Patents Issued in November 2, 2017
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Publication number: 20170314031Abstract: The present invention relates to an optimized metabolic pathway design in P. pastoris. In particular, to a recombinant polycistronic expression construct for stable expression of multiple genes of interest in a yeast cell, preferably in P. pastoris.Type: ApplicationFiled: October 28, 2015Publication date: November 2, 2017Applicant: ACIB GmbHInventors: Martina GEIER, Pia FAULAND, Anton GLIEDER
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Publication number: 20170314032Abstract: A method is provided for transforming monocotyledonous plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of monocot plant tissue with heterologous DNA constructs. The invention also provides for monocot cells in which the plastids contain heterologous DNA constructs.Type: ApplicationFiled: June 9, 2017Publication date: November 2, 2017Inventors: Vladimir Sidorov, Jeffrey M. Staub, Yuechun Wan, Guangning Ye
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Publication number: 20170314033Abstract: The invention provides the genetically manipulated herbicide-resistant rubber producing dandelion plants and seed of said plants. Another aspect of the invention comprises progeny plants, or seeds, or regenerable parts of plants and seeds of the genetically manipulated herbicide-resistant dandelion plants. Applicants have further found that use of root cells for transformation and other optimized protocols enable quick transformation with high plant regeneration. Further, Applicants have developed the first transformation/regeneration protocol that is successful without the addition of hormone treatment.Type: ApplicationFiled: April 25, 2017Publication date: November 2, 2017Inventors: Katrina Cornish, Kyle Arthur Benzle, Lu Zhao, Yingxiao Zhang, Brian Iaffaldano
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Publication number: 20170314034Abstract: A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes.Type: ApplicationFiled: June 6, 2017Publication date: November 2, 2017Inventors: Ann J. Slade, Dayna L. Loeffler, Aaron M. Holm, Jessica C. Mullenberg
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Publication number: 20170314035Abstract: A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes.Type: ApplicationFiled: July 13, 2017Publication date: November 2, 2017Inventors: Ann J. Slade, Dayna L. Loeffler, Aaron M. Holm, Jessica C. Mullenberg
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Publication number: 20170314036Abstract: The invention provides methods and materials for increasing at least one of root biomass and above-ground biomass and in a Poaceae plant by expressing a PEAPOD protein, or fragment thereof, in the Poaceae plant. The invention also provides methods and materials producing a Poaceae plant with at least one of increased root biomass and increased above-ground biomass, by expressing a PEAPOD protein, or fragment thereof, in the Poaceae plant.Type: ApplicationFiled: November 3, 2015Publication date: November 2, 2017Applicant: Agresearch LimitedInventors: Nicholas John ROBERTS, Kim Archer RICHARDSON, Derek William Richard WHITE
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Publication number: 20170314037Abstract: This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.Type: ApplicationFiled: July 12, 2017Publication date: November 2, 2017Applicant: Monsanto Technology LLCInventors: David K. KOVALIC, Jack E. TABASKA, Bo-Xing QIU, Wei WU
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Publication number: 20170314038Abstract: Two small proteins with anti-bacterial activity are generated and called optimized thionin and optimized pro-thionin. Optimized pro-thionin contains a signal sequence for intracellular trafficking of the protein and is cleaved off to yield optimized thionin. Genetically altered plants and their progeny expressing a polynucleotide encoding optimized pro-thionin or optimized thionin resist diseases caused by bacteria.Type: ApplicationFiled: June 29, 2017Publication date: November 2, 2017Inventors: Eddie W Stover, Goutam Gupta, Guixia Hao
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Publication number: 20170314039Abstract: The present invention relates to a plant belonging to the Solanaceae family wherein said plant comprises a genetic trait providing Phytophthora resistance and wherein said resistance trait is encoded by a combination of at least two genes having a reduced expression, or transcription, of said genes or a reduced activity of proteins encoded by said genes as compared to said plant belonging to Solanaceae family being susceptible to Phytophthora. The present invention especially relates to plants belonging to the Solanaceae family selected from the group consisting of sweet pepper (Capsicum annuum), Capsicum spp., potato (Solanum tuberosum), petunia, tomato (Solanum lycopersicum), Nicotinia benthamiana, and tabacco (Nicotinia tabacum), and the present Phytophthora resistance is especially a resistance to a plant pathogen selected from the group consisting of Phytophthora spp., Phytophthora capsici, Phytophthora infestans, and Phytophthora nicotinae.Type: ApplicationFiled: June 18, 2015Publication date: November 2, 2017Applicant: Enza Zaden Beheer B.V.Inventors: Christianus Cornelis Nicolaas VAN SCHIE, Karin Ingeborg POSTHUMA, Tieme ZEILMAKER, Geert Johannes DE BOER
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Publication number: 20170314040Abstract: The present invention discloses RNAi constructs derived from a Fusarium chitin synthase gene Chs3b, which has a nucleotide sequence as shown by SEQ ID NO: 1. Five distinct RNAi vectors are constructed for 5 different segments of the Chs3b gene (named Chs3b-1, 2, 3, 4, and 5, respectively), and separately transformed into Fusarium. It is found that siRiNAs in the transformed Fusarium shows a significant inhibition in fungal growth, development, and pathogenicity. The expression of the RNAi vectors against Chs3b in plants may inhibit the infection of Fusarium and improve the resistance of the transgenic plants to Fusarium head blight.Type: ApplicationFiled: March 24, 2015Publication date: November 2, 2017Inventors: Yucai Liao, Heping Li, Xiushi Song, Wei Cheng, Peng Yang
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Publication number: 20170314041Abstract: The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3? ends such that the multimeric coding nucleic acid compound comprises two or more 5? ends.Type: ApplicationFiled: April 7, 2017Publication date: November 2, 2017Inventors: Frank DeRosa, Michael Heartlein, Daniel Crawford, Shrirang Karve
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Publication number: 20170314042Abstract: This disclosure relates to vectors, isolated cells, compositions, and methods for the treatment of critical limb ischemia and associated disorders. One aspect of the disclosure relates to a vector comprising a nucleic acid encoding a 165A isoform VEGF protein and a promoter that regulates expression of the nucleic acid encoding the VEGF.Type: ApplicationFiled: November 14, 2016Publication date: November 2, 2017Applicant: The Regents of the University of CaliforniaInventors: Jan Nolta, Karen Pepper, Fernando Fierro, Gerhard Bauer
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Publication number: 20170314043Abstract: The present disclosure generally relates to viral-based expression systems suitable for the production of molecule of interests in recombinant host cells. The disclosure particularly relates to nucleic acid constructs, such as expression vectors, containing a modified arterivirus genome or replicon RNA in which at least some of its original viral sequence has been deleted. Also included in the disclosure are viral-based expression vectors including one or more expression cassettes encoding heterologous polypeptides. In some embodiments, the expression cassettes are configured and positioned at defined locations on the viral genome so as to enable expression of the heterologous polypeptides in a tunable manner.Type: ApplicationFiled: April 12, 2017Publication date: November 2, 2017Inventors: Kurt Iver Kamrud, Nathaniel Stephen Wang, Martina Felderman, Nancy C. Carrico
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Publication number: 20170314044Abstract: This disclosure describes oncolytic adenovirus constructs and methods on using the constructs. Generally, the oncolytic constructs includes at least a partial deletion of E3, a heterologous polynucleotide encoding a heterologous polypeptide in place of the at least partial deletion of E3, and a cell-specific regulatory polynucleotide operationally linked to the heterologous polynucleotide.Type: ApplicationFiled: May 2, 2017Publication date: November 2, 2017Inventors: Julia Davydova, Masato Yamamoto, Lisa Koodie
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Publication number: 20170314045Abstract: The present invention relates to methods and apparatus for microbial conversion of carbon dioxide, carbon monoxide and hydrogen to methane, and to the microbial populations, and the media comprising the microbial populations, that may be used in such methods and apparatus.Type: ApplicationFiled: June 29, 2017Publication date: November 2, 2017Inventors: Sandra ESTEVES, Richard Mark DINSDALE, Timothy PATTERSON, Savvas SAVVAS
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Publication number: 20170314046Abstract: The present invention provides a method for treatment of biomass material by fermentation, said method comprises a pre-treatment of the biomass material by thermal hydrolysis and wet explosion, resulting in an intermediate product having a dry matter concentration above 25% and temperature above 90° C. which is to be introduced to the fermentation, wherein a part of the content of the digestion tank used for the fermentation is recirculated and mixed with a part of the intermediate product from the pre-treatment.Type: ApplicationFiled: October 29, 2015Publication date: November 2, 2017Applicant: CAMBI TECHNOLOGY ASInventors: Paal Jahre NILSEN, Hans Rasmus HOLTE
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Publication number: 20170314047Abstract: A method of producing biodiesel includes reacting at least one of brown grease and FOG with at least one enzyme, alcohol, and an aqueous solution to produce a reacted feedstock and producing biodiesel from the reacted feedstock, the biodiesel having a composition of sulfur less than 15 ppm.Type: ApplicationFiled: May 19, 2015Publication date: November 2, 2017Inventors: Stuart LAMB, Franklin MATHIS, Brent CHRABAS
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Publication number: 20170314048Abstract: Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.Type: ApplicationFiled: February 27, 2017Publication date: November 2, 2017Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George N. Rudenko, Penelope Chua
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Publication number: 20170314049Abstract: The invention relates to recombinant microorganisms and methods for producing manoyl oxide.Type: ApplicationFiled: November 13, 2015Publication date: November 2, 2017Applicant: Evolva SAInventor: Niels Bjerg Jensen
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Publication number: 20170314050Abstract: A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.Type: ApplicationFiled: February 26, 2015Publication date: November 2, 2017Inventors: KEVIN D NAGY, Erwin Columbus Hago, Jayarama K Shetty, Susan Marie Hennessey, Robert Dicosimo, Ling Hua, Rodrigo Ramirez, Zhongmei Tang, Zheyong Yu
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Publication number: 20170314051Abstract: A method of producing a sugar liquid and a xylooligosaccharide includes Steps (1) to (3): Step (1): hydrolyzing a cellulose-containing biomass with a filamentous fungus-derived cellulase; Step (2): subjecting the hydrolysate of Step (1) to solid-liquid separation, and filtering the solution component through an ultrafiltration membrane to recover cellulase as a non-permeate, and to recover a sugar liquid as a permeate; and Step (3): reacting the recovered cellulase in Step (2) with a xylan-containing material, and recovering a xylooligosaccharide produced, Step (3) being independent from Step (1).Type: ApplicationFiled: October 29, 2015Publication date: November 2, 2017Inventors: Chiaki Yamada, Hiroyuki Kurihara, Katsushige Yamada
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Publication number: 20170314052Abstract: The present invention relates to the production of the disaccharide kojibiose which is known to be a powerful prebiotic. The invention indeed discloses the generation of genetically modified sucrose phosphorylases which convert—via a transglycosylation reaction—sucrose into kojibiose in a very efficient manner. Hence, the present invention relates to a cost-effective production method of kojibiose which is useful within industry.Type: ApplicationFiled: November 12, 2015Publication date: November 2, 2017Applicant: Universiteit GentInventors: Tom VERHAEGHE, Karel DE WINTER, Tom DESMET
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Publication number: 20170314053Abstract: A cassette sequence for the transformation of a host cell includes at least: a first nucleotide sequence encoding a peptide or protein of interest to be produced by the host cell. The first sequence is linked to a second nucleotide sequence providing resistance to a toxin or encoding an antitoxin peptide to the toxin. The nucleotide sequences are organized in such a way that production of the peptide encoded by the second nucleotide sequence(s) is translationally coupled to production of the peptide encoded by the first nucleotide sequence.Type: ApplicationFiled: July 6, 2015Publication date: November 2, 2017Applicant: UNIVERSITE LIBRE DE BRUXELLESInventors: Laurence VAN MELDEREN, Baptiste J. S. DUMONT, Thibaut HALLAERT, Gilles VANWALLEGHEM, Philippe GOFFIN
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Publication number: 20170314054Abstract: According to one aspect, the present disclosure provides a method of identifying a substrate of a transglutaminase using a peptide array comprising a plurality of peptides. The method includes the steps of contacting the peptides in the peptide array with the transglutaminase, allowing the transglutaminase to bind to the peptides, and identifying the substrate of the transglutaminase.Type: ApplicationFiled: June 16, 2017Publication date: November 2, 2017Inventors: THOMAS ALBERT, FRANK BERGMANN, VICTOR LYAMICHEV, JIGAR PATEL, MICHAEL SCHRAEML, WOJTEK STEFFEN
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Publication number: 20170314055Abstract: Herein is reported a method for producing an enzymatic conjugation product of three polypeptides comprising the simultaneous incubation of i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 20, wherein X can be any amino acid residue), a second polypeptide comprising the amino acid sequence LPXTA (SEQ ID NO: 31, wherein X can be any amino acid residue), a third polypeptide that has two N-termini whereby the polypeptide has an oligo-glycine Gm (m=2 (SEQ ID NO: 22), or 3 (SEQ ID NO: 23), or 4 (SEQ ID NO: 24), or 5 (SEQ ID NO: 25)) amino acid sequence at its first N-terminus and an oligo-alanine Am (m=2 (SEQ ID NO: 26), or 3 (SEQ ID NO: 27), or 4 (SEQ ID NO: 28), or 5 (SEQ ID NO: 29)) amino acid sequence at its second N-terminus, a fourth polypeptide with sortase activity whereby the polypeptide is derived from Staphylococcus aureus sortase A, and a fifth polypeptide with sortase activity whereby the polypeptide is derived from Streptococcus pyogenes sortase A and the recovering of the cType: ApplicationFiled: June 16, 2017Publication date: November 2, 2017Applicant: Hoffmann-La Roche Inc.Inventors: Dieter Heindl, Erhard Kopetzki, Georg Tiefenthaler
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Publication number: 20170314056Abstract: The present invention describes methods of identifying, detecting, imaging, isolating and locating cancer cells in a subject. The method involves the use of near-infrared (NIR) organic carbocyanine dyes, particularly, near infrared heptamethine cyanine dyes and the detection of the fluorescence of these NIR dyes. The uptake of these dyes by cancer cells and not by normal cells, as well as their high intensity, among other things, allow for the detection of cancerous cells in a subject and facilitate their subsequent isolation. Further, detection of many tumor types and tumor cell populations under cell culture and in vivo conditions are described.Type: ApplicationFiled: April 13, 2017Publication date: November 2, 2017Inventors: Xiaojian Yang, Haiyen E. Zhau, Ruoxiang Wang, Leland W.K. Chung
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Publication number: 20170314057Abstract: When bacteria growth is determined in the related art using an ATP method, the bacteria growth is determined, based on an increase or a decrease in live bacteria ATP with the lapse of a culture time. Accordingly, it is necessary to measure the live bacteria ATP multiple times while antimicrobial susceptibility culture is carried out. It takes time and labor in sample preparation for each measurement, and it is difficult to quickly obtain an antimicrobial susceptibility result. Therefore, according to the present invention, the presence or absence of antimicrobial susceptibility of bacteria is determined, based on an ATP luminescence amount derived from dead bacteria in a culture liquid.Type: ApplicationFiled: December 26, 2014Publication date: November 2, 2017Inventors: Hiroko TADA, Hideyuki NODA, Hideki NIIMI, Isao KITAJIMA
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Publication number: 20170314058Abstract: A method for rapid, high throughput screening of the activities of enzymes, especially ligninases and its enzyme cocktails, using nanostructure initiator mass spectrometry (NIMS) surfaces, substrates and methodology.Type: ApplicationFiled: April 27, 2017Publication date: November 2, 2017Inventors: Trent R. Northen, Kai Deng
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Publication number: 20170314059Abstract: The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase-producing microorganisms.Type: ApplicationFiled: July 17, 2017Publication date: November 2, 2017Applicant: 3M INNOVATIVE PROPERTIES COMPANYInventors: PATRICK A. MACH, MICHELLE L. ROSAUER, MICHAEL E. HUGHES
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Publication number: 20170314060Abstract: Methods of determining polymerase fidelity are provided. In one embodiment, the method comprises filling a gapped plasmid with a polymerase to form a gap-filled plasmid, wherein the gap-filled plasmid comprises a gene encoding an protein that is functional or non-functional depending on the polymerase fidelity; forming a plurality of partitions from a solution comprising the gap-filled plasmid and a label for detecting the presence of the plasmid; detecting the presence of the gap-filled plasmid in one or more of the partitions; and determining the fidelity of the polymerase by determining a ratio of partitions containing the gene encoding a functional protein to partitions containing a gene encoding a non-functional protein.Type: ApplicationFiled: April 28, 2017Publication date: November 2, 2017Inventors: Man CHENG, Xiangdong MENG, Jan ZUR MEGEDE
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Publication number: 20170314061Abstract: Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.Type: ApplicationFiled: July 10, 2017Publication date: November 2, 2017Inventors: Lance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
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Publication number: 20170314062Abstract: Methods and related products are disclosed that improve the probability of interaction between a target molecule and a nanopore by capturing the target molecule on a surface comprising the nanopore. The captured target molecule, the nanopore, or both, are able to move relative to each other along the surface. When the leader of the target molecule is in proximity with the nanopore, interaction of the target portion of the target molecule with the nanopore occurs, thereby permitting sensing of the target portion. Confining the target molecule and nanopore in this manner leads to significantly enhanced interaction with the nanopore.Type: ApplicationFiled: May 2, 2017Publication date: November 2, 2017Inventors: Mark Stamatios Kokoris, Robert N. McRuer
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Publication number: 20170314063Abstract: This invention relates to the production and genotyping of mice lacking both Angiopoietin 1 and Angiopoietin 2. This invention also relates to the use of Tie2 receptor activation for treatment of open angle glaucoma, congenital glaucoma and cystic kidney disease, and more specifically to the use of angiopoietin 1 recombinant proteins, peptides, VE-PTP phosphatase inhibitors, and Tie2-peptomimetics to improve lymphatic drainage in the Schlemm's canal and corneal limbal lymphatic system for open angle glaucoma and congenital glaucoma patients, and to slow and/or reduce the growth of cysts in patients with cystic kidney disease.Type: ApplicationFiled: June 23, 2017Publication date: November 2, 2017Inventor: Susan E. QUAGGIN
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Publication number: 20170314064Abstract: Provided are methods and systems for detecting formation of nucleotide-specific ternary complexes comprising a DNA polymerase, a nucleic acid, and a nucleotide complementary to the templated base of the primed template nucleic acid. The methods and systems facilitate determination of the next correct nucleotide without requiring chemical incorporation of the nucleotide into the primer. These results can even be achieved in procedures employing unlabeled, native nucleotides.Type: ApplicationFiled: April 28, 2017Publication date: November 2, 2017Applicant: Omniome, Inc.Inventors: Pinar Iyidogan, Kandaswamy Vijayan
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Publication number: 20170314065Abstract: The present invention is based on the discovery of novel polymorphisms (SNPs) in the penicillin binding protein (pbp3) gene in Staphylococcus aureus. The presence of G88A and/or G2047A SNPs provides an accurate, reliable biomarker for the presence of Methicillin Resistant Staphylococcus aureus (MRSA), specifically the community-associated MRSA (CA-MRSA). The present invention provides reagents used for detecting the SNPs as well as methods of identifying and using these variants to screen subjects for presence of CA-MRSA. The methods involve isolating a biological sample from a mammal (preferably a human) and testing for the presence of a SNP in the pbp3 gene which is associated with CA-MRSA.Type: ApplicationFiled: July 14, 2017Publication date: November 2, 2017Applicant: Medical Diagnostic Laboratories, LLCInventors: Scott E. Gygax, Sean Chadwick, Aditya Prasad, Martin E. Adelson, Eli Mordechai
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Publication number: 20170314066Abstract: A single-molecule mechanoanalytical real-time sensing device (SMART) comprising a molecular trawl and a DNA stem-loop structure that serves as a molecular dipstick, said trawl and said DNA stem-loop structure anchored by handles to two optically-trapped bead templates; said molecular trawl comprising multiple analyte recognition elements that exist in each of two separate DNA (pair) strands that act as two pincers, said pincers each having a nucleobase capable of catching an analyte in a media; said DNA stem-loop structure comprising a plurality of nucleotides in said loop and multiple base pairs in said stem; and wherein said DNA stem-loop is located generally opposite to said molecular trawl that is capable of reporting an amount of bound analyte target via mechanochemical transient events.Type: ApplicationFiled: April 20, 2017Publication date: November 2, 2017Inventors: Hanbin MAO, Shankar MANDAL
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Publication number: 20170314067Abstract: In accordance with some embodiments herein, compositions and methods for accurate barcoding of nucleic acids are described. The compositions and methods can involve a plurality of unique oligonucleotide species comprising unique molecule barcodes. In some embodiments, the molecule barcodes can have a relatively low G content, and can exhibit reduced bias in amplification and analysis.Type: ApplicationFiled: April 28, 2017Publication date: November 2, 2017Inventors: Eleen Shum, Glenn Fu
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Publication number: 20170314068Abstract: Disclosed herein are methods and compositions for determining the copy number of a first target nucleic acid as compared to the copy number of a second target nucleic acid in a single well with a single detection label. For example, disclosed herein are methods and compositions for determining the copy number of a first target nucleic acid as compared to the copy number of a second target nucleic acid by a monochrome multiplex quantitative PCR (MMQPCR) in a single well with a single detection label.Type: ApplicationFiled: June 23, 2017Publication date: November 2, 2017Inventor: Richard M. Cawthon
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Publication number: 20170314069Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.Type: ApplicationFiled: February 13, 2017Publication date: November 2, 2017Applicant: Rubicon Genomics, Inc.Inventors: Vladimir L. MAKAROV, Emmanuel KAMBEROV, Brendan J. TARRIER
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Publication number: 20170314070Abstract: The present invention provides facile and accurate molecular diagnosis of disease-specific genes capable of the naked eye detection through amplifying the target genes to selectively block the fluid path in a microfluidic device. Specifically, the present invention includes an isothermal amplification of target genes through a rolling circle amplification, a microfluidic device for detecting pathogen genes, and a detection method using the same. Therefore, the present invention can conveniently detect a single target gene, such as a single pathogen, or at the same time, several target genes, such as several pathogens, without complicated mechanical equipment.Type: ApplicationFiled: October 30, 2015Publication date: November 2, 2017Inventors: Hyukjin LEE, Haeshin LEE, Ho Yeon LEE, Il Young JUNG
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Publication number: 20170314071Abstract: Provided are compositions and processes that utilize genomic regions that are differentially methylated between a mother and her fetus to separate, isolate or enrich fetal nucleic acid from a maternal sample. The compositions and processes described herein are particularly useful for non-invasive prenatal diagnostics, including the detection of chromosomal aneuploidies.Type: ApplicationFiled: February 9, 2017Publication date: November 2, 2017Inventors: Mathias EHRICH, Anders Olof Herman NYGREN, Tyler Jacob JENSEN
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Publication number: 20170314072Abstract: Provided are sequencing-by-binding methods of detecting cognate nucleotides using a crippled DNA polymerizing enzyme that possesses the ability to bind the next correct nucleotide downstream of a primer in a template-dependent fashion, but does not possess the activity needed to promote phosphodiester bond formation. Use of the crippled DNA polymerase permits interrogation of one nucleotide at a time, without incorporation of any nucleotide. Labeled nucleotides, such as fluorescently labeled nucleotides, can be used in conjunction with the crippled DNA polymerase to establish cognate nucleotide identity in a rapid manner.Type: ApplicationFiled: April 28, 2017Publication date: November 2, 2017Applicant: Omniome, Inc.Inventors: Kandaswamy Vijayan, Pinar Iyidogan
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Publication number: 20170314073Abstract: The present invention relates to methods to detect an amount of DNA that originates from cells of a given type, where the sample comprising such DNA in admixture with DNA that does not originate from such cells. Such methods are based on differential methylation, at certain regions, of the DNA that originates from the given type of cells compared to the admixed DNA. Such methods have particular application in the detection, from a biological fluid from a pregnant female, of cell free DNA that originates from a foetus or the placenta of a foetus, or the detection, from a biological fluid from an individual, of cell free DNA that originates from cells of a tumour.Type: ApplicationFiled: May 8, 2015Publication date: November 2, 2017Inventors: Sebastian Grömminger, Wera Hofmann, Hamed Said, Matthias Sachse
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Publication number: 20170314074Abstract: Provided herein are, inter alia, methods and biomarkers to determine if a patient has or is at risk for developing an LPA-associated disease, to indicate the progressiveness of the disease, and/or to facilitate evaluation of responsiveness to therapy. Compositions and kits including the biomarker proteins and biomarker RNAs provided herein are further provided.Type: ApplicationFiled: November 15, 2016Publication date: November 2, 2017Inventors: Karl Kossen, Sharlene R. Lim, Scott D. Seiwert, Donald Ruhrmund
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Publication number: 20170314075Abstract: The presently disclosed subject matter is directed to methods of aiding diagnosis, prognosis, monitoring and evaluation of a disease or other medical condition in a subject by detecting a biomarker in microvesicles isolated from a biological sample from the subject.Type: ApplicationFiled: May 5, 2017Publication date: November 2, 2017Applicant: THE GENERAL HOSPITAL CORPORATIONInventors: Johan Karl Olov Skog, Xandra O. Breakefield, Dennis Brown, Kevin C. Miranda, Leileata M. Russo
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Publication number: 20170314076Abstract: The present invention relates to systems, devices and methods for diagnosing cancer. In various embodiments, the present invention provides a method for quantifying a 5?-htRNA; a method for quantifying a 3?-htRNA; a method for obtaining a DNA library of 5?-htRNAs and a DNA library of 5?-htRNAs obtained therefrom; and a method for obtaining a DNA library of 3?-htRNAs and a DNA library of 3?-htRNAs obtained therefrom. The invention also teaches a method for determining the presence or absence of a cancer cell in a biological sample; a method of diagnosing cancer in a subject; and a method of prognosing cancer in a subject.Type: ApplicationFiled: February 4, 2015Publication date: November 2, 2017Applicant: THOMAS JEFFERSON UNIVERSITYInventors: Yohei Kirino, Shozo Honda
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Publication number: 20170314077Abstract: The use of Slfn11 as a biomarker for detecting the occurrence of epithlial-to-mesenchymal transition (EMT) in a subject, and the use of Slfn11 modulators to treat cancer is disclosed herein. Also disclosed are various methods for detecting the occurrence of epithelial-to-mesenchymal transition (EMT) in a subject by measuring Slfn11 expression and/or activity.Type: ApplicationFiled: November 13, 2015Publication date: November 2, 2017Applicant: BERGENBIO ASInventors: David Robert MICKLEM, Monica HELLESOY, Linn Hodneland NILSSON
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Publication number: 20170314078Abstract: The present technology relates to genetic products the expression of which is associated with cancer diseases. The present technology also relates to the therapy and diagnosis of diseases in which the genetic products are expressed or aberrantly expressed, in particular cancer diseases.Type: ApplicationFiled: May 10, 2017Publication date: November 2, 2017Inventors: Ugur Sahin, Özlem Türeci, Michael Koslowski
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Publication number: 20170314079Abstract: Anti-EGFR antibodies, therapeutic compositions comprising combinations of anti-EGFR antibodies, as well as methods for using such antibodies and compositions to treat EGFR-related disorders (e.g., cancers), are disclosed.Type: ApplicationFiled: June 22, 2017Publication date: November 2, 2017Inventors: Raghida BUKHALID, Michael J. FELDHAUS, Anne KING, Neeraj KOHLI, Eric M. KRAULAND, Jeffrey D. KEARNS, Alexey Alexandrovich LUGOVSKOY, Ulrik B. NIELSEN
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Publication number: 20170314080Abstract: Robust and reliable molecular diagnostic screening tools for early detection of esophageal and gastrointestinal tract cancers and pre-cancerous lesions, such as Barrett's Esophagus, and esophageal adenocarcinoma are provided. Included in the invention is an array of miRNA probes specific for identifying, diagnosing and prognosticating esophageal and gastrointestinal tract cancers and pre-cancerous lesions in subjects from blood or serum samples. A biochip comprising the array as well as methods for its use are also provided.Type: ApplicationFiled: July 12, 2017Publication date: November 2, 2017Inventors: Stephen J. Meltzer, Yulan Cheng, Jee-Hoon Song