Blotting Patents (Class 204/464)
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Publication number: 20030032201Abstract: This application relates to a method and apparatus for the electrophoresis, blotting and detection of nucleic acids, proteins and other biological molecules using a porous polymer gel carrier and assembly. The combination of a porous polymer gel carrier and attached laminate layer facilitates the electrophoresis and subsequent blotting analysis of nucleic acids, proteins and other biological molecules. The porous polymer gel carrier also facilitates the use, handling and processing of fragile separation materials such as polyacrylimide and agarose gels. The method and apparatus also permits the convenient packaging and distribution of standardized electrophoresis and blotting products.Type: ApplicationFiled: August 10, 2001Publication date: February 13, 2003Inventor: Robert W. Flesher
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Publication number: 20020172955Abstract: Methods employing purification devices comprising an electrophoretic medium containing immobilized capture probes for the purification of DNA are disclosed.Type: ApplicationFiled: August 24, 2001Publication date: November 21, 2002Applicant: EXACT Sciences CorporationInventors: Christopher P. Adams, T. Christian Boles, Lawrence Weir, Rahul K. Dhanda, Nevin M. Summers
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Publication number: 20020166769Abstract: An apparatus and method are described for capillary separation of macromolecules and precise post-separation blotting. Apparatus include disposable separating element (capillary), which contains a sieving or interaction matrix inside, an external layer of blotting material, positioned close to the boundary of said sieving or interaction matrix, and the membrane with changeable permeability for separated material; said membrane separates blotting layer from the sieving or interaction matrix. After separation of macromolecules in capillary with initially non-permeable walls, chemical or physical modification of the membrane is performed, which is followed by changing the vector of driving forces for transfer, so that separated molecules are moved through the walls of the capillary and blotted to the outer layer of separating element, which contains blotting material. Means of modification of the membrane include chemical or physical modification, leading to changes in permeability.Type: ApplicationFiled: March 5, 2001Publication date: November 14, 2002Inventor: Vladimir B. Serikov
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Publication number: 20020157953Abstract: The present invention discloses a semi-dry electroblotter for transferring a gel after its electrophoresis onto a membrane enabling the electro-blotting process to be efficiently and effectively proceeded within a short time, and eliminating the shortcomings of the traditional technology. The electroblotter of the present invention comprises an upper block, and a corresponding lower block in the shape of a groove. A corresponding positioning member is disposed on each of the lateral sides of the upper block and the lower block, such that the upper block and the lower block will be coupled automatically when they are closed in a correct guiding position. An elastic member and an electrode plate are separately disposed in the interior of the upper block and the lower block, and the elastic member supports the electrode plate so that the electrode plate has an elastic reaction on the opposite direction when the electrode plate is pressed.Type: ApplicationFiled: April 27, 2001Publication date: October 31, 2002Inventor: Hui-Wan Chen
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Patent number: 5916429Abstract: In a horizontal gel, direct blot electrophoresis separation apparatus, device to remove gas dissolved in the buffer solution surrounding the anode at a location remote from the interface between the gel and the moving membrane, such as a gas evolution member disposed between the anode and the moving membrane of such apparatus, has been found to result in more consistent and readable blot patterns with less defects.Type: GrantFiled: August 1, 1997Date of Patent: June 29, 1999Assignee: Qualicon Inc.Inventor: Donald Harvey Brunk
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Patent number: 5882495Abstract: The present invention provides electrophoresis systems and methods for forming at least one gel therein and for conducting electrophoresis on the gel without removing the gel from the electrophoresis system. The systems of the present invention include an electrophoresis platform which is a surface onto which one or more electrophoresis gels can be cast, and at least one gel casting/electrophoresis member for use in casting at least one gel on the electrophoresis platform and conducting electrophoresis on the gel. The present invention also includes optional members specific to other stages of the electrophoresis process, including a gel staining member and a gel drying member.Type: GrantFiled: November 12, 1996Date of Patent: March 16, 1999Assignee: Proteome, Inc.Inventor: James I. Garrels
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Patent number: 5846395Abstract: An electrophoresis apparatus for automatically performing medical assays includes an electrophoresis platform which cooperates with a gantry assembly. The electrophoresis platform and the gantry assembly are movable along paths that are perpendicular to each other. An applicator assembly includes pipettes which transfer fluid samples from a specimen tray to an electrophoresis plate mounted on the electrophoresis platform. The electrophoresis platform then moves to a position into the gantry assembly, where electrophoresis is conducted to separate the samples into different fractions. The electrophoresis platform then moves beneath a reagent pouring station where a reagent is applied to make the separated fractions fluoresce under ultraviolet light. The electrophoresis platform is then moved beneath the gantry assembly again, and an air knife in the gantry assembly spreads the reagent.Type: GrantFiled: April 25, 1997Date of Patent: December 8, 1998Assignee: Helena Laboratories CorporationInventors: Robert J. Sarrine, Henry A. Garsee, Charles D. Kelley, Michael T. Everitt, Earl W. Boone, Philip A. Guadagno, Eric H. Petersen, Tipton L. Golias
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Patent number: 5840169Abstract: A process for electroelution of a gel containing charged macromolecules, such as proteins or polynucleotides, comprising the steps of providing a plurality of adjacent parallel chambers having a trapezoidal cross-section, placing a gel containing the charged macromolecules onto first open sides of the chambers, placing a semipermeable membrane onto second open sides of the chambers, filling the chambers with an elution buffer and applying a voltage difference across the chambers so that charged macromolecules in the gel migrate into the elution buffers in the chambers. Also, an apparatus for electroelution of a gel containing charged macromolecules having, preferably, a plurality of adjacent parallel chambers having a trapezoidal cross-section and vents for removing the product without disassembling the apparatus.Type: GrantFiled: March 13, 1995Date of Patent: November 24, 1998Inventor: Peter Andersen
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Patent number: 5595636Abstract: The invention relates to the mass spectrometric analysis of separated substance samples on so-called 2-D-gel electrophoresis plates, used particularly for protein determination, with ionization of the substance samples by MALDI. The molecules of the substance samples are transferred to a thin, lacquer-like smooth matrix layer which should preferably be applied to a metal sample support. Transfer takes place directly or indirectly, for example via a polyvinylidene difluoride membrane an an intermediate carrier, by electrophoretic transport of the molecules to the matrix surface. Before transfer, the proteins may be subjected to enzymatic cutting of their amino acid chains.Type: GrantFiled: March 9, 1995Date of Patent: January 21, 1997Assignee: Bruker-Franzen Analytik GmbHInventor: Jochen Franzen
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Patent number: 5593561Abstract: A multiple electrophoresis method and apparatus for migrating and transferring macromolecules in a vessel (10) containing a plurality of parallel elongate electrodes (14), membranes (22) disposed vertically in the vessel between the columns of electrodes (14), and an electrophoresis gel which is inserted in the vessel in liquid form and which is subsequently solidified to perform electrophoresis. When the electrophoresis is completed, the gel is liquefied, dissolved, or decomposed, and the membranes (22) onto which the macromolecules have been transferred are withdrawn from the vessel.Type: GrantFiled: July 18, 1995Date of Patent: January 14, 1997Assignee: Bertin & CieInventors: Dominique Cognard, Jean Hache
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Patent number: 5514256Abstract: This invention is a means for the rapid sequencing of DNA samples. More specifically, it consists of a new design direct blotting electrophoresis unit. The DNA sequence is deposited on a membrane attached to a rotating drum. Initial data compaction is facilitated by the use of a machined multi-channeled plate called a ribbon channel plate. Each channel is an isolated mini gel system much like a gel filled capillary. The system as a whole, however, is in a slab gel like format with the advantages of uniformity and easy reusability. The system can be used in different embodiments. The drum system is unique in that after deposition the drum rotates the deposited DNA into a large non-buffer open space where processing and detection can occur. The drum can also be removed in toto to special workstations for downstream processing, multiplexing and detection.Type: GrantFiled: October 28, 1994Date of Patent: May 7, 1996Assignee: Battelle Memorial InstituteInventors: Richard J. Douthart, Shannon L. Crowell