With Light Detector Patents (Class 356/442)
  • Patent number: 7365852
    Abstract: Methods and sub-systems for substantially optimizing the absorbance measurement in optical instruments are provided. A method comprises forming a liquid sample into a droplet extending between two opposing surfaces, passing a light beam through the sample, and varying the distance between the two opposing surfaces until a distance substantially corresponding to a optimum absorbance is obtained.
    Type: Grant
    Filed: November 24, 2004
    Date of Patent: April 29, 2008
    Assignee: Agilent Technologies, Inc.
    Inventor: Arthur Schleifer
  • Patent number: 7362429
    Abstract: The present invention is directed to the use of a light absorbing wall material to eliminate stray light paths in light-guiding structures, such as those used for HPLC absorbance detection. More specifically, the present invention relates to the use of carbon-doped Teflon® AF, or “black Teflon® AF,” for all or part of the walls of a light-guiding flowcell adapted for use in HPLC absorbance detection.
    Type: Grant
    Filed: August 4, 2004
    Date of Patent: April 22, 2008
    Assignee: Waters Investments Limited
    Inventor: Anthony C. Gilby
  • Patent number: 7342660
    Abstract: An apparatus comprises a detector, a pressure sensor and a processor. The detector is operable to detect light that is scattered by an aerosol that is associated with a pressure. The pressure sensor is operable to measure the pressure. The processor is coupled to the detector and to the pressure sensor, and is configured to receive at least a signal from the detector and the pressure sensor. The processor is further configured to use the received signals to calculate a volume of the first aerosol, and to output an output signal associated with the calculated volume. The various measurements can be repeated and compared, and the output signal can be a feedback signal for metering subsequent amounts of the aerosol, based on the comparison.
    Type: Grant
    Filed: September 30, 2003
    Date of Patent: March 11, 2008
    Assignee: DEKA Products Limited Partnership
    Inventors: David E. Altobelli, Larry B. Gray, Derek G. Kane
  • Patent number: 7333206
    Abstract: In accordance with an embodiment of the present invention, an apparatus for measuring light scatter loss from a test piece includes a light source, an optical cavity including the test piece and at least one mirror located along a path, and a light detector. The cavity receives an input beam from the light source, circulates a beam within the cavity as a circulating beam, and produces an output beam. Irregularities on the surface of the test piece result in a progressive diffusion of the circulating beam about the path. The light detector provides an output signal based on the intensity of the output beam. The output signal has an initial slope that determines a first saturation value. The output signal has a second saturation value. The difference between the first saturation value and the second saturation value provides a scatter loss measurement.
    Type: Grant
    Filed: April 7, 2005
    Date of Patent: February 19, 2008
    Assignee: The Bowing Company
    Inventor: Roy Clark
  • Patent number: 7271381
    Abstract: The invention relates to a device to detect individual moving objects having very small dimensions.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: September 18, 2007
    Assignee: Minebea Co., Ltd.
    Inventors: Martin Arnold, Norbert Irmer, Hubert Schill, Markus Heinzler
  • Patent number: 7201875
    Abstract: A flow cell and flow cytometer in which a nozzle at the end of a flow channel is disposed on a removable substrate held at a registered location on a flow cell. Other elements including illumination optics, light collection optics, and the flow cell may then be positioned at fixed locations and would not require subsequent periodic adjustment. The registered location for positioning the nozzle allows removal and replacement of the nozzle key with the nozzle subsequently positioned in the identical location.
    Type: Grant
    Filed: September 27, 2002
    Date of Patent: April 10, 2007
    Assignee: Becton Dickinson and Company
    Inventors: Pierce O. Norton, David R. Vrane, Shervin Javadi
  • Patent number: 7149396
    Abstract: An optical waveguide is constructed so as to comprise a non-solid core layer surrounded by a solid-state material. The non-solid core layer has an index of refraction which is lower than the index of refraction of the surrounding solid-state material, and light can be transmitted with low loss through the non-solid core layer. In an exemplary application, the non-solid core layer comprises a sample material whose light transmission, absorption, and/or interference characteristics are to be measured. In addition, a perpendicular waveguide portion may be included for use in injecting light into the core for measuring fluorescence characteristics associated with the sample material. Most preferably, the optical waveguide is generally structured as an anti-resonant reflecting optical waveguide (ARROW), which comprises a Fabry-Perot reflector adjacent to the core layer, whereby light is substantially prevented from leaking out of said core in a transverse direction.
    Type: Grant
    Filed: June 15, 2004
    Date of Patent: December 12, 2006
    Assignees: The Regents of the University of California, Brigham Young University
    Inventors: Holger Schmidt, Aaron Roe Hawkins
  • Patent number: 7145165
    Abstract: A sensitive fluid sensor for detecting fluids and particularly trace fluids. The sensor may be adjustable for detecting fluids of various absorption lines. To effect such adjustment, a tunable laser may be used. The laser may be an edge emitting diode, a VCSEL or other tunable source. The detection apparatus of the sensor may incorporate a sample cell through which a laser light may go through. The sample cell may comprise a tunable ring-down cavity. The ring-down cavity may be a ring laser cavity like that of a ring laser gyroscope. There may be a photo detector proximate to the ring down cavity connected to a processor.
    Type: Grant
    Filed: September 28, 2004
    Date of Patent: December 5, 2006
    Assignee: Honeywell International Inc.
    Inventors: James A. Cox, Barrett E. Cole
  • Patent number: 7142305
    Abstract: There is provided a microchemical system which dispenses with the optical axis alignment and positioning of the focal point of the detecting light and that of the exciting light, and is capable of carrying out measurement with high sensitivity. The microchemical system includes a channel-formed plate-shaped member containing a sample, an exciting light source that irradiates exciting light onto the sample via a converging lens, a detecting light source that irradiates detecting light onto a thermal lens formed within the sample by the irradiated exciting light, via the converging lens, and detecting means for detecting the irradiated detecting light via the formed thermal lens. The exciting light source and the detecting light source are connected to the converging lens via an optical fiber for propagating the exciting light and the detecting light to the converging lens.
    Type: Grant
    Filed: March 25, 2005
    Date of Patent: November 28, 2006
    Assignee: Nippon Sheet Glass Company, Limited
    Inventors: Jun Yamaguchi, Akihiko Hattori
  • Patent number: 7142307
    Abstract: Apparatus and a method are disclosed for the simultaneous or rapid sequential use of two or more different separations between the source and detector of the measuring apparatus to obtain spectral measurement data in diffuse transmission or “interaction” modes of collecting optical information from a specimen. The method and apparatus subsequently combine separate data taken from two or more different pathlengths to provide discrimination against undesired information while preserving or enhancing desired information. Additional reference information to normalize the optical signal is also provided. The optical and mechanical design of the optical probe also provides for transmittance, reflectance and interactance measurements on small amounts of specimen.
    Type: Grant
    Filed: March 14, 1997
    Date of Patent: November 28, 2006
    Inventor: Edward W. Stark
  • Patent number: 7090808
    Abstract: Apparatus and method for testing one or more sterilization indicators (e.g., biological indicators or chemical indicators) or other articles by contacting them under controlled sterilization conditions with a flowing antimicrobial gas containing hydrogen peroxide vapor are disclosed. This invention also provides apparatus and method for testing sterilization processes that use a flowing antimicrobial gas containing hydrogen peroxide vapor under controlled sterilization conditions, or for testing materials for such processes under controlled sterilization conditions, or for testing both such processes and such materials under controlled sterilization conditions. There can be an essentially square-wave contact of the sterilization indicators or other articles with the antimicrobial gas (i.e.
    Type: Grant
    Filed: July 9, 2001
    Date of Patent: August 15, 2006
    Assignee: Pharmaceutical Systems, Inc.
    Inventors: Ross A. Caputo, Robert R. Reich, Jr., Robert J. Thrash, Jimmy Fisher, Davoud Khorzad, Thomas F. Cullen
  • Patent number: 7079315
    Abstract: A small and portable microscope for ultramicroanalysis has an excellent spatial resolution and fixed position analyzing ability. The microscope has at least a part of an excitation light source, a part of a probe light source and a part of a thermal lens microscope optical system arranged integrally in a device body. The device body also includes an analyzing cell and substrates supporting the analyzing cell.
    Type: Grant
    Filed: May 2, 2005
    Date of Patent: July 18, 2006
    Assignee: Kanagawa Academy of Science and Technology
    Inventors: Takehiko Kitamori, Akihide Hibara, Manabu Tokeshi
  • Patent number: 7057729
    Abstract: There is provided that enables high-sensitivity analysis to be carried out, and a photothermal conversion spectroscopic analysis apparatus that is compact in size and is able to implement the photothermal conversion spectroscopic analysis method. Exciting light and detecting light are convergently irradiated onto the sample by a converging lens. The sample is analyzed using a change in intensity of the detecting light due to refraction upon passing through the thermal lens produced through the convergent irradiation of the exciting light. A molar absorption coefficient of the sample at a wavelength of the exciting light is a predetermined number of times greater than a molar absorption coefficient of the sample at a wavelength of the detecting light.
    Type: Grant
    Filed: May 27, 2003
    Date of Patent: June 6, 2006
    Assignee: Nippon Sheet Glass Co., Ltd.
    Inventors: Jun Yamaguchi, Akihiko Hattori
  • Patent number: 6998021
    Abstract: In a paper machine which comprises process elements and a measuring beam, the measuring beam is arranged at least at one paper machine process element so that a measuring device for measuring quality properties of a paper web is arrangeable in the measuring beam. The paper machine process element is e.g. a blow box, which is arrangeable in a dryer unit of the paper machine between drying cylinders to blow air via at least one blow air channel provided in the blow box to guide the moving paper web and/or to assist drying.
    Type: Grant
    Filed: March 22, 2002
    Date of Patent: February 14, 2006
    Assignee: Metso Automation Oy
    Inventors: Markku Mäntylä, Matti Kukkurainen, Jari Koivu
  • Patent number: 6970248
    Abstract: A method for detecting a characteristic of a flow of a liquid in a pipe for intended use in determining a transition among or between two products forming the flow is disclosed. The method may comprise obtaining a first signal representative of the characteristic of the flow, determining when a substantial change in the characteristic is occurring; determining the transition point, and determining a transition time for the substantial completion of the transition based on parameter(s) generated from the first signal.
    Type: Grant
    Filed: May 17, 2003
    Date of Patent: November 29, 2005
    Assignee: Reflectronics, Incorporated
    Inventors: Fred A. Payne, Mary-Grace D. Danao
  • Patent number: 6930778
    Abstract: A microchemical system is provided, which can improve the working efficiency of the user and can be designed compact in size. The microchemical system is provided with an optical fiber 10 with a gradient index rod lens 102 mounted on an end thereof. The lens-possessing optical fiber 10 is comprised of an optical fiber 101 that transmits exciting light and detecting light in the single mode, and a ferrule 103 for expanding the outer diameter of the optical fiber 101 to the same value as that of the gradient index rod lens 102. The gradient index rod lens 102 and the optical fiber 101 are rigidly joined together by means of a sleeve 104.
    Type: Grant
    Filed: December 10, 2003
    Date of Patent: August 16, 2005
    Assignees: Nippon Sheet Glass Co., Ltd., Kanagawa Academy of Science & Technology
    Inventors: Jun Yamaguchi, Akihiko Hattori, Takehiko Kitamori, Manabu Tokeshi
  • Patent number: 6891619
    Abstract: An improved turbidity sensor is provided with a pair of spaced apart arms respectively housing a light transmitter and a light receiver, with the receiver adapted to sense the light from the transmitter and generate a corresponding output signal. The opposing surfaces of the arms adjacent the transmitter and receiver are flame treated for approximately one second each so as to raise the surface energy such that bubble-induced noise is reduced or eliminated for a more accurate output signal.
    Type: Grant
    Filed: April 19, 2002
    Date of Patent: May 10, 2005
    Assignee: Maytag Corporation
    Inventors: Anthony L. Durfee, John H. Miilu
  • Patent number: 6881760
    Abstract: A method for monitoring the solids content in a Fischer-Tropsch product to allow subsequent changes in the Fischer-Tropsch process to prevent downstream problems. The method comprises irradiating the Fischer-Tropsch product with light and measuring the light transmitted through the Fischer-Tropsch product to determine the solids content in the Fisher-Tropsch product.
    Type: Grant
    Filed: July 16, 2004
    Date of Patent: April 19, 2005
    Assignee: Chevron U.S.A. Inc.
    Inventors: Ben Smith, Richard Aviani, Dennis J. O'Rear
  • Patent number: 6771373
    Abstract: Sensor for household appliances, in particular washing machines and dishwashers, comprising a housing 2 which comprises first and second housing fingers 8, 10 extending from a basis, a first optical element 12 being arranged in the first housing finger 8, a second optical element 14 which is arranged in the second housing finger 10 wherein a sensing beam 16 transmitted and received by the optical elements 12, 14 is propagating between the housing fingers 8, 10 external to the housing 2 for sensing of the turbidity of a cleaning medium at least partially surrounding the housing 2, and a temperature sensor 18 which is arranged in the second housing finger 10 in a greater distance from the basis 4 compared to the second optical element 14 for sensing the temperature of the cleaning medium.
    Type: Grant
    Filed: March 28, 2002
    Date of Patent: August 3, 2004
    Assignee: Elektromanufaktur Zangenstein Hanauer GmbH & Co. KGaA
    Inventors: Johann Schenkl, Georg Wilhelm
  • Patent number: 6768549
    Abstract: An analyzer apparatus includes a receptacle for an ampoule, an analysis system, an incubator, and a master control. The analysis system includes a light source and a photodetector positioned such that the light from the light source passes through the receptacle. The master control includes a display, a timer, and a memory provided with a look-up table. During operation, an ampoule containing a sample and an indicator which changes color when a certain level of biological activity is present in the sample is placed within the receptacles. The analysis system is operated to transmit light at the predetermined wavelength through the ampoule to the detector, and a maximum amount of light passing through the ampoule is logged. The incubator is operated to heat the receptacle and the ampoule therein to a desired test temperature and the timer is started. The analysis system periodically transmits light through the ampoule.
    Type: Grant
    Filed: June 8, 2000
    Date of Patent: July 27, 2004
    Inventors: John Edward Pfeifer, Peter E. Rising
  • Patent number: 6727990
    Abstract: At the separation of different minerals from the ores by means of flotation, it belongs to known art to monitor a larger part of the uppermost froth layer in the flotation cell by means of a video camera whose signals are analyzed in a digital computer, in order to detect the structure and color of the froth. The new method and apparatus direct and limit the monitoring and analysis to the surface of the floated material within a fixed, narrow strip which is parallel to the overflow edge of the cell and within which the material surface passing it is homogeneous in the stationary state. The said strip thus presents a renewing sample of the surface at the location in question, which sample represents the stationary state in the average sense. The strip is subjected to homogeneous illumination, which may meet the strip at different angles depending on the primary object, i.e. whether the color or structure is being determined.
    Type: Grant
    Filed: April 10, 2002
    Date of Patent: April 27, 2004
    Inventor: Antti Niemi
  • Patent number: 6704110
    Abstract: Pulsed light of predetermined wavelengths or modulated light of predetermined frequencies from a light source 5 is injected into a scattering medium 1 as a measured object and output light therefrom is detected by a photodetector 7. Further, measuring methods and apparatus are configured so as to process and compute internal information by a signal processing unit 8 and an computation processing unit 9, and the internal information of the scattering medium 1 is obtained by calculating the difference between absorption coefficients by use of the time integrated spectroscopy (TIS method) and the phase modulation spectroscopy (PMS method) based on the MBL law in accordance with a spectroscopy method (MVS method) making use of a mean pathlength and a variance, or physical quantities equivalent thereto. This enables the internal information of the scattering medium to be measured accurately and quickly.
    Type: Grant
    Filed: May 4, 2001
    Date of Patent: March 9, 2004
    Assignee: Hamamatsu Photonics K.K.
    Inventor: Yutaka Tsuchiya
  • Publication number: 20030197868
    Abstract: An improved turbidity sensor is provided with a pair of spaced apart arms respectively housing a light transmitter and a light receiver, with the receiver adapted to sense the light from the transmitter and generate a corresponding output signal. The opposing surfaces of the arms adjacent the transmitter and receiver are flame treated for approximately one second each so as to raise the surface energy such that bubble-induced noise is reduced or eliminated for a more accurate output signal.
    Type: Application
    Filed: April 19, 2002
    Publication date: October 23, 2003
    Inventors: Anthony L. Durfee, John H. Miilu
  • Publication number: 20030184754
    Abstract: The invention relates to a method for optical measuring of the solid substance content in a fluid. Said method uses periodical flushing of an optical sensor (1) with a combination of pressurized air and water in the form of a jet. Said jet exerts a very powerful cleaning effect without causing scratches on measuring surfaces of glass or similar materials. The invention also relates to a system for performing such flushing in connection with measuring.
    Type: Application
    Filed: March 21, 2003
    Publication date: October 2, 2003
    Inventors: Sven Bernhardsson, Johan Llljha, Leif Pellas
  • Publication number: 20030142316
    Abstract: Sensor for household appliances, in particular washing machines and dishwashers, comprising a housing 2 which comprises first and second housing fingers 8, 10 extending from a basis, a first optical element 12 being arranged in the first housing finger 8, a second optical element 14 which is arranged in the second housing finger 10 wherein a sensing beam 16 transmitted and received by the optical elements 12, 14 is propagating between the housing fingers 8, 10 external to the housing 2 for sensing of the turbidity of a cleaning medium at least partially surrounding the housing 2, and a temperature sensor 18 which is arranged in the second housing finger 10 in a greater distance from the basis 4 compared to the second optical element 14 for sensing the temperature of the cleaning medium.
    Type: Application
    Filed: March 28, 2002
    Publication date: July 31, 2003
    Inventors: Johann Schenkl, Georg Wilhelm
  • Patent number: 6594017
    Abstract: The invention relates to a device and a method for moving a measuring sensor (2) into and out of a pressurized or flow-through conduit (1) or a pressurized or flow-through vessel. The device according to the invention comprises, in this case, a holding element (3) for holding the measuring sensor (2), a guiding element (4) for guiding the holding element (3) and a volume element (5). The holding element (3) can be moved in such a way that the measuring sensor (2) held by the holding element comes to rest in a position completely in the volume element (5). In this position, the measuring sensor (2) can be removed from the holding element (3). With the aid of the device according to the invention and the method according to the invention, it is possible, in particular, to measure the cleanliness of a conduit system blown out with a fluid, without the blow-out operation being interrupted.
    Type: Grant
    Filed: September 28, 2000
    Date of Patent: July 15, 2003
    Assignee: Alstom
    Inventor: Joachim Menden
  • Patent number: 6565815
    Abstract: The present invention provides a reaction vessel and apparatus for performing heat-exchanging reactions. The vessel has a chamber for holding a sample, the chamber being defined by a plurality of walls, at least two of the walls being light transmissive to provide optical windows to the chamber. The apparatus comprises at least one heating surface for contacting at least one of the plurality of walls, a heat source for heating the surface, and optics positioned to optically interrogate the chamber while the heating surface is in contact with at least one of the plurality of walls. The optics include at least one light source for transmitting light to the chamber through a first one of the light transmissive walls and at least one detector for detecting light exiting the chamber through a second one of the light transmissive walls.
    Type: Grant
    Filed: July 25, 2000
    Date of Patent: May 20, 2003
    Assignee: Cepheid
    Inventors: Ronald Chang, Lee A. Christel, Gregory T. A. Kovacs, William A. McMillan, M. Allen Northrup, Kurt E. Petersen, Farzad Pourahmadi, Steven J. Young, Robert Yuan, Douglas B. Dority
  • Patent number: 6551521
    Abstract: A highly accurate sensor for monitoring the color density of various etchants in etchant regeneration systems is disclosed. The sensor comprises a Pyrex or equivalent tubular sensing chamber and a light cell housing surrounding a portion of the chamber. The chamber contains a rodlike extension extending into the interior of the chamber. The housing accommodates a light source, preferably an LED or laser, and a photodetector, optically coupled through an aperture in the housing. Two such sensors are used in a regeneration apparatus attached to an etching machine used for etching copper, iron, stainless steels or other materials. In another embodiment, multiple sensors are used to detect multiple constituents of the etchant depending on the material being etched and the etchant used. A system employing two sets of sensors is particularly applicable to etchants that pass one color of light well when fully regenerated and another color of light well when fully spent.
    Type: Grant
    Filed: July 27, 2000
    Date of Patent: April 22, 2003
    Assignee: Oxford Vue, Inc.
    Inventors: Philip Culpovich, David Flynn
  • Patent number: 6519025
    Abstract: A method and apparatus that allows accurate spectrophotometric determination of the concentrations of various hemoglobin species in whole undiluted blood. The invention employs 1) an optical apparatus designed to maximize the true optical absorbance of whole blood and to minimize the effects of light scattering on the spectrophotometric measurements of the concentrations of various constituent components, and 2) methods to correct the hemoglobin concentration measurements for light scattering and for the effects of the finite bandwidth of the substantially monochromatic light. In the optical apparatus optical parameters, such as sample thickness, detector size, sample-to-detector distance, wavelengths, monochromicity, and maximum angle of light capture by detector, are selected so as to minimize the contribution of light scattering and to maximize the contribution of true optical absorbance.
    Type: Grant
    Filed: July 10, 2001
    Date of Patent: February 11, 2003
    Assignee: Board of Regents, The University of Texas System
    Inventors: Albert Pitt Shepherd, John M. Steinke
  • Publication number: 20030017079
    Abstract: A high-efficiency, high-sensitivity absorbance detection system in a lab-on-a-chip is provided. The absorbance detection system includes detection cells having an optical pathlength ten times and/or much longer than the width of a separation channel to improve detection sensitivity, lens structures for collimating light in the detection cells, and slit structures for preventing scattered light from entering detectors. The detection cells, the lens structures, and the slit structures of the absorbance detection system are fabricated and integrated in a lab-on-a-chip. The absorbance detection system exhibits excellent absorption efficiency, detection limit, and linearity, compared to existing absorbance detection systems, and can be applied for the detection of a variety of samples. The absorbance detection system does not need labeling of the samples which saves time and costs. The absorbance detection system can be used effectively in detecting trace compounds with a high sensitivity.
    Type: Application
    Filed: July 18, 2002
    Publication date: January 23, 2003
    Applicant: Pohang University of Science and Technology Foundation
    Inventors: Jong Hoon Hahn, Kyung Won Ro, Bong Chu Shim, Kwanseop Lim
  • Patent number: 6501072
    Abstract: The optical density of an oil sample at a plurality of wavelengths over a plurality of different (typically decreasing) pressures is monitored and used to find the size of agglomerated asphaltene particles which are precipitating from the oil sample. The optical density information used in finding the particle size is preferably optical density information relating to the scattering of light due to the asphaltene particles only. Thus, baseline optical density information of the oil sample at a high pressure is subtracted from optical density information obtained at test pressures at each wavelength of interest. Asphaltene particles of a radius of one micron and smaller were found to be powdery, while asphaltene particles of a radius of three microns and larger were found to include paving resins. The precipitation of asphaltenes is reversible by increasing the pressure under certain circumstances.
    Type: Grant
    Filed: January 29, 2001
    Date of Patent: December 31, 2002
    Assignee: Schlumberger Technology Corporation
    Inventors: Oliver C. Mullins, Abul Jamaluddin, Nikhil B. Joshi
  • Patent number: 6493567
    Abstract: A method and device for the continuous real-time monitoring of relative blood volume change, based on registration of blood hemoglobin concentration, during long periods of time, such as dialysis session. A method and device for cardiac output measurement, based on optical dilution technique, during dialysis, surgeries, intensive care procedure. The effects of blood electrolyte composition change which result in a change of light beam geometry are eliminated by the relative orientation between a light source and a single light or photodetector. An illumination axis and a detection axis are oriented in an offset, non collinear configuration at a sufficient angle to substantially eliminate the scattering properties of the blood. A primary implementation of the device is extracorporeal paths, such as hemodialysis tubing systems, artery-vein extracorporeal artificial shunts, other extracorporeal systems. It also may be applied to vessels, tissues or to body parts being capable of transillumination.
    Type: Grant
    Filed: March 20, 2000
    Date of Patent: December 10, 2002
    Assignee: Transonic Systems, Inc.
    Inventors: Nikolai M. Krivitski, Dimitry Starostin
  • Patent number: 6490039
    Abstract: Techniques and devices for sensing a sample by using a whispering gallery mode resonator.
    Type: Grant
    Filed: August 8, 2001
    Date of Patent: December 3, 2002
    Assignee: California Institute of Technology
    Inventors: Luftollah Maleki, Vladimir Ilchenko
  • Patent number: 6482652
    Abstract: An automated particle sorter having a fluid flow path, which places single biological particles in an optical cuvette. An exciting light irradiation system having a light source emits a source of light through the cuvette. The light excites a fluorescent substance present on the particle, and the emitted light is detected by a light detection apparatus containing at least two detection elements for measuring the fluorescence emitted from the fluorescent substance. A light separation element separates the fluorescence from the exciting light. A data processor compares the signal received from the fluorescent light; and from the background autofluorescent signal, and according to pre-set parameters, controls the position of a collection conduit between two set points. The first being a collection set point for the collection of objects having a first phenotype and the second being a set point for the collection of objects having a second phenotype.
    Type: Grant
    Filed: March 21, 2001
    Date of Patent: November 19, 2002
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Eileen Furlong, David Profitt, Matthew Scott
  • Patent number: 6465802
    Abstract: A flow cell for obtaining information on particles suspended in sample fluid, wherein an irradiation region M is defined in the flow cell for functioning as a particle detection portion to be irradiated with a laser beam La, and a wall portion of the flow cell is so adapted and arranged that the laser beam La becomes incident upon an outer wall surface of the flow cell 1 at a predetermined angle &thgr;1 (&thgr;1≠0°) and exits from an inner wall surface 5b of the flow cell 1 into the sample fluid 6 at a refraction angle 0°. Further the wall portion 5 of the flow cell 1 is so arranged that the laser beam La becomes incident upon a boundary surface between the sample fluid and the opposite inner wall surface of the flow cell 1 at a predetermined incident angle &agr; (&agr;≠0°) after being incident upon the sample fluid and passing through the irradiation region M.
    Type: Grant
    Filed: March 17, 2000
    Date of Patent: October 15, 2002
    Assignee: Rion Co., Ltd.
    Inventor: Tomonobu Matsuda
  • Patent number: 6426213
    Abstract: A sperm analysis system has a sperm sample carrier (20) and “reader” module. The sperm sample carrier includes: 1) a shank (24) defining a chamber (26) with an opening for ingress and egress of a sperm sample; a manually operated pump (28) for aspirating a sample of sperm into the chamber (26), and a plurality of distinct photon paths (34) intersecting and passing through the chamber (26). The module includes: a processor responsive to an actuation signal from an operator, a photon source, e.g.
    Type: Grant
    Filed: January 9, 2001
    Date of Patent: July 30, 2002
    Assignee: Progeny Systems, LLC
    Inventor: Henry L. Eisenson
  • Patent number: 6404494
    Abstract: An integrating nephelometer is modified to measure 180° backscattering from an aerosol. A laser is mounted outside a housing of the nephelometer and produces a coherent light beam that is directed into a sample volume of the nephelometer, substantially along an optical sensing axis (<4° off the optical axis). Light from the laser beam that is reflected by an aerosol travels back along the optical sensing axis toward a photomultiplier tube (PMT) light detector, which thus produces a signal indicative of the backscattering from the aerosol. A portion of the laser beam is conveyed into the housing of the nephelometer through an optical fiber for use as a reference beam. A motor-driven chopper disk disposed across the optical axis is divided into an open sector, a calibration sector (with a very low transmission), and a flat black light absorbing sector.
    Type: Grant
    Filed: December 22, 1999
    Date of Patent: June 11, 2002
    Assignee: University of Washington
    Inventors: Sarah J. Masonis, Theodore L. Anderson, Robert J. Charlson
  • Patent number: 6370342
    Abstract: A toner concentration sensor has a pair of optical members for optically coupling a light emitting device and a photodetector. The optical members are disposed with a gap therebetween for introducing liquid developer to measure transparency of the liquid developer and to evaluate the toner concentration. The optical member is coated with a chemical adsorption film as an anti-fouling film including at least one of organosilane compounds expressed by the following formulas: wherein each of R1, R2 and R3 represents an organic functional group, and each of X1, X2 and X3 is a desorption group.
    Type: Grant
    Filed: October 16, 2000
    Date of Patent: April 9, 2002
    Assignee: NEC Corporation
    Inventor: Tomohiro Masumura
  • Patent number: 6361956
    Abstract: A method for measuring the end point and for monitoring the real time kinetics of a bioaffinity reaction in biological fluids and suspensions, employing microparticles as bioaffinity binding solid phase, biospecific reagent labelled with a fluorescent label and a fluorescence detection system which is based on two-photon fluorescence excitation, contacting the analyte, the labelled reagent and the solid phase simultaneously, focusing a two-photon exciting laser beam into the reaction suspension and measuring the fluorescence signal emitted by the microparticles from one particle at a time when they randomly float through the focal volume of the laser beam. In this method the signal is monitored kinetically to obtain information about the analyte concentration before the reaction approaches the highest point of the response. Since the growth rate of the signal intensity is directly proportional to the analyte concentration, the analyte concentration can be predicted in the initial phase of the reaction.
    Type: Grant
    Filed: May 5, 1999
    Date of Patent: March 26, 2002
    Inventors: Pekka Hänninen, Erkki Soini, Juhani Soini
  • Patent number: 6342397
    Abstract: A homogeneous biospecific assay method for an analyte in solution or in a biological suspension, in which a biospecific reagent competitively binding an analyte and a ligand labeled with a fluorescent molecule, is reacted with and bound to a solid phase, and in which the free labeled ligand is extracted is excited with two-photon excitation by focusing a laser beam suitable for two-photon excitation into the sample volume; and the concentration of the analyte is calculated based on the photon emission contributed by the free labeled ligand.
    Type: Grant
    Filed: October 31, 2000
    Date of Patent: January 29, 2002
    Inventors: Erkki Soini, Pekka Hänninen
  • Patent number: 6315955
    Abstract: The present invention includes a method and an apparatus for the quantitative determination of particles in fluid. The apparatus of the invention includes an emitters set of one or more light emitters, in combination with a detector set of one or more light detectors sensitive to the output of the emitters. During analysis of the sample, data from a plurality of signal paths between the emitter and detector sets are gathered. This information is subsequently evaluated by comparison with known data for different fluid particle contents. Some differentiation between different particles in a fluid sample are possible in many embodiments. Typical uses include analysis of milk and dairy fluids, blood samples, lubricants, suspensions of pigments, etc.
    Type: Grant
    Filed: January 16, 1998
    Date of Patent: November 13, 2001
    Assignee: DeLaval International A.B.
    Inventor: Cornelis Klein
  • Patent number: 6263725
    Abstract: A method and apparatus are for characterizing colloids by successive measurements of light attenuation or emission of a colloidal mixture at two or more temperatures and at one or more wavelengths. The difference or ratio of the measurements at two or more temperatures provides a measure of the colloidal stability with respect to temperature. The amount of certain organic colloids that are unstable to temperature variation may be determined from the magnitude of the measurement change. In appropriate mixtures, this method provides a means to distinguish components of colloidal substances based. This method may be specifically applied to determining the stability and concentration of colloidal pitch or wood resin in a pulp or paper process water. In this context it may be used to distinguishing colloidal pitch or wood resin from talc, clay, carbon black, or other fillers.
    Type: Grant
    Filed: September 18, 1998
    Date of Patent: July 24, 2001
    Assignee: Alberta Research Council Inc.
    Inventors: Theodore M. Garver, Kenneth Bough
  • Patent number: 6262798
    Abstract: A method and apparatus that allows accurate spectrophotometric determination of the concentrations of various hemoglobin species in whole blood without hemolysis or dilution. To overcome the complex optical properties of whole blood, the invention employs 1) an optical apparatus designed to maximize the true optical absorbance of whole blood and to minimize the effects of light scattering on the spectrophotometric measurements of the concentrations of various constituent components, and 2) methods to correct the hemoglobin concentration measurements for light scattering and for the effects of the finite bandwidth of the substantially monochromatic light.
    Type: Grant
    Filed: September 29, 1992
    Date of Patent: July 17, 2001
    Assignee: Board of Regents, The University of Texas System
    Inventors: A. P. Shepherd, John M. Steinke
  • Patent number: 6229908
    Abstract: A method and an ignition interlock for preventing operation of equipment when an operator's blood-alcohol content is above a threshold value. The interlock has a blood-alcohol detector that measures intensities of wavelengths of light emerging from a finger. A microprocessor correlates these intensities with the finger's blood-alcohol content, determines whether this content is above a threshold level, and prevents the equipment from operating unless the blood-alcohol content is below the threshold. The interlock also has a fingerprint image generator which reflects light of the finger and scans the fingerprint to form a scanned image. The microprocessor compares this scanned image to a prestored image of a principal operator and compares the two images to determine whether the images match. The fingerprint and blood-alcohol analyses occur substantially simultaneously.
    Type: Grant
    Filed: April 22, 1997
    Date of Patent: May 8, 2001
    Inventors: Dean Stockett Edmonds, III, Jon William Hopta
  • Patent number: 6172750
    Abstract: There is provided a method or system that utilizes fluorescence in a sample that would otherwise yield inaccurate results due to spectrally interfering components. The system quantifies spectral interference of a fluorescent label.
    Type: Grant
    Filed: September 18, 1997
    Date of Patent: January 9, 2001
    Inventor: Robert W. Brocia
  • Patent number: 6119510
    Abstract: The invention provides an improved process for determining the characteristics of dispersed particles. The process involves directing waves (acoustic or light) into a dispersion, and measuring the attenuation of the waves for particular frequencies to provide an attenuation spectrum. The measured attenuation spectrum is then compared to a set of theory-based calculated attenuation spectra to determine the particle size distribution corresponding to the measured attenuation spectrum. Unlike previous processes, the particle size distribution is capable of being accurately determined by a single inversion algorithm, thus eliminating burdensome steps of previous methods.
    Type: Grant
    Filed: August 31, 1998
    Date of Patent: September 19, 2000
    Assignee: Lucent Technologies Inc.
    Inventors: Melanie Carasso, Sanjay Patel, Jorge Luis Valdes, Christopher Alan White
  • Patent number: 6089078
    Abstract: A process provides for measuring particles in suspension in a liquid in an particle response analyzing instrument comprising a cytometric flow cell head, wherein the suspension is injected into the head at an injection rate, the head comprises one or several sensors which deliver responses when measuring particles injected therein and which yield a significant response for a set measurement frequency range. The response is processed and used to generate results in the instrument. When a first response is significant, measurements continue at the same injection rate in order to obtain a result thats likely to continue being a significant response during subsequent measurements and, conversely, when the first response is not significant, the injection rate is modified in order to bring the response within a significant response range and to continue with measurements in order to obtain a result.
    Type: Grant
    Filed: August 6, 1998
    Date of Patent: July 18, 2000
    Assignee: Hycel Diagnostics
    Inventors: Jean-Claude Chelveder, Fabien Kolly, Laurent Morellet
  • Patent number: 6046805
    Abstract: An apparatus and a method for transfusing liquid specimen for an optical characteristic measurement, which is excellent in operability and handling, and capable of introducing a liquid specimen into a sample cell without detaching the cell from an optical characteristic measurement apparatus and without bubbling, as well as a polarimeter are disclosed. According to the present invention, the specimen can be introduced into the sample cell without detaching the sample cell from the equipment, in a way of connecting the bottom of the sample cell with a reservoir for temporarily accommodating the liquid specimen through a tubular path, then varying a difference in the levels of the sample cell and the reservoir, or a way of using a syringe arranged in the tubular path. At expelling the specimen from the sample cell, the specimen is transfused into the reservoir in a similar manner.
    Type: Grant
    Filed: September 23, 1998
    Date of Patent: April 4, 2000
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Tatsurou Kawamura, Jinsei Miyazaki
  • Patent number: 6016203
    Abstract: The invention comprises a gas sensor (1), designed to enable a measurement of a gas sample that is enclosed in a cavity (2), having the shape of a block (50); the wall or wall sections of the cavity exhibiting highly-reflective properties for light. Said cavity contains means (51) for incoming rays of light, and means (52) for outgoing rays of light. Said cavity (2) exhibits opposing surface sections (55a, 56a, 57a) that are designed and coordinated so that the incoming rays of light (60) are arranged to pass--without reflecting, or without appreciably reflecting within a plane (the x-z plane)--across said cavity (2) to a concave mirror surface (55a) that is oriented in a right angle to the plane; that said rays of light are arranged to pass reflected across said cavity (2) in a plane of the mirror surface to the other surfaces (56a, 57a), thereby forming a measuring path before the reflected beams of light are aimed towards said means (52) for exiting light.
    Type: Grant
    Filed: April 22, 1998
    Date of Patent: January 18, 2000
    Inventor: Hans Goran Evald Martin
  • Patent number: 6003678
    Abstract: A disposable first tube (68) extends axially through, and is detachably connected to, an annular main body (10'). An input piezo electric element (38) is attached to a first end of the tubular main body (10'). A second, sensor piezo electric element (40) is attached to the opposite end of the main body (10'). A nozzle (20') having a nozzle passageway (110) and a discharge opening (112) is detachably secured to an outlet end of the first tube (68). A second tube (102) within the first tube (68) delivers a core liquid to the nozzle passageway (110). A sheath liquid is delivered through a space in the first tube (68) surrounding the second tube (102). The nozzle passageway (110) forms the core and sheath liquids into a small diameter jet stream. Electrical energy is delivered to the input piezo electric element (38), to vibrate the nozzle (20') and break the jet stream into droplets.
    Type: Grant
    Filed: October 13, 1998
    Date of Patent: December 21, 1999
    Assignee: University of Washington
    Inventor: Gerrit J. Van den Engh