Abstract: A method for forming a structured polymeric film having a plurality of longitudinally spaced structured on both sides of the structured polymeric film is described. The method includes: providing a rotatable tool having an outer circumferential surface, the outer circumferential surface including a plurality of tool projections; providing a nip roll having a smooth conformable outer circumferential surface opposed to the outer circumferential surface of the tool; introducing a polymer layer into a nip between the tool and the nip roll; pressing the polymer layer between the tool and the nip roll to form web recesses into a first side of the polymer layer and web projections extending away from an opposing second side of the polymer layer, with the tool projections on the circumferential surface of the tool and form a structured web; and removing the structured web from the tool. Sample processing articles are also described.

Abstract: A micro fluidic device comprises a laminate structure, comprising a plurality of individual layers. At least one layer comprises a micro fluidic channel structure and at least on one side of said layer a further layer is arranged comprising a three-dimensional (3D) micro structure such that the 3D micro structure is influencing a flow characteristic of a fluid within the micro fluidic channel structure.

Abstract: A microfluidic manipulation system is provided that includes a blade for manipulating deformable material and at least one movable support that is capable of moving the blade into contact with a microfluidic device including a deformable feature. When the microfluidic device is operatively held by a holder, a movable support can position the distal end of the blade relative to the microfluidic device and move the contact tip surface of the blade such that it deforms the deformable feature.

Abstract: The methods for producing microchannel chips of the present invention comprise the steps of shielding substrate surfaces on which groove-like channels have been formed, using a mask that exposes the channels, and then forming polymer membranes on the exposed surfaces of the substrates; and the step of laminating cover materials onto the side of the substrate surfaces on which the channels have been formed.

Abstract: A device and method are provided for facilitating extraction of a fraction from a biological sample. The biological sample includes non-desired material and a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a phase-gate zone for receiving an isolation buffer therein. An output zone receives a reagent therein. A force is movable between a first position adjacent the input zone and a second position adjacent the output zone. The force urges the fraction-bound solid phase substrate from the input zone, through the phase-gate zone and into the output zone.

Abstract: Protein crystallization screening and optimization droplet actuators, systems and methods are provided. According to one embodiment, a screening droplet actuator is provided and includes: (a) a port for introduction of one or more crystallization reagents and/or one or more protein solutions; and (b) a substrate including: (i) an array of two or more mixing wells; and (ii) electric field mediated microfluidics for moving droplets comprising the crystallization reagents and protein solutions into the mixing wells. Optimization droplet actuators, systems including screening droplet actuators, methods of screening protein crystallization conditions, and methods of testing conditions for growing a crystal are also provided.

Abstract: A microchannel has a substrate body; a separation channel which is formed within the substrate; at least two (first and second) connection ports which are formed at one end of the separation channel close to the thickness direction perpendicular to the longitudinal direction of the substrate body; and first and second branch channels connected respectively to the first and second connection ports. When the substrate body is perpendicular to gravity, a blood sample containing blood cells and plasma, flows from one end of the separation channel. Before the blood sample gets to the other end of the separation channel, the blood cells in the blood sample settle out. The blood cells having settled out are then separated into the branch channel.

Abstract: The invention is directed to microfluidic devices comprising at least two processing channels, wherein each of the processing channels comprises an inlet, an outlet, and a high-flow-resistant and hydrophilic conduit; a distributing channel, wherein the distributing channel comprises an upstream end and a downstream end, and is in fluid communication with each inlet of the processing channels via the high-flow-resistant and hydrophilic conduit; and a flushing channel, wherein the flushing channel comprises an upstream end and a downstream end, and is in fluid communication with each outlet of the processing channels. The invention also provides methods of using the microfluidic devices.

Abstract: A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an output layer having a plurality of capillaries, wherein a first grouping of the capillaries is separated from a second grouping by a hydrophobic feature. Each of the plurality of capillaries can comprise an inlet and an outlet. A funnel assembly can comprise a funnel member sized to receive the assay. The funnel member can comprise an outlet for delivering a fluid bead of the assay along a top surface of the output layer and in fluid communication with each of the plurality of capillaries such that a portion of the fluid bead can be drawn within at least some of the plurality of capillaries in response to capillary force. The funnel assembly and the output layer can be moveable relative to each other between a first position and a second position to draw the fluid bead across the top surface.

Abstract: Multiwell droplet actuators, systems and methods are provided. According to one embodiment, a substrate is provided and comprises: (a) one or more input ports for introduction of one or more reagents and/or samples; (b) a regular array of processing wells; and (c) a network of droplet transport pathways comprising pathways that provide direct or indirect droplet transport from each of the input ports to each of the one or more processing wells. Varying droplet actuators and systems related thereto are also provided.

Abstract: A microfluidic assembly (1;57) has at least one microfluidic flow path (17,27,29,35,38;82,85,95) and at least one inlet port (11,13,15;81,97,101) coupled to the flow path (17,27,29,35,38;82,85,95). The microfluidic assembly (1;57) has a first microfluidic device (7;63) that executes a microfluidic process and has a second microfluidic device (9;61). The microfluidic assembly (1;57) has an interface (5;65). The interface (5;65) couples the first microfluidic device (7;63) and the second microfluidic device (9;61).

Abstract: The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.

Abstract: A measuring unit includes a quantifying section for quantifying a sample in volume, a main channel communicating with the quantifying section, an analyzing section provided in the main channel for analyzing the quantified sample, and a pressure introduction port communicating with the main channel for introducing a pressure into the main channel to transport the sample from the quantifying section to the analyzing section, wherein the analyzing section comprises at least one of an electrical characteristic measuring section for measuring an electrical characteristic of the sample and an optical characteristic measuring section for measuring an optical characteristic of the sample.

Abstract: An absorption spectrometric analysis microchip with a chamber for holding a sample, a chamber for holding a reagent which reacts with this sample, a mixing chamber for mixing the reagent with the sample with the formation of a mixture and a sensing part with a sensing chamber for holding the mixture with a light incidence surface for the entry of light into the sensing chamber and a light exit surface for emergence of light from the sensing chamber. At least one of the light incidence surface and light exit surface is located in a recess area of the sensing part.

Abstract: The systems and methods disclosed herein include a microfluidic system, comprising a pneumatic manifold having a plurality of apertures, and a chip manifold having channels disposed therein for routing pneumatic signals from respective ones of the apertures to a plurality of valves in a microfluidic chip, wherein the channels route the pneumatic signals in accordance with a configuration of the plurality of valves in the microfluidic chip.

Abstract: A measuring chip is configured for separating and measuring a target component in a sample by rotation around first and second axes of rotation. The measuring chip includes a centrifugal separation tube that centrifugally separates the target component from the sample by rotating the measuring chip around the first axis of rotation; a first holding section installed in the bottom of the centrifugal separation tube, wherein non-target components other than the target component in the sample are introduced therein by rotation around the first axis of rotation, and the first holding section holds the non-target components during rotation around the second axis of rotation; and a measuring section connected to one end of the centrifugal separation tube that measures the non-target components introduced from the centrifugal separation tube by rotation around the second axis of rotation.

Abstract: A dual inlet microchannel device and a method for using the device to perform a flow-through kinetic assay are described. A microplate having an array of the dual inlet microchannel devices and in particular their specially configured flow chambers is also described. Several embodiments of the dual inlet microchannel devices and specially configured flow chambers are also described.

Abstract: Microstructured device for removable storage of small amounts of liquid with the following features: the device has a carrier; the device has a first cavity with at least one first section for storage of a small amount of liquid; the first section of the first cavity is molded into the carrier; the first section is closed with a cover element and a blocking element; the device has a means for transmission of a force from the cover element to the blocking element which destroys the connection between the blocking element and the carrier or destroys the blocking element itself so that the amount of liquid can be removed from the first section of the cavity.

Abstract: The invention relates to a pipetting apparatus (1) having a pipette tip (2) for aspirating and dispensing liquid samples; a pump (4) for generating negative pressure or positive pressure in the pipette tip (2) that is connected to the pipette tip (2) by means of a pump conduit (3); a fluid chamber (5) defined by the pipette tip (2) and/or the pump conduit (3); a measurement probe (6) functionally connected to the fluid chamber (5) for measuring the physical parameters resulting in this fluid chamber (5) during pipetting; and a device control system (7) with a processor (8), in which an activated computer program product enables the control system (7) of the pipetting apparatus (1) to individually accept or reject pipetted liquid samples on the basis of the physical parameters measured.

Abstract: The present invention relates to an analytical tool (1) comprising a substrate (10), a capillary (13) which is formed on the substrate (10) and into which a sample liquid is to be loaded by movement of the sample liquid in the capillary. The substrate (10) is provided with a liquid movement preventer for preventing the sample liquid loaded into the capillary (13) from moving further. Preferably, the liquid movement preventer includes a stepped portion (18B) projecting from the substrate or a recess provided at the substrate.

Abstract: An apparatus main body is loaded with a cartridge that contains a sensor and is capable of processing liquid contained in it without moving it from the inside to the outside of the cartridge. The cartridge is loaded into the apparatus main body in such a way that the direction of feeding liquid in the span of the flow channel where the sensor is arranged is vertical and the cartridge can be accessed from the apparatus main body from above.

Abstract: Disclosed herein is a method of: treating an organic polymer with an electron beam-generated plasma; exposing the treated polymer to air or an oxygen- and hydrogen-containing gas, generating hydroxyl groups on the surface of the polymer; reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group; and covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof.

Abstract: A microfluidic device [10] includes at least one reactant passage [26] and one or more thermal control passages defined therein, the one or more thermal control passages being positioned and arranged within two volumes [12,14] each bordered by a wall [18,20], the walls being generally planar and parallel to one another, the reactant passage positioned between said generally planar walls and defined by said generally planar walls and walls [28] extending between said generally planar walls, wherein the reactant passage comprises multiple successive chambers [34], each such chamber including a split of the reactant passage into at least two sub-passages [36], and a joining [38] of the split passages, and a change of passage direction, of at least one of the sub-passages, of at least 90 degrees.

Abstract: An analytical device according to the present invention comprises a liquid accommodating chamber 9 for accommodating a sample solution of a quantity required for analyzing; a volume measuring chamber 10 connected to the liquid accommodating chamber 9 with a connecting path 13 and disposed in the exterior of the liquid accommodating chamber 9 in a radial direction; an overflowing chamber 11 connected to the volume measuring chamber 10 for accommodating an excessive quantity of the sample solution; and a measuring cell 12 for transferring and measuring the sample solution measured in the volume measuring chamber 10; wherein an overflowing port 14 of the volume measuring chamber 10 is connected to a flow-in port 16 of the overflowing chamber 11 by a capillary path 17.

Abstract: Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.

Abstract: A “plug-n-play” modular microfluidic system is described herein which can be made by connecting multiple microfluidic components together to form a larger integrated system. For example, the modular microfluidic system includes a motherboard with interconnecting channels and integrated electrodes (or holes for electrodes to pass) which provide electronic connections to external data acquisition and system control devices. The modular microfluidic system can also include channel inserts (which are placed in the channels of the motherboard), heater units, actuator units, fitting components and microchips/modules with different functionalities which are placed on the motherboard.

Abstract: The microfluidic system is constituted of modules that comprise one microfluidic unit and one corresponding electric control unit each and that are retained on a rear panel unit next to each other in a row. To prevent the formation of accumulation of ignitable or toxic gas mixtures a fluid conduit for a rinsing fluid extends through the rear panel unit. Branches lead from said fluid conduit to the modules, and said branches flowing into respective distributor compartments that extend vertically across the module height in the modules. Said distributor compartments are delimited in relation to the interior of the respective module by a distributor panel that is provide with openings. The interior of the respective module comprises, on its lower or rear surface, an exit opening for the rinsing fluid.

Abstract: The compositions and methods described herein are designed to introduce functionalized microparticles into droplets that can be manipulated in microfluidic devices by fields, including electric (dielectrophoretic) or magnetic fields, and extracted by splitting a droplet to separate the portion of the droplet that contains the majority of the microparticles from the part that is largely devoid of the microparticles. Within the device, channels are variously configured at Y- or T junctions that facilitate continuous, serial isolation and dilution of analytes in solution. The devices can be limited in the sense that they can be designed to output purified analytes that are then further analyzed in separate machines or they can include additional channels through which purified analytes can be further processed and analyzed.

Abstract: In a microfluidic system, a magnetic clamp for sealing a flexible microchannel chip includes a base formed from a magnetically-attractable material. The base supports a window for viewing the face of the microfluidic chip. A ring with magnets uniformly distributed around it is disposed over the base. A transparent disk attached to the top of the ring has an inlet and an outlet for introducing and removing a fluid medium into a cavity defined the disk, the window, the center opening of the ring and the base. An elastomer cushion is attached to the inner surface of the disk. The magnetic force between the base and the magnets on the ring compresses the cushion against the microfluidic chip so that the microchannels are sealed against the window with a uniform and reproducible pressure.

Abstract: A method of making a microfluidic diagnostic device for use in the assaying of biological fluids, whereby a layer of adhesive in a channel pattern is printed onto a surface of a base sheet and a cover sheet is adhered to the base sheet with the adhesive. The layer of adhesive defines at least one channel, wherein the channel passes through the thickness of the adhesive layer.

Abstract: A microchip has a resin substrate, which is provided with a first surface whereupon a channel groove is formed and a second surface on the opposite side to the first surface, and the microchip also has a resin film bonded on the first surface. A projection area is larger than the area of the first surface of the resin substrate when the resin substrate is viewed from a direction orthogonally intersecting with the first surface. Thus, warpage of the microchip can be suppressed at the time of thermally bonding the resin substrate and the resin film by a roller.

Abstract: The present invention provides a photoresponsive gas-generating material that is to be used in a micropump of a microfluid device having fine channels formed therein, and is capable of effectively generating gases for transporting a microfluid in response to light irradiation and transporting the microfluid at an improved transport efficiency. The present invention also provides a micropump incorporating the photoresponsive gas-generating material. A photoresponsive gas-generating material 13 is to be used in a micropump having fine channels formed in a substrate, and comprises a photo-sensitive acid-generating agent and an acid-sensitive gas-generating agent, and a micropump 10 has the photoresponsive gas-generating material 13 housed therein.

Abstract: Provided are a microchip formed by joining a resinous film onto a resinous substrate, which enables the prevention of a leak due to peeling of a peripheral portion and prevention of deformation and clogging of a microchannel, and a method for manufacturing the same. The microchip comprises a resinous substrate including a first surface in which a channel groove is formed and a second surface on the side opposite to the first surface, and a resinous film joined to the first surface of the resinous substrate. A joint plane between the resinous substrate and the resinous film is composed of a central area including an area in which the channel groove is formed and a peripheral area corresponding to the outer periphery of the central area, the joint strength between the resinous substrate and the resinous film in the central area is larger than 0.098 N/cm, and the joint strength in at least part of the peripheral area of the joint plane is larger than the joint strength in the central area.