With Analysis Or Detailed Detection Patents (Class 204/461)
  • Patent number: 10794802
    Abstract: In various embodiments, the present application teaches methods for tissue clearing in which tissues are rendered macromolecule-permeable and optically-transparent, thereby exposing their cellular structure with intact connectivity. In some embodiments, the present application teaches ePACT, which is a protocol for enhanced tissue clearing via expansion. In some embodiments, the present application teaches visualizing a tissue that has been expanded via ePACT.
    Type: Grant
    Filed: August 17, 2016
    Date of Patent: October 6, 2020
    Assignee: California Institute of Technology
    Inventors: Viviana Gradinaru, Jennifer Treweek
  • Patent number: 10697869
    Abstract: Provided herein are systems, devices, and methods for improved treatment of tissue, such as brain tissue. The improved treatment described herein can result in improved tissue penetration of various compounds and chemicals, such as stains and immunohistochemistry reagents. For example, provided herein is a pressurizing device that may include a chamber body having an opening in one of a top and a sidewall of the body, and may also include a chamber lid covering the opening and releasably coupled to the chamber body proximate the opening. The chamber lid and chamber body form an air-tight cavity. The pressurizing device may also have an inlet passing through one of the chamber body and the chamber lid and into the air-tight cavity. The device may also include a retainer coupled inside the air-tight cavity and configured to releasably couple to at least one tissue sample receptacles.
    Type: Grant
    Filed: April 29, 2019
    Date of Patent: June 30, 2020
    Assignee: Dignity Health
    Inventors: Roberto Fiorelli, Gurpaul Singh Sidhu
  • Patent number: 10641731
    Abstract: An electrophoresis running tank assembly uses two opposed rows of LEDs to illuminate DNA-containing gel on a transparent tray positioned between the rows. A respective cylindrical lens is positioned horizontally between each row and a respective edge of the tray. The optical axis of the illumination light is midway between a bottom surface of the gel tray and a top surface of the gel.
    Type: Grant
    Filed: October 24, 2017
    Date of Patent: May 5, 2020
    Assignee: C.C. IMEX
    Inventors: Winston Glenn Walker, Richard Chan, Rita M. Wong, Ramey K. Chan, Po Ling Cheng
  • Patent number: 10559404
    Abstract: It is disclosed a power cable comprising at least one conductor and a hollow tube at least partially filled with a traceable material. The traceable material comprises a tracer associated with a uniquely identifiable code and is in a liquid or gel form. The tracer may comprise one or more of: coded synthetic DNA particles, a fingerprint of one or more trace materials, microdots containing a code written thereon. The tracer may also comprise radio-frequency identification, RFID, tags.
    Type: Grant
    Filed: June 8, 2017
    Date of Patent: February 11, 2020
    Assignee: PRYSMIAN S.p.A.
    Inventors: Andreas Neumann, Frank Lübbe
  • Patent number: 10495554
    Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.
    Type: Grant
    Filed: May 25, 2017
    Date of Patent: December 3, 2019
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Karl A. Deisseroth, William E. Allen, Brian Hsueh, Li Ye
  • Patent number: 10473658
    Abstract: The invention relates to a family of compounds that comprise fluorescent cyanine dyes. The compounds are near infrared absorbing heptamethine cyanine dyes with a 4,4-disubstituted cyclohexyl ring as part of the polymethine chromophore. The compounds are generally hydrophilic and can be chemically linked to biomolecules, such as proteins, nucleic acids, and therapeutic small molecules. The compounds can be used for imaging in a variety of medical, biological and diagnostic applications.
    Type: Grant
    Filed: February 7, 2018
    Date of Patent: November 12, 2019
    Assignee: VisEn Medical, Inc.
    Inventor: Narasimhachari Narayanan
  • Patent number: 10082453
    Abstract: An airborne microbial measurement apparatus and a measurement method thereof are provided. An airborne microbial measurement apparatus according to an embodiment includes a discharge apparatus including a discharge electrode and a voltage supply unit applying a high voltage to the discharge electrode. A substrate is provided to a side of the discharge apparatus to collect an airborne microbe from air by a high voltage applied to the discharge electrode. A reagent injection apparatus supplies a dyeing reagent to the microbe collected on the substrate or a DNA of the microbe. A light emission measurement apparatus senses a quantity of light generated from the DNA to which the dyeing reagent is supplied. The discharge apparatus includes a controller controlling the voltage supply unit so that the voltage is applied to collect the airborne microbe or destroy an external wall of the collected airborne microbe.
    Type: Grant
    Filed: September 19, 2014
    Date of Patent: September 25, 2018
    Assignees: LG ELECTRONICS INC., NATIONAL UNIVERSITY CORPORATION TOYOHASHI UNIVERSITY OF TECHNOLOGY
    Inventors: Chulwoo Park, Sunghwa Lee, Akira Mizuno, Kazunori Takashima, Hirofumi Kurita, Hachiro Yasuda
  • Patent number: 10043054
    Abstract: Methods, systems, and devices for classifying a biological sample that include receiving an image of a biological sample and applying one or more algorithms from a data repository to the image, generating a classification of the biological sample based on the outcome of the one or more algorithms applied to the image, and transmitting the classification for presentation on a display or via another medium. The methods, systems, and devices may also include features for developing a data master reference and/or other correlation/translation features to enable comparison of data sets from one platform to another or from one machine to another or from the same machine at different points in time.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: August 7, 2018
    Assignee: CIRECA THERANOSTICS, LLC
    Inventors: Stanley H. Remiszewski, Max Diem, Clay M. Thompson, Aysegul Ergin, Xinying Mu, Mark Kon
  • Patent number: 9897604
    Abstract: The invention relates to a family of compounds that comprise fluorescent cyanine dyes. The compounds are near infrared absorbing heptamethine cyanine dyes with a 4,4-disubstituted cyclohexyl ring as part of the polymethine chromophore. The compounds are generally hydrophilic and can be chemically linked to biomolecules, such as proteins, nucleic acids, and therapeutic small molecules. The compounds can be used for imaging in a variety of medical, biological and diagnostic applications.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: February 20, 2018
    Assignee: VisEn Medical, Inc.
    Inventor: Narasimhachari Narayanan
  • Patent number: 9752178
    Abstract: Methods and compositions for determining the nucleic acid sequence of polynucleotides that are at least 1500 nucleotides in length are provided.
    Type: Grant
    Filed: September 26, 2014
    Date of Patent: September 5, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: Chao-ting Wu, George M. Church
  • Patent number: 9663554
    Abstract: Multifunctional molecular weight protein ladders and methods of making thereof are disclosed herein that are useful for determining the molecular weight of a test protein and/or the relative mass or amount of the test protein in a protein separation assay, such as gel electrophoresis or western blotting. Also included are compounds of Formula I (e.g., mono acetylated MP-11 NHS ester) that may be used to label purified proteins of the protein ladder. The MP-11 label protein ladder can be detected on a blotting membrane by exposing the microperoxidase to a suitable substrate, such as a chromogenic substrate or a chemiluminescent substrate.
    Type: Grant
    Filed: September 17, 2015
    Date of Patent: May 30, 2017
    Assignee: LI-COR, Inc.
    Inventors: Nisha Padhye, Rose Skopp, Ying Wang
  • Patent number: 9494552
    Abstract: Disclosed in this specification is a high capacity compact gel documentation system for documenting different types of electrophoresis gels or other translucent objects using ultraviolet light. The system includes a base with a scanning surface having a transparent bottom surface. A light source is connected to a conveying mechanism, disposed below the transparent bottom surface, to move the light source over the length of the transparent bottom surface. An image capture device receives a reflected image and provides it to a microprocessor. Moreover, separate interchangeable filters allow for documentation of UV and white light gel captures, alleviating the need for separate transilluminators and hoods with filters and cameras.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: November 15, 2016
    Assignee: Research Foundation of The City University of New York
    Inventors: Farshad Tamari, Farnaz Tamari
  • Patent number: 9488616
    Abstract: Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment.
    Type: Grant
    Filed: May 17, 2013
    Date of Patent: November 8, 2016
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Christopher Belisle
  • Patent number: 9428806
    Abstract: Systems and methods for specific nucleic acid (NA) sequence detection that do not rely on polymerase chain reaction (PCR) for target sequence amplification and do not require any special reagents other than a complementary sequence capture probe conjugated to spherical beads.
    Type: Grant
    Filed: February 25, 2014
    Date of Patent: August 30, 2016
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Harold G. Monbouquette, Jacob J. Schmidt
  • Patent number: 9279152
    Abstract: Sample preparation processes for in situ RNA or DNA analysis, methods and compositions therefor are provided. Processes provided herein allow DNA or RNA analysis to be carried out in the same tube or on an aliquot of the prepared sample without centrifugation or extraction. The preparation process can be carried out at room temperature in as little as seven minutes and is amenable to high throughput processing using manual or robotic platforms.
    Type: Grant
    Filed: August 14, 2014
    Date of Patent: March 8, 2016
    Assignee: Applied Biosystems, LLC
    Inventors: Richard Fekete, Annalee Nguyen
  • Patent number: 9164039
    Abstract: A method includes calibrating color bleed factors of optical detector channels of a sample processing apparatus through processing a color bleed calibration substance which includes a plurality of different size fragments replicated from different groups of DNA loci, wherein fragments in a same group are labeled with a same fluorescent dye, and fragments in different groups are labeled with different fluorescent dyes having different emission spectra, wherein the different size fragments are processed during different acquisition times.
    Type: Grant
    Filed: October 20, 2010
    Date of Patent: October 20, 2015
    Assignee: Analogic Corporation
    Inventor: Ching Ming Lai
  • Patent number: 9097663
    Abstract: The present teachings provide methods, devices, systems, and materials for performing electrophoresis in an automated fashion. The electrophoresis system may simultaneously image gel during an electrophoresis run. In some embodiments, the electrophoresis system may analyze an imaged gel during or after electrophoresis. The device may comprise a gel processing system, a gel illumination system, an image capture system, and an image analysis all housed within a housing.
    Type: Grant
    Filed: December 17, 2012
    Date of Patent: August 4, 2015
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Timothy Updyke, Paul Predki, Evangeline Gonzalez, Randall Lowe, Tad Simons
  • Publication number: 20150144490
    Abstract: Methods and compositions are provided for preparing a biological specimen for microscopic analysis. These methods find many uses, for example in medicine and research, e.g., to diagnose or monitor disease or graft transplantation, to study healthy or diseased tissue, to screen candidate agents for toxicity and efficacy in disease modification. Also provided are reagents, devices, kits and systems thereof that find use in practicing the subject methods.
    Type: Application
    Filed: March 13, 2013
    Publication date: May 28, 2015
    Applicant: The Board of Trustees of the Leland Standford Junior University
    Inventors: Karl A. Deisseroth, Kwanghun Chung
  • Patent number: 9005418
    Abstract: Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.
    Type: Grant
    Filed: August 7, 2013
    Date of Patent: April 14, 2015
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Christopher Belisle
  • Publication number: 20150083594
    Abstract: An electrophoresis controller for use with an electrophoresis apparatus having a gel matrix disposed between electrodes for separation of particles along with a tracking dye. The electrophoresis controller includes a sensor system and a controller. The sensor system includes a support, a light emitter, and a light receiver. The support includes a first portion positionable on a first side of the gel matrix and a second portion positionable adjacent a second side of the gel matrix. The light emitter is positioned on the first portion of the support for emitting light onto one side of the gel matrix. The light receiver is positioned on the second portion of the support adjacent to the other side of the gel matrix for receiving light from the light source as it is passing through the gel matrix. At least one of the light emitter and the light receiver includes a light guide having a first end and a second end.
    Type: Application
    Filed: October 22, 2014
    Publication date: March 26, 2015
    Applicant: THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK
    Inventors: Papa Nii ASARE-OKAI, Rabi A. MUSAH, Tony P. HOANG
  • Patent number: 8974731
    Abstract: A sample processing apparatus includes a sample carrier receiving region configured to receive a sample carrier. The sample carrier includes at least one sample channel carrying at least one sample, at least one agent chamber carrying at least one agent to be moved to the at least one sample channel to facilitate processing of the at least one sample, and the at least one agent chamber includes at least one chamber cover covering at least one opening of the at least one agent chamber, inhibiting flow of the at least one agent from the at least one agent chamber to the at least one sample channel. The sample processing apparatus further includes a chamber opener configured to facilitate opening the at least one chamber cover. The sample processing apparatus further includes a fluid mover that moves the agent out of the at least one agent chamber after the at least one chamber cover is opened and into the at least one sample channel.
    Type: Grant
    Filed: August 4, 2011
    Date of Patent: March 10, 2015
    Assignee: Analogic Corporation
    Inventor: Ari Eiriksson
  • Patent number: 8974651
    Abstract: The present invention discloses apparatuses and methods for the visualization of fluorophores in biological systems such as electrophoresis gels and cell cultures. Preferred embodiments of the apparatuses consists of a tray or tank having one or more light sources disposed to direct light through an electrophoresis gel disposed within the vessel such that the luminescence from fluorophores in the gel are easily visualized.
    Type: Grant
    Filed: April 17, 2011
    Date of Patent: March 10, 2015
    Assignee: C.C. IMEX
    Inventors: Richard Chan, Winston Glenn Walker, Rita M. Wong, Jason In
  • Publication number: 20150060279
    Abstract: The present embodiments provide systems, kits and methods suitable for performing dry or substantially dry electro-blotting analyses on immobilized protein or nucleic acid samples. Electro-blotting performed according to the presently described embodiments may include a step whereby detection of one or more immobilized proteins or nucleic acids is electrophoretically accelerated. Methods for performing electro-blotting of immobilized proteins or nucleic acids may include applying an electric voltage to one or more reagents typically used in protein or nucleic acid blotting procedure. The one or more reagents may be absorbed on a suitable carrier matrix. Electro-blotting performed in accordance with the systems and methods described herein may be performed under substantially dry conditions (i.e., with little or no aqueous buffers).
    Type: Application
    Filed: August 11, 2014
    Publication date: March 5, 2015
    Inventors: Ilana MARGALIT, Galia Feldman, Svetlana Lifshits
  • Publication number: 20150060280
    Abstract: Jatropha lines can be classified and discriminated by a method including the steps of: conducting a nucleic acid amplification reaction using a primer set including (i) adjacent forward primer and (ii) adjacent reverse primer, and (iii) LTR forward primer or LTR reverse primer, or (iv) RTP forward primer or RTP reverse primer, wherein DNA prepared from Jatropha that is an objective of determination, is used as a template; and determining the presence or absence of insertion of LTR-type retrotransposon including the retrotransposon sequence used in (iii) or (iv), on the basis of the presence or absence and length of an amplification product obtained by the amplification reaction.
    Type: Application
    Filed: August 29, 2014
    Publication date: March 5, 2015
    Inventors: Kiichi FUKUI, Suguru TSUCHIMOTO, Hiroe SAKAI, Atefeh ALIPOUR, Toshifumi HOSOYA, Yasuhisa YUSHIO
  • Publication number: 20150050673
    Abstract: The present invention is directed to a method of detecting intact fibrinogen, comprising the steps of: a) providing a sample containing at least some fibrinogen optionally converted at least in part to fibrin, and optionally containing thrombin; b) solubilizing the sample in a solubilizing solution that inhibits thrombin activity; c) after optional SDS-PAGE transferring/applying a portion of said sample to a protein-binding membrane; d) reacting the fibrinogen with a primary monoclonal antibody capable of binding to fibrinopeptide A moiety; and e) detecting the quantity of intact fibrinogen in the sample by quantifying the amount of the bound primary monoclonal antibody.
    Type: Application
    Filed: October 29, 2014
    Publication date: February 19, 2015
    Inventors: Ashley DeAnglis, Elif Burcoglu
  • Publication number: 20150047980
    Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.
    Type: Application
    Filed: October 28, 2014
    Publication date: February 19, 2015
    Applicant: Caliper Life Sciences, Inc.
    Inventors: Dan Camporese, Advit Bhatt, Josh Molho, Hui Xu, Ken Summers
  • Publication number: 20140311909
    Abstract: Disclosed herein is a protein standard for gel electrophoresis. The standard may be detected using multiple modalities. These modalities include, for example, observation of color or fluorescence arising from dyes, porphyrins, or haloalkylated tryptophan residues covalently linked to proteins of the standard; or detection of fluorescence or chemiluminescence arising from antibodies or other binding partners bound to proteins of the standard through polypeptide epitopes or affinity tags. The protein standard comprises multiple protein sets, each set corresponding to a different gel band, and proteins within a set may be detectable with one or more modalities.
    Type: Application
    Filed: March 13, 2014
    Publication date: October 23, 2014
    Applicant: Bio-Rad Laboratories, Inc
    Inventors: Joseph S. Siino, JR., Dennis C. Yee, Robin Baginski
  • Publication number: 20140202859
    Abstract: Apparatus and methods for size selecting nucleic acid molecules having wide range of applications including the production of DNA libraries for sequencing technologies. An automated high throughput system for size selection of multiple nucleic acid samples that uses imaging technique to detect the progress of a target fraction and feedback from the imaging to control electrophoresis. Predictive algorithms for timed nucleic acid extractions are generated to provide size selected nucleic acid molecules of required size ranges.
    Type: Application
    Filed: June 15, 2012
    Publication date: July 24, 2014
    Applicant: BRITISH COLUMBIA CANCER AGENCY BRANCH
    Inventors: Robin J. Noel Coope, Jared Raymond Slobodan
  • Publication number: 20140186822
    Abstract: Methods for the detection of biologically relevant molecules that comprise concentrating such molecules into microscopic holes in a sheet of chemically inert material, restricting the openings, and measuring the electric current through the holes or the fluorescence near the hole openings. The electric current or fluorescence will change as the molecules diffuse out of the holes, providing a measure of the diffusion rate and thereby detecting the presence and characteristics of the molecules. For molecules that interact, the diffusion rate will be slower than for molecules that do not interact, yielding a determination of the molecular interaction. Capping the population of holes and inserting into a mass spectrometer allows identification of the molecules.
    Type: Application
    Filed: March 4, 2014
    Publication date: July 3, 2014
    Inventor: Christopher Gordon ATWOOD
  • Publication number: 20140076726
    Abstract: The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid.
    Type: Application
    Filed: May 15, 2013
    Publication date: March 20, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Matthew BEAUDET, William Gregory Cox, Stephen Yue
  • Publication number: 20140042027
    Abstract: Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.
    Type: Application
    Filed: August 7, 2013
    Publication date: February 13, 2014
    Applicant: Bio-Rad Laboratories, Inc.
    Inventor: Christopher Belisle
  • Publication number: 20130319864
    Abstract: The invention relates to, among other things, a method for performing electrophoresis with in-situ calibration. The method includes combining a volume of a test sample with a volume or quantity of a calibrating sample to form a final volume, where the volume of the calibrating sample includes a known concentration of a calibrator and the final volume includes a known ratio of test sample to calibrating sample. The method also includes depositing a loading fraction in a receiving well of an electrophoretic gel, in which the loading fraction is a fraction of the final volume, and separating the loading fraction along a common separation lane of the electrophoretic gel such that components of the test sample and the calibrator are separated from one another along the common separation lane.
    Type: Application
    Filed: May 29, 2013
    Publication date: December 5, 2013
    Inventors: Philip GUADAGNO, Erin SUMMERS
  • Publication number: 20130306854
    Abstract: Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment.
    Type: Application
    Filed: May 17, 2013
    Publication date: November 21, 2013
    Inventor: Christopher Belisle
  • Patent number: 8557609
    Abstract: A method employing gel electrophoresis and optical imaging techniques to measure the amount of biomaterial that attaches to specified locations on a detector slide such as a bioarray or biochip.
    Type: Grant
    Filed: October 18, 2010
    Date of Patent: October 15, 2013
    Assignee: Maven Biotechnologies, LLC
    Inventors: Shane Dultz, David Ralin, William Rassman
  • Publication number: 20130210648
    Abstract: Compositions, methods and kits are described for identifying biomolecules (e.g., proteins and nucleic acids) expressed in a biological sample that are associated with the presence, development, or progression of a disease (such as cancer), or more generally determination of the etiology or risk factors associated with a disease. Sample types analyzed by the disclosed methods include but are not limited to archival tissue blocks that have been preserved in a fixative, tissue biopsy samples, tissue microarrays, and so forth. The methods disclosed herein correlate expression profiles of biomolecules with various disease types, and allow for the determination of relative survival rates; in some embodiments, the methods permit determination of survival rates for a subject with cancer. In other embodiments, the disclosure relates to methods for evaluating therapeutic regimes for the treatment, such as treatment of cancer.
    Type: Application
    Filed: March 15, 2013
    Publication date: August 15, 2013
    Inventor: The United States of America, as represented by the Secretary, Department of Health and Human Services
  • Publication number: 20130199930
    Abstract: Disclosed in this specification is a high capacity compact gel documentation system for documenting different types of electrophoresis gels or other translucent objects using ultraviolet light. The system includes a base with a scanning surface having a transparent bottom surface. A light source is connected to a conveying mechanism, disposed below the transparent bottom surface, to move the light source over the length of the transparent bottom surface. An image capture device receives a reflected image and provides it to a microprocessor. Moreover, separate interchangeable filters allow for documentation of UV and white light gel captures, alleviating the need for separate transilluminators and hoods with filters and cameras.
    Type: Application
    Filed: February 7, 2013
    Publication date: August 8, 2013
    Applicant: Research Foundation of The City University of New York
    Inventor: Research Foundation of The City University of New York
  • Publication number: 20130157282
    Abstract: Systems, devices, and methods for accurately imaging chemiluminescence and other luminescence are disclosed. A compact, flat-bed scanner having a light-tight enclosure, one or more detector bars of linear charge-coupled device (CCD) or complementary metal oxide semiconductor (CMOS) imaging chips, and high working numerical aperture (NA) optics scans closely over a sample in one direction and then the opposite direction. Averages or other combinations of intensity readings for each pixel location (x, y) between the two or more passes are averaged together in order to compensate for luminescence that varies over time. On-chip pixel binning and multiple clock frequencies can be used to maximize the signal to noise ratio in a CCD-based scanner.
    Type: Application
    Filed: March 28, 2012
    Publication date: June 20, 2013
    Applicant: Li-Cor, Inc.
    Inventors: Ahmed Bouzid, Chris Lesiak, Daniel W. Konz, David L. Franzen, William Biggs, Jon P. Anderson
  • Publication number: 20130130236
    Abstract: A method for separating labeled cells and a use (of the labeled cells) thereof are provided. More specifically, a method for labeling cells using fluorescent magnetic nanodiamonds, and a method for separating the labeled cells using the labeling method by the fluorescent or magnetic properties of the nanodiamonds are provided.
    Type: Application
    Filed: May 15, 2012
    Publication date: May 23, 2013
    Applicant: NATIONAL CHIAO TUNG UNIVERSITY
    Inventors: Jui-I Chao, Zhi-Yi Lien, Kuo-Chu Hwang
  • Publication number: 20130105320
    Abstract: A slide holder assembly and method for use in high content screening of a comet assay. The slide holder assembly has an inside surface and is configured to receive a slide holding a plurality of biological cells on a top surface thereof. When the slide is secured within the slide holder assembly, the inside surface of the slide holder assembly and the top surface of the slide combine to form a cavity having an open top end. The cavity is watertight and configured to hold a liquid therein. A method of performing a comet assay using a slide disposed within a slide holder assembly. The method includes performing encapsulation, lysis, electrophoresis, staining, and imaging of cells on the slide while the slide remains secured within the slide holder assembly.
    Type: Application
    Filed: October 23, 2012
    Publication date: May 2, 2013
    Applicant: CELLOMICS, INC.
    Inventor: Cellomics, Inc.
  • Patent number: 8431339
    Abstract: The present invention relates to a method for purifying analyte molecules and in particular to a component of this type in which a separation section is used for separating analyte molecules and other constituents of a sample, and in which provision is made of at least one sample chamber for receiving a sample containing the analyte molecules and at least one collecting chamber for receiving the purified analyte molecules. According to the invention, the microfluidic component has at least one integrated receptor device for detecting the presence and/or the concentration of the purified analyte molecules. In accordance with one advantageous development of the present invention, the separation section is formed by an electrophoretic gel filtration section.
    Type: Grant
    Filed: October 26, 2007
    Date of Patent: April 30, 2013
    Assignee: Albert-Ludwigs-Universitat Freiburg
    Inventors: Paul Vulto, Gerald Urban
  • Publication number: 20130053254
    Abstract: The present invention relates to a process for the parallel isolation and/or purification of RNA and DNA from the same fixed biological sample, the quantification and analysis of the nucleic acids isolated by the process according to the invention, to a kit for the parallel isolation and/or purification of RNA and DNA from a fixed sample and to the use of this kit for the diagnosis, prognosis, decision with respect to therapy and/or the monitoring of the therapy of a disease.
    Type: Application
    Filed: February 25, 2011
    Publication date: February 28, 2013
    Applicant: QIAGEN GmbH
    Inventor: Vera Holländer
  • Publication number: 20130032481
    Abstract: A sample processing apparatus includes a sample carrier receiving region configured to receive a sample carrier. The sample carrier includes at least one sample channel carrying at least one sample, at least one agent chamber carrying at least one agent to be moved to the at least one sample channel to facilitate processing of the at least one sample, and the at least one agent chamber includes at least one chamber cover covering at least one opening of the at least one agent chamber, inhibiting flow of the at least one agent from the at least one agent chamber to the at least one sample channel. The sample processing apparatus further includes a chamber opener configured to facilitate opening the at least one chamber cover. The sample processing apparatus further includes a fluid mover that moves the agent out of the at least one agent chamber after the at least one chamber cover is opened and into the at least one sample channel.
    Type: Application
    Filed: August 4, 2011
    Publication date: February 7, 2013
    Applicant: ANALOGIC CORPORATION
    Inventor: Ari Eiriksson
  • Publication number: 20130020199
    Abstract: Electrophoresis systems, assemblies, cassettes and methods for easily, and more effectively and efficiently, isolating a biomolecule band from an electrophoretic gel are provided. The methods use an electrophoresis cassette with at least one loading well and at least one collection well. A sample containing the biomolecule of interest is placed into at least one loading well and buffer or water is placed in at least one collection well. An electric field is then applied to drive migration and separation of the sample into different component bands within the gel. When the component of interest is located within at least one collection well, the electric field is terminated and the buffer or water in the collection well is removed, thereby isolating and collecting the sample component of interest.
    Type: Application
    Filed: July 16, 2012
    Publication date: January 24, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventor: Ilana Margalit
  • Publication number: 20130012604
    Abstract: Methods of predicting the development of medically refractory ulcerative colitis (MR-UC) in a patient by determining the presence or absence of one or more risk variants, where the presence of one or more risk variants is indicative of a severe and/or aggressive form of ulcerative colitis are disclosed.
    Type: Application
    Filed: March 16, 2011
    Publication date: January 10, 2013
    Applicant: CEDARS-SINAI MEDICAL CENTER
    Inventors: Jerome I. Rotter, Kent D. Taylor, Stephan R. Targan
  • Publication number: 20130012602
    Abstract: The present invention relates to prognosing, diagnosing and treating of Crohn's disease.
    Type: Application
    Filed: January 13, 2011
    Publication date: January 10, 2013
    Applicant: CEDARS-SINAI MEDICAL CENTER
    Inventors: Talin Haritunians, Jerome I. Rotter, Kent D. Taylor, Stephan R. Targan
  • Publication number: 20130012402
    Abstract: The invention relates to a method of diagnosing or monitoring schizophrenia or other psychotic disorder.
    Type: Application
    Filed: December 20, 2010
    Publication date: January 10, 2013
    Applicant: Psynova Neurotech Ltd.
    Inventors: Sabine Bahn, Emanuel Schwarz
  • Publication number: 20120328620
    Abstract: Mutations of the epidermal growth factor receptor (EGFr), of phosphatidylinositol 3?-kinase (“PI3K”), and of B-Raf are described. Methods of treating tumors containing mutated EGFr with human monoclonal antibodies against EGFr are described. Methods and kits for ascertaining the presence of one or more mutant EGFr, mutant PI3K, and/or mutant B-Raf in a sample and for treating disorders or conditions related to the presence of mutant EGFr, mutant PI3K, and/or mutant B-Raf are also described. Methods of treating tumors containing mutant EGFr, mutant PI3K, and/or mutant B-Raf are also described.
    Type: Application
    Filed: July 15, 2011
    Publication date: December 27, 2012
    Applicant: AMGEN INC
    Inventors: Daniel J. FREEMAN, Todd JUAN, Robert RADINSKY
  • Publication number: 20120282597
    Abstract: The present invention relates to a biomarker associated with a cancer and a method using the biomarker to evaluate a risk of proliferation, invasion, or metastasis of a cancer. The method of the present invention comprises the following steps: (A) providing a tissue sample to evaluate for risk of proliferation, invasion, or metastasis of a cancer, wherein the tissue sample comprises a non-cancer region, and a suspected cancer region; (B) detecting expression levels of a biomarker and a predetermined standard in the non-cancer region and the suspected cancer region respectively, wherein the biomarker is T-cell lymphoma invasion and metastasis 2 (TIAM2); (C) comparing the expression levels of the biomarker and the predetermined standard in the non-cancer region to the expression levels of the biomarker and the predetermined standard in the suspected cancer region.
    Type: Application
    Filed: May 3, 2011
    Publication date: November 8, 2012
    Applicant: National Cheng Kung University
    Inventors: H. Sunny SUN, Jia-Shing Chen
  • Patent number: 8303790
    Abstract: The invention relates to a protocol for pre-staining a protein prior to electrophoresis, an electrophoresis method including the protocol, and a kit for carrying out the protocol. The protocol comprises the steps of incubating the protein sample with a pyrylium dye in the presence of a buffer, a detergent and a denaturing agent.
    Type: Grant
    Filed: September 12, 2008
    Date of Patent: November 6, 2012
    Assignee: Agilent Technologies, Inc.
    Inventors: Helen McNeill, Kenneth G. Macnamara
  • Publication number: 20120264626
    Abstract: A method for diagnosing and staging of chronic obstructive pulmonary disease (COPD) includes measuring expression of one or more miRNAs levels in a subject suspected of suffering from COPD.
    Type: Application
    Filed: May 7, 2010
    Publication date: October 18, 2012
    Applicant: THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION
    Inventors: Serge Patrick Nana-Sinkam, Philip T. Diaz, Michael E. Ezzie