Patents Issued in August 3, 2017
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Publication number: 20170218376Abstract: In certain aspects, provided herein are RNA complexes (e.g., asymmetric RNA complexes, such as asiRNAs or cell penetrating asiRNAs) that inhibit IL4R?, TRPA1, and/or F2RL1 expression and are therefore useful for treating atopic dermatitis or asthma.Type: ApplicationFiled: February 1, 2017Publication date: August 3, 2017Inventors: Dong-Ki Lee, Sun Woo Hong, Hanna Lee, Dayeon Yu, Ji Eom
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Publication number: 20170218377Abstract: The present invention relates to recombinant Trichoderma host cells producing Aspergillus fumigatus cellulolytic enzyme compositions and methods of producing and using the compositions.Type: ApplicationFiled: April 17, 2017Publication date: August 3, 2017Applicant: Novozymes, Inc.Inventors: Jeffrey Shasky, Brett McBrayer
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Publication number: 20170218378Abstract: The present invention relates to compositions and methods for delivering lysosomal proteins. The compositions and methods described herein permit the targeted delivery of exogenous lysosomal proteins to cell surface proteins that allow their internalization via non-clathrin pathways. The present invention further relates to the use of the compositions and methods for enzyme replacement therapy of lysosomal storage diseases. Nucleic acids, recombinant cells and kits useful for making and using the compositions of the invention are also provided.Type: ApplicationFiled: April 18, 2017Publication date: August 3, 2017Inventors: Silvia MURO GALINDO, Vladimir R. MUZYKANTOV, Edward Howard SCHUCHMAN
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Publication number: 20170218379Abstract: A self-regulating gene expression construct comprises a single promoter in operative association with a repressor sequence (e.g., bacterial repressor lacI or gaiR), operator sequence(s) responsive to the expressed repressor protein, and a transgene. A dual-regulating construct comprises a single promoter controlling expression of a bacterial repressor sequence and a transgene, and which, in the presence of a first inducer molecule, transcribes the transgene and repressor; and a ribozyme in association with an aptamer sequence, the aptamer sequence capable of interacting with a second inducer molecule to terminate mRNA degradation by the ribozyme. Also provided are recombinant vectors or viruses containing the self-regulating or dual self-regulating constructs and cells containing the vectors. Such compositions are useful in methods of treating a diseases using gene therapy.Type: ApplicationFiled: July 31, 2015Publication date: August 3, 2017Inventors: Mitchell LEWIS, Jean BENNETT, Luk VANDENBERGHE, Matthew SOCHOR, Theodore G. DRIVAS
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Publication number: 20170218380Abstract: Described herein are techniques for directional cloning an insert DNA segments into a target vector. The techniques mix the target vector, the insert DNA segment, a restriction enzyme, and a DNA ligase to generate a recombinant DNA molecule.Type: ApplicationFiled: April 7, 2017Publication date: August 3, 2017Inventors: Lei Xiao, Zhao Wu, Mao Bi
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Publication number: 20170218381Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; resulting cells, and methods for producing a polypeptide of interest using the resulting cells.Type: ApplicationFiled: April 7, 2017Publication date: August 3, 2017Applicant: Novozymes A/SInventors: Anne Breuner, Michael Dolberg Rasmussen
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Publication number: 20170218382Abstract: An expression vector is disclosed which contains a promoter DNA; a DNA encoding a peptide having a defined amino acid sequence and having secretion signal activity; and a DNA encoding an intended protein or a cloning site for insertion of the DNA encoding an intended protein. An expression vector is also disclosed which contains a promoter DNA; a DNA encoding any peptide having a defined amino acid sequence and having secretion signal activity; a DNA encoding an intended protein or a cloning site for insertion of the DNA encoding an intended protein; and a DNA encoding an anchor domain. The peptide having secretion signal activity allows for secretory production and cell surface display of a protein with high activity, in yeast.Type: ApplicationFiled: July 30, 2015Publication date: August 3, 2017Applicant: National University Corporation Kobe UniversityInventors: Akihiko KONDO, Tomohisa HASUNUMA, Kentaro INOKUMA
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Publication number: 20170218383Abstract: Transcription factor polynucleotides and polypeptides incorporated into nucleic acid constructs, including expression vectors, have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these constructs have been shown to have increased tolerance to an abiotic stress (in some cases, to more than one abiotic stress), increased growth, and/or increased biomass. The abiotic stress may include, for example, salt, hyperosmotic stress, water deficit, heat, cold, drought, and/or low nutrient conditions.Type: ApplicationFiled: April 14, 2017Publication date: August 3, 2017Inventors: T. Lynne Reuber, Oliver J. Ratcliffe, Frederick D. Hempel, Luc J. Adam, Cai-Zhong Jiang, Robert A. Creelman, Jose Luis Riechmann, Jacqueline E. Heard, Raymond R. Samaha, Pierre E. Broun, Magnus Hertzberg, Torgny Nasholm
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Publication number: 20170218384Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for a Setaria italica (Foxtail Millet) (SI-Ubiquitin) and Sorghum bicolor (SB-Ubiquitin) regulatory regions. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.Type: ApplicationFiled: August 4, 2015Publication date: August 3, 2017Inventors: SHANE ABBITT, MICHELLE VAN ALLEN
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Publication number: 20170218385Abstract: Various embodiments are directed to transgenic plants, including transgenic tobacco plants and derivative seeds, genetically modified to impede the transport of Cadmium (Cd) from the root system to aerial portions of transgenic plants by reducing the expression levels of HMA-related transporters. Various embodiments are directed to transgenic tobacco plants genetically modified to stably express a RNAi construct encoding RNAi polynucleotides that enable the degradation of endogenous NtHMA RNA variants. Reduced expression of NtHMA transporters in transgenic plants results in substantially reduced content of Cadmium (Cd) in the leaf lamina. Various consumable products that are substantially free or substantially reduced in Cd content can be produced by incorporating leaves derived from transgenic tobacco plants modified to reduce the expression of NtHMA transporters.Type: ApplicationFiled: April 12, 2017Publication date: August 3, 2017Applicant: Philip Morris USA Inc.Inventors: Alec J Hayes, Chengalrayan Kudithipudi, Rutger S van der Hoeven
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Publication number: 20170218386Abstract: The invention relates to genetically modified agricultural plants with increased oil content in vegetative tissues, as well as to expression systems, plant cells, seeds and vegetative tissues related thereto.Type: ApplicationFiled: April 13, 2017Publication date: August 3, 2017Inventors: Christoph Benning, Sanjaya
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Publication number: 20170218387Abstract: Compositions and methods for improving plant growth are provided herein. Compositions comprise promoter sequences that direct expression of an operably linked nucleotide in a developmentally regulated manner. Polynucleotides, polypeptides, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.Type: ApplicationFiled: July 23, 2016Publication date: August 3, 2017Applicant: Benson Hill Biosystems, Inc.Inventors: Thomas P. Brutnell, Douglas W. Bryant, Todd Christopher Mockler, Lin Wang
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Publication number: 20170218388Abstract: A method of improving abiotic stress tolerance of a plant is provided. The method comprising genetically modifying the plant to express miRNA167 in an abiotic stress responsive manner, wherein a level of expression of total miR167 under the abiotic stress conditions is selected not exceeding 10 fold compared to same in the plant when grown under optimal conditions, thereby improving abiotic stress tolerance of the plant.Type: ApplicationFiled: April 13, 2017Publication date: August 3, 2017Applicant: A.B. Seeds Ltd.Inventors: Orly NOIVIRT-BRIK, Rudy MAOR, Amir AVNIEL
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Publication number: 20170218389Abstract: The present disclosure provides methods for regulating stomata in plants, improving drought tolerance, and increasing resistance to bacterial pathogens through overexpression of genes NHR1 or GCN4. Also provided are transgenic plants with improved drought tolerance and increased resistance to bacterial pathogens produced by such methods.Type: ApplicationFiled: January 11, 2017Publication date: August 3, 2017Inventors: Kirankumar Mysore, Amita Kaundal, Seonghee Lee
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Publication number: 20170218390Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.Type: ApplicationFiled: January 31, 2017Publication date: August 3, 2017Inventors: Kenneth E. Narva, Sarah Worden, Meghan Frey, Murugesan Rangasamy, Premchand Gandra, Wendy Lo, Elane Fishilevich, Rainer Fischer, Andreas Vilcinskas, Eileen Knorr
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Publication number: 20170218391Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.Type: ApplicationFiled: January 31, 2017Publication date: August 3, 2017Inventors: Kenneth E. Narva, Sarah Worden, Meghan Frey, Murugesan Rangasamy, Premchand Gandra, Wendy Lo, Elane Fishilevich, Rainer Fischer, Andreas Vilcinskas, Eileen Knorr
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Publication number: 20170218392Abstract: The present invention relates to a new gene fragment derived from Chinese hamster ovary (CHO) cell for enhancement of recombinant protein expression in animal cells and a use thereof. It has been found that using the vector comprising a gene fragment of the present invention enhances the expression of a target protein in animal cells. Accordingly, the vector comprising a gene fragment of the present invention could be usefully used in the production of biopharmaceuticals such as therapeutic antibodies, etc.Type: ApplicationFiled: June 16, 2015Publication date: August 3, 2017Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Eun Gyo LEE, Hong-Weon LEE, Shin Young KANG, Yeon-Gu KIM, Joon Ki JUNG, Jungoh AHN, Seung Hee KANG, Chun Sug KIM, Hyeok Won LEE, Jin Gyeom LEE
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Publication number: 20170218393Abstract: The present invention discloses methods and compositions for modulating the quality of an immune response to a target antigen in a mammal, which response results from the expression of a polynucleotide that encodes at least a portion of the target antigen, wherein the quality is modulated by replacing at least one codon of the polynucleotide with a synonymous codon that has a higher or lower preference of usage by the mammal to confer the immune response than the codon it replaces.Type: ApplicationFiled: January 17, 2017Publication date: August 3, 2017Inventors: Ian Hector Frazer, Julia Louise Dutton
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Publication number: 20170218394Abstract: Methods of producing hepatocyte lineage cells are provided. The method can include transfecting a cell with one or more expression vectors. For example, a cell can be transfected with a first expression vector containing a first gene that encodes CCAAT/enhancer binding protein alpha (CEBPA), a second expression vector containing a second gene that encodes CCAAT/enhancer binding protein beta (CEBPB), a third expression vector containing a third gene that encodes forkhead box A1 (FOXA1), and a fourth expression vector containing a fourth gene that encodes forkhead box A3 (FOXA3). The method can include culturing the transfected cell obtained in a growth environment. The transfected cell can be cultured in Williams' E medium, ReproFF (feeder-free media maintaining pluripotency) medium, or both. Transfected and/or hepatocyte lineage cells obtained by a method of the present invention are also provided.Type: ApplicationFiled: January 27, 2017Publication date: August 3, 2017Applicant: National University Corporation Chiba UniversityInventor: Minoru Tomizawa
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Publication number: 20170218395Abstract: Provided herein are methods related to co-packaging of multiple rAAV particles, e.g., by introducing multiple nucleic acid vectors encoding proteins or polypeptides or RNAs of interest into a single cell preparation.Type: ApplicationFiled: June 20, 2015Publication date: August 3, 2017Applicant: University of Florida Research Foundation, Inc.Inventors: Barry John Byrne, Phillip A. Doerfer, Nathalie Clement
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Publication number: 20170218396Abstract: A cell modified for obtaining increased proliferative capacity, decreased aging and enhanced regenerative capacity, its modification involving HOXB7 overexpression obtained using a gene vector that can insert the coding sequence into the cell, thereby affording increased protein production.Type: ApplicationFiled: July 30, 2015Publication date: August 3, 2017Inventors: Massimo Dominici, Olivia Candini
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Publication number: 20170218397Abstract: A method, system, and apparatus for treating a patient with HIV. A vector can be modified from a thymidine kinase gene. The modified vector is expressed in the presence of tat RNA. The modified vector is then package and delivered to HIV-infected cells. The replication of HIV is inhibited by eliminating infected cells in the presence of Ganciclovir. Modified cells are then selected utilizing transient tat RNA transfection and GFP expression. Vector-modified stem cells are then selected for transplantation back into the patient, thereby producing a normal immune system in the patient when the modified vector remains dormant in the absence of HIV tat.Type: ApplicationFiled: July 20, 2015Publication date: August 3, 2017Inventors: Himanshu Garg, Anjali Joshi
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Publication number: 20170218398Abstract: Methods and compositions for selectively targeting cancerous cells for genetic manipulation based on cancer specific sequence motifs (CSSMs), which are formed as a result of chromosomal rearrangement, and cells produced by said methods.Type: ApplicationFiled: January 30, 2017Publication date: August 3, 2017Inventors: Markus Alexander Brown, Reinhard Ebner
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Publication number: 20170218399Abstract: Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.Type: ApplicationFiled: April 12, 2017Publication date: August 3, 2017Inventors: Wojtek Auerbach, Thomas DeChiara, William Poueymirou, David Frendewey, David Valenzuela
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Publication number: 20170218400Abstract: The present invention relates in part to nucleic acids encoding proteins, therapeutics comprising nucleic acids encoding proteins, methods for inducing cells to express proteins using nucleic acids, methods, kits and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods and products for altering the DNA sequence of a cell are described, as are methods and products for inducing cells to express proteins using synthetic RNA molecules. Therapeutics comprising nucleic acids encoding gene-editing proteins are also described.Type: ApplicationFiled: April 13, 2017Publication date: August 3, 2017Inventors: Matthew Angel, Christopher Rohde
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Publication number: 20170218401Abstract: Provided are genetically engineered microorganism that catalyze the synthesis of propane and/or butanol from a suitable substrate such as glucose. Also provided are methods of engineering said genetically engineered microorganism and methods of producing propane and/or butanol using the genetically engineered microorganism.Type: ApplicationFiled: February 1, 2017Publication date: August 3, 2017Inventors: Nigel Scrutton, Patrik Jones, Navya Menon
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Publication number: 20170218402Abstract: Methods of identifying genes conferring ethanol tolerance in yeasts, genes that confer ethanol tolerance, and mutant strains used to identify such genes are described. A gene herein designated HpETT1 was isolated from the yeast Hansenula polymorpha. Expression of HpETT1 in an ethanol sensitive mutant H. polymorpha strain designated 7E complimented ethanol sensitivity of the mutant. When multiple copies of the HpETT1 were integrated into the genome and overexpressed, the transformed strain demonstrated approximately 10-fold greater resistance to ethanol and resistance to the protein misfolding agent AZC. Expression of HpETT1 also increased ethanol tolerance in Saccharomyces cerevisiae. HpEtt1 has 39% sequence identity to a previously identified protein from S. cerevisiae denoted MPE1, however, the MPE1 gene does not confer ethanol resistance to the 7E mutant.Type: ApplicationFiled: July 21, 2016Publication date: August 3, 2017Inventors: Charles Abbas, Andriy Sibirny, Andriy Voronovsky, Olena Ishchuk
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Publication number: 20170218403Abstract: A system and method for controlling bacteria, especially lactic and acetic acid bacteria in the production of ethanol using an organic oxidizing compound in combination with an inorganic oxidizer is provided. Particularly, a mixture of one or more peroxy acids and one or more peroxide compounds is introduced into a fermentation mash so as to inhibit or reduce levels of bacteria that compete with yeast for the fermentation sugars. The peroxy acid and peroxide compounds largely are consumed during the fermentation process and are generally not present in the fermentation by-products, especially recovered distiller's grains.Type: ApplicationFiled: April 20, 2017Publication date: August 3, 2017Inventors: Reed Semenza, Michael Welker
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Publication number: 20170218404Abstract: The invention provides schemes for the integration of a fermentation process, with an electrolysis process, and a C1-generating industrial process. In particular, the invention provides process for utilizing electrolysis products, for example H2 and/or O2, to improve the process efficiency of at least one of the fermentation process or the C1-generating industrial process. More particularly, the invention provides a process whereby, H2 generated by electrolysis is used to improve the substrate efficiency for a fermentation process, and the O2 generated by the electrolysis process is used to improve the composition of the C1-containing tail gas generated by the C1-generating industrial process.Type: ApplicationFiled: February 1, 2017Publication date: August 3, 2017Inventors: Sean Dennis Simpson, Robert John Conrado, Christophe Daniel Mihalcea
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Publication number: 20170218405Abstract: Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.Type: ApplicationFiled: April 12, 2017Publication date: August 3, 2017Inventors: Lori Ann Maggio-Hall, Brian James PAUL, Steven Cary ROTHMAN, Rick W. YE
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Publication number: 20170218406Abstract: This document describes biochemical pathways for producing pimeloyl-CoA using a polypeptide having the enzymatic activity of a hydroperoxide lyase to form non-3-enal and 9-oxononanoate from 9-hydroxyperoxyoctadec-10,12-dienoate. Non-3-enal and 9-oxononanoate can be enzymatically converted to pimeloyl-CoA or a salt thereof using one or more polypeptides having the activity of a dehydrogenase, a CoA ligase, an isomerase, a reductase, a thioesterase, a monooxygenase, a hydratase, and/or a thiolase. Pimeloyl-CoA can be enzymatically converted to pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, or 1,7-heptanediol, or corresponding salts thereof. This document also describes recombinant microorganisms producing pimeloyl-CoA, as well as pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, and 1,7-heptanediol, or corresponding salts thereof.Type: ApplicationFiled: January 31, 2017Publication date: August 3, 2017Applicant: INVISTA North America S.a.r.l.Inventors: Alexander Van Eck CONRADIE, Adriana L. BOTES, Alec FOSTER, Changlin CHEN
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Publication number: 20170218407Abstract: The invention described herein presents compositions and methods for a multistep biological and chemical process for the capture and conversion of carbon dioxide and/or other forms of inorganic carbon into organic chemicals including biofuels or other useful industrial, chemical, pharmaceutical, or biomass products. One or more process steps utilizes chemoautotrophic microorganisms to fix inorganic carbon into organic compounds through chemosynthesis. An additional feature described are process steps whereby electron donors used for the chemosynthetic fixation of carbon are generated by chemical or electrochemical means, or are produced from inorganic or waste sources. An additional feature described are process steps for the recovery of useful chemicals produced by the carbon dioxide capture and conversion process, both from chemosynthetic reaction steps, as well as from non-biological reaction steps.Type: ApplicationFiled: April 11, 2017Publication date: August 3, 2017Inventor: John S. Reed
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Publication number: 20170218408Abstract: A method for preparing a fermentation product including lactic acid, the method including: a) treating lignocellulosic material with caustic magnesium salt in the presence of water to provide treated aqueous lignocellulosic material; b) saccharifying the treated aqueous lignocellulosic material in the presence of a hydrolytic enzyme to provide a saccharified aqueous lignocellulosic material comprising fermentable carbohydrate and a solid lignocellulosic fraction; c) simultaneously with step b), fermenting the saccharified aqueous lignocellulosic material in the presence of both a lactic acid forming microorganism and caustic magnesium salt to provide an aqueous fermentation broth comprising magnesium lactate and a solid lignocellulosic fraction; d) recovering magnesium lactate from the broth, wherein the saccharification and the fermentation are carried out simultaneously.Type: ApplicationFiled: July 28, 2015Publication date: August 3, 2017Applicant: PURAC BIOCHEM BVInventors: Peter Johannes Marie BAETS, David SANCHEZ GARCIA, Willem Jacob GROOT, André Banier DE HAAN
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Publication number: 20170218409Abstract: A method of producing acrylate in vivo in a genetically modified bacteria cell is provided including providing a cell with a first one or more nucleic acids encoding a pathway resulting in the production of 3-hydroxypropionate, providing the cell with a second one or more nucleic acids encoding a truncated pcs enzyme lacking a domain converting acrylyl-CoA to propionyl-CoA, providing the cell with a third one or more nucleic acids encoding acrylyl-CoA hydrolase, wherein the cell expresses the first, second and third one or more nucleic acids and produces acrylate.Type: ApplicationFiled: February 1, 2017Publication date: August 3, 2017Inventors: Jameson K. Rogers, George M. Church
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Publication number: 20170218410Abstract: Described here is a method for increasing the transfer of a gas substrate in microbial fermentation, comprising incubating an emulsion comprising an oil phase and an aqueous phase droplet dispersed in the oil phase, and supplying the gas substrate to the oil phase, wherein the aqueous phase droplet comprises a microorganism, and wherein the emulsion is stabilized by a surfactant or an amphiphilic particle that is adsorbed to an interface of the oil phase and the aqueous phase. Also described is an emulsion for microbial fermentation, comprises an oil phase and an aqueous phase droplet dispersed in the oil phase, wherein the aqueous phase droplet comprises a microorganism, wherein the emulsion comprises a gas substrate externally-supplied to the oil phase, and wherein the emulsion is stabilized by a surfactant or an amphiphilic particle that is adsorbed to an interface of the oil phase and the aqueous phase.Type: ApplicationFiled: January 27, 2017Publication date: August 3, 2017Applicant: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Sindy Tang, Craig Criddle, Jaewook Myung, Minkyu Kim
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Publication number: 20170218411Abstract: The purpose of the present invention is to provide a method for enhancing the production quantity of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HH)) having a high fraction of 3-hydroxyhexanoate (3HH) using a saccharide or glycerol as a starting material. The present invention provides: a method for producing a P(3HB-co-3HH) copolymer including performing transformation by homologous recombination of a crotonyl-CoA reductase gene in a chromosome of a recombinant strain of Cupriavidus necator endowed with the ability to produce P(3HB-co-3HH), or performing transformation by introducing an autonomous replication vector in which the crotonyl-CoA reductase gene is incorporated in the aforementioned strain, and cultivating the transformant in a medium containing a saccharide or glycerol as a carbon source; and a method for enhancing the production quantity of the copolymer and/or the fraction of 3HHx in the copolymer.Type: ApplicationFiled: August 4, 2015Publication date: August 3, 2017Applicants: Tokyo Institute Technology, Kaneka CorporationInventors: Toshiaki Fukui, Izumi Orita
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Publication number: 20170218412Abstract: The invention relates to a method for triggering triacylglycerols (TAG) accumulation in microalgae comprising the step of contacting a source of exogenous nitroxide (NO) with said microalgae in their growth medium.Type: ApplicationFiled: January 27, 2017Publication date: August 3, 2017Inventors: Eric Marechal, Lina Juana Dolch
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Publication number: 20170218413Abstract: The present invention relates to methods for degrading or converting a cellulose-containing material, comprising: treating the cellulose-containing material with an effective amount of a cellulolytic enzyme composition comprising a polypeptide having cellulolytic enhancing activity, and one or more (several) components selected from the group consisting of a CEL7 polypeptide having endoglucanase activity, a CEL12 polypeptide having endoglucanase activity, a CEL45 polypeptide having endoglucanase activity, a CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain, and a CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain. The present invention also relates to such cellulolytic enzyme compositions.Type: ApplicationFiled: April 10, 2017Publication date: August 3, 2017Inventor: Keith McFarland
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Publication number: 20170218414Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: ApplicationFiled: September 12, 2016Publication date: August 3, 2017Inventors: Mark J. BURK, Anthony P. BURGARD, Robin E. OSTERHOUT, Priti PHARKYA
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Publication number: 20170218415Abstract: The present invention provides an alga that is modified to have suppressed expression of ATG8 through (i) overexpression of MEX1 and/or (ii) silencing of ATG8 with a miRNA and exhibits increased photosynthetic productivity to achieve increased biomass productivity in algal cells. The invention further provides a method of producing such a modified alga, a method of biomass production using such a modified alga, and starch produced using such a modified alga.Type: ApplicationFiled: May 26, 2015Publication date: August 3, 2017Inventors: Ken'ichi OGAWA, Masanobu NISHIKAWA, Kazuya KIYOKAWA
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Publication number: 20170218416Abstract: The present invention provides for compositions and methods for single-molecule construction of DNA. The present invention provides for a method comprising: (a) providing a reaction chamber comprising a solid support bound to a single starter double-stranded (ds) DNA molecule comprising a free end, (b) introducing one or more extension molecules and one or more enzymes capable of joining a payload region of an extension molecule to the free end of starter dsDNA molecule to the reaction chamber wherein the extension molecule comprises an cleavable linker.Type: ApplicationFiled: May 18, 2015Publication date: August 3, 2017Applicant: The Regents of the University of CaliforniaInventors: Daniel H. Arlow, William J. Holtz, Lane J. Weaver, Jay D. Keasling
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Publication number: 20170218417Abstract: A process for the enzymatic regeneration of the redox cofactors NAD+/NADH and NADP+/NADPH in a one-pot reaction, wherein, as a result of at least two further enzymatically catalyzed redox reactions proceeding in the same reaction batch (product-forming reactions), one of the two redox cofactors accumulates in its reduced form and, respectively, the other one in its oxidized form, characterized in that a) in the regeneration reaction which reconverts the reduced cofactor into its original oxidized form, oxygen or a compound of general formula R1C(O)COOH is reduced, and b) in the regeneration reaction which reconverts the oxidized cofactor into its original reduced form, a compound of general formula R2CH(OH)R3 is oxidized and wherein R1, R2 and R3 in the compounds have different meanings.Type: ApplicationFiled: April 18, 2017Publication date: August 3, 2017Inventors: Ortwin Ertl, Nicole Staunig, Marta Sut, Bernd Mayer
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Publication number: 20170218418Abstract: The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.Type: ApplicationFiled: August 7, 2015Publication date: August 3, 2017Inventors: Veronique Douchin, Michael Dalgaard Mikkelsen, Iben Møller-Hansen
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Publication number: 20170218419Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.Type: ApplicationFiled: December 16, 2016Publication date: August 3, 2017Inventors: Ganesh M. KISHORE, Michael MOTION, Paula M. HICKS, Jorgen HANSEN, Jens HOUGHTON-LARSEN, Esben Halkjaer HANSEN, Michael Dalgaard MIKKELSEN, Sabina TAVARES, Charlotte BLOM
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Publication number: 20170218420Abstract: Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).Type: ApplicationFiled: April 12, 2017Publication date: August 3, 2017Applicant: Conagen Inc.Inventors: Guohong Mao, Xiaodan Yu
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Publication number: 20170218421Abstract: Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).Type: ApplicationFiled: April 12, 2017Publication date: August 3, 2017Applicant: Conagen Inc.Inventors: Guohong Mao, Xiaodan Yu
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Publication number: 20170218422Abstract: Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification.Type: ApplicationFiled: February 9, 2017Publication date: August 3, 2017Inventors: Ellen M. BRUNE, Robert R. Beitle, JR., Mohammad M. Ataai, Patrick R. Bartlow, Ralph L. Henry
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Publication number: 20170218423Abstract: An electrochemical measuring method uses an electrochemical measuring device in which a measuring liquid is filled into a well. The electrochemical measuring method includes: a step of applying a measuring voltage to a working electrode and measuring a value of a first current flowing in the working electrode; a step of applying a non-measuring voltage to the working electrode; a step of introducing the biological sample into a container; and a step of applying the measuring voltage to the working electrode and measuring a value of a second current flowing in the working electrode.Type: ApplicationFiled: September 17, 2015Publication date: August 3, 2017Inventors: KAORU HIRAMOTO, MASAHIRO YASUMI, HIROSHI USHIO, ATSUSHI SHUNORI
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Publication number: 20170218424Abstract: Systems, methods, and devices are described herein for identifying, monitoring, isolating, or selecting a cell having a predefined characteristic in a mixed population of cells utilizing a combination of any one or more of iDEP, a region of localized field enhancement, a variable frequency electric field, a wide bandwidth amplifier, and/or an imaging apparatus.Type: ApplicationFiled: October 9, 2015Publication date: August 3, 2017Inventors: Nathan Swami, Yi-Hsuan Su, Cirle Alcantara Warren, Ali Rohani, Vahid Farmehini
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Publication number: 20170218425Abstract: The present disclosure relates to a cartridge, detection module, system, and kit for cell and particle detection and analysis. Devices disclosed herein may include at least an optical source, a fluidic chip, and a detection module, wherein the sample flows within the fluidic chip past a detection window, where the cells or particles are imaged by an image acquisition and analysis module that may include an optical detector. The image acquisition and analysis module may count the cells or particles of interest in real-time, or near real-time, or the module may capture images of the cells in order to analyze the sample from combined images at a later time.Type: ApplicationFiled: September 29, 2015Publication date: August 3, 2017Inventors: Lu CHEN, James Jiahua DOU, James Andrew FRASER, Rakesh Kumar NAYYAR