Abstract: A viscous fluid cleaning composition for a cleaning a substrate comprising (a) a surfactant combination comprising (i) at least one surfactant; and (ii) a glycolipid biosurfactant which is present at a level in the range 10-95 wt % of the total surfactant in said surfactant system, and (b) one or more viscosity modifiers; and (c) ethoxylated polyethylene imine (EPEI) and (d) a volatile benefit agent; wherein the composition has a pour viscosity as measured at 21 s-1 of between 250 CPs to 3000 Cps.

Abstract: Stabilized hydrogen peroxide-containing compositions and methods of making same are disclosed. The compositions contain a stabilizer system made up of a disulfonate surfactant, a diester solvent, and a sulfonic acid or a salt thereof in a sufficient quantity to provide the stabilized hydrogen peroxide with an acidic pH value. The compositions are suitable for use as disinfectants, as cleaning agents, and in various personal care applications such as hair care and tooth whitening.

Abstract: Provided herein are solid compositions for fabric treatment; aerated solid compositions for fabric treatment; unit dose laundry detergent compositions containing solid compositions or aerated solid compositions for fabric treatment; and methods of their making and using, e.g., for treating textiles.

Abstract: Method and apparatus for creating lenticular, or other types of images that change with respect to the angle from which images are viewed in a bar of soap, including a method of printing a lenticular, “fly's-eye” or other type of image onto either the soap itself, or on a non-toxic, food-safe, substrate with non-toxic, food-safe inks, or by projecting an image onto non-toxic, food-safe photo-sensitive emulsion layer within the soap, and creating a lenticular, or other type of lens array with a clear or sufficiently transparent soap material as to display lenticular or other integrated image properties, such as 3D, motion, flip, zoom, color change, morph, and others. Additionally, the lenticular array is located within the soap bar such that the soap will not destroy the image when first used, but only after a large portion or almost all of the soap is used up or consumed.

Abstract: A composition and method of treatment for handling potential prion contamination of surfaces is disclosed.

Abstract: A method of preparing a wort with an increased level of free amino nitrogen (FAN) comprising: a) preparing a mash from a grist comprising malt and/or adjunct; and b) adding a protease having at least 80% sequence identity to the polypeptide of SEQ ID NO: 1.

Abstract: Processes and systems for recovering products from a corn fermentation mash. In one example, a process for recovering products from a corn fermentation mash can include separating ethanol from a fermentation mash to produce a whole stillage. The fermentation mash can be derived from a ground corn product milled from a plurality of corn pieces. The plurality of corn pieces can include whole corn kernels, fragmented corn kernels, size-reduced corn kernels, milled corn kernels, or a mixture thereof. Greater than 25 wt % of the ground corn product can have a particle size of greater than 105 ?m and greater than 80 wt % of the ground corn product can have a particle size of 425 ?m or less, as measured according to AOAC 965.22-1966. The process can also include separating the whole stillage to produce a fiber rich portion and a filtrate.

Abstract: An infuser for alcoholic beverages, having a container of ethyl alcohol and a water source, both of which are hydraulically connected to a volumetric mixing chamber, a water capsule holder located downstream of the volumetric chamber and intended to receive capsules containing single doses of essences, and extracting liquid from the volumetric chamber and injecting same into a capsule. The ethyl alcohol container and the water source are connected to the volumetric chamber by shut-off solenoid valves, and the volumetric chamber contains a main electrode and a plurality of secondary electrodes disposed above the main electrode at different levels.

Abstract: The present invention provides an immobilized reaction device and a method for carrying out reaction by utilizing the immobilization technology. The immobilized reaction device includes a columnar reactor with an inlet and an outlet. The reactor is provided with an interior cavity which is defined by a top portion and a bottom portion opposite to each other, and a side wall connecting the top portion and the bottom portion.

Abstract: The presently disclosed subject matter provides a microfluidic device that can simulate capillary blood flow on a fetal side of the device and pooled blood on a maternal side of the device (i.e., intervillous space). The microfluidic device can reconstitute the maternal-fetal interface, can expand the capabilities of cell culture models, and can provide an alternative to current maternal-fetal transfer models.

Abstract: A cell culture device includes: an incubator part for accommodating a closed-system culture container; a cartridge for culture medium replacement use having a liquid supply flow path and a liquid collection flow path, the cartridge removably attachable to the culture container; a culture medium supply part for supplying a liquid culture medium to the liquid supply flow path; a culture medium replacement part for causing the liquid culture medium in the liquid supply flow path to flow into the culture container and causing the liquid culture medium in the culture container to flow out to the liquid collection flow path while the cartridge is connected to the culture container; and a culture medium collection part for collecting the liquid culture medium from the liquid collection flow path. The cartridge is movable between the culture medium supply part, the culture medium replacement part and the culture medium collection part.

Abstract: The present invention provides an incubator with a clean bench function to have both function of opened and closed system and a proposal how to use, allowing cells or others to be “closable and openable” manipulated and cultured, as well as an incubator, a globe box, a clean bench. This is implemented in this system has a specific structure and a characteristic component, e.g., a structure classified two units by the inside temperature, an operation cover and a circular fixture, a channel from/to inside or outside etc. to allow such as used, a non-opening operation and supply and observation from outside, or a moving of perfusion culture device and medium storing bags kept on connecting tubes or others. An incubator 100 with a clean bench function mainly includes a chamber 110, 210, 220, 230, a temperature control unit 160, 213 and a gas concentration control and supply unit 140, 240, as well as a CO2 cylinder 170, a N2 cylinder 180, and a N2 gas generator 190.

Abstract: The invention relates to yeasts comprising a protection coating made of one or more compounds which are inert relative to said yeasts, characterized in that the yeasts are dried active yeasts. Application in the preparation of food products for animals.

Abstract: The disclosure provides methods and compositions to improve the nutritional conditions, such as reducing the use of fertilizers applied during the growing season, and tolerance to fungal pathogens in tomato and potato plants.

Abstract: The invention provides a novel method for controlling and/or treating the growth of biofilms by promoting growth of the periphery cells and/or increasing nutrient consumption by the periphery cells so that the growth of peripheral cells is not only independent from nutrients (e.g., ammonium) provided by the interior cells of the biofilms, but also causes interior cells starved to death. The accessible peripheral cells are then treated and/or eliminated by biofilm control substance of antibiotics or toxic chemicals. Therefore, the invention method controls and eliminates both peripheral and interior cells within biofilms. The invention method can be used in various industries, such as clinical and dental medicine and medical equipment, food and oil industry, and water supply systems.

Abstract: The invention relates to biologically functional scaffolds having a porous structure, methods of preparing, and methods of use thereof. The invention also relates to methods of repairing a defect, methods of culturing cells and promoting differentiation of stem cells using the same.

Abstract: The present invention relates to a method for ex vivo photodynamic treatment of cells. A cell preparation is incubated in a first medium having 5 ?M or less of a photosensitive compound. A population of cells in the cell preparation retains the photosensitive compound, for example, a salt of 2-(4,5-dibromo-6-amino-3-imino-3H-xanthen-9-yl)-benzoic acid methyl ester. The first medium is then replaced with an extrusion medium to remove the photosensitive compound. The cell preparation is then illuminated in the extrusion medium to selectively kill the population of cells retaining the photosensitive compound, thereby creating an illuminated cell preparation.

Abstract: A modified immunocyte: (1) which expresses an exogenous unmodified T cell receptor ?-chain and an exogenous T cell receptor ?-chain on the cell surface thereof; or (2) which contains a polynucleotide encoding a T cell receptor ?-chain and a polynucleotide encoding a T cell receptor ?-chain. Thus, a new tool whereby immunity can be appropriately induced in vivo is provided.

Abstract: The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

Abstract: The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

Abstract: The present invention provides a method for producing megakaryocytes that have an increased capacity to produce platelets, the method comprising a step of culturing, under conditions that cause the death of cells that do not express a gene that is specifically expressed by megakaryocytes, cells that have the capacity to differentiate into megakaryocytes.

Abstract: A population of isolated mammalian macrophages, the cell surface of which is chemically modified to comprise a molecule that comprises an imaging agent or a therapeutic agent or a molecule that binds to an imaging agent or a therapeutic agent, and methods of making and using the modified macrophages are provided.

Abstract: The present invention relates to the ex vivo differentiation of NK cells from CD34+ hematopoietic stem cells. Such NK cells and their progenitor cells can be used in therapies of a broad range of malignancies. In the present invention it is shown that IL-12 modulates ex vivo NK cell differentiation. Specific, we achieved significantly higher expression of KIR, CD16 and CD62L in the presence of IL-12 in the cell culture system. The induction of receptor expression by IL-12 occurred predominantly on an augmented population of CD33+NKG2A+ NK cells early during NK cell differentiation. These cells further show enhanced cytolytic activity against MHC class I positive AML targets. In line with the enhanced CD16 expression, IL-12 modulated ex vivo generated NK cells exhibit an improved antibody-dependent-cytotoxicity, using anti CD20 antibody on various B cell targets. Additional to the enhanced expression of CD62L, we show that this cell population consists of a specific chemokine receptor profile.

Abstract: Some embodiments of the invention include methods for growing stem cells comprising growing the stem cells in a cell medium. In certain embodiments, the cell medium has a pH of from about 6.6 to about 7.2. Other embodiments of the invention embodiments include methods for transplanting comprising (a) growing first stem cells in a cell medium having a pH of from about 6.6 to about 7.2, to provide second stem cells and (b) transplanting the second stem cells into a recipient. Other embodiments include the methods where the stem cells that are human stem cells, mouse stem cells, rat stem cells, primate stem cells, or mammalian stem cells. Still other embodiments include growing stem cells in a cell medium having a pH of from about 6.6 to about 7.2 (e.g., from about 6.8 to about 7.0 or about 6.9) that results in modulation of one or more cell properties (e.g., decreased number of cells in the S phase, decreased amount of ROS, etc.) when that property is compared to that of stems cells grown at a pH of about 7.4.

Abstract: A tissue treatment apparatus and method for treating bone tissue has a controller, an enclosure, a reagent supply system, a draining configuration, and a gas relief valve is controlled by the controller. A bioactive agent may be applied through the reagent supply system. Optionally provided is a gas evacuation assembly, a gas supply unit, a thermal unit for heating or cooling reagents or gases, a sonication unit, any or each operated by the controller. The method provides for programming controller to effect a treatment procedure. In an embodiment of the present application the treatment procedure effects demineralization of the bone material.

Abstract: Methods of decontaminating bone tissue and an apparatus or system for the same are provided. The methods can be multi-batch processes and include contacting the bone tissue having contaminants with carbon dioxide to decontaminate the bone tissue and to form carbon dioxide having contaminants. The contaminated carbon dioxide is collected and the contaminants are removed to obtain purified carbon dioxide which can be recycled to treat contaminated bone tissue. The contaminated carbon dioxide can be purified by bubbling it through water and/or an organic solvent followed by acid treatment, filtering and liquefying the carbon dioxide. Contaminants that can be removed from contaminated bone tissue, and in turn, from contaminated carbon dioxide include infectious organisms, bacteria, viruses, protozoa, parasites, fungi and mold or a mixture thereof.

Abstract: The present invention relates to a method for producing a contractile cellular construct, comprising the steps of: a) seeding pre-selected cells onto a mold, wherein the pre-selected cells comprise signal emitting agents; and b) culturing the pre-selected cells to produce the cellular construct. The said method is exemplified by mixing cardiomyocytes with fluorescent microbeads, seeding the suspension on a polydimethylsiloxane (PDMS) mold and culturing to form a contractile device comprising of cardiac muscles. The present invention also relates to a method for measuring the contractility of the cellular construct by measuring the displacement of the signal emitting agents, and a method for screening a method for screening one or more agents for modulating the contractility of a contractile cellular construct.

Abstract: The invention provides methods for differentiating stem cells along the pancreatic lineage as well as large scale culture methods. The present invention further provides pancreatic progenitor cells derived from stern cells to provide pancreatic cells to a subject.

Abstract: The invention generally features compositions comprising induced pluripotent stem cell progenitors (also termed reprogramming progenitor cells) and methods of isolating such cells. The invention also provides compositions comprising induced pluripotent stem cells (iPSCs) derived from such progenitor cells. Induced pluripotent stem cell progenitors generate iPSCs at high efficiency. In particular embodiments the invention is predicated upon increased expression of an estrogen related receptor and changes in the oxidative and glycolytic pathways.

Abstract: Provided herein are compositions and methods for vaccination and research applications. In particular, provided herein are non-neuroinvasive herpesviruses and alpha herpesviruses and uses thereof.

Abstract: Compositions, methods of use and methods of manufacture are provided for PIV5-based amplifying VLP (AVLP) that can deliver an expressible heterologous nucleotide sequence in target cells without producing progeny, and which demonstrate useful safety and therapeutic efficacy in multiple animal and human health applications, such as vaccination, gene therapy and cancer therapy.

Abstract: The present invention relates to a recombinant host comprising a recombinant nucleic acid sequence encoding a polypeptide having at least about: a. 85% identity to the amino acid sequence set forth in SEQ ID NO: 1; b. 85% identity to the amino acid sequence set forth in SEQ ID NO: 3; c. 85% identity to the amino acid sequence set forth in SEQ ID NO: 6; d. 85% identity to the amino acid sequence set forth in SEQ ID NO: 9; e. 85% identity to the amino acid sequence set forth in SEQ ID NO: 11; f. 85% identity to the amino acid sequence set forth in SEQ ID NO: 14; g. 85% identity to the amino acid sequence set forth in SEQ ID NO: 17; h. 85% identity to the amino acid sequence set forth in SEQ ID NO: 20; i. 85% identity to the amino acid sequence set forth in SEQ ID NO: 22; or j. 85% identity to the amino acid sequence set forth in SEQ ID NO: 25.

Abstract: The present invention relates to isolated polypeptides having glucuronyl esterase activity, catalytic domains and polynucleotides encoding the polypeptides or catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

Abstract: The present invention relates to polypeptide fragments comprising an amino-terminal fragment of the PA subunit of a viral RNA-dependent RNA polymerase possessing endonuclease activity, wherein said PA subunit is from Influenza A 2009 pandemic H1N1 virus or is a variant thereof. This invention also relates to (i) crystals of the polypeptide fragments which are suitable for structure determination of said polypeptide fragments using X-ray crystallography and (ii) computational methods using the structural coordinates of said polypeptide to screen for and design compounds that modulate, preferably inhibit the endonucleolytically active site within the polypeptide fragment. In addition, this invention relates to methods identifying compounds that bind to the PA polypeptide fragments possessing endonuclease activity and preferably inhibit said endonucleolytic activity, preferably in a high throughput setting.

Abstract: The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Abstract: To provide an improved ?-fructofuranosidase which is capable of efficiently producing kestose while inhibiting the production of nystose. This improved ?-fructofuranosidase comprises either: an amino acid sequence (a) obtained by introducing, into the amino acid sequence represented by SEQ ID NO: 2, an amino acid mutation i) in which the 85th glycine (G) from the N-terminal is substituted for a protein-constituting amino acid other than glycine (G), and/or an amino acid mutation ii) in which the 310th histidine (H) from the N-terminal is substituted for lysine (K), arginine (R), or tyrosine (Y); or an amino acid sequence (b) which exhibits ?-fructofuranosidase activity, and which comprises an amino acid sequence obtained by deleting, substituting, inserting, or adding one or more amino acids in (a) other than the amino acid into which the amino acid mutation has been introduced.

Abstract: The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: The invention relates to a method for obtaining a mutant oleaginous yeast strain capable of growing on cellobiose as carbon source, comprising overexpressing in said strain two ?-glucosidase enzymes further comprising a N-terminal signal peptide. The invention also relates to a mutant yeast strain obtained by said method.

Abstract: The present invention provides a ?-agarase, a composition containing the same and applications thereof. The present ?-agarase provides the field a novel alternative and is favorable for the industrial utilities of the hydrolysis products of agarose. Furthermore, the present agarase is particularly modified for heterologous production by prokaryotic expression systems, and thereby is favorable for commercial use.

Abstract: The present invention relates to engineered cyanuric acid hydrolase enzymes that are resistant to hypochlorite and compositions and devices comprising such enzymes. The present invention also relates to methods of using these enzymes, compositions and devices for the treatment of a liquid, such as water.

Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.

Abstract: Reusable microsomal biocatalytic systems (bioreactors) constructed on carbon nanostructure modified electrodes are provided. The bioreactors comprise stable, biologically active immobilized enzymes such as human cytochromes P 450 (CYPs) and their redox partner proteins, e.g. CYP-NADPH (reduced nicotinamide adenine dinucleotide phosphate) reductases (CPR), on the carbon nanostructure surface. The immobilized enzymes may be present in liver microsomes, such as human liver microsomes (HLM) or as bactosomes, S9 fractions, etc. The bioreactors are used, for example, for synthesizing metabolites of interest from compounds such as drugs that are catabolized by the enzymes.

Abstract: The present invention relates to compositions and processes in the field of self-cleaning system using digestive proteins. One composition includes a substrate, a digestive protein capable of decomposing a stain molecule, and a linker moiety bound to both said digestive protein and said substrate. The processes include binding a substrate to a surface and forming a linker moiety between a digestive protein and said substrate.

Abstract: A method of DNA fragmentation is provided. The method comprising incubating a semi-solid biological sample which comprises the DNA with an auxiliary domain-directed nuclease having a binding affinity and selectivity to pre-defined sites in the DNA so as to yield a DNA fragment-of-interest, to thereby fragment the DNA.

Abstract: The present invention provides methods, compositions, and kits for generating rRNA-depleted samples and for isolating rRNA from samples. In particular, the present invention provides compositions comprising affinity-tagged antisense rRNA molecules corresponding to substantially all of at least one rRNA molecule (e.g., 28S, 26S, 25S, 18S, 5.8S and 5S eukaryotic cytoplasmic rRNA molecules, 12S and 16S eukaryotic mitochondrial rRNA molecules, and 23S, 16S and 5S prokaryotic rRNA molecules) and methods for using such compositions to generate rRNA-depleted samples or to isolate rRNA molecules from samples.

Abstract: Apparatuses for preparing a sample are disclosed herein. The apparatuses include a chamber, a first valve at least partially disposed in the first chamber, a second valve at least partially disposed in the first chamber, and a pump comprising an actuator and nozzle.

Abstract: The invention involves inducing a plurality e.g., 3-50 or more mutations (e.g., any whole number between 3 and 50 or more of mutations, with it noted that in some embodiments there can be up to 16 different RNA(s), e.g., sgRNAs each having its own a promoter, in a vector, such an AAV vector or a lentiviral vector and that when each sgRNA does not have its own promoter, there can be twice to thrice that amount of different RNA(s), e.g., sgRNAs, e.g., 32 or even 48 different guides delivered by one vector) in transgenic Cas9 eukaryotes to model a neuronal disease or disorder. The invention comprehends testing putative treatments with such models, e.g., testing putative chemical compounds that may be pharmaceutically relevant for treatment or gene therapy that may be relevant for treatment, or combinations thereof. The invention allows for the study of genetic diseases and putative treatments to better understand and alleviate a genetic disease or a condition, e.g.

Abstract: Provided herein are methods and systems for structure-assisted evolution of multivalent aptamers using a single stranded DNA or single-stranded RNA nanostructure as a structural support. Also provided herein are methods for constructing ssDNA or ssRNA nanostructures as structural supports suitable for structure-assisted evolution of multivalent aptamers.