Abstract: A system and method of predicting impending failure of a pressure vessel include a pressure vessel, a fluid source, a line coupled to the pressure vessel and to the fluid source, an apparatus, a sensor and a controller. The apparatus includes a conduit and a containment structure. The containment structure includes a cavity separated from an interior of the conduit by a portion of a conduit wall of the conduit. The sensor is configured to determine a value of a physical property in the cavity. The controller is in signal communication with the sensor and configured to detect a change in the value. The method includes determining a first value of a physical property in the cavity, experiencing a failure of the conduit wall, determining a second value of the physical property in the cavity, and detecting a difference between the first and second values.

Abstract: An acoustic wave detector may include: an exterior housing with an exterior housing wall, a gas chamber located within the exterior housing and configured to receive a gas therein. The exterior housing wall may include an aperture providing a gas passage between the gas chamber and the exterior of the acoustic wave detector. The acoustic wave detector may further include an excitation element configured to selectively excite gas molecules of a specific type in the gas received in the gas chamber in a time-varying fashion, thereby generating acoustic waves in the gas, and an acoustic wave sensor configured to detect the acoustic waves generated in the gas and acoustic waves generated outside of the acoustic wave detector. The acoustic wave sensor may have an acoustic port overlapping with the aperture in the exterior housing wall.

Abstract: With a detector in which detection elements are placed in a spherical shape, a uniform resolution area is narrow. An acoustic-wave acquisition apparatus of the present invention is equipped with a detector including a plurality of detection elements that receive acoustic waves from a subject, the receiving surfaces of at least some of the detection elements being at different angles. The apparatus includes a scanning unit configured to move at least one of the subject and the detector to change the relative position of the subject and a highest-resolution area determined depending on the placement of the detection elements.

Abstract: A method for detecting for the presence of H2S or HS? anion in a system, comprising contacting a sample from the system with a compound, or a protonate or salt thereof, having a structure represented by: wherein Y represents an aromatic group or a substituted aromatic group; n is 1 or 2; R is independently H, alkyl, substituted alkyl, a polyether moiety, carboxyl, substituted carboxyl, carbamate, substituted carbonate, carbonyloxy, alkoxy, substituted alkoxy, haloalkyl, halogen, nitro, amino, amido, aryloxy, cyano, hydroxyl, or sulfonyl; R1 is H, substituted lower alkyl, lower alkyl, substituted aralkyl or aralkyl; R2 is selected from H, acyl, substituted aralkyl, aralkyl, phosphonyl, —SO2R3; —C(O)R5; —C(O)OR7 or —C(O)NR9R10; R3; R5; R7; R9 and R10 are each independently selected from H, substituted lower alkyl, lower alkyl, substituted aralkyl, aralkyl, substituted aryl or aryl.

Abstract: Disclosed is a gas sensing material for methylbenzene detection. Specifically, the gas sensing material includes a nanocomposite of Cr2O3 and ZnCr2O4. The content of Cr in the nanocomposite is from 67.0 at. % to 90.0 at. %, based on the sum of the contents of Cr and Zn atoms. The gas sensing material is highly selective to methylbenzenes over other gases and is highly sensitive to methylbenzenes. Also disclosed are methods for preparing the gas sensing material. The methods facilitate control over the composition of the gas sensing material and enable rapid synthesis of the gas sensing material at low temperature. Also disclosed is a gas sensor including the gas sensing material.

Abstract: Provided are: a system for predicting the residual lifespan of a fire-damaged concrete structure and a method therefor, the system being capable of acquiring sample data by performing a chemical analysis on a sample obtained from the fire-damaged concrete structure, comparing the obtained sample data with data pre-stored in a standardization DB so as to quickly predict the residual lifespan of the fire-damaged concrete structure, and accurately and scientifically assessing the level of fire damage of the fire-damaged concrete structure so as to perform appropriate repair and reinforcement of the fire-damaged concrete structure.

Abstract: A multi-frequency inductive sensing system can be used for spectrographic material analysis of a conductive target material (such as tissue) based on electrical impedance spectroscopy. An inductive senor can be driven with an excitation current at multiple sensor excitation frequencies (?) to project a time-varying magnetic field into a sensing area on the surface of the target material, inducing eddy currents within the target material. The inductive sensor can be characterized by a sensor impedance Z(?) as a function of the sensor excitation frequency (?), and the resulting induced eddy currents. Multiple sensor impedance Zs(?) measurements, at the multiple sensor excitation frequencies (?), can be determined, which represent electromagnetic properties of the target material (such as permittivity ?, permeability ?, and resistivity ?), based on the induced eddy currents.

Abstract: An imaging system for imaging live biological systems comprises a detector array (12a) having an optical axis (X-X) and arranged to detect light and output detector signals, a support (10) arranged so support a biological system on the optical axis, an illuminating light source (16) located off the optical axis and arranged to direct at least partially-coherent light towards the biological system, and processing means (18) arranged to receive the detector signals and generate image data.

Abstract: A method for determination of the growth rate of biofilm (7) using an electrical impedance analyses is disclosed. The method comprises the steps of: bringing a culture medium fluid (3) in contact to an electrode structure (4a, 4b), having biofilm (7) grown within the fluid culture medium (3) with the biofilm (7) arranged in distance to the electrodes structure (4a, 4b), so that the fluid culture medium (3) is placed between the growing biofilm (7) and the electrode structure (4a, 4b); measuring the impedance of the electrodes structure (4a, 4b) over a monitoring time, and determining the growth rate of the biofilm (7) as a function of the reduction rate of the impedance values measured on the electrode structure (4a, 4b).

Abstract: A biosensor for single cell analysis is disclosed. The biosensor includes a substrate, an array of electrodes, and a passivation layer. The substrate includes a roughened surface, where the array of electrodes is patterned on the roughened surface. Each electrode includes a distal tip and a proximal end. The passivation layer is deposited on top of the biosensor and includes a microwell around the distal tip of an electrode. A single cell is trapped within the microwell and adhered onto the distal tip of the electrode for further single cell analysis.

Abstract: A system for monitoring the sobriety of a user is provided. The system may include a testing device that generates a substance content signal. The testing device may further include a mouthpiece and a user identification device. The user identification device may generate user identification data in response to a user's breath and may transmit it from the testing device to a monitoring station. The testing device may further include at least one of an LCD screen or a light-emitting diode (“LED”) light. At least one of the LCD screen or the LED light may display at least one randomly generated visible identification indicia.

Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.

Abstract: Hoxb5 identifies long-term hematopoietic stem cells. Expression of Hoxb5 distinguishes between LT-HSCs and non-LT-HSCs, and the marker identifies substantially all LT-HSC in the bone marrow. By utilizing fluorescent proteins under the endogenous expression control of Hoxb5, LT-HSC can be monitored and isolated, including without limitation detection and monitoring of HSC in bone morrow; production of LT-HSC from pluripotent stem cells such as iPS cells; for analysis of early stage LT-HSC; in screening methods for expansion and manipulation of LT-HSC, and the like.

Abstract: An in-vitro diagnostic includes a housing, a storage, and a blocking agent. The housing houses a liquid including a test substance included in a sample extracted from a subject. The storage stores a substance that specifically reacts with the test substance. The blocking agent is placed to separate the container and the storage.

Abstract: The present invention relates to a composition for detecting phosphatase or kinase activity and a method of detecting phosphatase or kinase activity. The kinase or phosphatase activity may be quantitatively measured in real time by using the composition of the present invention.

Abstract: A microdevice for monitoring a target analyte is provided. The microdevice can include a field effect transistor comprising a substrate, a gate electrode, and a microfluidic channel including graphene. The microfluidic channel can be formed between drain electrodes and source electrodes on the substrate. The microdevice can also include at least one aptamer functionalized on a surface of the graphene. The at least one aptamer can be adapted for binding to the target analyte. Binding of the target analyte to the at least one aptamer can alter the conductance of the graphene.

Abstract: Methods are disclosed for tethering a biological entity to a substrate comprising: (a) forming a supported lipid bilayer on a surface of a substrate, wherein the supported lipid bilayer comprises an anchor molecule conjugated to a first affinity tag that is present in the lipid bilayer at a concentration greater than or equal to 5 mole percent; and (b) contacting the supported lipid bilayer with a biological entity, wherein the biological entity comprises an nonlinear-active label and a second affinity tag capable of binding to the first affinity tag, thereby tethering the biological entity to the supported lipid bilayer in an oriented fashion.

Abstract: The present invention relates to a biomarker for diagnosing rheumatoid arthritis, a biomarker for diagnosing osteoporosis as a complication of rheumatoid arthritis, a biomarker for predicting the severity and prognosis of rheumatoid arthritis, and a use thereof.

Abstract: The disclosure provides methods, compositions, and kits for enhanced detection of microbes in samples and monitoring of antimicrobial activity in a subject.

Abstract: The invention relates to an expression construct for the soluble expression of polypeptides in host cells and for the expression of polypeptides on the surface of host cells, using alternative splicing. The amount of cell membrane expression of polypeptides such as antibodies, antibody fragments and bispecific antibodies can be correlated directly to the amount of soluble polypeptide expressed. In the case of bispecific antibodies, cell membrane expression of heterodimer and homodimer products can be correlated directly to the soluble expression of these products, thereby aiding selection of a desired producer clone.

Abstract: This invention also relates to recombinant vectors expressing one or more of the human CMV (HCMV) glycoproteins US2, US3, US6 and US11 or corresponding functional rhesus CMV (RhCMV) homologues Rh182, Rh184, Rh185 or Rh189, methods of making them, uses for them, expression products from them, and uses for the expression products. This invention also relates to recombinant cytomegalovirus vectors vectors lacking one or more of the glycoproteins, methods of making them, uses for them, expression products from them, and uses for the expression products.

Abstract: The present invention provides self-contained systems for performing an assay for determining a chemical state, the system including a stationary cartridge for performing the assay therein, at least one reagent adapted to react with a sample; and at least one reporter functionality adapted to report a reaction of the at least one reagent with said sample to report a result of the assay, wherein the at least one reagent, the sample and the at least one reporter functionality are contained within the cartridge.

Abstract: The present invention relates to the use of a nucleic acid molecule encoding a first reporter gene, bordered by at least one first pair and one second pair of sequences targeting a site-specific recombinase in order to detect cells of a mammal infected with a virus responsible for an immunodeficiency.

Abstract: The present disclosure provides methods, reagents, systems, and devices that target ? lactamase as a biomarker for the sensitive and specific detection of tuberculosis-complex bacteria. Specifically, the present disclosure relates to methods and compositions for the detection of specific ?-lactamase protein and nucleic acid sequences to indicate the presence of tuberculosis-complex bacteria.

Abstract: Light-generating fusion proteins having a ligand binding site and a light-generating polypeptide moiety and their use as diagnostics, in drug screening and discovery, and as therapeutics, are disclosed. The light-generating fusion protein has a feature where the bioluminescence of the polypeptide moiety changes upon binding of a ligand at the ligand binding site. The ligand may be, for example, an enzyme present in an environment only under certain conditions, e.g., ubiquitin ligase in a hypoxic state, such that the light-generating fusion protein is “turned on” only under such conditions.

Abstract: A monoclonal antibody to KIR2DS1 or a fragment containing an antigen-binding region thereof has VL having an amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having the same amino acid sequence as the amino acid sequence except that one or several amino acids are conservatively substituted as CDR1, having an amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having the same amino acid sequence as the amino acid sequence except that one or several amino acids are conservatively substituted as CDR2, and having an amino acid sequence of SEQ ID NO: 3 or an amino acid sequence having the same amino acid sequence as the amino acid sequence except that one or several amino acids are conservatively substituted as CDR3; and VH having an amino acid sequence of SEQ ID NO: 4 or SEQ ID NO: 5 or an amino acid sequence having the same amino acid sequence as the amino acid sequence except that one or several amino acids are conservatively substituted as CDR1, having an amino acid sequence of SEQ ID NO

Abstract: The invention relates to a method of determining an increased risk of cancer in an immunosuppressed patient, the method comprising: (a) determining the percentage of CD8+CD57+ T-cells in a population of CD8+ T-cells in a sample from the patient; wherein a percentage of 40% or greater of CD8+CD57+ T-cells is indicative of an increased risk of cancer; and/or (b) determining the percentage of CD4+CD57+ T-cells in a population of CD4+ T-cells in a sample from the patient; wherein a percentage of 10% or greater of CD4+CD57+ T-cells is indicative of an increased risk of cancer.

Abstract: The present invention provides a method for the diagnosing and monitoring of bladder cancer.

Abstract: The present technology generally relates to methods and compositions relevant to the prediction that a subject with and/or after treatment for DCIS will experience a subsequent ipsilateral breast event that is a DCIS recurrence, an invasive breast cancer, both a DCIS recurrence and invasive cancer, or neither. The technology can assist one with how to treat such subjects.

Abstract: Provided herein are assays and methods related to determining a ratio of expression levels of PSA/PSMA or determining the expression level of PSMA in circulating tumor cells for diagnosis and/or for the purpose of monitoring treatment efficacy for prostate cancers that are likely hormone resistant.

Abstract: Methods and compositions for diagnosing and treating diseases, particularly cancer, associated with differential expression of cancer-associated targets (CAT) in disease cells compared to healthy cells are provided. Also provided are antagonists and agonists of CAT, and methods for screening agents that modulate CAT level or activity in vivo or in vitro.

Abstract: Screening human subjects for vector-borne disease is provided and carried out using thymidine kinase 1 alone or in combination with c-reactive protein as biomarkers which are measured in a blood sample, and the measurements are used to compute an index that allows a practitioner to compare results from different subjects and between different populations of subjects, and the screening for vector-borne disease can also be used on subjects that are undergoing treatment to monitor the progress of an infection and the results of the treatment.

Abstract: A diabetes management system includes a handheld medical device, a mobile computing device, and a diabetes management application. The handheld medical device is configured to determine, in response to a port receiving a test strip, whether an auto-send feature is enabled on the handheld medical device, determine whether the handheld medical device is paired with a mobile computing device, and selectively instruct a wireless transceiver to establish a wireless connection and communicate a glucose measurement and identifying information to the mobile computing device. The mobile computing device is configured to execute the diabetes management application. The diabetes management application is configured to process a plurality of glucose measurements and identifying information associated with each of a plurality of glucose measurements.

Abstract: Detecting glycosaminoglycans (GAGs) and/or determining the level of one or more glycosaminoglycans can be useful, e.g., in identifying or monitoring various medical conditions, the status of patients having various medical conditions, and/or the response to treatment of individuals having various medical conditions. The present invention provides methods for detecting glycosaminoglycans and/or determining the level of glycosaminoglycans through the use of, e.g., mass spectrometry.

Abstract: Provided herein is synthesis of a novel acidic acid residue targeting sulfoxide-containing MS-cleavable homobifunctional cross-linker. The novel mass spectrometry-cleavable cross-linking agents can be used in mass spectrometry to facilitate structural analysis of intra-protein interactions in proteins and inter-protein interactions in protein complexes. Also disclosed herein are data based on the novel MS-cleavable homobifunctional cross-linker that are complimentary to amine-reactive sulfoxide-containing MS-cleavable reagents.

Abstract: The present disclosure relates to a method for validating an existence of a urinary exosome including steps as follows. A urine sample is obtained from a subject. The urine sample is performing a serially centrifugation step to obtain a third precipitate. The third precipitate is resuspended with an extraction solvent to obtain a third mixture, and the third mixture is centrifuged to obtain a fourth supernatant. The fourth supernatant is analyzed by a mass spectrometry to detect whether there is a particular peptide therein.

Abstract: The methods described herein enable the evaluation of compounds on subjects to assess their therapeutic efficacy or toxic effects. The target of analysis is the underlying biochemical process or processes (i.e., metabolic process) thought to be involved in disease pathogenesis. Molecular flux rates within the one or more biochemical processes serve as biomarkers and are quantitated and compared with the molecular flux rates (i.e., biomarker) from control subjects (i.e., subjects not exposed to the compounds). Any change in the biomarker in the subject relative to the biomarker in the control subject provides the necessary information to evaluate therapeutic efficacy of an administered drug or a toxic effect and to develop the compound further if desired. In one aspect of the invention, stable isotope-labeled substrate molecules are administered to a subject and the label is incorporated into targeted molecules in a manner that reveals molecular flux rates through one or more metabolic pathways of interest.

Abstract: A test apparatus and method for measuring a concentration of a target by correcting for an impact of hemoglobin are provided. The target measurement method includes measuring an absorbance of hemoglobin in a sample, measuring an absorbance of a target in the sample, determining variation of the absorbance of the target according to the measured absorbance of the hemoglobin, and correcting the absorbance of the target by subtracting the determined variation of the absorbance of the target from the measured absorbance of the target.

Abstract: The present invention relates to a container comprising haemoglobin fractions, wherein said container comprising at least two compartments, wherein a first compartment comprises O2Hb (oxyhaemoglobin) and a second compartment comprises MetHb (methaemoglobin), optionally wherein O2Hb is stabilized. The invention also relates to a kit for determining the reliability of a CO-oximetry device, wherein said kit comprises said container and to a method for determining the reliability of a CO-oximetry device using said container.

Abstract: The present invention relates to device (10) for determining a fibrinogen level (20) in a sample (22) comprising, a first input for obtaining an attenuance signal (24) over time indicative of a fibrin polymerization of said sample (22), a second input for obtaining a reactant concentration signal (28) over time indicative of a reactant concentration in said sample (22), wherein the reactant is any substance leading to the cleavage of fibrinogen to fibrin, a simulation unit (16) running a model (32) using the reactant concentration signal (28) as an input to provide a simulated attenuance signal (34) over time, and an evaluation unit (18) configured to infer the fibrinogen level (20) of said sample (22) by comparing the attenuance signal (24) over time with the simulated attenuance signal (34) over time.

Abstract: The present invention relates to a method for acquiring information on a cause of prolongation of coagulation time. The present invention also relates to a device, a system and a computer program for analyzing blood coagulation.

Abstract: Provided herein are methods for determining the ratio of one or more isoforms of a protein in a sample.

Abstract: A cell smearing apparatus comprising: a mixed solution container accommodation unit accommodating a mixed solution container containing a mixed solution of cells and a preservation solution; a suction unit suctioning the mixed solution from the mixed solution container accommodated in the mixed solution container accommodation unit; a sensor unit sensing variation of the mixed solution suctioned by the suction unit; and a controller controlling a suctioning speed of the suction unit based on a value sensed by the sensor unit. The present invention allows cells to be examined to be smeared as a monolayer on a slide for microscopic examination.

Abstract: A substrate for sample analysis including: a substrate including a rotation axis; a first chamber, which includes a first space which retains the liquid; a second chamber, which includes a second space which retains the liquid discharged from the first chamber; and a first flow passage, which includes a path connecting the first chamber and the second chamber in which the first flow passage has a first opening and a second opening, the first opening and the second opening are connected to the first chamber and the second chamber, respectively, and the first opening is positioned on a side closer to the rotation axis than the second opening, in which the first space includes a first region, which includes a portion extending from the first opening and in which the first space of the first chamber has a capacity larger than a capacity of the first flow passage.

Abstract: The invention provides systems, compositions, kits and methods for automated processing of biological samples and analysis using a flow cytometer.

Abstract: A method for supplying consumable items to an automated sample analyzer is presented. The automated analyzer comprises a consumable repository including two or more storage areas. Each area is configured to store a plurality of consumable items. The method comprises receiving one or more orders for analytical tests to be carried out by the analyzer, reserving consumable items corresponding to one or more sets of consumable items required for the analytical tests in a first area, switching to a second area for reserving consumable items in response to determining that a predetermined consumable item in the first area has been reserved, supplying the reserved consumable items for use in the one or more analytical tests and indicating that the first area can be replenished in response to determining that a particular consumable item of the first storage area has been used.

Abstract: A suspension system for a health and usage monitoring system including an inertial measurement unit is provided that includes a plurality of elastomer standoffs coupled between an enclosure of the health and usage monitoring system and an electronics board of the health and usage monitoring system thereby allowing for hysteretic damping to reduce vibration and shock experienced by the electronics board and inertial measurement unit.

Abstract: A system for predicting exterior ballistics has first and second bullet detectors operable to detect the passage of a bullet, the first and second bullet detectors being spaced apart by a selected detector spacing distance, the first and second bullet detector each being connected to a common time signal facility that generates a time signal, the first bullet detector being operable to generate a first time of passage based on the time signal, the second bullet detector being operable to generate a second time of passage based on the time signal, the first bullet detector being operable to measure a first bullet velocity, a controller in communication with the first and second bullet detectors, and the controller operable based on the difference between the first time and the second time, and based on the first bullet velocity to calculate a ballistic characteristic for the bullet.

Abstract: Apparatuses, systems, and methods are provided for measuring the velocity and direction of a fluid flow. In some instances, a measuring system may include a housing capable of holding one or more pressure sensors in a desired location and orientation. The housing may include a cavity for each of the one or more pressure sensors and each cavity may have a connection to an opening at the outside surface of housing. Each opening may be able to face in any desired direction such that the pressure at any desired location on the outside surface of housing, which may be capable of facing in any desired direction, may extend to the cavity inside housing where it can be measured by a pressure sensor. The velocity and the direction of a fluid flow around the housing of the measurement system may be based on pressure readings generated by the pressure sensors.

Abstract: A vibration sensor having a moveable mass being suspended in a suspension member and being adapted to move in response to vibrations or accelerations. The moveable mass and the suspension member are rigidly connected across one or more gaps formed by respective opposing surfaces of the moveable mass and the suspension member. The vibration sensor includes a damping arrangement having a damping substance. The moveable mass is arranged to interact directly or indirectly with the damping substance in order to reduce a mechanical resonance peak of the vibration sensor.