Patents Issued in August 3, 2017
  • Publication number: 20170218376
    Abstract: In certain aspects, provided herein are RNA complexes (e.g., asymmetric RNA complexes, such as asiRNAs or cell penetrating asiRNAs) that inhibit IL4R?, TRPA1, and/or F2RL1 expression and are therefore useful for treating atopic dermatitis or asthma.
    Type: Application
    Filed: February 1, 2017
    Publication date: August 3, 2017
    Inventors: Dong-Ki Lee, Sun Woo Hong, Hanna Lee, Dayeon Yu, Ji Eom
  • Publication number: 20170218377
    Abstract: The present invention relates to recombinant Trichoderma host cells producing Aspergillus fumigatus cellulolytic enzyme compositions and methods of producing and using the compositions.
    Type: Application
    Filed: April 17, 2017
    Publication date: August 3, 2017
    Applicant: Novozymes, Inc.
    Inventors: Jeffrey Shasky, Brett McBrayer
  • Publication number: 20170218378
    Abstract: The present invention relates to compositions and methods for delivering lysosomal proteins. The compositions and methods described herein permit the targeted delivery of exogenous lysosomal proteins to cell surface proteins that allow their internalization via non-clathrin pathways. The present invention further relates to the use of the compositions and methods for enzyme replacement therapy of lysosomal storage diseases. Nucleic acids, recombinant cells and kits useful for making and using the compositions of the invention are also provided.
    Type: Application
    Filed: April 18, 2017
    Publication date: August 3, 2017
    Inventors: Silvia MURO GALINDO, Vladimir R. MUZYKANTOV, Edward Howard SCHUCHMAN
  • Publication number: 20170218379
    Abstract: A self-regulating gene expression construct comprises a single promoter in operative association with a repressor sequence (e.g., bacterial repressor lacI or gaiR), operator sequence(s) responsive to the expressed repressor protein, and a transgene. A dual-regulating construct comprises a single promoter controlling expression of a bacterial repressor sequence and a transgene, and which, in the presence of a first inducer molecule, transcribes the transgene and repressor; and a ribozyme in association with an aptamer sequence, the aptamer sequence capable of interacting with a second inducer molecule to terminate mRNA degradation by the ribozyme. Also provided are recombinant vectors or viruses containing the self-regulating or dual self-regulating constructs and cells containing the vectors. Such compositions are useful in methods of treating a diseases using gene therapy.
    Type: Application
    Filed: July 31, 2015
    Publication date: August 3, 2017
    Inventors: Mitchell LEWIS, Jean BENNETT, Luk VANDENBERGHE, Matthew SOCHOR, Theodore G. DRIVAS
  • Publication number: 20170218380
    Abstract: Described herein are techniques for directional cloning an insert DNA segments into a target vector. The techniques mix the target vector, the insert DNA segment, a restriction enzyme, and a DNA ligase to generate a recombinant DNA molecule.
    Type: Application
    Filed: April 7, 2017
    Publication date: August 3, 2017
    Inventors: Lei Xiao, Zhao Wu, Mao Bi
  • Publication number: 20170218381
    Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
    Type: Application
    Filed: April 7, 2017
    Publication date: August 3, 2017
    Applicant: Novozymes A/S
    Inventors: Anne Breuner, Michael Dolberg Rasmussen
  • Publication number: 20170218382
    Abstract: An expression vector is disclosed which contains a promoter DNA; a DNA encoding a peptide having a defined amino acid sequence and having secretion signal activity; and a DNA encoding an intended protein or a cloning site for insertion of the DNA encoding an intended protein. An expression vector is also disclosed which contains a promoter DNA; a DNA encoding any peptide having a defined amino acid sequence and having secretion signal activity; a DNA encoding an intended protein or a cloning site for insertion of the DNA encoding an intended protein; and a DNA encoding an anchor domain. The peptide having secretion signal activity allows for secretory production and cell surface display of a protein with high activity, in yeast.
    Type: Application
    Filed: July 30, 2015
    Publication date: August 3, 2017
    Applicant: National University Corporation Kobe University
    Inventors: Akihiko KONDO, Tomohisa HASUNUMA, Kentaro INOKUMA
  • Publication number: 20170218383
    Abstract: Transcription factor polynucleotides and polypeptides incorporated into nucleic acid constructs, including expression vectors, have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these constructs have been shown to have increased tolerance to an abiotic stress (in some cases, to more than one abiotic stress), increased growth, and/or increased biomass. The abiotic stress may include, for example, salt, hyperosmotic stress, water deficit, heat, cold, drought, and/or low nutrient conditions.
    Type: Application
    Filed: April 14, 2017
    Publication date: August 3, 2017
    Inventors: T. Lynne Reuber, Oliver J. Ratcliffe, Frederick D. Hempel, Luc J. Adam, Cai-Zhong Jiang, Robert A. Creelman, Jose Luis Riechmann, Jacqueline E. Heard, Raymond R. Samaha, Pierre E. Broun, Magnus Hertzberg, Torgny Nasholm
  • Publication number: 20170218384
    Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for a Setaria italica (Foxtail Millet) (SI-Ubiquitin) and Sorghum bicolor (SB-Ubiquitin) regulatory regions. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
    Type: Application
    Filed: August 4, 2015
    Publication date: August 3, 2017
    Inventors: SHANE ABBITT, MICHELLE VAN ALLEN
  • Publication number: 20170218385
    Abstract: Various embodiments are directed to transgenic plants, including transgenic tobacco plants and derivative seeds, genetically modified to impede the transport of Cadmium (Cd) from the root system to aerial portions of transgenic plants by reducing the expression levels of HMA-related transporters. Various embodiments are directed to transgenic tobacco plants genetically modified to stably express a RNAi construct encoding RNAi polynucleotides that enable the degradation of endogenous NtHMA RNA variants. Reduced expression of NtHMA transporters in transgenic plants results in substantially reduced content of Cadmium (Cd) in the leaf lamina. Various consumable products that are substantially free or substantially reduced in Cd content can be produced by incorporating leaves derived from transgenic tobacco plants modified to reduce the expression of NtHMA transporters.
    Type: Application
    Filed: April 12, 2017
    Publication date: August 3, 2017
    Applicant: Philip Morris USA Inc.
    Inventors: Alec J Hayes, Chengalrayan Kudithipudi, Rutger S van der Hoeven
  • Publication number: 20170218386
    Abstract: The invention relates to genetically modified agricultural plants with increased oil content in vegetative tissues, as well as to expression systems, plant cells, seeds and vegetative tissues related thereto.
    Type: Application
    Filed: April 13, 2017
    Publication date: August 3, 2017
    Inventors: Christoph Benning, Sanjaya
  • Publication number: 20170218387
    Abstract: Compositions and methods for improving plant growth are provided herein. Compositions comprise promoter sequences that direct expression of an operably linked nucleotide in a developmentally regulated manner. Polynucleotides, polypeptides, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
    Type: Application
    Filed: July 23, 2016
    Publication date: August 3, 2017
    Applicant: Benson Hill Biosystems, Inc.
    Inventors: Thomas P. Brutnell, Douglas W. Bryant, Todd Christopher Mockler, Lin Wang
  • Publication number: 20170218388
    Abstract: A method of improving abiotic stress tolerance of a plant is provided. The method comprising genetically modifying the plant to express miRNA167 in an abiotic stress responsive manner, wherein a level of expression of total miR167 under the abiotic stress conditions is selected not exceeding 10 fold compared to same in the plant when grown under optimal conditions, thereby improving abiotic stress tolerance of the plant.
    Type: Application
    Filed: April 13, 2017
    Publication date: August 3, 2017
    Applicant: A.B. Seeds Ltd.
    Inventors: Orly NOIVIRT-BRIK, Rudy MAOR, Amir AVNIEL
  • Publication number: 20170218389
    Abstract: The present disclosure provides methods for regulating stomata in plants, improving drought tolerance, and increasing resistance to bacterial pathogens through overexpression of genes NHR1 or GCN4. Also provided are transgenic plants with improved drought tolerance and increased resistance to bacterial pathogens produced by such methods.
    Type: Application
    Filed: January 11, 2017
    Publication date: August 3, 2017
    Inventors: Kirankumar Mysore, Amita Kaundal, Seonghee Lee
  • Publication number: 20170218390
    Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.
    Type: Application
    Filed: January 31, 2017
    Publication date: August 3, 2017
    Inventors: Kenneth E. Narva, Sarah Worden, Meghan Frey, Murugesan Rangasamy, Premchand Gandra, Wendy Lo, Elane Fishilevich, Rainer Fischer, Andreas Vilcinskas, Eileen Knorr
  • Publication number: 20170218391
    Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.
    Type: Application
    Filed: January 31, 2017
    Publication date: August 3, 2017
    Inventors: Kenneth E. Narva, Sarah Worden, Meghan Frey, Murugesan Rangasamy, Premchand Gandra, Wendy Lo, Elane Fishilevich, Rainer Fischer, Andreas Vilcinskas, Eileen Knorr
  • Publication number: 20170218392
    Abstract: The present invention relates to a new gene fragment derived from Chinese hamster ovary (CHO) cell for enhancement of recombinant protein expression in animal cells and a use thereof. It has been found that using the vector comprising a gene fragment of the present invention enhances the expression of a target protein in animal cells. Accordingly, the vector comprising a gene fragment of the present invention could be usefully used in the production of biopharmaceuticals such as therapeutic antibodies, etc.
    Type: Application
    Filed: June 16, 2015
    Publication date: August 3, 2017
    Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
    Inventors: Eun Gyo LEE, Hong-Weon LEE, Shin Young KANG, Yeon-Gu KIM, Joon Ki JUNG, Jungoh AHN, Seung Hee KANG, Chun Sug KIM, Hyeok Won LEE, Jin Gyeom LEE
  • Publication number: 20170218393
    Abstract: The present invention discloses methods and compositions for modulating the quality of an immune response to a target antigen in a mammal, which response results from the expression of a polynucleotide that encodes at least a portion of the target antigen, wherein the quality is modulated by replacing at least one codon of the polynucleotide with a synonymous codon that has a higher or lower preference of usage by the mammal to confer the immune response than the codon it replaces.
    Type: Application
    Filed: January 17, 2017
    Publication date: August 3, 2017
    Inventors: Ian Hector Frazer, Julia Louise Dutton
  • Publication number: 20170218394
    Abstract: Methods of producing hepatocyte lineage cells are provided. The method can include transfecting a cell with one or more expression vectors. For example, a cell can be transfected with a first expression vector containing a first gene that encodes CCAAT/enhancer binding protein alpha (CEBPA), a second expression vector containing a second gene that encodes CCAAT/enhancer binding protein beta (CEBPB), a third expression vector containing a third gene that encodes forkhead box A1 (FOXA1), and a fourth expression vector containing a fourth gene that encodes forkhead box A3 (FOXA3). The method can include culturing the transfected cell obtained in a growth environment. The transfected cell can be cultured in Williams' E medium, ReproFF (feeder-free media maintaining pluripotency) medium, or both. Transfected and/or hepatocyte lineage cells obtained by a method of the present invention are also provided.
    Type: Application
    Filed: January 27, 2017
    Publication date: August 3, 2017
    Applicant: National University Corporation Chiba University
    Inventor: Minoru Tomizawa
  • Publication number: 20170218395
    Abstract: Provided herein are methods related to co-packaging of multiple rAAV particles, e.g., by introducing multiple nucleic acid vectors encoding proteins or polypeptides or RNAs of interest into a single cell preparation.
    Type: Application
    Filed: June 20, 2015
    Publication date: August 3, 2017
    Applicant: University of Florida Research Foundation, Inc.
    Inventors: Barry John Byrne, Phillip A. Doerfer, Nathalie Clement
  • Publication number: 20170218396
    Abstract: A cell modified for obtaining increased proliferative capacity, decreased aging and enhanced regenerative capacity, its modification involving HOXB7 overexpression obtained using a gene vector that can insert the coding sequence into the cell, thereby affording increased protein production.
    Type: Application
    Filed: July 30, 2015
    Publication date: August 3, 2017
    Inventors: Massimo Dominici, Olivia Candini
  • Publication number: 20170218397
    Abstract: A method, system, and apparatus for treating a patient with HIV. A vector can be modified from a thymidine kinase gene. The modified vector is expressed in the presence of tat RNA. The modified vector is then package and delivered to HIV-infected cells. The replication of HIV is inhibited by eliminating infected cells in the presence of Ganciclovir. Modified cells are then selected utilizing transient tat RNA transfection and GFP expression. Vector-modified stem cells are then selected for transplantation back into the patient, thereby producing a normal immune system in the patient when the modified vector remains dormant in the absence of HIV tat.
    Type: Application
    Filed: July 20, 2015
    Publication date: August 3, 2017
    Inventors: Himanshu Garg, Anjali Joshi
  • Publication number: 20170218398
    Abstract: Methods and compositions for selectively targeting cancerous cells for genetic manipulation based on cancer specific sequence motifs (CSSMs), which are formed as a result of chromosomal rearrangement, and cells produced by said methods.
    Type: Application
    Filed: January 30, 2017
    Publication date: August 3, 2017
    Inventors: Markus Alexander Brown, Reinhard Ebner
  • Publication number: 20170218399
    Abstract: Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.
    Type: Application
    Filed: April 12, 2017
    Publication date: August 3, 2017
    Inventors: Wojtek Auerbach, Thomas DeChiara, William Poueymirou, David Frendewey, David Valenzuela
  • Publication number: 20170218400
    Abstract: The present invention relates in part to nucleic acids encoding proteins, therapeutics comprising nucleic acids encoding proteins, methods for inducing cells to express proteins using nucleic acids, methods, kits and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods and products for altering the DNA sequence of a cell are described, as are methods and products for inducing cells to express proteins using synthetic RNA molecules. Therapeutics comprising nucleic acids encoding gene-editing proteins are also described.
    Type: Application
    Filed: April 13, 2017
    Publication date: August 3, 2017
    Inventors: Matthew Angel, Christopher Rohde
  • Publication number: 20170218401
    Abstract: Provided are genetically engineered microorganism that catalyze the synthesis of propane and/or butanol from a suitable substrate such as glucose. Also provided are methods of engineering said genetically engineered microorganism and methods of producing propane and/or butanol using the genetically engineered microorganism.
    Type: Application
    Filed: February 1, 2017
    Publication date: August 3, 2017
    Inventors: Nigel Scrutton, Patrik Jones, Navya Menon
  • Publication number: 20170218402
    Abstract: Methods of identifying genes conferring ethanol tolerance in yeasts, genes that confer ethanol tolerance, and mutant strains used to identify such genes are described. A gene herein designated HpETT1 was isolated from the yeast Hansenula polymorpha. Expression of HpETT1 in an ethanol sensitive mutant H. polymorpha strain designated 7E complimented ethanol sensitivity of the mutant. When multiple copies of the HpETT1 were integrated into the genome and overexpressed, the transformed strain demonstrated approximately 10-fold greater resistance to ethanol and resistance to the protein misfolding agent AZC. Expression of HpETT1 also increased ethanol tolerance in Saccharomyces cerevisiae. HpEtt1 has 39% sequence identity to a previously identified protein from S. cerevisiae denoted MPE1, however, the MPE1 gene does not confer ethanol resistance to the 7E mutant.
    Type: Application
    Filed: July 21, 2016
    Publication date: August 3, 2017
    Inventors: Charles Abbas, Andriy Sibirny, Andriy Voronovsky, Olena Ishchuk
  • Publication number: 20170218403
    Abstract: A system and method for controlling bacteria, especially lactic and acetic acid bacteria in the production of ethanol using an organic oxidizing compound in combination with an inorganic oxidizer is provided. Particularly, a mixture of one or more peroxy acids and one or more peroxide compounds is introduced into a fermentation mash so as to inhibit or reduce levels of bacteria that compete with yeast for the fermentation sugars. The peroxy acid and peroxide compounds largely are consumed during the fermentation process and are generally not present in the fermentation by-products, especially recovered distiller's grains.
    Type: Application
    Filed: April 20, 2017
    Publication date: August 3, 2017
    Inventors: Reed Semenza, Michael Welker
  • Publication number: 20170218404
    Abstract: The invention provides schemes for the integration of a fermentation process, with an electrolysis process, and a C1-generating industrial process. In particular, the invention provides process for utilizing electrolysis products, for example H2 and/or O2, to improve the process efficiency of at least one of the fermentation process or the C1-generating industrial process. More particularly, the invention provides a process whereby, H2 generated by electrolysis is used to improve the substrate efficiency for a fermentation process, and the O2 generated by the electrolysis process is used to improve the composition of the C1-containing tail gas generated by the C1-generating industrial process.
    Type: Application
    Filed: February 1, 2017
    Publication date: August 3, 2017
    Inventors: Sean Dennis Simpson, Robert John Conrado, Christophe Daniel Mihalcea
  • Publication number: 20170218405
    Abstract: Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
    Type: Application
    Filed: April 12, 2017
    Publication date: August 3, 2017
    Inventors: Lori Ann Maggio-Hall, Brian James PAUL, Steven Cary ROTHMAN, Rick W. YE
  • Publication number: 20170218406
    Abstract: This document describes biochemical pathways for producing pimeloyl-CoA using a polypeptide having the enzymatic activity of a hydroperoxide lyase to form non-3-enal and 9-oxononanoate from 9-hydroxyperoxyoctadec-10,12-dienoate. Non-3-enal and 9-oxononanoate can be enzymatically converted to pimeloyl-CoA or a salt thereof using one or more polypeptides having the activity of a dehydrogenase, a CoA ligase, an isomerase, a reductase, a thioesterase, a monooxygenase, a hydratase, and/or a thiolase. Pimeloyl-CoA can be enzymatically converted to pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, or 1,7-heptanediol, or corresponding salts thereof. This document also describes recombinant microorganisms producing pimeloyl-CoA, as well as pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, and 1,7-heptanediol, or corresponding salts thereof.
    Type: Application
    Filed: January 31, 2017
    Publication date: August 3, 2017
    Applicant: INVISTA North America S.a.r.l.
    Inventors: Alexander Van Eck CONRADIE, Adriana L. BOTES, Alec FOSTER, Changlin CHEN
  • Publication number: 20170218407
    Abstract: The invention described herein presents compositions and methods for a multistep biological and chemical process for the capture and conversion of carbon dioxide and/or other forms of inorganic carbon into organic chemicals including biofuels or other useful industrial, chemical, pharmaceutical, or biomass products. One or more process steps utilizes chemoautotrophic microorganisms to fix inorganic carbon into organic compounds through chemosynthesis. An additional feature described are process steps whereby electron donors used for the chemosynthetic fixation of carbon are generated by chemical or electrochemical means, or are produced from inorganic or waste sources. An additional feature described are process steps for the recovery of useful chemicals produced by the carbon dioxide capture and conversion process, both from chemosynthetic reaction steps, as well as from non-biological reaction steps.
    Type: Application
    Filed: April 11, 2017
    Publication date: August 3, 2017
    Inventor: John S. Reed
  • Publication number: 20170218408
    Abstract: A method for preparing a fermentation product including lactic acid, the method including: a) treating lignocellulosic material with caustic magnesium salt in the presence of water to provide treated aqueous lignocellulosic material; b) saccharifying the treated aqueous lignocellulosic material in the presence of a hydrolytic enzyme to provide a saccharified aqueous lignocellulosic material comprising fermentable carbohydrate and a solid lignocellulosic fraction; c) simultaneously with step b), fermenting the saccharified aqueous lignocellulosic material in the presence of both a lactic acid forming microorganism and caustic magnesium salt to provide an aqueous fermentation broth comprising magnesium lactate and a solid lignocellulosic fraction; d) recovering magnesium lactate from the broth, wherein the saccharification and the fermentation are carried out simultaneously.
    Type: Application
    Filed: July 28, 2015
    Publication date: August 3, 2017
    Applicant: PURAC BIOCHEM BV
    Inventors: Peter Johannes Marie BAETS, David SANCHEZ GARCIA, Willem Jacob GROOT, André Banier DE HAAN
  • Publication number: 20170218409
    Abstract: A method of producing acrylate in vivo in a genetically modified bacteria cell is provided including providing a cell with a first one or more nucleic acids encoding a pathway resulting in the production of 3-hydroxypropionate, providing the cell with a second one or more nucleic acids encoding a truncated pcs enzyme lacking a domain converting acrylyl-CoA to propionyl-CoA, providing the cell with a third one or more nucleic acids encoding acrylyl-CoA hydrolase, wherein the cell expresses the first, second and third one or more nucleic acids and produces acrylate.
    Type: Application
    Filed: February 1, 2017
    Publication date: August 3, 2017
    Inventors: Jameson K. Rogers, George M. Church
  • Publication number: 20170218410
    Abstract: Described here is a method for increasing the transfer of a gas substrate in microbial fermentation, comprising incubating an emulsion comprising an oil phase and an aqueous phase droplet dispersed in the oil phase, and supplying the gas substrate to the oil phase, wherein the aqueous phase droplet comprises a microorganism, and wherein the emulsion is stabilized by a surfactant or an amphiphilic particle that is adsorbed to an interface of the oil phase and the aqueous phase. Also described is an emulsion for microbial fermentation, comprises an oil phase and an aqueous phase droplet dispersed in the oil phase, wherein the aqueous phase droplet comprises a microorganism, wherein the emulsion comprises a gas substrate externally-supplied to the oil phase, and wherein the emulsion is stabilized by a surfactant or an amphiphilic particle that is adsorbed to an interface of the oil phase and the aqueous phase.
    Type: Application
    Filed: January 27, 2017
    Publication date: August 3, 2017
    Applicant: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Sindy Tang, Craig Criddle, Jaewook Myung, Minkyu Kim
  • Publication number: 20170218411
    Abstract: The purpose of the present invention is to provide a method for enhancing the production quantity of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HH)) having a high fraction of 3-hydroxyhexanoate (3HH) using a saccharide or glycerol as a starting material. The present invention provides: a method for producing a P(3HB-co-3HH) copolymer including performing transformation by homologous recombination of a crotonyl-CoA reductase gene in a chromosome of a recombinant strain of Cupriavidus necator endowed with the ability to produce P(3HB-co-3HH), or performing transformation by introducing an autonomous replication vector in which the crotonyl-CoA reductase gene is incorporated in the aforementioned strain, and cultivating the transformant in a medium containing a saccharide or glycerol as a carbon source; and a method for enhancing the production quantity of the copolymer and/or the fraction of 3HHx in the copolymer.
    Type: Application
    Filed: August 4, 2015
    Publication date: August 3, 2017
    Applicants: Tokyo Institute Technology, Kaneka Corporation
    Inventors: Toshiaki Fukui, Izumi Orita
  • Publication number: 20170218412
    Abstract: The invention relates to a method for triggering triacylglycerols (TAG) accumulation in microalgae comprising the step of contacting a source of exogenous nitroxide (NO) with said microalgae in their growth medium.
    Type: Application
    Filed: January 27, 2017
    Publication date: August 3, 2017
    Inventors: Eric Marechal, Lina Juana Dolch
  • Publication number: 20170218413
    Abstract: The present invention relates to methods for degrading or converting a cellulose-containing material, comprising: treating the cellulose-containing material with an effective amount of a cellulolytic enzyme composition comprising a polypeptide having cellulolytic enhancing activity, and one or more (several) components selected from the group consisting of a CEL7 polypeptide having endoglucanase activity, a CEL12 polypeptide having endoglucanase activity, a CEL45 polypeptide having endoglucanase activity, a CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain, and a CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain. The present invention also relates to such cellulolytic enzyme compositions.
    Type: Application
    Filed: April 10, 2017
    Publication date: August 3, 2017
    Inventor: Keith McFarland
  • Publication number: 20170218414
    Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.
    Type: Application
    Filed: September 12, 2016
    Publication date: August 3, 2017
    Inventors: Mark J. BURK, Anthony P. BURGARD, Robin E. OSTERHOUT, Priti PHARKYA
  • Publication number: 20170218415
    Abstract: The present invention provides an alga that is modified to have suppressed expression of ATG8 through (i) overexpression of MEX1 and/or (ii) silencing of ATG8 with a miRNA and exhibits increased photosynthetic productivity to achieve increased biomass productivity in algal cells. The invention further provides a method of producing such a modified alga, a method of biomass production using such a modified alga, and starch produced using such a modified alga.
    Type: Application
    Filed: May 26, 2015
    Publication date: August 3, 2017
    Inventors: Ken'ichi OGAWA, Masanobu NISHIKAWA, Kazuya KIYOKAWA
  • Publication number: 20170218416
    Abstract: The present invention provides for compositions and methods for single-molecule construction of DNA. The present invention provides for a method comprising: (a) providing a reaction chamber comprising a solid support bound to a single starter double-stranded (ds) DNA molecule comprising a free end, (b) introducing one or more extension molecules and one or more enzymes capable of joining a payload region of an extension molecule to the free end of starter dsDNA molecule to the reaction chamber wherein the extension molecule comprises an cleavable linker.
    Type: Application
    Filed: May 18, 2015
    Publication date: August 3, 2017
    Applicant: The Regents of the University of California
    Inventors: Daniel H. Arlow, William J. Holtz, Lane J. Weaver, Jay D. Keasling
  • Publication number: 20170218417
    Abstract: A process for the enzymatic regeneration of the redox cofactors NAD+/NADH and NADP+/NADPH in a one-pot reaction, wherein, as a result of at least two further enzymatically catalyzed redox reactions proceeding in the same reaction batch (product-forming reactions), one of the two redox cofactors accumulates in its reduced form and, respectively, the other one in its oxidized form, characterized in that a) in the regeneration reaction which reconverts the reduced cofactor into its original oxidized form, oxygen or a compound of general formula R1C(O)COOH is reduced, and b) in the regeneration reaction which reconverts the oxidized cofactor into its original reduced form, a compound of general formula R2CH(OH)R3 is oxidized and wherein R1, R2 and R3 in the compounds have different meanings.
    Type: Application
    Filed: April 18, 2017
    Publication date: August 3, 2017
    Inventors: Ortwin Ertl, Nicole Staunig, Marta Sut, Bernd Mayer
  • Publication number: 20170218418
    Abstract: The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.
    Type: Application
    Filed: August 7, 2015
    Publication date: August 3, 2017
    Inventors: Veronique Douchin, Michael Dalgaard Mikkelsen, Iben Møller-Hansen
  • Publication number: 20170218419
    Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.
    Type: Application
    Filed: December 16, 2016
    Publication date: August 3, 2017
    Inventors: Ganesh M. KISHORE, Michael MOTION, Paula M. HICKS, Jorgen HANSEN, Jens HOUGHTON-LARSEN, Esben Halkjaer HANSEN, Michael Dalgaard MIKKELSEN, Sabina TAVARES, Charlotte BLOM
  • Publication number: 20170218420
    Abstract: Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
    Type: Application
    Filed: April 12, 2017
    Publication date: August 3, 2017
    Applicant: Conagen Inc.
    Inventors: Guohong Mao, Xiaodan Yu
  • Publication number: 20170218421
    Abstract: Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
    Type: Application
    Filed: April 12, 2017
    Publication date: August 3, 2017
    Applicant: Conagen Inc.
    Inventors: Guohong Mao, Xiaodan Yu
  • Publication number: 20170218422
    Abstract: Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification.
    Type: Application
    Filed: February 9, 2017
    Publication date: August 3, 2017
    Inventors: Ellen M. BRUNE, Robert R. Beitle, JR., Mohammad M. Ataai, Patrick R. Bartlow, Ralph L. Henry
  • Publication number: 20170218423
    Abstract: An electrochemical measuring method uses an electrochemical measuring device in which a measuring liquid is filled into a well. The electrochemical measuring method includes: a step of applying a measuring voltage to a working electrode and measuring a value of a first current flowing in the working electrode; a step of applying a non-measuring voltage to the working electrode; a step of introducing the biological sample into a container; and a step of applying the measuring voltage to the working electrode and measuring a value of a second current flowing in the working electrode.
    Type: Application
    Filed: September 17, 2015
    Publication date: August 3, 2017
    Inventors: KAORU HIRAMOTO, MASAHIRO YASUMI, HIROSHI USHIO, ATSUSHI SHUNORI
  • Publication number: 20170218424
    Abstract: Systems, methods, and devices are described herein for identifying, monitoring, isolating, or selecting a cell having a predefined characteristic in a mixed population of cells utilizing a combination of any one or more of iDEP, a region of localized field enhancement, a variable frequency electric field, a wide bandwidth amplifier, and/or an imaging apparatus.
    Type: Application
    Filed: October 9, 2015
    Publication date: August 3, 2017
    Inventors: Nathan Swami, Yi-Hsuan Su, Cirle Alcantara Warren, Ali Rohani, Vahid Farmehini
  • Publication number: 20170218425
    Abstract: The present disclosure relates to a cartridge, detection module, system, and kit for cell and particle detection and analysis. Devices disclosed herein may include at least an optical source, a fluidic chip, and a detection module, wherein the sample flows within the fluidic chip past a detection window, where the cells or particles are imaged by an image acquisition and analysis module that may include an optical detector. The image acquisition and analysis module may count the cells or particles of interest in real-time, or near real-time, or the module may capture images of the cells in order to analyze the sample from combined images at a later time.
    Type: Application
    Filed: September 29, 2015
    Publication date: August 3, 2017
    Inventors: Lu CHEN, James Jiahua DOU, James Andrew FRASER, Rakesh Kumar NAYYAR