Patents Issued in February 15, 2018
  • METHODS OF SCREENIING FOR MICROORGANISMS THAT IMPART BENEFICIAL PROPERTIES TO PLANTS
    Publication number: 20180044664
    Abstract: The present invention relates to methods for the screening, identification and/or application of microorganisms and/or compositions of use in imparting beneficial properties to plants, and microorganisms and compositions identified therefrom.
    Type: Application
    Filed: October 26, 2017
    Publication date: February 15, 2018
    Inventors: Peter John WIGLEY, Susan Jane Turner, Caroline Elizabeth George
  • METHOD OF MANUFACTURING PROTEIN ARRAY OR PEPTIDE ARRAY, METHOD OF IDENTIFYING FUNCTIONAL PROTEIN OR FUNCTIONAL PEPTIDE, PROTEIN ARRAY OR PEPTIDE ARRAY, AND FUNCTIONAL PROTEIN OR FUNCTIONAL PEPTIDE IDENTIFICATION KIT
    Publication number: 20180044665
    Abstract: There is provided a method of manufacturing a protein array or peptide array suitable for an efficient screening of a functional protein or functional peptide. The method of manufacturing a protein array or peptide array includes the steps of: (a) preparing a nucleic acid immobilized on a solid support and a cell-free synthesis system in a reactor, in which a reactor array includes the reactor having a specific aperture shape and a protein capture molecule or a peptide capture molecule provided on at least a portion of wall surface and bottom surface in the reactor; and (c) synthesizing a protein or peptide from the nucleic acid using the cell-free synthesis system and immobilizing the protein or peptide in the reactor.
    Type: Application
    Filed: October 13, 2017
    Publication date: February 15, 2018
    Applicants: THE UNIVERSITY OF TOKYO, NIKON CORPORATION
    Inventors: Takanori ICHIKI, Shingo UENO, Hisao OSAWA
  • GENOME-ORIGINATED ARTIFICIAL NCRNA EXPRESSION LIBRARY AND METHOD OF PREPARING THE SAME
    Publication number: 20180044666
    Abstract: The present invention relates to an artificial ncRNA expression library and a method for preparing the same, and particularly the present invention relates to a library securing the stability of the artificial ncRNA by cloning random fragmented DNA that whole genome DNA of E. coli is randomly fragmented to a middle of RNA scaffold having a double stem-loop and a method for preparing the same.
    Type: Application
    Filed: April 19, 2017
    Publication date: February 15, 2018
    Inventors: Younghoon LEE, Won Kyong KIM, Geunu BAK
  • Method for constructing nucleic acid single-stranded cyclic library and reagents thereof
    Publication number: 20180044667
    Abstract: Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagents thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5? end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.
    Type: Application
    Filed: October 14, 2014
    Publication date: February 15, 2018
    Inventors: Chunyu Geng, Rongrong Guo, Ruoying Chen, Lingyu He, Wenwei Zhang, Hui Jiang
  • MATE PAIR LIBRARY CONSTRUCTION
    Publication number: 20180044668
    Abstract: The present invention provides a novel method for ligating an adapter to a target polynucleotide and methods of generating a library of mate-pair polynucleotide constructs that employ such a ligation method. Libraries and arrays comprising mate-pair polynucleotide constructs, and methods of sequencing libraries and arrays comprising mate-pair polynucleotide constructs, are also provided.
    Type: Application
    Filed: October 13, 2015
    Publication date: February 15, 2018
    Applicant: BGI SHENZHEN CO., LIMITED
    Inventors: Yuan JIANG, Radoje DRMANAC, Evan HUROWITZ, Andrei ALEXEEV, Xia ZHAO, Jie RUAN
  • COMPOSITIONS FOR INHIBITING CHECKPOINT GENE EXPRESSION AND USES THEREOF
    Publication number: 20180044669
    Abstract: The present invention is directed to compounds, compositions, and methods useful for modulating PD1, PDL1, IDO1, LAG3, TIM3, CTLA4, IDO2, CEACAM1, OX40, and/or OX40L mRNA or protein expression using gene silencing compounds comprising two or more single stranded antisense oligonucleotides that are linked through their 5?-ends to allow the presence of two or more accessible 3?-ends.
    Type: Application
    Filed: October 26, 2017
    Publication date: February 15, 2018
    Inventors: Sudhir AGRAWAL, Wayne JIANG, Daqing Wang, Jessica Seitzer, Fu-Gang Zhu, Xianzhi Mao
  • GENE VECTOR
    Publication number: 20180044670
    Abstract: A gene vector for use in gene therapy comprising at least one miRNA sequence target operably linked to a nucleotide sequence having a corresponding miRNA in a hematopoietic progenitor cell (HSPC) or hematopoietic stem cell (HSC) which prevents or reduces expression of the nucleotide sequence in a HSPC or HSC but not in a differentiated cell.
    Type: Application
    Filed: August 31, 2017
    Publication date: February 15, 2018
    Applicant: Ospedale San Raffaele S.R.L.
    Inventors: Alessandra Biffi, Bernhard Rudolf Gentner, Luigi Naldini
  • DOUBLE-STRANDED OLIGONUCLEOTIDE MOLECULES TO P53 AND METHODS OF USE THEREOF
    Publication number: 20180044671
    Abstract: The present application relates to nucleic acid compounds, compositions comprising same and methods of use thereof for treatment of various diseases, disorders and conditions. The compounds are preferably chemically synthesized and modified double-stranded nucleic acid molecules which down regulate expression of a p53 gene.
    Type: Application
    Filed: September 12, 2013
    Publication date: February 15, 2018
    Applicant: QUARK PHARMACEUTICALS, INC.
    Inventors: Elena Feinstein, Sharon Avkin-Nachum, Hagar Kalinski, Igor Mett
  • Pericyte Long Non-Coding RNAs
    Publication number: 20180044672
    Abstract: The present invention provides novel non-coding RNAs (lncRNA) that were identified to be expressed in pericytes upon hypoxia. The lncRNA of the invention positively affect Platelet-derived Growth Factor Receptor (PDGFR) beta expression, pericytes proliferation and pericyte recruitment to endothelial cells. The invention provides inhibitors of the lncRNA for use in the treatment of diseases mediated by PDGFR expression. For example the invention described antisense approaches to target the lncRNA of the invention. Furthermore, the invention provides lncRNA inhibitors as amplifier of therapeutic PDGFR inhibitors such as imatinib or other tyrosine kinase inhibitors. lncRNA inhibitors and methods for screening modulators of lncRNA expression and/or function are provided.
    Type: Application
    Filed: March 18, 2016
    Publication date: February 15, 2018
    Inventors: Christoph Michael Zehendner, Stefanie Dimmeler, Andreas Zeiher
  • METHODS FOR MODULATING MECP2 EXPRESSION
    Publication number: 20180044673
    Abstract: Disclosed herein are methods for decreasing MECP2 mRNA and protein expression. Such methods are useful to treat, prevent, or ameliorate MECP2 associated disorders and syndromes. Such MECP2 associated disorders include MECP2 duplication syndrome.
    Type: Application
    Filed: March 3, 2016
    Publication date: February 15, 2018
    Applicants: Ionis Pharmaceuticals, Inc., Baylor College of Medicine
    Inventors: Huda Y. Zoghbi, Ezequiel Sztainberg, Susan M. Freier
  • NOVEL Zn-DPA COMPLEX COMPOUNDS AND siRNA DELIVERY SYSTEMS CONTAINING THE SAME
    Publication number: 20180044674
    Abstract: Provided herein are a novel Zn-DPA complex compound and an siRNA delivery system including the same as a transporter, the Zn-DPA complex compound including: a phosphate-directing functional part of zinc (II)-dipicolylamine (“Zn-DPA”); a cell membrane-directing functional part; and a linker part that links the phosphate-directing functional part and the cell membrane-directing functional part. The Zn-DPA complex compound has low toxicity and efficiently delivers siRNA to cells, thereby useful in various ways for various studies and diagnosis and treatment of diseases, which use siRNA.
    Type: Application
    Filed: August 8, 2017
    Publication date: February 15, 2018
    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Gyo Chang KEUM, Eun Kyoung BANG, Jin Bum KIM
  • ANTISENSE NUCLEIC ACIDS
    Publication number: 20180044675
    Abstract: Provided is a drug that allows highly-efficient skipping of exon. The present invention provides an antisense oligomer wherein two or more unit oligomers targeting sequences that are neither consecutive nor overlap with each other in the same exon are connected.
    Type: Application
    Filed: August 15, 2017
    Publication date: February 15, 2018
    Applicants: NIPPON SHINYAKU CO., LTD., NATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY
    Inventors: Naoki WATANABE, Yuuichirou TONE, Shin'ichi TAKEDA, Tetsuya NAGATA
  • COMPOSITIONS AND METHODS FOR MODULATING TTR EXPRESSION
    Publication number: 20180044676
    Abstract: Provided herein are oligomeric compounds with conjugate groups. In certain embodiments, the oligomeric compounds are conjugated to N-Acetylgalactosamine.
    Type: Application
    Filed: August 25, 2017
    Publication date: February 15, 2018
    Applicant: Ionis Pharmaceuticals, Inc.
    Inventors: Thazha P. Prakash, Punit P. Seth, Eric E. Swayze
  • Regulation of Translation of Expressed Genes
    Publication number: 20180044677
    Abstract: The present invention describes mRNA usage improving and/or translation-enhancing nucleic acid sequences, nucleic acid constructs comprising such sequences, and host cells comprising such nucleic acid constructs. The invention further pertains to a method for expressing a protein of interest in a cell or organismusing such nucleic acid sequences, as well as their uses for increasing integration of such nucleic acid construct into a genome, for enhancing mRNA usage and/or translation of a recombinantly expressed polypeptide, and for increasing the number of transformants upon transformation of a cell with such nucleic acid construct.
    Type: Application
    Filed: September 29, 2017
    Publication date: February 15, 2018
    Inventors: Raymond Michael Dimphena Verhaert, Pieter Victor Schut, Sharief Barends, Maurice Wilhelmus van der Heijden
  • DOUBLE-STRANDED RIBONUCLEIC ACID FOR ADJUVANTS
    Publication number: 20180044678
    Abstract: The invention provides a double-stranded ribonucleic acid (dsRNA) having a chain length suitable for simultaneously showing low toxicity and high function in the use of an adjuvant and the like, and resisting variation of chain length even when subjected to a heating and cooling treatment, or a salt thereof; an immune potentiator, adjuvant, pharmaceutical product and the like containing the dsRNA and the like; and a production method of such dsRNA and the like. The invention is characterized in that the weight average chain length of two or more single-stranded ribonucleic acids (ssRNAs) constituting the first chain constituting dsRNA is not more than ½ of the weight average chain length of one ssRNA constituting the second chain.
    Type: Application
    Filed: October 24, 2017
    Publication date: February 15, 2018
    Applicant: KYOWA HAKKO BIO CO., LTD.
    Inventors: Tetsuo NAKANO, Eitora YAMAMURA
  • COMPOSITION FOR TREATING CANCER ASSOCIATED WITH HPV INFECTION
    Publication number: 20180044679
    Abstract: The present invention relates to a composition for preventing or treating diseases associated with human papillomavirus (HPV), and more specifically, cancer associated with HPV, and even more specifically, cervical cancer. The nucleotide sequence of the present invention, the sequence in which the base thereof is modified, and a specific combination thereof can be useful in a composition for effectively treating diseases associated with HPV infection by greatly inhibiting the expression of the E6/E7 gene of HPV type 16 or 18.
    Type: Application
    Filed: June 26, 2017
    Publication date: February 15, 2018
    Inventors: Young Kee SHIN, Young Deug KIM, Hun Soon JUNG, Deuk Ae KIM
  • METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF KRAS BY ASYMMETRIC DOUBLE-STRANDED RNA
    Publication number: 20180044680
    Abstract: This invention relates to compounds, compositions, and methods useful for reducing KRAS target RNA and protein levels via use of Dicer substrate siRNA (DsiRNA) agents possessing asymmetric end structures.
    Type: Application
    Filed: November 1, 2017
    Publication date: February 15, 2018
    Inventor: Bob D. Brown
  • METHODS OF TREATING CANCER HARBORING HEMIZYGOUS LOSS OF TP53
    Publication number: 20180044681
    Abstract: Provided herein are methods of treating a patient having a cancer that exhibits (i) a hemizygous loss of the TP53 gene; (ii) a hemizygous loss of the POLR2A gene; and/or (iii) a decreased level of expression of a POLR2A gene product relative to a reference (i.e., control) expression level. The methods comprise administering a therapeutically effective amount of a POLR2A inhibitor (e.g., a nucleic acid that inhibits the expression of a POLR2A protein, an amatoxin, alpha-amanitin, or alpha-amanitin conjugated to a cell targeting moiety, such as an EpCAM antibody) to a patient having or determined to have (i) a hemizygous loss of the TP53 gene; (ii) a hemizygous loss of the POLR2A gene; and/or (iii) a decreased level of expression of a POLR2A gene product relative to a reference (i.e., control) level.
    Type: Application
    Filed: March 3, 2016
    Publication date: February 15, 2018
    Inventors: Xiongbin LU, Yunhua LIU
  • TREATMENT OF HYPERHIDROSIS
    Publication number: 20180044682
    Abstract: The present invention relates to a composition for reducing sweating in humans, characterized in that said composition comprises a compound capable of reduction of ITPR2 protein function and reduction of levels of ITPR2 mRNA and/or ITPR2 protein, and optionally pharmaceutically acceptable carriers and/or excipients, as well as to methods of treatment and specific siRNA molecules and their use in therapy.
    Type: Application
    Filed: September 19, 2017
    Publication date: February 15, 2018
    Inventor: Niklas Dahl
  • METHOD FOR PRODUCING COENZYME AND TRANSFORMANT SET FOR COENZYME PRODUCTION
    Publication number: 20180044683
    Abstract: The present invention provides a method for synthesizing NAD+ or NADH in an enzyme reaction system requiring NAD+ or NADH so as to supplement NAD+ lost due to thermal decomposition in the enzyme reaction system, and a transformant set for coenzyme production used in the method. That is, the present invention relates to a method for producing a coenzyme including performing synthesis of NAD+ or NADH in an enzyme reaction system requiring NAD+ or NADH by adding one or more thermostable enzymes required for a reaction for synthesizing NAD+ from nicotinamide, to the enzyme reaction system, and a transformant set for coenzyme production including one or more transformants into which one or more genes encoding thermostable enzymes required for a reaction for synthesizing NAD+ or NADH from nicotinamide are introduced by using a non-thermostable microorganism as a host.
    Type: Application
    Filed: February 19, 2016
    Publication date: February 15, 2018
    Applicant: Japan Science and Technology Agency
    Inventors: Kohsuke HONDA, Haruyuki ATOMI
  • COMPOSITIONS AND METHODS FOR 3-HYDROXYPROPIONIC ACID PRODUCTION
    Publication number: 20180044684
    Abstract: The present application discloses genetically modified yeast cells comprising an active 3-HP fermentation pathway, and the use of these cells to produce 3-HP.
    Type: Application
    Filed: August 28, 2017
    Publication date: February 15, 2018
    Inventors: Holly Jessen, Brian Rush, Jeanette Huryta, Beth Mastel, Alan Berry, Debbie Yaver, Michael Catlett
  • APPLICATION OF SYK SERVING AS THERAPEUTIC TARGET FOR HEPATIC FIBROSIS/HEPATIC CIRRHOSIS
    Publication number: 20180044685
    Abstract: The present invention discloses an application of SYK serving as a therapeutic target for hepatic fibrosis/hepatic cirrhosis. Through experiments, the investor has found that a SYK gene has a higher expression during the process of hepatic fibrosis/hepatic cirrhosis, and the progression of hepatic fibrosis is accelerated by promoting activation of the hepatic stellate cells (HSCs); and the progression of hepatic fibrosis/hepatic cirrhosis can be effectively slowed down by using a SYK inhibitor or by interfering with SYK gene expression, both of which have good therapeutic effects.
    Type: Application
    Filed: August 11, 2016
    Publication date: February 15, 2018
    Inventor: Jian HONG
  • TOOLS AND METHODS FOR USING CELL DIVISION LOCI TO CONTROL PROLIFERATION OF CELLS
    Publication number: 20180044686
    Abstract: The present disclosure provides molecular tools, methods and kits for using cell division loci (CDLs) to control cell proliferation in animal cells. CDLs, as provided herein, are loci whose transcription product(s) are expressed during cell division. CDLs may be genetically modified, as described herein, to comprise a negative selectable marker and/or an inducible activator-based gene expression system, which allows a user to permit, ablate, and/or inhibit proliferation of the genetically modified cell(s) by adding or removing an appropriate inducer.
    Type: Application
    Filed: March 9, 2016
    Publication date: February 15, 2018
    Inventors: Andras NAGY, Claudio MONETTI, Qin LIANG
  • ARTIFICIAL NUCLEIC ACID MOLECULES FOR IMPROVED PROTEIN EXPRESSION
    Publication number: 20180044687
    Abstract: The invention relates to an artificial nucleic acid molecule comprising an open reading frame and a 3?-UTR comprising at least one poly(A) sequence or a polyadenylation signal. The invention further relates to a vector comprising the artificial nucleic acid molecule comprising an open reading frame and a 3?-UTR comprising at least one poly(A) sequence or a polyadenylation signal, to a cell comprising the artificial nucleic acid molecule or the vector, to a pharmaceutical composition comprising the artificial nucleic acid molecule or the vector and to a kit comprising the artificial nucleic acid molecule, the vector and/or the pharmaceutical composition. The invention also relates to a method for increasing protein production from an artificial nucleic acid molecule and to the use of a 3?-UTR for a method for increasing protein production from an artificial nucleic acid molecule.
    Type: Application
    Filed: December 11, 2015
    Publication date: February 15, 2018
    Inventors: Andreas THESS, Thomas SCHLAKE, Stefanie GRUND
  • CORYNEFORM BACTERIUM TRANSFORMANT AND PROCESS FOR PRODUCING ORGANIC COMPOUND USING THE SAME
    Publication number: 20180044688
    Abstract: A coryneform bacterium transformant engineered by the following (A) to (D): (A) enhancement of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase activity; (B) prevention, inhibition, or reduction of intracellular sugar uptake mediated by phosphotransferase system (PTS); (C) enhancement of intracellular sugar uptake activity mediated by a sugar transporter different from phosphotransferase system and enhancement of glucokinase activity; and (D) enhancement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity is capable of efficiently producing shikimic acid or the like from a sugar.
    Type: Application
    Filed: August 20, 2015
    Publication date: February 15, 2018
    Applicant: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH
    Inventors: Masayuki INUI, Masako SUDA, Kazumi HIRAGA, Takahisa KOGURE
  • BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID
    Publication number: 20180044689
    Abstract: The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production.
    Type: Application
    Filed: July 20, 2017
    Publication date: February 15, 2018
    Inventors: Tom BEARDSLEE, Stephen PICATAGGIO, Alex HUTAGALUNG, Tom FAHLAND
  • METHODS AND COMPOSITIONS FOR GENE EXPRESSION IN PLANTS
    Publication number: 20180044690
    Abstract: The invention provides recombinant DNA molecules useful for providing efficient expression of proteins in transgenic plants, as well as compositions and methods for using the recombinant DNA molecules. In particular embodiments, the invention provides recombinant DNA molecules and constructs comprising sequences encoding transit peptides and operably linked sequences conferring herbicide tolerance.
    Type: Application
    Filed: July 26, 2017
    Publication date: February 15, 2018
    Inventors: Clayton T. Larue, Joel E. Ream, Aabid Shariff, Yuanji Zhang, Xuefeng Zhou
  • PLANT REGULATORY SEQUENCE
    Publication number: 20180044691
    Abstract: The present invention relates to regulatory sequences. In particular, the invention relates to a regulatory nucleic acid molecule, at least part of which has a transcription initiation function directing expression of an operably associated protein encoding polynucleotide of interest to non-tassel tissue in maize, but not or substantially not to tassel. The invention further relates to chimeric genes and expression cassettes comprising the regulatory nucleic acid molecule and to transgenic plants comprising the chimeric genes and expression cassettes.
    Type: Application
    Filed: October 5, 2017
    Publication date: February 15, 2018
    Applicant: Syngenta Participations AG
    Inventors: Mikyong LEE, Michael L. NUCCIO, Joseph CLARKE
  • COLLAGEN PRODUCING PLANTS AND METHODS OF GENERATING AND USING SAME
    Publication number: 20180044692
    Abstract: A method of producing collagen in a plant and plants producing collagen are provided. The method is effected by expressing in the plant at least one type of a collagen alpha chain in a manner enabling accumulation of the collagen alpha chain in a subcellular compartment devoid of endogenous P4H activity, thereby producing the collagen in the plant.
    Type: Application
    Filed: October 3, 2017
    Publication date: February 15, 2018
    Applicant: CollPlant Ltd.
    Inventors: Oded SHOSEYOV, Hanan STEIN
  • LIMITED TRANSPIRATION TRAITS AND BREEDING METHODS IN MAIZE
    Publication number: 20180044693
    Abstract: Methods to improve drought tolerance and increasing yield by the application limited transpiration trait in maize plants are provided. Expression of the limited transpiration trait in maize under increasing vapor pressure deficit conditions provide positive impact on overall crop yield, depending on environmental conditions.
    Type: Application
    Filed: March 4, 2016
    Publication date: February 15, 2018
    Applicants: PIONEER HI-BRED INTERNATIONAL, INC., NORTH CAROLINA STATE UNIVERSITY
    Inventors: MARK COOPER, CARLOS MESSINA, THOMAS SINCLAIR, GRAEME HAMMER
  • DOWN-REGULATION OF A HOMEODOMAIN-LEUCINE ZIPPER I-CLASS HOMEOBOX GENE FOR IMPROVED PLANT PERFORMANCE
    Publication number: 20180044694
    Abstract: Methods for modulating plants using optimized ZmME293 down-regulation constructs are disclosed. Also disclosed are nucleotide sequences, constructs, vectors, and modified plant cells, as well as transgenic plants displaying increased seed and/or biomass yield, improved tolerance to abiotic stress such as drought or high plant density, improved nitrogen utilization efficiency, increased ear number and/or reduction in time to scenescence.
    Type: Application
    Filed: May 19, 2017
    Publication date: February 15, 2018
    Inventors: Mei Guo, Xiaomu Niu, Mary Rupe, Jeffrey R. Schussler
  • Method of Increasing Resistance Against Soybean Rust in Transgenic Plants by ADR-1-Gene
    Publication number: 20180044695
    Abstract: The present invention relates to a method of increasing resistance against soybean rust in transgenic plants and/or plant cells. In these plants, the content and/or the activity of an ADR-1-protein are increased in comparison to the wild-type plants not including a recombinant ADR-1-gene.
    Type: Application
    Filed: August 21, 2017
    Publication date: February 15, 2018
    Applicant: Basf Plant Science Company GMBH
    Inventor: Holger Schultheiss
  • PHACOSPORACEA RESISTANT SOYBEAN PLANTS
    Publication number: 20180044696
    Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the family Phacosporaceae in transgenic plants and/or plant cells. In these plants, the ethylene signaling pathway and/or activity of the ethylene signaling compounds is changed. This is achieved by priming the ethylene signaling pathway in these plants in comparison to wild type plants and/or wild type plant cells. Depending on the activating or inhibitory function of a particular signaling compound overexpression or knock-down of the cognate gene might be used.
    Type: Application
    Filed: August 30, 2017
    Publication date: February 15, 2018
    Inventor: Holger Schultheiss
  • Methods and Compositions for Goss' Wilt Resistance in Corn
    Publication number: 20180044697
    Abstract: The present invention relates to the field of plant breeding. More specifically, the present invention includes a method of using haploid plants for genetic mapping of traits of interest such as disease resistance. Further, the invention includes a method for breeding corn plants containing quantitative trait loci (QTL) that are associated with resistance to Goss' Wilt, a bacterial disease associated with Clavibacter michiganense spp.
    Type: Application
    Filed: October 24, 2017
    Publication date: February 15, 2018
    Inventors: Michael R. Kerns, Hongwu Jia, David Butruille, Travis J. Frey, Kevin Cook, Laron Peters
  • SITE-SPECIFIC INTEGRATING RECOMBINANT AAV VECTORS FOR GENE THERAPY AND IMPROVED PRODUCTION METHODS
    Publication number: 20180044698
    Abstract: Provided herein are methods of site-specific integration of a heterologous sequence into a host genome (e.g., by administering a recombinant adeno-associated virus (rAAV) to a host cell in the presence of a Rep protein), and methods for treating diseases and disorders by delivering an rAAV that comprises a nucleic acid vector comprising a Rep protein. Also provided herein are methods and compositions for producing rAAV particles with improved titer and transduction efficiencies.
    Type: Application
    Filed: February 19, 2016
    Publication date: February 15, 2018
    Applicant: University of Florida Research Foundation, Incorporated
    Inventors: Arun Srivastava, Chen Ling
  • NUCLEIC ACID-CONTAINING LIPID PARTICLES AND RELATED METHODS
    Publication number: 20180044699
    Abstract: Lipid particles containing a nucleic acid, devices and methods for making the lipid particles, and methods for using the lipid particles.
    Type: Application
    Filed: August 25, 2017
    Publication date: February 15, 2018
    Applicant: The University of British Columbia
    Inventors: Pieter R. Cullis, Nathan M. Belliveau, Carl Lars Genghis Hansen, Jens Huft, Robert James Taylor, Andre Wild, Stuart Malcolm, Ismail Hafez, Alex Leung, David Walker
  • METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION
    Publication number: 20180044700
    Abstract: The present disclosure provides compositions and methods of site-specific modification of a target DNA, or a protein associated with a target DNA, in a eukaryotic cell. The present disclosure provides methods of binding a target DNA in a eukaryotic cell.
    Type: Application
    Filed: September 1, 2015
    Publication date: February 15, 2018
    Inventors: Jennifer A. DOUDNA, Steven LIN, Brett T. STAAHL
  • BACILLUS MUCILAGINOSUS AND HIGH-DENSITY FERMENTATION METHOD AND USE THEREOF
    Publication number: 20180044701
    Abstract: This invention discloses a mutated strain of wild type Bacillus mucilaginosus HSCUP-76-8 and a high-density fermentation method thereof. The mutated strain HSCUP-76-8 was assigned an Accession No. CGMCC No. 8481. A two-stage and regulated high-density fermentation method has been established for the production of HSCUP-76-8. In the first stage, parameters are controlled to reduce the viscosity of the fermentation broth and promote the growth of bacteria, thereby allowing the bacteria to reach an amount in the range of 2.0×109 cfu/mL-2.3×109 cfu/ml. In the second stage, nutritional factors and fermentation conditions are controlled to promote sporulation, thereby producing endospores in the range of 1.5×109 cfu/mL-2.0×109 cfu/ml. The fermentation cycle of the two-stage high-density fermentation is 32-48 hours.
    Type: Application
    Filed: July 6, 2015
    Publication date: February 15, 2018
    Inventors: Yaogui MU, Liangqi ZHAO, Wenwang QIN, lIHUA LV, Qi CHEN
  • METHOD AND APPARATUS FOR PRODUCING ORGANIC SUBSTANCES
    Publication number: 20180044702
    Abstract: It is an object of the present invention to convert CO2 to CO by bringing about a reverse shift reaction in a relatively low-temperature condition, and thereby enhancing efficiency of generating organic substances, such as ethanol. A raw material gas g1 containing CO2 and H2 is subjected to a reverse shift reaction in a reverse shift reactor 12. Organic substances are generated from a raw material gas g2 after the reverse shift reaction in an organic substance generator 13. In the subjecting step, the raw material gas g1 is brought into contact with a reverse shift reaction catalyst 20. The reverse shift reaction catalyst 20 includes a support 21 and a catalyst metal 22 supported by the support 21. The catalyst metal 22 includes a transition metal. The catalyst metal 22 is preferably composed of Fe with at least one kind of metal from among Al, Ga, In, Cu, Ag, Au, Pd and Mn added thereto.
    Type: Application
    Filed: March 17, 2016
    Publication date: February 15, 2018
    Applicant: SEKISUI CHEMICAL CO., LTD.
    Inventors: Kanetomo SATOU, Rasika DASANAYAKE ALUTHGE
  • Method for Producing an Acidic Substance Having a Carboxyl Group
    Publication number: 20180044703
    Abstract: An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.
    Type: Application
    Filed: October 19, 2017
    Publication date: February 15, 2018
    Applicant: AJINOMOTO CO., INC.
    Inventors: Yoshihiko Hara, Keita Fukui, Yoshinori Tajima, Kazue Kawamura, Yoshihiro Usuda, Kazuhiko Matsui
  • Methods For Enhancing The Degradation Or Conversion Of Cellulosic Material
    Publication number: 20180044704
    Abstract: The present invention relates to methods for degrading or converting a cellulosic material and for producing substances from the cellulosic material under high temperature conditions.
    Type: Application
    Filed: September 28, 2017
    Publication date: February 15, 2018
    Applicant: Novozymes, Inc.
    Inventors: Tarana Shaghasi, Brett McBrayer
  • COOLING AND PROCESSING MATERIALS
    Publication number: 20180044705
    Abstract: Systems and methods for cooling and processing materials are disclosed.
    Type: Application
    Filed: October 23, 2017
    Publication date: February 15, 2018
    Inventor: Marshall Medoff
  • Multi-Stage Enzymatic Hydrolysis of Lignocellulosic Biomass Employing An Oxidoreductase with an AA9 Polypeptide
    Publication number: 20180044706
    Abstract: The invention relates to processes of multi-stage hydrolysis where different enzyme compositions are added in at least two stages of hydrolysis. In a first stage, a first enzyme composition of one or more cellulolytic enzymes and at least one of an oxidoreductase and an AA9 polypeptide having cellulolytic enhancing activity is added, followed by a latter stage in which a second enzyme composition comprising one or more cellulases is added. Also provided are processes for obtaining hydrolysis products and fermentation products using processes of the invention.
    Type: Application
    Filed: March 11, 2016
    Publication date: February 15, 2018
    Applicant: Novozymes A/S
    Inventors: Jiyin Liu, James Michael Broering, Keith McFarland, Xin Li
  • Multi-Stage Enzymatic Hydrolysis of Lignocellulosic Biomass
    Publication number: 20180044707
    Abstract: The invention relates to processes of multi-stage hydrolysis where different enzyme compositions are added in at least two stages of hydrolysis. In a first stage, a first enzyme composition of xylanase, a beta-xylosidase and an endoglucanase is added, followed by a latter stage in which a second enzyme composition, a cellulolytic enzyme composition, is added. Also provided are processes for obtaining hydrolysis products and fermentation products using processes of the invention.
    Type: Application
    Filed: March 11, 2016
    Publication date: February 15, 2018
    Applicants: Novozymes A/S, Beta Renewables Spa
    Inventors: Jesper Frickmann, Johan Belfrage, Eric Abbate, David Osborn, Geoffrey Moxley, Daniele Riva, Piero Otonello, Simone Ferrero, Micol Purrotti, Stefano Paravisi, Chiara Prefumo
  • Methods for the Enzymatic Modification of Steviol Glycosides, Modified Steviol Glycosides Obtainable Thereby, and the Use Thereof as Sweeteners
    Publication number: 20180044708
    Abstract: The present invention relates generally to the production of steviol glycosides. Provided is a method for enzymatically providing a modified steviol glycoside, comprising incubating a steviol glycoside substrate in A the presence of sucrose and the glucansucrase GTF180 of Lactobacillus reuteri strain 180, or a mutant thereof having the desired transglycosylation activity. Also provided are modified steviol glycosides obtainable by a method of the invention, and the use thereof as low-glycemic sweetener.
    Type: Application
    Filed: March 10, 2016
    Publication date: February 15, 2018
    Inventors: Evelien Maria te Poele, Lubbert Dijkhuizen, Gerrit Johannes Gerwig, Johannis Paulus Kamerling
  • USE OF PHENOLIC ANTIOXIDANTS IN CELL CULTURE FOR THE PRODUCTION OF PROTEINS
    Publication number: 20180044709
    Abstract: The present invention relates to new methods and processes for culturing mammalian cells with the addition of phenolic antioxidants. Performance of the cell culturing methods and processes in their various aspects result in a higher viable cell density and higher protein titer.
    Type: Application
    Filed: February 11, 2016
    Publication date: February 15, 2018
    Inventor: Jun Tian
  • METHOD FOR PRODUCING GLUTATHIONE
    Publication number: 20180044710
    Abstract: The present invention aims to provide a yeast that is genetically modified so as to more highly produce glutathione, and a method of producing glutathione utilizing the yeast. The present invention provides a method of producing glutathione, including culturing a yeast whose thiol oxidase activity is increased as compared to the parent strain in a culture medium to produce glutathione, and recovering glutathione from the cultured broth obtained.
    Type: Application
    Filed: March 4, 2016
    Publication date: February 15, 2018
    Applicants: KANEKA CORPORATION, NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Kiyotaka HARA, Akihiko KONDO, Akira IWASAKI, Yuichi IWAMOTO
  • LYSINE CONJUGATED IMMUNOGLOBULINS
    Publication number: 20180044711
    Abstract: Provided herein are conjugated immunoglobulins and methods for generating conjugated immunoglobulins using a microbial transglutaminase.
    Type: Application
    Filed: July 28, 2017
    Publication date: February 15, 2018
    Inventors: Jared Spidel, Earl Albone
  • Methods, Apparatuses, and Systems for Analyzing Microorganism Strains from Complex Heterogeneous Communities, Predicting and Identifying Functional Relationships and Interactions Thereof, and Selecting and Synthesizing Microbial Ensembles Based Thereon
    Publication number: 20180044712
    Abstract: Methods, apparatuses, and systems for screening, analyzing and selecting microorganisms from complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and synthesizing microbial ensembles based thereon are disclosed. Methods for identifying and determining the absolute cell count of microorganism types and strains, along with identifying the network relationships between active microorganisms and environmental parameters, are also disclosed.
    Type: Application
    Filed: October 23, 2017
    Publication date: February 15, 2018
    Applicant: Ascus Biosciences, Inc.
    Inventor: Mallory EMBREE
  • LEUKOCYTE ESTERASE DETECTION FROM THROAT SWAB
    Publication number: 20180044713
    Abstract: The present invention relates to the field of infectious diseases. The invention specifically relates to the diagnostic test for acute bacterial pharyngitis. The test is used in screening for Group A Beta Haemolytic Streptococcus by the identifying the presence of leukocyte esterase in the throat. The Leukocyte Esterase Throat Swab Test is compared to the Rapid Step Test for efficiency in terms of fast delivery of results.
    Type: Application
    Filed: September 27, 2017
    Publication date: February 15, 2018
    Inventor: Kumara Venkatanarayana Nibhanipudi