Abstract: Marine gas oil compositions corresponding to fuels and/or fuel blending components are provided that can provide improved friction properties within an engine. Addition of lubricant base stock to a marine gas oil composition can reduce frictional losses within an engine during operation. The benefits in reduction of frictional losses can be observed based on the difference between the indicated mean effective pressure and the actual work delivered by an engine, where the difference corresponds to the frictional mean effective pressure.

Abstract: The invention relates to aqueous polymer dispersions comprising at least one polymer obtainable by the reaction of at least one monomer M1 of the general formula (I): H2C?CH—C(O)OR, wherein R is an unbranched alkyl chain comprising from 18 to 22 carbon atoms, and optionally at least one monomer M2. The invention relates moreover to a method for the preparing of such aqueous polymer dispersion and the use thereof as pour point depressant for crude oil, petroleum, and petroleum products.

Abstract: A regeneration method for a liquefied gas thiol-removing alkaline solution comprising the following steps: performing an oxygenation reaction with respect to a liquefied gas thiol-removing alkaline solution and, at the same time, utilizing a high air-liquid condition to extract a disulfide and a polysulfide into a gas phase, thus completing the separation of the disulfide and the polysulfide from the alkaline solution, and implementing the regeneration of the liquefied gas thiol-removing alkaline solution.

Abstract: Disclosed is a mineral base oil including 85 to 92 wt % of a paraffinic hydrocarbon and 8 to 15 wt % of a naphthenic hydrocarbon and having a Noack volatility of 10 to 12 wt % and a viscosity index of 132 to 142.

Abstract: A traction fluid comprising a blend of 2,3-dicyclohexyl-2,3-dimethylbutane (HAD) and 2,3-dicyclohexyl-2,3-dimethylbutane (iso-HAD) is found to have a lower viscosity at low temperatures when compared to a traction fluid having only HAD or only iso-HAD as a base fluid with no compromise to traction coefficient. The traction fluid may comprise additives. The traction fluid usually comprises HAD:isoHAD between about 8:1 to about 1:3. Further, the HAD:iso-HAD traction fluid blend is produced by a method of simultaneous co-production of hydrogenated HAD and hydrogenated iso-HAD from an alpha styrene dimer and an iso-HAD precursor with a yield of about 90% in a method that does not require a purification step.

Abstract: Polymers used as rolling lubricating agents, to compositions including said polymers, and to alkali metal films including the polymers or compositions on the surface(s) thereof. The use of said polymers and compositions is also described for strip-rolling alkali metals or alloys thereof in order to obtain thin films. Methods for producing said thin films, which are suitable for use in electrochemical cells, are also described. An improved lubricant according to formula I, which, for example, achieves enhanced conductivity, and/or enables the production of electrochemical cells having an improved life span in cycles.

Abstract: A method of remediating pesticides from an agricultural oil includes mixing a reaction solvent, a reducing agent, and an agricultural oil into a reaction mixture in a reaction vessel, controlling the temperature of the reaction mixture, producing a pre-neutralization mixture including a separation solvent, transferring the pre-neutralization mixture into a neutralization reactor that contains a neutralization agent, mixing the pre-neutralization mixture with the neutralization agent and allowing separation into an aqueous layer and a separation solvent layer, draining the aqueous layer, and distilling the separation solvent in the separation solvent layer from the remediated agricultural oils.

Abstract: The present invention relates to a fragrant material containing aromatic essential oil for improving the retention of the smell and intensity of aroma of aromatic essential oil, and to a method for preparing the same. The fragrant material having long-lasting fragrance according to an embodiment of the present invention contains aromatic essential oil and porous silica loading the aromatic essential oil and having controlled hydrophilicity or hydrophobicity of a surface thereof, wherein the required hydrophilic-lipophilic balance (rHLB) of the aromatic essential oil and the O/C ratio in the porous silica satisfy 0.9?required hydrophilic-lipophilic balance (rHLB) of aromatic essential oil/(O/C ratio of porous silica)?2.9.

Abstract: A process for production fatty acids comprising: adjusting pH of a saponifiable material to a value above pH 7 by adding alkaline material; saponifying all saponifiable material by passing alternate electrical current through the material, wherein the said step of saponification occurs in a saponification reactor; acidulating the saponified material by adding acid to pH value below 4; isolating obtained free fatty acids from aqueous layer.

Abstract: Provided are methods, processes and systems for treating a soapstock. In alternative embodiments, provided are systems and methods for treating a soapstock to generate free fatty acids and/or fatty acid derivatives, e.g. fatty acid alkyl esters. In alternative embodiments, provided are systems and methods for realizing the full fatty acid yield of a soapstock by first converting substantially all of the saponifiable material in a soapstock to salts of fatty acids (soaps) and acidulating the soaps to generate free fatty acids and/or fatty acid derivatives, e.g. fatty acid alkyl esters, wherein the soapstock comprises soaps and saponifiable lipids, e.g. glycerides and/or phospholipids, and the generating of free fatty acids and/or fatty acid is achieved.

Abstract: An all natural multipurpose cleaner composition includes: distilled water; lauryl glucoside (AG-60); aloe vera; vitamin E; ethyl alcohol (food grade); a preservative; and citric acid. Further embodiments of the composition include: tea tree oil; oregano; and a scent. The scent may include any of the following: lemon grass; cinnamon; sweet orange; rosemary; lemon and lime; pine; citronella; peppermint; eucalyptus; lavender; tea tree oil; and eucalyptus citriodora.

Abstract: A cleaning product having a spray dispenser and a cleaning composition suitable for spraying and foaming, the composition is housed in the spray dispenser and the composition includes: i) from about 5 to about 15% by weight of the composition of a surfactant system; and ii) from about 0.1 to about 15% by weight of the composition of an specific ester.

Abstract: The present invention relates to compositions such as cleaning compositions comprising enzymes. The invention further relates, use of compositions comprising such enzymes in cleaning processes.

Abstract: The present invention relates to compositions such as cleaning compositions comprising a mix of enzymes. The invention further relates, use of compositions comprising such enzymes in cleaning processes.

Abstract: Cleaning compositions and methods of cleaning comprising endo-?-1,3-glucanase enzyme and surfactant. The weight ratio of surfactant to active endo-?-1,3-glucanase enzyme protein is at least about 1000:1. The compositions and methods are particularly for cleaning cotton fabrics.

Abstract: A method of treating cotton comprising: (i) contacting cotton with an aqueous wash liquor comprising water and an endo-?-1,3-glucanase enzyme; (ii) optionally rinsing the cotton; and (iii) drying the cotton. The wash liquor may contain surfactant. The cotton may be contacted with the aqueous wash liquor with agitation. The method may be for removal of callose from cotton. The method may be for the manufacture of a cotton-containing fabric having improved whiteness maintenance, soil repellency, reduced malodour, anti-wrinkle benefits, and/or improved drying.

Abstract: Described herein is a liquid enzyme preparation containing component (a): at least one salt according to general formula (I) (R2)3N+—(CH2)nC(R3)(R4)—(O—X)m—O—C(O)—R1 A???(I) wherein n is selected from 1 to 12, m is selected from zero to 50, R1 is selected from the group consisting of methyl, ethyl, —CH(OH)—CH(OH)—COOH, CH(OH)—CH3, (E)-CH?CHCOOH, (Z)—CH?CHCOOH, —C6H5, para-HO—C6H4—, o,p-dihydroxyphenyl, and 3,4,5-triydroxyphenyl, R2 are same or different and selected from C1-C10-alkyl, phenyl, R3 and R4 are same or different and selected from hydrogen and C1-C4-alkyl, X is C2-C4-alkylen, and A? is an inorganic or organic counteranion, component (b): at least one enzyme selected from hydrolases (EC 3), and optionally component (c): at least one compound selected from enzyme stabilizers different from component (a), preservatives, and surfactants.

Abstract: A concentrate aqueous composition for removing Type I and Type III rouge from various stainless-steel items. The liquid composition comprises oxalic acid, a phosphonic acid, and various amines. The use of an amine has been found to provide synergistic results in removal of the noted types of corrosion from various stainless-steel items that include medical instruments, machines, equipment, pipes, tools, mixing and storage vessels, and the like.

Abstract: A cleaning solution, composition, and portable cleaner are provided that is all natural, safe, non-toxic, edible, anti-bacterial, anti-viral, anti-microbial, deodorizing, and further includes a pleasant fragrance and taste. In addition, the cleaning composition can be free from sugar, alcohol, surfactants, and other harmful or harsh ingredients. In addition, the cleaning composition allows for quick cleaning of any type of object that may be placed in the mouth, such as pacifiers, toys, candy, eating utensils, musical instruments, and mouth-pieces, among others.

Abstract: This disclosure relates to microbiological culture plates configured for providing hydrologic stability to an inoculant/sample placed upon solid media held within the plate. A culture plate includes a base section and a lid section shaped to fit upon and enclose the base section. The lid section includes an extension extending downward from an upper surface, and a cover attached to the lower end of the extension. The cover is configured so that when the lid section is placed upon the base section, the cover contacts the inoculant/sample placed upon the solid media in the base section.

Abstract: The present disclosure pertains to methods of growing bacterial host cells in a low-density polyethylene (LDPE) bag to produce plasmid DNA or express recombinant protein. The LDPE bag is filled with media, an antibiotic, and host bacterial cells that have been transformed with plasmid DNA encoding a protein of interest. The LDPE bag is sealable to the external environment and incubated at a growth temperature until a desired concentration of bacteria is achieved. When plasmid DNA is desired, host cells are harvested and plasmid DNA is separated from host cell components. When recombinant protein is desired, expression is induced while host cells are in the LDPE bag, followed by the harvest and separation of the recombinant protein. The LDPE bags are sterile and conducive to bacterial growth equal to or greater than that afforded by conventional shake flasks under similar growth conditions.

Abstract: The invention relates to a cell culture container that inhibits adhesion of cells, a method for manufacturing the same and a method for producing cell aggregates using the same. The cell culture container has a coating film containing a copolymer having a recurring unit which contains a group represented by formula (a) and a recurring unit which contains a group represented by formula (b): wherein Ua1, Ua2, Ub1, Ub2 and Ub3 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and An? represents an anion selected from the group consisting of a halide ion, an inorganic acid ion, a hydroxide ion and an isothiocyanate ion, on at least a part of a surface of the container, and the volume of the container is 20 ?L or less.

Abstract: A lattice structure for culturing cells in a bioreactor is effective for culturing high density cells and maintaining cell type homogeneity. The lattice structure includes a plurality of channels forming a set of channels, each of the plurality of channels extending between a first channel pore surface and a second channel pore surface and each of the plurality of channels having a first channel pore and a second channel pore altogether forming a plurality of channel pores on each of the first channel pore surface and the second channel pore surface, wherein each of the channel pores has an area of between about 0.01 mm2 to about 1 mm2, and wherein the lattice structure is made of a biocompatible rigid material having a Young's modulus value of at least 0.5 GPa.

Abstract: Human labor and time can be reduced, and a high-accuracy numerical model can be created. Provided is a pseudo sample creation method including mixing a pre-culture liquid medium that is not used to culture a sample, a post-culture liquid medium that is used to culture the sample, and an additive containing at least one of saccharides, an amino acid, a metabolite, and protein with one another to create a pseudo sample. In addition, provided is a numerical model creation system including: a spectrometer that acquires spectral data of a pseudo sample; a reference value measuring device that measures a reference value of the pseudo sample; and an analyzer that creates a numerical model representing a correlation between the spectral data and the reference value based on the spectral data and the reference values obtained from a plurality of pseudo samples.

Abstract: A polymeric substrate is contacted with a process gas and radio frequency electrical power is introduced in the process gas, forming a treated contact surface that has improved cell recovery compared to an untreated contact surface. The process gas optionally can be nitrogen gas, oxygen gas, or a gas that contains nitrogen atoms, oxygen atoms, or a combination of nitrogen and oxygen atoms. The process optionally improves cell recovery of a chicken embryo cell culture from the treated contact surface.

Abstract: An optofluidic photoreactor including an optical waveguide having an input, characterized by an evanescent optical field confined along an outer surface of the optical waveguide produced by radiation propagating in the optical waveguide, means for inputting light to the input of the optical waveguide, and a photoactive material disposed substantially only within the evanescent field. A method for optically activating a photoactive material in an optofluidic photoreactor to convert carbon dioxide and water into other molecules that may be useful as a fuel or a chemical feedstock.

Abstract: The invention relates to a device for examining a medium (100) inside a bioreactor (200; 201), comprising a sample-taking module (20) for taking a sample of the medium (100). The sample-taking module (20) comprises an uptake region (10; 10a; 10b) that can be arranged to make contact with the medium (100) inside the bioreactor (200; 201). At least two different membranes (15, 16) are positioned on the uptake region (10; 10a; 10b) of said sample-taking module (20), for the purpose of taking a sample of the medium (100).

Abstract: A dynamic incubator system and method for mammalian cells. The dynamic incubator system includes an incubator and a programming device communicatively coupled to the incubator. The programming device includes a memory, one or more processors, a display, and an input mechanism. The programming device is adapted to enable at least one preset temperature and gas concentration sequence to be one or more of designed for the interior of the incubator, saved to the memory of the programming device and/or selected for implementation within the interior of the incubator. The at least one temperature and gas concentration sequence includes programmed changes to the temperature and a CO2 gas concentration. A purging mechanism is coupled to the incubator and releases a portion of a gas concentration to enable rapid changes in the temperature and gas concentration in the incubator.

Abstract: Embodiments described herein generally relate to methods and systems for customizing protocols for use with a cell expansion system. Through a user interface, a user may create a custom task for loading, growing and/or harvesting cells. A custom task may comprise one or more steps, in which a user may add or omit steps, as desired. Data may be entered for settings associated with a custom task, in which embodiments provide for such data to be entered each time the custom task is performed. In another embodiment, the settings for a custom task may be configured, in which such settings may be stored and retrieved upon selection of the custom task. Customization and configuration of a custom task may occur using a diagram view of the cell expansion system, in which process settings are associated with graphical user interface elements.

Abstract: The present invention relates to methods for performing cell culture by using a solid cell culture media form prepared by a 3D printing process.

Abstract: A dissolvable foam scaffold for cell culture is provided herein. The dissolvable foam scaffold includes an ionotropically crosslinked polygalacturonic acid compound selected from at least one of: pectic acid; partially esterified pectic acid, partially amidated pectic acid and salts thereof, and at least one first water-soluble polymer having surface activity.

Abstract: A method for improving quality of a mammalian semen sample includes providing an insoluble carrier carrying at least one ligand that binds to an indicator of impaired sperm membrane integrity and/or motility, or the insoluble carrier carrying at least one molecule capable of binding to the at least one ligand, and contacting the semen sample and the insoluble carrier to provide a bound sperm cell population and an unbound sperm cell population, The insoluble carrier may be a plurality of beads each carrying the at least one ligand or the at least one molecule. The indicator may be associated with one or both of impaired sperm acrosome and impaired sperm plasma membrane integrity. The ligand may be an antibody. The molecule may be one of streptavidin, protein A, and an anti-ligand antibody. Kits for performing the method are provided.

Abstract: The presently disclosed subject matter is to provide a hepatocyte structure, particularly, a hepatocyte structure that mimics nonalcoholic steatohepatitis (NASH). A hepatocyte construct including an aggregate containing hepatocytes and adherent cells that are non-hepatocytes, and wherein the hepatocytes include ballooned hepatocytes is provided. Further, a method for producing a hepatocyte construct, comprising: (i) forming an aggregate by aggregating a cell group comprising hepatocytes and adherent cells that are non-hepatocytes; and (ii) culturing the aggregate is provided.

Abstract: The invention comprises depletive methods of sex-sorting sperm at high event rates, while simultaneously achieving high technical yields.

Abstract: Human pluripotent stem cells are differentiated in vitro into forebrain subdomain structures, which are then fused to generate an integrated system for use in analysis, screening programs, and the like.

Abstract: The present invention refers to an in vitro method for producing a flap of genetically modified cells on fibrin substrate and to the flap so obtained.

Abstract: The present invention relates to compositions and methods for using a minibody. Minibodies described herein comprise a secretion signal, a variable heavy chain fragment, a variable light chain fragment, a constant chain fragment, and a hinge domain between the variable light chain fragment and the constant chain fragment. One aspect includes a nucleic acid encoding a minibody. Other aspects include compositions comprising a minibody and a modified T cell comprising a nucleic acid encoding a minibody. Also included are methods and pharmaceutical compositions comprising the modified T cells for adoptive therapy and treating a condition, such as cancer.

Abstract: The present disclosure provides customizable enucleated erythroid cells or enucleated cells that can be engineered to include, on their surface, a loadable exogenous antigen-presenting polypeptide, wherein the loadable exogenous antigen-presenting polypeptide comprises one or more amino acid substitutions. In some embodiments, the one or more amino acid substitutions stabilize the loadable exogenous antigen-presenting polypeptide on the cell surface. In some embodiments, the loadable exogenous antigen-presenting polypeptide is stabilized on the cell surface in the absence of a polypeptide bound to the loadable exogenous antigen-presenting polypeptide. In some embodiments, the loadable exogenous antigen-presenting polypeptide comprises an exogenous displaceable polypeptide bound to the loadable exogenous antigen-presenting polypeptide. In some embodiments, the loadable exogenous antigen-presenting polypeptide is stabilized on the cell surface upon release of the displaceable polypeptide.

Abstract: The present disclosure provides dry and liquid compositions that include canine platelets and/or canine platelet-derived substances in dried form or rehydrated form from a dried form of canine platelets and/or canine platelet-derived substances, as well as processes for preparing such compositions. The disclosure also provides processes for making the compositions and methods of using the compositions for therapeutic, prophylactic, diagnostic, and research purposes, and kits comprising the compositions.

Abstract: The present invention relates in part to nucleic acids encoding proteins, nucleic acids containing non-canonical nucleotides, therapeutics comprising nucleic acids, methods, kits, and devices for inducing cells to express proteins, methods, kits, and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods for inducing cells to express proteins and for reprogramming and gene-editing cells using RNA are disclosed. Methods for producing cells from patient samples, cells produced using these methods, and therapeutics comprising cells produced using these methods are also disclosed.

Abstract: An object of the present invention is to provide stem cells having excellent proliferation ability and differentiation ability. The present invention relates to stem cells isolated from a neonatal pig, and a preparation method therefor.

Abstract: The inventors discovered that ectodermal mesenchymal stem cells circulating in peripheral blood that are induced by necrotic tissue damage contribute to the regeneration of damaged tissue. On the basis of this discovery, provided are ectodermal mesenchymal stem cells, a method for producing the same, and a screening method for a substance having pluripotent stem cell inductive activity, the screening method using cells induced by necrotic tissue damage in peripheral blood as an index.

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce cells expressing markers characteristic of the pancreatic endocrine lineage that co-express NKX6.1 and insulin and minimal amounts of glucagon.

Abstract: The present disclosure relates to kidney organoids and methods of manufacturing the same. The organoids and methods may be used in a variety of applications such as disease modelling, drug screening, regenerative medicine and 5 scaling up production of kidney cells.

Abstract: A method of generating protein-induced pluripotent stem cells by delivering bacterially expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique, repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells, moving the reprogrammed cells into a feeder-free medium for expansion, and expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells. Also provided are the piPCS cells formed using this method and uses thereof.

Abstract: The present disclosure relates generally to methods and compositions for transferring a genetic circuit from one prokaryotic cell (“donor cell”) to another prokaryotic cell (“recipient cell” or “target cell” which are used interchangeably herein). More specifically, the present disclosure relates to prokaryotic donor cells comprising (i) a genetic circuit of interest and (ii) one or more expressed transcriptional repressor proteins and the use of said donor cells in the efficient transfer of the genetic circuit into a prokaryotic recipient cell. The genetic circuit includes nucleic acid sequences encoding a RNA molecule or protein of interest.

Abstract: Compositions comprising (i) lactate oxidase (LOX) and Catalase (CAT), preferably in a 1:1 molar ratio; or (ii) a fusion polypeptide comprising both LOX and CAT, e.g., LOXCAT, and methods of use thereof for reducing blood lactate levels, increasing blood pyruvate levels, and/or decreasing blood lactate/pyruvate ratio in a subject.

Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

Abstract: The present invention provides enzymes that have been optimized by implementation of Protein Repair One Stop Shop (PROSS), an algorithm that generates protein design(s) for enhanced stability without changing either enzymatic properties or enzyme active site conformation of the respective enzyme. The protein design(s) generated by PROSS introduce mutations to the amino acid sequence of a wild-type protein, resulting in a mutated amino acid sequence that encodes a variant of the wild-type enzyme, i.e., “an enzyme variant”, which has an enhanced stability, core packing, surface polarity and backbone rigidity, a higher functional expression, and/or a combination thereof, compared to the stability core packing, surface polarity and backbone rigidity, functional expression and/or a combination thereof, of the wild-type enzyme.

Abstract: The invention is used in the field of enzyme technology. The invention relates to lipases and to the production thereof, the amino acid sequence of which has been modified in particular with regard to the use thereof in detergents and cleaning agents, all sufficiently similar lipases having a corresponding modification and nucleic acids coding for them. The invention further relates to methods and uses of said lipases and to agents containing them, in particular detergents and cleaning agents.