Patents Issued in November 28, 2019
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Publication number: 20190360015Abstract: The present invention relates to a cell for the production of an antibody molecule such as an antibody useful for various diseases having high antibody-dependent cell-mediated cytotoxic activity, a fragment of the antibody and a fusion protein having the Fc region of the antibody or the like, a method for producing an antibody composition using the cell, the antibody composition and use thereof.Type: ApplicationFiled: January 29, 2019Publication date: November 28, 2019Applicant: KYOWA HAKKO KIRIN CO., LTDInventors: Yutaka Kanda, Mitsuo Satoh, Kazuyasu Nakamura, Kazuhisa Uchida, Toyohide Shinkawa, Naoko Yamane, Emi Hosaka, Kazuya Yamano, Motoo Yamasaki, Nobuo Hanai
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Publication number: 20190360016Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.Type: ApplicationFiled: August 9, 2019Publication date: November 28, 2019Applicant: AMGEN INC.Inventors: Chung-Jr HUANG, Xiaoming YANG
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Publication number: 20190360017Abstract: The present disclosure relates generally to an improved gene expression system in the field of recombinant gene expression. The invention relates to a system showing an improved yield and quality of protein production and methods for increasing production of a protein produced by cultured cells, particularly cultured eukaryotic cells.Type: ApplicationFiled: December 22, 2017Publication date: November 28, 2019Inventors: Kim De Keersmaecker, Sergey O. Sulima, Tiziana Girardi, Kim R Kampen
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Publication number: 20190360018Abstract: A method for quantifying a target substance, comprising: bringing a sample containing the target substance into contact with a biosensor which comprises an enzyme electrode containing an oxidoreductase and a counter electrode; measuring a change in the potential difference between the enzyme electrode and the counter electrode due to oxidation reaction of the target substance catalyzed by the oxidoreductase; and calculating the concentration of the target substance based on the change in the potential difference; wherein a potential is applied between the enzyme electrode and the counter electrode before the measurement of the change in the potential difference.Type: ApplicationFiled: May 21, 2019Publication date: November 28, 2019Applicants: ARKRAY, Inc., Ultizyme International Ltd.Inventors: Koji Sode, Inyoung Lee, Wakako Tsugawa
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Publication number: 20190360019Abstract: Disclosed herein are methods and products for the incorporation of an enzyme mediated reaction step and/or cascading amplification methods that are activated via a molecule cleavage event and methods and products for cleavage detection in assays where a binding event can be used to selectively detect the analyte of interest.Type: ApplicationFiled: November 16, 2017Publication date: November 28, 2019Inventors: Alastair M. HODGES, Ronald CHATELIER
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Publication number: 20190360020Abstract: An oil-phase composition for generating water-in-oil droplets by means of centrifugation, consisting of the following components: 7-15% (v/v) of a long-chain alkyl-containing silicon-oxygen chain nonionic surfactant and 0-10% of mineral oil, with the balance being diethylhexyl carbonate; or consisting of the following components: 85-95% (v/v) of a long-chain alkane ester and 5-15% (v/v) of a long-chain alkyl-containing silicon-oxygen chain nonionic surfactant; or consisting of silicone oil and a surfactant. Also provided is a method for generating water-in-oil droplets by means of centrifugation using the oil-phase composition as a second liquid.Type: ApplicationFiled: May 25, 2017Publication date: November 28, 2019Applicant: Geekgene Biotechnology, Inc.Inventors: Yanyi HUANG, Zitian CHEN, Fangli ZHANG, Peiyu LIAO
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Publication number: 20190360021Abstract: Provided are a composition for a polymerase reaction, containing a nucleic acid polymerase and a 2-methacryloyloxyethyl phosphorylcholine (MPC)-containing zwitterionic copolymer detergent, a tube for a polymerase reaction, and a kit for a polymerase reaction. The stability of the composition for a polymerase reaction can be improved and the reliability of the results of polymerase reaction such as nucleic acid polymerization or amplification can be improved.Type: ApplicationFiled: November 21, 2017Publication date: November 28, 2019Inventors: Minsu KO, Youngmi LEE, Kayoung LEE, Junsang KO
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Publication number: 20190360022Abstract: The present invention relates to a biomarker of predicting or diagnosing female genital disease or obesity. More specifically, the present invention relates to a composition for predicting or diagnosing risk of female genital disease or obesity, including detecting Prevotella spp., Sneathia spp. Megasphaera spp., Gardnerella spp., and Lactobacillus spp.Type: ApplicationFiled: September 18, 2017Publication date: November 28, 2019Inventors: GwangPyo KO, Jiyeon SI, Hyun Ju YOU
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Publication number: 20190360023Abstract: The present invention, SMASH (Short Multiply Aggregated Sequence Homologies), is a technique designed to pack multiple independent mappings into every read. Specifically, the invention relates to a composition comprising a first mixture of different chimeric genomic nucleic acid fragments, wherein each different fragment in the mixture comprises randomly ligated DNA segments, wherein each DNA segment in the fragment is a nucleic acid molecule at least 27 base pairs in length resulting from random fragmentation of a single genome. The invention also relates to methods for generating said composition and use of said composition to obtain genomic information, for example, copy number variation.Type: ApplicationFiled: May 3, 2019Publication date: November 28, 2019Applicant: Cold Spring Harbor LaboratoryInventors: Michael H. Wigler, Dan Levy, Zihua Wang
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Publication number: 20190360024Abstract: Provided herein are kits, compositions, and methods for diagnosing and treating interstitial cystitis (IC) and/or interstitial cystitis/bladder pain syndrome (IC/BPS) based on finding lower levels of certain bacteria in a subject's stool sample (e.g., O. splanchnicus, F. prausnitzii, C. aerofaciens, E. sinensis, L. longoviformis, and R. intestinalis). In certain embodiments, then present invention provides probiotic formulations containing live bacteria (e.g., from O. splanchnicus, F. prausnitzii, C. aerofaciens, E. sinensis, L. longoviformis, and R. intestinalis).Type: ApplicationFiled: June 10, 2019Publication date: November 28, 2019Inventors: David J. Klumpp, Anthony J. Schaeffer, Bryan A. White
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Publication number: 20190360025Abstract: Provided herein is a circular proximity ligation assay in which proximity-probes are employed as bridges to connect two free oligonucleotides via a dual ligation event, resulting in the formation of a circle. The circles are then quantified by, e.g., qPCR. The addition of an extra oligonucleotide is believed to enhance specificity by decreasing the probability of random background ligation events. In addition, circle formation may have selective advantages, as uncircularized DNA can be removed by a simple exonuclease treatment and it has streamlined the workflow by eliminating preamplification prior to qPCR.Type: ApplicationFiled: February 20, 2018Publication date: November 28, 2019Inventors: Henrik H.J. PERSSON, Roxana JALILI, Joseph L. HORECKA, Ronald W. DAVIS
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Publication number: 20190360026Abstract: The present invention relates to a nanoplasmonic biosensor capable of label-free multiplex detection of disease markers in blood with high selectivity and sensitivity and a method for detecting disease markers using the nanoplasmonic biosensor. The nanoplasmonic biosensor of the present invention enables label-free multiplex detection of miRNAs as disease markers in blood with high selectivity and sensitivity. Therefore, the nanoplasmonic biosensor of the present invention can be effectively used for the diagnosis of miRNA-related diseases and clinical applications.Type: ApplicationFiled: March 22, 2019Publication date: November 28, 2019Applicant: Korea University Research and Business FoundationInventors: Sang Jun SIM, Jong-Uk LEE, Woo-hyun KIM
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Publication number: 20190360027Abstract: Disclosed herein are methods and compositions for associating a genetic variant with intraretinal fluid. Also disclosed herein are methods and compositions for associating a genetic variant with visual acuity, anatomic outcomes or treatment frequency.Type: ApplicationFiled: May 25, 2018Publication date: November 28, 2019Inventors: Lorah Perlee, Sara Hamon
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Publication number: 20190360028Abstract: A method of distinguishing between lung squamous cell carcinoma and head and neck squamous cell carcinoma using a 22-gene biomarker signature is presented.Type: ApplicationFiled: August 7, 2019Publication date: November 28, 2019Inventors: Anthony M. Magliocco, Soner Altiok, Farah K. Khalil, Yin Xiong
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Publication number: 20190360029Abstract: Provided are methods of targeting patient-specific oncogenes in extrachromosomal DNA (ecDNA) to treat glioma in a human. The present methods include identifying a drug that targets against an oncogene present in ecDNA of a human suffering from glioma, such as glioblastoma. The identified oncogenes present in ecDNA include MET, MET/CAPZA2, MDM2, CDK4, SOX2, PIK3CA, MECOM, PDGFRA, EGFR, MYCN, MYC, TERT, SMARCA4, RP56, FBXW7, CDK6, CCND2, ERBB2, BRCA1, and BAP1. The present methods include identifying a drug targeted against the ecDNA oncogene, which drug inhibits the function of the identified oncogene, so as to inhibit tumor growth or progression of the glioma in the human. Also provided are PDX mouse models to further identify and/or confirm patient-specific drugs that target the identified oncogene(s) present in ecDNA. Also provided are methods of diagnosing gliomas or recurrent gliomas and methods of screening or monitoring for recurrence of gliomas.Type: ApplicationFiled: January 19, 2018Publication date: November 28, 2019Inventors: Roel VERHAAK, Hoon KIM, Ana DECARVALHO, Tom MIKKELSEN
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Publication number: 20190360030Abstract: This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes.Type: ApplicationFiled: June 14, 2019Publication date: November 28, 2019Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes, Derek L. Stemple
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Publication number: 20190360031Abstract: A method of amplifying a target nucleic acid (polynucleotide) contained in a particle including an enclosing lipid bilayer membrane according to the present invention includes the steps of: lysing the particle which is stored in a compartment constituted by a liquid in an amount of 100 ?l or less; and amplifying the target nucleic acid in the compartment.Type: ApplicationFiled: May 23, 2019Publication date: November 28, 2019Inventors: Toru Uchiyama, Masafumi Onodera
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Publication number: 20190360032Abstract: Provided are methods of depleting a target nucleic acid in a sample. The methods include contacting a target nucleic acid with two or more polymers that specifically hybridize to the target nucleic acid, and cleaving the hybridized regions of the target nucleic acid to deplete the target nucleic acid in the sample. Kits for practicing the subject methods are also provided.Type: ApplicationFiled: August 9, 2019Publication date: November 28, 2019Inventors: Andrew Alan Farmer, Ian B. Oglesby, Magnolia Bostick, Steve Oh
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Publication number: 20190360033Abstract: This invention relates to methods and compositions for assessing an amount of non-native nucleic acids in a sample, such as from a subject. The methods and compositions provided herein can be used to determine risk of a condition, such as transplant rejection, in subject.Type: ApplicationFiled: November 2, 2017Publication date: November 28, 2019Applicant: The Medical College of Wisconsin, Inc.Inventors: Karl Stamm, Aoy Tomita Mitchell, Michael Mitchell
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Publication number: 20190360034Abstract: The present invention provides methods and systems for sequencing long nucleic acid fragments. In one aspect of the invention, methods, systems and reagent kits are provided for sequencing nucleic acid target sequences. Some embodiments of the methods, systems and reagent kits are particularly suitable for sequencing a large number of fragments, particularly long fragments. Some embodiments of the methods, systems and reagent kits are for native extension parallel sequencing of polynucleotide.Type: ApplicationFiled: April 22, 2019Publication date: November 28, 2019Inventors: Wei Zhou, Rui Mei, Guochun Liao, Julian Lucas
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Publication number: 20190360035Abstract: Various methods, systems and devices for optical detection and analysis of polymers, such as polynucleotides, using nanopores, e.g., for determining sequences of nucleic acids, are provided herein. In certain variations, methods and systems for determining a nucleotide sequence of a polynucleotide, which include measuring mixed FRET signals as a polynucleotide translocates through a nanopore and determining a nucleotide sequence of the polynucleotide from the mixed FRET signals, are provided.Type: ApplicationFiled: May 24, 2019Publication date: November 28, 2019Applicant: Quantapore, Inc.Inventors: Martin HUBER, Bason E. CLANCY, Paul HARDENBOL
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Publication number: 20190360036Abstract: The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.Type: ApplicationFiled: May 28, 2019Publication date: November 28, 2019Applicant: Natera, Inc.Inventors: Matthew RABINOWITZ, George GEMELOS, Milena BANJEVIC, Allison RYAN, Zachary DEMKO, Matthew HILL, Bernhard ZIMMERMANN, Johan BANER
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Publication number: 20190360037Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived, and the nucleic acids sequences are designed such that the nucleic acid sequences produce a predefined pattern of detectable signals during a sequencing run. A method of preparing a quality control for performing nucleic acid sample sequencing, a method of validating a nucleic acid sequencing instrument during a nucleic acid sequencing experiment, and a method of processing nucleic acid sequencing data during a nucleic acid sequencing experiment are also disclosed.Type: ApplicationFiled: June 11, 2019Publication date: November 28, 2019Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Min-Yi SHEN, Douglas GREINER
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Publication number: 20190360038Abstract: Provided herein are methods and systems for sequencing a single nucleic acid molecule utilizing a polymerase enzyme, a template nucleic acid, and a polymerase reagent solution.Type: ApplicationFiled: December 15, 2017Publication date: November 28, 2019Inventor: Inanc ORTAC
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Publication number: 20190360039Abstract: A method and a device for determining a nucleotide sequence are proposed. The method comprises immobilising looped fragments of a nucleic acid and a polymerase on a sensor surface and adding a mixture of unlabelled nucleotides onto the sensor surface. Moreover, in the mixture added, one nucleotide type is present at a much lower concentration compared to the other nucleotides. Time intervals between each of the charge separation events are determined and the mixture addition and the registration steps are repeated. Moreover, at each repetition, the nucleotide type present at a much lower concentration compared to the other nucleotides in the mixture added is changed. The nucleotide sequence of a nucleic acid molecule is determined by the analysis of the time intervals between each of the charge separation events registered, which result from the insertion, facilitated by the polymerase, of said unlabelled nucleotides into the growing nucleic acid chain.Type: ApplicationFiled: March 29, 2018Publication date: November 28, 2019Applicant: LIMITED LIABILITY COMPANY "GAMMA-DNA" [RU/RU]Inventors: VLADIMIR IVANOVICH BASHKIROV, ANTON VLADIMIROVICH GRIGORIEV, MIKHAIL ALEXANDROVICH GUTOROV, EDUARD ANATOLIEVICH ILICHEV, VLADIMIR VLADIMIROVICH KOLESOV, KONSTANTIN VALERIEVICH KRUTOVSKY, ALEXEY OLEGOVICH MANTUROV
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Publication number: 20190360040Abstract: Provided are compositions, including products of manufacture and kits, and methods, for predicting the successful construction of a genomic T-Cell Receptor (TCR) library from a tissue sample; or predicting the successful sequencing of a TCR sequence from a tissue sample, wherein optionally the tissue sample comprises or is derived from a biopsy or a Formalin-Fixation and Paraffin Embedding (FFPE) sample, and optionally the tissue sample is a human or an animal sample. In alternative embodiments, provided herein are assays for predicting successful library construction and sequencing of a TCR chain. In alternative embodiments, the T cell is a tumor infiltrating lymphocyte (TIL). The results of methods provided herein can generate a prediction as to the success of assays used for quantifying T-cells and sequencing T-cell receptors, including any commercially available assay (e.g., ImmunoSeq, Adaptive Biotechnologies, Seattle, Wash.) to effectively assess TCR quantity and clonality.Type: ApplicationFiled: February 20, 2018Publication date: November 28, 2019Inventors: Stephen B. GRUBER, Marilena MELAS, Kevin MCDONNELL
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Publication number: 20190360041Abstract: Embodiments provided herewith are directed to self-assembled methods of preparing a patterned surface for sequencing applications including, for example, a patterned flow cell or a patterned surface for digital fluidic devices. The methods utilize photolithography to create a patterned surface with a plurality of microscale or nanoscale contours, separated by hydrophobic interstitial regions, without the need of oxygen plasma treatment during the photolithography process. In addition, the methods avoid the use of any chemical or mechanical polishing steps after the deposition of a gel material to the contours.Type: ApplicationFiled: May 17, 2017Publication date: November 28, 2019Inventors: Yir-Shyuan Wu, Yan-You Lin, M. Shane Bowen, Cyril Delattre, Fabien Abeille, Tarun Khurana, Arnaud Rival, Poorya Sabounchi, Dajun Yuan, Maria Candelaria Rogert Bacigalupo
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Publication number: 20190360042Abstract: An analytical device including an optically opaque cladding, a sequencing layer including a substrate disposed below the cladding, and a waveguide assembly for receiving optical illumination and introducing illumination into the device. The illumination may be received from a top, a side edge, and a bottom of the device. The waveguide assembly may include a nanoscale aperture disposed in the substrate and extending through the cladding. The aperture defines a reaction cell for receiving a set of reactants. In various aspects, the device includes a sensor element and the illumination pathway is through the sensor element. Waveguides and illumination devices, such as plasmonic illumination devices, are also disclosed. Methods for forming and operating the devices are also disclosed.Type: ApplicationFiled: November 26, 2018Publication date: November 28, 2019Inventors: Adrian Fehr, Nathaniel Joseph McCAFFREY, Stephen TURNER
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Publication number: 20190360043Abstract: Disclosed are methods and compositions for enriching nucleic acid fragments from a sample that include one or more target region of interest. In certain aspects, a sample of double stranded nucleic acid fragments having a strand-linking adapter at one end and a non-strand-linking adapter at the other end are denatured and contacted with capture probes specific for a target sequence of interest. Capture probe-bound fragments are isolated from the sample, e.g., using a solid substrate specific for the binding moiety on the capture probes, and are renatured for downstream processing, thus maintaining the original double-stranded region. This enrichment process does not require amplification and as such maintains the nucleic acids in their native states. The disclosed enrichment process and compositions are suitable for analyzing nucleic acids that are fragmented and/or damaged, e.g., cell-free DNA such as circulating tumor DNA, as well as nucleic acids that are many kilobases in length.Type: ApplicationFiled: May 21, 2019Publication date: November 28, 2019Inventors: Thang Pham, Stephen Turner, Keith Bjornson, Jeremiah Hanes
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Publication number: 20190360044Abstract: Provided herein are methods for generating single-cell molecular analysis comprising a) delivering one or more proximity dependent probes to a cell population, wherein each proximity dependent probe comprises a target binding region configured to bind a target RNA and a primer binding site region; b) linking bound proximity dependent probes; c) isolating single cells from the cell population in separate individual discrete volumes, the individual discrete volumes further comprising a primer pair and amplification reagents, wherein the primer pair binds to the primer binding sites of the ligation dependent probes, and wherein at least one primer comprises a barcode sequence that uniquely identifies the individual discrete volume; d) amplifying the ligated probes using the primer pair, wherein the barcode is incorporated into each resulting amplicon; and e) quantifying target RNAs in each individual cell based at least in part on sequencing the resulting amplicons.Type: ApplicationFiled: May 24, 2019Publication date: November 28, 2019Inventors: Fei CHEN, Jesse ENGREITZ, Jamie MARSHALL, Vidya SUBRAMANIAN, Sam Rodriques
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Publication number: 20190360045Abstract: Provided herein are methods, compositions, and kits for targeted sequencing of polynucleotides with high accuracy and low amplification and sequencing errors and bias.Type: ApplicationFiled: June 17, 2019Publication date: November 28, 2019Inventors: William Donahue, Francois Vigneault
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Publication number: 20190360046Abstract: A method for detecting a SNP site on a SMA gene is disclosed, and includes steps of: (S10) performing a PCR for amplifying a nucleic a nucleic acid fragment containing a SNP site; (S20) performing a dephosphorylation reaction on the nucleic acid fragment; (S30) performing an extension reaction on the nucleic acid fragment, wherein the SNP site is identified by using an extension primer, a 3?-end of the extension primer is extended by a single nucleotide which is complementary to a base of the SNP site, and thus an extended extension primer is obtained; (S40) performing a purification reaction; and (S50) measuring a molecular weight of the extended extension primer, and determining a type of a base of the single nucleotide based upon the molecular weight, thereby determining whether deletion occurs to the SNP site.Type: ApplicationFiled: December 5, 2018Publication date: November 28, 2019Inventors: HUNG MING CHANG, CHIEN HSING LIN, SHU MING CHANG
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Publication number: 20190360047Abstract: The present invention relates to methods for the identification of genetic polymorphisms that may be associated with a risk for QT prolongation after treatment with iloperidone and related methods of administering iloperidone to patients with such polymorphisms.Type: ApplicationFiled: May 8, 2019Publication date: November 28, 2019Inventors: Curt Wolfgang, Mihael Polymeropoulos, Christian Lavedan, Simona Volpi
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Publication number: 20190360048Abstract: The present invention relates to methods and kits for treating cardiovascular disease.Type: ApplicationFiled: January 12, 2018Publication date: November 28, 2019Inventors: Kenneth S. KORNMAN, Lynn DOUCETTE-STAMM, Gordon W. DUFF
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Publication number: 20190360049Abstract: It is intended to provide a rapid, convenient, and highly accurate method for determining the prognosis of cancer. The present invention provides a method for determining a tissue having renal cell carcinoma, comprising: (1) subjecting sample DNA to ion exchange chromatography, wherein the sample DNA is obtained by treating target genomic DNA prepared from a renal tissue of a subject with bisulfite, followed by PCR amplification; (2) calculating a derivative value of a detection signal of the chromatography; and (3) determining the renal tissue as being a tissue having renal cell carcinoma having poor prognosis when the derivative value calculated in the step (2) has two or more maximums.Type: ApplicationFiled: September 2, 2016Publication date: November 28, 2019Applicants: NATIONAL CANCER CENTER, SEKISUI MEDICAL CO., LTD.Inventors: Yae KANAI, Eri ARAI, Yuriko NEMOTO, Takuya YOTANI
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Publication number: 20190360050Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.Type: ApplicationFiled: February 11, 2019Publication date: November 28, 2019Inventors: Russell Higuchi, Stacey Ellen Wallace, Edwin Wei-Lung Lai
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Publication number: 20190360051Abstract: The invention provides methods of administering immunotherapy that modulates an interaction between PD-i and its ligand, to a cancer patient, based on tumor-educated gene expression profiles obtained from anucleated cells. The invention further provides methods of typing a sample of a subject for the presence or absence of a cancer, based on tumor-educated gene expression profiles obtained from anucleated cells. The invention further provides a method for obtaining a biomarker panel for typing of a sample from a subject using particle swarm optimization-based algorithms.Type: ApplicationFiled: February 19, 2018Publication date: November 28, 2019Inventors: Thomas Würdinger, Myron Ghislain Best
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Publication number: 20190360052Abstract: Disclosed herein are methods and kits for identifying a subject as having breast cancer. Also provided herein are methods and kits for determining the prognosis of a subject having breast cancer and for determining the progression of breast cancer in a subject.Type: ApplicationFiled: July 6, 2017Publication date: November 28, 2019Inventors: Kang ZHANG, Rui HOU, Lianghong ZHENG
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Publication number: 20190360053Abstract: The present invention provides methods and compositions that are useful for assessing gene expression for tumor immune response profile of a sample. In particular, a target-specific primer panel is provided that allows for selective amplification of immune response target sequences in a sample. In one aspect, the invention relates to target-specific primers useful for selective amplification of one or more target sequences associated with immune response. In some aspects, amplified target sequences obtained using the disclosed methods, and compositions can be used in various processes including nucleic acid sequencing and used to detect the presence of genetic variants and/or expression levels of one or more targeted sequences associated with immune response.Type: ApplicationFiled: September 22, 2017Publication date: November 28, 2019Inventors: Ann MONGAN, Alex ATKINS
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Publication number: 20190360054Abstract: The present disclosure relates to a method of diagnosing metastatic cancer or taxane-based drug-resistant cancer, including: measuring an LMCD1 expression level from a complex formed by bringing, into contact with a sample isolated from a subject, an antibody, peptide, protein, or combination thereof that specifically binds to an LMCD1 protein or a fragment thereof; or a probe, primer, nucleotide, or combination thereof that specifically binds to a nucleotide sequence encoding the LMCD1 protein; and comparing the measured LMCD1 expression level of the sample with a measured LMCD1 expression level of a control, and a kit for diagnosing metastatic cancer or taxane-based drug-resistant cancer, including a composition for diagnosing metastatic cancer or taxane-based drug-resistant cancer.Type: ApplicationFiled: May 15, 2019Publication date: November 28, 2019Applicants: AICT (ADVANCED INSTITUTES OF CONVERGENCE TECHNOLOGY), THERAGEN ETEX CO., LTDInventors: Seong Jin KIM, Jin Ah PARK, Eun Jin BAE
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Publication number: 20190360055Abstract: The invention relates to cell survival assays. Specifically, the present invention relates to identification and quantification of cell-free DNA (cfDNA) for quantification of cell survival in a sample.Type: ApplicationFiled: May 23, 2019Publication date: November 28, 2019Inventor: Tim Lautenschlaeger
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Publication number: 20190360056Abstract: The present invention relates to methods and compositions for determining whether a subject having prostate cancer is at greater risk of developing progressive disease, and methods of treating the subjects. It is based, at least in part, on the discovery that approximately 90% of men carrying at least one of the following fusion genes: TRMT11-GRIK2, SLC45A2-AMACR, MTOR-TP53BP1, LRRC59-FLJ60017, TMEM135-CCDC67 and CCNH-C5orf30 experienced prostate cancer recurrence, metastases and/or prostate cancer-specific death after radical prostatectomy (each examples of “progressive prostate cancer”), while these outcomes occurred in only 36% of men not carrying any of these fusion genes. It is also based, at least in part, on the discovery that no patient studied survived five years without recurrence if their primary prostate cancer contained a TRMT11-GRIK2 or MTOR-TP53BP1 fusion gene. It is also based, at least in part, on the discovery that the protein encoded by the MAN2A1-FER fusion gene exhibits kinase activity.Type: ApplicationFiled: May 29, 2019Publication date: November 28, 2019Applicant: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATIONInventors: Jianhua Luo, Yangping Yu, Joel B. Nelson, George Konstantine Michalopoulos, Chien-Cheng Tseng, Ying Ding
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Publication number: 20190360057Abstract: Patients with ErbB2-overexpressing cancers can be given an ErbB2 targeting agent as a therapeutic regimen but not all patients are responsive. The present invention concerns the diagnostic, prognostic and therapeutic methods and compositions for evaluating potential efficacy of an ErbB2 targeting agent in ErbB2-overexpressing cancers by evaluating PTEN expression, which is predictive of responsiveness or resistance to ErbB2 targeting agents such as trastuzumab. Low PTEN expression is predictive of a patient who will respond poorly to trastuzumab.Type: ApplicationFiled: June 18, 2019Publication date: November 28, 2019Applicant: BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEMInventors: Dihua Yu, Xiaoyan Zhou, Yoichi Nagata, Francisco J. Esteva, Aysegul A. Sahin
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Publication number: 20190360058Abstract: Provided herein is technology for colorectal neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of colorectal neoplasia in 1) individuals at, older or younger than 50 years of age, or 2) individuals having Lynch Syndrome.Type: ApplicationFiled: July 18, 2019Publication date: November 28, 2019Inventors: David A. Ahlquist, William R. Taylor, John B. Kisiel, Tracy C. Yab, Douglas W. Mahoney
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Publication number: 20190360059Abstract: The invention is a method of predicting recurrence of colorectal cancer in a patient following surgery, the method comprising analysis of circulating tumor DNA from a patient's sample.Type: ApplicationFiled: August 7, 2019Publication date: November 28, 2019Inventors: Hans-Peter Adams, Aarthi Balasubramanyam, Bernd Hinzmann, John Lee, John Palma, Andre Rosenthal, Urich-Peter Rohr
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Publication number: 20190360060Abstract: The invention is a method of predicting response to therapy in a colorectal cancer patient, the method comprising analysis of circulating tumor DNA from a patient's sample.Type: ApplicationFiled: August 7, 2019Publication date: November 28, 2019Inventors: John Lee, John Palma, Ulrich-Peter Rohr, Lijing Yao, Stephanie Yaung
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Publication number: 20190360061Abstract: Methods and kits for identifying a subject having pre-cancerous advanced polyps or colorectal cancer based on the expression profile(s) of specific mRNA biomarkers. Methods and kits for diagnosing, preventing, managing therapy, monitoring and identifying predisposition to colorectal cancer.Type: ApplicationFiled: August 13, 2019Publication date: November 28, 2019Inventors: Dana COHEN, Vardit MOSHAYOFF, Ouriel FAKTOR
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Publication number: 20190360062Abstract: The present invention relates to a quenching apparatus capable of quickly reversing a flow of cooling gas flowing in a chamber. The quenching apparatus includes: a chamber provided with a gas inlet and a gas outlet and having an object charged therein; a pair of flow generating means disposed on both sides of the chamber and generating a flow of gas in the chamber; and a flow direction reversing means disposed in the chamber and reversing a flow direction of gas to make the gas flow in any one of a first flow direction in which the gas flows from top to bottom of the object and a second flow direction in which the gas flows from the bottom to the top of the object.Type: ApplicationFiled: February 16, 2017Publication date: November 28, 2019Inventor: Won Ki CHUNG
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Publication number: 20190360063Abstract: Embodiments of the present disclosure are directed to coiled steel tubes and methods of manufacturing coiled steel tubes. In some embodiments, the final microstructures of the coiled steel tubes across all base metal regions, weld joints, and heat affected zones can be homogeneous. Further, the final microstructure of the coiled steel tube can be a mixture of tempered martensite and bainite.Type: ApplicationFiled: August 12, 2019Publication date: November 28, 2019Applicant: TENARIS COILED TUBES, LLCInventors: Martin Valdez, Jorge Mitre, Bruce A. Reichert
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Publication number: 20190360064Abstract: Embodiments of the present disclosure are directed to coiled steel tubes and methods of manufacturing coiled steel tubes. In some embodiments, the final microstructures of the coiled steel tubes across all base metal regions, weld joints, and heat affected zones can be homogeneous. Further, the final microstructure of the coiled steel tube can be a mixture of tempered martensite and bainite.Type: ApplicationFiled: August 12, 2019Publication date: November 28, 2019Applicant: TENARIS COILED TUBES, LLCInventors: Martin Valdez, Jorge Mitre, Bruce A. Reichert