Abstract: Methods and formulations for preparing low non-specific binding surfaces are described, and the prepared surface can provide improved performance for nucleic acid detection and base calling applications. The surface provides more accurate nucleic acid detection, enhanced contrast to noise ratio, and better data collection.

Abstract: The present disclosure is directed to unsymmetrical cyanine dyes comprising a substituted benzazolium ring system linked by a methine bridge to a quinolinium ring that contains a heteroatom. Compounds of formula (I) are provided herein. The compounds can be useful for fluorescent detection or quantification of nucleic acids. Related methods, uses, and kits are disclosed.

Abstract: Methods and compositions to minimize barcode exchange during the preparation of barcoded next-generation sequencing libraries prepared from a single cell. The methods utilize oligonucleotides containing a 3?-terminated blocking group or sequences that prevent amplification or extension.

Abstract: [Problem] An objective of the present invention is to develop a new type of method for nucleic acid detection that can be suitably used for detection of short nucleic acids. [Solution] The present invention provides a method for nucleic acid detection including i) hybridizing a detection target nucleic acid 1 and a primer for nucleic acid detection 3 to form a DNA-RNA complex, ii) removing a first polynucleotide segment 301 from the primer for nucleic acid detection 3 by degrading at least a portion of the DNA-RNA complex with a nuclease 4, and iii) hybridizing the primer for nucleic acid detection 3 to a template nucleic acid 2 to perform a reaction for amplifying the template nucleic acid 2 by a polymerase 5.

Abstract: Methods of uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs and/or cDNAs.

Abstract: The present invention relates to a method for enrichment of target DNA with high GC content based on target sequence capture and multiple displacement amplification, as well as a kit suitable for this method. The present invention also relates to a method for constructing a sequencing library of target DNA with high GC content based on the enrichment method of the present invention.

Abstract: The invention relates to an active substance-coated rod-shaped sample transfer tool, the preparation thereof, and the use thereof for transferring nucleic acid-containing sample material into reaction mixtures of enzymatic detection reactions.

Abstract: Methods and devices for forming droplets are provided. In certain embodiments, the methods and devices form droplets having different diameters.

Abstract: Sequences having specificity for Mycoplasma and related Mollicutes genus strains and uses thereof. Methods of use include detection of samples contaminated with Mycoplasma. Kits are provided and comprise one or more oligonucleotides for the detection of Mycoplasma and related Mollicutes genus strains.

Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.

Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5? of the target-specific primer region; and a second stem forming region 3? of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

Abstract: The invention relates to a process for pre-concentration and detection of at least one single-stranded nucleic acid target molecule, the process comprising steps of generating a flow of liquid comprising at least one magnetic nanoparticle in a micro-channel and a plurality of single-stranded nucleic acid probe molecules attached to the nanoparticle, of generating an alternating magnetic field in the part of the micro-channel by means of the electromagnet, the magnetic field having an intensity and frequency that are suitable for causing magnetic hyperthermia of the nanoparticles so as to cause denaturing of the duplex formed by the single-stranded nucleic acid target molecule and the single-stranded nucleic acid probe molecule, and detection of the single-stranded target molecule dispersed in the liquid.

Abstract: The disclosed disclosure is related to methods, compositions, and kits for targeting Adenovirus, Metapneumovirus, and/or Rhinovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers. Methods include uniplex and multiplex amplification and detection reactions.

Abstract: The invention relates to a method for the duplication of nucleic acids by means of a polymerase chain reaction, in the case of which a cycle consisting of the steps of denaturing, annealing and elongation is repeatedly performed. In one embodiment, in at least one passage of the cycle, the quotient of the duration of effect tA and the reaction volume Vr irradiated by the energy source is less than 1 seconds per microliter. In another embodiment, in at least one passage of the cycle, the ratio of the duration of effect (tA) and the duration of the PCR cycle (tc) is less than 20%. In certain embodiments, the yield (g) of nucleic acids at the end of at least one of the passages of the cycle is less than 80% of the nucleic acids present at the start of the passage.

Abstract: Described herein are systems and methods which utilize an array of wells to isolate pathogens and nucleic acid detection techniques to accurately and rapidly detect pathogens in fluid samples, even in very low concentrations, including from solid or semi-solid samples that have been fluidized. The provided systems and methods dry the fluid sample to deposit a fraction of the total volume in a number of wells and perform nucleic acid detection on individual wells to detect even individual pathogens and provide a quantitative analysis of the amount of pathogen within the sample. Also provided are methods and systems for precise delivery of dried materials, including biomolecules that are enzymes of use in the process, to microwells.

Abstract: The present invention provides methods for increasing polymerase processivity. In some aspects the invention includes providing a polymerase-nucleic acid complex having a nucleic acid comprising a template strand, and the template strand is entrapped in the polymerase through anchors that are covalently connected to each other across the nucleic acid binding cleft of the polymerase. In some cases the anchors comprise cysteines.

Abstract: A method for sequencing a population of nucleic acids, which includes (a) binding the population of nucleic acids with a fractionally labeled mixture of nucleotides, thereby forming a fractionally labeled population of nucleic acids, wherein the mixture includes nucleotide cognates for a common base type in the templates, and wherein a fraction of the nucleotide cognates for the common base type in the mixture are exogenously labeled nucleotides that produce a signal that is not produced by other nucleotide cognates for the common base type in the mixture; (b) detecting the signal from the fractionally labeled population of nucleic acids; and (c) repeating (a) and (b) using a second mixture of the fractionally labeled nucleotides, wherein the fraction of the exogenously labeled nucleotides is higher in the second mixture.

Abstract: A method for detecting the presence or absence of a target polynucleotide in a sample is described.

Abstract: The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5?-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.

Abstract: The present invention relates to a kit for determining susceptibility to an allergic reaction for a subject. The kit includes a probe for identifying a target biomarker, and an instruction that indicates the subject's susceptibility to the allergic reaction. The present invention also relates to a method of predicting susceptibility to an allergic reaction for a subject and treating and/or preventing the allergic reaction.

Abstract: Activity-dependent gene pairs as therapeutic targets and methods and devices to identify the same are provided. The methods and devices allow transcriptome-wide analysis of regulatory long non-coding RNAs (lncRNAs), matched with differentially expressed protein-coding genes (mRNAs) and/or with other lncRNAs. The described methods and devices allow analysis of these activity-dependent gene pairs as therapeutic targets in a number of clinical conditions associated with altered electrical brain activity, including epilepsy.

Abstract: Methods of treating patients having decreased bone mineral density and/or osteoporosis, methods of identifying subjects having an increased risk of developing decreased bone mineral density and/or osteoporosis, and methods of diagnosing decreased bone mineral density and/or osteoporosis in a human subject, comprising detecting the presence of Matrix Extracellular Phosphoglycoprotein (MEPE) predicted loss-of-function variant nucleic acid molecules and polypeptides in a biological sample from the patient or subject, are provided herein.

Abstract: Methods of treating patients having varicose veins, methods of identifying subjects having an increased risk of developing varicose veins, and methods of diagnosing varicose veins in a human subject, comprising detecting the presence of Piezo Type Mechanosensitive Ion Channel Component 1 (PIEZO1) predicted loss-of-function variant nucleic acid molecules and polypeptides in a biological sample from the patient or subject, are provided herein.

Abstract: The invention relates to improved methods, devices, and kits for identifying and implementing an appropriate treatment regimen for subjects suffering from hypertension.

Abstract: A method is useful for predicting a response of a malignant disease to an immunotherapy which inhibits the PD-1 immune checkpoint signaling pathway. The method is characterized in that at least a part of an immunoregulatory gene selected from CTLA4, CD86, CD28, CD80 and/or ICOS of cells of the malignant disease and/or of immune cells interacting with cells of the malignant disease is subjected to a DNA methylation analysis, and the result is used to predict the response of the malignant disease to the immunotherapy.

Abstract: The present invention relates to the classification of cancers, in particular prostate cancers, using samples from patients. In particular, the invention provides methods for identifying potentially aggressive prostate cancers to determine which cancers are or will become aggressive (and hence require treatment) and which will remain indolent (and will therefore not require treatment). The present invention is therefore useful to identify patients with a poor prognosis. The specific population of cancer identified by the present invention is referred to herein as DESNT cancer. The invention also provides biomarker panels useful in the diagnosis and prognosis of cancer.

Abstract: Disclosed herein, in certain embodiments, are methods and kits for diagnosing a subject as having hepatocellular carcinoma (HCC) or lung cancer. In some instances, also described herein are methods of determining the prognosis of the subject having HCC or lung cancer. In additional instances, described herein are methods of determining the specific staging of HCC or lung cancer in a subject.

Abstract: The present invention relates to biomarkers associated with breast cancer prognosis. These biomarkers include coding transcripts and their expression products, as well as non-coding transcripts, and are useful for predicting the likelihood of breast cancer recurrence in a breast cancer patient. The present invention also relates to a novel method of identifying intergenic sequences that correlate with a clinical outcome.

Abstract: This document relates to methods and materials for assessing and/or treating mammals (e.g., humans) having one or more polyps (e.g., one or more colon polyps). For example, this document provides methods and materials for determining if a polyp (e.g., a polyp within a mammal having one or more polyps) is likely to recur and/or likely to progress to a cancer. This document also provides methods and materials for treating a mammal having one or more polyps.

Abstract: Provided herein are a kit and method for detecting mutations in CTNNB1, and their use in detection and management of hepatocellular carcinoma (HCC). The kit comprises a first pair of primers, configured to specifically bind sequences flanking the genomic region for amplifying the genomic region in a first PCR reaction; and at least one clamp, each configured to bind to one first allele but not any second allele at an annealing temperature in the first PCR reaction to thereby selectively suppress amplification of the one first allele but still allow amplification of other second allele(s). A method for detecting or monitoring recurrence of HCC is further disclosed, which comprises determining levels of five DNA markers, including CTNNB1 mutations, hTERT mutations, TP53 mutations, RASSF1A methylation, and GSTP1 methylation.

Abstract: Values for tumor mutation burden from different samples can be made more comparable to each other or control standards by a normalization regime that takes into account the minor allele fraction of highly rated mutations in a sample. Such analysis can provide an indication where the tumor mutation burden of a test sample lies on a distribution of tumor mutation burdens in a control population, and thus, whether the individual providing the test sample is likely to be amenable to immunotherapy to treat cancer.

Abstract: The present invention provides diagnostic methods, therapeutic methods, and compositions for the treatment of cancer (e.g., a bladder cancer (e.g., UC, e.g., mUC), a kidney cancer, a lung cancer, a liver cancer, an ovarian cancer, a pancreatic cancer, a colorectal cancer, or a breast cancer). The invention is based, at least in part, on the discovery that expression levels of one or more biomarkers described herein in a sample from an individual having cancer can be used in methods of identifying an individual having a cancer who may benefit with an anti-cancer therapy that includes an immunotherapy (e.g., a PD-L1 axis binding antagonist such as an anti-PD-L1 antibody (e.g., atezolizumab)) and a suppressive stromal antagonist (e.g.

Abstract: The present invention relates to methods and compositions for identifying, selecting and/or producing a plant or germplasm having root increased drought tolerance and/or increased yield under non-drought conditions as compared to a control plant. A maize plant, part thereof and/or germplasm, including any progeny and/or seeds derived from a maize plant or germplasm identified, selected and/or produced by any of the methods of the present invention is also provided.

Abstract: The present disclosure provides a composition comprising a panel of probes for detecting one or more viruses in a sample. The panel of probes may be used to detect viruses in a biological sample obtained from a subject.

Abstract: Embodiments disclosed herein provide a pan-tissue cell atlas of healthy and diseased subjects obtained by single cell sequencing. The present invention discloses novel markers for cell types. Moreover, genes associated with disease, including HIV infection and tuberculosis are identified. The invention provides for diagnostic assays based on gene markers and cell composition, as well as therapeutic targets for controlling immune regulations and cell-cell communication of the cell types disclosed herein. In addition, novel cell types and methods of quantitating, detecting and isolating the cell types are disclosed.

Abstract: The present invention discloses a method of preparing oligosaccharide using biomass sugar as raw material. The method places a biomass sugar in a quartz tubular reactor, places the quartz tubular reactor at a temperature-controlled zone of a tubular thermal conversion reactor under the protection of nitrogen gas, controls a thermal conversion time, rapidly quenches a material to obtain an oligosaccharide precursor after thermal conversion ends, then dissolves by adding pure water and purifies through an inorganic ultrafiltration membrane, and finally obtain an oligosaccharide after drying.

Abstract: The present invention relates to a method for manufacturing a tubular product, characterized in that the tubular product is manufactured from steel comprising chromium in the range of 2.5 to 9.5 wt. % and silicon in an amount of more than 1.0 wt. %, and the method comprises the steps of austenitizing, quenching and tempering at a tempering temperature in the range of 300° C. to 550° C. Furthermore, the invention concerns a tubular product produced by this method.

Abstract: The disclosure provides Fe—Cr—Ni—Mo—P—C—B metallic glass-forming alloys and metallic glasses that have a high glass forming ability along with a high thermal stability of the supercooled liquid against crystallization.

Abstract: The present invention relates to a high manganese steel for low temperature applications and a method for manufacturing the same. The high manganese steel contains 0.3 wt % to 0.8 wt % of C, 18 wt % to 26 wt % of Mn, 0.01 wt % to 1 wt % of Si, 0.01 wt % to 0.5 wt % of Al, 0.1 wt % or less of Ti (excluding 0%), 1 wt % to 4.5 wt % of Cr, 0.1 wt % to 0.9 wt % of Cu, 0.03 wt % or less of S (excluding 0%), 0.3 wt % or less of P (excluding 0%), 0.001 wt % to 0.03 wt % of N, 0.004 wt % or less of B (excluding 0%), and a remainder of Fe and other inevitable impurities, wherein a microstructure comprises an austenite single phase structure, and an average grain size of the austenite is 50 ?m or less.

Abstract: A working system includes two or more container which are manufactured separately and previously and then moved to the factories or working plants, and then assembled into the continuous working system in the factories or working plants, for allowing the working system to be easily manufactured and assembled and moved or transported to the required places or positions. The containers each include one or more working zones and an aisle for entering into the working zones selectively. Two of the containers are arranged opposite to each other and parallel to each other, and the other containers are arranged opposite to and parallel to each other and disposed between the other two containers.

Abstract: Provided is an ultra high strength hot-rolled steel sheet including, in percentage by weight: 0.15-0.25% of C, 0.6-2.0% of Si, 1.5-3.0% of Mn, 0.01-0.1% of Al, 0.01-0.5% of Cr, 0.005-0.2% of Mo, 0.001-0.05% of P, 0.001-0.05% of S, 0.001-0.01% of N, 0.003-0.1% of Nb, 0.003-0.1% of Ti, 0.003-0.1% of V, and the remainder being Fe and other unavoidable impurities, and satisfying following relational expressions (1) and (2). [Relational Expression 1] 4.5?[Mn]+12[sol.C]+2.5[Mo]+2[Cr]+300[B]+[V]?5.3. [Relational Expression 2] [sol.C]=[C]?(0.25[Ti]+0.13[Nb]+0.125[Mo]), 0.17?[sol.C]?0.22. (In relational expressions 1 and 2, each element symbol represents the content of each element in wt %.

Abstract: A kind of steel is able to achieve a high elongation with the steel used for hot stamping by means of simple hot stamping process. The formed component has excellent yield strength, tensile strength and elongation. The steel used for hot stamping comprises by weight percent 0.1-0.19% of C, 5.09-9.5% of Mn, 0.11-0.4% of V, and 0-2% Si+Al, wherein the combination of C and V meets one of the following two requirements: 1) 0.1-0.17% of C and 0.11-0.4% of V; and 2) 0.171-0.19% of C and 0.209-0.4% of V.

Abstract: A fabrication method for a press hardened part is provided. A sheet or a steel substrate blank for heat treatment is provided. A pre-coating is applied. The pre-coating has at least one layer of aluminum or aluminum alloy in contact with the steel substrate on at least one of the principal faces of the sheet or blank. Then a polymerized layer is deposited on the pre-coating. The polymerized layer has a thickness between 2 and 30 ?m. The polymerized layer does not contain silicon, has a nitrogen content of less than 1% by weight and carbon pigments in a quantity between 3 and 30% by weight. The blank or the sheet is heated to obtain an interdiffusion between the steel substrate and the pre-coating and to give the steel a partly or totally austenitic structure. Then the blank or the sheet is hot stamped to obtain a part. The part is cooled by holding the part in a stamping tool so that the microstructure of the steel substrate includes, at least in a portion of the part, martensite or bainite.

Abstract: The present disclosure discloses a method and device for removing iron in an iron-containing solution in hydrometallurgy. This method comprises the steps of: adding an iron-containing solution in hydrometallurgy into a reactor through a first homogenizing distributor, controlling concentration of the ferric iron in the reactor below 1 g/L, controlling pH of the solution in the reactor to be 2.5˜4, the temperature to be 65˜100° C., and the reaction duration to be 1˜3 hours, performing solid-liquid separation for the solution after reaction, and removing the iron in the iron-containing solution in hydrometallurgy in the form of goethite.

Abstract: One object of the present disclosure is to provide a production method of magnesium hydride that is free of carbon dioxide and has high production efficiency, a power generation system that does not emit carbon dioxide or radiation using magnesium hydride, and an apparatus for producing magnesium hydride; therefore, the method for producing magnesium hydride of the present disclosure comprises a procedure for irradiating a magnesium compound different from magnesium hydride with hydrogen plasma, and a procedure for depositing a magnesium product containing magnesium hydride on a depositor for depositing magnesium hydride disposed within the range in which hydrogen plasma is present, wherein the surface temperature of the depositor is kept no more than a predetermined temperature at which magnesium hydride precipitates.

Abstract: The invention relates to a method for recovering precious metals from electronic waste utilising biometallurgical techniques. In one aspect, a method of recovering one or more target metals from electronic waste, includes (a) removing at least a portion of non-target material from the electronic waste or grinding to a preselected size particle to give pre-processed electronic waste; (b) contacting the pre-processed electronic waste with a lixiviant such that at least a portion of the target metal(s) dissolve into the lixiviant to produce a pregnant solution; (c) contacting a microorganism with the pregnant solution such that at least a portion of the target metal(s) ions biosorb to the microorganism wherein the microorganism becomes metal laden and the pregnant solution becomes barren; (d) substantially separating the metal laden microorganism from the barren solution; and (e) recovery of the target metal(s) from the metal laden microorganism.

Abstract: A method for treating a lithium ion battery waste according to the present invention is a method for treating a lithium ion battery waste using a converter furnace in a copper smelting process, wherein, prior to a treatment for charging a copper mat produced in a flash smelter in a copper smelting process into a converter furnace and blowing oxygen into the converter furnace to produce crude copper, the lithium ion battery waste is introduced into the converter furnace or a ladle that is used for the charging of the copper mat into the converter furnace and then the lithium ion battery waste is burned with residual heat in the converter furnace or the ladle.

Abstract: A process for recovering valuable products from ore containing boron and lithium, such as jadarite ore, includes an acid digestion step and downstream steps that recover valuable boron-containing and lithium-containing products.

Abstract: Embodiments of the present disclosure include compositions that include magnesium and gadolinium or magnesium and one or more metals.

Abstract: The present invention relates to a steel material for pressure vessels, offshore structures and the like and, more specifically, to a high-strength steel material having excellent low-temperature strain aging impact properties and a method for manufacturing same.