Involving Enzyme Or Micro-organism Patents (Class 205/777.5)
  • Patent number: 11561198
    Abstract: Device and methods for use in a biosensor comprising a multisite array of test sites, the device and methods being useful for modulating the binding interactions between a (biomolecular) probe or detection agent and an analyte of interest by modulating the pH or ionic gradient near the electrodes in such biosensor. An electrochemically active agent that is suitable for use in biological buffers for changing the pH of the biological buffers. Method for changing the pH of biological buffers using the electrochemically active agents. The methods of modulating the binding interactions provided in a biosensor, analytic methods for more accurately controlling and measuring the pH or ionic gradient near the electrodes in such biosensor, and analytic methods for more accurately measuring an analyte of interest in a biological sample.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: January 24, 2023
    Assignee: ROBERT BOSCH GMBH
    Inventors: Christopher Johnson, Sam Kavusi, Nadezda Fomina, Habib Ahmad, Autumn Maruniak, Christoph Lang, Ashwin Raghunathan
  • Patent number: 11535881
    Abstract: The present invention relates to an enzymatic electrode comprising a conductive surface and wherein a conjugate comprising at least one enzyme molecule is covalently bound to the conductive surface. The electrode is suitable for continuous analyte monitoring, particularly for continuous glucose monitoring (CGM) with glucose oxidase (GOD) as enzyme molecule. Further, the invention relates to an electrochemical sensor for measuring the concentration of an analyte, e.g. glucose under in vivo conditions comprising the enzymatic electrode.
    Type: Grant
    Filed: February 16, 2018
    Date of Patent: December 27, 2022
    Assignee: ROCHE DIABETES CARE, INC.
    Inventors: Reinhold Mischler, Angelika Fuerst, Daniel Kammerer
  • Patent number: 11512384
    Abstract: Analyte sensors and methods for fabricating analyte sensors are provided. In an exemplary embodiment, a method for fabricating a planar flexible analyte sensor includes sputtering platinum onto a polyester base layer to form a layer of platinum. The method includes patterning the layer of platinum to form working electrodes and additional electrodes. Further, the method includes forming an insulating dielectric layer over the base layer, wherein the insulating dielectric layer is formed with openings exposing portions of the working electrodes and portions of the additional electrodes. Also, the method includes partially singulating individual sensors from the base layer, wherein each individual sensor is connected to the base layer by a tab. The method further includes depositing an enzyme layer over the exposed portions of the working electrodes and coating the working electrodes with a glucose limiting membrane.
    Type: Grant
    Filed: February 20, 2018
    Date of Patent: November 29, 2022
    Assignee: Medtronic MiniMed, Inc.
    Inventors: Santhisagar Vaddiraju, Dennis Slomski
  • Patent number: 11378549
    Abstract: A gas sensor comprises a basic part and a sensing layer deposited on the basic part. The basic part includes a circuit board and at least one surface acoustic wave element disposed on the circuit board. The sensing layer is a nanocomposite film of reduced graphene oxide/tungsten oxide/polypyrrole deposited on the surface acoustic wave element. The sensing layer combines reduced graphene oxide, metal oxide, and conductive polymer, so that the sensing layer is able to perform sensing at room temperature, and can be more sensitive. The present invention provides a method for manufacturing a gas sensor, and a gas sensing system including the gas sensor.
    Type: Grant
    Filed: March 20, 2020
    Date of Patent: July 5, 2022
    Assignee: I-SHOU UNIVERSITY
    Inventors: Chi-Yen Shen, Tien-Tsan Hung, Jing-Jay Chiu, Ming Wen Yang
  • Patent number: 11360042
    Abstract: The present disclosure relates to biosensors (10) having a receptor layer (5) and a mediator layer (6), the receptor layer including ethylene receptor molecules. The present disclosure also relates to sensor units (20) comprising one or more biosensors (10) and a controller (11). In some embodiments, one or more sensor units (20) may be in wireless communication with a receiver module or a network gateway.
    Type: Grant
    Filed: June 3, 2021
    Date of Patent: June 14, 2022
    Assignee: Strella Biotechnology Company
    Inventor: Katherine Konstantin Sizov
  • Patent number: 11352657
    Abstract: Provided herein are glucose and galactose biosensors and methods of making and using the same.
    Type: Grant
    Filed: March 4, 2016
    Date of Patent: June 7, 2022
    Assignee: Duke University
    Inventors: Homme W. Hellinga, Malin Allert
  • Patent number: 11333626
    Abstract: A biological sample analysis chip including a first substrate, a membrane disposed on the first substrate, a first liquid tank which is provided with a first electrode, a plurality of second liquid tanks each of which is provided with at least one flow path and a second electrode; and a second substrate disposed below the first substrate, in which the plurality of second liquid tanks are substantially insulated from each other, the membrane disposed on the first substrate is disposed between the first liquid tank and the plurality of second liquid tanks so as to form a portion of the first liquid tank and a portion of the plurality of second liquid tanks, and the second substrate is provided with the at least one flow path and the second electrode so as to form a portion of the plurality of second liquid tanks.
    Type: Grant
    Filed: February 22, 2016
    Date of Patent: May 17, 2022
    Assignee: HITACHI, LTD.
    Inventors: Mayu Aoki, Kenichi Takeda, Kunio Harada
  • Patent number: 11278227
    Abstract: Provided is a biometric information measurement method. The method includes providing a bio-material including at least one of cells and tissues, contacting a first electrode and a second electrode with the bio-material and applying a first electrical signal and a second electrical signal to the bio-material, sensing a third electrical signal from the bio-material, and analyzing an oxygen concentration in the bio-material from the third electrical signal.
    Type: Grant
    Filed: September 10, 2019
    Date of Patent: March 22, 2022
    Assignee: Electronics and Telecommunications Research Institute
    Inventors: Young Sam Park, Jaehyun Moon, Seongdeok Ahn, Byoung Gon Yu, Jeong Ik Lee, Nam Sung Cho, Doo-Hee Cho
  • Patent number: 11268926
    Abstract: It is intended to provide, for example, a liquid sample measurement device capable of measuring the amounts of components of a liquid with a high accuracy. A first voltage is applied to an electrode pair 21, 22, which composes a biosensor 1, to obtain a first response value, a second voltage is applied to an electrode pair 23, 24, which composes the biosensor 1, to obtain a second response value, and a current that is generated when a third voltage is applied to an electrode pair 23, 27, which composes the biosensor 1, is detected to obtain a third response value. A liquid sample measurement device 6 uses the first response value, the second response value, and the third response value to obtain the concentration of glucose and the amount of blood cells of blood as well as the value equivalent to the temperature of the biosensor 1.
    Type: Grant
    Filed: April 26, 2019
    Date of Patent: March 8, 2022
    Assignee: PHC Holdings Corporation
    Inventors: Masaki Fujiwara, Tomohiro Yamamoto
  • Patent number: 11253177
    Abstract: A sensor assembly for detecting at least one analyte in a body fluid includes an electrochemical sensor, a body mount that attaches to a body of a user and an inserter that transfers the sensor to the body mount. A first adhesive is attached to one or both of the body mount or the sensor, and the first adhesive attaches the sensor to the body mount. A second adhesive is attached to one or both of the sensor or the inserter and releasably attaches the sensor to the inserter. The assembly has an initial position in which the sensor is attached to the inserter via the second adhesive and a final position in which the sensor is attached to the body mount via the first adhesive. Transferring the sensor from the initial position to the final position releases the sensor from the inserter.
    Type: Grant
    Filed: May 17, 2018
    Date of Patent: February 22, 2022
    Assignee: Roche Diabetes Care, Inc.
    Inventors: Herbert Harttig, Oliver Kube, Michael Orth, Helmut Walter
  • Patent number: 11213229
    Abstract: An apparatus for insertion of a medical device in the skin of a subject is provided.
    Type: Grant
    Filed: April 2, 2021
    Date of Patent: January 4, 2022
    Assignee: ABBOTT DIABETES CARE INC.
    Inventors: Phillip Yee, Christopher A. Thomas, Udo Hoss, Lei He, Michael R. Love
  • Patent number: 11202591
    Abstract: An apparatus for insertion of a medical device in the skin of a subject is provided.
    Type: Grant
    Filed: April 2, 2021
    Date of Patent: December 21, 2021
    Assignee: ABBOTT DIABETES CARE INC.
    Inventors: Phillip Yee, Christopher A. Thomas, Udo Hoss, Lei He, Michael R. Love
  • Patent number: 11162933
    Abstract: A system and method are directed to detecting the presence of heavy metals in a flowing fluid, such as a drinking water supply. The system includes a first chamber for receiving a filtered portion of the fluid and a second chamber for receiving an unfiltered portion of the fluid. A test device measures electrical properties of the filtered fluid in the first chamber and the unfiltered fluid in the second chamber, such as an electric potential difference, and a controller can use the measured electrical properties to detect the presence or quantity of one or more heavy metals within the fluid, such as lead, cadmium, zinc, nickel, and/or copper.
    Type: Grant
    Filed: September 11, 2019
    Date of Patent: November 2, 2021
    Assignee: Haier US Appliance Solutions, Inc.
    Inventors: Somak Chatterjee, Gregory Sergeevich Chernov, Andrew Reinhard Krause, Sharath Chandra Prasad, Praveena Alangar Subrahmanya
  • Patent number: 11160477
    Abstract: A diagnostic Electrochemical Impedance Spectroscopy (EIS) procedure is applied to measure values of impedance-related parameters for one or more sensing electrodes. The parameters may include real impedance, imaginary impedance, impedance magnitude, and/or phase angle. The measured values of the impedance-related parameters are then used in performing sensor diagnostics, calculating a highly-reliable fused sensor glucose value based on signals from a plurality of redundant sensing electrodes, calibrating sensors, detecting interferents within close proximity of one or more sensing electrodes, and testing surface area characteristics of electroplated electrodes. Advantageously, impedance-related parameters can be defined that are substantially glucose-independent over specific ranges of frequencies. An Application Specific Integrated Circuit (ASIC) enables implementation of the EIS-based diagnostics, fusion algorithms, and other processes based on measurement of EIS-based parameters.
    Type: Grant
    Filed: October 25, 2018
    Date of Patent: November 2, 2021
    Assignee: MEDTRONIC MINIMED, INC.
    Inventors: Ning Yang, Raghavendhar Gautham, Rajiv Shah
  • Patent number: 11154242
    Abstract: One aspect of the invention provides a method of determining metal ion levels in the patents due to corrosion and wear processes of the metallic implant in a human or veterinary patient. One embodiment provides a cost effective and patient driven early diagnostic method which has a potential application in orthopedics and dentistry. In one embodiment, the method includes the use of an electrochemical biosensor to detect metal ions or particles in a sample taken from a patient having a metallic implant.
    Type: Grant
    Filed: March 11, 2015
    Date of Patent: October 26, 2021
    Assignees: Rush University Medical Center, Board of Regents, The University of Texas System
    Inventors: Mathew T. Mathew, Shalini Prasad, Markus A. Wimmer, Nadim J. Hallab, Joshua Jacobs
  • Patent number: 11156582
    Abstract: This invention is based, in part, on our discovery of an essentially one-step, label-free system comprising a sensing unit having a redox current reporter and a nucleic acid sequence complementary to that of a target nucleic acid of interest or sufficiently complementary to that of the target nucleic acid or a sequence therein to specifically bind the target nucleic acid. The sensing unit is bound to an electroconductive substrate (e.g., a carbon- or metal-containing microelectrode (e.g., a gold microelectrode)), and the system includes a signal amplification mechanism that does not rely upon a redox enzyme and thereby overcomes a fundamental limitation of microelectrode DNA sensors that fail to generate detectable current in the presence of only small amounts of a target nucleic acid.
    Type: Grant
    Filed: July 6, 2016
    Date of Patent: October 26, 2021
    Assignees: GEORGIA STATE RESEARCH FOUNDATION, INC., THE UNITED STATES GOVERNMENT AS REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS
    Inventors: Gangli Wang, Tanyu Wang, Didier Merlin
  • Patent number: 11141116
    Abstract: Disclosed are systems and methods for generating graphical displays of analyte data and/or health information. In some implementations, the graphical displays are generating based on a self-referential dataset that are modifiable based on identified portions of the data. The modified graphical displays can indicate features in the analyte data of a host.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: October 12, 2021
    Assignee: DexCom, Inc.
    Inventors: Esteban Cabrera, Jr., Lauren Danielle Armenta, Scott M. Belliveau, Jennifer Blackwell, Leif N. Bowman, Rian Draeger, Arturo Garcia, Timothy Joseph Goldsmith, John Michael Gray, Andrea Jean Jackson, Apurv Ullas Kamath, Katherine Yerre Koehler, Paul Kramer, Aditya Sagar Mandapaka, Michael Robert Mensinger, Sumitaka Mikami, Gary A. Morris, Hemant Mahendra Nirmal, Paul Noble-Campbell, Philip Thomas Pupa, Eli Reihman, Peter C. Simpson, Brian Christopher Smith, Atiim Joseph Wiley
  • Patent number: 11119087
    Abstract: The invention provides a method for establishing a separable regeneration system for verifying regeneration effect between two antioxidants, which belongs to the field of regeneration effect of antioxidants. According to different solubilities of two antioxidants, lipid-soluble antioxidant is first combined into PE film, and water-soluble antioxidant is dissolved into deionized water and a separable regeneration system where antioxidants can contact with other but not dissolve in each other is formed. This method compares the differences of change of antioxidant capacity in aqueous phase with and without lipid-soluble antioxidant so that to judge whether the added lipid-soluble antioxidant has regeneration effect on aqueous-soluble antioxidant. The present invention effectively verifies the regeneration effect between different antioxidants, and has advantages of simple operation, less interference factors, intuitive and high efficiency.
    Type: Grant
    Filed: July 2, 2019
    Date of Patent: September 14, 2021
    Assignee: Jiangnan University
    Inventors: Hang Yu, Yaxin He, Yunfei Xie, Yahui Guo, Yuliang Cheng, Weirong Yao
  • Patent number: 11109784
    Abstract: Measurement of target analytes is carried out with an enzyme-based sensor. The enzyme hydrogel is protected by a porous layer of a metallic material. The size of the pores is small enough to prevent degradation of the enzyme layer caused by the immune system of an organism, but large enough to allow transfer of molecules that participate in the electrochemical reaction allowing the enzyme to detect the target analytes.
    Type: Grant
    Filed: November 14, 2018
    Date of Patent: September 7, 2021
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Dvin Adalian, Samson Chen, Muhammad M. Jilani, Axel Scherer, Xiomara Linnette Madero, Richard Smith
  • Patent number: 11091788
    Abstract: Embodiments of the present disclosure relate to NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Also provided are methods for making the compositions and for detecting and/or measuring analytes with NADP-dependent oxidoreductase compositions.
    Type: Grant
    Filed: March 2, 2017
    Date of Patent: August 17, 2021
    Assignee: Abbott Diabetes Care Inc.
    Inventors: Tianmei Ouyang, Benjamin J. Feldman
  • Patent number: 11069567
    Abstract: A metal interconnect structure can be fabricated within an integrated circuit (IC). A recess can be created in an IC dielectric layer and a surface modulation liner can be formed by depositing two different metallic elements onto the surfaces of the recess. One metallic element can have a standard electrode potential greater than a standard electrode potential of an interconnect metal, and the other metallic element can have a standard electrode potential less than the standard electrode potential of the interconnect metal. A metal interconnect structure can be formed by filling the remainder of the recess with interconnect metal, which is physically separated from the dielectric layer by the surface modulation liner. The surface topography of the metal interconnect structure can be modulated with a polishing process, by removing a top portion of the interconnect metal and a top portion of the surface modulation liner.
    Type: Grant
    Filed: June 26, 2019
    Date of Patent: July 20, 2021
    Assignee: International Business Machines Corporation
    Inventors: Conal Murray, Chih-Chao Yang
  • Patent number: 11041843
    Abstract: The invented organic memristor/memcapacitor device comprises arrayed cross-bar donut-shape toroidal matrix self-assembling membrane on an electrode mimicking mitochondria's double membrane surface structure and the direct electron-relay function, that used to mimic functions of Fibroblast Growth Factor Receptor 1 (FGFR1) and choline acetyltransferase (CHAT), which enables bio-communication signals flowing directly between the endotoxin interacted membrane electrode assembly (MEA) working electrode and a cathodic electrode; and from a biomarker Acetyl coenzyme A (AcCoA) interacted the MEA electrode and the cathodic electrode when applied a definite potential, respectively, for detection of a single E. coli cell and detection of a picomolar concentration of AcCoA under nature enzyme-free, antibody-free and reagent-free conditions. The sensor offers multiple functions for monitoring neuronal synapse pulse energy output.
    Type: Grant
    Filed: May 19, 2018
    Date of Patent: June 22, 2021
    Inventor: Ellen Tuanying Chen
  • Patent number: 11035819
    Abstract: A method for determining a concentration of an analyte in a fluidic sample is described. A sample is applied to a biosensor including an electrochemical cell having electrodes. A predetermined voltage waveform is applied during at least first and second time intervals. At least first and second current values are measured during the first and second time intervals, respectively. A turning point time is determined during the first time interval at which the measured first current values transition from a first to a second profile. The concentration of analyte in the sample is calculated based on determined turning point time and at least one measured current value. In another example, a physical characteristic of the sample is estimated based on measured current values. The concentration is calculated using a first or second model if the estimated physical characteristic of the sample is in a first or second range, respectively.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: June 15, 2021
    Assignee: LifeScan IP Holdings, LLC
    Inventors: Zuifang Liu, David McColl, Robert Donald, Anna Salgado, Antony Smith
  • Patent number: 11026664
    Abstract: Viruses, including corona viruses such as COVID-19, are often present in the mouth or nose of a person before they infect the body. The disclosed electrochemical device destroys or denatures viruses in the mouth or nose rendering the viruses ineffective to cause infection by applying a low voltage potential to the mucus. The device has a potentiostat powered by a small battery embedded in a plastic frame that fits in a person's mouth. The device has electrodes to contact the mucus of the mouth or nose. The mucus serves as electrolyte, conducting ions as well as viruses with their protein “skin” towards the electrode. The viruses are adsorbed onto the metal probes where the electrical current denatures the protein skin of the virus rendering the virus ineffective to cause infection.
    Type: Grant
    Filed: April 22, 2020
    Date of Patent: June 8, 2021
    Inventor: Slobodan Petrovic
  • Patent number: 11006870
    Abstract: An apparatus for insertion of a medical device in the skin of a subject is provided.
    Type: Grant
    Filed: September 1, 2020
    Date of Patent: May 18, 2021
    Assignee: ABBOTT DIABETES CARE INC.
    Inventors: Phillip Yee, Christopher A. Thomas, Udo Hoss, Lei He, Michael R. Love
  • Patent number: 11006871
    Abstract: An apparatus for insertion of a medical device in the skin of a subject is provided.
    Type: Grant
    Filed: September 1, 2020
    Date of Patent: May 18, 2021
    Assignee: ABBOTT DIABETES CARE INC.
    Inventors: Phillip Yee, Christopher A. Thomas, Udo Hoss, Lei He, Michael R. Love
  • Patent number: 10989611
    Abstract: Methods and systems accurately determine an analyte concentration in a fluid sample. In an example embodiment, a receiving port receives a test sensor. The test sensor includes a fluid-receiving area for receiving a fluid sample. The fluid-receiving area contains a reagent that produces a measurable reaction with an analyte in the fluid sample. The test sensor has a test-sensor temperature and the reagent has a reagent temperature. A measurement system measures the reaction between the reagent and the analyte. A temperature-measuring system measures the test sensor temperature when the test sensor is received into the receiving port. A concentration of the analyte in the fluid sample is determined according to the measurement of the reaction and the measurement of the test sensor temperature. A diagnostic system determines an accuracy of the temperature-measuring system. The calculation of the analyte concentration may be adjusted according to the accuracy of temperature-measuring system.
    Type: Grant
    Filed: January 15, 2019
    Date of Patent: April 27, 2021
    Assignee: Ascensia Diabetes Care Holdings AG
    Inventors: Narasinha Parasnis, Hoi-Cheong Steve Sun, Mu Wu
  • Patent number: 10982251
    Abstract: A method of making an electrochemical sensor strip that includes: depositing a first electrode on a base; depositing a second electrode on the base; applying a first layer onto the first electrode; and applying a second layer onto the second electrode. The first layer includes an oxidoreductase and a mediator. The second layer includes a soluble redox species.
    Type: Grant
    Filed: September 27, 2019
    Date of Patent: April 20, 2021
    Assignee: ASCENSIA DIABETES CARE HOLDINGS AG
    Inventors: Huan-Ping Wu, Greg P. Beer, Christina Blaschke
  • Patent number: 10983088
    Abstract: The invention provides novel microfluidic coulometric sensors having a silver (Ag) band electrode longitudinally placed in a microchannel affording visual readout suitable for the naked eye, and methods of fabrication and applications thereof.
    Type: Grant
    Filed: March 28, 2017
    Date of Patent: April 20, 2021
    Assignee: University of Massachusetts
    Inventors: Kwok-Fan Chow, Jung Min Oh, Mahadurage Sachintha Wijesinghe
  • Patent number: 10948399
    Abstract: A determination method includes: using a microchip, including a capillary flow path and a sample reservoir connected to the capillary flow path at an upstream side, to fill the capillary flow path with a first solution for electrophoresis, and supply the sample reservoir with a second solution containing an analyte; applying a voltage between the sample reservoir supplied with the second solution and the inside of the capillary flow path filled with the first solution, to move a component contained in the second solution in the capillary flow path and separate the component in the capillary flow path; optically detecting a value related to a component difference between the first solution and the second solution, other than a value related to the analyte, for the separated component; and determining whether the optical detection is favorable or poor by comparing the optically detected value with a predetermined threshold value.
    Type: Grant
    Filed: October 17, 2018
    Date of Patent: March 16, 2021
    Assignee: ARKRAY, Inc.
    Inventor: Takanari Shigemitsu
  • Patent number: 10947328
    Abstract: A non-leaching mediator may include a polymer having a polymeric backbone, and a plurality of phenothiazine groups bonded to the polymeric backbone. The plurality of phenothiazine groups may include at least one of a phenothiazine group having the general formula (IV): and salts thereof, where n is about 9 and “R” represents the polymeric backbone to which the phenothiazine group is bonded, and a phenothiazine group having the general formula (V): and salts thereof, where n is about 9 and “R” represents the polymeric backbone to which the phenothiazine group is bonded.
    Type: Grant
    Filed: April 27, 2020
    Date of Patent: March 16, 2021
    Assignee: Ascensia Diabetes Care Holdings AG
    Inventors: Jiangfeng Fei, William Chiang, Frank Kerrigan, Stuart Green, Craig Robson, Howard Easterfield
  • Patent number: 10905362
    Abstract: A universal electrochemical micro-sensor can be used either as a biosensor or an environmental sensor. Because of its small size and flexibility, the micro-sensor is suitable for continuous use to monitor fluids within a live subject, or as an environmental monitor. The micro-sensor can be formed on a reusable glass carrier substrate. A flexible polymer backing, together with a set of electrodes, forms a reservoir that contains an electrolytic fluid chemical reagent. During fabrication, the glass carrier substrate protects the fluid chemical reagent from degradation. A conductive micromesh further contains the reagent while allowing partial exposure to the ambient biological or atmospheric environment. The micromesh density can be altered to accommodate fluid reagents having different viscosities. Flexibility is achieved by attaching a thick polymer tape and peeling away the micro-sensor from the glass carrier substrate.
    Type: Grant
    Filed: May 8, 2019
    Date of Patent: February 2, 2021
    Assignee: STMICROELECTRONICS PTE. LTD.
    Inventors: Olivier Le Neel, Suman Cherian, Calvin Leung
  • Patent number: 10890557
    Abstract: The present disclosure relates to an electron transfer mediator comprising the osmium complex or a salt thereof, a reagent composition for an electrochemical biosensor, and an electrochemical biosensor, where the osmium compound or its salt maintains a stable oxidation-reduction form for an extended time period, a capacity to react with oxidoreductase being capable of performing the redox reaction of the target analytes, and no effect of oxygen partial pressure.
    Type: Grant
    Filed: December 31, 2015
    Date of Patent: January 12, 2021
    Assignee: I-SENS, INC.
    Inventors: In Jun Yoon, Jae Ho Shin, Yeon Ho Jung, Geun Sig Cha, Hakhyun Nam
  • Patent number: 10845330
    Abstract: A physiological characteristic sensor, a method for forming a physiological characteristic sensor, and a method for forming a platinum deposit having a rough surface are presented here. The method for forming a physiological characteristic sensor includes immersing a sensor electrode in a platinum electrolytic bath. Further, the method includes performing an electrodeposition process by sequentially applying a pulsed signal to the sensor electrode, wherein the pulsed signal includes a repeated cycle of a first current and a second current different from the first current, and applying a non-pulsed continuous signal to the sensor electrode, wherein the non-pulsed continuous signal includes a non-repeated application of a third current, to form a platinum deposit on the sensor electrode.
    Type: Grant
    Filed: May 15, 2017
    Date of Patent: November 24, 2020
    Assignee: Medtronic MiniMed, Inc.
    Inventors: Steven C. Jacks, Raghavendhar Gautham, Bradley C. Liang, Megan E. Little, Daniel E. Pesantez, Rajiv Shah
  • Patent number: 10843192
    Abstract: At least one electrode is integrated on a lab on a chip cartridge in a sample preparation chamber of the cartridge, a DNA hybridization chamber of the cartridge, a protein assay chamber of the cartridge, and/or a detection chamber of the cartridge, for example, where the electrode is used to generate pH electrochemically in order to activate, deactivate, or intermediately attenuate an enzyme's activity on demand, in order to increase the fidelity of analyte detection, for cell lysis, for protein extraction, for DNA dehybridization, for primer hybridization control, for sample pre concentration, and/or for washing to remove non target species.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: November 24, 2020
    Assignee: ROBERT BOSCH GMBH
    Inventors: Nadezda Fomina, Christopher Johnson, Habib Ahmad, Christoph Lang, Franz Laermer
  • Patent number: 10833162
    Abstract: A system and method for fabricating at least one of, a molecular device element and a TBELMD including depositing a first electrode material on an insulating substrate or layer, performing a photolithography process in the first electrode material, creating a trench component in the first electrode material with the photolithography process, determining a section of the electrode material to remove based on at least one of, a molecular device element and a TBELMD to be produced, removing the section of said first electrode material, oxidizing a portion of the first electrode material, creating a first insulator part from the oxidized portion of the first electrode material, in which the oxidized portion of the first electrode material includes at least a first electrode metal surface, depositing a second electrode material, and bridging the first and second electrode material.
    Type: Grant
    Filed: August 14, 2018
    Date of Patent: November 10, 2020
    Inventor: Pawan Tyagi
  • Patent number: 10830724
    Abstract: The present application provides devices, systems and methods for detecting the presence and/or length of an analyte. More specifically, the present application is directed to a structure and system that includes a micro-capacitive sensor array for detecting the presence of an analyte in a sample and determining the length and/or composition of an analyte, such as a nucleic acid, as well as methods for using the same.
    Type: Grant
    Filed: December 22, 2017
    Date of Patent: November 10, 2020
    Assignee: International Business Machines Corporation
    Inventors: Frank R. Libsch, Venkat K. Balagurusamy
  • Patent number: 10816545
    Abstract: An automated method of evaluating a collected fluid sample includes: filling a sample cavity with the collected fluid sample; adding a buffer solution; separating the collected fluid sample into a first portion and a second portion; mixing the second portion with tagged antibodies; removing leftover tagged antibodies; and measuring a difference between the first portion and the second portion. A sample collection and testing device includes: a reference cavity comprising a reference fluid sample; a test cavity comprising a test fluid sample; a reference measurement element associated with the reference cavity; and a test measurement element associated with the test cavity. A method of evaluating a collected fluid sample including: separating the sample; pumping a first portion to a first measurement cavity; adding a solution to a second portion and pumping the mixture to a second measurement cavity; and measuring a charge difference between the first and second measurement cavities.
    Type: Grant
    Filed: April 23, 2018
    Date of Patent: October 27, 2020
    Assignee: 2Pi-Sigma Corporation
    Inventor: Mehdi Hatamian
  • Patent number: 10794852
    Abstract: This disclosure describes, in one aspect, a device for electrochemical quantitation of autoantibodies. Generally, the device includes a housing that defines a plurality of channels and at least two reaction zones. A first reaction zone includes a porous membrane and a first electrode assembly in fluid communication with a first channel. The first reaction zone also includes a first plurality of autoantigens immobilized to the porous membrane. The first electrode assembly is in communication with an amperometric reader. A second reaction zone includes a porous membrane and a second electrode assembly in fluid communication with a second channel. The second reaction zone includes a second plurality of autoantigens immobilized to the porous membrane. The second electrode assembly is in communication with the amperometric reader. Finally, the device includes a source of negative pressure in fluid communication with the first reaction zone and the second reaction zone.
    Type: Grant
    Filed: January 29, 2015
    Date of Patent: October 6, 2020
    Inventors: Robert L. Rubin, Konstantin N. Konstantinov, David A. Wall
  • Patent number: 10788442
    Abstract: An electrochemical test device for use in determining a concentration of each of a first analyte and a second analyte in a fluid sample is provided. The electrochemical test device comprises a set of electrodes including a first working electrode having sensing chemistry for the first analyte and a second working electrode having sensing chemistry for the second analyte, wherein the first analyte is lactate and the sensing chemistry for the lactate comprises lactate oxidase and an electron transfer agent, and wherein the sensing chemistry for the second analyte comprises a diaphorase, an electron transfer agent, an NAD(P)+-dependent dehydrogenase and a cofactor for the NAD(P)+-dependent dehydrogenase.
    Type: Grant
    Filed: April 27, 2016
    Date of Patent: September 29, 2020
    Assignee: INSIDE BIOMETRICS INTERNATIONAL LIMITED
    Inventors: Marco Cardosi, Stephanie Kirkwood, Damian Baskeyfield
  • Patent number: 10788441
    Abstract: A measuring method of measuring a concentration of a target ingredient in a sample by using a sensor including an interdigitated array electrode that includes a first electrode and a second electrode, and a reagent layer on the interdigitated array electrode, the measuring method includes: applying a voltage to between the first electrode and the second electrode; measuring a first current value of an electric current flowing between the first electrode and the second electrode; measuring a second current value of the current flowing between the first electrode and the second electrode; calculating the concentration of the target ingredient in the sample, based on a third current value; calculating a correction value, based on the first current value and the second current value; and a step of correcting the concentration of the target ingredient in the sample, based on the correction value.
    Type: Grant
    Filed: August 9, 2016
    Date of Patent: September 29, 2020
    Assignees: ARKRAY, Inc., Ultizyme International Ltd.
    Inventors: Hisashi Kaneda, Yasuhide Kusaka, Koji Sode
  • Patent number: 10775331
    Abstract: In a biological information measurement device, the ingress of biological sample is detected even when a sensor is mounted, which reduces measurement error. The biological information measurement device comprises a voltage supply component that supplies voltage between first and second connectors, between second and third connectors, and between first and third connectors, a current measurement component that measures a first current that flows between the first and second connectors, a second current that flows between the second and third connectors, and a third current that flows between the first and third connectors, and a controller that is connected to the current measurement component and the voltage supply component. The controller compares two or more of the currents and thereby determines whether a foreign substance has adhered between two or more connectors out of the first, second, and third connectors.
    Type: Grant
    Filed: May 23, 2017
    Date of Patent: September 15, 2020
    Assignee: PHC HOLDINGS CORPORATION
    Inventors: Mamiko Ochi, Takashi Miki, Daiki Mizuoka
  • Patent number: 10768177
    Abstract: A biosensor for detecting bacteria may use bacteriophages in a sandwich-assay system. The biosensor may include a capture element and a detection element. The capture element may include a substrate and a bacteriophage. The detection element may include a bacteriophage and a signal amplification element. The biosensor may be utilized such that the target bacterium is sandwiched between the capture element and the detection element, and a quantifiable signal may be generated to measure the amount of bacteria in a sample. The biosensor of the present invention utilizes direct sensing to detect the bacteria in the sample as opposed to indirect sensing methods.
    Type: Grant
    Filed: August 29, 2018
    Date of Patent: September 8, 2020
    Assignee: The United States of America, as represented by the Secretary of Agriculture
    Inventors: Vivian C. Wu, Irwin Quintela
  • Patent number: 10758904
    Abstract: A particle manipulation device includes a substrate and a microchannel included in the substrate and configured to receive a fluid including particles therein. A biasing structure is formed on the substrate adjacent to, but outside the microchannel. The biasing structure is configured to dispense radiation at a frequency to bias movement of the particles within the microchannel from outside the microchannel.
    Type: Grant
    Filed: June 16, 2017
    Date of Patent: September 1, 2020
    Assignee: International Business Machines Corporation
    Inventor: Jaione Tirapu Azpiroz
  • Patent number: 10732139
    Abstract: A glucose sensor suitable for measuring glucose levels in human saliva is provided. Systems containing the glucose sensor and methods for making and using the sensor are also provided. The glucose sensor is highly sensitive and can detect glucose levels at least down to 5 ppm. Fabrication of the sensor involves depositing single-walled carbon nanotubes onto the surface of a working electrode in a 3 electrode electrochemical detector and functionalizing the nanotubes by depositing layers of polymers, metallic nanoparticles, and glucose oxidase enzyme onto the nanotubes. The sensor can be used as a disposable, single-use device or as part of an analytical system, such as a microfluidics system, for the analysis of multiple analytes. The sensor enables the diagnosis and monitoring of diabetes to be performed without pain or the need for finger pricks in a home or clinical setting.
    Type: Grant
    Filed: December 14, 2015
    Date of Patent: August 4, 2020
    Assignee: Northeastern University
    Inventors: Wenjun Zhang, Ming L. Wang
  • Patent number: 10731209
    Abstract: Methods of loading a molecule of interest into a sample well are provided. In some aspects, methods of loading a molecule of interest into a sample well involve loading a molecule of interest into a sample well in the presence of a crowding agent and/or a condensing agent. In some aspects, methods of loading a sequencing template into a sample well are provided.
    Type: Grant
    Filed: December 19, 2017
    Date of Patent: August 4, 2020
    Assignee: Quantum-Si Incorporated
    Inventors: James A. Ball, Jeremy Lackey, Brian Reed, Alexander Goryaynov, Jonathan M. Rothberg, Thomas Christian
  • Patent number: 10641724
    Abstract: A sampling plate (1) is provided comprising a sample zone (2) for receiving a liquid sample, and two drive electrodes (3, 4) with separate respective electrode terminals spaced by a spacing for receiving a the liquid sample within the sample zone for use in driving an electrical signal through the sample. Two sensing electrodes (5, 6) are provided with separate respective electrode terminals spaced between the electrode terminals of the two drive electrodes for use in sensing an electrical signal generated by the drive electrodes within a the sample. A sampling apparatus (15) is provided for use with the plate.
    Type: Grant
    Filed: April 12, 2013
    Date of Patent: May 5, 2020
    Assignee: SMARTCASE TECHNOLOGIES LIMITED
    Inventors: Phillip J. Ainger, Matthew Robert Bryan
  • Patent number: 10627397
    Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. In this assay, the amount of the signal or the first member of the second binding pair bound to the binding agent is inversely proportional to the analyte in the sample.
    Type: Grant
    Filed: April 9, 2019
    Date of Patent: April 21, 2020
    Assignee: Enzo Life Sciences, Inc.
    Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
  • Patent number: 10520460
    Abstract: A method for determining a diffusion feature of a fluidic sample using redox reactions in an electrochemical cell that has at least two electrodes, wherein the first electrode has at least one redox mediator at its surface or in close vicinity of its surface, and the second electrode has an electrode surface free of the redox mediator(s) in the beginning of a test, the method comprising: applying an electric potential to a fluidic sample in the electrochemical cell to initiate redox reactions at the two electrode surfaces; measuring current associated with the applied potential as a function of time, and using a measurement point on a transient part of the measured current at or after a turning point and its associated time to determine the diffusion feature.
    Type: Grant
    Filed: October 26, 2015
    Date of Patent: December 31, 2019
    Assignee: LifeScan IP Holdings, LLC
    Inventors: Zuifang Liu, Magdalena Gutowska
  • Patent number: 10465229
    Abstract: A detection system for determining pyruvate dehydrogenase (PDH) levels in a bodily sample includes at least one reaction solution for generating NAD+ upon combination with PDH in the bodily sample, the reaction solution including pyruvate and NADH and a biosensor for determining the level of generated NAD+.
    Type: Grant
    Filed: May 27, 2015
    Date of Patent: November 5, 2019
    Assignee: CASE WESTERN RESERVE UNIVERSITY
    Inventor: Chung-Chiun Liu