Abstract: The present disclosure describes an apparatus of regulating a channel temperature of a field flow fractionator. In an embodiment, the apparatus includes a thermal conducting block including a top surface, and a bottom surface, where the top surface of the block is configured to be in contact with a bottom surface of a bottom plate assembly of a field flow fractionator, where the bottom plate assembly includes a material with high thermal conductivity, a heater attached to the block where the heater is configured to heat the block, a temperature sensor attached to the block, where the sensor is configured to measure a block temperature of the block, a temperature controller configured to measure a channel temperature of a channel of the field flow fractionator and configured to be connected to the heater and to the temperature sensor, and where the block is configured to heat the bottom plate assembly.

Abstract: Methods and systems for the detection and quantification of multiplicity of ionic analytes comprising CrO42?, AsO43?, SeO42?, and ClO4?, and optionally F?, Cl?, NO2?, NO3?, SO42?, using ion chromatography and suppressed ion conductivity. The method comprises loading a sample loop with a sample; injecting the sample from the sample loop into a column with an eluent, wherein the column comprises a guard column and an analytical column; separating, with the column, the injected sample at an effective separation temperature the injected sample in the presence of an organic modifier into a multiplicity of detectable ionic analytes; suppressing, with a suppressor, background signal; and detecting, with a detector, the multiplicity of ionic analytes.

Abstract: An embodiment pertains to a method for evaluating efficacy of a drug which increases or decreases the secretion of a particular neurotransmitter, by measuring a concentration change of the particular neurotransmitter in a specific intracerebral site with reference to a brain map, the method comprising the steps of: selecting as a microdialysis target region in the brain map a first site of an animal, which corresponds to a site that the brain map represents as being the highest in the concentration of a first neurotransmitter of which the secretion is increased or decreased by the drug; and injecting the drug to the animal and monitoring a concentration change of the first neurotransmitter in the first site between pre- and post-injection of the drug.

Abstract: An odor measurement apparatus includes an odor sensor detecting an odor and an imaging device having a lens portion, in which an imaging direction of the imaging device and an introduction direction of air when the air is guided to a sensor surface of the odor sensor through an introduction port are substantially the same direction. The odor measurement apparatus detects odor substances contained in air using a sensor when an odor is measured, and measures attribute information of a measurement target or the like of the odor. An odor data management apparatus stores and manages odor data measured by the odor measurement apparatus.

Abstract: Systems, devices and methods including a handheld sensing device comprising: a sensor configured to measure ambient methane, ethane, propane, butane, and/or pentane concentrations; and a handle, where the sensor is disposed on a first end of the handle; control electronics comprising: a processor having addressable memory, the processor in communication with the sensor, where the processor is configured to: receive the measured ambient gas concentrations; and detect elevated ambient gas concentrations that may be attributed to a natural gas emissions source based on the measured ambient gas concentrations.

Abstract: In a pathogen identification and remediation system and method, pathogens are identified by capturing samples in an air stream, identifying the pathogens and neutralizing them, with the option of assessing risk levels and issuing alarms when certain risk levels are exceeded.

Abstract: Systems and methods for monitoring the health status of a subject are provided. In certain embodiments, the method includes: applying a sample provided from a subject to a signal enhancing detector configured to indicate an output that is representative of the sample; processing the output with a device configured to acquire the detector output as input data and process the input data to generate a report; and receiving the report. In certain embodiments, the device is a mobile device. In certain embodiments, the method further includes transmitting the sample-derived data in the device to a remote location where the transmitted data is analyzed; and receiving the results of the analysis. Also provided are systems for use in practicing the methods. Kits for use in monitoring the health status of a subject are also provided.

Abstract: A device and method for analyte detection and analytes in a particulate bearing fluid such as whole blood having an instrument for partitioning the panicles from the fluid that is integrated with a detector for analyses of one or more particulate bearing fluid analytes while the particles in the particulate bearing fluid are partitioned.

Abstract: Pathogen detection includes providing a sensor having first conductive lines alternated with second conductive lines, the first and second lines being formed over a substrate providing electrical isolation between the first and second lines and being at least partially exposed. Via voltage circuitry, a voltage difference is applied between the first and second lines. A user's breath is applied to the sensor and contacts exposed parts of the first lines simultaneous to exposed parts of the second lines. Pathogens from the user's breath bridge the electrical isolation between an individual first line and an opposing, individual second line and cause a short circuit. Via a comparator or controller, a current is detected flowing in the first and second lines due to the short circuit through the pathogens. A warning module indicates that the comparator or controller detected that the current is above a warning threshold.

Abstract: A device, system and method related to a pneumatic system for fluid analysis. The device, system and method comprise a connection interface for a fluid analyzer unit, a connection interface for a pump unit, a flow sensor and a pressure sensor. The device, system and method further comprises a control unit for calculating a pump stroke force or amplitude, and/or pump frequency based on measurements from the flow sensor and the pressure sensor for obtaining a constant flow through the pneumatic system.

Abstract: Approaches provide for machine learning or training algorithms that apply modifications to models based on a type of data obtained, including, for example, producer-specific management practice data, genetic data, among other such data. The animal-centric models can be configured to, for example, quantify gas emissions (e.g., greenhouse gas emissions) that an animal may be expected to emit over a period of time, including, for example, over the animal's lifetime. The emissions in certain embodiments can further enable the certification of emissions for individual animals.

Abstract: An apparatus for measuring hemolysis in a cartridge based automated blood analyzer is described. The apparatus allows hemolysis testing to be performed on a sample which is presented as a whole blood sample for other testing by the cartridge based automated blood analyzer. A disposable module is configured for optically analyzing one or more plasma analytes in a flow cell while red blood cells are acoustically separated from plasma in the flow cell.

Abstract: Systems and methods for characterizing cancer cells are disclosed. In certain embodiments, the systems and methods involve coating a first chamber with ECM material and adding a first plurality of cells to the first chamber, adding media to a second chamber, and adding a second plurality of cells and media to a third chamber. The first, second, and third chambers are then clipped together to form a chamber array. The chamber array is mounted to an electric cell impedance sensing reader and impedance readouts of cell invasion are collected from the electric array at time intervals. Cells detected to invade into the second chamber are extracted and characterized.

Abstract: Provided are a method and a technique for kinetic analysis of a compound such as a drug, using a cell. Specifically, disclosed is a method for kinetic analysis of a compound using a cell. The method includes a CE step of feeding a first buffer solution to a container containing a target compound and housing a cell, adjusting and keeping the internal temperature of the container to a first temperature, and adjusting the internal temperature of the container to a second temperature lower than the first temperature. Amounts S and S? of the compound in the first buffer solution are measured at a time interval t1 in the CE step, and based on the amounts S and S? and the time interval t1, an excretion rate of the compound excreted from a basal/basolateral face of the cell is determined.

Abstract: There is provided a microfluidic device comprising: a microfluidic device for preparing a cell model, comprising: a housing having at least three layers; a first inlet area at a top layer and at one end of the housing for receiving a first mixture comprising cells; a second inlet area at the top layer and at an opposite end of the housing for receiving a second mixture containing one or more agents or one or more pathogens; and a plurality of microchannels through which the first mixture and/or the second mixture flows into corresponding wells, wherein each microchannel has an end in fluid communication with the first inlet area, and another end in fluid communication with the second inlet area. There are also provided methods comprising using the device.

Abstract: Disclosed are a cell analyzer and a method for classifying white blood cells based on an impedance method. The method includes: adding a sample to be analyzed to a white blood cell counting chamber; adding a hemolytic agent to the white blood cell counting chamber at least once; controlling the temperature of the liquid in the white blood cell counting pool to be within a predetermined range; and analyzing the liquid in the white blood cell counting chamber to classify white blood cells into at least four classifications and counting.

Abstract: The present disclosure provided a blood cell analyzer, a control device and a blood analysis method thereof. In the method, a first reagent is mixed with a sample to obtain a first testing sample, and then a second reagent is mixed with the first testing sample for a further reaction to get a second testing sample for basophil classification and/or HGB measurement. A blood sample may be tested in one reaction cell through time-division multiplexing technology to obtain four groups leukocytes classification result and HGB result by single detection channel. Thus, the structure of the analyzer may be greatly simplified on the premise of guaranteeing the performance of the analyzer, the size and cost of the analyzer may reduce and a performance-price ratio of the analyzer may increase.

Abstract: The present invention provides a magnetic-control measurement system, comprises a reaction container and a programable magnetron measurement unit.

Abstract: A method of making a nanocomposite includes forming at least one gold nanorod; coating a silver layer on an outer surface of the gold nanorod; assembling a Raman reporter molecule layer on the coated silver layer; coating a pegylated layer on the assembled Raman reporter molecule layer; and conjugating the coated pegylated layer with an active layer, the active layer comprising at least one of a targeting molecule configured to bind to the target of interest and a functional molecule configured to interact with the target of interest.

Abstract: The present disclosure relates to a system for viral monitoring in effluent, comprising a biosensor including a graphene-based field-effect transistor having capture proteins conjugated thereto, the capture proteins being configured to bind a virus, and a fluidic channel arranged above the graphene-based field-effect transistor so that fluid of the effluent flows over the graphene-based field-effect transistor, and processing circuitry configured to apply a gate voltage to the graphene-based field-effect transistor, measure a conductance across the graphene-based field-effect transistor, the conductance reflecting an amount of the virus bound to the capture proteins, compare the measured conductance to a threshold conductance, and transmit, to a computing device and when the comparison indicates the measured conductance satisfies the threshold conductance, information indicating a presence of the virus in the effluent. In certain embodiments, the capture proteins may be SARS-CoV-2 spike antibodies.

Abstract: A system for viral monitoring in effluent includes at least one graphene-based field-effect transistor and circuitry, wherein the circuitry is configured to repeatedly monitor and determine presence of SARS-CoV-2 (COVID virus) in the effluent. The circuitry is configured to apply a gate voltage and measure a conductance across each of the at least one graphene-based field-effect transistor, compare the measured conductance across each of the at least one graphene-based field-effect transistor to a threshold conductance, and determine whether levels of the COVID virus exceed a predetermined threshold in the effluent. If levels of the COVID virus exceed the predetermined threshold in the effluent, the circuitry is configured to remove at least a portion of the bound COVID virus from the proteins of the at least one graphene-based field-effect transistor.

Abstract: Aspects of the disclosure relate to compositions and methods for amplifying and/or detecting one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens), and use of a trap region or reagent to reduce the level of one or more target nucleic acid analytes in an amplified sample prior to or in conjunction with detection. In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. In some embodiments, the methods comprise isothermal amplification of a target nucleic acid and subsequent reduction in the level of one or more target nucleic acid analytes and detection of the amplification products.

Abstract: The present invention provides a method for increasing the sensitivity of LFIAs by using palladium nanoparticles, selecting appropriate dye chemistries, and improving the timing of the development chemistry. In the presence of a palladium nanoparticle, three reagents interact with a catalytic label to form a colored dye. The three reagents include a hydrogen peroxide source, a color developer (a substituted para-phenylenediamine), and a color coupler (e.g. a napthol or a phenol). The timing of the development chemistry is improved by any combination of using a reducing agent, delaying hydrogen peroxide application by diffusion, using dissolving materials as a time delay, using serpentine flow, and separating the color coupler and the color developer on the strip.

Abstract: A detection component for blood group antigens comprising a test strip and a sample addition device, and a sample is transported to a testing section of the test strip by a sampling ring of the sample addition device. Immunochromatography technology is used to identify human blood group antigens with existing blood group antibodies. The antibodies are pre-coated with and solidified on the reaction membrane to define testing points and then assembled to the flushing pad, and the water absorption paper and the pad to define the test strip.

Abstract: A method for determining cleavage of a VAMP by a clostridial neurotoxin in a cell that has been contacted with the clostridial neurotoxin under conditions suitable for clostridial neurotoxin activity, the method comprising contacting the cytoplasmic content of the cell with an antibody that binds to a resulting C-terminal product of such cleavage under suitable conditions in vitro or ex vivo and detecting the binding of the antibody to the C-terminal cleavage product. The antibody may, for example, be capable of binding an antigenic polypeptide consisting of 10 to 65 amino acid residues and comprising an epitope comprising an amino acid sequence that is at least 90% identical to an amino acid sequence of at least 8 amino acid residues that is immediately C-terminal to a clostridial neurotoxin cleavage site in the VAMP.

Abstract: Therapeutic treatments of a tumor expressing pT346 PDK1, including glioma expressing pT346 PDK1, are disclosed.

Abstract: Disclosed herein are methods of treating and making prognostic prediction of, monitoring of therapeutic outcome for treatment of cervical carcinoma in a patient in need thereof by quantifying gene expression in a sample, wherein the genes include 10 high risk genes; calculating the subject's survival risk score by determining the protein expression levels and their relationships using machine learning (ML) and artificial intelligence. The survival risk category of a patient is determined by the consensus or plurality voting of a large number of ML models that individually have excellent predictive potential, thus providing a very robust prognostic biomarker for cervical carcinoma.

Abstract: Presented herein are systems and methods for detecting biomolecular cargo [e.g., protein, e.g., DNA, e.g., RNA (e.g., microRNA, e.g., non-coding RNA), e.g., dyes, e.g., aptamers] inside of particles (e.g., exosomes, e.g., viruses, e.g., extracellular vesicles) contained in complex biological samples (e.g., cells, e.g., tissues, e.g., human blood, plasma, and/or serum).

Abstract: Various embodiments can provide a method of amplifying a fluorescence signal through the cyclic staining of complementary antibodies conjugated with fluorophores. According to various embodiments, the method of amplifying a fluorescence signal may be configured to prepare a primary antibody and antibody pairs with respect to each target protein, bind the primary antibody to the target protein, and bind the antibody pairs to the primary antibody. The multicolor fluorescence signals may be amplified and fabricated by forming fluorescence signals in a way to prepare different antibodies for which cross reactions with different types of target proteins, respectively, have been removed using an agarose gel and to bind different antibody pairs according to combinations of different antibodies to different primary antibodies bound to target proteins, respectively.

Abstract: Methods of obtaining and kits that can be used to obtain an optical super-resolution image of a sample or a portion thereof or an individual or a portion thereof. In various examples, the individual is an individual with cancer. In various examples, a method includes contacting a sample or individual with one or more aluminosilicate nanoparticle(s) that have at least one organic fluorophore molecule covalently bonded to the aluminosilicate network of the nanoparticle(s), or a composition including the aluminosilicate nanoparticle(s); irradiating the sample or the individual, thereby exciting at least one of the fluorophore molecules of an individual aluminosilicate nanoparticle; and obtaining a fluorescence image or a sequence of fluorescence images, which can be processed to obtain a super-resolution image of the sample or the individual. In various examples, the sample is a biological sample, living or fixed tissues and/or cells, or a biopsy obtained from an individual.

Abstract: Biomarkers tests which can be used to predict a positive or negative risk of preeclampsia are described. More specifically, a panel of biomarkers including MMP-7 and gpIIbIIIa, described. The test is useful to predict preeclampsia when a biological sample is obtained between the 16th and 22nd week of pregnancy. Prediction later in pregnancy can be achieved by a combination of Siglec-6, Activin A, ALCAM, and/or FCN2.

Abstract: The present disclosure pertains to method and system of characterizing a protein using an electrospray ionization source.

Abstract: The present disclosure relates in some aspects to methods and compositions for assessing the target binding capacity of a binding molecule, such as an antigen binding molecule, e.g., a monoclonal antibody. In some embodiments, provided herein is a method of analyzing binding of a barcode-labeled antibody to a target antigen expressed by a cell, wherein cells expressing the candidate antigens are partitioned and wherein detection of the barcoded nucleic acid molecule is indicative of the binding between the antibody or antigen-binding fragment thereof and the candidate antigen or epitope of the cell.

Abstract: The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using as says that detect Chitinase-3-like protein 1 as diagnostic and prognostic biomarker assays in renal injuries.

Abstract: The present invention is focused on an in vitro method for the diagnosis or prognosis of neurodegenerative disorders. The method comprises measuring the level of expression or the concentration level of at least a lipoprotein receptor-related protein fragment (LRP fragment) or at least a lipoprotein receptor-related protein (LRP).

Abstract: The present invention identifies biomarkers that are diagnostic of nerve cell injury and/or neuronal disorders. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of nerve injury, the cell(s) involved in the injury, and the subcellular localization of the injury.

Abstract: Assays for rapid measurement of total vitamin D in blood are provided. Vitamin D is measured following the rapid and irreversible release of vitamin D due to denaturation and digestion of vitamin D binding proteins by aspartyl peptidases (e.g., pepsin) under acidic conditions. Such measurements may be made using a vitamin D binder (e.g., an antibody) to measure competition between free vitamin D and added, labeled vitamin D. Synergy between denaturation and degradation is believed to provide more rapid and more complete release of vitamin D than would occur with acid or enzyme alone. These measurements may be made using small amounts of whole blood, serum, or plasma, and are suitable for use in automated devices. These methods provide the advantages of reduced cost, increased speed, reduced discomfort to the subject, and increased availability and ease of use. Reagents, kits, devices, and systems for these assays are also disclosed.

Abstract: A physiological signal monitoring device is adapted for monitoring a physiological signal of a biofluid, and includes: a biosensor strip that has at least one signal output end adapted for outputting the physiological signal; a strip reciprocating module that includes a strip seat for receiving the biosensor strip, a guide seat mounted to the strip seat, and a rotating plate mounted rotatably to the strip seat for triggering reciprocating movement of the biosensor strip and the guide seat relative to the strip seat; and a contact module that includes an electronic module, and at least one extending piece connected electrically with the at least one signal output end to transmit the physiological signal to the electronic module.

Abstract: A test strip code reader is provided. The test strip code reader includes a test strip carrier for accommodating a test strip, a first conductive element for decoding a code of the test strip, a second conductive element configured to be in a contact state or a separation state with the first conductive element, and an activation element having a body and a plurality of protrusions, wherein the body is used to prevent the first conductive element from being contaminated, and according to whether the plurality of protrusions are actuated by the test strip, the contact state or the separation state between the first conductive element and the second conductive element is determined to decode the code of the test strip.

Abstract: A liquid distribution method comprises: providing at least two ferrying units, each ferrying unit made to reciprocate between an initial workstation and a first workstation; transferring from the initial workstation onto each ferry unit a reactor containing a sample; at the first workstation, adding a reagent into the reactor; recording, as a first cycle, a shortest time window during which a sequence of actions performed by each ferry unit can be cyclically reproduced, and recording, as a second cycle, a quotient obtained by dividing the first cycle by the number of ferry unit, and successively transferring the reactors to other ferry units staggeredly at intervals of the second cycle; and sequentially removing from the ferry units the reactors for which the mixing has been completed, staggeredly at intervals of the second cycle, and placing another reactor containing the sample onto the ferry unit from which the reactor was removed.

Abstract: The conveying system includes a support guide element defining a guide track, the support guide element being configured to receive a containers support and guide said containers support in translation along the guide track; and a self-propelled conveying carriage displaceable along a conveying track extending along the support guide element, the self-propelled conveying carriage comprising a drive element movably mounted between at least one drive position in which the drive element is configured to transmit a drive movement to the containers support so received on the support guide element, and a release position in which the drive element is configured to release the containers support, the self-propelled conveying carriage being configured to displace the containers support in translation along the guide track when the drive element is in the drive position and the self-propelled conveying carriage is displaced along the conveying track.

Abstract: There is provided an automated analysis device capable of transferring a rack to a predetermined instrument extraction position efficiently and rapidly, the rack being loaded with instruments to be used for an analysis process. An automated analysis device of the invention includes a conveyance unit to raise and lower a plurality of racks in a vertically stacked state to convey the racks into an analyte processing space, the racks being loaded with instruments to be used for analyte processing. The conveyance unit includes a movement mechanism to move an uppermost rack from a rack standby position to an extraction position with guidance of a protrusion and a recess while maintaining a state of slidable protrusion and recess engagement between the uppermost rack and a rack below the uppermost rack.

Abstract: A reagent container lid opening and closing mechanism capable of opening and closing a reagent container lid, and an automated analysis device. A reagent container lid opening and closing mechanism includes an opening and closing operation body being movable, and including an opening and closing engagement portion to engage engagement portions provided in upper surfaces of container lids of reagent containers. The opening and closing operation body is movable along a longitudinal axis direction of the opening and closing operation body between a retreated position where the opening and closing operation body is separated from the reagent containers, and an engaged position where an opening and closing engagement portion of the opening and closing operation body engages the engagement portions of the container lids. The opening and closing operation body is movable along a predetermined circular arc trajectory between the engaged position and a lid opening position.

Abstract: There is provided an automated analysis device capable of transferring a reagent for reagent dispensing efficiently and rapidly. An automated analysis device of the invention includes a reagent transfer unit to transfer a reagent suction nozzle between a reaction unit and a reagent supply unit. In a region of an analyte processing space where a tip supply unit, the reagent supply unit, a tip disposal unit, and the reaction unit are lined up along a straight line, the reagent transfer unit forms a uniaxial transfer line where the reagent suction nozzle is moved only in a uniaxial direction along the straight line for a reagent transfer.

Abstract: An automatic analyzer includes a cleaning pool, a discharge port, a waste fluid pipe, a valve, and a guiding unit. The cleaning pool is used to clean a predetermined member with a fluid. The discharge port discharges the fluid. The waste fluid pipe connects the cleaning pool and the discharge port. The valve is disposed to the waste fluid pipe. The guiding unit guides a cleaning fluid to the cleaning pool via the waste fluid pipe.

Abstract: A method of washing an aspiration probe of an in-vitro diagnostic system is disclosed. The aspiration probe comprises an outer surface and an inner surface forming an inner space for receiving a fluid. The method comprises dipping the aspiration probe into a first wash fluid so that the outer surface is immersed at least in part into the first wash fluid, aspirating an amount of the first wash fluid into the inner space of the aspiration probe, propagating an ultrasonic vibration to the outer surface of the aspiration probe via the first wash fluid, and rinsing the outer surface and the inner surface of the aspiration probe with a second wash fluid. Further, an in-vitro diagnostic method and an in-vitro diagnostic system are disclosed.

Abstract: There is provided an automated analysis device capable of transferring instruments to be used for an analysis process to a predetermined processing position efficiently and rapidly. In an automated analysis device of the invention, a transfer unit forming a transfer mechanism to transfer instruments to be used for analyte processing into an analyte processing space includes a holding portion to hold the instruments. The holding portion includes a first fitting attachment portion to be attachable to a first instrument in a fitted state, and a second fitting attachment portion to be attachable to a second instrument in a fitted state.

Abstract: A method for calibrating linear vibration and angular vibration based on monocular vision. A motion sequence image of a feature mark is acquired by an industrial camera, and an output signal of a linear and angular accelerometer is acquired by a data acquisition card, where the feature mark and the linear and angular accelerometer are fixed on a work table. An exciting acceleration of the linear and angular accelerometer is measured by a monocular vision method integrating a camera calibration method and a sub-pixel edge extraction method based on line segment detection. The exciting acceleration and the output signal are fitted by a sine approximation method, respectively, and corresponding fitted peaks are obtained. A sensitivity of the linear and angular accelerometer is obtained according to the fitted peaks.

Abstract: The present invention provides a probe card comprising a probe base, at least one impedance-matching probes, and a plurality of first probes. The probe base has a probing side and a tester side opposite to the probing side. Each impedance-matching probe has a probing part and a signal transmitting part electrically coupled to the probing part, wherein one end of the signal transmitting part is arranged at tester side, and the signal transmitting part has a central probing axis. Each first probe has a probing tip and a cantilever part coupled to the probing tip, wherein the cantilever part is coupled to the probe base and has a first central axis such that an included angle is formed between the central probing axis and the first central axis.

Abstract: The invention relates to a signal analyzer, comprising a signal receiving unit configured to receive a signal, in particular a radio frequency (RF) signal, a digitizing unit configured to digitize the received signal, and a trigger detection unit configured to detect a trigger event in the digitized signal. The signal analyzer further comprises an acquisition unit configured to store a segment of the digitized signal in a memory of the signal analyzer if the trigger detection unit detects the trigger event in the digitized signal, and an anomaly search unit configured to analyze the stored segment of the digitized signal in order to detect signal anomalies, in particular glitches, in the stored segment of the digitized signal.