Patents Issued in August 3, 2017
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Publication number: 20170218426Abstract: This disclosure is related to systems and methods for rapid determination of microorganism growth and antimicrobial agent susceptibility and/or resistance.Type: ApplicationFiled: April 11, 2017Publication date: August 3, 2017Applicant: Accelerate Diagnostics, Inc.Inventors: Alena Shamsheyeva, David C. Howson, Steven W. Metzger
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Publication number: 20170218427Abstract: The present invention relates to a method for detecting a viable microorganism in a pharmaceutical composition comprising the steps of providing a filterable pharmaceutical composition; filtering the pharmaceutical composition to provide at least three membranes upon which the pharmaceutical composition is deposited, placing the three membranes onto solid culture media to produce at least three filtrand cultures, culturing under aerobic and anaerobic conditions and detecting a viable microorganism cell, micro-colony or colony, wherein the presence of a viable cell, micro-colony or colony on the membrane indicates the presence of a viable microorganism in the pharmaceutical composition.Type: ApplicationFiled: December 1, 2016Publication date: August 3, 2017Inventors: Jennifer Claire ISKEN, Alexandra STAERK, Manfred BERCHTOLD
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Publication number: 20170218428Abstract: Spore carriers suitable for use in biological indicators are described. The spore carriers include a substrate such as polymeric film or non-woven web with a hydrophilic nanostructured layer bonded to it. Spores are bonded to the nanostructured layer. Nanostructured layers including nanoparticles, such as acid-sintered silica nanoparticles are described. Biological indicators including such spore carriers are also described.Type: ApplicationFiled: April 11, 2017Publication date: August 3, 2017Inventors: KELVIN J. WITCHER, WILLIAM E. FOLTZ, NAIYONG JING
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Publication number: 20170218429Abstract: Provided is a blood culture treatment technology, including a novel treatment technology of performing pretreatment of a positive blood culture to effectively remove foreign substances such as cells, etc., thereby omitting a subculturing process on a solid medium and realizing rapid selection of antimicrobial agents for patients. The pretreatment method of a positive blood culture sample for bacterial identification and antimicrobial susceptibility testing includes preparing a first liquid culture by centrifuging a portion of a liquid medium including the positive blood culture sample, washing a precipitate thus centrifuged with a solution including sodium chloride, and then suspending the precipitate in the solution including sodium chloride, and preparing a second liquid culture by passing the first liquid culture thus prepared through a pretreatment filter including a mesh structure.Type: ApplicationFiled: August 12, 2016Publication date: August 3, 2017Inventors: Yeon Joon PARK, Kang Gyun PARK
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Publication number: 20170218430Abstract: Provided are methods and compositions for determining methylarginine demethylase activity in test samples. The methods and compositions comprise a peptide substrate containing methylated arginine that can act as a substrate for the demethylation activity, a positive control that has methylarginine demethylation activity and a variant of the positive control that does not have methylarginine demethylation activity and that can act as a negative control.Type: ApplicationFiled: July 31, 2015Publication date: August 3, 2017Inventors: John M. Aletta, John C. Fisk, Laurie K. Read
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Publication number: 20170218431Abstract: A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample.Type: ApplicationFiled: April 12, 2017Publication date: August 3, 2017Applicants: GEN-PROBE INCORPORATED, QUALIGEN, INC.Inventors: Scott S. Breidenthal, Richard S. Lee, Norman C. Nelson, Matthew J. Scott, Jason A. Taylor
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Publication number: 20170218432Abstract: A random access, high-throughput system and method for preparing a biological sample for polymerase chain reaction (PCR) testing are disclosed. The system includes a nucleic acid isolation/purification apparatus and a PCR apparatus. The nucleic acid isolation/purification apparatus magnetically captures nucleic acid (NA) solids from the biological sample and then suspends the NA in elution buffer solution. The PCR testing apparatus provides multiple cycles of the denaturing, annealing, and elongating thermal cycles. More particularly, the PCR testing apparatus includes a multi-vessel thermal cycler array that has a plurality of single-vessel thermal cyclers that is each individually-thermally-controllable so that adjacent single-vessel thermal cyclers can be heated or cooled to different temperatures corresponding to the different thermal cycles of the respective PCR testing process.Type: ApplicationFiled: April 19, 2017Publication date: August 3, 2017Applicant: Siemens Healthcare Diagnostics Inc.Inventors: Robert Adolfsen, Nicolae Dumitrescu, Michael Avdenko, Dario Svenjak
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PCR AMPLIFICATION METHODS FOR DETECTING AND QUANTIFYING SULFATE-REDUCING BACTERIA IN OILFIELD FLUIDS
Publication number: 20170218433Abstract: At least one nucleic acid from a sulphate-reducing bacteria may be extracted from an oilfield fluid and may be amplified by a PCR amplification method in the presence of at least one primer to form an amplification product. The primer(s) may be or include a sequence essentially identical to SEQ ID NO:1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, and mixtures thereof. The amplification product may be hybridized with a probe specific for a fragment of an alpha subunit of an APS gene, and a presence of hybridization and a degree of hybridization may be detected.Type: ApplicationFiled: March 10, 2016Publication date: August 3, 2017Applicant: BAKER HUGHES INCORPORATEDInventors: Crystal Lee, Neil Sharma, Angela Reeves -
Publication number: 20170218434Abstract: The present disclosure provides for compositions of ?PNA probes. Additionally, the present disclosure provide for methods and kits using ?PNA probes for the diagnosis of sepsis.Type: ApplicationFiled: April 14, 2017Publication date: August 3, 2017Inventor: Alon Singer
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Publication number: 20170218435Abstract: Methods for selecting tag-oligonucleotide sequences for use in an in vitro nucleic acid assay. The selected tag sequences are useful for nucleic acid assay wherein interference between the nucleic acid sequences is the assay is to be controlled. Selected tag sequences are incorporated into nucleic acid assay to improve the performance of and/or minimize any interference between nucleic acids in the assay compared to untagged assays.Type: ApplicationFiled: November 28, 2016Publication date: August 3, 2017Inventors: Norman C. Nelson, Jijumon Chelliserry
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Publication number: 20170218436Abstract: A device for analyzing a liquid sample. The device includes an inlet for receiving the sample, a reaction chamber, an analysis module, and at least one pump for moving fluid within the one or more flow paths. The device includes one or more flow paths arranged so as to provide a fluid flow path between the inlet and the reaction chamber, and a fluid flow path between the reaction chamber and the analysis module. The device may be used for analyzing a liquid sample, such as, but not limited to nipple aspirate fluid (NAF).Type: ApplicationFiled: July 24, 2015Publication date: August 3, 2017Inventors: Mehdi Azimi, Matthew Taylor Worsman, Sara Alavioon, Simone Charlotte Vonwiller
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Publication number: 20170218437Abstract: Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including: methods of probe and target “engineering”, as well as methods of assay signal analysis relating to the modulation of the probe-target affinity constant, K by a variety of factors including the elastic properties of target strands and layers of immobilized (“grafted”) probes; and assay methodologies relating to: the tuning of assay signal intensities including dynamic range compression and on-chip signal amplification; the combination of hybridization-mediated and elongation-mediated detection for the quantitative determination of abundance of messages displaying a high degree of sequence similarity, including, for example, the simultaneous determination of the relative expression levels, and identification of the specific class of, untranslated AU-rich subsequences located near the 3? terminus of mRNA; and a new method of subtractive differential gene expression analysis which requires only a single color label.Type: ApplicationFiled: April 19, 2017Publication date: August 3, 2017Inventors: Michael SEUL, Sukanta Banerjee, Jiacheng YANG, Tatiana Vener
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Publication number: 20170218438Abstract: The invention provides a more efficient and less error-prone method of performing LAMP. The invention also provides a method for utilizing an RNase H2-cleavable probe as a technique for generating signal from the reaction, potentially increasing the specificity of the signal generation.Type: ApplicationFiled: February 1, 2017Publication date: August 3, 2017Applicant: Integrated DNA Technologies, Inc.Inventors: Joseph Dobosy, Aurita Menezes, Caifu Chen, Mark Behlke
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Publication number: 20170218439Abstract: The invention also encompasses novel structures and methods comprising providing a molecular adapter for capture and manipulation of transfer RNA. The adaptor is bound to a tRNA molecule. The adaptor may be a cholesterol-linked DNA adapter oligonucleotide. The invention is useful in sequencing, identification, manipulation and modification of tRNA.Type: ApplicationFiled: March 24, 2015Publication date: August 3, 2017Applicant: The Regents of the University of CaliforniaInventors: David Bernick, Andrew Smith
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Publication number: 20170218440Abstract: Methods, apparatus, and processes which use Extreme ultraviolet radiation (EUV) and/or soft X-ray wavelengths to read, image, edit, locate, identify, map, alter, delete, repair and sequence genes are described. An EUV scanning tool which allows high throughput genomic scanning of DNA, RNA and protein sequences is also described. A database which records characteristic absorption spectra of gene sequences is also described.Type: ApplicationFiled: February 1, 2017Publication date: August 3, 2017Inventor: Supriya JAISWAL
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Publication number: 20170218441Abstract: The present invention relates generally to methods of sequencing of polynucleotides and compounds, compositions and kits useful for sequencing of polynucleotides. The chemical compounds include nucleotide and nucleoside analogs which possess a blocking group covalently attached to the 3? hydroxyl of the sugar moiety. The blocking group may optionally be additionally covalently attached to a linker and/or detectable label. The nucleotide analogs may be ribonucleotide or deoxyribonucleotide analogs. Methods include incorporation of the reversible terminator molecules into growing polynucleotide strands by polymerase enzymes, such as in single base sequencing methodologies utilizing sequential reversible termination techniques.Type: ApplicationFiled: April 6, 2017Publication date: August 3, 2017Applicant: CENTRILLION TECHNOLOGY HOLDINGS CORPORATIONInventors: Wei ZHOU, Glenn MCGALL, Moti JAIN, Rui MEI
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Publication number: 20170218442Abstract: Provided herein are devices, systems, and methods of employing the same for the performance of bioinformatics analysis. The apparatuses and methods of the disclosure are directed in part to large scale graphene FET sensors, arrays, and integrated circuits employing the same for analyte measurements. The present GFET sensors, arrays, and integrated circuits may be fabricated using conventional CMOS processing techniques based on improved GFET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense GFET sensor based arrays. Improved fabrication techniques employing graphene as a reaction layer provide for rapid data acquisition from small sensors to large and dense arrays of sensors. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes, including DNA hybridization and/or sequencing reactions.Type: ApplicationFiled: April 10, 2017Publication date: August 3, 2017Inventors: Pieter van Rooyen, Mitchell Lerner, Paul Hoffman
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Publication number: 20170218443Abstract: Provided herein are Mycobacterium smegmatis porin nanopores, systems that comprise these nanopores, and methods of using and making these nanopores. Such nanopores may be wild-type MspA porins, mutant MspA porins, wild-type MspA paralog porins, wild-type MspA homolog porins, mutant MspA paralog porins, mutant MspA homolog porins, or single-chain Msp porins. Also provided are bacterial strains capable of inducible Msp porin expression.Type: ApplicationFiled: April 14, 2017Publication date: August 3, 2017Applicants: University of Washington, The UAB Research FoundationInventors: Jens H. Gundlach, Michael Niederweis, Thomas Z. Butler, Mikhail Pavlenok, Mark A. Troll, Suja Sukumaran
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Publication number: 20170218444Abstract: Methods for analyzing chromosomal DNA, including chromatin, are provided.Type: ApplicationFiled: February 8, 2017Publication date: August 3, 2017Applicant: Bio-Rad Laboratories, Inc.Inventors: Steven OKINO, Yan WANG
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Publication number: 20170218445Abstract: The present disclosure relates to a method for discovering effective bioactive compounds, based on plant developmental biology, and aims to discover a lead bioactive compound for new drugs by repeating screening based on phenotypes of a plant as a marker on the basis of plant developmental biology. To this end, the present disclosure provides a method for discovering effective bioactive compounds, and includes screening candidates for developing a new drug by using unique phenotypes as a marker shown by a plant when it is grown with a specific material.Type: ApplicationFiled: June 17, 2015Publication date: August 3, 2017Applicant: SEOUL NATIONAL UNIVERSITY R&DB FOUNDATIONInventors: Sunghwa CHOE, Maya Maharjan PUNA, Slki PARK, Richard CHA
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Publication number: 20170218446Abstract: The present invention concerns the finding that non-coding RNA profiles can be exploited as a means of monitoring, assessing, comparing, establishing and/or determining certain cell characteristics and/or profiles. Accordingly, the invention provides the use of non-coding RNA molecules for characterising and/or profiling cells.Type: ApplicationFiled: August 22, 2016Publication date: August 3, 2017Inventors: Vincent O'BRIEN, Chris HILLIER
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Publication number: 20170218447Abstract: The present invention provides methods for analyzing blocks of closely spaced SNPs, or haplotypes for use in identification of the origin of DNA in a sample. The methods comprise aligning common alleles of a gene of interest and identifying a region containing a plurality of SNPs which is flanked by non-polymorphic DNA which can be used for primer placement. Any sequencing method, including next generation sequencing methods can then be used to determine the haplotypes in the sample with a lower limit of detection of at least 0.01%. These inventive methods are useful, for example, for identification of hematopoietic stem cell transplantation patients destined to relapse, microchimerism associated with solid organ transplantation, detection of solid organ transplant rejection by detecting donor DNA in recipient plasma, forensic applications, and patient identification.Type: ApplicationFiled: August 5, 2015Publication date: August 3, 2017Inventors: James R. Eshleman, Sarah J. Wheelan, Jonathan Pevsner
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Publication number: 20170218448Abstract: Response to treatment of an inflammatory condition can be predicted based on characteristics of one or more markers from a subject. The markers can include expressions of nucleotide sequences identified herein and of combinations thereof. A response value can be calculated based on characteristics (e.g., expression levels) of one or more of the markers, as well as other characteristics of the subject, such as baseline clinical data. The treatment can be administered when the response value is beyond a threshold.Type: ApplicationFiled: January 29, 2016Publication date: August 3, 2017Applicant: BluePrint Bio, Inc.Inventors: Patrick LILLEY, Matthew NUNEZ
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Publication number: 20170218449Abstract: The present invention relates to an in vitro method for identifying agents capable of inducing sensitization of human skin and arrays and diagnostic kits for use in such methods. In particular, the methods include measurement of the expression of the biomarkers listed in Table 3A and/or 3B in MUTZ-3 cells exposed to a test agent.Type: ApplicationFiled: March 22, 2017Publication date: August 3, 2017Inventors: Malin Lindstedt, Carl Arne Krister Borrebaeck, Henrik Johansson, Ann-Sofie Albrekt
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Publication number: 20170218450Abstract: Methods, systems, and apparatus determine whether a first chromosomal region exhibits a deletion or an amplification associated with cancer in a sample from a subject (e.g., where the sample includes a mixture of cell-free DNA from tumor cells and non-malignant cells. Nucleic acid molecules of the biological sample are sequenced. Respective amounts of a clinically-relevant chromosomal region and of background chromosomal region(s) are determined from results of the sequencing. A parameter derived from these amounts (e.g. a ratio) is compared to one or more cutoff values, thereby determining a classification of whether first chromosomal region exhibits a deletion or an amplification associated with cancer.Type: ApplicationFiled: March 30, 2017Publication date: August 3, 2017Inventors: Yuk-Ming Dennis Lo, Rossa Wai Kwun Chiu, Kwan Chee Chan
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Publication number: 20170218451Abstract: Methods of treating, or at least inhibiting the onset of, urate transport failure are provided. The methods can include a step for detecting variations in genes that encode ABCG2 protein. When a subject has an SNP of V12M, R113X, Q126X, Q141K, F208S, G268R, E334X, S441N, L447V, S486N, F506SfsX4, R575X, and/or C608X, it can be concluded that the subject has a factor that is capable of inducing urate transport failure, or a state or disease attributable to that failure. When a subject has an SNP of V12M, it can be concluded that, unlike the other SNPs, there is a possibility that the subject does not possess such a factor because, although this variation itself does not lead to a change in urate transport capability, said variation is related to linkage disequilibrium with other SNPs.Type: ApplicationFiled: April 13, 2017Publication date: August 3, 2017Inventors: HIROTAKA MATSUO, Nariyoshi Shinomiya, Takahiro Nakamura, Tappei Takada, Hiroshi Suzuki, Yuki Ikebuchi, Kousei Ito, Kimiyoshi Ichida
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Publication number: 20170218452Abstract: The present invention provides novel mutations identified in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that can be used for a more accurate diagnosis of cystic fibrosis (CF) and CF related disorders. Methods for testing a sample obtained from a subject to determine the presence of one or more mutations in the CFTR gene are provided wherein the presence of one or more mutations indicates that the subject has CF or a CF related disorder, or is a carrier of a CFTR mutation.Type: ApplicationFiled: April 19, 2017Publication date: August 3, 2017Applicant: Laboratory Corporation of America HoldingsInventors: Elizabeth Rohlfs, Deborah Alexa Sirko-Osadsa, Lynn Rosenblum, Narasimhan Nagan, Zhaoqing Zhou, Ruth Heim
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Publication number: 20170218453Abstract: Provided herein are methods of adjusting or selecting a gluten peptide therapy based on the human leukocyte antigen (HLA) genotype, in particular HLA-DQ2.5 homozygosity, of a subject having or suspected of having Celiac disease. Also provided herein are methods of identifying (e.g., diagnosing) a subject, such as a subject having or suspected of having Celiac disease and/or assessing the efficacy of treatment of Celiac disease, e.g. by determining responsiveness to a therapeutic gluten peptide composition or cytokine response, and kits relating thereto.Type: ApplicationFiled: September 29, 2015Publication date: August 3, 2017Applicant: ImmusanT, Inc.Inventor: Robert P. Anderson
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Publication number: 20170218454Abstract: The invention encompasses products and methods relating to microRNAs involved in various cancers.Type: ApplicationFiled: June 26, 2014Publication date: August 3, 2017Inventors: Christopher L. Plaisier, Nitin S. Baliga
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Publication number: 20170218455Abstract: Provided herein are devices and methods for rapid analysis of biological samples. In particular, devices and methods described herein can be applied to rapid nucleic acid analysis of solid tissue samples.Type: ApplicationFiled: June 18, 2015Publication date: August 3, 2017Inventor: Brandon STEELMAN
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Publication number: 20170218456Abstract: Methods, systems, devices and computer implemented methods of prognosing or classifying patients using a biomarker comprising a plurality of subnetwork modules are disclosed. In some embodiments, the method comprises determining an activity of a plurality of genes in a test sample of a patient, wherein the plurality of genes are associated with the plurality of subnetwork modules. An expression profile is constructed using the activity of the plurality of genes. The dysregulation of each of the plurality of subnetwork modules is determined by calculating a score proportional to a degree of dysregulation in each of the plurality of subnetwork modules from the expression profile. The patient is prognosed or classified by inputting each dysregulation score into a model for predicting patient outcomes for patients having a disease, and inputting a clinical indicator of the patient into the model, to obtain a risk associated with the disease.Type: ApplicationFiled: July 23, 2015Publication date: August 3, 2017Inventors: John Bartlett, Paul Boutros, Victoria Sabine, Syed Haider, Maud H.W. Starmans, Cindy Qianli Yao, Jianxin Wang
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Publication number: 20170218457Abstract: The disclosure provides a correlation between the expression level of the miR-193a gene, which can be regulated by its methylation status, and both tumorigenesis of and the resistance of a cancer cell to a pyrimidine antimetabolite (5-FU) based chemotherapy. In addition to the methylation status and the expression of miR-193a, its downstream genes, such as E2F1, SRSF2, and apoptotic genes such as caspase 2, are also involved and can serve as useful markers for cancer therapy prognosis and for therapy selection.Type: ApplicationFiled: December 2, 2016Publication date: August 3, 2017Inventor: Jingde Zhu
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Publication number: 20170218458Abstract: The present application discloses a method of detecting the methylation of specific genome regions in a DNA fragment from a sample containing DNA. The method includes treating the DNA fragment containing specific genome regions with sodium bisulfite and obtaining single-strand DNA fragment; attaching an adapter to one or both ends of the single-strand DNA fragment; optionally cyclizing the adapter-attached single-strand DNA fragment; preparing the single-strand DNA fragment with attached adapter into a DNA sequencing library containing the specific genome regions; sequencing the DNA sequencing library to identify the sequence of the single-strand DNA fragment. The present application also discloses a kit for detecting the methylation of specific genome regions in a DNA fragment from a sample containing DNA, and the use of single strand ligating agent in preparing a kit for detecting the methylation of specific genome regions in a DNA fragment.Type: ApplicationFiled: February 3, 2017Publication date: August 3, 2017Inventors: Jian-Bing FAN, Xuyu CAI, Yangbin GAO
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Publication number: 20170218459Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.Type: ApplicationFiled: March 23, 2017Publication date: August 3, 2017Inventors: AmirAli TALASAZ, Helmy ELTOUKHY
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Publication number: 20170218460Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.Type: ApplicationFiled: April 20, 2017Publication date: August 3, 2017Inventor: AmirAli TALASAZ
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Publication number: 20170218461Abstract: The present invention relates to the field of biotechnology, in particular to genes and use thereof. The present invention employs whole genome sequencing to perform whole genome re-sequencing on a large number of individuals of the honey bee Apis mellifera sinisxinyuan, and obtains genes specific to the A. m. sinisxinyuan. The genes play important roles in the differentiation of A. m. sinisxinyuan from the honey bees in other regions and in the adaptive evolution of A. m. sinisxinyuan to the local environment. The Foxo gene or the Ebony gene provided in the present invention can be used to identify A. m. sinisxinyuan from other subspecies; can also be used for studying the genetic diversity of species resources of bees; and can further be used for studying cold resistance genes. This will fill the gap in the research field of A. m. sinisxinyuan by Chinese researchers.Type: ApplicationFiled: January 23, 2017Publication date: August 3, 2017Applicant: INSTITUTE OF APICULTURAL RESEARCH, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Chao CHEN, Wei SHI, Zhiguang LIU, Xiao CHEN, Huihua WANG, Haikun GUO
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Publication number: 20170218462Abstract: The present invention relates to the field of biotechnology, in particular to genes and use thereof. The present invention employs whole genome sequencing to perform whole genome re-sequencing on a large number of individuals of the honey bee Apis mellifera sinisxinyuan, and obtains genes specific to A. m. sinisxinyuan. The genes play important roles in the differentiation of A. m. sinisxinyuan from the honey bees in other regions and in the adaptive evolution of A. m. sinisxinyuan to the local environment. The FilI gene or the Ds gene provided in the present invention can be used to identify A. m. sinisxinyuan from other subspecies; can also be used for studying the genetic diversity of species resources of bees; and can further be used for studying stress resistance genes. This will fill the gap in the research field of A. m. sinisxinyuan by Chinese researchers.Type: ApplicationFiled: January 23, 2017Publication date: August 3, 2017Applicant: INSTITUTE OF APICULTURAL RESEARCH, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Zhiguang LIU, Wei SHI, Chao CHEN, Xiao CHEN, Haikun GUO, Huihua WANG
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Publication number: 20170218463Abstract: A computer-implemented method of preparing a set of differentially informative methylated positions (DIMPs) or differentially informative methylated regions (DIMRs) from a sample methylome of an animal or plant having a phenotypic characteristic different from a wild-type of the same species of animal or plant, and the characteristic is associate with differences in methylation of the genome, comprises: providing a computer with the sample methylome, and a reference methylome of the wild-type of the same species of animal or plant; calculating with the computer a divergence between a plurality of cytosine positions of the sample methylome and the reference methylome; and selecting with the computer a set of DIMPs or DIMRs. Each DIMP or DIMR is selected based on an approximation of the energy required to produce the divergence between methylation levels of the plurality of cytosine positions of the sample methylome as compared to the wild-type methylome.Type: ApplicationFiled: February 1, 2017Publication date: August 3, 2017Inventors: Sally A. Mackenzie, Robersy Sanchez
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Publication number: 20170218464Abstract: The presently disclosed subject matter provides methods, reporter gene constructs, and kits for using prostate-specific membrane antigen (PSMA) as an imaging reporter to image a variety of cells and tissues.Type: ApplicationFiled: March 18, 2015Publication date: August 3, 2017Applicant: THE JOHNS HOPKINS UNIVERSITYInventors: MARTIN G. POMPER, MARK CASTANARES, IL MINN, SHAWN LUPOLD
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Publication number: 20170218465Abstract: The present disclosure provides a composition comprising a panel of probes for detecting one or more viruses in a sample. The panel of probes may be used to detect viruses in a biological sample obtained from a subject.Type: ApplicationFiled: January 27, 2017Publication date: August 3, 2017Applicant: Washington UniversityInventors: Gregory Storch, Todd Wylie, Kristine Wylie
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Publication number: 20170218466Abstract: The invention concerns the quantitative analysis of circulating JCPyV microRNAs (miRNA) in body fluid and its use as biomarker for JCPyV infection.Type: ApplicationFiled: July 30, 2015Publication date: August 3, 2017Inventors: Ole Siegfrid Lagatie, Lieven Jozef Stuyver
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Publication number: 20170218467Abstract: The present invention relates to a method for detecting or quantifying Human Immunodeficiency Virus-2 (HIV-2) nucleic acids in a biological sample, comprising: a) performing a real-time polymerase chain reaction (PCR) or a real-time reverse transcriptase polymerase chain reaction (RT-PCR) on nucleic acids of the biological sample with: (i) at least 4 primers respectively comprising or consisting of: —sequence SEQ ID NO: 1 or a sequence having at least 90% identity to SEQ ID NO: 1, a nd—sequence SEQ ID NO: 2 or a sequence having at least 90% identity to SEQ ID NO: 2, a nd—sequence SEQ ID NO: 4 or a sequence having at least 90% identity to SEQ ID NO: 4, a nd—sequence SEQ ID NO: 5 or a sequence having at least 90% identity to SEQ ID NO: 5, a nd (ii) at least 2 labelled probes respectively comprising or consisting of:—sequence SEQ ID NO: 3, a sequence complementary to SEQ ID NO: 3, or a sequence having at least 90% identity to SEQ ID NO: 3 or the complementary thereof, and—sequence SEQ ID NO: 6, a sequence compleType: ApplicationFiled: May 27, 2015Publication date: August 3, 2017Inventors: Christine ROUZIOUX, Jean-Christophe PLANTIER, Véronique AVETAND-FENOEL, Florence DAMOND, Marie GUEDIN, Diane DESCAMPS
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Publication number: 20170218468Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.Type: ApplicationFiled: February 20, 2015Publication date: August 3, 2017Inventors: Roelof Hendrik Matthijs KOUWEN, Pim VAN HEE, Douwe VAN SINDEREN, Brian MCDONNELL, Jennifer MAHONY
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Publication number: 20170218469Abstract: The method employs a desulfurization agent that is introduced into a smelt of one of molten pig iron and molten steel. The desulfurization agent contains calcium oxide, bitumen and at least one flux agent, with the agent containing 1 to 10% by weight bitumen.Type: ApplicationFiled: April 20, 2017Publication date: August 3, 2017Applicant: Almanet GMBHInventor: Frank Herwig
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Publication number: 20170218470Abstract: A burner-lance unit (1) includes at least two gas connections (2a, 2b, 2c), a burner tube (3), and a lance tube (4) that is placed concentrically in the burner tube (3). The burner tube (3) and the lance tube (4) both have a gas inlet end and a gas outlet end (15). The lance tube (4) has a de Laval nozzle (4a) at the gas outlet end thereof. The de Laval nozzle (4a) is releasably connected to the lance tube (4). The burner tube (3) has a burner nozzle (3a) which is releasably connected to the burner tube (3).Type: ApplicationFiled: August 4, 2015Publication date: August 3, 2017Applicant: Primetals Technologies Austria GmbHInventors: Markus ABEL, Hannes BEILE, Markus DORNDORF, Alexander MÜLLER, Ralf PITZ
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Publication number: 20170218471Abstract: A cold-rolled flat steel product may have a yield strength Rp0.2 of not more than 320 MPa, a fracture elongation A80 of at least 20% and a microstructure having by percent area 62%-82% ferrite, 10%-30% martensite, 1.5%-8% residual austenite, and a sum total of not more than 10% other microstructure constituents. The flat steel product may comprise a steel alloy containing in percent by weight 0.06%-0.1% C; 0.15%-0.4% Si; 1.5%-2% Mn; 0.2%-0.5% Cr; not more than 0.1% Al; wherein the sum total of C, Si, Mn, and Cr is at least 2.3% and not more than 2.8%; wherein the sum total of Si and Al is not more than 0.4%; not more than 0.03% P; not more than 0.006% S; not more than 0.008% N; unavoidable impurities including not more than 0.0006% B, not more than 0.02% V, and not more than 0.01% each of Nb and Ti, and not more than 0.1% each of Mo, Ni, and Cu; as well as iron. For production of such a flat steel product, a cold-rolled flat steel product may first be produced and then brought to 760-860° C.Type: ApplicationFiled: March 18, 2015Publication date: August 3, 2017Applicants: ThyssenKrupp Steel Europe AG, ThyssenKrupp AGInventors: Andreas Bongards, Sigrun Voß, Roland Sebald
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Publication number: 20170218472Abstract: Disclosed is a high-strength steel sheet having a tensile strength (TS) of 780 MPa or more and excellent in ductility, fatigue properties, stretch flangeability, surface characteristics, and sheet passage ability that can be obtained by providing a predetermined chemical composition and a steel microstructure that contains, by area, 20-50% of ferrite, 5-25% of bainitic ferrite, 1-10% of martensite, and 5-15% of tempered martensite, and that contains, by volume, 10% or more of retained austenite, in which the retained austenite has a mean grain size of 2 ?m or less, a mean Mn content in the retained austenite in mass % is at least 1.2 times the Mn content in the steel sheet in mass %, the retained austenite has a mean free path of 1.2 ?m or less, and the tempered martensite has a mean free path of 1.2 ?m or less.Type: ApplicationFiled: August 5, 2015Publication date: August 3, 2017Applicant: JFE STEEL CORPORATIONInventors: Yoshiyasu KAWASAKI, Hiroshi MATSUDA, Kazunori TAHARA, Takeshi YOKOTA, Kaneharu OKUDA, Kazuhiro SETO
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Publication number: 20170218473Abstract: A method for heating a blank or a preformed steel sheet component for hot forming and/or quench hardening purposes. In at least some regions, the heating is carried out to a temperature above AC3; the heating of the Hank is embodied as a rapid heating and to this end, the blank is heated in a first zone at an average heating rate of >25 K/s up to about 600° C. and above this temperature, is heated at an average heating rate of >10 K/s up to a maximum of the AC3 temperature and then is transferred to a second zone in which the blank that has been preheated in the first zone is heated in at least some regions to temperatures greater than AC3, in particular >850° C., with the heating rate in the second zone being >10 K/s. The invention also relates to a device for carrying out the method.Type: ApplicationFiled: July 21, 2015Publication date: August 3, 2017Inventors: Michael Haslmayr, Siegfried Kolnberger, Thomas Kurz, Leopold Stegfellner, Andreas Sommer
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Publication number: 20170218474Abstract: A method of manufacturing a high-strength hot rolled steel sheet by hot rolling a steel having a chemical composition including, by mass %: C: more than 0.010% and not more than 0.06%, Si: not more than 0.3%, Mn: not more than 0.8%, P: not more than 0.03%, S: not more than 0.02%, Al: not more than 0.1%, N: not more than 0.01% and Ti: 0.05 to 0.10%, the balance including Fe and inevitable impurities, including: after the steel is heated to an austenite single phase region, the steel is finish rolled at a finishing delivery temperature of 860° C. to 1050° C., the steel sheet is cooled at an average cooling rate of not less than 30° C/s in a temperature range of from a temperature after the completion of the finish rolling to 750° C., and the steel sheet is coiled into a coil at a coiling temperature of 580° C. to 700° C.Type: ApplicationFiled: April 11, 2017Publication date: August 3, 2017Inventors: Yoshimasa Funakawa, Tetsuo Yamamoto, Hiroshi Uchomae, Hiroshi Nakano, Taro Kizu
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Publication number: 20170218475Abstract: Disclosed is a method comprising: preparing a steel slab with a predetermined chemical composition; subjecting the steel slab to hot rolling by heating it to a temperature of 1100-1300° C., hot rolling it with a finisher delivery temperature of 800-1000° C. to form a hot-rolled steel sheet, and coiling the steel sheet at a mean coiling temperature of 200-500° C.; subjecting the steel sheet to pickling treatment; and subjecting the steel sheet to annealing by retaining the steel sheet at a temperature of 740-840° C. for 10-900 s, then cooling the steel sheet at a mean cooling rate of 5-50° C./s to a cooling stop temperature of higher than 350° C. and 550° C. or lower, and retaining the steel sheet in a temperature range of higher than 350° C. to 550° C. for 10 s or more.Type: ApplicationFiled: August 5, 2015Publication date: August 3, 2017Applicant: JFE STEEL CORPORATIONInventors: Yoshiyasu KAWASAKI, Hiroshi MATSUDA, Takeshi YOKOTA, Yoshimasa FUNAKAWA, Kazuhiro SETO, Yukihiro MATSUBARA