Blotting Patents (Class 204/464)
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Patent number: 11718874Abstract: The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample.Type: GrantFiled: October 25, 2018Date of Patent: August 8, 2023Assignee: President and Fellows of Harvard CollegeInventors: Evan R. Daugharthy, George M. Church
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Patent number: 11614388Abstract: The present invention provides recovering an extracellular vesicle(s) having high purity from an extracellular vesicle-containing sample. Specifically, the present invention provides a method of recovering an extracellular vesicle(s), comprising: (1) treating an extracellular vesicle-containing sample with a chelating agent; and (2) separating the extracellular vesicle(s) from the extracellular vesicle-containing sample treated with the chelating agent.Type: GrantFiled: October 12, 2017Date of Patent: March 28, 2023Assignee: H.U. GROUP RESEARCH INSTITUTE G.K.Inventors: Tatsutoshi Inuzuka, Ayako Kurimoto
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Patent number: 10191010Abstract: A transfer-membrane retaining jig (2) that retains a transfer membrane (1) in a separation-transfer device (100) includes: a fixing part (20, 21) that fixes at least one end in a movement direction of the transfer membrane (1), in which the fixing part (20, 21) includes an elastic body (20a, 21a) that abuts the transfer membrane (1) from an opposite side to a dispensing part (50a), and a pressing member (20b, 21b) that presses the transfer membrane (1) against the elastic body (20a, 21a).Type: GrantFiled: December 7, 2015Date of Patent: January 29, 2019Assignee: SHARP LIFE SCIENCE CORPORATIONInventors: Takateru Matsunaga, Hideki Kinoshita
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Patent number: 9945809Abstract: Kits, systems, and methods are provided for transferring biological macromolecules from an electrophoresis slab gel to a blotting membrane using conductive polymer electrodes.Type: GrantFiled: February 9, 2016Date of Patent: April 17, 2018Assignee: Bio-Rad Laboratories, Inc.Inventors: Clayton T. McKee, Steve Swihart
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Patent number: 9835623Abstract: A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.Type: GrantFiled: January 15, 2015Date of Patent: December 5, 2017Assignee: Caliper Life Sciences, Inc.Inventors: Andrew Barry, Laurel Provencher, Seth Cohen, I-Jane Chen, Jun Yan, Jingjing Wang
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Patent number: 9657338Abstract: The present invention relates to a method for immobilizing nucleic acids on a support, comprising the provision of a nucleic acid with a stretch of nucleotides of only one basetype and the immobilization of said nucleic acid on a support by crosslinking by light, wherein said crosslinking by light is performed at a wavelength of about 300-500 nm, preferably at a wavelength of 365 nm. The present invention further relates to a method for the analysis of nucleic acids immobilized according to the invention, which comprises the hybridization of the immobilized nucleic acid with complementary and mismatch segments. Furthermore, the present invention relates to immobilized nucleic acids obtainable by the method of the invention, the use of accordingly immobilized nucleic acids for the production of nucleic acid arrays and a diagnostic kit, comprising an array of nucleic acids which are immobilized according to the present invention.Type: GrantFiled: September 22, 2009Date of Patent: May 23, 2017Assignee: Koninklijke Philips N.V.Inventors: Anke Pierik, Hendrik Roelof Stapert
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Patent number: 9612241Abstract: The present disclosure provides an optical readout imaging system may include first electrode, a thin film disposed on the first electrode, a biomolecule transfer layer disposed on the thin film, and a second electrode disposed on the biomolecule transfer layer. The present disclosure also provides a biochemical detection method using the optical readout imaging system.Type: GrantFiled: December 19, 2014Date of Patent: April 4, 2017Assignees: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE, CHANG GUNG MEMORIAL HOSPITAL, LINKOUInventors: Pai-chien Chou, Bo-Wen Xiao, Ming-Hua Yeh, Sin-Huei Wu
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Patent number: 9291596Abstract: A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.Type: GrantFiled: February 12, 2013Date of Patent: March 22, 2016Assignee: Pierce Biotechnology, Inc.Inventors: Gregory John Kilmer, Brian David Wolf, Scott Eugene Starwalt, Gary G Judd, Brian Lynn Webb
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Patent number: 9150611Abstract: The present invention relates to an aqueous transfer buffer that provides superior efficiency in transferring polypeptides of a broad range of molecular weight from a matrix used in electrophoresis to another immobilized surface. Also disclosed are electrophoretic methods and devices in which the aqueous transfer solution of this invention is used.Type: GrantFiled: July 11, 2013Date of Patent: October 6, 2015Assignee: Bio-Rad Laboratories, Inc.Inventors: Nikolas Chmiel, Cory Panattoni
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Publication number: 20150060279Abstract: The present embodiments provide systems, kits and methods suitable for performing dry or substantially dry electro-blotting analyses on immobilized protein or nucleic acid samples. Electro-blotting performed according to the presently described embodiments may include a step whereby detection of one or more immobilized proteins or nucleic acids is electrophoretically accelerated. Methods for performing electro-blotting of immobilized proteins or nucleic acids may include applying an electric voltage to one or more reagents typically used in protein or nucleic acid blotting procedure. The one or more reagents may be absorbed on a suitable carrier matrix. Electro-blotting performed in accordance with the systems and methods described herein may be performed under substantially dry conditions (i.e., with little or no aqueous buffers).Type: ApplicationFiled: August 11, 2014Publication date: March 5, 2015Inventors: Ilana MARGALIT, Galia Feldman, Svetlana Lifshits
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Publication number: 20140339084Abstract: Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes.Type: ApplicationFiled: March 28, 2014Publication date: November 20, 2014Applicant: BIO-RAD LABORATORIES, INC.Inventors: Matthew Latham, William Strong, Nikolas Chmiel, Jeff Xu, Robert Iovanni
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Publication number: 20140224655Abstract: A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.Type: ApplicationFiled: February 12, 2013Publication date: August 14, 2014Inventors: Gregory John Kilmer, Brian David Wolf, Scott Eugene Starwalt, Gary G. Judd, Brian Lynn Webb
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Publication number: 20140131205Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: ApplicationFiled: January 17, 2014Publication date: May 15, 2014Applicant: LIFE TECHNOLOGIES ISRAEL, LTD.Inventors: Ilana MARGALIT, Uri YOGEV, Itay SELA, Yuri KATZ, Adam SARTIEL, Timothy UPDYKE
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Patent number: 8721860Abstract: A technique for producing multiple protein blots from a single gel, including entering the number ‘n’ membranes to be blotted into microprocessor memory, determining calibration constants for a gel batch, inputting calibration constants into microprocessor memory, and calculating ‘n’ voltage/time profiles for simultaneous blotting. A gel layer from the gel batch is treated with a protein sample, ‘n’ membranes are placed onto the gel layer to yield a gel pack, and the gel pack is placed between an electrode plate maintained at a fixed first voltage and an array of ‘n’ spaced generally parallel electrodes. The voltage to each respective ‘n’ spaced generally parallel electrodes is varied over time according to a respective ‘n’ voltage/time profile to transfer proteins to each respective ‘n’ membranes to yield blotted membranes to yield ‘n’ respective blotted membranes.Type: GrantFiled: October 26, 2011Date of Patent: May 13, 2014Assignee: Indiana University Research and Technology CorporationInventors: Stanley Yung-Ping Chien, Hiroki Yokota
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Patent number: 8715476Abstract: Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes.Type: GrantFiled: December 8, 2010Date of Patent: May 6, 2014Assignee: Bio-Rad Laboratories, Inc.Inventors: Matthew Latham, William Strong, Nikolas Chmiel, Jeff Xu, Robert Iovanni
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Patent number: 8702950Abstract: A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.Type: GrantFiled: September 10, 2012Date of Patent: April 22, 2014Assignees: Sharp Kabushiki Kaisha, Toppan Printing Co., Ltd.Inventors: Yuji Maruo, Yutaka Unuma, Atsunori Hiratsuka, Hideki Kinoshita, Kenji Yokoyama, Koji Sakairi, Satonari Akutsu
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Publication number: 20130140183Abstract: The disclosed device for trapping biologically-relevant substances can easily collect biologically-relevant substances from a thin tissue section or a gel in which said biologically-relevant substance have been fractionated. Said device is provided with: trapping bodies for trapping biologically-relevant substances; carriers that hold the trapping bodies; and electrodes for charging the trapping bodies. The trapping bodies are charged and brought into contact with a prescribed position on a thin tissue section that contains biologically-relevant substances or a gel in which biologically-relevant substances have been fractionated, thereby trapping said biologically-relevant substances from said gel or thin tissue section.Type: ApplicationFiled: June 22, 2011Publication date: June 6, 2013Inventor: Hideji Tajima
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Patent number: 8449748Abstract: A sample separation/adsorption appliance (100) according to one embodiment of the present invention includes: a first buffer solution tank (103) equipped with a first electrode (101) and used for holding a first buffer solution; a second buffer solution tank (104) equipped with a second electrode (102) and used for holding a second buffer solution; a sample separation section (110) that holds a sample separation medium (131) for separating a sample; and a conductive section (120) that holds a conductive medium (133), wherein: the sample separation medium (131) and the conductive medium (133) are in contact with the sample adsorption member (132), respectively, on the opposite sides thereof; an end of the sample separation medium (131) opposite to an end being in contact with the sample adsorption member (132) is connected to an inside of the first buffer solution tank (103); and an end of the conductive medium (133) opposite to an end being in contact with the sample adsorption member (132) is connected to anType: GrantFiled: June 1, 2009Date of Patent: May 28, 2013Assignees: Sharp Kabushiki Kaisha, National Institute of Advanced Industrial Science and TechnologyInventors: Uichi Midorikawa, Takateru Matsunaga, Yuji Maruo, Yutaka Unuma, Atsunori Hiratsuka, Hideki Kinoshita, Kenji Yokoyama
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Publication number: 20130130282Abstract: The present invention is directed to a method of detecting intact fibrinogen, comprising the steps of: a) providing a sample containing at least some fibrinogen optionally converted at least in part to fibrin, and optionally containing thrombin; b) solubilizing the sample in a solubilizing solution that inhibits thrombin activity; c) after optional SDS-PAGE transferring/applying a portion of said sample to a protein-binding membrane; d) reacting the fibrinogen with a primary monoclonal antibody capable of binding to fibrinopeptide A moiety; and e) detecting the quantity of intact fibrinogen in the sample by quantifying the amount of the bound primary monoclonal antibody.Type: ApplicationFiled: November 21, 2011Publication date: May 23, 2013Inventors: Ashley DeAnglis, Elif Burcoglu
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Patent number: 8444839Abstract: Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester or polyester/cellulose blend wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.Type: GrantFiled: December 12, 2011Date of Patent: May 21, 2013Assignee: Bio-Rad Laboratories, Inc.Inventors: Xuemei Yang, Cory M. Panattoni
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Patent number: 8394250Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: GrantFiled: February 10, 2012Date of Patent: March 12, 2013Assignee: Life Technologies CorporationInventors: Ilana Margalit, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel
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Patent number: 8357278Abstract: An electrotransfer cassette is formed in two parts that are releasably joined by a manually operated locking mechanism. The joined parts have electrical contact areas extending from the electrodes in the two parts of the cassette, the contact areas being exposed on an outer edge of the resulting cassette to form electrical connections to a power supply upon the simple insertion of the cassette into an instrument.Type: GrantFiled: December 8, 2010Date of Patent: January 22, 2013Assignee: Bio-Rad Laboratories, Inc.Inventor: Matthew Latham
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Patent number: 8268149Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: GrantFiled: August 21, 2006Date of Patent: September 18, 2012Assignee: Life Technologies CorporationInventors: Ilana Margalit, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel
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Publication number: 20120211364Abstract: The invention provides an electroblotting system for blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, an ion source juxtaposed with the anode between the anode and the transfer stack, a cathode, and another ion source juxtaposed with the cathode between the cathode and the transfer stack, in which the each ion source is sufficient for electrophoretic transfer. The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: ApplicationFiled: February 15, 2012Publication date: August 23, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Ilana Margalit, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel
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Publication number: 20120138465Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: ApplicationFiled: February 10, 2012Publication date: June 7, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Ilana MARGALIT, Uri YOGEV, Itay SELA, Yuri KATZ, Adam SARTIEL
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Patent number: 8192601Abstract: An electroblotting cassette is formed in three separable parts—an upper plate, a lower plate, and a base that receives both plates, with electrodes mounted on both the upper and lower plates. The cassette accommodates transfer stacks of different thicknesses by its inclusion of a set of raised areas, known as “lands,” on the floor of the base and a set of inverse lands on the underside of the lower electrode plate, the two sets being spatially arranged to either abut each other or be offset from each other, depending on the orientation of the lower plate, thereby allowing the user a choice between two heights of the lower plate within the base and hence two thicknesses of transfer stacks. Other arrangements include those with more than one set of lands on one or both parts to allow for three or more thickness selections, or depressions in place of lands. Finger-operated latches secure the upper plate to the base.Type: GrantFiled: February 19, 2010Date of Patent: June 5, 2012Assignee: Bio-Rad Laboratories, Inc.Inventor: Matthew Latham
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Patent number: 8173002Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: GrantFiled: February 21, 2006Date of Patent: May 8, 2012Assignee: Life Technologies CorporationInventors: Ilana Margalit, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel
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Publication number: 20120094315Abstract: Provided herein are, inter alia, methods for predicting the susceptibility of a subject to a mental or neurodegenerative disorder, the method comprising obtaining one or more biological samples from the subject; determining the levels of one or more biomarkers in the sample, wherein the biomarkers are selected from pyrroles, histamine, methionine adenosyltransferase (MAT) activity, homocysteine, copper and zinc; and comparing the level(s) of the biomarker(s) determined in (b) with the level(s) of said biomarker(s) from one or more control samples, wherein abnormal levels of the one or more biomarkers in the sample(s) from the subject compared to the one or more control samples is predictive of susceptibility of the subject to a mental or neurodegenerative disorder.Type: ApplicationFiled: December 9, 2009Publication date: April 19, 2012Inventor: Stephanie Fryar-Williams
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Publication number: 20120090999Abstract: Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester or polyester/cellulose blend wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.Type: ApplicationFiled: December 12, 2011Publication date: April 19, 2012Applicant: BIO-RAD LABORATORIES, INC.Inventors: Xuemei Yang, Cory M. Panattoni
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Publication number: 20120088251Abstract: The invention involves assays, diagnostics, kits, and assay components for mass spectrometry and other methods to determine levels of glycated CD59 in subjects.Type: ApplicationFiled: December 7, 2011Publication date: April 12, 2012Applicant: President and Fellows of Harvard CollegeInventor: Jose Halperin
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Publication number: 20120087892Abstract: Disclosed herein is a method of detecting metastasis of a tumor in a subject, such as metastasis to a lymph node. The method includes directly determining an amount of desmoglein-3 (DSG-3) protein in a sample from the subject (such as a lymph node sample) and comparing the amount of DSG-3 protein to a control, wherein an increase in the amount of DSG-3 protein in the sample relative to the control indicates metastasis of the tumor to the lymph node. The disclosed methods further include selecting a therapy (for example, surgery, lymph node dissection, radiation therapy, chemotherapy, or a combination of two or more thereof) for the subject based on the amount of DSG-3 protein in the sample from the subject.Type: ApplicationFiled: June 11, 2010Publication date: April 12, 2012Inventors: J. Silvio Gutkind, Vyomesh Patel, Alfredo Molinolo, Timothy D. Veenstra
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Publication number: 20120058901Abstract: Certain aspects of the present invention relate to new diagnostic and prognostic methods involving DEAR1, a gene is located on the short arm of human chromosome 1. Specifically, analysis of expression or structure of this gene for prognosis or recurrence risk assessment is disclosed.Type: ApplicationFiled: February 12, 2010Publication date: March 8, 2012Inventors: Ann M. Killary, Steven T. Lott, Bruce G. Haffty
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Publication number: 20120058078Abstract: A reporter construct contains mammalian INGAP 5?-regulatory region or a fragment thereof, a minimal promoter element from mammalian INGAP or a heterologous promoter, and a reporter gene. The reporter construct can be used to screen for agents which alone or in combination up-regulate or down-regulate reporter gene expression. Alternatively, the reporter construct can be used to screen for agents that bind to the hamster INGAP 5?-regulatory region or a fragment thereof.Type: ApplicationFiled: August 1, 2011Publication date: March 8, 2012Applicant: GMP Endotherapeutics, Inc.Inventors: David A. Taylor-Fishwick, Aaron I. Vinik
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Publication number: 20120046184Abstract: Provided herein is a method for the isolation or removal of a cellular component from a cell that comprises the steps of applying a pulse of nanoparticles to the cell, allowing the nanoparticles to traffic through the cell for a period of time sufficient to allow the nanoparticles locate to and interact with the cellular component to be isolated, and separation of the nanoparticles and isolated cellular component from the cell.Type: ApplicationFiled: February 26, 2010Publication date: February 23, 2012Applicant: UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLINInventors: Kenneth Dawson, Iseult Lynch, Martin Lundqvist, Tommy Cedervall
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Publication number: 20120000781Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: ApplicationFiled: June 29, 2011Publication date: January 5, 2012Applicant: LITE TECHNOLOGIES CORPORATIONInventors: Ilana MARGALIT, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel
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Patent number: 8075755Abstract: Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester or polyester/cellulose blend wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.Type: GrantFiled: December 13, 2007Date of Patent: December 13, 2011Assignee: Bio-Rad Laboratories, Inc.Inventors: Xuemei Yang, Cory M. Panattoni
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Publication number: 20110297544Abstract: An electrotransfer cassette is formed in two parts that are releasably joined by a manually operated locking mechanism. The joined parts have electrical contact areas extending from the electrodes in the two parts of the cassette, the contact areas being exposed on an outer edge of the resulting cassette to form electrical connections to a power supply upon the simple insertion of the cassette into an instrument.Type: ApplicationFiled: December 8, 2010Publication date: December 8, 2011Applicant: BIO-RAD LABORATORIES, INC.Inventor: Matthew Latham
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Publication number: 20110297545Abstract: Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes.Type: ApplicationFiled: December 8, 2010Publication date: December 8, 2011Applicant: BIO-RAD LABORATORIES, INC.Inventors: Matthew Latham, William Strong, Nikolas Chmiel, Jeff Xu, Robert Iovanni
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Publication number: 20100243453Abstract: This invention relates to electrophoresis, in particular pre-cast mini gels with a low fluorescent polymer backing that is not adherent to the gel. Use of this type of small gel simplifies blotting procedures, such as Southern and Western blotting, after electrophoresis.Type: ApplicationFiled: September 24, 2007Publication date: September 30, 2010Applicant: GE Healthcare Bio-Science ABInventors: Bengt Bjellqvist, Sofia Edlund, Jesper Hedberg
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Publication number: 20100240029Abstract: A cholesterol-regulating complex of SIRT1 and LXR and methods of use are disclosed. SIRT1 forms a complex with LXR bound to an LXR element. Methods of forming the complex, identifying an agent that modulates formation of the complex, increasing the ratio of cholesterol bound to high density lipoprotein (HDL) to total cholesterol in the plasma of a mammal, promoting ABCA1-mediated cholesterol efflux from a mammalian cell, treating a subject deemed to have a level of SIRT1 activity that is below normal, assessing whether a candidate substance modulates an LXR-dependent process, and assessing whether a candidate substance modulates an SIRT1-dependent effect of an LXR are disclosed.Type: ApplicationFiled: June 6, 2007Publication date: September 23, 2010Applicant: Massachusetts Institute of TechnologyInventors: Leonard Guarente, Monty Krieger, Xiaoling Li
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Publication number: 20100086956Abstract: A method for detecting HIV infection in a mammal is disclosed. The method contains the steps of isolating exosomes from a urine sample of a mammal and detecting the presence of HIV-specific biomarker in said isolated exosomes. A method for diagnosing a mammal with an HIV-associated disease, in particular, HIV-associated nephropathy is also disclosed.Type: ApplicationFiled: October 2, 2009Publication date: April 8, 2010Applicant: MOREHOUSE SCHOOL OF MEDICINEInventors: Gale W. Newman, Mike Powell, Akins Doherty, Chamberlain Obialo, Claudette Mitchell-Ali, Khalid Bashir, Mafuz Khan
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Patent number: 7495231Abstract: The invention provides an apparatus for producing an image of a global surface of an ion source sample plate that is exterior to an ion source. In general terms, the apparatus contains a sample plate for an ion source, an imaging device (e.g., a CCD or CMOS camera) and an illumination device that is configured to produce a light beam that contacts the sample plate surface to define a grazing angle between the light beam and the sample plate surface. The apparatus may be present at a location that is remote to the ion source.Type: GrantFiled: September 8, 2005Date of Patent: February 24, 2009Assignee: Agilent Technologies, Inc.Inventors: Jean-Luc Truche, Gregor T. Overney, William D. Fisher, Richard P. Tella
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Publication number: 20090026079Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: ApplicationFiled: July 11, 2008Publication date: January 29, 2009Applicant: INVITROGEN CORPORATIONInventors: Ilana MARGALIT, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel, Timothy Updyke
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Patent number: 7435322Abstract: The invention relates to a device and a method for automatically analysing the constituents of at least one analyte, especially by means of gel electrophoresis and/or isoelectric focussing, comprising at least one separating matrix, especially a gel. Each separating matrix is applied on at least one side in a supporting direction S of a supporting element, can be loaded with an analyte, and can be electrically contacted on opposite ends in a separating direction T in order to split the analyte into its constituents.Type: GrantFiled: February 1, 2002Date of Patent: October 14, 2008Inventor: Hartmut Schlichting
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Patent number: 7250097Abstract: An apparatus and method for the sequential electroelution of biomolecules is described, the apparatus comprising a separation medium having an outlet, and a collector having at least a first receptacle and a second receptacle that can be sequentially brought into contact with the outlet of the separation medium by translating the first receptacle and the second receptacle in relation to the outlet of the separation medium, and the method comprising the steps of receiving a first substantially separated molecule in the first receptacle and translating the first receptacle and the second receptacle such that the second receptacle is brought into in contact with the outlet of the separation medium, receiving a second substantially separated molecule in the second receptacle, and repeating said steps to sequentially receive a desired number of substantially separated molecules.Type: GrantFiled: July 9, 2004Date of Patent: July 31, 2007Assignee: The United States of America, Department of Health and Human ServicesInventors: József Antal, Zsuzsanna Buzás, Andreas Chrambach
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Patent number: 7166441Abstract: Polypeptides are electroblotted through a digestion membrane to a composite capture membrane that can be directly analyzed using mass spectrometry. The molecular weights of the fragments generated by the digestion membrane are then used to identify the polypeptide from which they originated. The digestion membrane contains an immobilized protease such as trypsin, which cleaves the electroblotted polypeptides into fragments during electroblotting with such high enzyme cleavage capacity and efficiency that one pass of the polypeptide through the membrane is sufficient. The peptide fragments are collected onto a composite capture membrane that is chemically treated, for example by adding a mixture of nitrocellulose and MALDI matrix, so as to absorb peptides near the surface to facilitate desportion, thereby increasing the sensitivity of subsequent analysis by MALDI-TOF mass spectrometry. A wide variety of application are disclosed including identifying proteins separated on a gel or within a tissue sample.Type: GrantFiled: December 20, 2002Date of Patent: January 23, 2007Assignee: PerSeptive Biosystems Inc.Inventors: Timothy K. Nadler, Kenneth G. Parker, George J. Vella, Barrie G. Wagenfeld, Yulin Huang, Robert J. Lotti
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Patent number: 7005294Abstract: The invention suggests a method of producing an array for the detection of components from a biological sample, wherein the detection molecules are immobilized on one or more supports, said support(s) is/are embedded and subjected to curing, the support is separated into sections, and the sections are applied on another support.Type: GrantFiled: February 27, 2002Date of Patent: February 28, 2006Assignee: Attomol Moleulare Diagnostika GmbHInventor: Werner Lehmann
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Patent number: 6833060Abstract: The present invention relates generally to the electrophoretic separation of biomolecules, including but not limited to proteins, peptides, DNA and RNA. The methods of this invention are particularly useful when applied to two-dimensional gel electrophoresis.Type: GrantFiled: May 7, 2001Date of Patent: December 21, 2004Assignee: University of Louisville Research Foundation, Inc.Inventors: George Harding, Elias Klein, Jon Klein
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Patent number: 6632339Abstract: Polypeptides which have been separated by gel electrophoresis can be identified by electroblotting them through a “sandwich” comprising in order: a) the separation gel; b) at least one hydrophilic membrane, e.g. of carboxyl-modified PVDF, on which is immobilized at least one reagent capable of cleaving a polypeptide, e.g. trypsin; c) a hydrophobic layer, typically a membrane, e.g. of PVDF. Preferably a biased alternating current or discontinuous direct current is used for electroblotting. The resulting fragments, usually peptides, are identified, preferably by MALDI-TOF MS.Type: GrantFiled: June 15, 2000Date of Patent: October 14, 2003Assignee: University of GenevaInventors: Willy Vincent Bienvenut, Denis Francois Hochstrasser
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Patent number: 6602391Abstract: An apparatus and method are described for capillary separation of macromolecules and precise post-separation blotting. Apparatus include disposable separating element (capillary), which contains a sieving or interaction matrix inside, an external layer of blotting material, positioned close to the boundary of said sieving or interaction matrix, and the membrane with changeable permeability for separated material; said membrane separates blotting layer from the sieving or interaction matrix. After separation of macromolecules in capillary with initially non-permeable walls, chemical or physical modification of the membrane is performed, which is followed by changing the vector of driving forces for transfer, so that separated molecules are moved through the walls of the capillary and blotted to the outer layer of separating element, which contains blotting material. Means of modification of the membrane include chemical or physical modification, leading to changes in permeability.Type: GrantFiled: March 5, 2001Date of Patent: August 5, 2003Inventor: Vladimir B. Serikov