Abstract: Method for studying constituents of individual molecular complexes by labelling the molecules belonging to the same complex with at least one set of molecular constructs, wherein each set member includes a Unique Identifying Sequence (UIS), which is a nucleic acid sequence unique for each set member, and at least one Common Tag Sequence (CTS), which is a nucleic acid sequence common to all set members, by: attaching the molecular construct to the complex by ligating or hybridizing the molecular construct to a nucleic acid molecule of the complex, or ligating or hybridizing the tag to a nucleic acid linked to an affinity binder that binds specifically to a constituent of the complex; labelling the molecules belonging to the same complex using the molecular construct tags as primers or templates in a nucleic acid polymerization reaction; and analyzing the composition of the complex by analyzing combinations of UISs and CTSs.

Abstract: Provided are methods and systems for detecting formation of nucleotide-specific ternary complexes comprising a DNA polymerase, a nucleic acid, and a nucleotide complementary to the templated base of the primed template nucleic acid. The methods and systems facilitate determination of the next correct nucleotide without requiring chemical incorporation of the nucleotide into the primer. These results can even be achieved in procedures employing unlabeled, native nucleotides.

Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

Abstract: Plasmonics-active nanoprobes are provided for detection of target biomolecules including nucleic acids, proteins, and small molecules. The nucleic acids that can be detected include RNA, DNA, mRNA, microRNA, and small nucleotide polymorphisms (SNPs). The nanoproprobes can be used in vito in sensitive detection methods for diagnosis of diseases and disorders including cancer. Multiplexing can be performed using the nanoprobes such that multiple targets can be detected simultaneously in a single sample. The methods of use of the nanoprobes include detection by a visible color change. The nanoprobes can be used in vivo for treatment of undesirable cells in a subject.

Abstract: The present disclosure provides a new protocol for sequencing the 3? end of messenger RNA (mRNA). The present disclosure can be very favorably used in analyzing the repetitive sequences of nucleic acids, which are difficult to analyze by current sequencing methods, especially, homopolymeric sequences (poly[A] sequence) of mRNA. The present disclosure has significantly improved sensitivity to mRNA compared with an existing method, thereby obtaining a lot of genetic information from a small amount of sample. The method of the present disclosure reduces the time and cost for sequencing the 3? end of mRNA and can be applied to various samples, and thus, can be used as a useful tool in the study of RNA synthesis/degradation and protein production in association with all life phenomena, including embryogenesis, cancer, and neurotransmission.

Abstract: The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.

Abstract: Disclosed are a composition for PCR including polyethylene glycol-engrafted nano-sized graphene oxide (PEG-nGO), the composition for PCR being capable of increasing the efficiency and specificity of PCR and shortening PCR time, and a PCR method using the same.

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

Abstract: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3?-position of the deoxyribose.

Abstract: The present invention relates to systems and methods for sequencing nucleic acids, including sequencing nucleic acids in fluidic droplets. In one set of embodiments, the method employs sequencing by hybridization using droplets such as microfluidic droplets. In some embodiments, droplets are formed which include a target nucleic acid, a nucleic acid probe, and at least one identification element, such as a fluorescent particle. The nucleic acid probes that hybridize to the target nucleic acid are determined, in some instances, by determining the at least one identification element. The nucleic acid probes that hybridize to the target nucleic acid may be used to determine the sequence of the target nucleic acid. In certain instances, the microfluidic droplets are provided with reagents that modify the nucleic acid probe. In some cases, a droplet, such as those described above, is deformed such that the components of the droplets individually pass a target area.

Abstract: Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.

Abstract: The present invention is based on the use of the H3K9me2 methylation levels in plant genomes to predict transgene silencing, transgene stability, and/or transgene expression level. Provided are methods and/or systems for generating whole-genome H3K9me2 maps and its use with an assigned threshold value for predicting gene silencing. The methods and/or systems provided herein can be used in high-throughput setting for screening large number of transformed event in a relatively short period of time as compared to existing technologies.

Abstract: A previously unknown T cell receptor (TCR) activation pathway dependent on the aryl hydrocarbon receptor conferring resistance to calcineurin inhibitors and mTOR inhibitors is disclosed, including application of this pathway to the diagnosis and treatment of certain disease states refractory to treatment with calcineurin inhibitors. This alternative TCR activation pathway uniquely exists in a subset of CD8 T cells expanded in the setting of chronic rejection or rheumatoid arthritis. Expansion of this newly discovered calcineurin and mTOR inhibitor resistant CD8 T cell subset in humans can be quantified by measuring levels of certain biomarkers in the circulating CD8 T cell pool, such as Pla2g4a, to diagnose disease states mediated thereby. Additionally, methods for diagnosing ongoing active inflammation mediated by this resistant CD8 T cell subset in either chronic rejection or rheumatoid arthritis are provided, which comprise measuring levels of the biomarker Scin in the circulating CD8 T cell pool.

Abstract: Compositions and methods relating to potentially pathogenic mutations in the nucleotide sequence of a human NAGLU gene. Some NAGLU gene variants have been discovered to be associated with reduced N-acetyl-?-D-glucosaminidase (NAGLU) activity.

Abstract: A method of diagnosing a disease associated with a DNA repeat sequence is disclosed. The method comprises: (a) determining the number of repeats of a DNA sequence in DNA molecules of a sample of the subject; and (b) determining the CpG methylation status of the DNA molecules, wherein the number of repeats of the DNA sequence and the CpG methylation status of the DNA molecules is indicative of the disease.

Abstract: Described are mismatch repair (MMR-)deficient tumors. Markers are presented herein having a high sensitivity to detect whether a tumor is mismatch repair deficient or not. The markers are particularly mutations in microsatellite regions. Accordingly, methods and materials are provided for diagnosing microsatellite instability of a tumor. Such a method comprises determining the presence of these markers. Further, kits are provided to detect the presence of these markers (or subsets thereof) in a sample.

Abstract: The present invention provides a kit or device for the detection of biliary tract cancer, and a method for detecting biliary tract cancer. The present invention relates to a kit or device for the detection of biliary tract cancer, comprising a nucleic acid capable of specifically binding to miRNA in a sample of a subject, and a method for detecting biliary tract cancer, comprising measuring the miRNA in vitro.

Abstract: An apparatus and a method for diagnosing a tumor are provided. A histogram generating section generates a histogram from a result of a measurement of fluorescence intensity of a suspension produced from a target sample. The histogram indicates a relation between the fluorescence intensity and the number of cells. A determination section detects an S phase fluorescence intensity range corresponding to S phase cells based on the fluorescence intensity corresponding to a peak of the number of cells in the histogram, detects an abnormal fluorescence intensity range corresponding to tumor cells, based on a change in the number of cells in the S phase fluorescence intensity range, and performs a diagnosis of a tumor in the target sample based on an index of the number of cells in a range defined by excluding the abnormal fluorescence intensity range from the S phase fluorescence intensity range.

Abstract: A method of identifying gene expression associated with recurrence free survival in a subject with cancer, comprising: a) assaying a sample from a subject diagnosed with cancer for the presence of altered gene expression of one or more genes selected from the group consisting of ADK, AP2B1, AVL9, CANX, DBT, DHRS7, DONSON, FAM190B, FGFR1, FOXN3, FZD5, GGH, GM2A, IGFBP5, ITSN2, LAMC1, LIFR, METTL7A, MT1F, MT1G, MT1P2, MT1X MT2A, NAB1, NCOA1, NCOR1, PAPOLA, PPME1, PPP1R13L, PRKAR2A, RABEP1, RBBP8, SGPL1, SIRT1, SNX2, SREK1, TAF1B, TMED5, and ZMIZ2; and b) identifying an outcome of decreased likelihood of recurrence free survival when altered gene expression relative to the level in a non-cancerous sample is present.

Abstract: Methods that rapidly, sensitively, and specifically detect mutations in IDH1/2 and the TERT promoter employ amplification of particular portions of the genes that experience frequent and exquisitely localized mutations. The ability to distinguish between sequences that differ only by one nucleotide and which may be present in very low ratios is essential for such an assay.

Abstract: The technology described herein relates to assays and methods for the diagnosis, prognosis, and/or treatment of melanoma, e.g. relating to measuring the level of neurophilin-2 (NRP-2) mRNA expressed in melanoma cells. In some embodiments, the level of NRP-2 can be normalized to the level of Melan-A (MART) mRNA.

Abstract: Various embodiments are directed to applications (e.g., classification of biological samples) of the analysis of the count, the fragmentation patterns, and size of cell-free nucleic acids, e.g., plasma DNA and serum DNA, including nucleic acids from pathogens, such as viruses. Embodiments of one application can determine if a subject has a particular condition. For example, a method of present disclosure can determine if a subject has cancer or a tumor, or other pathology. Embodiments of another application can be used to assess the stage of a condition, or the progression of a condition over time. For example, a method of the present disclosure may be used to determine a stage of cancer in a subject, or the progression of cancer in a subject over time (e.g., using samples obtained from a subject at different times).

Abstract: The present disclosure provides methods for profiling a microbiome and therapeutic compositions for treatment. Additionally, the methods, systems, compositions and kits provided herein are directed to assessing or predicting health status in a subject. Some of the embodiments include generating a report.

Abstract: Nucleic acid sequences for predicting and controlling shell phenotype in palm.

Abstract: A blast furnace control system may include a hardware processor that generates a deep learning based predictive model for forecasting hot metal temperature, where the actual measured HMT data is only available sparsely, and for example, measured at irregular interval of time. HMT data points may be imputed by interpolating the HMT measurement data. HMT gradients are computed and a model is generated to learn a relationship between state variables and the HTM gradients. HMT may be forecasted for a time point, in which no measured HMT data is available. The forecasted HMT may be transmitted to a controller coupled to a blast furnace, to trigger a control action to control a manufacturing process occurring in the blast furnace.

Abstract: A low thermal expansion superalloy is composed of, in mass %, 0.1% or less of C, 0.1-1.0% of Si, 1.0% or less of Mn, 25-32% of Ni, more than 18% but less than 24% of Co, more than 0.25% but 1.0% or less of Al, 0.5-1.5% of Ti, more than 2.1% but less than 3.0% of Nb, 0.001-0.01% of B and 0.0005-0.01% of Mg, with the balance of Fe and unavoidable impurities, while satisfying Mg/S?1, 52.9?1.235Ni+Co<55.8%, (Al+Ti+Nb) is 3.5-5.5%, and the F value is 8% or less. In the superalloy, a granular intermetallic compound containing Si, Nb, and Ni alone or in a total amount of 36 mass % or more is precipitated at a grain boundary of an austenite matrix, and an intermetallic compound including a larger concentration of Ni, Al, Ti, and Nb and having 50 nm or smaller of an average diameter is precipitated in the austenite matrix.

Abstract: An apparatus and method for hardening and compacting an inner face of a cylindrical opening of a structure. The apparatus and method is directed towards the hardening and compaction of an elongated cylindrical opening by surrounding the inner face of the cylindrical opening with a glass tube, and initiating an explosive volume expansion of the glass. This in turn, creates a compressive force that acts on the inner face to compact and harden the surface.

Abstract: A method of forming a gun barrel is disclosed that includes cold gas-dynamic spraying one or more coatings onto a mandrel. The method may also include heat treating the coating layer(s), contouring the outer coating layer, applying a ceramic top coating to the contoured outer coating layer of the gun barrel, and/or sealing the gun barrel with a liquid metal sealer. The method may also include removing the mandrel.

Abstract: A high-strength galvanized steel sheet having a chemical composition containing, by mass %, C: 0.07% to 0.25%, Si: 0.01% to 3.00%, Mn: 1.5% to 4.0%, P: 0.100% or less, S: 0.02% or less, Al: 0.01% to 1.50%, N: 0.001% to 0.008%, Ti: 0.003% to 0.200%, B: 0.0003% to 0.

Abstract: A hydrometallurgical process for the treatment of polymetallic ores and sulphide concentrates of copper and zinc, and by-products of lead and zinc from smelting plants, treated independently and/or as mixtures thereof, which contain relevant amounts of lead, copper, zinc, iron, gold and silver, such as the matte-speiss mixture of lead foundries, and copper cements from the purification processes of electrolytic zinc plants. The process allows the recovery of metallic copper, zinc, copper as copper and zinc basic salts, which may be hydroxides, carbonates, hidroxysulphates or mixtures thereof; the production of stable arsenic residues; and the effective and efficient recovery of Pb, Au and Ag as a concentrate of lead sulphide and/or lead, Au, and Ag sulphate.

Abstract: There are provided methods and systems for creating multi-phase covetics. For example, there is provided a process for making a composite material. The process includes forming a multi-phase covetic. The forming includes heating a melt including a metal in a molten state and a carbon source to a first temperature threshold to form metal-carbon bonds. The forming further includes subsequently heating the melt to a second temperature threshold, the second temperature threshold being greater than the first temperature threshold. The second temperature threshold is a temperature at or above which ordered multi-phase covetics form in the melt.

Abstract: There are provided methods and systems for creating multi-phase covetics. For example, there is provided a process for making a composite material. The process includes forming a multi-phase covetic. The forming includes heating a melt including a metal in a molten state and a carbon source to a first temperature threshold to form metal-carbon bonds. The forming further includes subsequently heating the melt to a second temperature threshold, the second temperature threshold being greater than the first temperature threshold. The second temperature threshold is a temperature at or above which ordered multi-phase covetics form in the melt.

Abstract: An aluminum alloy product and method for producing the aluminum alloy product that, in some embodiments, includes an aluminum alloy strip having at least 0.8 wt. % manganese, at least 0.6 wt % iron, or at least 0.8 wt. % manganese and at least 0.6 wt % iron. A near surface of the aluminum alloy strip, in some embodiments, is substantially free of large particles having an equivalent diameter of at least 50 micrometers and includes small particles. Each small particle, in some embodiments, has a particular equivalent diameter that is less than 3 micrometers, and a quantity per unit area of the small particles having the particular equivalent diameter is at least 0.01 particles per square micrometer at the near surface of the aluminum alloy strip.

Abstract: Aluminum-Iron-Zirconium alloys that exhibit improved electrical and mechanical properties.

Abstract: A low alloy high nitrogen steel includes iron and, by weight 0.14-0.60% nitrogen (N); 0.08-0.28% carbon (C); 0.10-2.20% nickel (Ni); 0.25-2.00% manganese (Mn); 1.20-2.70% chromium (Cr); 0.45-1.50% tungsten (W); not more than 0.05% molybdenum (Mo); not more than 0.02% vanadium (V); not more than 0.60% silicon (Si); not more than 0.10% copper (Cu); not more than 0.02% titanium (Ti); not more than 0.02% niobium (Nb); not more than 0.008% aluminum (Al); and not more than 0.02% of any other element with not more than 0.10% total other elements, wherein cobalt (Co) is substitutable for any part of the nickel.

Abstract: A high strength steel comprises, during intercritical annealing, about 20-80% volume ferrite and 20-80% austenite, and wherein the Ms temperature calculated for the austenite phase during intercritical anneal ?100° C. The high strength steel exhibits a tensile elongation of at least 20% and an ultimate tensile strength of at least 880 MPa. The high strength steel may comprise 0.20-0.30 wt % C, 3.0-5.0 wt % Mn, with Al and Si additions such that the optimum intercritical temperature is above 700° C.

Abstract: The invention relates to an alloy based on iron comprising, by weight: 35%?Ni?37% trace amounts?Mn?0.6% trace amounts?C?0.07% trace amounts?Si?0.35% trace amounts?Cr?0.5% trace amounts?Co?0.5% trace amounts?Mo<0.5% trace amounts?S?0.0035% trace amounts?O?0.0025% 0.011%?[(3.138×Al+6×Mg+13.418×Ca)?(3.509×O+1.770×S)]?0.038% 0.0003%<Ca?0.0015% 0.0005%<Mg?0.0035% 0.0020%<Al?0.0085% the remainder being iron and residual elements resulting from the elaboration.

Abstract: Provided is austenitic heat-resisting cast steel that is excellent in both of the heat resistance and the machinability. Austenitic heat-resisting cast steel, includes: C: 0.1 to 0.4 mass %; Si: 0.8 to 2.5 mass %; Mn: 0.8 to 2.0 mass %; S: 0.05 to 0.30 mass %; Ni: 5 to 20 mass %; N: 0.3 mass % or less; Zr: 0.01 to 0.20 mass %; Ce: 0.01 to 0.10 mass %; one type or more of the elements selected from the following groups of (i) to (iii), at least including (i), (i) Cr: 14 to 24 mass %, (ii) Nb: 1.5 mass % or less, and (iii) Mo: 3.0 mass % or less; and Fe and inevitable impurity as a remainder.

Abstract: Provided is a material for a cold-rolled stainless steel sheet having a chemical composition containing, by mass %, C: 0.01% to 0.05%, Si: 0.02% to 0.75%, Mn: 0.1% to 1.0%, P: 0.04% or less, S: 0.01% or less, Cr: 16.0% to 18.0%, Al: 0.001% to 0.10%, N: 0.01% to 0.06% and the balance being Fe and inevitable impurities. The material has a metallographic structure including a martensite phase having an area ratio of 5% to 50% and the balance being a ferrite phase. A ferrite phase in portions extending from surface layers of front and back surfaces of a steel sheet has an average grain diameter of 20 ?m or more and 50 ?m or less, and a ferrite phase in a central portion of the sheet includes an unrecrystallized ferrite phase.

Abstract: A method for producing an enhanced property integrally bladed rotor includes solution heat treating a stub-containing rotor hub forging; water quenching the solution heat treated stub-containing rotor hub; aging the water quenched stub-containing rotor hub forging; linear friction welding airfoils onto each of a multiple of stubs of the stub-containing rotor hub forging; and concurrently stress relieving the linear friction welds of each of the multiple of stubs within a predefined area while ensuring that a hub inner diameter does not exceed a predetermined temperature.

Abstract: Titanium alloys formed into a part or component used in applications where a key design criterion is the energy absorbed during deformation of the part when exposed to impact, explosive blast, and/or other forms of shock loading is described. The titanium alloys generally comprise a titanium base with added amounts of aluminum, an isomorphous beta stabilizing element such as vanadium, a eutectoid beta stabilizing element such as silicon and iron, and incidental impurities. The titanium alloys exhibit up to 70% or more improvement in ductility and up to a 16% improvement in ballistic impact resistance over a Ti-6Al-4V alloy, as well as absorbing up to 50% more energy than the Ti-6Al-4V alloy in Charpy impact tests. A method of forming a part that incorporates the titanium alloys and uses a combination of recycled materials and new materials is also described.

Abstract: Nitrogen (N) absorption and diffusion treatments are performed for the inner and/or outer surfaces of austenite stainless steel pipe materials in N gas atmosphere at temperatures near 1,100° C. to obtain nitrided stainless steel pipe materials having 0.25˜1.7% (mass) of solid solution nitrogen (N) including a gradient structure formed within the pipe wall in which the concentration of solid solution N continuously decreases gradually from the surface. The solid solution N present in the gradient structure promotes short range ordering (SRO) of substitutional alloying elements leading to homogenization of distribution of alloying elements in the austenite phase, generating an extremely high proof strength (yield strength) about 3 times as high as that of conventional austenite stainless steel pipe materials and enhancing characteristic of anti-hydrogen gas embrittlement (anti-HGE) so as to be suitable for use in a high pressure hydrogen tank utilized in hydrogen cell vehicle (FCV) and a liquid hydrogen tank.

Abstract: Devices, systems, and methods for detection of an analyte in a sample are disclosed. In some embodiments, an optical sensor can include a metallic layer and a plurality of dielectric pillars extending through the metallic layer. A plurality of regions of concentrated light can be supported in proximity to the ends of the plurality of dielectric pillars when a surface of the metallic layer is illuminated. Concentrated light within one or more of these regions can interact with an analyte molecule, allowing for detection of the analyte.

Abstract: A method for a defined surface treatment of a first carbon coating applied to a surface of a component The first carbon coating is brought into touching contact with at least one second carbon coating that is formed on a surface of a tool or second component and the two carbon coatings are moved relatively with one another so that the first carbon coating is smoothed by the second carbon coating. The first carbon coating and/or the second carbon coating are formed from a-C (amorphous carbon) or ta-C (tetrahedrally bonded amorphous carbon).

Abstract: A film formation apparatus includes a carrying unit circulating and carrying an electronic component in a sputtering chamber, a film formation processing unit including a mount surface and a mount member which is mounted on the mount surface, and on which the electronic component is placed. The mount member includes a holding sheet having an adhesive surface, and a non-adhesive surface, and a sticking sheet having a first sticking surface with adhesiveness sticking to the non-adhesive surface, and a second sticking surface with adhesiveness sticking to the mount surface of the tray. The adhesive surface includes a pasting region for pasting the electronic components. The first sticking surface sticks across an entire region of the non-adhesive surface corresponding to at least the pasting region to provide a file formation apparatus which employs a simple structure that is capable of suppressing heating of electronic components.

Abstract: A device for atomic layer deposition includes: a film deposition chamber; a stage installed inside the film deposition chamber; a susceptor that holds, on the stage, a substrate; a mask disposed on the substrate, the mask being sized to encompass the substrate; a mask pin that supports the mask; and a mask pin hole bored through the stage and the susceptor vertically, and allows the mask pin to be inserted in a vertically movable manner, wherein the susceptor has a susceptor body having a holding surface of the substrate, and a susceptor peripheral edge located around the susceptor body and having a height lower than the holding surface, the mask pin hole is opened in the susceptor peripheral edge, and in the susceptor peripheral edge, an inert gas supply port that releases gas upward is provided around the holding surface in a surrounding area of the mask.

Abstract: Embodiments described herein relate to a protective coating that protects an underlying chamber component (i.e. the object upon which the coating is being deposited) from corrosion or deterioration within a corrosive environment.

Abstract: A substrate processing apparatus includes: a process chamber in which a substrate is processed; a substrate support configured to support the substrate in the process chamber; a plurality of reaction gas supply holes formed in a wall of the process chamber opposite to a substrate mounting surface of the substrate support; a reaction gas supply pipe that is fixed to the process chamber and communicates to each of the reaction gas supply holes; a plurality of reaction gas suppliers, each including a plasma generator installed on an upstream side of the reaction gas supply pipe; a plasma controller that is connected to the plasma generator and is configured to individually control a plurality of plasma generators; and a controller configured to control the substrate support, the plurality of reaction gas suppliers, and the plasma controller.

Abstract: Protective coatings on an aerospace component and methods for depositing the protective coatings are provided. A method for depositing a coating on an aerospace component includes exposing an aerospace component to a first precursor and a first reactant to form a first deposited layer on a surface of the aerospace component by a chemical vapor deposition (CVD) process or a first atomic layer deposition (ALD) process and exposing the aerospace component to a second precursor and a second reactant to form a second deposited layer on the first deposited layer by a second ALD process, where the first deposited layer and the second deposited layer have different compositions from each other.