Abstract: Enhancement of fluorescence emission from fluorophores bound to a sample and present on the surface of two-dimensional photonic crystals is described. The enhancement of fluorescence is achieved by the combination of high intensity near-fields and strong coherent scattering effects, attributed to leaky photonic crystal eigenmodes (resonance modes). The photonic crystal simultaneously exhibits resonance modes which overlap both the absorption and emission wavelengths of the fluorophore. A significant enhancement in fluorescence intensity from the fluorophores on the photonic crystal surface is demonstrated.

Abstract: A method of producing spatial fine structures comprises the steps of: selecting a luminophore from the group of luminophores displaying two different states, one of the two states being an active state in which luminescence light is obtainable from the luminophore, the other of the two states being an inactive state in which no luminescence light is obtainable from the luminophore, and the luminophore being reversibly, but essentially completely, transferable out the one state into the other state by means of an optical signal; adding the luminophore to a material; forming a spatial fine structure of the material; and fluorescence-microscopically examining whether the desired fine structure is present.

Abstract: A method for measuring the presence or concentration of an analyte in a sample by spectrophotometry: providing an open top cuvette having a sample with an analyte to be measured; providing a light source and a detector for detecting emitted light; taking at least two measurements that includes: (i) directing at least two beams of light from the light source to different locations on the cuvette; (ii) passing the at least two beams through the cuvette at their respective locations and through the sample to be measured; and (iii) measuring at least two respective emitted light beams with the detector; and comparing the at least two emitted light beams to determine if: all the emitted light beams should be disregarded; one or more of the emitted light beams should be disregarded; or the sample absorbances should be averaged. In a preferred embodiment, the method includes taking at least three measurements.

Abstract: A fluorescent detection apparatus relates to an analysis technique for qualitatively detecting or quantifying biomolecules by producing an evanescent field on a surface of a substrate, exciting fluorescently labelled biomolecules on the substrate surface in the evanescent field, and detecting the resultant fluorescent light emitted from the biomolecules. The fluorescent detection apparatus has a configuration in which a well is provided in a surface opposing to a sample substrate of a prism, the well is filled with a matching liquid, and the matching liquid is filled between the sample substrate and the prism, thereby improving operability and providing a stable evanescent field.

Abstract: A time-resolved spectroscopy system employing a time-division multiplexing optical device with no dispersive optical elements to perform lifetime and concentration measurements in multi-species samples, is disclosed. Some examples include fluorescence and cavity ring-down spectroscopy. The system is unique in its compactness and simplicity of operation. In one embodiment, the system makes use of only one photo-detector and an efficient linear regression algorithm. The system offers a measurement time for multiple species measurements of less than 1 s. The system can also be used to perform fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy. Four methods to de-convolve a multi-component, exponentially decaying optical signal such as obtained with the system disclosed here, are presented.

Abstract: An apparatus for estimating a property of a fluid in an earth formation, the apparatus including: a logging instrument configured to be conveyed in a borehole penetrating the formation; and a plurality of light sources disposed at the logging instrument; wherein each of the light sources is configured to illuminate a sample of the fluid with a light beam causing the sample to fluoresce light with a characteristic related to the property, each of the light sources being configured to provide a light beam with a solid angle and a distance traveled to the sample, the solid angle and the distance being configured to concentrate the beam at an area of the sample that is overlapped substantially a same amount by a beam from another light source in the plurality.

Abstract: A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.

Abstract: An object authentication system for authenticating an object includes a taggant material applied to the object; a database storing data related to the excitation-emission properties of the taggant material and to an identity of the object; and an authentication reader. The authentication reader includes an excitation source for emitting light for exciting the taggant material, an emission detection device for detecting light emission of the excited taggant material, and a processing unit for analyzing the detected light emission, comparing the detected light emission profile with data stored in the database, and thereby verifying the identity of the object. The taggant material includes a fluorescent material and the taggant material is applied to the object by being mixed into the raw material of the object, being integrated with a portion of the object, or being attached to the object by an adhesive material. A method for authenticating an object is also provided.

Abstract: An active CMOS biosensor chip for fluorescent-based detection is provided that enables time-gated, time-resolved fluorescence spectroscopy. In one embodiment, analytes are loaded with fluorophores that are bound to probe molecules immobilized on the surface of the chip. Photodiodes and other circuitry in the chip are used to measure the fluorescent intensity of the fluorophore at different times. These measurements are then averaged to generate a representation of the transient fluorescent decay response unique to the fluorophores. In addition to its low-cost, compact form, the biosensor chip provides capabilities beyond those of macroscopic instrumentation by enabling time-gated operation for background rejection, easing requirements on optical filters, and by characterizing fluorescence lifetime, allowing for a more detailed characterization of fluorophore labels and their environment.

Abstract: The invention relates to a spectrometer for analysing the optical emission of a sample, having an excitation source, an entrance gap and a dispersive element, which fans out the spectrum of the light generated in the excitation source in a plane, and having solid body sensors with one or more lines, which are arranged in the region of the focal curve of the beam path in order to evaluate the spectral information, wherein the sensors are arranged above or below the plane and the spectral emission is deflected onto the sensors by mirrors and focused, wherein the reflecting surface of the mirrors is aspherically formed in a direction of curvature.

Abstract: An on-chip micro-fluidic device (10) fabricated using a semiconductor material. The device has a micro-fluidic channel or chamber (14) defined within the material and one or more monolithically integrated semiconductor lasers (12) operate to form an optical trap in the channel or chamber (14).

Abstract: A camera system uses a highly sensitive camera such as an intensified charge-coupled-device camera to acquire images. An image acquisition and processing tool can place the camera in a low-sensitivity mode and a high-sensitivity mode. In the low-sensitivity mode, a reference image may be acquired of a target that is illuminated by a light-emitting-diode. In the high sensitivity mode, low-photon-flux image data frames are acquired using a charge-coupled-device image sensor in the camera. The image acquisition and processing tool displays the acquired image data frames on top of the reference image in real time, so that a user is provided with immediate visual feedback. The image acquisition and processing tool has image preprocessing filters for enhancing image quality such as a sensor noise threshold filter, a cosmic ray filter, and a photon shape optimization filter. Pipeline filters may be used to further process acquired image data frames.

Abstract: An improvement in a method for quantitative modulated imaging to perform depth sectioned reflectance or transmission imaging in a turbid medium, such as human or animal tissue is directed to the steps of encoding periodic pattern of illumination preferably with a fluorescent excitation wavelength when exposing a turbid medium to the periodic pattern to provide depth-resolved discrimination of structures within the turbid medium; and reconstructing a non-contact three dimensional image of the structure within a turbid medium. As a result, wide field imaging, separation of the average background optical properties from the heterogeneity components from a single image, separation of superficial features from deep features based on selection of spatial frequency of illumination, or qualitative and quantitative structure, function and composition information is extracted from spatially encoded data.

Abstract: Apparatus and method for measuring the fluorescence of nanodrop liquid samples is described in which the sample is held by surfa tension between two anvil surfaces (20, 24). Each anvil surface (20, 24) has an embedded optical fiber (18) with its end finished fl with the surface in the containment area wetted by the sample with the fiber (18) in line. Sample excitation is provided from the sid of the sample remote from the containment area. By selection of the fiber transmission numeric aperture the impact of exciting and ambient light on the measurement is minimized. A method of virtual filtering is taught in which any ambient or exciting light that does impinge on the measuring sensor is corrected by subtracting a scaled representation of the source from the measurement. The method and apparatus is capable of detecting (1) femptomole of sodium fluorescein in (1) microliter of TE buffer.

Abstract: Many points on a sample are tested simultaneously in parallel in an FCS method with multifocal illumination and/or detection.

Abstract: A small object identifying device and its identifying method according to which a large number of small objects can be identified. In one embodiment, the device includes a dispersion region section which disperses a large quantity of several kinds of small objects which are labeled by a combination of the presence/absence or measure of label elements of several kinds. A measuring device distributes and associates kinds of said label elements to two or more measurement points and measures the presence/absence or the measure of said label elements of the kinds which have been associated with respective measurement points. An identifying section associates the measurement results measured at each measurement point to thereby identify said small objects.

Abstract: A method of imaging with an optical system characterized by a diffraction-limited resolution volume is disclosed. In a sample that includes a plurality of phototransformable optical labels (“PTOLs”) distributed in the sample with a density greater than an inverse of the diffraction-limited resolution volume of the optical system, a first subset of the PTOLs in the sample are activated, and the density of the activated PTOLs in the first subset is less than the inverse of the diffraction-limited resolution volume. A first portion of the PTOLs in the first subset of PTOLs is excited. Radiation emitted from the activated and excited PTOLs in the first portion of PTOLs is detected with the imaging optics, and locations of activated and excited PTOLs in the first portion of PTOLs is determined with a sub-diffraction-limited accuracy based on the detected radiation emitted from the activated and excited PTOLs.

Abstract: This relates to methods and apparatus for analyzing samples by fluorescence spectroscopy. In one embodiment, the methods and apparatus use a fluorescence detection unit (FDU) of a composition fluid analyzer (CFA™) module to detect variations in fluid properties (gradients) within an oil bearing column. Some embodiments enable efficient downhole fluid analysis in heavy oils where water/oil emulsions are present (water in dispersed phase and oil in the continuous phase). The principles described herein may also be applied when there are fine particles in the oil such as from unconsolidated sands.

Abstract: The system and method of the present invention relate to characterizing lipoproteins in a sample. The system includes a light source that delivers electromagnetic energy in a predetermined range of wavelengths to the sample and a sensor that senses an intensity spectrum which emerges from the sample when the sample is illuminated by the light source. A processor determines a chemical composition of the sample to determine the presence of lipoprotein particles. The processor then characterizes lipoproteins that are within in the sample by deconvoluting the intensity spectrum into a scattering spectrum and absorption spectrum. The method includes illuminating the sample with electromagnetic energy having a predetermined range of wavelengths and sensing the electromagnetic energy that emerges from the sample. The method further includes transducing the sensed electromagnetic energy which emerges from the sample into an intensity spectrum that determines the types of lipoproteins that are within the sample.

Abstract: Methods and apparati for fluorescence imaging using multiple excitation-emission pairs is revealed. A target object is illuminated by light in at least two spectral regions, causing fluorescence emission in at least two spectral regions. The emitted light is collected and separated for analysis.

Abstract: A system and method of detecting explosive compounds located on a sample. The sample is irradiated with animal-safe ultra-violet radiation generating a fluorescence data set. A fluorescence database is searched based on the fluorescence data set in order to identify a known fluorescence data set. If the searching of the fluorescence database identifies a known fluorescence data set, an area of interest in the sample is identified based on the known fluorescence data set identified in the fluorescence database searching. The area of interest is irradiated with substantially monochromatic radiation to generate a Raman data set of the area of interest. A Raman database is searched based on the Raman data set in order to identify a known Raman data set. An explosive compound in the area of interest is identified based on the known Raman data set identified by searching the Raman database.

Abstract: A system and method for standoff detection of explosives and explosive residue. A laser light source illuminates a target area having an unknown sample producing luminescence emitted photons, scattered photons and plasma emitted photons. A first optical system directs light to the target area. A video capture device outputs a dynamic image of the target area. A second optical system collects photons, and directs collected photons to a first two-dimensional array of detection elements and/or to a fiber array spectral translator device which device includes a two-dimensional array of optical fibers drawn into a one-dimensional fiber stack. A spectrograph is coupled to the one-dimensional fiber stack of the fiber array spectral translator device, wherein the entrance slit of the spectrograph is coupled to the one dimensional fiber stack.

Abstract: With the measurement unit (7) empty and with the maximum (or approximately 100%) transmissivity of the first dimmer apparatus (3) and the second dimmer apparatus (4), the output voltages of the preamplifier (11) (i.e. the value Pa corresponding to the first light source 1 and the value Pb corresponding to the second light source 2) are stored in the memory unit (16). The appropriate transmissivity computation unit (17) compares the Pa value with the Pb values, sets the smaller value as Ps, and computes the appropriate transmissivity (Ps/Pa)×100 of the dimmer apparatus (3) and the appropriate transmissivity (Ps/Pb)×100 of the dimmer apparatus (4). The controller (15) sets the transmissivities of the dimmer apparatus (3) and the dimmer apparatus (4) to have the previously obtained respective appropriate transmissivities. As a result, both the output voltages (or the values corresponding to the first light source (1) and second light source (2)) of the preamplifier (11) become Ps.

Abstract: Emitting illumination light and auxiliary light having a wavelength range different from that of the illumination light onto an observation target simultaneously, obtaining an image formed of reflection light of the illumination light and reflection light of the auxiliary light reflected from the observation target, calculating, with respect to each pixel of the obtained image signal, estimated spectroscopic data in the wavelength range of the auxiliary light using a value of the image signal and estimated matrix data in the wavelength range of the auxiliary light stored in advance, obtaining quasi reflectivity information reflecting a reflectivity of the observation target in the wavelength range of the auxiliary light based on the estimated spectroscopic data in the wavelength range of the auxiliary light, and generating a special image based on the quasi reflectivity information.

Abstract: The present invention discloses a coupled waveguide-surface plasmon resonance biosensor, comprising: a grating layer formed of a transparent material, the grating layer comprising a first periodic grating structure; a waveguide layer formed on the first periodic grating structure, the refractive index of the waveguide layer being larger than the refractive index of the grating layer; a plasmon resonance layer formed on the waveguide layer, capable of being optically excited to cause a plasmon resonance wave; and a ligand layer formed on the plasmon resonance layer; capable of being bound to react with receptors of a sample to be tested.

Abstract: The present invention is directed to a novel multi-spectral exogenous fluorescence polarization imaging technique that enables rapid imaging of large tissue fields. The imaging device includes a tunable monochromatic light source and a CCD camera. Linear polarizers are placed into both the incident and collected light pathways in order to obtain fluorescence polarization or/and anisotropy image. To acquire exogenous fluorescence image, fluorescent contrast agents are delivered to a target tissue.

Abstract: The invention concerns a method for automatically differentiating between a sample liquid and a control liquid especially within the context of analytical measuring systems, wherein the presence of a special property of the control liquid and/or at least two criteria are used for the differentiation. In addition the invention concerns appropriate control liquids that are suitable for the new method.

Abstract: An autofocus mechanism for a spectroscopic system determines a time varying optical property of a volume of interest. The mechanism measures the fluctuations of the optical property of the volume of interest for determining the position of the volume of interest. The spectroscopic system focuses an excitation beam into the determined volume of interest and collects return radiation emanating from the volume of interest for spectroscopic analysis. Preferably, inelastically scattered radiation of an excitation beam is separated from elastically scattered radiation for spectroscopic analysis. The elastically scattered radiation of the excitation beam is measured for fluctuations of the optical property of the volume of interest. A control loop maximizes the amplitude and/or intensity of the fluctuations and specifies the position of a volume of interest e.g. the center of a capillary vessel.

Abstract: The invention relates to a method and device for analyzing a tissue (70), which comprises: —irradiating the tissue (70) with light focused on a focal region (40); —collecting light coming back from the focal region (40) into a first detection device (100A), the first detection device (100A) being arranged to only collect the light coming back from the focal region (40), on a first detection area (140A), by confocal spectroscopy, in order to generate a first signal, containing information on an optical property of the tissue (70); —collecting light, scattered from the focal region (40) to at least a second region (60), coming back from the second region (60), into a second detection device (100B), the second detection device (100B) being arranged to only collect the light coming back from the second region (60), on a second detection area (140B), in order to generate a second signal, —using the first and second signals in order to get information on the scattering and/or absorption coefficients of the tissue (

Abstract: A biochip reader wherein spectroscopic information of a sample under analysis is arranged in spaces between images of the sample arranged on a biochip. The reader comprises a confocal microscope and the biochip comprises a transparent substrate to allow passage of the excitation light and fluorescent light from the sample with the excitation light being applied from the side opposite that on which the samples are arranged so that noise from dust and the like is avoided by the transmitted light avoiding contact with the dust. Another aspect is an electrophoresis system wherein different coloring material are used for each of a variety of target substances, so that the same lane and area are utilizable to concurrently detect a polychrome fluorescent pattern of the different targets. A confocal scanner or fluorescence imaging system is used with a plurality of filters to detect the multi-colored fluorescences of the target substance.

Abstract: A spectroscopic analysis method in which spectral data of mixtures obtained from a plurality of points on a sample surface are resolved into component spectra and concentrations. A new alternating least squares multivariate curve resolution technique is presented which iteratively resolves the components. The technique starts from an initial estimate that the spectral values of a first component of the sample are all equal (an ‘empty model’), and resolves that component. Then successive further components are iteratively resolved, from initial ‘empty model’ estimates of those components and from previously resolved spectra. In the common case where the main component is present in nearly pure form in the data set, this empty modelling technique results in more accurate resolution of the components. This is due to the ability of the technique to resolve the pure spectra of minor components without modelling concentrations of the main component into them.

Abstract: The invention relates to a device and method for optical examination and for evaluation of a biological active and/or biological activatable substance (2) by use of infra-red, visible or ultra-violet light, at which the examined, respectively evaluated substance is optically stimulated, so that the substance either emits light (in particular fluorescence analysis), or modifies the wavelength of the used light (in particular raman analysis), whereas the device comprises: An electrically driven light source (3), an optical light guide (4), by which the light emitted by the light source (3), is transferable as radiation to the substance being examined (2), an optical or opto-electronical sensor device (5) dedicated to the substance being examined (2), to receive the light emitted or modified by the biological active and/or biological activatable substance, an optical or opto-electronical spectrometer device (6) dedicated to the sensor device (5), by which the light emitted or modified by the substance (2) is

Abstract: Portable, field-deployable laser synthesizer devices designed for multi-dimensional spectrometry and time-resolved and/or hyperspectral imaging include a coherent light source which simultaneously produces a very broad, energetic, discrete spectrum spanning through or within the ultraviolet, visible, and near infrared wavelengths. The light output is spectrally resolved and each wavelength is delayed with respect to each other. A probe enables light delivery to a target. For multidimensional spectroscopy applications, the probe can collect the resulting emission and deliver this radiation to a time gated spectrometer for temporal and spectral analysis.

Abstract: The method enables a heterogeneous object containing fluorophores to be examined. A first face of the object is illuminated with an excitation light exciting the fluorophores. The light emitted by a second face of the object, opposite the first face, is detected by means of a matrix of detectors. The fluorophore distribution is determined by means of relevant Green's functions each associated with a selected source and/or detector, able to be assimilated to a point of the surface of the object. Thus, a first spatial coordinate of each of the relevant Green's functions corresponds to a point of the first face of the object and/or a second spatial coordinate of each of the relevant Green's functions corresponds to a point of the second face of the object.

Abstract: An IR spectroscopic method of determining the presence or absence of metallic material embedded in polymer material including irradiating a surface of said polymer material and collecting at least one spectrum of reflected infrared energy from said surface of said over a spectrum of wavelengths including the near-infrared wherein said metallic material is at least partially absent in a affected condition; performing a multivariate comparison of said at least one spectrum with at least one reference spectrum to determine a change in reflectance of said at least one spectrum with respect to at least one reference spectrum wherein said metallic material is present in an unaffected condition; and, correlating the amount of said change in reflectance with the presence or absence of said metallic material embedded in said polymer material.

Abstract: The invention provides an apparatus including (a) a frame having a boundary plane; (b) a flow chamber supported by the frame, the flow chamber placed a distance from the boundary plane; (c) a radiation source, the radiation source directed away from the flow chamber and away from the exterior side of the boundary plane, and (d) a first reflective surface placed to direct a radiation beam in a path crossing the boundary plane to the flow chamber; (e) one or more reflective surfaces placed to direct a radiation beam from the radiation source to the first reflective surface, the path from the radiation source to the flow chamber being at least 1.5 times the distance from the flow chamber to the boundary plane.

Abstract: A microscope for optical imaging of high optical scattering coefficient biological tissue, comprising an optical excitation source for irradiating a scan area of the sample and generating optical emissions, wherein the sample has a first face facing away from the source and a second face facing the source. A two dimensional element for scanning the light over the sample; a focusing element having a numerical aperture NAi to focus the light onto the sample; a first optical condenser to collect light from the first face, the collected light comprising source transmitted light and first optical emission generated in the sample, the condenser having a NA2 larger than NAi; an optical filter to block the transmitted source light; an aperture with a size corresponding to the irradiated area of the sample, the aperture at the conjugate image position of the sample generated by the condenser; and an optical detector collecting light from the first face for detecting the first optical emission from the scan area.

Abstract: Disclosed herein is a spectrophotometer. The spectrophotometer includes a CPU having a signal prediction part and a comparison/calculation part. The signal prediction part predicts the strength of an output signal from a photodetection unit during the next period based on the strength of the output signal from the photodetection unit. The comparison/calculation part compares a reference value, which defines the limit value of electrical current passing through a photomultiplier tube, of the strength of an output signal from an AD converter with a predicted value predicted by the signal prediction part. In a case where the predicted value exceeds the reference value, a voltage applied to the photomultiplier tube is calculated so that the strength of an output signal from the photodetection unit during the next period does not exceed the reference value.

Abstract: System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.

Abstract: A method for determining the degree of mixing between components in a mixing process, the method including the steps of: a) mixing at least two components and at least two luminescent materials to form a mixture, wherein the luminescent materials are added to the mixture separately from each other, and wherein each luminescent material has a uniquely detectable luminescence emission wavelength; b) detecting emitted luminescence from a sample of the mixture, wherein the emitted luminescence includes different luminescence intensities at the uniquely detectable luminescence emission wavelengths of the luminescent materials; c) wherein the ratio of luminescence intensities and/or the absolute or relative intensities of luminescence at the uniquely detectable luminescence emission wavelengths is indicative of the degree of mixing between the components.

Abstract: A method and a microscope, in particular a laser scanning fluorescence microscope, for high spatial resolution examination of samples, the sample (1) to be examined comprising a substance that can be repeatedly converted from a first state (Z1, A) into a second state (Z2, B), the first and the second states (Z1, A; Z2, B) differing from one another in at least one optical property, comprising the steps that the substance in a sample region (P) to be recorded is firstly brought into the first state (Z1, A), and that the second state (Z2, B) is induced by means of an optical signal (4), spatially delimited subregions being specifically excluded within the sample region (P) to be recorded, are defined in that the optical signal (4) is provided in such a way that a standing wave with defined intensity zero points (5) is formed in the sample region (P) to be recorded.

Abstract: Methods and systems for real-time monitoring of optical signals from arrays of signal sources, and particularly optical signal sources that have spectrally different signal components. Systems include signal source arrays in optical communication with optical trains that direct excitation radiation to and emitted signals from such arrays and image the signals onto detector arrays, from which such signals may be subjected to additional processing.

Abstract: A gamma ray detector assembly for a borehole logging system that requires the measure of gamma radiation with optimized gamma ray energy resolution and with fast emission times required to obtain meaningful measurements in high radiation fields. The detector assembly comprises a lanthanum bromide (LaBr3) scintillation crystal and a digital spectrometer that cooperates with the crystal to maximize pulse processing throughput by digital filtering and digital pile-up inspection of the pulses. The detector assembly is capable of digital pulse measurement and digital pile-up inspection with dead-time less than 600 nanoseconds per event. Pulse height can be accurately measured (corrected for pile-up effects) for 2 pulses separated by as little as 150 nanoseconds. Although the invention is applicable to virtually any borehole logging methodology that uses the measure of gamma radiation in harsh borehole conditions, the invention is particularly applicable to carbon/oxygen logging.

Abstract: A system and method to obtain a variable field of view (FOV) of a sample without requiring an increase in an imaging CCD array size. In a fiber array spectral translator (FAST) based chemical imaging system, the fibers in the fiber bundle may be organized in different 2D “zones”. Each zone may include a predetermined number of fibers. Each 2D zone of fibers at the signal input end is organized as a separate linear array (1D) at the spectrometer slit input end. Depending on the user-selected FOV, one or more zones of fibers may be selected for signal input (into the spectrometer) by a motorized mobile slit port or linear translating stage, which will sequentially scan output from each selected linear fiber array into the spectrometer slit. The user can switch from one FOV size to another, thereby activating the linear translating stage to gather signals from appropriate linear fiber arrays corresponding to fiber zones associated with the selected FOV.

Abstract: An apparatus includes a position-sensitive detector to detect intensities of radiation as a function of position on the detector, and an optical system, characterized by a diffraction-limited resolution volume, adapted for imaging light emitted from activated and excited phototransformable optical labels (“PTOLs”) in a sample onto the position sensitive-detector. A first light source provides activation radiation to the sample to activate a subset of the PTOLs that are distributed in the sample with a density greater than an inverse of the diffraction-limited resolution volume of the optical system. A second light source provides excitation radiation to the sample to excite a portion of the PTOLs in the subset of the PTOLs. A controller controls one both of the activation radiation and the excitation radiation provided to the sample such that a density of PTOLs in the portion of the PTOLs is less than the inverse of the diffraction-limited resolution volume.

Abstract: First activation radiation is provided to a sample that includes fluorescent proteins (“FPs”) to activate a first subset of the FPs in the sample. First excitation radiation is provided to the first subset of FPs in the sample to excite at least some of the activated FPs, and radiation emitted from activated and excited FPs within the first subset of FPs is detecting with imaging optics. The first activation radiation is controlled such that the mean volume per activated FPs in the first subset is greater than or approximately equal to a diffraction-limited resolution volume (“DLRV”) of the imaging optics.

Abstract: The present invention relates to an optical manipulation system (10) for generation of a plurality of optical traps for manipulation of micro-objects including nano-objects using electromagnetic radiation forces in a micro-object manipulation volume (14), the system comprising a spatially modulated light source (16) for emission of a first light beam (24) with a spatially an adjustable spatial light modulator (26) for generating a second (28) and a third (30) modulated light beam, the second intensity modulated light beam propagating towards a first deflector (32) for deflection of the second light beam towards the micro-object manipulation volume (14), and the third intensity modulated light beam propagating towards a second deflector (34) for deflection of the third light beam towards the same volume (14).

Abstract: Instrumentation for measuring luminescence phase lag to quantitate an analyte concentration is corrected to eliminate or reduce extraneous phase lag noise. A calibration factor is determined in steps that are interspersed between quantitative measurements. An optical pathway is provided to accomplish the calibration by the provision of a second optical source that emits in the luminescence emission band of a luminescent material. The calibration factor may be subtracted from measurement of the quantification phase lag to correct for extraneous phase lag.

Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.

Abstract: The present invention relates to equipment for measuring water quality using a fish or a water flea, an more particularly, to equipment for measuring water quality in which small organisms such as killifish (or medaka) or water flea are put into test chamber having a plurality of divided sections and behavior of the fishes or the water fleas accommodated in the test chamber is observed while continuously flowing water of a river or a water zone to be examined through the test chamber, thereby finding out and measuring existence of toxic element or harmful substance included in the water to be examined.