Patents Issued in August 1, 2023
  • Patent number: 11713446
    Abstract: The present invention provides improved and/or shortened processes and methods for reprogramming TILs in order to prepare therapeutic populations of TILs with increased therapeutic efficacy. Such reprogrammed TILs find use in therapeutic treatment regimens.
    Type: Grant
    Filed: May 4, 2022
    Date of Patent: August 1, 2023
    Assignee: IOVANCE BIOTHERAPEUTICS, INC.
    Inventors: Cecile Chartier-Courtaud, Krit Ritthipichai
  • Patent number: 11713447
    Abstract: The invention is directed to production of megakaryocyte (MK) compositions and their the treatment of thrombocytopenia in a subject in need thereof.
    Type: Grant
    Filed: August 4, 2017
    Date of Patent: August 1, 2023
    Assignee: Icahn School of Medicine at Mount Sinai
    Inventors: Camelia Iancu-Rubin, Ronald Hoffman
  • Patent number: 11713448
    Abstract: Methods for producing hepatocytes from pluripotent human stem cells are disclosed herein. The stem cells are plated on a cell culture substrate comprising two laminins. The stem cells are then exposed to different cell culture mediums to induce differentiation. The resulting hepatocytes have higher metabolic capacity compared to hepatocytes cultured on different substrates.
    Type: Grant
    Filed: June 16, 2020
    Date of Patent: August 1, 2023
    Assignee: BIOLAMINA AB
    Inventors: Louise Kristina Hagbard, Carl Gunnar Jesper Ericsson, Katherine Rachel Cameron, David Colin Hay, Stuart John Forbes, Hassan Rashidi
  • Patent number: 11713449
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Grant
    Filed: April 21, 2021
    Date of Patent: August 1, 2023
    Assignee: Ths Trustees of the University of Pennsylvania
    Inventors: Martin Lock, Mauricio Alvira
  • Patent number: 11713450
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Grant
    Filed: May 24, 2021
    Date of Patent: August 1, 2023
    Assignee: Ths Trustees of the University of Pennsylvania
    Inventors: Martin Lock, Mauricio Alvira
  • Patent number: 11713451
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
    Type: Grant
    Filed: October 29, 2020
    Date of Patent: August 1, 2023
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
  • Patent number: 11713452
    Abstract: Certain embodiments are directed to modified or variant Cas9 proteins, and/or methods of using the same.
    Type: Grant
    Filed: September 10, 2018
    Date of Patent: August 1, 2023
    Assignee: UNIVERSITY OF NORTH TEXAS HEALTH SCIENCE CENTER
    Inventors: Jin Liu, Zhicheng Zuo, Yu-Chieh Wang
  • Patent number: 11713453
    Abstract: The invention concerns fusion proteins, wherein two endoglycosidases are fused, possibly via a linker. The fusion enzymes according to the invention have structure (1): EndoX-(L)p-EndoY (1), wherein EndoX is an endoglycosidase, EndoY is an endoglycosidase distinct from EndoX, L is a linker and p is 0 or 1. Such fusion enzymes capable of trimming glycoproteins comprising at least two distinct glycoforms in a single step. The invention further concerns the use of the fusion enzyme according to the invention for trimming glycoproteins. In another aspect, the invention relates to the process of production of the fusion enzyme. In a further aspect, the inventions concerns a process for trimming glycoproteins, comprising trimming the glycoprotein with a fusion enzyme according to the invention, to obtain a trimmed glycoprotein.
    Type: Grant
    Filed: December 7, 2020
    Date of Patent: August 1, 2023
    Assignee: SYNAFFIX B.V.
    Inventors: Remon Van Geel, Maria Antonia Wijdeven, Inge Catharina Josephina Hurkmans, Floris Louis Van Delft, Sander Sebastiaan Van Berkel
  • Patent number: 11713454
    Abstract: The present invention provides a mutated histidine decarboxylase suitable for a practical use. Specifically, the present invention provides a mutated histidine decarboxylase having at least one amino acid residue mutated as compared to a wild-type histidine decarboxylase, and having higher histidine decarboxylase activity and/or stability than the wild-type histidine decarboxylase, and also a use thereof. The mutated histidine decarboxylase has Motifs (1) to (6), and an amino acid residue in at least one motif thereof can be mutated. The mutated histidine decarboxylase can also have a mutation of at least one amino acid residue in an amino acid sequence designated by SEQ ID NO: 3 and in a homologous sequence thereto.
    Type: Grant
    Filed: September 10, 2019
    Date of Patent: August 1, 2023
    Assignee: AJINOMOTO CO., INC.
    Inventors: Hiroki Yamaguchi, Masayuki Sugiki, Kunio Nakata
  • Patent number: 11713455
    Abstract: For enhanced selection of efficient targeted genome manipulating agents, an apparatus includes first and second chip-based biosensors having one or more sensing surfaces configured to detect biomolecular binding interactions between a nucleic acid sample and a targeted genome manipulating agent functionalized to a capture surface within a sensing range of the one or more sensing surfaces. The first chip-based biosensor uses a nucleic acid sample incubated with a blocking agent that blocks on-target binding and the second chip-based biosensor holds a nucleic acid sample that omits the blocking agent. A measurement apparatus measures first and second sets of response signals produced in response to the biomolecular binding interactions occurring between the nucleic acid sample and the targeted genome manipulating agent. An analysis module determines the genome manipulating efficiency parameters of the targeted genome manipulating agent. A system and a method perform the functions of the apparatus.
    Type: Grant
    Filed: February 5, 2020
    Date of Patent: August 1, 2023
    Assignee: Cardea Bio, Inc.
    Inventors: Regis Peytavi, Kiana Aran, Brett Goldsmith, Alexander Kane
  • Patent number: 11713456
    Abstract: The invention comprises methods for isolating nucleic acids, such as DNA and RNA, while enzyme-inhibiting polyanions are reduced at the same time, using a non-alcoholic binder solution that is not based on chaotropic salts, and a washing solution containing an amine compound, and kits suitable for such a method, comprising the mentioned binder solution and washing solution.
    Type: Grant
    Filed: April 8, 2021
    Date of Patent: August 1, 2023
    Assignee: AXAGARIUS GMBH & CO. KG
    Inventor: Andreas von Bohl
  • Patent number: 11713457
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: November 14, 2022
    Date of Patent: August 1, 2023
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov
  • Patent number: 11713458
    Abstract: The present invention relates to a method of modifying at least one gene in a cell via CRISPR/Cas, wherein the at least one gene has at least three alleles. The present invention further relates to cells obtainable by the method of the invention. Additionally, the present invention provides a method of producing a protein in a cell obtainable by the method of modifying at least one gene of the invention. Moreover, the invention relates to proteins obtainable by the method of producing a protein and use thereof, for example in therapy.
    Type: Grant
    Filed: June 20, 2017
    Date of Patent: August 1, 2023
    Assignee: Octapharma AG
    Inventors: Karina Nawrath, Janine Guendel, Sven Bahrke, Lars Stoeckl, Steffen Goletz
  • Patent number: 11713459
    Abstract: Aspects of the invention described herein concern the incorporation of a FOXP3 cDNA (e.g., full-length human codon-optimized cDNA) into a FOXP3 gene or a non-FOXP3 locus so as to provide constitutive or regulated FOXP3 expression in a primary human CD34+ cells or cells derived from edited CD34+ cells. In some embodiments, guide RNA sequences that are directed to FOXP3, AAVS1, or other candidate loci are used for CRISPR/Cas9-mediated gene regulation, and gene delivery cassettes for HDR based gene-modification are provided.
    Type: Grant
    Filed: April 25, 2019
    Date of Patent: August 1, 2023
    Assignee: SEATTLE CHILDREN'S HOSPITAL
    Inventors: David J. Rawlings, Iram F. Khan, Yuchi Chiang Honaker, Swati Singh, Karen Sommer, Andrew M. Scharenberg
  • Patent number: 11713460
    Abstract: This invention is in the field of molecular biology, gene expression, functional genomics, and bioinformatics and relates to novel RNA and related structures and methods of use thereof that enables modulation of gene expression and preservation of particular transcriptome targets. The invention contemplates various applications of RNA sequences derived from the genomic RNA of flaviviruses (FVs) and the application of such features in combination with heterologous sequences.
    Type: Grant
    Filed: February 11, 2021
    Date of Patent: August 1, 2023
    Assignee: The Regents of the University of Colorado
    Inventors: Jeffrey S. Kieft, Erich G. Chapman, David A. Costantino, Jay R. Hesselberth, Andrea MacFadden, Benjamin Akiyama
  • Patent number: 11713461
    Abstract: The present disclosure provides methods of treating patients having decreased bone mineral density, methods of identifying subjects having increased risk of developing decreased bone mineral density, methods of detecting human Zinc And Ring Finger 3 (ZNRF3) variant nucleic acid molecules and variant polypeptides, and ZNRF3 variant nucleic acid molecules and variant polypeptides.
    Type: Grant
    Filed: June 22, 2020
    Date of Patent: August 1, 2023
    Assignees: Regeneran Pharmaceuticals, Inc., University of Maryland, Baltimore
    Inventors: Da-Wei Gong, Nehal Gosalia, Alan Shuldiner, Cristopher Van Hout, James Perry
  • Patent number: 11713462
    Abstract: Provided herein are methods, compounds, and compositions for reducing expression of GYS1 in an individual. Such methods, compounds, and compositions are useful to treat, prevent, delay, or ameliorate a glycogen storage disease or disorder in an individual in need.
    Type: Grant
    Filed: December 23, 2021
    Date of Patent: August 1, 2023
    Assignees: Ionis Pharmaceuticals, Inc., The Hospital for Sick Children
    Inventors: Tamar R. Grossman, Susan M. Freier, Berge Minassian, Saija Ahonen
  • Patent number: 11713463
    Abstract: Described herein are compositions and methods that are used to increase the expression of SCN2A, which may be used to treat neurological or psychiatric disorders. Antisense oligonucleotides that target upstream open reading frames (uORFs) may be administered to prevent translation initiation from a uORF to increase expression from a primary ORF (pORF), thus increasing the levels of SCN2A protein.
    Type: Grant
    Filed: January 17, 2019
    Date of Patent: August 1, 2023
    Assignee: The Florey Institute of Neuroscience and Mental Health
    Inventors: Steven Petrou, Eric G. Marcusson
  • Patent number: 11713464
    Abstract: Provided herein are compositions including aptamers capable of binding to and/or inhibiting the activity of nucleolin. Methods of treating cancer in a subject by administering such compositions are also provided.
    Type: Grant
    Filed: September 10, 2018
    Date of Patent: August 1, 2023
    Assignee: Duke University
    Inventors: Bruce A. Sullenger, Michael Goldstein, Elizabeth D. Pratico, Michael Kastan, Bethany Gray
  • Patent number: 11713465
    Abstract: The present invention provides methods for the transformation of viable explants from wheat seeds to permit production of transgenic wheat plants. The present invention also relates to methods for producing such explants and related embodiments.
    Type: Grant
    Filed: November 21, 2014
    Date of Patent: August 1, 2023
    Assignee: MONSANTO TECHNOLOGY, LLC
    Inventors: David R. Duncan, Sarah Heifner, David Kelm, Brian J. Martinell, Lorena Moeller, Anatoly Rivlin, Rebecca Rode, Xudong Ye, Ashok Shrawat, Yurong Chen
  • Patent number: 11713466
    Abstract: Methods are provided to mutate, in a targeted manner, the genome of a plant cell using a double stranded DNA break inducing enzyme. Also provided are plants, in particular Brassica plants that yield seeds producing oils having a reduced total saturated fatty acid content, and method for making such plants.
    Type: Grant
    Filed: November 20, 2018
    Date of Patent: August 1, 2023
    Inventor: Meridith Kammerzell
  • Patent number: 11713467
    Abstract: Provided are plasmid vector constructs encoding multiple immunomodulatory proteins where each protein or component thereof can be expressed utilizing appropriate promotors and/or translation modifiers. Additional immunomodulatory proteins and genetic adjuvants containing shared tumor antigens can be added to further therapeutic potential as well as allow tracking of therapeutic treatment. Also provides are methods of expressing the plasmid constructs.
    Type: Grant
    Filed: December 16, 2016
    Date of Patent: August 1, 2023
    Assignee: OncoSec Medical Incorporated
    Inventors: Jean Campbell, David A. Canton, Robert H. Pierce
  • Patent number: 11713468
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel elements that further improve expression. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Grant
    Filed: August 2, 2018
    Date of Patent: August 1, 2023
    Assignee: DNA TWOPOINTO INC.
    Inventors: Jeremy Minshull, Maggie Lee
  • Patent number: 11713469
    Abstract: Provided herein are adenoviral vectors comprising nucleotide sequences encoding a Zika virus M and Env antigen, wherein the nucleotide sequence encoding the Zika virus M and Env antigen is operably linked to a cytomegalovirus (CMV) promoter comprising at least one tetracycline operator (TetO) motif. Also provided herein are pharmaceutical compositions comprising the adenoviral vectors, methods of producing the adenoviral vectors, methods of preventing Zika virus or the progression of Zika virus in a subject in need thereof, and kits comprising the adenoviral vectors and host cells.
    Type: Grant
    Filed: July 19, 2019
    Date of Patent: August 1, 2023
    Assignee: Janssen Vaccines & Prevention B.V.
    Inventor: Taco Gilles Uil
  • Patent number: 11713470
    Abstract: Provided herein are nucleic acids for expressing modified ligand-gated ion channel proteins in excitable cells or secretory cells, such as nerves and neurons and optionally including viral sequences, such as Adeno-associated virus sequences, for delivery to excitable cells or secretory cells of a patient. Also provided herein are methods of modulating cell membrane potentials in an excitable cell or secretory cell, and for treatment of a disease or disorder associated with the nervous system in a patient, such as chronic pain or itch.
    Type: Grant
    Filed: March 20, 2018
    Date of Patent: August 1, 2023
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Rebecca Seal, Cynthia Mary Arokiaraj
  • Patent number: 11713471
    Abstract: Described herein are methods, compositions, and systems derived from uncultivated microorganisms useful for gene editing.
    Type: Grant
    Filed: March 28, 2022
    Date of Patent: August 1, 2023
    Assignee: METAGENOMI, INC.
    Inventors: Brian Thomas, Christopher Brown, Audra Devoto, Cristina Butterfield, Lisa Alexander, Daniela S. A. Goltsman, Justine Albers, Alan Brooks, Greg Cost, Morayma Temoche-Diaz, Cindy Castelle, Rebecca Lamothe
  • Patent number: 11713472
    Abstract: Methods of RNA-guided DNA endonuclease-mediated genome editing in Bacteria and Archaea are provided.
    Type: Grant
    Filed: March 4, 2019
    Date of Patent: August 1, 2023
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: William W. Metcalf, Dipti Dinkar Nayak
  • Patent number: 11713473
    Abstract: The present disclosure discloses a process for accelerating, increasing, and stabilizing production of biogas with a high methane content in systems for biodigestion of organic waste. The process comprises: a) obtaining from an anaerobic biodigester (3) a fraction of organic waste equal in weight to K times the weight of the daily organic waste load of the anaerobic biodigester (3), wherein the parameter K is a numeric value selected between 10?3 and 10?1, sending the fraction to one or more acceleration devices (4), retaining the fraction in the one or more acceleration devices (4) until a final concentration (Cf) of the methanogenic bacteria in the fraction is equal to M times an initial concentration (Ci), wherein the parameter M is a numeric value selected between 103 and 108; b) as soon as said final concentration (Cf) is obtained in the fraction, directing said fraction from the acceleration device (4) back to the anaerobic biodigester (3); and c) successively repeating steps a) and b).
    Type: Grant
    Filed: July 1, 2021
    Date of Patent: August 1, 2023
    Assignees: G-Meta Consultoria, Participaçôes e Serviços LTDA, Bio-Tronic Energy Co. LLC
    Inventor: Luciano Pesce
  • Patent number: 11713474
    Abstract: Processes and systems for recovering products from a fermentation mash. In some examples, a process for recovering products from a fermentation mash can include processing a ground corn product to produce a fermentation mash that can include ethanol. At least a portion of the ethanol can be separated from the fermentation mash to produce a whole stillage. The whole stillage can be separated to produce a fiber rich product and a filtrate. The fiber rich product can be hydrolyzed to produce a saccharification mash. The saccharification mash can be processed to produce additional ethanol and a stillage protein product.
    Type: Grant
    Filed: February 18, 2022
    Date of Patent: August 1, 2023
    Assignee: POET GRAIN (OCTANE), LLC
    Inventor: Jason Bootsma
  • Patent number: 11713475
    Abstract: Disclosed are genetically engineered microbial cells for the production of oligosaccharides comprising a galactose-?1,4-glucose moiety at their reducing end, wherein said microbial cells are able to produce said oligosaccharides in the absence of exogenously added lactose, and a method of producing said oligosaccharides using said microbial cells.
    Type: Grant
    Filed: September 2, 2019
    Date of Patent: August 1, 2023
    Assignee: Chr. Hansen HMO GmbH
    Inventors: Stefan Jennewein, Dirk Wartenberg, Katja Parschat
  • Patent number: 11713476
    Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.
    Type: Grant
    Filed: July 12, 2021
    Date of Patent: August 1, 2023
    Assignee: NOVOZYMES, INC.
    Inventors: Brett McBrayer, Tarana Shaghasi, Elena Vlasenko
  • Patent number: 11713477
    Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of ?-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.
    Type: Grant
    Filed: August 24, 2021
    Date of Patent: August 1, 2023
    Assignee: Syngenta Participations AG
    Inventor: George W. Aux
  • Patent number: 11713478
    Abstract: The present disclosure relates to methods for characterizing and diagnosing dental diseases. In particular, the present disclosure relates to methods for characterizing and diagnosing dental disease (e.g., gingivitis and periodontal disease) based on levels of AI-2 in oral fluids and plaque.
    Type: Grant
    Filed: August 31, 2020
    Date of Patent: August 1, 2023
    Assignees: THE REGENTS OF THE UNIVERSITY OF MICHIGAN, COLGATE-PALMOLIVE COMPANY
    Inventors: Alexander Rickard, William Giannobile, Michelle Swetky, Nielson T. Baxter, Steven E. Miller, Laurence Du-Thumm, Hans Stettler
  • Patent number: 11713479
    Abstract: A method for determining whether a test sample contains a phytopathogenic fungus, which includes (a) putting the test sample on a front surface of a substrate having a through hole; the substrate having a cellulose film on the back surface thereof; the through hole has a cross-sectional area of not less than 7.065 square micrometers and not more than 19.625 square micrometers; and the cellulose film has a thickness of not less than 0.5 micrometers and not more than 3.7 micrometers; (b) leaving the test sample at rest after the step (a); (c) irradiating the cellulose film with ultraviolet light after the step (b); (d) bringing a back surface of the cellulose film into contact with a fungus color reaction reagent after the step (c); and (e) determining that the test sample contains the phytopathogenic fungus, if a color is given to the fungus color reaction reagent.
    Type: Grant
    Filed: July 16, 2021
    Date of Patent: August 1, 2023
    Assignee: PANASONIC INTRILECTUAL PROPERTY MANAGEMENT CO., LTD.
    Inventors: Yuki Kushida, Yoshitsugu Uriu, Yosifumi Kariatumari
  • Patent number: 11713480
    Abstract: The present disclosure relates to materials and methods for spatial detection of nucleic acid in a tissue sample or a portion thereof. In particular, provided herein are materials and methods for detecting RNA so as to obtain spatial information about the localization, distribution or expression of genes in a tissue sample. In some embodiments, the materials and methods provided herein enable detection of gene expression in a single cell.
    Type: Grant
    Filed: March 30, 2022
    Date of Patent: August 1, 2023
    Assignee: The Regents of the University of Michigan
    Inventor: Jun Hee Lee
  • Patent number: 11713481
    Abstract: A process for pre-concentration and detection of at least one single-stranded nucleic acid target molecule, the process comprising the steps of generating a flow of liquid comprising at least one magnetic nanoparticle in a micro-channel and a plurality of single-stranded nucleic acid probe molecules attached to the nanoparticle, generating an alternating magnetic field in the part of the micro-channel using an electromagnet, the magnetic field having an intensity and frequency that are suitable for causing magnetic hyperthermia of the nanoparticles so as to cause denaturing of the duplex formed by the single-stranded nucleic acid target molecule and the single-stranded nucleic acid probe molecule, and detecting the single-stranded target molecule dispersed in the liquid.
    Type: Grant
    Filed: October 5, 2018
    Date of Patent: August 1, 2023
    Assignees: Centre National de la Recherche Scientifique (CNRS), Institut National de La Sante et de la Recherche Medicale (INSERM), Sorbonne Universite
    Inventors: Marie-Charlotte Horny, Jean-Michel Siaugue, Vincent Dupuis, Mathieu Lazerges, Jean Gamby
  • Patent number: 11713482
    Abstract: Disclosed herein are methods, devices and compositions comprising nucleic acid captor molecules with a stem region and a loop region for detecting target nucleic acids.
    Type: Grant
    Filed: June 15, 2017
    Date of Patent: August 1, 2023
    Assignee: GENECAPTURE, INC.
    Inventors: Paula M. Koelle, Krishnan Chittur, Valentin Korman, Zachary McGee
  • Patent number: 11713483
    Abstract: Provided herein are products and methods for detecting analytes using polymers that bind to such analytes and thereby undergo a conformational change or contribute to a newly formed complex.
    Type: Grant
    Filed: February 8, 2017
    Date of Patent: August 1, 2023
    Assignee: Children's Medical Center Corporation
    Inventors: Wesley Philip Wong, Clinton H. Hansen
  • Patent number: 11713484
    Abstract: Providing herein, among other things, is a method comprising incubating a double-stranded nucleic acid having a nick with a nick translating activity, a ligase, and a nucleotide mix comprising at least one modified nucleotide, to generate a product comprising a patch of a newly synthesized strand of a duplex nucleic acid containing a plurality of modified nucleoside monophosphates that are at or adjacent to the site of the nick. In some embodiments, the method may be used to map damaged nucleoside monophosphates in a nucleic acid. Compositions and kits for use in performing the method are also provided.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: August 1, 2023
    Inventors: Kelly M. Zatopek, Vladimir Potapov, Jennifer Ong, Laurence Ettwiller, Lixin Chen, Thomas C. Evans, Jr., Andrew F. Gardner
  • Patent number: 11713485
    Abstract: The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample.
    Type: Grant
    Filed: August 3, 2021
    Date of Patent: August 1, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Evan R. Daugharthy, George M. Church
  • Patent number: 11713486
    Abstract: The present invention provides combinations of biomarkers that can be used in the diagnosis and differentiation of bipolar disorder and schizophrenia. The present invention therefore provides methods of differentiating, diagnosing and treating bipolar disorder and schizophrenia, by examining relevant proteins and RNA in a patient sample.
    Type: Grant
    Filed: September 15, 2017
    Date of Patent: August 1, 2023
    Assignees: The Regents of the University of California, Laguna Diagnostics, LLC
    Inventors: Terry W. Osborn, Marquis Vawter
  • Patent number: 11713487
    Abstract: In the field of transplant rejection, identified are SNPs wherein mismatches in variants present in a recipient and donor for such SNPs are predictive of transplant outcome, wherein the SNPs represent non-HLA loci newly implicated in rejection. By the invention, transplant outcomes such as elevated risk of antibody mediated rejection, elevated risk of T-cell mediated rejection, or low risk of rejection can be predicted by analyzing mismatches between donor and recipient for the enumerated SNPs. Certain SNPs enumerated are predictive of kidney transplant outcome. The compatibility of prospective donors can be assessed for a recipient, allowing for optimized donor-recipient pairing.
    Type: Grant
    Filed: July 16, 2018
    Date of Patent: August 1, 2023
    Assignee: The Regents of the University of California
    Inventors: Minnie Sarwal, Marina Sirota, Silvia San Juan Pineda
  • Patent number: 11713488
    Abstract: The present invention generally relates to the field of cancer, in particular to cancers having microsatellite instability (MSI) and/or mismatch repair (MMR-) deficiency. Examples of such cancers include many colorectal, gastric, and endometrial tumors. Accordingly, the present invention provides a novel diagnostic marker panel for analyzing MSI loci, together with methods and kits of using said panel in the detection of cancers having microsatellite instability (MSI) and/or mismatch repair (MMR-) deficiency.
    Type: Grant
    Filed: January 22, 2019
    Date of Patent: August 1, 2023
    Assignee: BIOCARTIS NV
    Inventors: Bram De Craene, Klaas Decanniere, Jan Van De Velde, Geert Maertens
  • Patent number: 11713489
    Abstract: Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles. During the first phase, fluorescence probes do not anneal to template strands and do not emit fluorescence signals. During a second phase of the processing step, the thermocycler may heat the reaction mixture to a third temperature and cool the reaction mixture to a fourth temperature repeatedly for a second plurality of cycles.
    Type: Grant
    Filed: October 26, 2020
    Date of Patent: August 1, 2023
    Assignee: Tetracore, Inc.
    Inventors: William M. Nelson, Kyle Armantrout, Tracy Calvin Fecteau
  • Patent number: 11713490
    Abstract: A method for identifying a quantitative trait locus associated with desirable nutritional traits in canola includes: analyzing a population of canola plants or germplasm for desirable nutritional traits; determining the genotype of the canola plants or germplasm using at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111; mapping the canola plants or germplasm for the presence of a quantitative trait locus (QTL) associated with the markers; and associating the QTL with the desirable nutritional trait. An isolated and/or recombinant nucleic acid includes a sequence associated with a quantitative trait locus (QTL), wherein the QTL is associated with a desirable nutritional trait in a canola plant or germplasm and wherein the QTL is further associated with at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111.
    Type: Grant
    Filed: October 6, 2020
    Date of Patent: August 1, 2023
    Assignee: CORTEVA AGRISCIENCE LLC
    Inventors: Shunxue Tang, Van L. Ripley, Thomas G. Patterson, Michelle Wiggins, Joshua A. Flook, Cherie Ochsenfeld, Daniel Garcia, Syed Masood Rizvi, Ryan Preuss, Donna Carolynn Knievel, Zoe Christina Ehlert, Steve Rounsley, Muhammad Tahir
  • Patent number: 11713491
    Abstract: Provided herein is a nucleotide cassette comprising an inducible promoter, a nucleotide sequence that corresponds to at least one single stranded RNA diagnostic target, a nucleotide sequence that encodes artemin, a molecular switch and a nucleotide sequence that encodes a DNAse enzyme and is under control of the molecular switch, wherein the single stranded RNA diagnostic target is a sequence detected by a molecular diagnostic assay. In some embodiments the nucleotide cassette can be used to obtain an RNA expression product. Also provided are vectors and cells comprising the nucleotide cassette or the RNA expression product thereof. The nucleotide cassette can further be used to obtain a diagnostic control composition comprising a non-pathogenic recombinant bacterium having a modified genetic content comprising the nucleotide cassette and to methods of producing such recombinant bacteria.
    Type: Grant
    Filed: April 23, 2021
    Date of Patent: August 1, 2023
    Assignee: UNIVERSITY OF THE WITWATERSRAND, JOHANNESBURG
    Inventors: Bavesh Davandara Kana, Edith Erika Machowski
  • Patent number: 11713492
    Abstract: A roller chain having at least one pair of offset links wherein the offset link plates are made with a steel having a high chromium content and are through-hardened using an austempering heat treatment (such as a salt bath quench). The resulting offset link plates may have a hardness in range of 44-50 HRC on the Rockwell hardness scale and a bainite metallurgical microstructure. The offset link plates may also have a greater fatigue strength than at least one of the inner link plates and the outer link plates. The inner and outer link plates may be formed out of a plain carbon steel which is heated, quenched and tempered to produce a martensite microstructure.
    Type: Grant
    Filed: May 20, 2020
    Date of Patent: August 1, 2023
    Assignee: Iwis Drive Systems, LLC
    Inventor: Andrew J. Binford
  • Patent number: 11713493
    Abstract: This disclosure provides engineered microbes coding at least one rare earth element (REE) binding ligand for the preferential separation of REEs, as well as methods of use thereof.
    Type: Grant
    Filed: February 28, 2019
    Date of Patent: August 1, 2023
    Assignee: LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
    Inventors: Dan Mcfarland Park, Aaron William Brewer, Yongqin Jiao
  • Patent number: 11713494
    Abstract: Provided are processes for extracting lithium and optionally nickel from a Nickel(II)/Lithium(I) (Ni2+/Li+) solution. The extraction is optionally performed in a series of steps with counterflow of aqueous and organic flows to thereby produce a lithium poor solution. The lithium poor solution may be treated so that remaining Ni in the lithium poor solution may be directly precipitated therefrom in the form of a Ni salt. Once complete, the process provides for recoverable nickel and/or lithium that may be recycled into batteries or sold for other uses.
    Type: Grant
    Filed: November 4, 2020
    Date of Patent: August 1, 2023
    Assignee: BASF Corporation
    Inventors: Jack Bender, William C. Mays, Tinoush Dinn, Martin L. Panchula, Dieter G. Von Deak
  • Patent number: 11713495
    Abstract: A tantalum or tantalum alloy which contains pure or substantially pure tantalum and at least one metal element selected from the group consisting of Ru, Rh, Pd, Os, Ir, Pt, Mo, W and Re to form a tantalum alloy that is resistant to aqueous corrosion. The invention also relates to the process of preparing the tantalum alloy.
    Type: Grant
    Filed: April 12, 2021
    Date of Patent: August 1, 2023
    Assignee: Materion Newton Inc.
    Inventors: Paul R. Aimone, Evan Hinshaw