Patents Issued in November 12, 2020
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Publication number: 20200354726Abstract: The present disclosure relates to the design of a recombinant vector for production and separation/purification of a carbonic anhydrase in a plant, a method of separating and purifying a recombinant protein including a carbonic anhydrase produced in a plant by using the capacity of a CBM3 domain of the recombinant protein to bind to cellulose beads, and a method of inducing the hydration reaction of carbon dioxide using a carbonic anhydrase in a state of being firmly immobilized onto the surfaces of cellulose beads by CBM3.Type: ApplicationFiled: March 20, 2020Publication date: November 12, 2020Applicant: BIOCOMPANION INC.Inventors: In Hwan HWANG, Md Abdur RAZZAK, Bong Ryul RYU, Dong Wook LEE, Jun Ho LEE, Madhu KUMARI
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Publication number: 20200354727Abstract: The present invention relates to modified bacterial spores, and particularly, although not exclusively, to modified bacterial spores which comprise one or more heterologous genes, but without the introduction of antibiotic resistance genes. The invention extends to methods for producing such modified bacterial spores, vectors and kits for introducing heterologous genes in a spore.Type: ApplicationFiled: November 1, 2018Publication date: November 12, 2020Inventor: Simon CUTTING
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Publication number: 20200354728Abstract: The disclosure provides methods and apparatus for producing 3-hydroxypropionic acid or a salt thereof, for removing 3-hydroxypropionic acid from aqueous solution (e.g., aqueous broth), and for using it to make various chemicals.Type: ApplicationFiled: April 24, 2020Publication date: November 12, 2020Inventors: Jeung Yeop Shim, Ki Soo Park, Ashok Somasundar, Sung Hoon Park
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Publication number: 20200354729Abstract: Provided are base editors containing a cytidine and a catalytically inactive version of Lachnospiraceae bacterium Cpf1 (LbCpf1). The new base editors have greatly improved editing efficiency and fidelity as compared to Cas9-based base editors, and have different editing windows.Type: ApplicationFiled: August 28, 2018Publication date: November 12, 2020Inventors: Jia Chen, Xingxu Huang, Li Yang, Bei Yang, Xiaosa Li, Ying Wang, Yajing Liu
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Publication number: 20200354730Abstract: Systems and methods for yeast vaccines are presented that allow for selection of tumor neoepitopes that are then used to generate a recombinant polytope for yeast expression with enhanced immunogenicity.Type: ApplicationFiled: November 11, 2018Publication date: November 12, 2020Inventors: Kayvan Niazi, Thomas King
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Publication number: 20200354731Abstract: Isolated polynucleotides comprising a transcriptional unit are disclosed, the transcriptional unit comprising: (i) a nucleic acid sequence that encodes a secreted protein of interest; (ii) an endoplasmic reticulum (ER) targeting sequence as set forth in SEQ ID NO: 2, said ER targeting sequence being heterologous to said secreted protein of interest; (iii) a promoter; and (iv) a transcription termination site.Type: ApplicationFiled: July 28, 2020Publication date: November 12, 2020Applicant: Yeda Research and Development Co. Ltd.Inventors: Jeffrey E. GERST, Lisha Qiu Jin LIM, Yitzhak PILPEL, Tsviya OLENDER, Dvir DAHARY, Osnat COHEN-ZONTAG
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Publication number: 20200354732Abstract: Methods and systems for delivering biological materials such as nucleic acids and/or proteins to the interior of a plant cell are provided. Such methods and systems include those where biological material is non-covalently complexed with a magnetic particle and accelerated multiple times toward a plant cell.Type: ApplicationFiled: April 22, 2020Publication date: November 12, 2020Inventors: John Paul Issa, Grethel Yanet Busot
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Publication number: 20200354733Abstract: Methods and compositions of improving plant yield by introducing into a plant the K-domain of a MADS box gene are disclosed. The expression of the K-domain provides plants with altered flower development, plant size and leaf development.Type: ApplicationFiled: November 4, 2019Publication date: November 12, 2020Inventors: Guo-qing Song, Aaron Walworth, Dongyan Zhao
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Publication number: 20200354734Abstract: The present invention relates to improved methods for precision genome editing (GE), preferably in eukaryotic cells, and particularly to methods for GE in cells with specifically altered expression of Polymerase theta and altered characteristics of at least one further enzyme involved in a non-homologous end-joining (NHEJ) DNA repair pathway. Further provided are cellular systems and tools related to the methods provided. Specifically, methods are provided, wherein Polymerase theta and NHEJ blockage and/or GE are performed in a transient way so that the endogenous Polymerase theta and cellular NHEJ machinery is easily reactivated after a targeted edit, and/or without permanent integration of certain editing tools.Type: ApplicationFiled: October 30, 2018Publication date: November 12, 2020Applicant: KWS SAAT SE & Co. KGaAInventors: Markus NIESSEN, Aaron HUMMEL, Erik JONGEDIJK, Zarir VAGHCHHIPAWALA, Fridtjof WELTMEIER, René GLENZ
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Publication number: 20200354735Abstract: The invention relates to genetically modified plants with an altered seed phenotype, in particular increased seed size. The invention relates to a plant that does not produce a functional NGAL2 polypeptide or functional NGAL2 and NGAL3 polypeptides. NGAL2 and NGAL3 are members of the RAV family and comprise a B3 DNA-binding domain and a transcriptional repression motif.Type: ApplicationFiled: July 6, 2020Publication date: November 12, 2020Inventor: YUNHAI LI
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Publication number: 20200354736Abstract: The present invention provides for a genetically modified plant or plant cell comprising a nucleic acid encoding a S-adenosylmethionine hydrolase (AdoMetase) operatively linked to a tissue-specific secondary wall promoter such that there is a specific increased expression of AdoMetase in a secondary cell wall synthesizing tissue when compared secondary cell wall promoter expression compared to corresponding unmodified plant cells.Type: ApplicationFiled: January 17, 2020Publication date: November 12, 2020Applicant: The Regents of the University of CaliforniaInventors: Dominique Loque, Aymerick G. Eudes
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Publication number: 20200354737Abstract: Provided is a technique for highly expressing a target protein in a plant cell by using a glycosylation domain, a recombinant vector comprising a gene encoding a fusion protein of a glycosylation domain and a target protein, a recombinant cell, a transformed plant, and a method of producing a target protein using these.Type: ApplicationFiled: January 17, 2018Publication date: November 12, 2020Applicant: BIOAPPLICATIONS INC.Inventors: In Hwan HWANG, Hyang Ju KANG
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Publication number: 20200354738Abstract: The present invention provides a transgenic soybean comprising event MON87712 that exhibits increased yield. The invention also provides cells, plant parts, seeds, plants, commodity products related to the event, and DNA molecules that are unique to the event and were created by the insertion of transgenic DNA into the genome of a soybean plant. The invention further provides methods for detecting the presence of said soybean event nucleotide sequences in a sample, probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of said soybean event.Type: ApplicationFiled: May 21, 2020Publication date: November 12, 2020Inventors: Robert H. Cole, John A. Korte, John R. LeDeaux, Melissa Compton Spears, Kunsheng Wu
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Publication number: 20200354739Abstract: The present invention relates to methods and compositions for identifying, selecting and/or producing a Disease resistant soybean plant or germplasm using markers, genes and chromosomal intervals derived from Glycine tomentella PI441001, PI441008, PI446958, PI583970, or PI483224. A soybean plant or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided. Disease resistant soybean seeds, plants and germplasms are also provided.Type: ApplicationFiled: November 16, 2018Publication date: November 12, 2020Applicant: Syngenta Participations AGInventors: Qingli Liu, Andrew David Farmer, Xiaoping Tan, Thomas Joseph Curley, Jr., Becky Welsh Breitinger, John Luther Dawson, John Daniel Hipskind, Robert Arthur Dietrich
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Publication number: 20200354740Abstract: The present invention relates to new transporter polypeptides, and genes encoding therefor, which can be used to confer upon a plant resistance to one or more biotrophic fungal pathogens.Type: ApplicationFiled: April 3, 2020Publication date: November 12, 2020Inventors: Evans Lagudah, John Wallace Moore
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Publication number: 20200354741Abstract: Disclosed herein are highly efficient gene-editing systems for editing a voltage-gated sodium channel gene, such as sodium voltage-gated channel alpha subunit 9 (SCN9A) or sodium voltage-gated channel alpha subunit 10 (SCN10A), either in vitro or in vivo. The gene-editing systems disclosed herein comprise RNA-guided DNA endonuclease and specific guide RNAs. Also provided herein are uses of the gene-editing systems to modify the target gene, thereby alleviating pain.Type: ApplicationFiled: April 10, 2020Publication date: November 12, 2020Inventors: Sheena Saayman, Stuart Cahalan, Angela Yen, Sophia Espanola, Sarah Jean Spencer
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Publication number: 20200354742Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.Type: ApplicationFiled: July 24, 2020Publication date: November 12, 2020Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard CollegeInventors: Feng ZHANG, Le Cong, Fei Ran
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Publication number: 20200354743Abstract: Genetically modified compositions, such as adeno-associated viral vectors and primary cells, for treating various conditions and diseases. Disclosed are also modified adeno-associated viruses for the treatment of cancer. Also disclosed are the methods of making and using the genetically modified compositions in treating various diseases, conditions, and cancer.Type: ApplicationFiled: December 18, 2019Publication date: November 12, 2020Inventors: THOMAS HENLEY, MAVIS AGBANDJE-MCKENNA, TILMANN BUERCKSTUEMMER, LYDIA VINEY, MODASSIR CHOUDHRY
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Publication number: 20200354744Abstract: The invention relates to recombinant adeno-associated virus (rAAV) virions for gene therapy, wherein the rAAV virions comprise a novel capsid protein. In particular, the invention relates to the use of such virions in gene therapy for the treatment of an arthritic disease, such as for example rheumatoid arthritis, or symptoms thereof, preferably by intraarticular administration.Type: ApplicationFiled: January 17, 2019Publication date: November 12, 2020Inventors: Jonathan Douglas Finn, Dirk Grimm, Kathleen Börner, Niels Broekstra, Susanne Anna Snoek, Sabine Maria Gertrude Van Der Sanden
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Publication number: 20200354745Abstract: In various aspects, the present disclosure provides polynucleotides encoding a fusion protein, as well as vectors, cells, and compositions comprising the same. In embodiments, the fusion protein includes an insulin signal peptide and an MDA-7/IL-24 protein. Methods of using the polynucleotides, vectors, cells, and compositions, such as in the treatment or prevention of cancer, are also provided.Type: ApplicationFiled: January 23, 2019Publication date: November 12, 2020Inventors: Paul B. Fisher, Mitchell E. Menezes, Praveen Bhoopathi, Swadesh K. Das, Luni Emdad, Devanand Sarkar, Anjan K. Pradhan, Xiang-yang Wang
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Publication number: 20200354746Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. in certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. in some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: ApplicationFiled: March 11, 2020Publication date: November 12, 2020Applicant: Amyris, Inc.Inventors: Andrew HORWITZ, Kristy Michelle HAWKINS, Max SCHUBERT, Wayne SZETO
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Publication number: 20200354747Abstract: The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.Type: ApplicationFiled: July 21, 2020Publication date: November 12, 2020Inventors: Jennifer A. Doudna, Martin Jinek, Krzysztof Chylinski, Emmanuelle Charpentier
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Publication number: 20200354748Abstract: The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together, with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.Type: ApplicationFiled: July 21, 2020Publication date: November 12, 2020Inventors: Jennifer A. Doudna, Martin Jinek, Krzysztof Chylinski, Emmanuelle Charpentier
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Publication number: 20200354749Abstract: The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together, with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.Type: ApplicationFiled: July 21, 2020Publication date: November 12, 2020Inventors: Jennifer A. Doudna, Martin Jinek, Krzysztof Chylinski, Emmanuelle Charpentier
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Publication number: 20200354750Abstract: The present disclosure provides DNA-guided CRISPR systems; polynucleotides comprising DNA, RNA and mixtures thereof for use with CRISPR systems; and methods of use involving such polynucleotides and DNA-guided CRISPR systems.Type: ApplicationFiled: July 28, 2020Publication date: November 12, 2020Inventors: Andrew Paul May, Paul Daniel Donohoue
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Publication number: 20200354751Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.Type: ApplicationFiled: July 30, 2020Publication date: November 12, 2020Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard CollegeInventors: Feng Zhang, Fei Ran
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Publication number: 20200354752Abstract: Engineered Cas9 systems that utilize alternate protospacer adjacent motifs for target DNA binding, nucleic acids encoding the engineered Cas9 systems, and methods of using the engineered Cas9 systems for modifying target chromosomal sequences in eukaryotic cells.Type: ApplicationFiled: July 30, 2020Publication date: November 12, 2020Inventors: Timothy Seebeck, Fuqiang Chen, Gregory D. Davis
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Publication number: 20200354753Abstract: The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.Type: ApplicationFiled: October 25, 2019Publication date: November 12, 2020Inventors: Priti Pharkya, Anthony P. Burgard, Mark J. Burk
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Publication number: 20200354754Abstract: An object of the present invention is to provide a method for preparing ambrein, which can easily and efficiently obtain the ambrein. The object can be solved by a mutated tetraprenyl-?-curcumene cyclase wherein (1) a 4th amino acid residue of a DXDD motif, aspartic acid, is substituted with an amino acid other than aspartic acid, and (2) an amino acid adjacent to the N-terminus of a (A/S/G)RX(H/N)XXP motif is substituted with an amino acid other than tyrosine, or a 4th amino acid of the GXGX(G/A/P) motif is substituted with an amino acid other than leucine.Type: ApplicationFiled: August 31, 2018Publication date: November 12, 2020Applicants: Niigata University, Adeka CorporationInventors: Tsutomu Sato, Tsutomu Hoshino, Toshihiko Takehana, Seiji Koike, Koichi Shigeno
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Publication number: 20200354755Abstract: The present invention relates to a novel microorganism capable of metabolizing various carbon sources at high rates. A novel microorganism according to the present invention was observed to grow at a very high rate in a minimal medium/nutrient medium, etc., compared to microorganisms such as Escherichia coli, and shows resistance at a high initial sugar/salt concentrations as well as being able to produce lycopene and 2,3-butanediol through genetic manipulation. Therefore, the novel microorganism can be used in various production fields of high value-added compounds using microorganisms.Type: ApplicationFiled: May 11, 2018Publication date: November 12, 2020Inventors: Gyoo Yeol Jung, Dong Hun Kwak, Sang Woo Seo, Hyun Gyu Lim
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Publication number: 20200354756Abstract: Described are compositions and methods relating to yeast having a genetic mutation that results in increased alcohol production and tolerance, including increased ethanol production and increased butanol tolerance. Such yeast is well-suited for use in alcohol and butanol production to increase yields.Type: ApplicationFiled: April 3, 2020Publication date: November 12, 2020Inventors: ZHONGQIANG CHEN, STEVEN ADAM KANE, CELIA EMILY GABY PAYEN, GERDA SAXER QUANCE, LUAN TAO
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Publication number: 20200354757Abstract: Controlled coenzyme Q10 (CoQ10) fermentation production processes and methods for controlling the CoQ10 fermentation production processes are provided in the present disclosure. The processes may include growing a microbial culture of bacteria by providing a carbon source and an oxygen source for a predetermined period of time, thereby producing CoQ10-containing bacteria, carbon dioxide, and lactate in the bacterial culture. During various stages of the production process, the concentration of carbon dioxide may be maintained at predetermined levels, respectively. Alternatively or additionally, during various stages of the production process, the concentration of lactate may be maintained at predetermined levels, respectively.Type: ApplicationFiled: October 11, 2019Publication date: November 12, 2020Applicants: INNER MONGOLIA KINGDOMWAY PHARMACEUTICAL CO., LTD., XIAMEN KINGDOMWAY GROUP COMPANYInventors: Guanghuang ZHAN, Yi WU, Biqin CHEN, Zhichun ZHU, Dan LI
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Publication number: 20200354758Abstract: The present invention generally relates to the field of biotechnology as it applies to the production of aryl sulfates using polypeptides or recombinant cells comprising said polypeptides. More particularly, the present invention pertains to polypeptides having aryl sulfotransferase activity, recombinant host cells expressing same and processes for the production of aryl sulfates employing these polypeptides or recombinant host cells.Type: ApplicationFiled: July 30, 2020Publication date: November 12, 2020Inventors: Christian Bille Jendresen, Alex Toftgaard Nielsen
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Publication number: 20200354759Abstract: The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine and treating dopamine-responsive disorders, such as Parkinson's Disease. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4,5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as M. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.Type: ApplicationFiled: July 28, 2020Publication date: November 12, 2020Inventors: Asaph AHARONI, Guy POLTURAK
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Publication number: 20200354760Abstract: The present invention concerns an efficient way to isolate L-fucose from a fermentation broth. The L-fucose contained in the fermentation broth is produced by microbial fermentation (bacterial or yeasts). The inventive process comprises a step of removing biomass from the fermentation broth, a step of subjecting the resulting solution to at least one of a cationic ion exchanger treatment and an anionic ion exchanger treatment and a step of removing salts after the ion exchanger treatment. The process can provide L-fucose in powder form, in granulated form as well as in form of L-fucose crystals.Type: ApplicationFiled: November 15, 2018Publication date: November 12, 2020Inventors: Markus HELFRICH, Stefan JENNEWEIN
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Publication number: 20200354761Abstract: The invention relates to a genetically modified microorganism for making a oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.Type: ApplicationFiled: July 13, 2020Publication date: November 12, 2020Inventors: Margit Pedersen, Manos Papadakis, Peter Becker, Eric Samain, Pauline Peltier-Pain, Katrine Bych, Ted Johanson, Elise Champion, Gyula Dekany
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Publication number: 20200354762Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.Type: ApplicationFiled: April 3, 2020Publication date: November 12, 2020Applicant: Novoyzmes, Inc.Inventors: Brett McBrayer, Tarana Shaghasi, Elena Vlasenko
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Publication number: 20200354763Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.Type: ApplicationFiled: May 19, 2020Publication date: November 12, 2020Applicant: Novozymes A/SInventors: Jeffrey Shasky, Amanda Fischer, Suchindra Maiyuran
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Publication number: 20200354764Abstract: Devices and methods capable of detecting glucose in saliva (FIG. 12). The devices feature a sensor having a substrate containing electrodes and one or more reagents on the electrodes. A detection device is operably coupled with the sensor to detect glucose based on measurement of an electrical parameter when electricity is applied to the electrodes.Type: ApplicationFiled: June 25, 2020Publication date: November 12, 2020Inventors: Jeffrey La Belle, Chi Lin, Jonus Reyna, Amnah Alkhan, Cael Muggeridge, Susan Sheffield
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Publication number: 20200354765Abstract: One or more live substances is cultured at a plurality of test locations of a test vessel. The test locations include a thermochromic material and one or more test substances. A spectral shift in light emanating from the thermochromic material of the test locations is detected. The spectral shift occurs in response to an increase or decrease in energy conversion by the live substance. An effect of the one or more test substances on the live substances is determined based on the detected spectral shift.Type: ApplicationFiled: July 27, 2020Publication date: November 12, 2020Inventors: Joerg Martini, Michael I. Recht, Peter Kiesel
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Publication number: 20200354766Abstract: Method to classify and identify microorganisms that provides to classify and identity microorganisms using the FTIR ATR technique.Type: ApplicationFiled: January 31, 2019Publication date: November 12, 2020Applicant: ALIFAX S.R.L.Inventor: Paolo Galiano
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Publication number: 20200354767Abstract: Methods of measuring multiple enzyme activities in parallel in a sequencing-based assay to characterize enzyme activities in individual mammalian cells. In preferred implementations, the methods involve forming microfluidic droplets containing oligonucleotide functionalized microbeads and single mammalian cells, lysing the cells, and allowing enzyme activity on enzyme substrates present in the oligonucleotides, isolating the individual microbeads, and determining the enzymatic activity to quantitate and evaluate the enzymatic activity or capacity of the cells.Type: ApplicationFiled: November 16, 2018Publication date: November 12, 2020Inventors: Jay HESSELBERTH, Amanda L. Richer
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Publication number: 20200354768Abstract: Biomarkers and methods for identifying, verifying and confirming circulating serum-based microRNAs. The microRNAs (PARKmiRs) can be used to differentiate patient's suffering from Parkinson's disease (PD) from non-PD patients.Type: ApplicationFiled: February 3, 2017Publication date: November 12, 2020Inventors: Simon Geir Moller, Indranil Basak, Ketan Patil, Jan Petter Larsen
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Publication number: 20200354769Abstract: The present invention relates to compositions and methods for preserving and extracting nucleic acids from saliva. The compositions include a chelating agent, a denaturing agent, buffers to maintain the pH of the composition within ranges desirable for DNA and/or RNA. The compositions may also include a reducing agent and/or antimicrobial agent. The invention extends to methods of using the compositions of the invention to preserve and isolate nucleic acids from saliva as well as to containers for the compositions of the invention.Type: ApplicationFiled: March 4, 2020Publication date: November 12, 2020Inventor: H. Chaim Birnboim
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Publication number: 20200354770Abstract: The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.Type: ApplicationFiled: March 18, 2020Publication date: November 12, 2020Inventors: Alexander Azzawi, Ralf Peist
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Publication number: 20200354771Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.Type: ApplicationFiled: April 22, 2020Publication date: November 12, 2020Inventors: Zahra Kamila Belhocine, Josephine Lee, Francesca Meschi, Luz Montesclaros, Katherine Pfeiffer, Andrew D. Price, Jerald Sapida, David Sukovich, Tobias Daniel Wheeler, Yifeng Yin
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Publication number: 20200354772Abstract: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.Type: ApplicationFiled: June 4, 2020Publication date: November 12, 2020Applicant: STOKES BIO LTD.Inventors: Mark DAVIES, Tara DALTON
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Publication number: 20200354773Abstract: The present disclosure provides methods and kits for performing high multiplex PCR using molecular barcodes. The methods disclosed herein separately extend a set of primers (BC primers) that each comprise a molecular barcode and a target-specific sequence, and amplify the resulting extension products using another set of primers (LA primers) that each comprise another target-specific sequence. Such methods avoid barcode resampling and suppress primer dimers in traditional high multiplex PCR.Type: ApplicationFiled: July 28, 2020Publication date: November 12, 2020Inventors: Yexun Wang, Quan Peng
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Publication number: 20200354774Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.Type: ApplicationFiled: July 29, 2020Publication date: November 12, 2020Applicant: President and Fellows of Harvard CollegeInventors: George M. Church, Je-hyuk Lee, Richard C. Terry, Evan R. Daugharthy
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Publication number: 20200354775Abstract: The disclosed invention is related to compositions, kits and methods comprising one or more oligomers targeting 16S rRNA target nucleic acid from Campylobacter species jejuni, coli and/or lari. Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers.Type: ApplicationFiled: July 20, 2020Publication date: November 12, 2020Inventors: Michael R. Reshatoff, JR., Jennifer J. Bungo, Shannon K. Kaplan, James J. Hogan
