Abstract: The present invention relates to the use of micropeptides (peptides encoded by microRNAs or “miPEPs”) for promoting plant growth.

Abstract: The present invention provides plant virus vectors developed from the Foxtail mosaic virus (FoMV). The vectors include a nucleic acid sequence encoding an infectious Foxtail mosaic virus (FoMV) with a functional movement encoding sequence operably linked to one or more regulatory elements functional in a plant. The plant virus vectors may be used to infect monocot plants, such as maize and can be used for VIGS, gene editing, gene expression or other transgenic protocols.

Abstract: dsRNA generated from D. citri trehalase gene is effective in reducing fitness and/or survival of D. citri. Thus genetically altered plants expressing the dsRNA and plants to which dsRNA solutions are applied increase D. citri mortality and reduce D. citri infestation. With reduced D. citri population, the spread of microorganisms for which D. citri is a vector is reduced. Such microorganisms include, but are not limited to, C. Liberibacter species, including: CLas, CLam, and CLaf. Thus, applying of the D. citri trehalase dsRNA to a plant reduces disease and/or microorganism transmission by killing D. citri that feed on the treated plant.

Abstract: The present invention concerns methods and reagents useful in modulating gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to synthetic chemically modified small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules capable of mediating RNA interference (RNAi) against target nucleic acid sequences. The small nucleic acid molecules are useful in the treatment of any disease or condition that responds to modulation of gene expression or activity in a cell, tissue, or organism.

Abstract: Four genes, A622, NBB1, PMT, and QPT, can be influenced for increasing nicotinic alkaloid levels in Nicotiana plants, as well as for synthesizing nicotinic alkaloids in non-nicotine producing plants and cells. In particular, overexpressing one or more of A622, NBB1, PMT, and QPT may be used to increase nicotine and nicotinic alkaloid levels in tobacco plants. Non-nicotine producing cells can be engineered to produce nicotine and related compounds by overexpressing A622 and NBB1.

Abstract: Compositions and methods are provided for enhancing growth, seed germination, and biomass production in transgenic plants expressing reduced levels of GIGANTUS1 (gts1).

Abstract: Provided are methods for elevating cyclic electron transfer activity, improving carbon concentration, and enhancing carbon fixation in C3 and C4 plants, and algae, and producing biomass or other products from C3 or C4 plants, and algae, selected from among, for example, starches, oils, fatty acids, lipids, cellulose or other carbohydrates, alcohols, sugars, nutraceuticals, pharmaceuticals, fragrance and flavoring compounds, and organic acids, as well as transgenic plants produced thereby. These methods and transgenic plants and algae encompass the expression, or overexpression, of various combinations of genes that improve carbon concentrating systems in plants and algae, such as bicarbonate transport proteins, carbonic anhydrase, light driven proton pump, cyclic electron flow regulators, etc.

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP6 polypeptide.

Abstract: A novel class of transporter protein, referred to as SWEET, GLUE or Glü, is disclosed. These transporters provide a novel system for the transportation of sugars across membranes within a cell and between the inside and outside of a cell. Such transporters are useful for understanding and altering the sugar concentration within certain organs of an organism, and within certain organelles within the cell. These transporters are also useful in protecting plants from a pathogen attack.

Abstract: A Fusarium head blight (FHB) resistant gene Tafhb1 of wheat and uses thereof are disclosed, in which the FHB resistant gene Tafhb1 of wheat has a cDNA sequence as shown in SEQ ID NO. 1. A protein TaFHB1 encoded by the FHB resistant gene Tafhb1 of wheat has an amino acid sequence as shown in SEQ ID NO. 2. The protein includes 274 amino acids, and has an isoelectric point of 10.85. The FHB resistant gene Tafhb1 of wheat is transferred to wheat by crossing and multiple generations of backcrossing, to increase the resistance of wheat to FHB. Because the gene is an endogenous gene existing in the cereal crop wheat, the presence of the gene has no influence on the food safety of plants, such that the gene can be used in crop breeding.

Abstract: Provided herein are methods and compositions for inducing a somatic cell to acquire a less differentiated phenotype and for generating induced pluripotent stem cells (i PS cells) by inducing expression of ASF1A in the cell and/or by contacting the cell with GDF9. Also provided herein are compositions and methods for treating and/or diagnosing cancer and for identifying agents useful in the treatment and/or diagnosis of cancer.

Abstract: Novel adeno-associated virus (AAV) vectors in nucleotide and amino acid forms and uses thereof are provided. The isolates show specific tropism for certain target tissues, such as blood stem cells, liver, heart and joint tissue, and may be used to transduce stem cells for introduction of genes of interest into the target tissues. Certain of the vectors are able to cross tightly controlled biological junctions, such as the blood-brain barrier, which open up additional novel uses and target organs for the vectors, providing for additional methods of gene therapy and drug delivery.

Abstract: Novel adeno-associated virus (AAV) vectors in nucleotide and amino acid forms and uses thereof are provided. The isolates show specific tropism for certain target tissues, such as blood stem cells, liver, heart and joint tissue, and may be used to transduce stem cells for introduction of genes of interest into the target tissues. Certain of the vectors are able to cross tightly controlled biological junctions, such as the blood-brain barrier, which open up additional novel uses and target organs for the vectors, providing for additional methods of gene therapy and drug delivery.

Abstract: Novel adeno-associated virus (AAV) vectors in nucleotide and amino acid forms and uses thereof are provided. The isolates show specific tropism for certain target tissues, such as blood stem cells, liver, heart and joint tissue, and may be used to transduce stem cells for introduction of genes of interest into the target tissues. Certain of the vectors are able to cross tightly controlled biological junctions, such as the blood-brain barrier, which open up additional novel uses and target organs for the vectors, providing for additional methods of gene therapy and drug delivery.

Abstract: Novel adeno-associated virus (AAV) vectors in nucleotide and amino acid forms and uses thereof are provided. The isolates show specific tropism for certain target tissues, such as blood stem cells, liver, heart and joint tissue, and may be used to transduce stem cells for introduction of genes of interest into the target tissues. Certain of the vectors are able to cross tightly controlled biological junctions, such as the blood-brain barrier, which open up additional novel uses and target organs for the vectors, providing for additional methods of gene therapy and drug delivery.

Abstract: The invention relates to recombinant VSV viruses and viral vectors which produce a glycoprotein GP of the lymphocyte choriomeningitis virus (LCMV) instead of the G protein of the VSV, to virus producing cells which produce LCMV-GP-pseudotyped VSV virions, and to the use of said vectors and cells in the therapy of solid tumors, especially brain tumors.

Abstract: The invention provides an adenovirus or adenoviral vector characterized by comprising one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pIX protein, DNA polymerase protein, penton protein, hexon protein, and/or fiber protein.

Abstract: The invention relates to vectors comprising two or more homologous nucleotide sequences and methods for generating them. The invention concerns substituting bases in the homologous nucleotide sequences with different bases that do not alter the encoded amino acid sequence. The invention allows for the reduction of intramolecular recombination between homologous nucleotide sequences, in particular in mammalian cells. The invention further relates to nucleotide sequences containing substituted bases.

Abstract: Methods and compositions are provided for modifying one or more target loci in a cell. Such methods comprise providing a cell comprising a first polynucleotide encoding a first selection marker operably linked to a first promoter active in the cell, wherein the first polynucleotide further comprises a first recognition site for a first nuclease agent. A first nuclease agent is introduced into a cell, wherein the first nuclease agent induces a nick or double-strand break at the first recognition site. Further introduced into the cell is a first targeting vector comprising a first insert polynucleotide flanked by a first and a second homology arm that correspond to a first and a second target site located in sufficient proximity to the first recognition site. At least one cell is then identified comprising in its genome the first insert polynucleotide integrated at the target locus.

Abstract: The present invention relates to a recombinant methanotroph having an ability to produce isoprene and a method for producing isoprene using the same, and more particularly to a recombinant methanotroph having an ability to produce isoprene wherein a gene encoding an isoprene synthase having a homology of at least 70% to the amino acid sequence of Ipomoea batatas isoprene synthase is introduced into the recombinant methanotroph, and a method for producing isoprene using the recombinant methanotroph. The use of a recombinant methanotroph according to the present invention enables isoprene to be produced in high yield by using methane gas or methanol which is obtained from waste such as natural gas, biomass, municipal waste or the like as a carbon source.

Abstract: The Rhodosporidium toruloides IPM33-18 strain (NITE BP-01900) or a genetically modified variant thereof is provided as a novel yeast that is useful for producing oils from biomass. Further, a method for producing oils, comprising the steps of culturing the IPM33-18 strain or the like in a medium containing a carbon source to allow oils to accumulate in a culture, and collecting the oils from the culture is provided. According to this production method, the oils can be collected from a liquid fraction of the culture.

Abstract: The present disclosure provides compositions and methods for biologically producing very long carbon chain compounds (longer than C24), such as fatty acyl-CoA, fatty aldehydes, fatty alcohols, fatty ester waxes, alkanes and ketones, from recombinant C1 metabolizing microorganisms that utilize C1 substrates, such as methane or natural gas as a feedstock.

Abstract: The present disclosure provides compositions and methods for the biosynthetic production of choline, ethanolamine, phosphoethanolamine, and phosphocholine.

Abstract: The present disclosure provides compositions and methods for the biosynthetic production of acetaminophen, p-aminophenol, and p-aminobenzoic acid and the purification of biologically derived acetaminophen.

Abstract: The present disclosure provides compositions and methods for the biosynthetic production of carnosine and beta-alanine.

Abstract: The present invention relates to a recombinant nucleic acid molecule, a recombinant microorganism, to a method for producing alanine and to the use of the recombinant nucleic acid molecule or the recombinant microorganism for the fermentative production of alanine.

Abstract: The present disclosure relates to an improved method for producing ergothioneine, comprising the steps of: (a) inoculating Pleurotus ostreatus strain CGMCC No. 6232 into a seed medium, and culturing it to prepare a seed liquor, wherein the seed medium uses soybean cake powder as nitrogen source; and (b) inoculating the seed liquor into a fermentation basal medium, and then culturing it to obtain a fermentation broth of Pleurotus ostreatus mycelia. Further, any one or more members selected from NH4Cl, NH4NO3, NaCl, polyethylene glycol, folic acid, vitamin B1 (VB1), indolebutyric acid, citric acid, pyruvic acid, arginine, lysine, leucine, aspartic acid, glutamic acid, betaine, histidine, cysteine, methionine, tween, span, chitosan, Fluconazole, Miconazole, Ketoconazole, ethylenediaminetetraacetic acid (EDTA), isopropyl alcohol and dimethyl sulfoxide are added into the fermentation basal medium.

Abstract: A method for producing a partially hydrolysed lignocellulosic material is provided including treating a lignocellulosic material with an acid and/or an alkali and then a polyol. Also provided are methods of producing a fermentable sugar, or a fermentable sugar and a fermentation product from said partially hydrolysed lignocellulosic material. A partially hydrolysed lignocellulosic material, a fermentable sugar, and fermentation product produced by such methods are also provided. Also provided is an apparatus for producing a partially hydrolysed lignocellulosic material, such as by the aforementioned method.

Abstract: A novel the Ensifer adhaerens strain isolated from soil and a method of producing psicose using the same are provided.

Abstract: A photosynthetic device includes a main body defining a microfluid chamber for causing photosynthesis therein, and a light source, wherein the microfluid chamber is constituted by at least one communication room, a plurality of micro channels respectively and spatially communicated with the communication room, at least one micro injection duct spatially communicated with the communication room, and a plurality of filter plugs spatially connected to the micro channels respectively and the micro injection duct at free ends thereof in order to filter fluid backflow in the micro channels and the micro injection duct. The light source radiates ceaselessly one of the communication room, the plurality of micro channels and the micro injection duct. The photosynthesis is resulted once chloroplasts and normal saline solution are injected into one of the communication room, the plurality of micro channels and the micro injection duct.

Abstract: The invention concerns a method for producing, on an industrial scale, capsular polysaccharide of Haemophilus influenzae type b (PRP) intended for vaccine purposes, according to which a strain of Haemophilus influenzae type b (Hib) is cultured in a culture medium, the culture supernatant is harvested and treated in order to extract the capsular polysaccharide therefrom, said culture medium comprising at least: —one source of carbon, —protoporphyrin, —salts, —amino acids, —NAD or NADH, —vitamins, —means for regulating the pH, characterised in that said culture medium is chemically defined.

Abstract: Provided is a method of preparing a high-purity galactooligosaccharide composition by fermentation of a low-purity galactooligosaccharide mixture with a yeast strain, Kluyveromyces lactis ATCC 8585. The high-purity galactooligosaccharide composition includes at least 99% of galactooligosaccharides selected from the group consisting of galactotriose, galactotetrose, galactooligosaccharides with five or more sugar units, and combinations thereof and lower than 1% of monosaccharides and disaccharides. Also provided is a high-purity galactooligosaccharide composition and applications thereof in regulating blood glucose level and improving gut microbiota. For example, the high-purity galactooligosaccharide composition may be used to manufacture food products, beverages, health food products, nutritional supplements, and pharmaceutical compositions for patients or pets afflicted with diabetes mellitus or lactose intolerance.

Abstract: Provided is a method for preparing Rebaudioside M by using an enzyme method. In the method, Rebaudioside A or Rebaudioside D is used as a substrate, and in the presence of sucrose and UDP, Rebaudioside M is generated by means of reaction of the substrate under the catalysis of a mixture of UDP-glucosyl transferase and sucrose synthetase, or recombinant cells containing the UDP-glucosyl transferase and sucrose synthetase. The reaction is carried out in an aqueous-phase system at a temperature of 20 to 60° C. and pH 5.0 to 9.0. The reaction system further contains dimethyl sulfoxide at a concentration of 3% to 5% according to the ratio by volume for facilitating solubilization of the substrate.

Abstract: Polypeptide preparations having target levels of glycans, and methods of producing such polypeptide preparations using DMSO, are described.

Abstract: An isolated nucleic acid encoding an FX protein having a serine at position 79, a lysine at position 90, a leucine at position 136, an arginine at position 211, a serine at position 289, and a combination thereof is provided. Cells having a gene encoding a modified FX protein are provided, wherein the cells exhibit a reduced ability to fucosylate a glycoprotein at a first temperature, but exhibit the ability to fucosylate the glycoprotein at a second temperature. Methods and compositions for making glycoproteins with reduced fucosylation are provided.

Abstract: The present invention provides a method for producing a recombinant protein, which can increase the cell growth rate in culture while stably maintaining plasmids in the cells, thereby improving the recombinant protein yield. The present invention provides a method for producing a recombinant protein, including a high-temperature culture step of culturing at 32° C. or higher a Brevibacillus bacterium that contains a gene encoding a recombinant protein, and a low-temperature culture step of culturing the Brevibacillus bacterium at a temperature lower than 32° C. after the high-temperature culture step.

Abstract: The technical problem to be solved by the present invention is to provide a method for producing glutathione and a precursor thereof, ?-glutamylcysteine, at a high yield. As a means for solving the problem, the method for producing glutathione according to the present invention includes step A? of reacting L-cysteine and L-glutamic acid under a low-oxygen atmosphere to produce ?-glutamylcysteine and step B? of reacting ?-glutamylcysteine and glycine under a low-oxygen atmosphere to produce glutathione.

Abstract: Disclosed is a host cell for producing a recombinant peptide, polypeptide or protein of interest, wherein the host cell includes at least 2 copies of a nucleic acid sequence encoding a poison protein; and to the use thereof for producing peptides, polypeptides or proteins of interest.

Abstract: The present invention relates to a method for producing a carotenoid-protein complex in vivo comprising the steps of transforming of prokaryote cell with genes involved in carotenoid synthesis and with a gene encoding an apo-carotenoprotein; culturing said prokaryote cells such as to induce sequential genes expression, isolating and purifying the carotenoid-protein complex.

Abstract: Provided herein are methods for rapidly identifying a microbe, such as a pathogen, from a biological sample including, blood, urine, wound effluent, stool, serum, and bronchoalveolar lavage fluid. The method comprises obtaining the sample from the subject and performing a spectrometric analysis of the lipids in the microbe to obtain a profile. The profile obtained is compared with a molecular mass lipid profile of known microbes for identification. Also provided is a method for identifying one or more antimicrobial drugs effective to treat a microbial strain in a subject.

Abstract: The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.

Abstract: This invention relates to a capacitor comprising two electrical conductors separated by a dielectric, the dielectric comprising microorganisms. The dielectric may comprise a biological indicator. This invention relates to a process for determining whether the microorganisms are alive or dead. The number of microorganisms can be determined. This invention relates to a process for testing the efficacy of a sterilization process using the capacitor.

Abstract: A method of preparing a characterization zone of an analysis plate to carry out a characterization of a population of a microorganism in the presence of an antimicrobial agent by mass spectrometry using the MALDI technique. The method involves the following steps in succession: a step for providing an analysis plate for a characterization by means of the MALDI technique, the plate including an analysis zone carrying an antimicrobial agent; a step of depositing the population of a microorganism onto said analysis zone in contact with the antimicrobial agent; an incubation step of preserving the analysis plate under conditions and for a sufficient time to allow the antimicrobial agent and the microorganism that is present to interact; and a step of depositing a matrix that is suitable for the MALDI technique onto the analysis zone; associated characterization and functionalization methods, analysis plates and uses.

Abstract: The disclosure provides methods for detecting the concurrent presence of at least two targets within a biological sample. The method includes contacting said biological sample with a first binding agent, said first binding agent operably linked to a first sortase molecule, wherein said first binding agent specifically binds to a first target; contacting said biological sample with a second binding agent, said second binding agent operably linked to a first sortase recognition sequence peptide, wherein said second binding agent specifically binds to a second target; adding a sortase substrate under conditions where a first sortase-mediated ligation of the sortase substrate to the first sortase recognition sequence will produce a ligation product, and detecting the ligation product wherein detection of said ligation product indicates the concurrent presence of the first target and the second target in the biological sample. Also disclosed are kits comprising reagents for performing the methods as claimed.

Abstract: A device is disclosed for conducting a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid. An indicator formulation of the device changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A carrier substrate of the device is constructed of a material having voids providing a high void volume within the substrate. The device is made by applying a chromagen to the carrier substrate to create a chromagen-laden carrier member. Then, a selected reagent having a particular constituent-specific formulation is applied to the chromagen-laden member. The selected reagent then combines with the chromagen thereby establishing the indicator formulation within the carrier substrate in place for reception of a sample of the bodily fluid.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: The present invention pertains to an in vitro method in which a targeted DNA molecule containing a DNA sequence of interest is enriched by a) general amplification of DNA molecules in a multiple of droplets each containing less than 0.5 target DNA molecule on average (404), b) specific detection of the target DNA molecule in each of the droplets (405), and c) physically selecting droplets containing target DNA molecules (406).

Abstract: Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.

Abstract: The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.