Abstract: The disclosure provides a method of generating an artificial fluorescent image of cells is provided.

Abstract: One example system comprises a LIDAR sensor that rotates about an axis to scan an environment of the LIDAR sensor. The system also comprises one or more cameras that detect external light originating from one or more external light sources. The one or more cameras together provide a plurality of rows of sensing elements. The rows of sensing elements are aligned with the axis of rotation of the LIDAR sensor. The system also comprises a controller that operates the one or more cameras to obtain a sequence of image pixel rows. A first image pixel row in the sequence is indicative of external light detected by a first row of sensing elements during a first exposure time period. A second image pixel row in the sequence is indicative of external light detected by a second row of sensing elements during a second exposure time period.

Abstract: A microscope system is provided with a microscope that acquires images at least at a first magnification and a second magnification higher than the first magnification, and a processor. The processor is configured to specify a type of a container in which a specimen is placed, and when starting observation of the specimen placed in the container at the second magnification, the processor is configured to specify an observation start position by performing object detection according to the type of container on a first image that includes the container acquired by the microscope at the first magnification, and control a relative position of the microscope with respect to the specimen such that the observation start position is contained in a field of view at the second magnification of the microscope.

Abstract: A microscope includes a digital imaging device. The digital imaging device comprises a processor which is configured to: obtain a number of digital grayscale images of an object at a number of different spectral sensitivities, each of the digital grayscale images including grayscale information based on a different one of the different spectral sensitivities, obtain a number of weighting factors for each of the digital grayscale images, the weighting factors being allocated to a number of color channels defining a predetermined color space, and synthesize a digital color image of the object from the digital grayscale images in the color space by distributing the grayscale information of each grayscale image over the color channels in accordance with the weighting factors.

Abstract: A light emitting device including at least one LED stack, electrode pads disposed on the LED stack, and cantilever electrodes disposed on the electrode pads, respectively, in which each of the cantilever electrodes has a fixed edge that is fixed to one of the electrode pads and a free standing edge that is spaced apart from the one of the electrode pads.

Abstract: A microscope includes an illumination unit for illuminating a region of a specimen to generate an illuminated region, an imaging optical unit for magnified imaging of the illuminated region, an image sensor disposed downstream of the imaging optical unit for capturing the magnified image of the illuminated region, a camera for recording an overview region of the specimen without using the imaging optical unit and a control unit for controlling the image sensor and the camera. The overview region includes a part of the illuminated region and a non-illuminated region of the specimen. The control unit actuates the camera to make a recording of the overview region. The control unit actuates the image sensor to cause a recordation of the magnified image of the illuminated region. The control unit generates an overview image based on the recording of the overview region and the recording of the magnified image.

Abstract: A microscope comprises a microscope stand, a camera for recording microscope images and a computing device, which is configured to carry out image processing of the recorded microscope images. The computing device is configured to: define relevant image structures; localize relevant image structures in the microscope images; derive stitching parameters from locations of the relevant image structures; and create a result image with the aid of the microscope images, with the stitching parameters being taken into account. Moreover, a corresponding method is described.

Abstract: An adapter which controls rotation of a microscope on which a slide is placed and an imaging unit and which easily performs correction of a rotation shift is provided. The adapter includes a first connection member connected to a microscope, a second connection member connected to an imaging unit, a rotation member arranged between the first and second connection members and configured to rotate the second connection member relative to the first connection member using optical axes of the microscope and the imaging unit at a center, a control member configured to be fixed on one of the first and second connection members and control the rotation of the connection member, and a driving member configured to be engaged with the first connection member or the second connection member and change a position of the second connection member relative to the first connection member around the optical axes.

Abstract: Even if a generated wide-field image includes residual local misalignment, this charged particle microscope device can prompt for user input to correct such local misalignment, and can regenerate, on the basis of the user input, a wide-field image that includes little misalignment even in local areas of the overlap regions thereof.

Abstract: There is provided a medical observation apparatus including: a determination unit which determines an apparatus to be used based on a captured image for medical use captured by an imaging device; and a display control unit which causes a related image for medical use corresponding to the determined apparatus and the captured image for medical use to be displayed.

Abstract: An imaging system includes a sample mount for holding a sample to be imaged, a light source configured to emit a light beam to be incident on the sample, a translation mechanism coupled to the sample mount and configured to scan the sample to a plurality of sample positions in a plane substantially perpendicular to an optical axis of the imaging system, a mask positioned downstream from the sample along the optical axis, and an image sensor positioned downstream from the mask along the optical axis. The image sensor is configured to acquire a plurality of images as the sample is translated to the plurality of sample positions. Each respective image corresponds to a respective sample position. The imaging system further includes a processor configured to process the plurality of images to recover a complex profile of the sample based on positional shifts extracted from the plurality of images.

Abstract: A medical image processing apparatus of an embodiment includes processing circuitry. The processing circuitry is configured to acquire time-series medical images including blood vessels of an examination subject, the time-series medical images being fluoroscopically captured in at least one direction at a plurality of points in time, generate a blood vessel shape model including time-series variation information about the blood vessels in an analysis region of the blood vessels on the basis of the acquired time-series medical images, and perform fluid analysis of blood flowing through the blood vessels on the basis of the generated blood vessel shape model.

Abstract: The present disclosure relates to systems and methods for facing a tissue block. In some embodiments, a method is provided for facing a tissue block that includes imaging a tissue block to generate imaging data of the tissue block, the tissue block comprising a tissue sample embedded in an embedding material, estimating, based on the imaging data, a depth profile of the tissue block, wherein the depth profile comprises a thickness of the embedding material to be removed to expose the tissue sample to a pre-determined criteria, and removing the thickness of the embedding material to expose the tissue to the pre-determined criteria.

Abstract: An image processing method includes: a fluorescent image capturing step of capturing a fluorescent image of a fluorescently labeled tissue specimen; a creating step of creating a fluorescent whole slide image based on the captured fluorescent image; and a storing step of storing the created fluorescent whole slide image. The tissue specimen is fluorescently labelled by using, as a staining reagent, fluorescent substance integrated nanoparticles obtained by bonding a biological substance recognition part to fluorescent particles on which a plurality of fluorescent substances are integrated.

Abstract: A method is disclosed for acquiring a single, in-focus two-dimensional projection image of a live, three-dimensional cell culture sample, with a fluorescence microscope. One or more long-exposure “Z-sweep” images are obtained, i.e. via a single or series of continuous acquisitions, while moving the Z-focal plane of a camera through the sample, to produce one or more two-dimensional images of fluorescence intensity integrated over the Z-dimension. The acquisition method is much faster than a Z-stack method, which enables higher throughput and reduces the risk of exposing the sample to too much fluorescent light. The long-exposure Z-sweep image(s) is then input into a neural network which has been trained to produce a high-quality (in-focus) two-dimensional projection image of the sample.

Abstract: A method for observing a sample is placed between a light source and an image sensor, comprising at least 10000 pixels, the light source emits an illuminating beam, which propagates to the sample, the light beam is emitted in an illumination spectral band (??11) lying above 800 nm, the method comprising the following steps: (a) illuminating the sample with the light source; (b) acquiring an image of the sample (I0) with the image sensor, no image-forming optics being placed between the sample and the image sensor; and (c) the image sensor being configured such that it has a detection spectral band (??20), which blocks wavelengths in the visible spectral band, such that the image may be acquired in ambient light.

Abstract: A microscopy system includes a microscope, a stand configured to mount the microscope and including a drive device configured to move the microscope, a detection device configured to detect a spatial position of a target fastened to a body part or to an instrument, wherein the position detection device includes the target with at least one marker element and an image capture device configured to optically capture the target. The microscopy system further includes at least one control device configured to operate the microscopy system according to the detected position of the target, wherein the position detection device is configured to determine the position of the target by evaluating a two-dimensional image of the image capture device. In addition, a method for operating the microscopy system is provided.

Abstract: Systems and methods for classifying and/or sorting T cells by activation state are disclosed. The system includes a cell classifying pathway, a single-cell autofluorescence image sensor, a processor, and a non-transitory computer-readable memory. The memory is accessible to the processor and has stored thereon a trained convolutional neural network and instructions. The instructions, when executed by the processor, cause the processor to: a) receive the autofluorescence intensity image; b) optionally pre-process the autofluorescence intensity image to produce an adjusted autofluorescence intensity image; c) input the autofluorescence intensity image or the adjusted autofluorescence intensity image into the trained convolutional neural network to produce an activation prediction for the T cell.

Abstract: A particle velocimetry system and algorithm are provided for executing a particle reconstruction to determine three-dimensional positions of particles in a particle laden fluid flow from two-dimensional flow images generated by two-dimensional image sensors; generate a three-dimensional particle distribution from the three-dimensional position; and execute a recursive loop for performing further iterations of particle reconstruction and generation of three-dimensional particle distributions, with recursive iterations of particle reconstruction executed with the use of particle property data obtained from the prior executed iteration of particle reconstruction.

Abstract: A stand for a surgical microscope includes a digiscope and an imaging unit which are interconnected and mounted on an arm of the stand at a joint rotatably about an axis of rotation. The digiscope and the imaging unit are arranged on a bridge such that a fixed spatial relationship, in particular a distance larger than zero between the digiscope and the imaging unit during a relative movement between the digiscope and the patient is maintained. A method for configuring a stand with a bridge includes determining distances between eyes of a surgeon and the imaging unit, between the surgeon and an operating field, between the receptacle of the digiscope and the operating field, and between the imaging unit and the receptacle of the digiscope, and determining a length of the bridge to optimally set a distance from the surgeon to the patient and the imaging unit for the surgeon.

Abstract: The method includes receiving a digital image of a slide, the slide including the tissue sample, a target dot and a fluoro dot. The digital image includes intensity values of the tissue sample, the target dot and the fluoro dot. The method further includes receiving pixel intensities of the fluoro dot depicted in the image; pixel intensities of the target dot depicted in the image; pixel intensities of an area of the tissue sample depicted in the image; fluorescence-inducing molecule density information indicative of the known density of the fluorescence-inducing molecules in the fluoro dot; target molecule density information indicative of the known density of the target molecules in the target dot; and computing the density of target molecules in the area of the tissue sample as a function of the received pixel intensities and the received molecule density information.

Abstract: The present invention relates to computing-implemented method and system that improves matrix multiplication efficiency, especially to method and system optimizing matrix multiplication using sparse basis approach. Matrices to be multiplied are organized into specially ordered vectors with zero values, facilitates speed up during linear combination computation or synthesis process.

Abstract: Provided are a method for determining whether an examinee is infected by microorganism, and a determination apparatus using the same. Specifically, the determination apparatus according to the present invention obtains an microphotographed image of a biological sample of the examinee; receives the obtained microphotographed image and generates analysis information on the microorganism based on a deep learning model of the examinee; visualize the generated analysis information to provide it, so as to perform at least one of (i) a process of supporting a remote reading on whether the microorganism corresponding to the analysis information exists or not, and (ii) a process of supporting a user of the computing apparatus to read whether the microorganism corresponding to the analysis information exists or not; and provides a final result as its result.

Abstract: A ptychography system is presented for imaging an object located in an object plane. The ptychography system comprises an optical system, and a detection device. The optical system comprises a single shot ptychography arrangement configured and operable to create light response patterns from the object in the object plane on a pixel matrix of the detection device during the same exposure session of the detection device, wherein the optical system further comprises at least one light coding device configured and operable to apply at least one predetermined coding function to at least one of illuminating light and the light response of the object being collected, and said detection device is configured and operable with a predetermined duration of the exposure session during which the pixel matrix detects the collected light, such that image data indicative of the detected light during a single exposure session is in the form of a coded light response of the object being illuminated.

Abstract: An optical inspection system includes a brightness inspection module for inspecting the brightness of a light emitting element, an integrated inspection module for inspecting the near field optical characteristic and the beam quality factor of the light emitting element, and a far field inspection module for inspecting the far field optical characteristic of the light emitting element. As a result, the optical inspection system is space-saving and capable of reducing the distance and time of the movement of the device under test.

Abstract: A fluorescence image analyzer, analyzing method, and pretreatment evaluation method capable of determining with high accuracy whether a sample is positive or negative are provided. A pretreatment part performs pretreatment including a step of labeling a target site with a fluorescent dye to prepare a sample. A fluorescence image analyzer measures and analyzes the sample. The fluorescent image analyzer includes light sources to irradiate light on the sample, imaging part to capture the fluorescent light given off from the sample irradiated by light, and processing part for processing the fluorescence image captured by the imaging part. The processing part extracts the bright spot of fluorescence generated from the fluorescent dye that labels the target site from the fluorescence image for each of a plurality of cells included in the sample, and generates information used for determining whether the sample is positive or negative based on the bright spots extracted for each of the plurality of cells.

Abstract: A medical/surgical microscope with two cameras configured to capture two dimensional images of specimens being observed. The medical/surgical microscope is secured to a control apparatus configured to adjust toe-in of the two cameras to insure the convergence of the images. The medical/surgical microscope includes a computer system with a non-transitory memory apparatus for storing computer program code configured for digitally rendering real-world medical/surgical images. The medical/surgical microscope has an illumination system with controls for focusing and regulating the lighting of a specimen. The medical/surgical microscope is configured for real-time video display with the function of recording and broadcasting simultaneously during surgery.

Abstract: Various optical systems equipped with diode laser light sources are discussed in the present application. One example system includes a diode laser light source for providing a beam of radiation. The diode laser has a spectral output bandwidth when driven under equilibrium conditions. The system further includes a driver circuit to apply a pulse of drive current to the diode laser. The pulse causes a variation in the output wavelength of the diode laser during the pulse such that the spectral output bandwidth is at least two times larger the spectral output bandwidth under the equilibrium conditions.

Abstract: The specimen treatment and measurement system 2000 according to the present invention is provided with: a movement stage 2300 having a plurality of treatment lanes for treating specimens in parallel; a consumables supply module 2100 for storing consumables for use in treatment of the specimens, and supplying the consumables to the movement stage 2300; a cartridge supply module 2500 for storing cartridges for use in treatment of the specimens, and supplying the cartridges to the movement stage 2300; and a stage transfer mechanism 2400 for transferring the movement stage 2400 to each module. The cartridge supply module 2500 has a plurality of cartridge cartons for accommodating the cartridges stacked on top of each other, and a push-out mechanism for pushing cartridges out of a cartridge carton to a supply position of the cartridge supply module 2500.

Abstract: The invention relates to an illumination apparatus for a microscope, a microscope and a method for operating the illumination apparatus. The illumination apparatus has a sample space for holding a sample that is to be illuminated, and at least one laser light source. An objective for the directional emission of laser radiation of a first wavelength along a first optical axis that is directed into the sample space, and with a cover of the sample space by which the sample space is delimited at least on one of its sides. The cover further has a layer that is either impenetrable for the laser radiation over a blocking angle range of the illumination angle and is transmissive for radiation of a second wavelength over a transmitted light angle range, or has a controllable layer that, in a first control state, is transparent for radiation of the second wavelength and, in a second control state, is impenetrable for the laser radiation of the first wavelength.

Abstract: Disclosed is a shooting method, which is applied to a terminal device defining at least one camera, and the at least one camera includes at least one lift-able camera. The method includes: displaying a shooting interface; receiving a shooting instruction; controlling a first camera to shoot at least two images at different heights to obtain a shot photo, where the first camera is one of the at least one lift-able camera. With adoption of the embodiments of this application, the software debugging time for the camera can be reduced, and the shooting efficiency can be improved.

Abstract: The present disclosure provides a method for controlling autonomous microscope system, microscope system, and computer readable storage medium. Taking the advantage of a neural network trained in a reinforcement learning scheme, the method automatizes the analysis process of biological sample executed by microscope system and therefore improves the diagnosis efficiency.

Abstract: A system includes an imaging optical system that forms an optical image of an observed object; a control apparatus that switches between a superresolution observation mode and a normal observation mode; and a rotatable disk located at a position conjugate to a front focal position of the imaging optical system and having a plurality of apertures. The imaging optical system changes a projection magnification of an intermediate image that is a point image of a portion of the observed object that is formed on the disk. The control apparatus sets, during the superresolution observation mode, the projection magnification in a manner such that the intermediate image becomes at least twice as large as the apertures and sets, during the normal observation mode, the projection magnification in a manner such that the projection magnification becomes lower than the projection magnification in the superresolution observation mode.

Abstract: A semiconductor device package includes a supporting element, a transparent plate disposed on the supporting element, a semiconductor device disposed under the transparent plate, and a lid surrounding the transparent plate. The supporting element and the transparent plate define a channel.

Abstract: Keeping the excitation light sheet in focus is critical in selective plane illumination microscopy (SPIM) to ensure its 3D imaging ability. Unfortunately, an effective method that can be used in SPIM on general biological specimens to find the axial position of the excitation light sheet and keep it in focus is barely available. Here, we present a method to solve the problem. We investigate its mechanism and demonstrate its performance on a lattice light sheet microscope.

Abstract: A high resolution inspection apparatus using a terahertz Bessel beam. The high resolution inspection apparatus comprises a terahertz wave generating unit for generating a terahertz wave; a Bessel beam forming unit for generating a terahertz Bessel beam using the terahertz wave incident from the terahertz wave generating unit; a ring beam forming unit for forming a ring beam using the terahertz Bessel beam and concentrating the formed ring beam to an inspection target object; a scattered light detecting unit for detecting scattered light generated from the inspection target object; and a ring beam detecting unit for detecting a ring beam transmitted through the inspection target object.

Abstract: The invention relates to a method for determining an imaging function of a mask inspection microscope, wherein the mask inspection microscope comprises an imaging optical element, a tube, a recording device, an object stage, an illumination unit for measurement with transmitted light and an illumination unit for measurement in reflection, comprising the following method steps: a) measuring the intensities in the pupil plane of the imaging optical element in a reflective measurement, b) measuring the intensities in the pupil plane of the imaging optical element in a transmitted-light measurement, d) Determining the imaging function of the intensities of the imaging optical element, d) determining the imaging function of the intensities of the illumination optical element comprised in the illumination unit for the transmitted-light measurement.

Abstract: A system and a method for measuring the focus state of an optical instrument are described. The system and the method provide for forming an image of the exit pupil of the objective of the optical instrument. Where the image of the exit pupil is formed, one or more optical elements are placed, deviating at least part of the rays coming from the exit pupil, so that rays coming from different non-overlapping portions of the pupil follow separate optical paths. The rays coming from the two portions of the pupil are then focused, in order to obtain two bidimensional images. A computer determines the mutual distance between these two bidimensional images as a mutual rigid lateral displacement between the two and, based on this distance, determines the corresponding defocus of the optical instrument.

Abstract: A certain material irregularly expressed in an observation area is effectively observed. An observing apparatus includes a first observing unit performing a time lapse shooting of a predetermined observation area, a first discriminating unit discriminating whether or not a first material is expressed in the observation area based on an image obtained by the first observing unit, and a second observing unit starting a time lapse shooting relating to a part where the first material is expressed at a timing when the first material is expressed in the observation area, in which a shooting frequency of the time lapse shooting by the second observing unit is higher than a shooting frequency of the time lapse shooting by the first observing unit.

Abstract: Embodiments provide slide navigation technology that addresses challenges in digital pathology of navigating and viewing high resolution slide images. Example systems comprise a virtual slide stage (VSS) having at least one sensor that detects user movement of a target placed on the VSS, and an input component, coupled to the VSS, which provides quick function movement control of the target via quick functions. The systems also comprise a connector component that connects the VSS to a user device and transmits output from the at least one sensor and input component to the user device. The systems further comprise a computer processor, in communication with the VSS, which processes the output using a computational model to generate data representing movement profiles of the target. The computer processor executes a software component, causing the output, translated based on the movement profiles, to be relayed via a viewing application on the user device.

Abstract: A comparing method includes a processing trace forming step of positioning a condenser at a reference height and a plurality of heights by moving the condenser, and forming a plurality of processing traces in one surface of a workpiece by irradiating different positions of the one surface with a laser beam according to each of the heights, a calculating step of calculating at least one of an average of widths in a plurality of predetermined directions of each of the plurality of processing traces and an area ratio of each of the plurality of processing traces to a circle of a predetermined diameter by analyzing an image of the plurality of processing traces by an image analyzing section, and a comparing step of quantitatively comparing deviations of the plurality of processing traces from a predetermined shape on the basis of at least one of the average and the area ratio.

Abstract: A stage apparatus for a microscope includes a first stage provided on a mirror base of the microscope and fixed to a stage member that moves in an optical axis direction, a second stage that relatively moves over a surface of the first stage in a first direction, a third stage that relatively moves over a surface of the second stage in a second direction, the third stage having a placement portion for placing a microscope slide, and an exterior cover for covering at least a portion of the second stage and the third stage, the exterior cover being fixed to the first stage or the stage member. The exterior cover provides a space for the second stage and the third stage to move, and exposes the placement portion of the third stage.

Abstract: Apparatus and methods are described including acquiring microscopic images of a blood sample, and identifying deformed red blood cells within the microscopic images. A degree of red blood cell deformity within the sample is determined, at least partially based upon the identified deformed red blood cells. A sample-informative parameter that is indicative of a characteristic of the blood sample as a whole is determined, at least partially based upon the degree of red blood cell deformity within the sample. Other applications are also described.

Abstract: A dry objective includes: first lens group that includes a plurality of meniscus lens components and has a positive power; a second lens group that includes a cemented lens, turns a pencil of diverging light from the first lens group into a pencil of converging light, and has a positive power; and a third lens group that includes a front group and a rear group and has a negative power, the front and rear groups having concave surfaces adjacent to and facing each other. The dry objective satisfies the following conditional expression: 0.43?(hg2?hg1)/gt1?0.9??(1) where gt1 indicates a thickness that a lens component of the rear group that is the closest to the object has on an optical axis; hg1 and hg2, the heights of an axial marginal light ray at the lens surface of the lens component that is the closest to the object and an image.

Abstract: A method of determining a displacement comprises: generating an interferometric superoscillatory field from coherent electromagnetic radiation, the interferometric superoscillatory field comprising an interference pattern between a reference field and a superoscillatory field; detecting with a detector a first set of intensity distributions of the interferometric superoscillatory field, each intensity distribution from a different polarisation state of the electromagnetic radiation; detecting with the detector a second set of intensity distributions of the interferometric superoscillatory field, each intensity distribution from the same polarisation states of the electromagnetic radiation as the first set of intensity distributions; extracting a first local wavevector distribution from the first set of intensity distributions and a second local wavevector distribution from the second set of intensity distributions; comparing the first local wavevector distribution and the second local wavevector distribution

Abstract: A measurement chip including a prism, a metal film, and a capturing body is prepared. In a state in which a specimen is present on the metal film, scattered light obtained when first light which passes through the metal film and the specimen is scattered in the specimen when the first light is applied to the metal film from a prism side at a first incident angle smaller than a critical angle is detected. In a state in which a substance to be measured is captured by the capturing body and the specimen is not present on the metal film, a signal indicating an amount of the substance to be measured generated in the measurement chip when second light is applied to the metal film at a second incident angle not smaller than the critical angle from the prism side is detected. On the basis of a hematocrit value of the specimen determined from a light amount of the scattered light, a measurement value indicating the amount of the substance to be measured determined from the signal is corrected.

Abstract: The instant disclosure provides methods of extracting stain-independent features from digital images of histologically stained cells. Stain-independent features provide consistent assessment of cell morphology in the presence of staining variation and across different stains or stain formulations. Improved consistency in cell morphology assessments finds use in automated cell classification and other image processing applications. Also included are systems for practicing the described methods. The instant disclosure also provides computer readable media storing instructions that, when executed by a computing device, cause the computing device and/or components of a described system to perform steps of a method involving of extraction of stain-independent features from digital images.

Abstract: To enable evaluation of a shape of a fine particle and a fine particle type, a substrate is set as a substrate on which an isolated fine particle to be measured and an isolated standard fine particle in the vicinity of the isolated fine particle to be measured are disposed, and a scanning electron microscope body including a detector configured to detect secondary charged particles obtained by scanning a surface of the substrate with an electron beam probe, and a computer that processes a detection signal and generates an image of the isolated fine particle to be measured and the isolated standard fine particle are provided. The computer corrects a shape of the isolated fine particle to be measured by using a measurement result of the isolated standard fine particle disposed in the vicinity of the isolated fine particle to be measured.

Abstract: A method for counting blood cells in a sample of whole blood. The method comprises the steps of: (a) providing a sample of whole blood; (b) depositing the sample of whole blood onto a slide, e.g., a microscope slide; (c) employing a spreader to create a blood smear; (d) allowing the blood smear to dry on the slide; (e) measuring absorption or reflectance of light attributable to the hemoglobin in the red blood cells in the blood smear on the slide; (f) recording a magnified two-dimensional digital image of the area of analysis identified by the measurement in step (e) as being of suitable thickness for analysis; and (g) collecting, analyzing, and storing data from the magnified two-dimensional digital image. Optionally, steps of fixing and staining of blood cells on the slide can be employed in the method.

Abstract: An image pickup apparatus includes an image pickup device, a display device including, in a finder loupe visual field, an image display region and an image display outside region, and a display controller configured to control the display device. The display controller determines a difficulty degree of visibility of a boundary between the image display region and the image display outside region and controls the display device to draw a boundary display when the difficulty degree is equal to or larger than a predetermined value and not to draw the boundary display when the difficulty degree is smaller than the predetermined value.