Abstract: A fully automated chemilumiescence immunoassay analyzer, including a sample and reagent receiving device for receiving a sample and a reagent, a dispensing device for aspirating and discharging the sample and the reagent, a mixing device for mixing the sample and the reagent in a reaction vessel, an incubation and luminescence detection device for incubation and luminescence detection, a magnetic separation cleaning device for separation cleaning an analyte and impurities in the reaction vessel, a reaction vessel grasping device for transferring the reaction vessel, and a liquid path device. The fully automated chemiluminescence immunoassay analyzer has a simple structure and is convenient to operate, and also reduce the overall size such that the footprint thereof is small and the production cost is reduced, so that the analyzer is easy to achieve miniaturization, and is convenient for an operator to use.

Abstract: Acoustically responsive particles and methods are provided for their use. Methods are provided for making and using tunable, monodisperse acoustically responsive particles and negative contrast acoustic particles, wherein the particles can contain a functional group available for covalent modification.

Abstract: A micro-resonator and fiber taper based sensing system, which uses mode splitting or frequency shift methods and polarization measurements for particle sensing.

Abstract: To provide an immunochromatographic test device capable of accurate diagnosis even when an excess sample is introduced. Provided is the immunochromatographic test device consisting: a test strip; a lower housing including a plurality of support bases that support the test strip; and an upper housing including a dropping hole for dropping a sample into the test strip and a detection window in a direction in which the sample introduced from the dropping hole develops on the test strip, wherein a width of the support base that supports portion of the test strip exposed from the detection window is smaller than a width of the test strip, or wherein among the plurality of support bases, the width of the support base arranged on the lower housing between a position corresponding to the detection window and a position corresponding to the dropping hole is larger than the width of the test strip.

Abstract: The present invention provides a method for identifying an exosome surface molecule, including blocking and washing a carrier having a binding molecule to the exosome surface molecule immobilized thereon with a casein solution or a decomposed casein solution, and mixing a casein solution or a decomposed casein solution and a test sample containing an exosome before contact of the carrier and the test sample.

Abstract: The present disclosure relates to systems and methods for assessing viral, e.g., HIV, infection duration in a subject. More specifically, the present disclosure relates to for assessing viral, e.g., HIV, infection duration in a subject using, inter alia, a reader configured to measure both the number of signal pixels and the intensities of signal pixels to generate a quantitative signal readout that is used to assess average antibody avidity of an anti-viral antibody, e.g., an anti-HIV antibody, in a sample liquid and/or viral, e.g., HIV, infection duration in a subject.

Abstract: Provided are novel methods for screening and testing for pathogens in food, water, and bodily fluids using methods that are faster to complete than conventional methods of culturing and plating that require lengthy times in properly equipped labs. The invention utilizes specific, rapid and sensitive optical detection to capture small concentrations of the target bacteria and render them amenable for detection with various specific synthesis binding agents approaches. The technique merges capture and detection steps with quantification unit suitable to provide results in a relatively shorter time current detection methods.

Abstract: Disclosed is a system and method for a microdevice to separate blood cells based on differences in antigen expression. Specifically, cells of the same phenotype are separated based on whether or not they are activated during infection or resting. The device of the present disclosure takes a small sample of blood and provides differential cell counts that can be used to test for infection and inflammatory response. The device can be used to identify sepsis and other infections rapidly. By measuring differences in activated white cell counts such as neutrophils, the device of the present disclosure measures physiological response to infection in hospitalized patients recovering from burns, surgeries, etc.

Abstract: Neutrophils contribute to demyelinating autoimmune diseases, yet their phenotype and functions have been elusive to date. The present application shows that ICAM1 surface expression distinguishes extra- from intravascular neutrophils. Transcriptomic analysis of these two subpopulations indicated that neutrophils, once extravasated, acquire macrophage-like properties, including the potential for immunostimulation and MHC class II-mediated antigen presentation. Further, the present application shows that aspartic retroviral-like protease ASPRV1 is specifically expressed by neutrophils in the mouse and human immune system, and correlates with CNS neutrophil infiltration in EAE, multiple sclerosis and neuromyelitis optica.

Abstract: The present invention discloses a reagent set for detecting interactions between biomolecules and their regulatory factors and applications. One reagent set disclosed in the present invention consists of three reagents named A, B and C; the reagent A is formed by connecting a biomolecule R and a biomolecule X; the reagent B contains a biomolecule L; there is an interaction between the biomolecule R and the biomolecule L, and a phase transition occurs when the biomolecule R and the biomolecule L interact; the reagent C is formed by connecting a reporter group JIA with a biomolecule named XL.

Abstract: Aspects of the present invention provide a monoclonal antibody specific for BQ323636.1 and its use for prediction of tamoxifen resistance in breast cancer patients.

Abstract: The present invention relates to a type XVI collagen assay and its use in evaluating diseases associated with type XVI collagen, in particular colorectal cancer and ulcerative colitis, and for identifying a subgroup of patients with Crohn's disease that have (or are likely to develop) fibrostenotic strictures.

Abstract: Immune context scores are calculated for stage III colorectal tumor tissue samples using continuous scoring functions. Feature metrics for at least one immune cell marker are calculated for a region or regions of interest, the feature metrics including at least a density of human CD3+ cells in a region of interest including an invasive margin. A continuous scoring function is then applied to a feature vector, the output of which is an immune context score. The immune context score may then be plotted as a function of a diagnostic or treatment metric, such as a prognostic metric (e.g. overall survival, disease-specific survival, progression-free survival) or a predictive metric (e.g. likelihood of response to a particular treatment course). The immune context score may then be incorporated into diagnostic and/or treatment decisions.

Abstract: A method of diagnosing lung cancer in a subject-in-need thereof is provided. The method comprises: (a) providing a biological sample of the subject which comprises peripheral blood mononuclear cells (PBMCs); (b) in vitro contacting the PBMCs with a stimulant selected from the group consisting of the stimulants listed in Tables 3 and 4; and (c) measuring metabolic activity of the PBMCs having been contacted according to (b), wherein a statistically significant change in the metabolic activity of the PBMCs as compared to a control sample is indicative of lung cancer.

Abstract: The disclosure provides methods for analyzing rare circulating cells (RCCs) at cellular and molecular level following their detection in non-enriched blood samples, methods of this disclosure serve as diagnostic methods for several disease conditions, including cardiovascular diseases and cancer.

Abstract: Provided herein are gold nanoparticle based colorimetric methods for the detection of spermine in a sample. The methods can be used to diagnose prostate cancer in a subject based on the detected amount of spermine in a urine sample obtained from the subject.

Abstract: The present disclosure provides a method for detecting cholangiocarcinoma cells. The capture rate of the cholangiocarcinoma cells of the present disclosure is higher than 70%, and a plurality of octasaccharides with high affinity and specificity can be modified on the surface of magnetic beads to capture and analyze cholangiocarcinoma cells under test, wherein the cholangiocarcinoma cells can be circulating tumor cells in cholangiocarcinoma.

Abstract: PI3K signalling is the most increased pathway in human cancers. The four isoforms of PI3K are thought to be activated by different redundant mechanisms leading to a common downstream signalling. However, the mutational pattern of PI3K pathway or its level of expression is not sufficient to predict the sensitivity to PI3K inhibitors. By identifying for the first time a phosphopeptide that predict the sensitivity to p110? and/or p110? inhibitors, the inventors provide insight in how to handle heterogeneity of PI3K expression patterns in tumoral samples for the choice of available PI3K-targetting drugs. Accordingly, the present relates to a phosphopeptide characterized by the amino acid sequence as set forth in SEQ ID NO:1 (PGTPSDHQSQEASQFER) wherein the threonine residue at position 3 is phosphorylated.

Abstract: The present invention relates to a method for evaluating a drug involved in angiogenesis. The method includes a drug administration step (A) of administering a drug involved in angiogenesis to a subject having a lesion. The method further includes: an imaging step (1) of performing radiography on a subject in a state where metal nanoparticles are present in a blood vessel or an imaging step (2) of performing fluorescence staining and fluorescence imaging using a dimeric protein; and evaluating the drug using an image acquired in the imaging step (1) or (2).

Abstract: This disclosure relates to the field of mass spectrometry analysis. In some embodiments, the disclosure relates to compositions and methods for detecting and quantifying proteins in the RAS-RAF-MAPK pathway by immunoprecipitation enrichment followed by mass spectrometry analysis.

Abstract: A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be C60 fullerene. Emission correlates with cellular membrane potential.

Abstract: Provided is a method for identifying proteins capable of increasing the number of identified proteins contained in a target sample derived from blood. A protein contained in the target sample derived from blood is fragmented, and a protein contained in an having less bias in a quantity ratio of proteins than the target sample is fragmented, and the fragmented proteins are mixed (Steps S101 to S103). In this manner, the mixed sample in which the bias in a quantity ratio of proteins is less than that of the target sample is generated. By performing MS/MS measurement using the generated mixed sample (Step S107), an MS/MS spectrum of a peak derived from a protein contained in a small amount in the target sample can be prevented from being missed. Accordingly, the number of identified proteins contained in the target sample derived from blood can be increased.

Abstract: Disclosed herein are methods and compositions for using nanoexpression to interrogate antigen specificity within antibody repertoires. Also disclosed herein is an antibody display system composition comprising one or more recombinant nucleic acid sequences comprising a first nucleic acid sequence encoding a heavy chain variable region sequence or fragment thereof operatively linked to a first identification region sequence and a second nucleic acid sequence encoding a light chain variable region sequence or fragment thereof operatively linked to a second identification region sequence.

Abstract: The therapeutic effect of and/or prognosis after administration of an anti-VEGFR-2 antibody drug, in particular, ramucirumab can be predicted by measuring VEGF-A, VEGF-D, sVEGFR-2, SDF-1?, and/or cNRP1. Provided is a biomarker for predicting the effect of administration of the anti-VEGFR-2 antibody drug, in particular, ramucirumab, and a test method and a test kit using the marker.

Abstract: The present invention is related to a method for differential diagnosis of hereditary angioedema, wherein the method comprises determining the level of C4 protein, C1-INH protein and C1q protein in a sample from a subject, wherein the sample is a dried blood spot sample and wherein the level is determined by mass spectrometry.

Abstract: Methods, compositions and kits useful in the detection, assessment, diagnosis, prognosis and/or treatment of neurological injury or disease or brain injury, such as traumatic brain injury (TBI), are provided in which certain newly discovered protein biomarkers are detected in a biological sample of a subject undergoing testing or evaluation. The methods allow for detection of changes in levels, amounts, or concentrations of the protein biomarkers in a subject compared with those of controls. Detection of the protein biomarkers, and/or levels thereof, provides an indication of biological and biochemical events, e.g., at a cellular level, that are occurring in the subject who is undergoing testing or analysis for the neurological injury or brain injury.

Abstract: A hemoglobin detecting method is executed by a mobile device having a hemoglobin detecting function. The mobile device includes a processor unit, a first light source that generates a first light beam, and a light detecting module that receives a second light beam that is generated when the first light beam travels through an analyte solution and is reflected. The light detecting module generates first to fourth intensity signals according to the second light beam, and the processor unit determines whether the absorption spectrum of the analyte solution matches a target spectrum. If the absorption spectrum of the analyte solution matches the target spectrum, the processor unit generates positive result information; otherwise, the processor unit generates negative result information. The mobile device provides a fast and accurate way to detect blood in a stool solution, which does not require collecting samples of stool or applying any chemical.

Abstract: A detection of insulin in saliva and methods of use thereof, may include methods for detecting or predicting a postprandial blood or plasma insulin response to a foodstuff, said method comprising: obtaining a first saliva sample from the subject; obtaining a second saliva sample from the subject following ingestion of a foodstuff; measuring a first level of insulin in the first saliva sample; measuring a second level of insulin in the second saliva sample; and characterizing the subject's blood or plasma insulin level based on a difference between the first level of insulin and the second level of insulin, to thereby detect or predict a postprandial blood or plasma insulin response to the foodstuff; the detection of insulin in saliva and methods of use thereof may also include methods for determining metabolic status, and methods for diagnosing and/or monitoring hyperinsulinemia in a subject.

Abstract: The present invention provides in vitro test kits, assays and methods useful for screening a test sample for the presence of a ligand which is characterized by its ability to form a complex with a steroid hormone receptor and elicit a genomic response when in a cell. Advantageously, the activity-based assays which form the basis of the test kits and methods described herein, are particularly useful in detecting the presence of a ligand of unknown structure, for example, a designer drug used by equine, canine and human athletes in sports doping. Different assay prototypes are disclosed in which activation of the hormone receptor by a ligand binding interaction may be detected, for example, though activation of a reporter molecule. In certain examples, the present invention provides test kits, assays and methods involving aptame: fluorophore reporter constructs for detection of a ligand from (e.g.) a sample taken trackside from an athlete.

Abstract: In a method for immunologically quantifying a steroid, it is possible to exclude a steroid from cyclodextrin when a standard solution containing the steroid included in cyclodextrin is treated with a surfactant, which thereby suppresses the deviation in behavior of the antigen-antibody reaction between the specimen (test sample) and the standard solution, and then significantly improves the accuracy of steroid quantification.

Abstract: The present invention provides methods for identification of subjects with a rise in plasma coagulability post-diagnosis of thrombotic event (and consequent increased risk for recurrent thromboembolism) as well as for identification of subjects without thrombotic event who exhibit a rise in plasma coagulability for which prophylactic anticoagulation may be warranted for TE prevention, and other methods for identification of perioperative subjects who are at heightened risk for perioperative bleeding and/or for requiring blood transfusion, using the CloFAL assay, to aid in clinical decision making regarding anticoagulant and/or adjunctive clinical management designed to mitigate this heightened thromboembolic risk.

Abstract: The present invention discloses a new approach to produce membrane or lipid bilayer mimicking surfaces, their use in the aforementioned areas of application, a kit of parts and a sensor.

Abstract: The subject invention provides methods, assays, and products for visual detection of small-molecule targets in a sample in both clinical and field settings within minutes. The subject invention is based on an aptamer sensor that reports the presence of small-molecule target via a sensitive colorimetric signal for naked-eye detection. The aptamer sensor is a CBSAzyme-based sensor having both target-mediated cooperative behavior of the CBSA and peroxidase-mimicking catalytic activity of DNAzyme. The subject invention also provides methods of using the CBSAzyme-based sensor.

Abstract: Systems and methods for dynamic automation are provided. An automation module, for example, may receive at least one map template that is selectable by a user. Based on the map template, the automation module may dynamically generate a robot deck layout map and/or procedure and also generate a series of robotic commands for a robot. The robotic commands may then be passed to the robot to carry out the automation. Accordingly, the automation may be entirely dynamic, without the need for a user to pre-program or pre-assemble all of the robotic commands prior to the automation. The automation module may thus be able to build, in real-time and/or on the fly, the robotic commands based only on the at least one map template that was selected by the user.

Abstract: In the field of pathological anatomy, in particular the sector related to the reading of slides used in hospital departments, disclosed is an automated system for reading codes printed on the slides.

Abstract: A sample identification system for an automated sampling device is described. A system embodiment includes, but is not limited to, a sample holder having a plurality of apertures configured to receive a plurality of sample vessels therein, the sample holder having one or more corresponding sample holder identifiers positioned proximate to the sample holder; and an identifier capture device configured to detect the one or more sample holder identifiers positioned proximate to the sample holder and generate a data signal in response thereto, the data signal corresponding to at least an orientation of the sample holder relative to a surface on which the sample holder is positioned.

Abstract: A sample measuring apparatus and a method of circulating air in a reagent storage are provided. The sample measuring apparatus includes: a measuring unit that measures a sample by using a reagent stored in a reagent container; a reagent storage that stores the reagent container; a reagent container holder arranged in the reagent storage and that holds the reagent container; a fin that circulates air in the reagent storage; and a driving unit that drives the reagent container holder and the fin to rotate.

Abstract: A sample measuring apparatus, a reagent container, and a method of measuring a sample are provided. The sample measuring apparatus includes a reagent container holder and a measuring unit. The reagent container holder holds, by suspending, a reagent container that stores a reagent. The measuring unit that measures a sample by using the reagent.

Abstract: A sample measuring apparatus, A reagent container, and a method of measuring a sample are provided. The sample measuring apparatus includes: a container holding unit that holds a reagent container comprising an openable/closable lid portion that covers an opening of the reagent container; a reagent dispensing unit that aspirates a reagent in the reagent container with the lid portion being in an opened state and dispenses the aspirated reagent into a reaction container; a pressing unit that seals the opening, by pressing toward the opening the lid portion laid over the opening; and a measuring unit that measures components contained in a measurement specimen prepared from a sample and the reagent dispensed in the reaction container.

Abstract: A hand-portable test apparatus includes an in-the-field test processing assembly, and a lab-on-a-chip test cartridge having a neutralising zone, a specific reagent mixing zone, and a testing chamber. It has a convective heating loop for thermal cycling. There are two passive self-actuating valves that allow the test chamber volume to fill with solution, but then close to meter and trap the solution. The apparatus has external illumination ports, and an optical sensing port. Each cartridge is uniquely identified. It has smooth surfaces that allow adhesive membranes to be used to permit the pre-loading of reagents, prevent evaporation, and permit preservation of results. The test apparatus includes a holder for the cartridge with a heater, illumination, and optical sensor units closely positioned relative to the holder. There is a wiring circuit board, a processor, and a power supply. All of the items are contained within a unitary housing.

Abstract: A method for determining a volume of fluid dispensed into a test housing includes creating an electrical potential across at least one input electrode of a plurality of input electrodes and at least one output electrode of a plurality of output electrodes. The input electrodes and the output electrodes are each coupled to the test housing. The method includes receiving at least one signal from the at least one output electrode based on the fluid dispensed into the test housing. The method includes calculating the volume of fluid dispensed into the test housing based on the at least one signal received from the at least one output electrode, a dimension associated with an internal channel defined within the test housing, and a distance between two input electrodes of the plurality of input electrodes.

Abstract: The cell transfer device includes a head group including a plurality of heads to which tips are attached and which move along a first direction; a head unit in which the head group is installed and which moves in a second direction and in a third direction; and a plurality of drive motors which are mounted on the head unit and which generate driving force to cause the head to move along the first direction. The plurality of drive motors are separately arranged on one side and the other side in the third direction with the head group provided therebetween. The head group includes a first head and a second head. The first head is driven by the drive motor arranged on the one side in the third direction, and the second head is driven by the drive motor arranged on the other side in the third direction.

Abstract: Systems and methods are described to determine whether a sample transmitted through a transfer line from a remote sampling system contains a suitable sample to analyze by an analysis system. A system embodiment includes, but is not limited to, a sample receiving line configured to receive a liquid segment a first detector configured to detect the liquid segment at a first location in the sample receiving line; a second detector configured to detect the liquid segment at a second location in the sample receiving line downstream from the first location; and a controller configured to register a continuous liquid segment in the sample receiving line when the first detector and the second detector match detection states prior to the controller registering a change of state of the first detector.

Abstract: A magnetic field sensor for detecting motion of an object includes one or more magnetic field sensing elements configured to generate a magnetic field signal in response to a magnetic field associated with the object. A motion detector responsive to the magnetic field signal and to a threshold signal is configured to generate a detector output signal having edges occurring in response to a comparison of the magnetic field signal and the threshold signal. A speed detector responsive to the detector output signal generates a speed signal indicative of a speed of motion of the object. A delay processor is responsive to the speed signal and configured to determine a delay for the detector output signal based on the speed of motion of the object.

Abstract: A magnetic roller device and a method for calculating rotation information thereof are provided. The magnetic roller device includes a multipole magnet (1), an MCU, plurality of Hall components (2), and a roller disposed on a handwriting device. The multipole magnet (1) is disposed on the roller, the multipole magnet (1) includes at least one pair of magnetic poles with opposite polarities, and output ends of the plurality of Hall components (2) are connected to an input end of the MCU. The magnetic roller device composed of the multipole magnet (1) and the roller performs data adjustment of the handwriting device in place of a mechanical roller and a touch roller, and rotation information of the magnetic roller device is calculated and acquired in conjunction with the plurality of Hall components (2) and the MCU.

Abstract: Provided is a method of detecting a gait parameter through a head part acceleration sensor. The method of detecting a gait parameter through a head part acceleration sensor includes: a gait acceleration collecting step; a gait point-in-time detecting step; and a gait parameter detecting step, wherein the start point and the end point of the double-limb support are set within a window predetermined on the basis of peaks of the gait cycle, the start point of the double-limb support is the same as an end point of a single-limb support, and the end point of the double-limb support is the same as a start point of a single-limb support.

Abstract: A MEMS accelerometer, including: a substrate, a movable component and a fixed electrode group, wherein a surface of the substrate has an anchoring region; the movable component is connected to the anchoring region through a supporting beam and suspended above the substrate, and the movable component includes a first proof mass and a second proof mass; the first proof mass has a first hollowed-out region in the middle, the first hollowed-out region is I-shaped, and the second proof mass is located in the first hollowed-out region; and the fixed electrode group includes a first electrode group, which is fixed on the surface of the substrate, located between the substrate and the movable component, and forms a Z-axis detection capacitor bank with the first proof mass and the second proof mass.

Abstract: An accelerometer comprising a plurality of proof-masses moveable along a measurement axis; a respective spring rigidly attached to each proof-mass, configured to exert an elastic recall on the proof-mass in the measurement axis; and a fixed stop associated with each proof-mass, arranged to intercept the proof-mass when the acceleration in the measurement axis increases by a step. The proof-masses are suspended in series with respect to one another by springs in the measurement axis, the stops being arranged to successively intercept the respective proof-masses for increasing thresholds of acceleration.

Abstract: System and method for optical drift correction uses light from a light source that is reflected from a curved surface of a mirror and detected at photosensitive detectors to detect movements of the mirror with respect to the light source.

Abstract: A testing apparatus includes a chip carrying device and a pressing device. The chip carrying device includes a circuit board and a plurality of electrically connecting units that are disposed on the circuit board and each can receive a chip. The pressing device includes a cover and an abutting member disposed between the cover and the electrically connecting units. The cover is disposed on the circuit board to jointly define an accommodating space that accommodates the abutting member and the electrically connecting units. The cover can be connected to an air suction apparatus for expelling air in the accommodating space. When the air suction apparatus performs a suction operation to expel the air in the accommodating space, the abutting member is abutted against the electrically connecting units and the chips so as to connect each of the electrically connecting units and the corresponding chip.