Patents Issued in July 25, 2019
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Publication number: 20190225969Abstract: The present invention relates to a recombinant vector, characterized by including a cytotoxic T-lymphocyte-associated protein-4 (CTLA-4)-targeting trans-splicing ribozyme expression cassette for delivery of chimeric antigen receptor, wherein the expression cassette includes: (i) a CTLA-4-targeting trans-splicing ribozyme; and (ii) a polynucleotide encoding a chimeric antigen receptor ligated to the 3? exon of the ribozyme. The present invention also relates to a transformed cell into which the recombinant vector is introduced, a ribozyme expressed from the recombinant vector, a retrovirus expressing the ribozyme, and a T cell treated with the retrovirus.Type: ApplicationFiled: February 2, 2016Publication date: July 25, 2019Inventors: Seong Wook Lee, Bit Na Yang, Sung Jin Kim, Seung Ryul Han
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Publication number: 20190225970Abstract: The present embodiments provide methods, compounds, and compositions for treating, preventing, or ameliorating a disease associated with excess growth hormone using antisense compounds or oligonucleotides targeted to growth hormone receptor (GHR).Type: ApplicationFiled: December 27, 2018Publication date: July 25, 2019Inventors: Sanjay BHANOT, Susan M FREIER, Huynh-hoa BUI
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Publication number: 20190225971Abstract: The present invention relates to a covalently closed circular recombinant DNA molecule comprising an origin of replication and an insert comprising a homopolymeric region, wherein the homopolymeric region is located at a distance of least 500 bp from the origin of replication in the direction of replication and/or wherein the insert comprising a homopolymeric region is oriented so that the direction of transcription of the insert is the same as the direction of replication of the origin of replication. The invention further relates to the use of the covalently closed circular recombinant DNA molecule for increasing the yield and/or shortening the fermentation time during fermentation.Type: ApplicationFiled: August 4, 2016Publication date: July 25, 2019Inventor: Jim WILLIAMS
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Publication number: 20190225972Abstract: Materials and methods are provided which allowed for increased expression of a transfected gene of interest in a recombinant host cell.Type: ApplicationFiled: August 30, 2018Publication date: July 25, 2019Inventor: Howard Robert Grahame Clarke
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Publication number: 20190225973Abstract: A Saccharomyces cerevisiae expression system and a construction method and application thereof, including an expression vector which includes, from 5? to 3?, a YEplac195 plasmid backbone, an exogenous gene expression cassette, and a selective marker gene expression cassette. The exogenous gene expression cassette includes from upstream to downstream an rDNA promoter, an internal ribosome entry site (IRES) sequence, an exogenous gene expression cassette, a poly(T) sequence, and an rDNA terminator. The selective marker gene expression cassette includes a promoter, a selective marker gene, and a transcription terminator.Type: ApplicationFiled: January 21, 2019Publication date: July 25, 2019Inventors: Xiaoming Bao, Lili Xu, Chenxi Qiu, Hongxing Li, Yong Yi, Jixiang Zhang, Chuanchao Fu, Dong Wang
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Publication number: 20190225974Abstract: Improved methods and means are provided to modify in a targeted manner the genome of a plant cell at a predefined site using a nucleotide-guided DNA modifying polypeptide such as a RNA-guided endonuclease, a guide-polynucleotide and a donor molecule for repair of the DNA break.Type: ApplicationFiled: September 9, 2017Publication date: July 25, 2019Inventors: Katelijn D'HALLUIN, Eveline BOSSIER
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Publication number: 20190225975Abstract: The present technology provides trichome specific promoters of cannabinoid biosynthesis enzyme genes from Cannabis, nucleotide sequences of the trichome specific promoters, and uses of the promoters for modulating the production of cannabinoids and other compounds in organisms. The present technology also provides chimeric genes, vectors, and transgenic cells and organisms, including plant cells and plants, comprising the trichome specific promoters. Also provided are methods for expressing nucleic acid sequences in cells and organisms using the trichome specific promoters.Type: ApplicationFiled: September 14, 2017Publication date: July 25, 2019Applicant: 22nd Century Limited, LLCInventor: Paul Rushton
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Publication number: 20190225976Abstract: The present invention relates to the field of plant genetic engineering, particularly the modulation of gene expression in plant cells for improved regeneration competence. The invention discloses means and methods wherein the Ethylene Response Factor (ERF) and Phytochrome A Signal Transduction 1 (PAT1) transcription factor complex activity in plant cells grants increased regeneration.Type: ApplicationFiled: October 11, 2017Publication date: July 25, 2019Inventors: Lieven De Veylder, Jefri Heyman
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Publication number: 20190225977Abstract: Pennycress (Thlaspi arvense) seed, seed lots, seed meal, and compositions with reduced glucosinolate content as well as plants that yield such seed, seed lots, seed meal, and compositions are provided. Methods of making and using the pennycress plants and/or seed that provide such seed, seed lots, seed meal, and compositions are also provided.Type: ApplicationFiled: January 18, 2019Publication date: July 25, 2019Inventors: Tim Ulmasov, John C. Sedbrook, Michael David Marks, Michaela G. McGinn, Cristine Handel, Ratan Chopra, Brice Allen Jarvis
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Publication number: 20190225978Abstract: A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a GDP-4-dehydro-6-deoxy-D-mannose reductase (RMD) the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.Type: ApplicationFiled: September 29, 2017Publication date: July 25, 2019Inventors: MARC-ANDRE D'AOUST, PIERRE-OLIVIER LAVOIE, ANDEOL FALCON DE LONGEVIALLE
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Publication number: 20190225979Abstract: Provided is an influenza mucosal vaccine composition and preparation and application thereof. This composition contains an antigen fusion protein which includes an influenza virus antigen and a Type IIb heat-labile enterotoxin A subunit from Escherichia coli. Immunization with this antigen fusion protein induces cellular and humoral immune responses, including systemic and mucosal immune responses, against a specific influenza virus in a subject, and therefore protects the subject from viral infection.Type: ApplicationFiled: August 3, 2018Publication date: July 25, 2019Inventors: Suh-Chin Wu, Shi-Wei Lin, Neos Tang, Ting-Hsung Chen
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Publication number: 20190225980Abstract: The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline and/or oxidative stress conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline and/or oxidative stress conditions with respect to wild-type plants grown under similar conditions.Type: ApplicationFiled: February 1, 2019Publication date: July 25, 2019Inventors: Fasong Zhou, Kenneth A. Feldmann, Julissa Sosa
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Publication number: 20190225981Abstract: The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline conditions with respect to wild-type plants grown under similar conditions.Type: ApplicationFiled: February 14, 2019Publication date: July 25, 2019Inventors: Fasong Zhou, Kenneth A. Feldmann, Julissa Sosa
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Publication number: 20190225982Abstract: The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline conditions with respect to wild-type plants grown under similar conditions.Type: ApplicationFiled: February 14, 2019Publication date: July 25, 2019Inventors: Fasong Zhou, Kenneth A. Feldmann, Julissa Sosa
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Publication number: 20190225983Abstract: The application concerns melon plants (Cucumis melo) resistant to infection with tomato leaf curl New Dehli virus (ToLCNDV). The resistant melon plants have a genomic introgression fragment on chromosome 5 which confers tolerance to ToLCNDV in a dominant manner. Also disclosed are markers for identifying those fragments, methods for identifying or producing resistant melon plants.Type: ApplicationFiled: July 7, 2017Publication date: July 25, 2019Applicant: NUNHEMS B.V.Inventors: Juan Antonio LIZARZABURU CHAVEZ, Jeffrey SKONECZKA, Daniel BELLON DOÑA
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Publication number: 20190225984Abstract: The present invention relates to a Cucumis melo plant which carries QTL1 in its genome that leads to resistance against the Bemisia tabaci species complex, which QTL1 is located between flanking marker sequences SEQ ID No. 1 and SEQ ID No. 2 and can be identified by and is in particular linked to one or more markers selected from the group consisting of SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, and SEQ ID No. 12, or combinations thereof. In a further embodiment, the Cucumis melo plant further comprises another Bemisia resistance conferring QTL, which combination of QTLs leads to an improved level of resistance to the Bemisia tabaci species complex when compared to a plant in which only the other QTL is present.Type: ApplicationFiled: March 6, 2019Publication date: July 25, 2019Inventors: Lena Johanna Huijbregts-Doorduin, Magdalena Barbara Lastdrager, Albertus Cornelius Maria Van Den Ende, Johanna Evertje Van Elven, Ram Kumar Basnet
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Publication number: 20190225985Abstract: Disclosed herein are transgenic plants comprising heterologous molecular chaperone genes and insecticidal Bacillus thuringiensis (Bt) genes capable of conferring enhanced Bt insecticidal activity to insects. The disclosure further relates to methods of producing transgenic plants, enhancing efficacy of a Bt toxin and controlling insect populations in areas of cultivation using transgenic plants co-expressing a heterologous molecular chaperone gene and a Bt gene.Type: ApplicationFiled: September 8, 2017Publication date: July 25, 2019Inventors: Maria Alejandra Bravo de la Parra, Blanca Ines Garcia Gomez, Mario Soberón Chavez
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Publication number: 20190225986Abstract: The present inventors successfully introduced genes into stem cells of airway epithelial tissues using simian immunodeficiency virus vectors pseudotyped with F and HN, which are envelope glycoproteins of Sendai virus. Gene transfer into airway epithelial tissue stem cells using a vector of the present invention is useful for gene therapy of genetic respiratory diseases such as cystic fibrosis. Furthermore, it is possible to select respiratory organs such as the lungs as production tissues for providing proteins that are deficient due to genetic diseases.Type: ApplicationFiled: November 30, 2018Publication date: July 25, 2019Inventors: Katsuyuki Mitomo, Makoto Inoue, Hitoshi Iwasaki, Mamoru Hasegawa, Eric W. Alton, Uta Griesenbach
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Publication number: 20190225987Abstract: The invention provides a bidirectional hCMV-rhCMV promoter and recombinant vectors and recombinant virus comprising the bidirectional hCMV-rhCMV promoter operably linked to a first transgene in one direction and to a second transgene in the opposite direction. The invention also provides methods of making and using such recombinant vectors and recombinant virus.Type: ApplicationFiled: June 19, 2017Publication date: July 25, 2019Inventors: Kerstin WUNDERLICH, Jort VELLINGA
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Publication number: 20190225988Abstract: The instant invention relates to methods for the in vivo assembly and integration of a polynucleotide of interest at a specific chromosomal target site in a fungal host cell, said method comprising transforming the host cell with: iv. at least one polynucleotide fragment comprising an upstream flanking region and a 5? part of the polynucleotide of interest in that order; v. at least one polynucleotide fragment comprising a 3? part of the polynucleotide of interest and a downstream flanking region in that order; and, optionally vi.Type: ApplicationFiled: September 13, 2017Publication date: July 25, 2019Applicant: Novozymes A/SInventor: Dorte M. K. Klitgaard
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Publication number: 20190225989Abstract: A gene knockin method and a kit for gene knockin are provided.Type: ApplicationFiled: January 19, 2018Publication date: July 25, 2019Applicant: Institute of Hematology and Blood Disease Hospital, CAMS & PUMCInventors: Tao CHENG, Jianping ZHANG, Xiaolan LI, Xiao-Bing ZHANG
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Publication number: 20190225990Abstract: Methods of editing a target genomic region(s), methods of repairing of a DNA break via a HDR pathway, methods of inhibiting or suppressing repair of a DNA break via a NHEJ pathway, and methods of modifying expression of a gene(s) or protein(s) comprise administering to one or more cells that include one or more target genomic regions, a genome editing system and a DNA protein-kinase (DNAPK) inhibitor disclosed herein. Kits and compositions for editing a target gene comprise a genome editing system and a DNAPK inhibitor disclosed herein.Type: ApplicationFiled: July 13, 2017Publication date: July 25, 2019Applicant: Vertex Pharmaceuticals IncorporatedInventors: Norzehan Abdul-Manan, David A. Newsome, Jacque Zwahlen
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Publication number: 20190225991Abstract: Disclosed herein are homology-independent targeted integration methods of integrating an exogenous DNA sequence into a genome of a non-dividing cell and compositions for such methods. Methods herein comprise contacting the non-dividing cell with a composition comprising a targeting construct comprising the exogenous DNA sequence and a targeting sequence, a complementary strand oligonucleotide homologous to the targeting sequence, and a nuclease, thereby altering the genome of the non-dividing cell.Type: ApplicationFiled: July 14, 2017Publication date: July 25, 2019Inventors: Juan Carlos IZPISUA BELMONTE, Keiichiro SUZUKI, Reyna HERNANDEZ-BENITEZ, Jun WU, Yuji TSUNEKAWA
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Publication number: 20190225992Abstract: Methods and compositions are provided for modifying one or more target loci in a cell. Such methods comprise providing a cell comprising a first polynucleotide encoding a first selection marker operably linked to a first promoter active in the cell, wherein the first polynucleotide further comprises a first recognition site for a first nuclease agent. A first nuclease agent is introduced into a cell, wherein the first nuclease agent induces a nick or double-strand break at the first recognition site. Further introduced into the cell is a first targeting vector comprising a first insert polynucleotide flanked by a first and a second homology arm that correspond to a first and a second target site located in sufficient proximity to the first recognition site. At least one cell is then identified comprising in its genome the first insert polynucleotide integrated at the target locus.Type: ApplicationFiled: March 29, 2019Publication date: July 25, 2019Applicant: Regeneron Pharmaceuticals, Inc.Inventors: Wojtek Auerbach, David Frendewey, Gustavo Droguett, Anthony Gagliardi, Junko Kuno, David M. Valenzuela
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Publication number: 20190225993Abstract: Methods to produce microbial biomass are provided. The methods involve the inoculation of a liquid growth medium including a controlled substrate with a microbial inoculum and incubation under conditions that are suitable for the production of biomass. The biomass and/or the liquid growth medium may be used to produce valuable products including food and feed products.Type: ApplicationFiled: January 22, 2019Publication date: July 25, 2019Inventors: Weiwei ZHAO, Jianhua SONG, Xuefeng WANG, Qingye JIANG
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Publication number: 20190225994Abstract: The invention relates to a two phase fermentation process for producing an organic compound, in particular an isoprenoid and to a bioreactor comprising a two phase fermentation system for producing an organic compound.Type: ApplicationFiled: January 25, 2019Publication date: July 25, 2019Inventors: Antonius Cornelis Johannes Matheus JANSSEN, Joannes Gerardus Theodorus KIERKELS, Georg Friedrich LENTZEN
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Publication number: 20190225995Abstract: Process are provided which are effective for controlling medium conductivity during fermentation of a CO-containing gaseous substrate while providing an STY of about 10 g ethanol/(L·day) or more. The process includes balancing medium conductivity, specific carbon uptake or cell density levels.Type: ApplicationFiled: November 14, 2017Publication date: July 25, 2019Inventors: Ryan H. Senaratne, Peter Simpson Bell, Song Liu, Syrona R. Scott
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Publication number: 20190225996Abstract: Disclosed are methods and compositions for increasing the triacylglycerol content of a cell by increasing the activity of a type 1 diacylglycerol acyltransferase (i.e., DGA2) and increasing the activity of a type 2 diacylglycerol acyltransferase (i.e., DGA1). In some embodiments, the triacylglycerol content of a cell is also modified my decreasing the activity of a triacylglycerol lipase in the same cell. Also disclosed are methods and compositions for increasing the triacylglycerol content of a cell by increasing the activity of a type 1 diacylglycerol acyltransferase (i.e., DGA2), or by increasing the activity of a type 3 diacylglycerol acyltransferase (i.e., DGA3).Type: ApplicationFiled: April 5, 2019Publication date: July 25, 2019Inventors: Vasiliki TSAKRAKLIDES, Elena E. BREVNOVA
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Publication number: 20190225997Abstract: The present disclosure relates to fructose-6-phosphate-3-epimerase consisting of an amino acid sequence of SEQ ID NO: 1, a nucleic acid encoding the fructose-6-phosphate-3-epimerase, and a transformant comprising the nucleic acid. Additionally, the present disclosure relates to a composition for producing allulose, which comprises the fructose-6-phosphate-3-epimerase of the present disclosure, and a method for producing allulose using the fructose-6-phosphate-3-epimerase of the present disclosure.Type: ApplicationFiled: June 30, 2017Publication date: July 25, 2019Inventors: Sungjae YANG, Hyun Kug CHO, Young Mi LEE, Seong Bo KIM, Seong Jun CHO
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Publication number: 20190225998Abstract: The present disclosure relates to synthesis of heparin, which may be bioequivalent to porcine USP Heparin Sodium. The synthesis may involve three intermediates starting from heparosan.Type: ApplicationFiled: September 7, 2017Publication date: July 25, 2019Applicant: Rensselaer Polytechnic InstituteInventors: Marc DOUAISI, Navdeep GROVER, Payel DATTA, Elana PASKALEVA, Lei LIN, Paul BRODFUEHRER, Trevor J. SIMMONS, Akihiro ONISHI, Makoto HIRAKANE, Li FU, Kevin LI, Robert J. LINHARDT, Jonathan DORDICK, Daisuke MORI
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Publication number: 20190225999Abstract: This invention discloses multi-part primers for primer-dependent nucleic acid amplification methods. Also disclosed are multiplex assay methods, related reagent kits, and oligonucleotides for such methods.Type: ApplicationFiled: April 5, 2017Publication date: July 25, 2019Applicant: Rutgers, The State University of New JerseyInventors: Salvatore A. E. MARRAS, Diana VARGAS-GOLD, Sanjay TYAGI, Fred Russell KRAMER
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Publication number: 20190226000Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism of the genus Yarrowia in a suitable fermentation medium at a temperature of about 29° C. or higher, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol; and b. recovering the diterpene or glycosylated diterpene.Type: ApplicationFiled: March 12, 2019Publication date: July 25, 2019Inventors: Nicolette Jasmijn BROERS, Viktor Marius BOER, Adam G. LAWRENCE
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Publication number: 20190226001Abstract: Methods are provided to improve the expression of protein complexes by tuning the expression levels of each component required for the assembly of the complex. These methods are effective in limiting the expression of the dominant chain and, thus, equilibrating their relative abundance. The methods provided herein lead to a significant increase in productivity and final bispecific yields both in transient expression systems as well as in stably transfected mammalian cells.Type: ApplicationFiled: September 28, 2018Publication date: July 25, 2019Inventors: Giovanni MAGISTRELLI, Pauline MALINGE, Yves POITEVIN, Nicolas FISCHER
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OBESITY-RELATED DISEASE THERAPEUTIC AGENT BY HEPATIC SECRETORY METABOLIC REGULATOR INHIBITORY ACTION
Publication number: 20190226002Abstract: Disclosed are methods for screening a drug for treating obesity-related diseases by using the ability of candidate drugs to inhibit the function of pentraxin-4 as an index, and a pharmaceutical composition for treating obesity-related diseases comprising a pentraxin-4 inhibitor as an active ingredient. The drug screened by the above-mentioned method and the pharmaceutical composition are useful for treating obesity-related diseases, particularly for non-alcoholic steatohepatitis (NASH).Type: ApplicationFiled: May 12, 2017Publication date: July 25, 2019Applicant: The University of TokyoInventors: Yoshihiko IZUMIDA, Nobuhiro WADA, Yukari MASUDA, Takashi KADOWAKI -
Publication number: 20190226003Abstract: A method of selecting an agent for treating a disease of a subject is disclosed which includes identifying genes which bring about resistance to a cytotoxic agent in haploid human embryonic stem (ES) cells. Once the gene is identified, the method includes analyzing the sequence and/or expression of the gene in a cell sample of the subject, wherein an alteration in the sequence and/or level of expression of the gene as compared to the sequence and/or expression of the gene in a control sample is indicative that the agent should be ruled out as a monotherapy for treating the disease in the subject.Type: ApplicationFiled: June 19, 2016Publication date: July 25, 2019Inventor: Nissim BENVENISTY
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Publication number: 20190226004Abstract: The present invention provides systems and methods for surveillance, diagnosis, and evaluation of high risk bacterial transmission events. The systems and methods utilize software and computational systems that automate identification, surveillance, and communication. The invention further includes archival systems for use in the systems and methods that compile bacterial isolates linked to information about patients, pre-operative, intra-operative, or post-operative arenas, healthcare providers, and the like.Type: ApplicationFiled: January 25, 2019Publication date: July 25, 2019Inventor: Randy W. Loftus
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Publication number: 20190226005Abstract: Multidifferential agar for differentially detecting Bile-Tolerant Gram-Negative organisms, E. coli, Salmonella, and non-E. coli coliforms and methods for using the same are disclosed. The multidifferential agar may include chromogenic substrate(s), agar, gelatin, an inhibitor of gram-positive bacteria, bacterial nutrients, propylene glycol, surfactants, and/or bile salts.Type: ApplicationFiled: January 19, 2018Publication date: July 25, 2019Inventors: Molly Paridon, Sommer Teague, Stephanie Bernards
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Publication number: 20190226006Abstract: Methods and compositions are provided for detecting and/or determining a patient's risk of developing hyperglycemia or type 1 diabetes. The methods are directed to analyzing the miRNA content of extracellular vesicles recovered from said patient.Type: ApplicationFiled: January 17, 2019Publication date: July 25, 2019Inventors: Raghavendra G. Mirmira, Carmella Evans-Molina, Emily K. Sims
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Publication number: 20190226007Abstract: The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps: a. binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase; wherein the sample makes up at least 85% of the volume of the binding mixture; b. separating the solid phase with the bound extracellular nucleic acids; c. optionally washing the extracellular nucleic acids; d. optionally eluting extracellular nucleic acids from the solid phase. The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.Type: ApplicationFiled: November 29, 2018Publication date: July 25, 2019Inventors: Martin Horlitz, Annette Nocon, Markus Sprenger-Haussels, Peter Grünefeld, Christoph Erbacher
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Publication number: 20190226008Abstract: Methods and reagent for determining the presence and/or for quantifying the amount of a target nucleic acid sequences in a sample are provided. In some aspects, the methods comprise performing a melt analysis by detecting, a signal from a probe at a temperature that is lower than the Tm of the probe and a signal at a temperature that is higher than the Tm of the probe, without detecting a signal at the Tm of the probe.Type: ApplicationFiled: January 22, 2019Publication date: July 25, 2019Applicant: LUMINEX CORPORATIONInventors: Douglas WHITMAN, Jennifer BERNIER, William WANG
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Publication number: 20190226009Abstract: A method of identifying a subject having a bacterial infection, which includes discriminating said subject from a subject having a viral infection or an inflammatory disease. Also provided is a gene signature employed in the method and to a bespoke gene chip for use in the method. Further provided are probes and/or primers specific to genes in a signature of the present disclosure.Type: ApplicationFiled: July 12, 2017Publication date: July 25, 2019Applicant: Imperial Innovations Ltd.Inventors: Michael Levin, Myrsini Kaforou, Jethro A. Herberg, Victoria J. Wright, Lachlan J.M. Coin
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Publication number: 20190226010Abstract: A sample handling system and a process for the handling of chemical or biological samples is provided. The system includes a dosing device with a receiving plate configured in an essentially horizontal position in a plane defined by a first and a second axis. The dosing device has at least one working arm that is movable. The pipetting mechanism includes at least one pipetting channel that is movable and has a pipette tip mounted or mountable thereon. The system further includes at least one thermal cycler provided in the area of the receiving plate. The thermal cycler is configured on the receiving plate in such a way that the pipetting mechanism is movable in a position above the sample vessels in the thermal cycler, in a setting that allows a pipetting step to be carried out into the sample vessels in the thermal cycler by the pipetting mechanism in the position above the sample vessels in the thermal cycler.Type: ApplicationFiled: April 2, 2019Publication date: July 25, 2019Applicant: HAMILTON Bonaduz AGInventors: Martin GAJEWSKI, Mario ARANGIO
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Publication number: 20190226011Abstract: The present invention relates to a method for the in vitro detection of a latent tuberculosis infection (LTBI) in a subject, wherein said method comprises determining at least one nucleotide sequence and/or at least one polypeptide of Mycobacterium tuberculosis (Mtb) in a blood cell population of said subject, and wherein the presence of said at least one nucleotide sequence and/or said at least one polypeptide is indicative for said latent tuberculosis infection. In particular, the blood cell population is enriched for hematopoietic stem cells. The invention also relates to a pharmaceutical composition for use in the treatment of the LTBI in the subject, wherein it is determined if the nucleotide sequence and/or the polypeptide of Mtb is/are present in the blood cell population. Further, the invention relates to kits for carrying out the methods of the invention. The invention also relates to the use of the kits.Type: ApplicationFiled: June 6, 2017Publication date: July 25, 2019Applicants: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., Medizinische Universität WienInventors: Julia Tornack, Steephen REECE, Fritz MELCHERS, Stefan H.E. KAUFMANN, Wolfgang BAUER, Georg STINGL
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Publication number: 20190226012Abstract: Provided are methods and systems for detecting formation of nucleotide-specific ternary complexes comprising a DNA polymerase, a nucleic acid, and a nucleotide complementary to the templated base of the primed template nucleic acid. The methods and systems facilitate determination of the next correct nucleotide without requiring chemical incorporation of the nucleotide into the primer. These results can even be achieved in procedures employing unlabeled, native nucleotides.Type: ApplicationFiled: April 2, 2019Publication date: July 25, 2019Applicant: Omniome, Inc.Inventors: Pinar Iyidogan, Kandaswamy Vijayan
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Publication number: 20190226013Abstract: Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.Type: ApplicationFiled: April 8, 2019Publication date: July 25, 2019Applicant: HTG Molecular Diagnostics, Inc.Inventors: Bruce A. Seligmann, BJ Kerns, John Luecke, Matt Rounseville, Ihab Botros, Mark Schwartz
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Publication number: 20190226014Abstract: A method of sequencing a nucleic acid such as DNA or RNA comprising (1) generating a stream of single nucleoside triphosphates; (2) producing at least one substantially double-stranded oligonucleotide used probe by reacting the single nucleoside triphosphates with a corresponding biological probe comprising (a) a first single-stranded oligonucleotide including a restriction endonuclease nicking-site, a single nucleotide capture site and oligonucleotide regions juxtaposed either side of the nicking-site having at least one fluorophore and at least one quencher and (b) second and third single-stranded oligonucleotides; (3) nicking the first oligonucleotide strand of the used probe at the nicking-site; (4) separating the first oligonucleotide components generated in step (3) from the complementary strand of the used probe and (5) detecting the fluorophores. New biological probes and probe systems for use with the method are also described.Type: ApplicationFiled: September 4, 2017Publication date: July 25, 2019Applicant: Base4 Innovation LimitedInventors: Barnaby BALMFORTH, Cameron Alexander FRAYLING
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Publication number: 20190226015Abstract: Disclosed is a method of performing a non-isothermal nucleic acid amplification reaction, the method comprising the steps of: (a) mixing a target sequence with one or more complementary single stranded primers in conditions which permit a hybridisation event in which the primers hybridise to the target, which hybridisation event, directly or indirectly, leads to the formation of a duplex structure comprising two nicking sites disposed at or near opposite ends of the duplex; and performing an amplification process by; (b) causing a nick at each of said nicking sites in the strands of the duplex; (c) using a polymerase to extend the nicked strands so as to form newly synthesised nucleic acid, which extension with the polymerase recreates nicking sites; (d) repeating steps (b) and (c) as desired so as to cause the production of multiple copies of the newly synthesised nucleic acid; characterised in that the temperature at which the method is performed is non-isothermal, and subject to a reduction of at least 2°Type: ApplicationFiled: June 30, 2017Publication date: July 25, 2019Applicant: LUMIRADX UK LTDInventors: Jarrod PROVINS, Daiwei SHEN, Bryan KRAYNACK
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Publication number: 20190226016Abstract: Methods and compositions are provided for detecting and/or determining a patient's risk of developing hyperglycemia or type 1 diabetes. The methods are directed to analyzing the miRNA and protein content of extracellular vesicles recovered from a patient.Type: ApplicationFiled: March 4, 2019Publication date: July 25, 2019Inventors: Raghavendra G. MIRMIRA, Carmella EVANS-MOLINA, Emily K. SIMS
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Publication number: 20190226017Abstract: Disclosed herein are methods and compositions such as primers, primer pairs, and kits for typing KIR2DL1, KIR2DL2, and KIR2DL3 alleles. The compositions and methods disclosed herein are useful in the selection of most appropriate donors for HCT.Type: ApplicationFiled: September 28, 2017Publication date: July 25, 2019Applicant: MEMORIAL SLOAN KETTERING CANCER CENTERInventors: Katharine HSU, Jean-Benoit LE LUDUEC
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Publication number: 20190226018Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: ApplicationFiled: January 24, 2018Publication date: July 25, 2019Inventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan