With Injector Patents (Class 204/604)
  • Patent number: 9772322
    Abstract: A cell observation system 1, for measuring fluorescence emitted from a cell held by a microplate 20 having a plurality of wells 21, comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, a position controller 30 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21, a moving image acquisition unit 40 for detecting the fluorescence from the sample S within the wells 21, and a data analyzer 50 for setting a part of a region facing the positive electrode 17b on the well 21 as an analysis region and analyzing an optical intensity in the analysis region so as to acquire analysis information concerning the sample S.
    Type: Grant
    Filed: October 23, 2013
    Date of Patent: September 26, 2017
    Assignee: HAMAMATSU PHOTONICS K.K.
    Inventors: Takuji Kataoka, Taira Ito, Natsumi Saito
  • Patent number: 9539573
    Abstract: In a method of performing dilution of a droplet in an EWOD device, a parent droplet is provided on an electrode array of the EWOD device, wherein the parent droplet has a first concentration of a species. A diluent droplet also is provided on the electrode array of the EWOD device. The method includes controlling actuation voltages applied to the electrode array of the EWOD device to join the parent droplet and the diluent droplet into a product droplet having a diluted second concentration of the species different from the first concentration in the parent droplet. The actuation voltages then are controlled to split the product droplet into one or more daughter droplets having the second concentration of the species. A dilution ratio may be calibrated based on the volumes of the droplets. Serial dilution steps may be performed to generate daughter droplets of different species concentrations at each step.
    Type: Grant
    Filed: June 23, 2015
    Date of Patent: January 10, 2017
    Assignee: Sharp Kabushiki Kaisha
    Inventors: Benjamin James Hadwen, Christopher James Brown, Julie Karen Deacon
  • Patent number: 9034161
    Abstract: The present invention provides methods, devices, and kits for separating and selecting top sperm from a sperm sample of a subject. In one aspect, for example, such a method can include removing a portion of negatively charged protein from sperm in the sperm sample, immobilizing the sperm, electrophoretically separating the sperm, and selecting mature sperm based on electromotility properties.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: May 19, 2015
    Inventors: Douglas T. Carrell, Luke Simon
  • Publication number: 20150107999
    Abstract: An apparatus for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The electric field is applied to at least one electrode having a plurality of concentric rings or concentric arcs extending radially outwards from a center point, electrically connected to a voltage source such that when voltage is applied to the at least one electrode, the concentric rings or concentric arcs alternate in voltage potential.
    Type: Application
    Filed: December 24, 2014
    Publication date: April 23, 2015
    Inventors: Monika Weber, Mark A. Reed
  • Publication number: 20150060276
    Abstract: There is provided a nanopore system including a nanopore in a solid state membrane. A first reservoir is in fluidic connection with the nanopore, the first reservoir being configured to provide, to the nanopore, nucleic acid molecules in an electrolytic solution. A second reservoir is in fluidic connection with the nanopore, with the nanopore membrane separating the first and second reservoirs. A pressure source is connected to the first reservoir to apply an external pressure to the first reservoir to cause nanopore translocation of nucleic acid molecules in the solution in the first reservoir. A voltage source is connected between the second and first reservoirs, across the nanopore, with a voltage bias polarity that applies an electric field counter to the externally applied pressure. Force of the externally applied pressure is greater than force of the electric field during nanopore translocation by the nucleic acid molecules.
    Type: Application
    Filed: September 12, 2014
    Publication date: March 5, 2015
    Applicants: PEKING UNIVERSITY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Jene A. Golovchenko, Bo Lu, David P. Hoogerheide, Dapeng Yu, Qing Zhao
  • Publication number: 20150060277
    Abstract: There is provided a nanopore system including a nanopore in a support structure. A first reservoir is in fluidic connection with the nanopore and a second reservoir is in fluidic connection with the nanopore. The support structure separates the first and second reservoirs. A pressure source is connected to one of the first and second reservoirs to apply an external pressure to one of the first and second reservoirs. A voltage source is connected between the first and second reservoirs to apply a voltage bias between the first and second reservoirs, across the nanopore. This system enables a method for analysis of species in solution, wherein there is provided to the nanopore a fluidic solution that includes a species for translocation through the nanopore, with an external pressure applied to the species in fluidic solution and a voltage bias applied across the nanopore.
    Type: Application
    Filed: September 12, 2014
    Publication date: March 5, 2015
    Applicants: PEKING UNIVERSITY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Jene A. Golovchenko, Bo Lu, David P. Hoogerheide, Dapeng Yu, Qing Zhao
  • Publication number: 20150047979
    Abstract: A method of fluid testing includes pressurizing a fluid testing system, disposed at a subterranean location under high pressure compared to a surface pressure, to achieve a desired pressure differential between the high pressure and an internal pressure of the fluid testing system. The fluid testing system includes a capillary electrophoresis system and one or more test fluid reservoirs. The method also includes directing test fluid from the one or more test fluid reservoirs into capillaries to condition the capillaries. The method further includes directing sample fluid into the capillaries for testing while at the subterranean location.
    Type: Application
    Filed: August 15, 2013
    Publication date: February 19, 2015
    Applicant: Schlumberger Technology Corporation
    Inventors: Sharath Chandra Mahavadi, Geza Horvath Szabo, Simon Ivar Andersen
  • Patent number: 8883513
    Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: November 11, 2014
    Assignee: Advanced Liquid Logic, Inc.
    Inventors: Michael G. Pollack, Vamsee Pamula, Vijay Srinivasan
  • Publication number: 20140305801
    Abstract: Aspects of the innovations presented herein relate to improved systems that in some embodiments perform capillary electrophoresis (CE) and CE in conjunction with electrospray ionization (ESI) as an input to a mass spectrometry system (MS). Some embodiments use a high voltage isolated CE power supply that is configured to float on the high voltage output of an ESI-MS power supply, with a protective resistance in the ESI-MS path, as well as DC/DC converter isolation and communication system isolation for the isolated CE power supply. Some embodiments additionally use a cartridge assembly integrating separation and conductive fluid capillaries with fluid cooling and protective retractable housings for the capillary end portions and for the ESI output. The protective housing may further be used with an adapter for interfacing with different MS systems.
    Type: Application
    Filed: October 26, 2012
    Publication date: October 16, 2014
    Inventors: Brian D. Peterson, Dale Alan Owens, Peter S. Kotowski, Sunil S. Deliwala, Craig A. Hudson, James H. Reyes, Clive Cheung, Tom Tran, Anna X. Lou, Jian (Jane) Ying Lou, Jean-Marc Rene Lucien Busnel, Chitra K. Ratnayake
  • Publication number: 20140291155
    Abstract: Microfluidic sample injection, which is based on a mechanical valve rather than electrokinetic injection into an integrated separation channel or a discrete separation column, can provide improved sample injections, enhanced capabilities, and can eliminate the need for changing the electric field in the separation channel to induce sample injection. An interface allowing the use of a discrete separation column easily allows for flexibility to utilize the microfluidic injector with existing analytical techniques. Multiple sample channels and/or sample sources can be utilized with the microfluidic sample injector.
    Type: Application
    Filed: April 29, 2014
    Publication date: October 2, 2014
    Applicant: BATTELLE MEMORIAL INSTITUTE
    Inventors: Ryan T. Kelly, Keqi Tang
  • Patent number: 8841116
    Abstract: Methods and microfluidic circuitry for inline injection of nucleic acids for capillary electrophoresis analysis are provided. According to various embodiments, microfabricated structures including affinity-based capture matrixes inline with separation channels are provided. The affinity-based capture matrixes provide inline sample plug formation and injection into a capillary electrophoresis channel. Also provided are methods and apparatuses for a microbead-based inline injection system for DNA sequencing.
    Type: Grant
    Filed: October 25, 2007
    Date of Patent: September 23, 2014
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, Robert Blazej, Palani Kumaresan
  • Patent number: 8834697
    Abstract: A channel (12) is formed inside a transparent substrate (11), and the two ends are respectively connected to a sample inlet (13) and a separation medium filling port (14) both open to outside, and a sample outlet (15) which is open to outside is provided on the channel (12). The portion between the sample inlet (13) and the sample outlet (15) is a separation channel portion (12a), and the portion between the sample outlet (15) and the separation medium filling port (14) is a separation medium introduction channel portion (12b). A separation medium supplier is connected to the separation medium filling port (14) and the separation medium introduction channel portion (12b) is filled with a separation medium. Then a sample is dropped to the sample inlet (13), with the sample medium supplier still connected, to introduce the sample to the separation channel portion (12a). And then, buffer liquid is poured in buffer reservoirs (16) and (17) and a migration voltage is applied to perform a migration analysis.
    Type: Grant
    Filed: April 25, 2007
    Date of Patent: September 16, 2014
    Assignee: Shimadzu Corporation
    Inventor: Toru Kaji
  • Patent number: 8828209
    Abstract: This invention discloses a highly efficient method, system and apparatus for nucleic acid analysis, including sequencing (both automated re-sequencing and de-novo sequencing). The system is capable of sequencing DNA sizes ranging from fragments to mammalian size genomes having mouse draft quality at a much reduced cost. The system comprises a massive parallel capillary electrophoretic separation using two-dimensional monolith multi-capillary arrays (2D-MMCA). Sequence identification can be performed using fluorescent or otherwise labeled dideoxynucleotide-terminated DNA extension product generated by gel matrix-, or beads-, or substrate tethered-, or otherwise immobilized colonies of single template molecules.
    Type: Grant
    Filed: June 22, 2006
    Date of Patent: September 9, 2014
    Assignee: The Research Foundation for the State University of New York
    Inventors: Vera Gorfinkel, Evgeni A. Kabotyanski, Boris Gorbovitski
  • Publication number: 20140238856
    Abstract: Devices for controlling the capture, trapping, and transport of macromolecules include at least one fluidic transport nanochannel that intersects and is in fluid communication with at least one transverse nanochannel with (shallow) regions and/or with integrated transverse electrodes that enable fine control of molecule transport dynamics and facilitates analyses of interest, e.g., molecular identification, length determination, localized (probe) mapping and the like.
    Type: Application
    Filed: February 26, 2014
    Publication date: August 28, 2014
    Applicant: The University of North Carolina at Chapel Hill
    Inventors: John Michael Ramsey, Laurent Menard
  • Patent number: 8708965
    Abstract: A scalable parallel injector array has a support structure for holding injector tubes in a spatial pattern in order to enable delivery of fluidic materials simultaneously to a group of specific target areas in the brain or a body part. Each injector is individually connectible at one end to a displacement-controlled pump and is designed at the other end to be inserted into the brain or body part. The injector tubes are connected to the pump by fluid-filled tubing. The spatial pattern is patient- and/or application-customizable, and the length of the injector tubes is customizable in order to more precisely reach individual target areas. A clamping device may be used to attach the injector array to a stereotaxic arm or other support structure. The action of the pump permits controlled simultaneous delivery of the fluidic materials to the target areas.
    Type: Grant
    Filed: November 17, 2009
    Date of Patent: April 29, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Edward S. Boyden, Stephanie Chan
  • Patent number: 8685220
    Abstract: The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.
    Type: Grant
    Filed: July 26, 2012
    Date of Patent: April 1, 2014
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Takeshi Ohura, Ryoji Inaba, Yoshitaka Kodama, Hiromi Yamashita, Manabu Akiba, Tomohiro Shoji
  • Publication number: 20140021050
    Abstract: Described are methods of reducing the incidence and/or magnitude of artifacts in denaturing nucleic acid capillary electrophoresis (CE). Methods and systems described serve to dismiss non-denatured DNA from the tip of the capillary after sample injection and prior to electrophoretic separation of loaded nucleic acids. Among the methods disclosed are the application of a brief reverse-polarity pulse to the capillary prior to separation but after removal of the capillary from the sample reservoir, and transiently heating the capillary to cause expansion of the separation matrix after removal of the capillary from the sample reservoir.
    Type: Application
    Filed: January 30, 2012
    Publication date: January 23, 2014
    Applicant: PRIMERADX, INC.
    Inventors: Sergey V. Voronov, Jork Nolling
  • Publication number: 20130341189
    Abstract: A method for nucleic acid analysis including injecting a slight amount of nucleic acid sample for analysis, characterized in that most of a nucleic acid sample which is not used, excluding the slight amount of the nucleic acid sample which is used for analysis, can be obtained as a pure product which is not contaminated with a fluorescent material, and a microchip for analyzing nucleic acid which enables such method for nucleic acid analysis, are provided. The method for nucleic acid analysis may analyze at least two different nucleic acid samples in a continuous manner by sequentially injecting the at least two different nucleic acid samples.
    Type: Application
    Filed: March 7, 2013
    Publication date: December 26, 2013
    Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION
    Inventors: JONG HOON HAHN, BYOUNG JOO KWAK, HAN-OK KIM
  • Publication number: 20130313116
    Abstract: A microfluidic chip interface for providing fluid communication with external fluid sources and external fluid waste containers, and for providing electrical contact with voltage sources and voltage and current measuring devices, is described. The microchip is first placed into electrical communication with at least one electrical source and at least one electronic measurement device, and reversibly secured in place. Chosen fluids are provided into the microchip and directed through the chip using a fluid manifold having dispensing tubes and fluid aspiration tubes, which is brought into the vicinity of the secured microchip. The distance between the fluid manifold and the microchip is chosen such that the injection tubes are located within wells in the microchip connected to microfluidic channels, and the aspiration tubes are located near the surface of the microchip in the vicinity of the wells such that fluid spillage onto the surface of the microchip during fluid transfer is avoided.
    Type: Application
    Filed: November 14, 2012
    Publication date: November 28, 2013
    Inventors: James T. Palmer, Philippe M. Dekleva
  • Publication number: 20130248369
    Abstract: A capillary electrophoresis system which provides a microfluidic chip for capillary electrophoresis and a microfluidic interface module which fluidicly couples the microfluidic chip to external fluid sources and or external repositories.
    Type: Application
    Filed: February 25, 2011
    Publication date: September 26, 2013
    Applicant: Advanced Micro Labs ,LLC
    Inventors: Philippe M. Dekleva, Jonathan A. Vickers, James T. Palmer
  • Publication number: 20130161193
    Abstract: A microfluidic system includes a microfluidic device; and a metered fluid loading system formed integrally with the microfluidic device and configured to load a discrete metered volume of fluid into the microfluidic device upon actuation.
    Type: Application
    Filed: December 21, 2011
    Publication date: June 27, 2013
    Applicant: SHARP KABUSHIKI KAISHA
    Inventors: Adrian Marc Simon JACOBS, Campbell Donald BROWN, Benjamin James HADWEN, Jason Roderick HECTOR
  • Patent number: 8470149
    Abstract: A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.
    Type: Grant
    Filed: December 5, 2011
    Date of Patent: June 25, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Charles S. Vann, Debjyoti Banerjee, Timothy G. Geiser, James C. Nurse, Nigel P. Beard
  • Publication number: 20130153424
    Abstract: Provided is a microchip, including independently an introduction area inside having a pressure negative to atmospheric pressure and into which a liquid is injected by puncturing, and a degassing area inside having a pressure negative to atmospheric pressure for degassing a cavity of a hollow tube that punctures the introduction area for injecting the liquid.
    Type: Application
    Filed: December 10, 2012
    Publication date: June 20, 2013
    Applicant: SONY CORPORATION
    Inventor: Sony Corporation
  • Patent number: 8460531
    Abstract: An integrated bio-analysis system incorporates built-in sample preparation capabilities. In one aspect of the present invention, a bio-analysis instrument is provided with a built-in sample preparation device based on PCR (or thermal cycling block/module). In one embodiment of the present invention, a peltier unit in the sample preparation device provides thermal cycling of samples supported in a multi-well tray. In another aspect of the present invention, a CE instrument is provided with a built-in sample preparation capability, which may comprise a sample preparation (bio-molecular reaction) device based on thermal cycler type. In another aspect of the present invention, a PCR device is provided with a built-in analysis device, such as a CE device, for verifying the results of the PCR (bio-molecular reaction) process.
    Type: Grant
    Filed: December 6, 2010
    Date of Patent: June 11, 2013
    Assignee: Qiagen Sciences, LLC
    Inventors: Varouj Amirkhanian, Ming-Sun Liu
  • Patent number: 8377277
    Abstract: Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis comprises a loading channel, a separation channel, and an injection channel. The loading channel is in fluid communication with a first and second sample port. The separation channel is connected to the loading channel to form a first intersection, and an injection channel connected to the separation channel to form a second intersection and in fluid communication with a first reservoir, and wherein the separation channel is in fluid communication with a second reservoir. The electrophoresis system further comprises two electrodes coupled to the first sample port and the first reservoir, and the first sample port and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the first reservoir and form a sample plug in the separation channel, and to further move the sample plug into the separation channel towards the second reservoir.
    Type: Grant
    Filed: October 22, 2008
    Date of Patent: February 19, 2013
    Assignee: General Electric Company
    Inventors: Jun Xie, Wei-Cheng Tian, Shashi Thutupalli, Li Zhu, Anthony John Murray, Erin Jean Finehout
  • Patent number: 8366897
    Abstract: A microfluidic apparatus and method for performing electrophoretic separation of compounds. The method comprises the steps of: a) providing a separation buffer; b) providing a sample solution in fluid contact with the separation buffer; c) applying an electric field to the separation buffer; and d) producing a variable bulk flow of the separation buffer in a direction substantially aligned with said electric field. Fluid contact between the separation buffer and the sample solution is made through a separation column having a length in the range of from approximately 0.01 mm to approximately 5 mm. By the foregoing, compounds can be sequentially detected and quantified.
    Type: Grant
    Filed: October 12, 2009
    Date of Patent: February 5, 2013
    Assignee: National Institute of Standards and Technology
    Inventors: David J. Ross, Sam Forry, Jason Kralj
  • Patent number: 8349161
    Abstract: Bubbles can be removed regardless of an individual difference of a pump to fill an electrophoresis medium into a capillary. Of flow passages formed between an inner side surface of a container for accommodating the electrophoresis medium and a side surface of a plunger, one of the flow passages causing an electrophoresis medium to be easily stagnant is formed to have the cross-sectional area larger than the cross-sectional area of the other flow passage on the opposite side. In other words, the flow passage portion causing the electrophoresis medium to be easily stagnant is formed in such a manner as to increase a flow amount of the electrophoresis medium. This can eliminate a region having an extremely small amount of electrophoresis medium flow in the pump.
    Type: Grant
    Filed: April 6, 2010
    Date of Patent: January 8, 2013
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Takashi Gomi, Ryoji Inaba, Taro Nakazawa, Takeshi Ohura, Mari Kotoura
  • Publication number: 20120318672
    Abstract: Microchip capillary electrophoresis (CE) utilizing a sample injector based on a mechanical valve rather than electrokinetic injection can provide improved sample injections, enhanced capabilities, and can eliminate the need for changing the electric field in the separation channel to induce sample injection. In one instance CE electrodes continuously apply an electric field for CE separation along a separation channel. A sample channel is connected to the separation channel at an intersection and has a sample pressure that is greater than that which is present in the separation channel near the intersection. The sample channel does not have electrodes that apply voltages for electrokinetic injection. A sample injector in the sample channel or at the intersection comprises a mechanical valve to control sample injection from the sample channel to the separation channel.
    Type: Application
    Filed: August 28, 2012
    Publication date: December 20, 2012
    Applicant: BATTELLE MEMORIAL INSTITUTE
    Inventors: Xuefei Sun, Ryan T. Kelly, Keqi Tang, Richard D. Smith
  • Publication number: 20120298511
    Abstract: A channel device including a nanosize channel through which single molecule flows, at least one electrode pair arranged near the nanosize channel, and an AC power source that applies an AC voltage to the electrodes. This channel device is useful for identifying molecules one by one. Furthermore, a channel device including a nanosize channel through which single molecule flows, a branching portion, and a plurality of branching channels, wherein (i) an electrode pair is arranged near the nanosize channel so as to sandwich the nanosize channel between the electrodes, or (ii) one electrode of the electrode pair is located near the nanosize channel, whereas the other is arranged near the branching channels. This channel device is useful for separating single molecule. The present channel device achieves identification or separation at an accuracy of 100% in principle. A sample treatment apparatus according to present invention includes a channel device, a measurement section, and an arithmetic processing section.
    Type: Application
    Filed: July 26, 2010
    Publication date: November 29, 2012
    Inventor: Takatoki Yamamoto
  • Patent number: 8298393
    Abstract: A device for electrophoretic analysis of multicomponent solutions comprises a capillary and vials for electrolyte and sample solutions. The device comprises streaming potential measurement means implemented to measure a potential difference between capillary ends and to form an electric connections with said capillary ends during capillary rinsing in such a way that said streaming potential measurement means and electrolyte inside capillary and vials form a closed measurement electric circuit. A means for generation of electrolyte flow is implemented so as to build up and maintain a preset differential pressure between capillary ends and wherein that streaming potential measurement means comprises means to disconnect said measurement electric circuit during electrophoretic analysis.
    Type: Grant
    Filed: February 5, 2007
    Date of Patent: October 30, 2012
    Assignee: Lumex Instruments Limited
    Inventors: Mikhail Ivanovich Zinchenko, Yaroslav Sergeevich Kamentsev, Mikhail Yaroslavovich Kamentsev, Slyadnev Nikolaevich Maksim, Alexander Anatolevich Stroganov
  • Publication number: 20120261013
    Abstract: A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.
    Type: Application
    Filed: June 19, 2012
    Publication date: October 18, 2012
    Applicant: CYTONOME/ST, LLC
    Inventors: John Richard Gilbert, Sebastian Böhm, Manish Deshpande
  • Publication number: 20120255861
    Abstract: This technology is a method and apparatus for the semi-continuous measurement of the concentration of constituents of airborne particles which couples a laminar flow, water condensation particle collector to a microfluidic device for assay of particle chemical composition by electrophoresis. The technology has been used for the assay of sulfates, nitrates, chlorides, and organic acids contained in fine and submicrometer atmospheric particles. For these compounds the apparatus and method described is capable of one-minute time resolution at concentrations at the level of micrograms of analyte species per cubic meter of air. Extension to other analytes is possible.
    Type: Application
    Filed: September 27, 2011
    Publication date: October 11, 2012
    Inventors: Susanne Vera Hering, Gregory Stephen Lewis, Steven Russel Spielman, Charles Sherman Henry, Scott Douglas Noblitt, Jeffrey Lee Collett, JR.
  • Patent number: 8277659
    Abstract: Microchip capillary electrophoresis (CE) utilizing a sample injector based on a mechanical valve rather than electrokinetic injection can provide improved sample injections, enhanced capabilities, and can eliminate the need for changing the electric field in the separation channel to induce sample injection. In one instance CE electrodes continuously apply an electric field for CE separation along a separation channel. A sample channel is connected to the separation channel at an intersection and has a sample pressure that is greater than that which is present in the separation channel near the intersection. The sample channel does not have electrodes that apply voltages for electrokinetic injection. A sample injector in the sample channel or at the intersection comprises a mechanical valve to control sample injection from the sample channel to the separation channel.
    Type: Grant
    Filed: September 23, 2010
    Date of Patent: October 2, 2012
    Assignee: Battelle Memorial Institute
    Inventors: Xuefei Sun, Ryan T. Kelly, Keqi Tang, Richard D. Smith
  • Patent number: 8257570
    Abstract: In order to provide a high-performance electrophoresis chip and an electrophoresis unit having the same that can restrain the diffusion of sample at an intersection between the electrophoresis groove and the sample introduction groove and prevent decrease in contrast and decrease in resolution, an electrophoresis chip is provided with a sample introduction groove, an electrophoresis groove, and a through hole. The sample introduction groove, the electrophoresis groove, and the through hole are formed on different substrates. In the electrophoresis chip, by combining the substrates, the sample introduction groove and the electrophoresis groove are located in different planes.
    Type: Grant
    Filed: January 12, 2010
    Date of Patent: September 4, 2012
    Assignee: ARKRAY, Inc.
    Inventors: Takayuki Taguchi, Shigeru Kitamura, Hiroshi Fukuya
  • Patent number: 8252162
    Abstract: The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.
    Type: Grant
    Filed: December 24, 2008
    Date of Patent: August 28, 2012
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Takeshi Ohura, Ryoji Inaba, Yoshitaka Kodama, Hiromi Yamashita, Manabu Akiba, Tomohiro Shoji
  • Patent number: 8241476
    Abstract: A novel on-line method is presented for the extraction and preconcentration of amino acids using a sol-gel coated column coupled to a conventional UV/vis detector. Extraction, stacking and focusing techniques are used in the preconcentration procedures. Sol-gel coatings are created by using N-Octadecyldimethyl[3(trimethoxysilyl)proply]ammonium chloride (C18-TMS) in the coating sol solutions. The resulting sol-gel coating carries a positive charge. For extraction, the pH of the samples is properly adjusted to impart a net negative charge to amino acids. A long plug of the sample is then passed through the sol-gel coated capillary to facilitate extraction via electrostatic interaction between the positively charged sol-gel coating and the negatively charged amino acid molecules. The focusing of the extracted amino acids is accomplished through desorption of the extracted amino acid molecules carried out by local pH change. The described procedure provides 150,000-fold enrichment effect for alanine.
    Type: Grant
    Filed: May 14, 2008
    Date of Patent: August 14, 2012
    Assignee: University of South Florida
    Inventors: Abdul Malik, Wen Li, David P. Fries
  • Patent number: 8221606
    Abstract: In order to provide a high-performance electrophoresis chip and an electrophoresis unit having the same that can restrain the diffusion of sample at an intersection between the electrophoresis groove and the sample introduction groove and prevent decrease in contrast and decrease in resolution, an electrophoresis chip is provided with a sample introduction groove, an electrophoresis groove, and a through hole. The sample introduction groove, the electrophoresis groove, and the through hole are formed on different substrates. In the electrophoresis chip, by combining the substrates, the sample introduction groove and the electrophoresis groove are located in different planes.
    Type: Grant
    Filed: January 12, 2010
    Date of Patent: July 17, 2012
    Assignee: ARKRAY, Inc.
    Inventors: Takayuki Taguchi, Shigeru Kitamura, Hiroshi Fukuya
  • Patent number: 8187442
    Abstract: The processing apparatus for microchips, each at least having a main flow path performing migration of a sample for analysis inside a sheet-like member, comprises a holding part holding microchips so that the multiple main flow paths are provided; a pretreatment part common to the multiple main flow paths for performing a pretreatment step prior to an analysis step in each of the multiple main flow paths; a processing part for performing analysis in each of the main flow paths independently of the others; and a control part controlling an operation in the pretreatment part so that when a pretreatment step for one main flow path ends, a pretreatment for a next main flow path starts and further controlling an operation in the processing part so that an analysis is subsequently performed for the main flow path where the pretreatment step has ended.
    Type: Grant
    Filed: December 31, 2008
    Date of Patent: May 29, 2012
    Assignee: Shimadzu Corporation
    Inventors: Nobuhiro Hanafusa, Akihiro Arai, Koji Tanimizu
  • Patent number: 8182746
    Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.
    Type: Grant
    Filed: October 31, 2007
    Date of Patent: May 22, 2012
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Patent number: 8177951
    Abstract: To obtain a stable pressure to inject polymer into a capillary array in order to shorten the injection time and raise the processing capacity of the apparatus. Disclosed herein is an electrophoresis apparatus comprising one or more capillaries to be filled with an electrophoretic medium and a pump mechanism which can be connected to the capillaries for filling the capillaries with an electrophoretic medium. The pump mechanism comprises a plunger; a slider which is bonded to the plunger and moves together with the plunger; a spring which gives force to the slider; a cover which limits the range of displacement of the slider; and a linear motion system which is bonded to the cover and moves linearly, wherein the cover and the slider move as one body when the electrophoretic medium is taken to the plunger, and the plunger is pushed by the action of the spring when the electrophoretic medium is injected from the plunger into the capillaries.
    Type: Grant
    Filed: May 19, 2008
    Date of Patent: May 15, 2012
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Tomohiro Shoji, Takashi Gomi
  • Publication number: 20120090997
    Abstract: Microelectrode comprising a body formed from electrically non-conducting material and including at least one region of electrically conducting material and at least one passage extending through the body of non-conducting material and the region of conducting material, the electrically conducting region presenting an area of electrically conducting material to a fluid flowing through the passage in use. An electrochemical cell which includes such a microelectrode is also disclosed.
    Type: Application
    Filed: December 22, 2011
    Publication date: April 19, 2012
    Inventors: Andrew John WHITEHEAD, Geoffrey Alan Scarsbrook, Julie Victoria Macpherson, Mark Newton, Patrick Robert Unwin, William Joseph Yost, III
  • Publication number: 20120080316
    Abstract: A separation module operates to fractionate or separate an analyte into fractions according to pI, i.e., pI bands, utilizing capillary isoelectric focusing (“CIEF”) within a first microchannel. The fractions are stacked to form plugs, the number of which is determined by a number of parallel second microchannels integrally connected to the first microchannel, into which the fractions are directed according to the buffer characteristics found in each of the individual microchannels. Within the microchannels the plugs are separated into proteins according to a different chemical property, i.e., “m/z,” utilizing capillary electrophoresis (“CE”).
    Type: Application
    Filed: December 12, 2011
    Publication date: April 5, 2012
    Inventors: Thomas Wayne Schneider, James N. Baraniuk
  • Publication number: 20120080313
    Abstract: Electroosmotic (EO) devices are provided which are not subject to mechanical wear and tear and with no moving parts, and having improved flow rates and electrical properties. Atomic layer deposition can be used to prepare three electrical terminal active zeta potential modulated EO devices from porous membranes. First, second, and further thin layers of materials can be formed with the pores. Thus, embedded electrodes can be formed along the length of the pores. The zeta potential in the pores can be modified by use of a voltage potential applied the embedded electrode, thereby achieving active control of surface zeta potential within the pores and active control of flow through the pores.
    Type: Application
    Filed: October 24, 2011
    Publication date: April 5, 2012
    Applicant: Old Dominion University Research Foundation
    Inventors: Helmut BAUMGART, Diefeng Gu, Tarek Abdel-Fattah, Ali Beskok, Seungkyung Park
  • Publication number: 20120074066
    Abstract: Microchip capillary electrophoresis (CE) utilizing a sample injector based on a mechanical valve rather than electrokinetic injection can provide improved sample injections, enhanced capabilities, and can eliminate the need for changing the electric field in the separation channel to induce sample injection. In one instance CE electrodes continuously apply an electric field for CE separation along a separation channel. A sample channel is connected to the separation channel at an intersection and has a sample pressure that is greater than that which is present in the separation channel near the intersection. The sample channel does not have electrodes that apply voltages for electrokinetic injection. A sample injector in the sample channel or at the intersection comprises a mechanical valve to control sample injection from the sample channel to the separation channel.
    Type: Application
    Filed: September 23, 2010
    Publication date: March 29, 2012
    Applicant: BATTELLE MEMORIAL INSTITUTE
    Inventors: Xuefei Sun, Ryan T. Kelly, Keqi Tang, Richard D. Smith
  • Patent number: 8142635
    Abstract: A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.
    Type: Grant
    Filed: July 26, 2010
    Date of Patent: March 27, 2012
    Assignee: Hitachi, Ltd.
    Inventors: Yasushi Shimizu, Toshiaki Kita, Kiyoshi Tsukada, Akihiro Suzuki, Tomonari Morioka, Masaya Kojima, Ryoji Inaba, Daizo Tokinaga, Shuhei Yamamoto, Yoshiyuki Okishima, Masao Fukunaga, Takayasu Furukawa, Tomohiro Shoji
  • Publication number: 20120061240
    Abstract: A method of controlling nematode response in a microfluidic environment is provided comprising exposing the nematode to an electric field that induces a nematode response. In one embodiment, a method of sorting a group of nematodes based on a selected parameter is provided comprising the step of exposing the nematodes to an electric field that induces a differential response among the nematodes based on the selected parameter, wherein the differential response functions to separate the nematodes based on the selected parameter. Devices useful to achieve these methods are also provided.
    Type: Application
    Filed: September 15, 2011
    Publication date: March 15, 2012
    Inventors: Ravi (Ponnambalam) Selvaganapathy, Bhagwati Gupta, Pouya Rezai
  • Publication number: 20120048734
    Abstract: An analysis apparatus is provided with a storage tank, an injection nozzle, a syringe, a collection nozzle, a test sample tank, a microchip having two or more separation channels, detectors, a waste liquid tank, a controller, and a power supply. The collection nozzle collects a specimen which becomes a test sample from a test sample container housing the specimen, and transfers the specimen to the test sample tank. The separation channels separate characteristic components contained in the test sample. The injection nozzle is distanced from the collection nozzle and injects the test sample from the test sample tank into the separation channels. The detectors detect the separated characteristic components in the separation channels.
    Type: Application
    Filed: August 25, 2011
    Publication date: March 1, 2012
    Applicant: ARKRAY, Inc.
    Inventors: Koji Sugiyama, Daisuke Matsumoto, Yasunori Shiraki, Satoshi Yonehara
  • Patent number: 8123925
    Abstract: An electrophoretic apparatus that allows bubbles to be readily removed out of an electrophoretic passage. The passage for electrophoretic medium, at a connecting section where capillaries filled with electrophoretic medium are connected with a pumping mechanism for filling the electrophoretic medium, is arranged such that the side of the pumping mechanism is disposed below the side of the capillaries, so that the electrophoretic medium flows from down to up at the connecting section when filling the electrophoretic medium into the capillaries. Preferably, the passage between the capillary array and the buffer solution is controlled by using a rotary-type valve having high withstand pressure to simplify the passage structure. The dead volume of the passage can be reduced and the valuable electrophoretic medium can be efficiently used. The amount of used electrophoretic medium required for the removal of the bubble can be also reduced.
    Type: Grant
    Filed: November 21, 2007
    Date of Patent: February 28, 2012
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Tomohiro Shoji, Jin Matsumura, Hidenori Namba
  • Publication number: 20120037508
    Abstract: An electrophoresis apparatus that applies voltage from electrodes that are provided in a capillary flow channel and causes component separation by performing electrophoresis on a specimen that is injected into the capillary flow channel comprises: a physical quantity acquisition unit and a physical quantity determination unit. The physical quantity acquisition unit, with migration solution and specimen injected inside the capillary flow channel, acquires an electrical quantity that occurs in the capillary flow channel at a specified time when voltage is being applied to the electrodes. The physical quantity determination unit determines whether or not the electrical quantity that the physical acquisition unit acquires is within a specified range.
    Type: Application
    Filed: August 9, 2011
    Publication date: February 16, 2012
    Applicant: ARKRAY, Inc.
    Inventors: Yasunori Shiraki, Genki Adachi, Rina Matsumi, Toru Odagaki, Yusuke Nakayama
  • Patent number: RE43122
    Abstract: The present invention provides an on-chip packed reactor bed design that allows for an effective exchange of packing materials such as beads at a miniaturized level. The present invention extends the function of microfluidic analysis systems to new applications including on-chip solid phase extraction (SPE) and on-chip capillary electrochromatography (CEC). The design can be further extended to include integrated packed bed immuno- or enzyme reactors.
    Type: Grant
    Filed: June 22, 2010
    Date of Patent: January 24, 2012
    Assignee: The Governors of the University of Alberta
    Inventors: D. Jed Harrison, Richard Oleschuk, Loranelle L. Lockyear, Cameron Skinner, Paul Li