel Alexander Bialk
Abstract: Provided herein are methods and compositions to extract and enrich by, physical separation or amplification, relatively short nucleic acids from a nucleic acid composition containing a high background of longer nucleic acids (e.g., host or maternal nucleic acids; genomic nucleic acid and the like).
Type:
Application
Filed:
January 18, 2017
Publication date:
July 13, 2017
Inventors:
Michele Elizabeth WISNIEWSKI, William Hang KWONG, Firouz MOHSENIAN, Jian-Hua DING
Abstract: A phenol-free method for isolating a nucleic acid from a sample is provided, said method comprising the following steps: a) preparing a precipitation mixture by adding at least one metal cation precipitant and at least one organic solvent selected from aprotic polar solvents and protic solvents to the sample, wherein the precipitation mixture i) comprises the metal cation precipitant; ii) comprises the organic solvent in a concentration of 15% or less; iii) comprises a buffering agent; and iv) has an acidic pH value, and precipitating proteins; b) separating the precipitate from the supernatant, wherein the supernatant comprises small RNA having a length of less than 200 nt and large RNA having a length of at least 1000 nt; and c) isolating a nucleic acid from the supernatant. Using an organic solvent as claimed during the protein precipitation step in the defined concentration provides a supernatant which in addition to small RNA also comprises large RNA.
Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.
Type:
Application
Filed:
July 9, 2015
Publication date:
July 13, 2017
Inventors:
Johan Karl Olov Skog, Daniel Enderle, Aparna Ramachandran, Haoheng Yan, Emily Berghoff, Tai-Fen Wei, Mikkel Noerholm
Abstract: The present invention relates to VNAR single chain antibodies and more particularly, to semi-synthetic VNAR libraries derived from nurse shark which may be used to identify individual clones, nucleic acid molecules and polypeptides which encode binding moieties that specifically bind to a cellular target of interest, thereby altering (e.g., antagonizing) target activity in a cell or mimicking the activity of a native molecule. The present invention thus also relates to compounds and compositions comprising a target specific VNAR binding moiety, methods for preparing them, and diagnostic and therapeutic methods of use relating to regulation, e.g., agonism or antagonism of the selected cellular target or target pathway e.g., to treat and/or prevent a pathological condition, disorder or disease in which it is beneficial to alter, e.g., agonize or augment, antagonize, reduce or eliminate the specific cellular target activity.
Abstract: Provided herein are, inter alia, methods for linking an mRNA molecule to a polypeptide (e.g., a peptide or a protein) by linking the mRNA molecule to a linking amino acid in the polypeptide, or by linking the mRNA molecule to a linking tRNA to which the polypeptide is attached, via reactions not catalyzed by the ribosome, and methods for making polypeptide libraries. Also provided are mRNA-protein complexes and mRNA-tRNA-protein complexes, libraries containing these complexes, and methods of using these complexes.
Abstract: The present invention relates to a method for synthesising templated molecules. In one aspect of the invention, the templated molecules are linked to the template which templated the synthesis thereof. The invention allows the generation of libraries which can be screened for e.g. therapeutic activity.
Type:
Application
Filed:
October 13, 2016
Publication date:
July 13, 2017
Inventors:
Henrik PEDERSEN, Alex Haahr GOUILAEV, Thomas FRANCH, Christian Klarner SAMS, Eva Kampmann OLSEN, Frank Abilgaard SLOK, Gitte Nystrup HUSEMOEN, Jakob FELDING, Lene HYLDTOFT, Mads NORREGAARD-MADSEN, Michael Anders GODSKESEN, Sanne Schroder GLAD, Thomas THISTED, Per-Ola FRESKGARD, Anette HOLTMANN
Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3? hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
Type:
Application
Filed:
March 24, 2017
Publication date:
July 13, 2017
Inventors:
Craig Betts, Steve Oh, George G. Jokhadze, Nathalie Bolduc
Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3? hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
Type:
Application
Filed:
March 24, 2017
Publication date:
July 13, 2017
Inventors:
Craig Betts, Steve Oh, George G. Jokhadze, Nathalie Bolduc
Abstract: A Type III-A CRISPR-Cas (StCsm) complex of Streptococcus thermophilus comprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed.
Type:
Application
Filed:
March 3, 2017
Publication date:
July 13, 2017
Inventors:
Virginijus Siksnys, Migle Kazlauskiene, Gintautas Tamulaitis
Abstract: Oligonucleotide analogues comprising modified intersubunit linkages and/or modified 3? and/or 5?-end groups are provided. The disclosed compounds are useful for the treatment of diseases where inhibition of protein expression or correction of aberrant mRNA splice products produces beneficial therapeutic effects.
Type:
Application
Filed:
August 25, 2016
Publication date:
July 13, 2017
Inventors:
Gunnar J. Hanson, Alexander Charles Rudolph, Bao Zhong Cai, Ming Zhou, Dwight D. Weller
Abstract: Methods for treating cancer, e.g., cancer of epithelial origin, by specifically targeting human satellite II (HSATII) using sequence specific agents such as oligonucleotides. As shown herein, the hetero chromatic HSATII satellite repeat is silenced in normal cells, but massively over expressed in epithelial cancers and in cancer cell lines when grown as xenografts or in 3D culture. Induction of HSATII RNA, either in xenografts or using in vitro reconstitution models, suggests the appearance of complementary DNA intermediates.
Type:
Application
Filed:
June 25, 2015
Publication date:
July 13, 2017
Inventors:
David T. Ting, Daniel A. Haber, Shyamala Maheswaran, Francesca Bersani, Anders M. Naar, Mihir Shivadatta Rajurkar
Abstract: Provided herein are compositions and methods for the modulation of miR-214 for the treatment and/or prevention of fibrosis and fibroproliferative conditions.
Abstract: Provided herein are compositions and methods for the inhibition of endothelial cell kinesin light chain 1, variant 1 (KLC1C) expression and/or activity, and treatment or prevention of inflammation therewith.
Abstract: The present invention relates to a composition for preventing or treating diseases associated with human papillomavirus (HPV), and more specifically, cancer associated with HPV, and even more specifically, cervical cancer. The nucleotide sequence of the present invention, the sequence in which the base thereof is modified, and a specific combination thereof can be useful in a composition for effectively treating diseases associated with HPV infection by greatly inhibiting the expression of the E6/E7 gene of HPV type 16 or 18.
Type:
Application
Filed:
February 23, 2017
Publication date:
July 13, 2017
Inventors:
Young Kee SHIN, Young Deug KIM, Hun Soon JUNG, Deuk Ae KIM
Abstract: Methods of modulating lung cancer, e.g., squamous cell carcinoma (SQCC), tumor initiating cells (TIC) are provided. Aspects of the methods including contacting a TIC with an OXTR modulatory agent, e.g., an inhibitory agent, in a manner sufficient to modulate the TIC. Aspects of the invention further include compositions that find use in practicing methods of the method. The methods and compositions find use in a variety of different applications, including but not limited to the treatment of SQCC.
Type:
Application
Filed:
July 6, 2015
Publication date:
July 13, 2017
Inventors:
Hua Fan-Minogue, Atul J. Butte, Jason Wheeler
Abstract: RNA interference is provided for inhibition of HIF1A mRNA expression for treating patients with ocular angiogenesis, particularly for treating retinal edema, diabetic retinopathy, sequela associated with retinal ischemia, posterior segment neovascularization (PSNV), and neovascular glaucoma, and for treating patients at risk of developing such conditions.
Abstract: Methods of treating movement disorders by reducing the activity of Nurr1 are disclosed. These methods are particularly applicable to subjects suffering from Parkinson's disease who have either developed levodopa-induced dyskinesia (LID) or are at risk of developing LID. In some aspects, the invention relates to a method for treating a movement disorder in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a composition, wherein said composition reduces the activity of a nuclea receptor related 1 protein (“Nurr1”). In some embodiments, the movement disorder is a dyskinesia. The movement disorder may be a levodopa-induced dyskinesia.
Type:
Application
Filed:
June 5, 2015
Publication date:
July 13, 2017
Inventors:
Fredic P. Manfredsson, Jack W. Lipton, Nicholas Kanaan, Timothy Collier, Kathy Steece-Collier, Caryl E. Sortwell
Abstract: A yeast terminator derived from a yeast DIT1 terminator when aligned with the nucleotide sequence represented by SEQ ID NO:1, includes a partial nucleotide sequence corresponding to the partial nucleotide sequence AGTTCG of positions 54 to 59 in the nucleotide sequence represented by SEQ ID NO:1, and also includes one or two or more mutations selected from the group made of (a) to (c): (a) a first mutation substituting TTTTTCT for the partial nucleotide sequence TTTTGTTCT of positions 27 to 35 in the nucleotide sequence represented by SEQ ID NO:1; (b) a second mutation substituting TCTTTT for the partial nucleotide sequence TCTCATTTT of positions 69 to 77 in the nucleotide sequence represented by SEQ ID NO:1; and (c) a third mutation substituting A for the G of position 51 in the nucleotide sequence represented by SEQ ID NO:1.
Abstract: The present invention relates to recombinant Gram-negative bacterial strains and the use thereof for delivery of heterologous proteins into eukaryotic cells.
Abstract: Compositions and methods for improving plant growth are provided herein. Polynucleotides, polypeptides, and expression constructs for expressing transcription factors (TFs) whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
Type:
Application
Filed:
July 8, 2015
Publication date:
July 13, 2017
Applicant:
Benson Hill Biosystems, Inc.
Inventors:
Thomas P. Brutnell, Benjamin N. Gray, Todd C. Mockler, Lin Wang
Abstract: This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode cytochrome P450 polypeptides from a Papaver somniferum cultivar; uses in the production of noscapine and identification of poppy cultivars that include genes that comprise said nucleic acid molecules.
Type:
Application
Filed:
March 27, 2017
Publication date:
July 13, 2017
Applicant:
Sun Pharmaceutical Industries (Australia) Pty Ltd
Inventors:
Thilo Hans Winzer, Tracy Carol Walker, Ian Alexander Graham
Abstract: The disclosure discloses isolated polynucleotides and polypeptides, and recombinant DNA constructs useful for conferring improved nitrogen use efficiency and drought tolerance; compositions (such as plants or seeds) comprising these recombinant DNA constructs; and methods utilizing these recombinant DNA constructs. The recombinant DNA constructs comprise a polynucleotide operably linked to a tissue specific or inducible promoter that is functional in a plant, wherein said polynucleotides encode NAC transcription factor polypeptides.
Type:
Application
Filed:
July 2, 2015
Publication date:
July 13, 2017
Inventors:
GUIHUA LU, YANG GAO, CONG LI, GUANFAN MAO, WEI WANG, XIPING WANG, CHANGGUI WANG
Abstract: The disclosure discloses isolated polynucleotides and polypeptides, and recombinant DNA constructs useful for conferring improved tolerance in plants to insect pests; compositions (such as plants or seeds) comprising these recombinant DNA constructs; and methods utilizing these recombinant DNA constructs. The recombinant DNA constructs comprise a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotides encode insect tolerance polypeptides.
Abstract: The present invention provides novel methods and systems, including cell lines, recombinant polynucleotide constructs, compositions, and kits, for targeted yet universal genomic manipulation.
Abstract: The systems and methods are directed to leveraging the channel geometry and configuration to overcome diffusion limitations of current transduction systems. The methods may include a method of transducing target cells using a device. The device may include at least one continuous channel. The method may include delivering target cells and viral vectors into a transduction region of the channel through a first inlet/outlet simultaneously and/or consecutively when one or more additional inlets/outlets that are located in a region of the channel downstream of the transduction are closed. After transducing for some incubation time, a flushing solution may be delivered through one or more of the additional inlets/outlets. The method may include collecting transduced cells after the transducing incubation time and the delivering of the flushing solution.
Type:
Application
Filed:
December 5, 2016
Publication date:
July 13, 2017
Inventors:
Reginald Tran, Wilbur Lam, David Myers, Christopher Doering, Harold Spencer
Abstract: The nucleic acid sequences of adeno-associated virus (AAV) serotype 1 are provided, as are vectors and host cells containing these sequences and functional fragments thereof. Also provided are methods of delivering genes via AAV-1 derived vectors.
Abstract: The present relation relates to recombinant vesicular stomatitis virus for use as prophylactic and therapeutic vaccines as well as the preparation and purification of immunogenic compositions which are formulated into the vaccines of the present invention.
Type:
Application
Filed:
January 27, 2017
Publication date:
July 13, 2017
Inventors:
Christopher L. Parks, Maoli Yuan, Kevin Wright, Christy Jurgens
Abstract: This invention relates to materials and methods for gene editing in mammalian cells, and more particularly to methods for gene editing using DNA-guided Argonaute (Ago) interference systems (DAIS) in T-cells.
Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more nucleic acid constructs fused with a pore-forming toxin, which may further be targeted to a particular cell type, wherein the nucleic acid constructs comprise an exogenous nucleic acid to be integrated into a genomic target site, and an enzyme, such as a nuclease or recombinase or a combination thereof capable of targeted introduction of the nucleic acid into the genome.
Type:
Application
Filed:
June 5, 2015
Publication date:
July 13, 2017
Applicants:
PRESIDENT AND FELLOWS OF HARVARD COLLEGE, MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Inventors:
Gerald MARSISCHKY, Bradley L. PENTELUTE, R. John Collier, Andrew J. MCCLUSKEY
Abstract: The present disclosure provides systems, compositions and methods for regulating expression of a target polynucleotide in a cell. The systems, compositions and methods comprise a chimeric receptor polypeptide comprising a G-protein coupled receptor (GPCR) or a fragment thereof, a chimeric adaptor polypeptide, at least one actuator moiety and a cleavage moiety.
Type:
Application
Filed:
January 10, 2017
Publication date:
July 13, 2017
Applicant:
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY