Abstract: A set of systems for receiving and processing samples, for capturing, concentrating and purifying target molecules labelled by applying a magnetic field, for exciting the labelling particles so as to generate a light signal and for acquiring and digitally processing the recorded signal to convert it into a qualitative variable that indicates the presence or absence of the target molecule in a test sample by means of an indicator light, a screen or any other digital interface or by means of any other system that makes it possible to view the results obtained. It is possible to conduct a quantitative analysis of the concentration of the target molecule in the analysed sample by comparing the intensity of the recorded signal with previously calibrated reference values.

Abstract: The invention relates to a method and a DNA nanostructure for detecting a target structure. The invention relates in particular to a DNA nanostructure, which, by skillful selection of the shape of the DNA nanostructure and the placement of marker molecules fastened thereon, ensures a preferably linear dependence on the number of marker molecules and measurement signal, independent of the local arrangement of a plurality of such DNA nanostructures. The invention further relates to using said DNA nanostructures and other nano reporters, preferably in combination with adapters specifically binding to target molecules, in a method for the preferably simultaneous quantification of a plurality of target molecules, by means of a multiplex method. The method relates in particular to a single cell analysis.

Abstract: The present invention relates to the rapid and electricity-free, point-of-care, multiplexed detection and quantification of at least one or more nucleic acid sequences from nucleic acids corresponding to a plurality of pathogens or biomarkers using a micropatterned lateral flow device. Rapid and molecular-level sensitive differential diagnosis of a disease condition may be enabled without the need for a delayed laboratory test so that timely treatment can be administered.

Abstract: Disclosed herein are methods, devices and compositions comprising nucleic acid captor molecules with a stem region and a loop region for detecting target nucleic acids.

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

Abstract: The purpose of the present invention is to provide a single cell analysis device in which the improvement of the nucleic acid capturing efficiency and the improvement of the cell capturing efficiency are both achieved and a highly accurate single cell analysis data is thereby obtained. The present invention relates to an improvement of a cell analysis device including a two-dimensional array chip having a plurality of cell capture parts capable of capturing a single cell in each of the capture parts, and nucleic acid capture parts corresponding to the respective cell capture parts, the nucleic acid capture parts being capable of capturing a nucleic acid extracted from the cell captured by the cell capture part.

Abstract: The invention relates to imaging, such as in situ imaging by expansion microscopy, labelling, and analyzing biological samples, such as formalin fixed paraffin embedded (FFPE) cells and tissues, as well as reagents and kits for doing so.

Abstract: Compositions comprising covalently modified and mutated biotin-binding proteins, particularly biotin-binding proteins having a negative charge at physiological pH, are provided. Methods of producing such proteins are also provided, as are methods of immobilizing, sequencing, and making nucleic acids employing such proteins.

Abstract: Provided herein are methods for target gene sequencing and single cell barcoding in conjunction with analysis of gene expression in single cells. In some embodiments, the target gene is an immune molecule, such as an antibody or TCR. In some embodiments, the methods can be used to carry out transcriptome sequencing, e.g., RNA sequencing, to capture transcriptome of single cells paired with full receptor immune receptor sequences such that information about the immune repertoire and transcriptome of a cell can be determined. Also provided are polynucleotide libraries for use in carrying out transcriptome analysis and immune molecule, e.g., antibody or TCR, sequencing.

Abstract: Particular forward and reverse primers may be used to link distant regions of the same large DNA molecule into a smaller DNA molecule. A reverse primer R1 can have a first portion complementary to an ending sequence of region A and can have a second portion having an overlapping sequence. A forward primer F2 can have a first portion complementary to a starting sequence of region B, where the forward primer includes a complementary overlapping sequence (e.g., the same first portion or a second portion) that is complementary to the overlapping sequence. The first portion of F2 may be the entire primer. The smaller DNA molecules can be used to determine haplotypes of regions. Kits including the particular forward and reverse primers are also described.

Abstract: A method of detecting a target polynucleotide sequence in a given nucleic acid analyte characterised by the steps of: a. annealing the analyte to a single-stranded probe oligonucleotide A0 to create a first intermediate product which is at least partially double-stranded and in which the 3? end of A0 forms a double-stranded complex with the analyte target sequence; b. pyrophosphorolysing the first intermediate product with a pyrophosphorolysing enzyme in the 3?-5? direction from the 3? end of A0 to create partially digested strand A1 and the analyte; c. (i) annealing A1 to a single-stranded trigger oligonucleotide B and extending the A1 strand in the 5?-3? direction against B; or (ii) circularising A1 through ligation of its 3? and 5? ends; or (iii) ligating the 3? end of A1 to the 5? end of a ligation probe oligonucleotide C; in each case to create an oligonucleotide A2; d. priming A2 with at least one single-stranded primer oligonucleotide and creating multiple copies of A2, or a region of A2; and e.

Abstract: The invention is directed to a method for determining a monomer sequence of a polymer that is translocated through a nanopore. Monomers of the polymer are labeled with fluorescent labels such that in free solution fluorescent labels of adjacent monomers substantially quench each other and wherein the nanopore constrains fluorescent labels within its bore into a constrained state wherein no detectable fluorescent signal can be generated. By exciting the fluorescent label of each monomer as it exits the nanopore and transitions from a constrained state to a quenched state with an adjacent fluorescent label, a fluorescent signal can be generated by the exiting fluorescent label that allows its monomer to be identified, thereby permitting a monomer sequence to be determined from a sequence of fluorescent signals as the polymer translocates through the nanopore.

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

Abstract: The present invention includes a method for identifying an Alzheimer's disease (AD) patient prior to reaching clinical disease classification, comprising: obtaining a dataset associated with a blood, serum, or plasma sample from the patient, wherein the dataset comprises data representing the level of one or more microRNA biomarkers in the blood, serum, or plasma sample; assessing the dataset for a presence or an increase in an amount of miRNA-455-3p; determining the likelihood that the patient will develop AD patient prior to reaching clinical disease classification by detecting the presence or the increase in miRNA-455-3p to produce a score that is indicative of a likelihood of developing AD, wherein a higher score relative to a healthy control indicates that the patient is likely to have the prognosis for transitioning to classified AD, wherein the healthy control is derived from a non-AD patient with no clinical evidence of AD.

Abstract: Provided herein are methods for prognosing and treating a patient with Parkinson's disease (PD) or Parkinsonian symptoms. The prognosis and appropriate treatments can be determined by correlating the level of gene expression of SKP1a, UBE2K, ALDH1A1, PSMC4, HSPA8 and LAMB2 with a Parkinson's Disease rating scale, thereby prognosing slow or rapid progression of the symptoms or disease.

Abstract: Methods and nucleic acid molecules for detecting chromosomal abnormalities such as aneuploidy. Methods for selecting nucleic acid molecules for use in the methods of the disclosure.

Abstract: Methods of treating a tumor in a subject and methods of determining a treatment regimen for a subject with a tumor are provided herein. In exemplary aspects, the methods comprise measuring the level of expression of immunoglobulin, FCGR2B, a gene listed in Table 4, or a combination thereof. In exemplary aspects, the subject is a subject from which a sample was obtained, wherein the level of immunoglobulin, FCGR2B, a gene listed in Table 4, or a combination thereof, has been measured from the sample. Related kits, computer readable-storage media, systems, and methods implemented by a processor in a computer are further provided.

Abstract: The present invention relates to a method for determining sensitivity to a simultaneous inhibitor against poly ADP ribose polymerase (PARP) and Tankyrase. According to the present invention, a colorectal treatment effect can be maximized by sorting patients having sensitivity to the simultaneous inhibitor against PARP and Tankyrase.

Abstract: The present invention relates to a method of diagnosing pancreatic cancer through bacterial metagenomic analysis, and more particularly to a method of diagnosing pancreatic cancer by analyzing an increase or decrease in content of specific bacteria-derived extracellular vesicles through metagenomic analysis using a subject-derived sample. Extracellular vesicles secreted from bacteria existing in the environment are absorbed into the human body, and thus may directly affect the occurrence of cancer, and it is difficult to diagnose pancreatic cancer early before the onset of symptoms so that efficient treatment thereof is difficult.

Abstract: The present invention provides a method for treating human cancer, which comprises predicting the sensitivity of tumor cells or tissues in a cancer patient to DNA damage therapy; and administering DNA damage therapy to the cancer patient. The invention also relates to kits to perform the method.

Abstract: Methods and systems for assessing the risk of a human female subject for developing breast cancer. In particular, the present disclosure relates to combining a first clinical risk assessment, a second clinical assessment based at least on breast density, and a genetic risk assessment, to obtain an improved risk analysis.

Abstract: Presented herein are methods and compositions for determining MSI status and LS screening in a single test of colorectal cancer tissue. Also presented herein are methods for determining MSI status in a sample of cell-free DNA obtained from blood obtained from a cancer patient.

Abstract: A Sidwf1 gene of Sesamum indicum, including two exons and an intron, is 1638 bp in total, and has a sequence represented by SEQ ID NO: 1. Also provided is a method for determining the internode length type in sesame samples, the method including: 1) extracting a genomic DNA of a sesame sample; 2) synthesizing three primers including SiSNPdwf1 F1, SiSNPdwf1 F2, and SiSNPdwf1 R; amplifying the Sidwf1 gene or an allele SiDWF1 thereof with the genomic DNA of the sesame sample as a template, with a combination of SiSNPdwf1 F1, SiSNPdwf1 F2, and SiSNPdwf1 R a combination of SiSNPdwf1 F1 and SiSNPdwf1 R, or a combination of SiSNPdwf1 F2 and SiSNPdwf1 R, as primers, thereby yielding a PCR product; and performing electrophoresis on the PCR product or sequencing the PCR product, and determining the phenotype of the sesame sample according to an electrophoresis or sequencing result.

Abstract: Sensitive and specific detection of nucleic acids can be achieved using a chemical ligation-based template assisted rapid assay (TARA-L) with simple chemical reactions between probes and without the need for enzymes. Probes are designed to form a ligation product when they anneal to adjacent portions of a target nucleic acid. The ligation products can be detected, such as in immunochromatographic assays. The methods allow for the fast, efficient analysis of biological samples for the presence of nucleic acids and can be used, for example, in point of care settings.

Abstract: A stretching apparatus for stretching a pelt on a pelt board, wherein the pelt has a substantially tubular shape defining an inwardly oriented leather side, an outwardly oriented fur side, a nose end, a rear end and a pair of front leg cavities. The pelt board defines a top end for accommodating and fixating the nose end of the pelt, a circumferential wall for facing the inwardly oriented leather side of the pelt and a base end located opposite the top end. The stretching apparatus comprises a holding device for holding the base end of the pelt board, and a stretching device having a pair of stretching members. Each of the stretching members are adapted in order to be inserted into a respective front leg cavity of the pelt and the stretching device is movable in a direction towards the holding device for stretching the pelt.

Abstract: The present invention preferably comprises a system and method for operating and/or positioning a lance and nozzle assembly relative to a molten metal bath in a vessel. Specifically, at least one temperature sensor is provided proximate a tip of the lance and nozzle assembly and at least one temperature sensor is provided on or in the vessel. A processing unit is configured to receive at least one signal from each of the temperature sensors, process the signals to determine the active position of the lance and nozzle assembly relative to the metal bath, and move the lance and nozzle assembly to a preferred position the corresponds to a stage of operation in the vessel.

Abstract: A delivery device that is usable in a laser peening operation emits a columnar flow of a fluid that contains therein a beam of electromagnetic energy. The beam is retained within the interior of the flow of fluid since the total internal reflectivity of the flow is sufficient to do so. The flow of fluid that serves as a conduit for the beam also itself impinges on a workpiece and thus contains and washes away the plasma that forms from a laser peening operation, and this resists the plasma from reaching and possibly damaging the delivery device. The carrying of the beam in the columnar flow of fluid avoids the need to maintain a fixed distance between the delivery device and the workpiece, which simplifies the movement by a robotic manipulator of the delivery device along a non-planar surface of a workpiece during a laser peening operation.

Abstract: Methods and masks for manufacturing component of gas turbine engines are described. The methods include applying a mask to a protected surface of the component, the component having a designated surface to be treated by a shot peen operation. The mask includes a full masking portion configured to prevent a shot peen media from impacting the protected surface. A masking control region is arranged around the designated surface. The masking control region is configured to control an amount of force imparted to the component by shot peen media during the shot peen operation, wherein the masking control region extends from the full masking portion to the designated surface. The designated surface is shot peened with shot peen media to form a compressive stress region within the component proximate the designated surface and a tapering transition of compressive forces within the component proximate the masking control region.

Abstract: Disclosed is an austenitic light weight steel which may have decreased specific gravity while maintaining excellent strength and ductility. The austenitic light weight steel may include carbon (C) in an amount of about 0.8 to 1.3 weight %, aluminum (Al) in an amount of about 9.0 to 15.0 weight %, manganese (Mn) in an amount of about 10.0 to 20.0 weight %, nickel (Ni) in an amount of about 0.25 weight % or less (excluding 0 weight %), chromium (Cr) in an amount of about 0.5 weight % or less (excluding 0 weight %), silicon (Si) in an amount of about 0.5 weight % or less (excluding 0 weight %), a sum of titanium (Ti) and vanadium (V) in an amount of about 1.0 weight % or less (excluding 0 weight %), other unavoidable impurities, and iron (Fe) constituting the remaining balance, based on the total weight of the light weight steel.

Abstract: Disclosed is a hot-pressed member that can exhibit very high tensile strength after hot pressing as high as TS: 1780 MPa or more, excellent resistance to resistance welding cracking, and excellent delayed fracture resistance after resistance welding by having a specific chemical composition, and a microstructure such that a prior austenite average grain size is 7 ?m or less, a volume fraction of martensite is 90% or more, and at least 5 Nb-based precipitates having a grain size of less than 0.08 ?m are present on average per 100 ?m2 of a cross section parallel to a thickness direction of the member within a range of 100 ?m in the thickness direction from a surface of the member, and such that a Ni diffusion region having a thickness of 0.5 ?m or more is present in a surface layer of the member.

Abstract: A method for the manufacture of a galvannealed steel sheet including the provision of a specific steel sheet, a recrystallization annealing with specific heating, soaking and cooling sub-steps using an inert gas, a hot-dip galvanizing and an alloying treatment; the galvannealed steel sheet and the use of the galvannealed steel sheet.

Abstract: Abrasion resistant steel having a chemical composition comprising, by mass %, C: 0.10 to 0.20%, Si: 0.01 to 1.20%, Mn: 0.01 to 2.00%, P: less than 0.017%, S: 0.010% or less, Cu: 0.01 to 0.70%, Ni: 0.01 to 1.00%, Cr: 0 to 1.50%, Mo: 0 to 0.80%, W: 0 to 0.50%, Nb: 0 to 0.050%, V: 0 to 0.20%, Ti: 0 to 0.030%, B: 0 to 0.0030%, N: 0.0001 to 0.0070%, Al: 0.001 to 0.10%, Ca: 0 to 0.0050%, Zr: 0 to 0.0050%, Mg: 0 to 0.0050%, REM: 0 to 0.0050%, and a balance of Fe and impurities, having metal structures at a position of ¼ of the thickness from the surface in a thickness direction, which have a total of the area ratios of martensite and lower bainite of 50 to 100% and a prior austenite average grain size of 5 to 23 ?m, and having a Brinell hardness at a position of 1 mm from the surface in the thickness direction of 360 to 440, and a method for producing the same.

Abstract: A grain oriented electrical steel sheet includes: a base steel sheet; a glass film which is arranged in contact with the base steel sheet; and an insulation coating which is arranged in contact with the glass film and which includes a phosphate and a colloidal silica as main components. The base steel sheet includes the predetermined chemical composition. A BN whose average particle size is 50 to 300 nm is included at a predetermined number density in a region which is from an interface between the glass film and the insulation coating till 5 ?m toward the base steel sheet in a depth direction. A B emission intensity obtained inside the glass film is more than a B emission intensity obtained inside the base steel sheet, when a B emission intensity is measured from a surface of the insulation coating by a glow discharge emission spectroscopy.

Abstract: A high-strength steel wire includes a predetermined chemical composition. In the high-strength steel wire, a metallographic microstructure includes a pearlite structure of not less than 95% by area, an average aspect ratio R of a pearlite block, measured in a surface layer in a cross section in an axial direction including an axis of the steel wire, is 2.0 or more, assuming that a diameter of the steel wire is D, a ratio of an average aspect ratio measured in the surface layer to an average aspect ratio measured at a position of 0.25D is 1.1 or more in the cross section in the axial direction including the axis of the steel wire, and a tensile strength of the steel wire is 1,800 MPa or more.

Abstract: The invention relates to a processing method of a battery pack that includes at least a battery body and a resin. The processing method includes a step of covering the battery pack with a slag that is at a predetermined temperature.

Abstract: A method for treating converter slag for the purpose of recirculating iron, wherein a converter slag is brought into contact with oxygen in such a way that by means of turbulence, the slag is mixed, the iron and iron oxide components that are present are oxidized, and the slag is then allowed to stand in the vessel or a vessel until a segregation into a solidifying, silicate and phosphorus-rich first fraction and an underlying liquid iron oxide-rich second fraction has taken place, with the converter slag that is used being mixed with a partial flow from the iron oxide-rich second fraction in such a way that the total FeO content of the slag that is to be treated with oxygen is over 35% by weight, thus enabling the segregation into two fractions.

Abstract: The invention relates to a beneficiation process for uranium ores comprising clay and carbonate minerals, the process comprising: performing a hydrocyclone step to obtain a hydrocyclone underflow fraction substantially comprising the uranium component; treating the hydrocyclone underflow fraction to effect a separation of carbonate and uranium minerals; and recovering the uranium-bearing minerals to produce a uranium concentrate.

Abstract: The present invention relates to a copper alloy for dental prosthesis and specifically, to a copper alloy for dental prosthesis having such color and luster that the copper alloy can be used as a substitute for a gold alloy and having excellent resistance to corrosion, wherein the copper alloy comprises, in wt %, 7-10% of Al, 1-2.5% of In, 1-2.5% of Sn, 2-4% of Fe, 2-3.5% of Ni, 2.5-4% of Mn, 2-5% of Zn, and the balance Cu.

Abstract: An alpha-beta titanium-based alloy including titanium; one of 0.001-1.0 wt. % neodymium, 0.001-1.0 wt. % dysprosium, or 0.001-0.5 wt. % erbium; and at least one of aluminum, zirconium, tin, oxygen, molybdenum, vanadium, niobium, iron, and chromium present in amounts defined based on an aluminum equivalent and a molybdenum equivalent, wherein the aluminum equivalent (Al-eq) is between 0 to 7.5% and the molybdenum equivalent (Mo-eq) is between 2.7 to 47.5, and wherein the aluminum equivalent (Al-eq) and the molybdenum equivalent (Mo-eq) are defined, in weight percents, as follows: Al-eq=(Al %)+(Zr %)/6+(Sn %)/3+10*(O %) Mo-eq=(Mo %)+0.67*(V %)+0.33*(Nb %)+2.9*(Fe %)+1.6*(Cr %).

Abstract: A nickel -titanium alloy is made to be wholly or substantially free of titanium-rich oxide inclusions by including yttrium in an amount up to 0.15 wt. %, with the balance of the alloy being nickel and titanium in approximately equal proportion. For example, a NiTiY alloy may have a composition including, in weight percent based on total alloy weight: between 50 and 60 wt. % nickel; between 40 and 50 wt. % titanium; and between 0.01 and 0.15 wt. % yttrium. The resulting alloy is capable of being drawn into various forms, e.g., fine medical-grade wire, without exhibiting an unacceptable tendency to develop surface defects or to fracture or crack during cold drawing or forging. The resulting final forms exhibit favorable fatigue strength and fatigue-resistant characteristics.

Abstract: An aluminum alloy, comprising: 4.0 to 10.0 weight % silicon (Si), 0.1 to 4.0 weight % magnesium (Mg), 0.1 to 1.0 weight % chromium (Cr), 0.05 to 1.0 weight % zinc (Zn), 0.05 to 1.0 weight % manganese (Mn), 0.01 to 1.0 weight % titanium (Ti), 0.001 to 0.5 weight % tin (Sn), and 81.5 to 95.689 weight % aluminum and at least one impurity.

Abstract: A magnesium composite material and method for manufacturing a magnesium composite alloy wherein the magnesium composite alloy has improved thermal and mechanical properties. The improved thermal and mechanical properties are at least in part obtained by the modification of grain boundary properties in the magnesium composite alloy by the addition of nanoscale fillers to the magnesium composite alloy.

Abstract: A magnesium alloy containing Al, Sr, Ca, and Mn, with the balance being Mg and inevitable impurities, the magnesium alloy having: a structure having an ?-Mg phase, and a precipitate dispersed in at least one of a grain boundary of the ?-Mg phase and a cell boundary, the precipitate including: at least one phase selected from a group A consisting of an Al2Sr phase, an Al4Sr phase, a (Mg, Al)2Sr phase, and a (Mg, Al)4Sr phase; and at least one phase selected from a group B consisting of an Al2Ca phase and a (Mg, Al)2Ca phase, the magnesium alloy having, in a cross section, a total area rate of a group A precipitate and a group B precipitate of greater than or equal to 2.5% and less than or equal to 30%.

Abstract: A Fe—Ni based alloy comprising, in weight percent: Ni 30-35; Cr 12-14; Al 3-5; Ti 0-2; Ta 2-8; C<=0.05; B<=0.005; Zr<=0.2; Si<0.5; where Cr/(Cr+Fe+Ni)=0.125-0.145; Al/(Al+Ti+Ta)=0.15-0.5; and Fe?Ni; balance Fe, the alloy having a face-centered cubic (fcc) matrix with from 25 to 30 vol. % of L12-type ??-Ni3M (M=Al, Ta, Ti and mixtures thereof) precipitates.

Abstract: Provided are: an oriented electrical steel sheet having a high tension applied to a steel sheet and excellent adhesion to a film; and a method for producing the same. This oriented electrical steel sheet includes: a steel sheet; a film A containing a crystalline material disposed on the steel sheet; and a film B containing a vitreous material disposed on the film A, wherein an element profile, which is obtained by using a high-frequency glow discharge light-emission surface analysis method, in the direction from the film B to the steel sheet satisfies formulae (1) and (2). 0.35?(tA/tFe/2)?0.75 . . . (1), 0.25?(tA/2/tFe/2)?1.00 . . . (2), where tA represents the peak time of an alkali metal element profile, tA/2 represents the half time of an alkali metal.

Abstract: The present invention relates to high-strength medium manganese steel for warm stamping, which contains 3-10 wt % of manganese (Mn), 0.05-0.3 wt % of carbon (C), and 0.1-1.0 wt % of silicon (Si) as components thereof, with the balance being iron (Fe) and unavoidably contained impurities. The present invention performs heat treatment at the low austenitizing temperature of medium manganese steel, and thus has the effect of reducing the high thermal energy consumption of the prior art hot stamping process. Furthermore, the present invention does not require an additional temperature process, and can obtain high strength by only slow cooling such as air cooling outside a mold without performing cooling at high rate inside the mold, and thus has the effects of simplifying a process and improving manufacturing efficiency.

Abstract: A cold rolled and heat treated steel sheet having a composition with the following elements, expressed in percentage by weight 0.10%?Carbon?0.5%, 1%?Manganese?3.4%, 0.5%?Silicon?2.5%, 0.03%?Aluminum?1.5%, Sulfur?0.003%, 0.002%?Phosphorus?0.02%, Nitrogen?0.01% and can contain one or more of the following optional elements 0.05%?Chromium?1%, 0.001%?Molybdenum?0.5%, 0.001%?Niobium?0.1%, 0.001%?Titanium?0.1%, 0.01%?Copper?2%, 0.01%?Nickel?3%, 0.0001%?Calcium?0.005%, Vanadium?0.1%, Boron?0.003%, Ceriums?0.1%, Magnesiums?0.010%, Zirconiums?0.010% the remainder composition being composed of iron and the unavoidable impurities caused by processing, and a microstructure of the said rolled steel sheet having by area fraction, 10 to 30% Residual Austenite, 5 to 50% Annealed Bainite, 10 to 40% of Bainite, 1% to 20% Quenched Martensite, and less than 30% Tempered Martensite where the combined presence of Bainite and Residual Austenite shall be 30% or more.

Abstract: A process of heat treating an Al—Si—Cu—Mg—Fe—Zn—Mn—Sr-TMs alloy, where the TMs include Zr and V, includes heat treating the alloy to produce a microstructure having a matrix with Zr and V in solid solution after solidification. The solid solution Zr, in wt. %, is at least 0.16%, the solid solution V, in wt. %, is at least 0.20% after heat treatment, and Cu and Mg are dissolved into the matrix during the heat treatment and subsequently precipitated during the heat treatment. The composition of the alloy, in wt. %, includes Cu between 3.0-3.5%, Fe between 0-0.2%, Mg between 0.24-0.35%, Mn between 0-0.40%, Si between 6.5-8.0%, Sr between 0-0.025%, Ti between 0.05-0.2%, V between 0.20-0.35%, Zr between 0.2-0.4%, maximum 0.5% total of other alloying elements, and balance Al.

Abstract: A black member includes a base and a black layer laminated on the base. The black layer contains titanium aluminum nitride, titanium silicon nitride, or titanium aluminum silicon nitride. The black layer may also contain at least one type of elements selected from the group consisting of oxygen, fluorine, and carbon. When the black layer contains carbon, and assuming that a total amount of elements contained in the black layer is 100 at %, the black layer contains carbon of 10 at % or less. In a color evaluation according to an L*, a*, b* color system (CIE color system), the black layer satisfies L*?48.0, ?2.0?a*?3.0, and ?3.5?b*?3.0.