Abstract: Disclosed are components and systems for cell-free glycoprotein synthesis (CFGpS). In particular, the components and systems include and utilize prokaryotic cell lysates from engineered prokaryotic cell strains that have been engineered to enable cell-free synthesis of glycoproteins.

Abstract: In this disclosure, transition and rare earth metal-based magnetic ionic liquids (MILs) are successfully prepared in a two-step synthesis and used to extract viable bacteria from a liquid sample. The disclosed MILs are extremely hydrophobic MILs and were insoluble in aqueous solution at 0.01% (v/v). Furthermore, these MILs were miscible in a variety of polar and non-polar organic solvents. Moreover, these MILs possess low viscosity and increased magnetic susceptibility. These MILs possess unique characteristics that can have great potential uses in various chemical applications such as extraction solvents in LLE, liquid electrochromic materials (Co-based MILs), and novel reaction media for organic synthesis.

Abstract: A method and automated apparatus for rapid non-invasive detection of a microbial agent in a test sample is described herein. The apparatus may include one or more means for automated loading, automated transfer and/or automated unloading of a specimen container. The apparatus also includes a detection system for receiving a detection container, e.g., container or vial, containing a biological sample and culture media. The detection system may also include one or more heated sources, holding structures or racks, and/or a detection unit for monitoring and/or interrogating the specimen container to detect whether the container is positive for the presence of a microbial agent therein. In other embodiment, the automated instrument may include one or more, bar code readers, scanners, cameras, and/or weighing stations to aid in scanning, reading, imaging and weighing of specimen containers within the system.

Abstract: Apparatus for automated venting and/or sampling of a specimen container having a closure sealing the interior of the specimen container from the environment is disclosed. The apparatus includes a rack holding the specimen container; a venting and/or sampling device having a needle, a chamber in fluid communication with the needle and a port in fluid communication with the chamber; a robotic transfer mechanism movable relative to the rack; a sample removal apparatus attached to the robotic transfer mechanism having gripping features for gripping the venting device.

Abstract: The present invention relates to a method for detecting or quantifying CTP in a cell sample comprising at least two nucleotide triphosphates by cationic ion pairing chromatography coupled to mass spectrometry, to a method for detecting or quantifying CTP synthase activity based on the method for detecting or quantifying CTP, and to their use in methods for screening potential immunosuppressive or anti-cancer compounds and in methods for determining the appropriate dose of an immunosuppressive or anti-cancer compound inhibiting CTP synthase activity for a treated subject.

Abstract: Provided herein are methods and compositions for depleting targeted nucleic acid sequences from a sample, enriching for sequences of interest from a sample, and/or partitioning of sequences from a sample. The methods and compositions are applicable to biological, clinical, forensic, and environmental samples.

Abstract: The present invention discloses a novel polypeptide capable of binding Taq DNA polymerase, a polynucleotide encoding the polypeptide, a recombinant vector comprising the polynucleotide, a cell having the recombinant vector introduced therein, a method of producing the polypeptide using the recombinant cell, and a hot-start PCR composition comprising the polypeptide. The polypeptide of the present invention is capable of binding specifically to Taq DNA polymerase to inhibit the activity of the polymerase, and thus may be effectively used in hot-start PCR using Taq DNA polymerase.

Abstract: Methods for predicting clinical outcome for a human subject diagnosed with squamous cell lung carcinoma using a panel of molecular markers that includes CDKN2A and CCND1. The markers are related to the subject's increased likelihood of a negative clinical outcome.

Abstract: Systems and method for validation of sequencing results can amplify a target region of a nucleic acid sample in the presence of a primer pool including target specific and variant specific primers. The variant specific primers can include variant specific barcodes and variant specific sequences. An amplicon can be sequenced to determine the sequence of the variant specific barcode. The variant can be identified based on the sequence of the variant specific barcode, and the location of the variant can be determined by mapping the amplicon to a reference sequence.

Abstract: Focused acoustic treatment of samples may be used to differentially shear different fragment lengths of DNA or other nucleic acid portions of a sample. Relatively larger fragment lengths may be sheared while smaller fragment lengths are unaffected by the focused acoustic based shearing.

Abstract: A processing module is configured to extend the capabilities of an analyzer configured to process substances within each of a plurality of receptacles. The module includes a container transport configured to transport a container from a location within the processing module to a location within the analyzer that is accessible to a substance transfer device of the analyzer. A receptacle distribution system is configured to receive a receptacle from the analyzer, transfer the receptacle into the processing module, and to move the receptacle between different locations within the analyzer. A substance transfer device of the module is configured to dispense substances into or remove substances from the receptacle within the processing module. A reagent card exchanger provides an input device for inserting reagent cards into and removing reagent cards from the module, stores reagent cards within the module, and transfers reagent cards to different location within the module.

Abstract: One or more temperature cycles are applied to the contents of a processing vial closed by an interlocked cap within a thermal cycler. The processing vial and the interlocked cap are transferred from the thermal cycler to a storage compartment within the instrument with a transfer mechanism with a probe engaged with the cap. In the storage compartment, the processing vial and interlocked cap are moved to an access opening in the instrument and are removed from the instrument through the access opening.

Abstract: This invention relates to a method of detecting a gene. The invention also relates to a method of determining the Amplification expression level of a gene. The invention also relates to compositions for use in these methods.

Abstract: The present invention relates to low-density biochips and methods for rapid detection of bacterial organisms involved in microbially influenced corrosion (MIC).

Abstract: Described herein are techniques and methods to analyze mutations in genomic DNA. The techniques and methods can be used to diagnose disease in a subject.

Abstract: The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

Abstract: Method and system for quantifying target nucleic acids using real-time amplification and internal calibration adjustment. The approach employs a single fixed data point in combination with a single adjustment calibrator amplified on the instrument that is to be calibrated for approximating a complete calibration curve.

Abstract: Provided herein are multiplex methods for detecting the presence or absence and amount of variants of a plurality of target nucleic acid species having low-abundance variants and high-abundance variants.

Abstract: In some aspects, the present disclosure provides methods for enriching amplicons, or amplification products, comprising a concatemer of at least two or more copies of a target polynucleotide. In some embodiments, a method comprises sequencing the amplicons comprising at least two or more copies of a target polynucleotide. In some embodiments, the target polynucleotides comprise sequences resulting from chromosome rearrangement, including but not limited to point mutations, single nucleotide polymorphisms, insertions, deletions, and translocations including fusion genes. In some aspects, the present disclosure provides compositions and reaction mixtures useful in the described methods.

Abstract: The present invention was made to provide novel methods for detecting DNA and devices therefor. Specifically, the present invention is a method of detecting DNA including the steps of: adding, to an oil, a fluorescently-labeled probe, a DNA intercalator, and a DNA solution containing a target DNA to produce droplets; performing PCR on the droplets; and measuring fluorescence from the fluorescently-labeled probe and fluorescence from the DNA intercalator, wherein the DNA solution has a concentration at which each droplet is produced so as to contain one or less target DNA molecule. In addition, the present invention is a DNA detection device including a droplet production unit, a thermal cycler unit, and a fluorescence detection unit which perform these steps.

Abstract: The present disclosure provides, in one aspect, a device and a method for unit sequencing and/or analysis of a molecular sequence comprising attaching the molecular sequence to a plate and controlling the progression of the molecular sequence through a pore of a nanopore chip, wherein the separation distance between the nanopore chip and the scan plate is controlled by a precision mechanical drive, and the molecular sequence is sensed as it progresses through the nanopore.

Abstract: The object is to provide a method that enables detection of unknown virus sequences and efficient detection and search of viruses. The method comprises the step of randomly fragmenting an objective double-stranded (ds) RNA to obtain dsRNA fragments; the step of subjecting the obtained dsRNA fragments to a reverse transcription reaction and then performing polymerase chain reaction (PCR) to obtain corresponding DNA fragments; and the step of subjecting the obtained DNA fragments to a sequence analysis operation to determine the sequences. The reverse transcription reaction is preferably started from the 3? ends of the dsRNA fragments.

Abstract: The invention generally relates to sequencing library preparation methods. In certain embodiments, two template nucleic acids are joined together by a linking molecule, such as a PEG derivative. The linked template nucleic acids is amplified, creating linked amplicons.

Abstract: Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.

Abstract: The invention generally relates to methods for analyzing nucleic acids to identify novel mutations associated with diseases. In certain embodiments, methods of the invention involve obtaining nucleic acid from a subject having a disease, identifying at least one mutation in the nucleic acid, and comparing the mutation to a database of mutations known to be associated with the disease, wherein mutations that do not match to the database are identified as novel mutations.

Abstract: Embodiments in accordance with the present disclosure are directed to assessing for the presence of different target sequences in a sample. Embodiments include providing a binary result of the presence or absence of target sequences that is indicative of a disease or other physiological condition. An example method includes exposing a sample to a plurality of probes, the plurality of probes including a plurality of complimentary sequences that bind to a plurality of target sequences in the sample, and a plurality of different tag sequences for each of the plurality of target sequences in the sample. At least a portion of the target sequences bound to the probes are caused to bind to the different locations on the substrate. And, the method includes, by using scanning circuitry and information indicative of the different locations and associated tag sequences, assessing the number of the target sequences in the sample.

Abstract: Disclosed herein are a method and a kit for making a diagnosis as to whether a subject suffers from myocardial infarction (MI). The method and the kit can accurately and efficiently identify the MI patient through detecting the circulating let-7a and let-7f, both of which are highly expressed in healthy subject, and downregulated in MI patient. According to the present method and kit, the MI patient is capable of receiving a proper treatment in time.

Abstract: The present invention provides a method for treating a human subject afflicted with a metabolic disease with a pharmaceutical composition, comprising the steps of: (i) determining JAG1 expression level in the subject; (ii) identifying the subject as a predicted responder if JAG1 expression level is greater than 1.5 fg/ng 18S; and (iii) administering the pharmaceutical composition to the subject only if the subject is identified as a predicted responder.

Abstract: Genes whose expression is correlated with the presence of CIDP or vasculitic neuropathy are disclosed. Probes and sets of nucleic acids and proteins specific for these genes are described, as are molecular and immunological methods for aiding in the diagnosis of these disease conditions in a subject.

Abstract: The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect both DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

Abstract: The invention provides a collection of gene expression signatures that is linked to longevity. Also provided herein are assays, methods, and systems for identifying a treatment that can modulate lifespan or determining an effect of a treatment on a health profile of a subject by using the collection of gene expression signatures described herein.

Abstract: The present invention relates to the field of pharmacogenomics and in particular to assessing the response of a cancer patient to a treatment by analysing CpG methylation in the shox2 gene. Depending on the result of the analysis, the treatment can be continued or altered, thereby exploiting the therapeutic window better than conventional methods of assessing a treatment response.

Abstract: The present disclosure is a method comprising the steps of performing one or more nucleic-acid based assays to identify mutations present in the breast tissue acquired from the subject corresponding to a first test module and a second test module associated with detection of at least one predetermined mutation of one or more genes, wherein each test module is configured to provide a positive outcome corresponding to at least one predetermined mutation detected in the tissue or a negative outcome corresponding to absence of detectable predetermined mutation in the sample; and identifying the type of neoplasm of the breast tissue based upon the provided outcome of the both test modules. Preferably, the first test module is associated with detection of mutation in MED12 gene and/or RARA gene, while the second test module is associated with detection of mutation in FLNA gene, SETD2 gene and/or MLL2 gene.

Abstract: A method for predicting the likelihood of locoregional recurrence (LRR) and/or distant metastasis in a subject with breast cancer following mastectomy and/or breast conserving surgery, comprising: measuring the expression level of at least one gene in a sample isolated from the subject; and deriving a score based on the measured expression level of the at least one gene, wherein the at least one gene is selected from a group consisting of TRPV6, DDX39, BUB1B, CCR1, STIL, BLM, C16ORF7, TPX2, PTI1, TCF3, CCNB1, DTX2, PIM1, ENSA, RCHY1, NFATC2IP, OBSL1, and MMP15, and wherein the derived score provides an indication of the likelihood of LRR and/or the likelihood of distant metastasis in the subject.

Abstract: The present invention includes methods for detecting cancer in a subject and measuring the effectiveness of a cancer treatment. In certain embodiments, the invention includes assessing the level of unshielded RN7SL1 RNA in a sample from a subject.

Abstract: The present invention is based on BCR-ABL1 splice variants which result from insertion and/or truncation of the bcr-abl1 transcript and the finding that these variants provide resistance to kinase domain inhibitors such as imatinib, nilotinib and dasatinib.

Abstract: Embodiments of this invention include methods for detection of markers of bladder cancer and inflammatory conditions of the bladder. Particularly, methods include detection of expression of certain genetic markers for bladder cancer and markers for detection of inflammatory conditions of the bladder. These methods provide improved detection of the markers, and provide better detection of bladder cancer and inflammatory conditions of the bladder.

Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

Abstract: Herbicide resistant sunflower plants comprising two different herbicide-resistant alleles of the sunflower acetohydroxyacid synthase large subunit 1 (AHASL1) gene are described. Methods for making these sunflower plants and methods for controlling weeds or other undesired vegetation growing in the vicinity of these sunflower plants are disclosed. Such methods involve the use of acetohydroxyacid synthase-inhibiting herbicides. Methods for controlling parasitic weeds growing on sunflower plants are also described. Additionally provided are methods for determining the genotype of sunflower plants for AHASL1 gene.

Abstract: The present invention relates to methods for the identification of clinical risk in patients having, or suspected of having, influenza. The invention also relates to methods for distinguishing between patients having influenza or viral pneumonia from patients having a symptomatically similar condition. The methods of the invention comprise determination of the level of expression of interferon alpha inducible protein 27 (IFI27) in a biological sample from a patient having, or suspected of having, influenza. Kits comprising suitable components for the performance of the methods are also provided by the invention. The invention allows stratification of patients into groups defining clinical risk, for example groups based on the severity of risk to the long-term health of the subject.

Abstract: The present invention relates to glucoamylase variants having an increase in specific activity and/or an increase in melting temperature measured by TSA of at least 0.5 degrees Celsius, and further wherein the variants have at least at least 85%, at least 90%, at least 95% identity, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: Systems, apparatuses, and methods for producing and using a sugar cane syrup, juice, or powder. In one embodiment, the invention is directed to a system and associated processes for processing sugar cane using a cold processing pipeline in a manner that retains its natural nutritional value while producing a syrup, juice, or powder without significant separation of the natural nutrients or use of harmful chemicals.

Abstract: This invention pertains to plastic injection mold tooling, and also large forgings, formed from a low carbon mold steel having markedly increased hardening and hardenability properties in large sections as contrasted to currently available commercial products. The above attributes are obtained together with equal or better machinability and improved mold parting line wear. When manufactured in conjunction with a double melt process, this invention can improve significantly polishing characteristics and other attributes of molded parts in tooling sets.

Abstract: An online-control cooling process for seamless steel tube for effectively refining grains, comprising the following steps: when the temperature of a crude tube is higher than Ar3, evenly spraying water along the circumferential direction of the tube so as to continuously cool the tube to T1° C.˜T2° C., the cooling rate being controlled to be N1° C./s˜N2° C./s, wherein T1=810?360C?80(Mn+Cr)?37Ni?83Mo, T2=T1+115° C., N1=55-80×C, N2=168*(0.8?C), and C, Mn, Cr, Ni and Mo in the equations each represent the mass percentage of corresponding elements in the seamless steel tube; then, cooling to the room temperature at cooling rate no more than 10° C./s. Correspondingly, also provided are a method for manufacturing seamless steel tube for effectively refining grains, and a seamless steel tube. The online-control seamless steel tube cooling process does not require adding too many alloying elements, which is simple and can yield seamless steel tubes with good grain refinement and better toughness.

Abstract: Disclosed is, as a high-strength steel plate of API X100 grade or higher, a steel plate for structural pipes or tubes that exhibits high strength in a rolling direction and that has only a small difference between strength in a rolling direction and strength in a direction perpendicular to the rolling direction (exhibiting high material homogeneity) without addition of large amounts of alloying elements. The steel plate for structural pipes or tubes disclosed herein has: a specific chemical composition; a microstructure mainly composed of bainite and containing martensite austenite constituent in an area fraction of less than 3.0%; a tensile strength in the rolling direction of 760 MPa or more; and TSC?TSL being 30 MPa or less in terms of absolute value, where TSC denotes a tensile strength in a direction perpendicular to the rolling direction.

Abstract: Disclosed is, as a high-strength steel plate of API X80 grade or higher with a thickness of 38 mm or more, a steel plate for structural pipes or tubes that exhibits excellent resistance to PWHT and excellent toughness at the heat-affected zone, particularly at the butting faces joined by welding, without addition of large amounts of alloying elements. The steel plate for structural pipes or tubes disclosed herein has a specific chemical composition, in which the steel plate has mechanical properties including: (a) a 0.5% yield strength of 555 MPa or more; (b) a tensile strength of 625 MPa or more; and (c) a Charpy absorption energy vE?10° C. at ?10° C. at its mid-thickness part of 250 J or more, and maintains the mechanical properties (a) to (c) even after subjection to heat treatment at 650° C. for 2 hours.

Abstract: A steel sheet for galvannealed steel contains, by mass %, C: 0.25 to 0.70%, Si: 0.25 to 2.50%, Mn: 1.00 to 5.00%, Al: 0.005 to 3.50%, P: 0.15% or less, S: 0.03% or less, N £ 0.02%, O £ 0.01%, and optionally one or more selected from Ti, Nb, V, Cr, Mo, Cu, Ni, B, Ca, REM, Mg, W, Zr, Sb, Sn, As, Te, Y, Hf and Co, a balance being Fe and impurities. The microstructure consists of, by vol. %, retained g: 10.0 to 60.0%, high-temperature tempered martensite3 5.0%, low-temperature tempered martensite3 5.0%, fresh martensite £ 10.0%, a: 0 to 15.0%, P: 0 to 10.0%, a balance being bainite. Total volume ratio of tempered martensite and bainite is 30.0% or more, tensile strength is 1470 MPa or more, tensile strength×uniform elongation is 13000 MPa % or more, and tensile strength×local elongation is 5000 MPa % or more.

Abstract: An anti-hydrogen-induced-cracking pressure vessel steel plate containing, chemical composition of the steel plate according to the weight percentage: 0.16-0.20% of C, 0.15-0.40% of Si, 1.05-1.20% of Mn, less than or equal to 0.008% of P, less than or equal to 0.002% of S, less than or equal to 0.01% of Nb, less than or equal to 0.01% of V, less than or equal to 0.01% of Ti, less than or equal to 0.0005% of B, and the balance being Fe and inevitable impurity elements; carbon equivalent Ceq is less than or equal to 0.42%, and the computational formula for the carbon equivalent is Ceq=C+Mn/6+(Cr+Mo+V)/5+(Ni+Cu)/15.

Abstract: Disclosed are a non-quenched and tempered wire rod and a manufacturing method therefor, the non-quenched and tempered wire rod comprising in percentage by weight: 0.15 to 0.30% of C; 0.05 to 0.3% of Si; 1.0 to 2.0% of Mn; 0.5% of less of Cr (except for 0%); 0.02% or less of P; 0.02% or less of S; 0.01 to 0.05% of sol. Al; 0.005 to 0.02% of Nb; 0.05 to 0.2% of V; 0.01% or less of N; Fe as the remainder; and unavoidable impurities, wherein the non-quenched and tempered wire rod satisfies the following formulas 1 and 2, wherein, when the hardness of the wire rod measured in ½d position and in ¼d position in the diameter direction of the wire rod is Hv,1/2d(Hv) and Hv,1/4d(Hv), respectively (here, d is the diameter of the wire). (Hv,1/2d+Hv,1/4d)/2?240??[Formula 1] Hv,1/2d/Hv,1/4d?1.

Abstract: An object of the present invention is to provide a method for recovering gold in an ore or a refining intermediate by sufficiently leaching gold in a raw material resulting from the ore or the refining intermediate in an acidic solution containing a copper ion, an iron ion and a halide ion, which can contribute to improve the recovery rate of gold.