Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

Abstract: A method of sequencing multiple nucleic acid sequences, including: estimating a concentration of cells in a solution; and depositing a volume of the solution into a well on a well plate using an ejection device, the amount of solution selected to contain no more than a single cell.

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

Abstract: The present disclosure relates to kits for determining the length of a region of tandem repeats in a subject's genome. In some embodiments, the region of tandem repeats in telomeres.

Abstract: Gene expression is measured in a sample of peripheral blood mononuclear cells (PBMCs) obtained from a subject and used to predict organ function recovery. A Function Recovery Potential (FRP) score is assigned to a sample that reflects the measured expression level of the genes identified herein in a direction associated with recovery from organ failure. Treatment of the subject with optimal medical management (OMM) and/or palliative care (PC) is advised when the FRP score is lower than the reference value, and referring the subject for treatment with therapies including—but not limited to—mechanical circulatory support (MCS) surgery, heart transplant (HTx) surgery, or other intervention for advanced heart failure is advised when the FRP score is greater than the reference value. A method for developing an FRP scoring algorithm that predicts a subject's ability to recover from medical intervention for organ failure is also described.

Abstract: The present disclosure relates to measuring gene expression of cells of a blood sample obtained from a mammalian subject suspected of having a tick-borne disease. In particular, the present disclosure provides tools for determining whether a human subject has acute Lyme disease by transcriptome profiling a peripheral blood mononuclear cell sample from the subject.

Abstract: The present invention relates to methods comprising identifying polymorphisms in samples comprising mixtures of genomes, and for determining and/or monitoring the presence or absence of disorders associated with the identified polymorphisms.

Abstract: The present invention provides methods for assessing the likelihood of effectiveness of chemotherapy such as oxaliplatin treatment in colorectal cancer patients as well as their survival prospect by determining the level of miR-133a in the cancer tissue. A kit useful for such methods are also provided.

Abstract: This document provides methods and materials for identifying metastatic malignant skin lesions (e.g., malignant pigmented skin lesions). For example, methods and materials for using quantitative PCR results and correction protocols to reduce the impact of basal keratinocyte contamination on the analysis of test sample results to identify metastatic malignant skin lesions are provided. This document also provides methods and materials for treating skin cancer. For example, methods and materials for identifying a mammal (e.g., a human) having a pre-metastatic skin lesion (e.g., pre-metastatic melanoma) and treating that mammal with pentamidine (4,4?-[pentane-1,5-diylbis(oxy)]dibenzenecarboximidamide) are provided.

Abstract: The present disclosure provides methods, systems, and compositions for parallel processing of nucleic acid samples. Methods and systems of the present disclosure comprise the use of sample-specific barcode sequences, which facilitate the multiplexing of samples, detection of discrete cell populations within a pooled population, and detection of partitions comprising more than one cell.

Abstract: Methods and kits for isolating tumour-derived exosomes by immunocapture with an anti CA-IX antibody, quantifying tumour-related nucleic acid sequences from the isolated exosomes and determining in vitro the presence of a tumour in a subject are provided.

Abstract: Systems and methods are provided for diagnosing cancer by using promoter methylation as an indicator of interest. Key promoter regions of interest are first identified via supervised or unsupervised machine learning applied to the Cancer Genome Atlas via a silico predictive tool. After this, a specially-designed assay is used to detect the presence of these hyper-methylated regions of interest and provide a quantitative, fluorescent readout in order to generate clinical insight. In addition, special advances in material science and microfluidics are used to enhance the sensitivity and specificity of the assay. The workflow is then completed via integration into a smartphone application that provides the necessary data and helps streamline doctor-patient communication.

Abstract: The invention provides methods of detecting residual disease, such as cancer, in a subject. The methods entail amplifying from a sample obtained from the subject one or more nucleic acids having a passenger mutation that contains or results from fusion, such as a translocation, inversion, or deletion.

Abstract: The present invention relates to compositions and methods for diagnosing, prognosing, monitoring, and treating a patient with prostate cancer. In particular, the invention relates to the use of miRNA and snoRNA as expression signatures for identifying a clinically significant prostate cancer.

Abstract: Gene expression profiling in multiple myeloma patients identifies genes that distinguish between patients with subsequent early death or long survival after treatment. Poor survival is linked to over-expression of genes such as ASPM, OPN3 and CKS1B which are located in chromosome 1q. Given the frequent amplification of 1q in many cancers, it is possible that these genes can be used as powerful prognostic markers and therapeutic targets for multiple myeloma and other cancer.

Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.

Abstract: The invention features methods, systems, and panels for rapid detection of Candida species (e.g., Candida auris, Candida lusitaniae, Candida haemulonii, Candida duobushaemulonii, and Candida pseudohaemulonii) in biological samples (e.g., whole blood) and environmental samples (e.g., environmental swabs, e.g., surface swabs), and for diagnosis and monitoring of diseases, including Candidiasis and sepsis.

Abstract: Methods and compositions to impute or predict genotype, haplotype, molecular phenotype, agronomic phenotypes, and/or coancestry are provided. Methods and compositions provided include using latent space to generate latent space representations or latent vectors that are independent of underlying genotypic or phenotypic data. The methods may include generating a universal latent space representation by encoding discrete or continuous variables derived from genotypic or phenotypic data into latent vectors through a machine learning-based encoder framework. Provided herein are universal methods of parametrically representing genotypic or phenotypic data obtained from one or more populations or sample sets to impute or predict a genotype or phenotype of interest.

Abstract: A method of risk stratification for contagious disease is provided. The method performs a reverse transcriptase polymerase chain reaction (RT-PCR) test on a patient to determine the presence of a viral infection. The method next performs tests for the presence of antibody Immunoglobulin M (IgM) and Immunoglobulin G (IgG) assays. The method assigns the patient a level of readiness to return to society corresponding to the combination of the results of the qualitative RT-PCR, IgM, and IgG tests and quantitative IgG+ antibody testing. The levels of readiness to return to society may be verified by a QR code on a mobile device.

Abstract: The invention belongs to the field of microbes and specifically relates to a myroides odoratimimus biocontrol strain for efficiently degrading aflatoxin and an application thereof. A myroides odoratimimus biocontrol strain 3J2MO is preserved at the China Center for Type Culture Collection (referred to as CCTCC) on Jun. 13, 2017 with preservation number CCTCC No. M 2017329. The myroides odoratimimus biocontrol strain of the invention can effectively degrade aflatoxin and may be configured to degrade aflatoxin and treat aflatoxin pollution of food crops.

Abstract: Provided is a method comprising (a) providing a hydrolysis composition of at least 20 wt % of sugar equivalents, wherein the hydrolysis composition comprises a first oligosaccharide, water, optionally a soluble aromatic compound, (b) contacting the hydrolysis composition with a catalyst in a first reactor to hydrolyze at least a portion of the first oligosaccharide to form a first product composition comprising a first monosaccharide and a second oligosaccharide, (c) separating the first monosaccharide from the first product composition to form a second product composition comprising the second oligosaccharide, wherein at least a portion of the second oligosaccharide is a reversion sugar, and (d) converting via a further hydrolysis step at least a portion of the second oligosaccharide to form a third product composition comprising a second monosaccharide.

Abstract: A method for tanning an animal skin may include: (a) putting the animal skin in contact with a first tanning bath including at least one dialdehyde of general formula O?CH—(CH)n—HC?O, wherein n is 0 or an integer number greater than or equal to 1 and less than or equal to 8, and wherein the first tanning bath has a pH greater than or equal to 1 and less than or equal to 5; (b) bringing the pH of the first tanning bath to a pH greater than 5 and less than 8; and/or (c) repeating one or more times the steps (a) and (b) on the animal skin coming from the step (b) using a second or further tanning bath. The first, second, and further tanning baths may be substantially free of surfactants.

Abstract: Disclosed herein is a reduced iron production method enabling heat to be input well into reduced-iron raw materials on a hearth covering material in a reduction-melting furnace to improve the efficiency of treatment thereof. The reduced-iron raw materials are set on the hearth covering material through their falls and reduced on the hearth covering material. The hearth covering material is constituted by carbon materials each having a particle diameter of 5 mm or less. At least 7 mass % of the carbon materials have respective particle diameters each being 0.1 mm or less. This restrains the reduced-iron raw materials from embedment into the hearth covering material.

Abstract: The invention relates to the use of a Q&P steel for production of a formed component (2) for high-wear applications, wherein the Q&P steel has a hardness of at least 230 HB, especially at least 300 HB, preferably at least 370 HB, and a bending angle ? of at least 60°, especially at least 75°, preferably at least 85°, determined to VDA238-100, and/or a bending ratio of r/t<2.5, especially r/t<2.0, preferably r/t<1.5, where t corresponds to the material thickness of the steel and r to the (inner) bending radius of the steel.

Abstract: The present invention relates to tool steels which present an extremely high conductivity while maintaining high levels of mechanical properties the manufacturing process thereof. Tool steels of the present invention are able to undergo low temperature hardening treatments with good homogeneity of the microstructure and can be obtained at low cost.

Abstract: A method of making stainless steel includes smelting metal material and atomizing and powdering the smelted metal material to obtain a first alloy powder, detecting a mass fraction of manganese in the first alloy powder, heating and kneading the first alloy powder with plastic, and granulating, injection molding, and sintering the first alloy powder kneaded with plastic to obtain stainless steel. If the mass fraction of manganese in the first alloy powder is less than 12%, before heating and kneading with plastic, a predetermined amount of manganese-containing material is added to the first alloy powder to obtain a second alloy powder having a mass fraction of manganese of 12-15%.

Abstract: A method for manufacturing a rail includes casting a steel to obtain a semi-product. The steel has a composition comprising 0.20%?C?0.60%, 1.0%?Si?2.0%, 0.60%?Mn?1.60% and 0.5?Cr?2.2%, optionally 0.01%?Mo?0.3%, 0.01%?V?0.30%; the remainder being Fe and impurities. The method also includes hot rolling the semi-product into a hot rolled semi-product having the shape of the rail and comprising a head, with a final rolling temperature TFRT higher than Ar3; and cooling the head to a cooling stop temperature TCS between 200° C. and 520° C. The temperature of the head over time is comprised between a upper boundary having the coordinates defined by A1 (0 second, 780° C.), B1 (50 seconds, 600° C.), and C1 (110 seconds, 520° C.) and a lower boundary having the coordinates defined by A2 (0 second, 675° C.), B2 (50 seconds, 510° C.), and C2 (110 seconds, 300° C.). The method also includes maintaining the head in a temperature range comprised between 300° C. and 520° C.

Abstract: There is provided a high-strength galvanized steel sheet excellent in the external appearance of plating and the hydrogen brittleness resistance, and has a high yield ratio suitable for building materials and automotive collision-resistant parts, and a method for manufacturing the same. Provided is a high-strength galvanized steel sheet including a specific component composition and a specific steel structure, the amount of diffusible hydrogen in the steel being 0.20 mass ppm or less; and a galvanizing layer provided on a surface of the steel sheet, having a content amount of Fe of 8 to 15% in mass %, and an attachment amount of plating per one surface of 20 to 120 g/m2, wherein the amount of Mn oxides contained in the galvanizing layer is 0.050 g/m2 or less, and a yield strength is 700 MPa or more and a yield strength ratio is 65% or more and less than 85%.

Abstract: The present invention relates to a device for carrying component parts to be temperature-controlled, in particular coiled metal strips or metal wires, in a temperature-control device. The carrier device has a base body and a carrier element, to which a component part is attachable, wherein the carrier element is detachably attached to the base body. The base body has a transport coupling, which is configured such that the transport coupling is detachably fixable to a handling system for handling the device.

Abstract: An apparatus and a method for rapidly heating cold-rolled strip steel (10). The apparatus for rapidly heating cold-rolled strip steel (10) comprises a heating zone, a soaking zone, and a cooling zone, and the heating zone is sequentially divided into a first heating section (1), a second heating section (2), a third heating section (3), and a fourth heating section (4) along a moving direction of the strip steel (10) to be heated, the first heating section (1) and the fourth heating section (4) being radiant heating sections, and the second heating section (2) and the third heating section (3) being inductive heating sections. The method for rapidly heating cold-rolled strip steel (10) uses the apparatus for rapidly heating cold-rolled strip steel (10) to heat the strip steel (10).

Abstract: A “modified” meta-stable # titanium alloy that, apart from carbon content, corresponds to the composition range for standard Beta-C titanium alloy. The modified alloy comprises vanadium, chromium, molybdenum, zirconium, aluminium, with maxima for oxygen, iron, nitrogen, hydrogen, yttrium, and other elements (apart from carbon and titanium), with a balance (apart from carbon) of titanium. The modified alloy has carbon present at a stable total carbon level sufficiently in excess of 0.05 wt. % achieving an improvement in the mechanical properties of UTS, DSS and fatigue strength in threaded regions, relative to standard Beta-C alloy with a specified carbon level below 0.05 wt. %, with a maximum carbon content controlled so as to preclude carbide formation having a detrimental effect on the level of fatigue strength.

Abstract: Provided herein are new aluminum alloy products and methods of making these alloys. The aluminum alloy products are age-hardenable, display high strength and formability, and allow for the use of recycled scrap. The aluminum alloys can serve as the core in a clad aluminum alloy product. The alloy products can be used in a variety of applications, including automotive, transportation, and electronics applications.

Abstract: A cemented carbide includes second hard phase grains, wherein the second hard phase grains includes a core portion, and in a case where a total of 70 unit regions that are each constituted of a square having each side of 8?m are provided by successively arranging 7 unit regions in a longitudinal direction and 10 unit regions in a lateral direction in an electron microscope image of any cross section of the cemented carbide captured at a magnification of 1500×, where the total number of core portions in the total of 70 unit regions is calculated, and where a percentage of the number of core portions in each of the unit regions with respect to the total number of core portions is calculated, the number of unit regions in which the percentage is less than 0.43% or more than 2.43% is less than or equal to 10.

Abstract: A solar tower system is disclosed in which the heat transfer media is a molten salt at a temperature greater than 650° C. The components that carry or hold the molten salt are made from commercially available alloys made by Haynes International and sold under the designations HR-120 alloy, 230® alloy and 233™ alloy whose compositions are described herein. The molten salt preferably is MgCl2—KCl.

Abstract: A Ni steel for low temperature according to an aspect of the present invention has a chemical composition within a predetermined range, in which a metallographic structure of a thickness middle portion contains 2.0 vol % to 20.0 vol % of an austenite phase, an average grain size of prior austenite grains is 3.0 ?m to 12.0 ?m, an average aspect ratio of the prior austenite grains is 2.6 to 10.0, a plate thickness is 4.5 mm to 20 mm, a yield stress at room temperature is 590 MPa to 710 MPa, and a tensile strength at the room temperature is 690 MPa to 810 MPa, when the plate thickness is more than 16 mm, the Ni steel contains Ni: 11.5% or more, and when the plate thickness is 16 mm or less and the Ni steel contains Ni: less than 11.5%, the average grain size of the prior austenite grains is 6.0 ?m or less.

Abstract: Provided is a soft magnetic alloy comprising a composition formula (Fe(1?(?+?))X1?X2?)(1?(a+b+c+d+e+f))MaBbPcSidCeZnf. X1 denotes at least one selected from Co and Ni; X2 denotes at least one selected from Cu, Mg, Al, Mn, Ag, Sn, Bi, O, N, S, and rare earth elements; M denotes at least one selected from Ti, V, Cr, Zr, Nb, Mo, Hf, Ta, and W; 0.080?b?0.150, 0?c?0.060, 0?d?0.060, 0?e?0.030, 0.0030?f?0.080, 0.0030?a+f?0.080, b+c?0.100, ??0, ??0, and 0??+??0.50 are satisfied; and the soft magnetic alloy has Fe-based nanocrystals with a bcc structure.

Abstract: Nickel-cobalt materials, methods of forming a nickel-cobalt material, and methods of thermally stabilizing a nickel-cobalt material are provided. A nickel-cobalt material may include a metal matrix composite with amorphous regions and crystalline regions substantially encompassed by a nanocrystalline grain structure with a grain size distribution of about 50 nanometers to about 800 nanometers, and the nanocrystalline grain structure may include widespread intragranular twinning. The metal matrix composite may have a chemical makeup that includes nickel, cobalt, and a dopant such as phosphorus and/or boron. A nickel-cobalt material may be heat treated within a first temperature zone below the onset temperature for grain growth and then within a second temperature zone above the onset temperature for grain growth in the material. Chemical composition and heat treatment may yield a thermally stabilized nickel-cobalt material.

Abstract: A deposition mask includes an effective part in which a plurality of openings are provided, and an outer frame part surrounding the effective part. The effective part includes an outer peripheral area that is adjacent to the outer frame part, and a central area which is surrounded by the outer peripheral area and has a thickness larger than a thickness of the outer peripheral area.

Abstract: A deposition mask apparatus includes: a deposition mask having a plurality of mask through-holes extending from a first surface which is positioned closer to the substrate, to reach a second surface which is positioned opposite to the first surface; and a frame provided with an opening which is overlapped with a part of the deposition mask, the frame supporting the deposition mask.

Abstract: Metallic layers can be selectively deposited on one surface of a substrate relative to a second surface of the substrate. In some embodiments, the metallic layers are selectively deposited on a first metallic surface relative to a second surface comprising silicon. In some embodiments the reaction chamber in which the selective deposition occurs may optionally be passivated prior to carrying out the selective deposition process. In some embodiments selectivity of above about 50% or even about 90% is achieved.

Abstract: A device for coating an interior surface of a housing defining a volume comprising a plurality of reactant gas sources including reactant gases for one or more surface coating processes; first and second closures to sealingly engage with an inlet and outlet of the volume of the housing to provide an enclosed volume; a delivery line fluidically coupled to the first closure and the plurality of reactant gas sources to deliver the reactant gases to the enclosed volume; and an output line fluidically coupled to the second closure to remove one or more reactant gases, byproduct gases, or both from the enclosed volume. A method for coating an interior surface of a housing is also provided.

Abstract: A laminated film including: a substrate; and a thin film layer stacked on at least one surface of the substrate, the thin film layer containing silicon atoms, oxygen atoms, and carbon atoms, the thin film layer having at least three extrema, a difference between a maximum value of local maxima and a minimum value of the local maxima of 14 at % or less, and a maximum value of 23 at % to 33 at % in a carbon distribution curve that represents a relationship between a thickness-wise distance in the thin film layer from a surface of the thin film layer and a proportion of the number of carbon atoms (atomic proportion of carbon) to a total number of silicon atoms, oxygen atoms, and carbon atoms contained in the thin film layer at a point positioned away from the surface by the distance, and the thin film layer including at least one discontinuous region that satisfies a relationship of formulae (1) to (3), 3 at %?a?b??(1) 3 at %?b?c??(2) 0.5<(a?c)/dx??(3).

Abstract: An apparatus for forming a predetermined film on a substrate, includes a processing container into which a film-forming raw material gas is introduced, a stage provided inside the processing container, and a housing body provided below the processing container and communicating with an exhaust port provided in a bottom portion of the processing container. The housing body includes a housing section that houses a support member of the stage, and a manifold section opened toward the housing section while being in communication with an exhaust device. An exhaust space is formed at a side of a lower surface of the stage. Atmosphere above the stage flows into the exhaust space along a peripheral portion of the stage. The housing section is smaller in horizontal cross-sectional area than the exhaust space. An inlet of the housing section is smaller in cross-sectional area than an outlet of the housing section.

Abstract: A gas ejector of a gas supply apparatus includes a nozzle portion. The opening of a first-stage restricting cylinder constituting the nozzle portion has a circular cross-sectional shape with a diameter r1. A second-stage restricting cylinder is continuously formed with the first-stage restricting cylinder along a Z direction. The opening of the second-stage restricting cylinder has a circular cross-sectional shape with a diameter r2, and supplies a source gas supplied from the first-stage restricting cylinder to a low-vacuum processing chamber below. At this time, the diameter r2 is set to satisfy “r2>r1”.

Abstract: There is provided a technique that includes: a substrate transfer chamber; a pod transfer chamber; a plurality of reinforcement structures installed along a wall of a housing constituting the substrate transfer chamber and forming a plurality of first confinement spaces between the reinforcement structures and the wall; a communication hole installed at each of the plurality of reinforcement structures so that a space in the housing and each of the plurality of first confinement spaces communicate with each other; a collecting pipe having the plurality of reinforcement structures connected in the housing and including a second confinement space communicating with the plurality of first confinement spaces; and a pressure regulator connected to the collecting pipe, and configured to perform a regulation so that a relationship of pressure is satisfied.

Abstract: Provided herein are methods, systems, and devices incorporating use of materials to store, ship, and deliver process gases to micro-electronics fabrication processes and other critical process applications.