Micro-fluidic Device Patents (Class 422/502)
  • Patent number: 10350594
    Abstract: A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.
    Type: Grant
    Filed: March 5, 2018
    Date of Patent: July 16, 2019
    Assignee: Berkeley Lights, Inc.
    Inventors: Eric D. Hobbs, Justin K. Valley
  • Patent number: 10350599
    Abstract: A microfluidic exosome profiling platform integrating exosome isolation and targeted proteomic analysis is disclosed. This platform is capable of quantitative exosomal biomarker profiling directly from plasma samples with markedly enhanced sensitivity and specificity. Identification of distinct subpopulation of patient-derived exosomes is demonstrated by probing surface proteins and multiparameter analyzes of intravesicular biomarkers in the selected subpopulation. The expression of IGF-1R and its phosphorylation level in non-small cell lung cancer (NSCLC) patient plasma is assessed as a non-invasive alternative to the conventional biopsy and immunohistochemistry. Detection of ovarian cancer also is assessed. The microfluidic chip, which may be fabricated of a glass substrate and a layer of poly(dimethylsiloxane), includes a serpentine microchannel to mix a fluid and a microchamber for the collection and detection of exosomes.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: July 16, 2019
    Assignees: University of Kansas, Kansas State University Research Foundation
    Inventors: Zheng Zhao, Mei He, Yong Zeng
  • Patent number: 10345205
    Abstract: Sample application systems can include an extraction mechanism to remove a sample from sample containers, a sample vessel disposed on a deployment mechanism, where the deployment mechanism is arranged to move the sample vessel to receive a sample, an extraction mechanism washing station to wash the extraction mechanism, a sample applicator to remove a portion of the sample in the sample vessel and apply it onto a sample carrier, where the deployment mechanism can move the sample vessel to a sample application position, a sample vessel washing station to wash the sample vessel, where the deployment mechanism can move the sample vessel to the sample vessel washing station, a sample applicator washing station to wash the sample applicator after the sample has been dispensed onto the sample carrier, and a fluid control system to control flow of a fluid provided to the extraction mechanism and the sample applicator.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: July 9, 2019
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Brian J. McKeen, Eric D. Yeaton, James L. Dowling, David Zahniser, Stephen Conroy
  • Patent number: 10330919
    Abstract: A microfluidic system includes an electro-wetting on dielectric (EWOD) device and a control system that controls actuation voltages applied to the element array of the EWOD device to perform manipulation operations as to fluid droplets. The control system applies a sequence of actuation voltages to a portion of the array elements associated with a droplet to maintain the droplet in a desired droplet state corresponding to a predetermined droplet property. The sequence of actuation voltages includes an actuation-on period in which the portion of the array elements associated with the droplet is actuated and an actuation-off period in which the portion of the array elements associated with the droplet is not actuated, and the actuation-off period is non-zero. The control system may apply a sequence of actuation voltages comprising a predetermined duty cycle, and/or the actuation voltages may be applied in accordance with a sensor based intervention.
    Type: Grant
    Filed: March 31, 2017
    Date of Patent: June 25, 2019
    Assignee: Sharp Life Science (EU) Limited
    Inventors: Laura Huang, Tim Boyle
  • Patent number: 10295493
    Abstract: A sampling plate (1) for use in measuring a property of a fluid sample comprising a sample zone comprising at least two discrete testing zones (5, 6) for receiving different respective volumes of the fluid sample. Each testing zone presents a zone volume defined by a respective zone area and a different respective zone height being a dimension transverse to the zone area (A).
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: May 21, 2019
    Assignees: BRYTECH DESIGN & ENGINEERING PROJECT MANAGEMENT LIMITED, TAPE SPECIALITIES LIMITED
    Inventors: Matthew Bryan, Stuart Seagrave
  • Patent number: 10226767
    Abstract: A technique relates to a fluidic cell configured to hold a nanofluidic chip. A first plate is configured to hold the nanofluidic chip. A second plate is configured to fit on top of the first plate, such that the nanofluidic chip is held in place. The second plate has at least one first port and at least one second port. The second plate has an entrance hole configured to communicate with an inlet hole of the nanofluidic chip. The second port is angled above the first port, such that the first port and second port intersect to form a junction. The second port is formed to have a line-of-sight to the entrance hole, such that the second port is configured to receive input for extracting air trapped at a vicinity of the entrance hole.
    Type: Grant
    Filed: February 2, 2018
    Date of Patent: March 12, 2019
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Michael A. Pereira, Joshua T. Smith, Benjamin H. Wunsch
  • Patent number: 10196755
    Abstract: A gas phase nanowire growth apparatus including a reaction chamber, a first input and a second input. The first input is located concentrically within the second input and the first and second input are configured such that a second input fluid delivered from the second input provides a sheath between a first fluid delivered from the first input and a wall of the reaction chamber. An aerosol of catalyst particles may be used to grow the nanowires.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: February 5, 2019
    Assignee: SOL VOLTAICS AB
    Inventors: Greg Alcott, Martin Magnusson, Olivier Postel, Knut Deppert, Lars Samuelson, Jonas Ohlsson
  • Patent number: 10159976
    Abstract: The present invention is notably directed to method of fabrication of a microfluidic chip (1), comprising: providing (S1-S7) a substrate (10), a face (F) of which is covered by an electrically insulating layer (30); obtaining (S8) a resist layer (40) covering one or more selected portions (P1) of the electrically insulating layer (30), at least a remaining portion (P2) of said electrically insulating layer (30) not being covered by the resist layer; partially etching (S9) with a wet etchant (E) a surface of the remaining portion (P2) of the electrically insulating layer (30) to create a recess (40r) and/or an undercut (40u) under the resist layer (40); depositing (S10) the electrically conductive layer (50) on the etched surface (35), such that the electrically conductive layer reaches the created recess (40r) and/or undercut (40u); and removing (S11) the resist layer (40) to expose a portion (P1) of the electrically insulating layer adjoining a contiguous portion (P2) of the electrically conductive layer (50
    Type: Grant
    Filed: June 18, 2014
    Date of Patent: December 25, 2018
    Assignee: International Business Machines Corporation
    Inventors: Emmanuel Delamarche, Tobias Guenzler, Yuksel Temiz, Tino Treiber
  • Patent number: 10058869
    Abstract: A micro-fluidic device includes a plurality of heating elements on a substrate for heating the substrate. They define a plurality of temperature regions having distinct temperatures on the substrate. A flow feature layer is formed above the substrate to define a channel extending across the substrate through each temperature region. As fluid is repeatedly pumped within the channel, it flows from one temperature region to a next temperature region to undergo thermal cycling. Fluid wells reside in the flow feature layer under a cover and connect to the channel whereby fluid can dwell for diagnostic applications.
    Type: Grant
    Filed: March 21, 2016
    Date of Patent: August 28, 2018
    Assignee: Microfluidic MEMS Solution, LLC
    Inventors: Steven Wayne Bergstedt, Stephen Jacob DeMoor, Yimin Guan
  • Patent number: 10052607
    Abstract: An instrument and method for accelerating the solid phase synthesis of peptides are disclosed. The method includes the steps of deprotecting a protected first amino acid linked to a solid phase resin by admixing the protected linked acid with a deprotecting solution in a microwave transparent vessel while irradiating the admixed acid and solution with microwaves, activating a second amino acid, coupling the second amino acid to the first acid while irradiating the composition in the same vessel with microwaves, and cleaving the linked peptide from the solid phase resin by admixing the linked peptide with a cleaving composition in the same vessel while irradiating the composition with microwaves.
    Type: Grant
    Filed: June 2, 2017
    Date of Patent: August 21, 2018
    Assignee: CEM Corporation
    Inventors: Jonathan McKinnon Collins, Joseph Joshua Lambert, Michael John Collins
  • Patent number: 10024852
    Abstract: A reusable coating for a nanopore structure is disclosed herein. A nanopore structure includes a substrate comprising a nanochannel and a monolayer of a chemical compound disposed onto at least a portion of a surface of the nanochannel. The chemical compound forms a reversible bond with at least one analyte binding compound introduced into the nanochannel. Methods for making and using the reusable coating are also disclosed.
    Type: Grant
    Filed: November 4, 2013
    Date of Patent: July 17, 2018
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Natalie Gunn, Jose M. Lobez Comeras, Priscilla Rogers, John Wagner
  • Patent number: 10024851
    Abstract: A reusable coating for a nanopore structure is disclosed herein. A nanopore structure includes a substrate comprising a nanochannel and a monolayer of a chemical compound disposed onto at least a portion of a surface of the nanochannel. The chemical compound forms a reversible bond with at least one analyte binding compound introduced into the nanochannel. Methods for making and using the reusable coating are also disclosed.
    Type: Grant
    Filed: October 15, 2013
    Date of Patent: July 17, 2018
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Natalie Gunn, Jose M. Lobez Comeras, Priscilla Rogers, John Wagner
  • Patent number: 9943843
    Abstract: The subject of the present invention is a microfluidic circuit in which are defined microchannels able to contain fluids and including at least one device for forming drops of a solution, guiding the drops to a storage zone in which one of the drops can be brought into contact with a drop of another solution, the walls of the microchannel portion forming the first drop-formation device diverging so as to cause drops of the first solution to detach under the effect of the surface tension of the first solution; the first guide include wall portions of the microchannels that diverge so as to cause the drops to move along under the effect of the surface tension of the first solution.
    Type: Grant
    Filed: October 8, 2013
    Date of Patent: April 17, 2018
    Assignees: ECOLE POLYTECHNIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Charles Baroud, Paul Abbyad, Etienne Fradet, RĂ©mi Dangla
  • Patent number: 9890424
    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.
    Type: Grant
    Filed: June 17, 2015
    Date of Patent: February 13, 2018
    Assignee: Life Technologies Corporation
    Inventors: Jonathan Schultz, Todd Roswech, Jon A. Hoshizaki, Albert L. Carrillo, James A. Ball
  • Patent number: 9886041
    Abstract: A method for configuring a fluid control unit including the provision of a processing device with information on the fluid control unit, the moving device and fluid control valves, the determination of possible basic states of the moving device for the fluid control valves and/or of possible movement sequences, and the selection of a basic state and/or of a movement sequence by a user, the transmission, of a respective configuration file to the fluid control unit including open- and/or closed-loop control parameters for the selection of the fluid control valves, the use of the open- and/or closed-loop control parameters from the configuration file in the fluid control unit for selecting the fluid control valves for setting the selected basic state and/or for carrying out the selected movement sequence.
    Type: Grant
    Filed: January 18, 2012
    Date of Patent: February 6, 2018
    Assignee: FESTO AG & CO. KG
    Inventors: Martin Maichl, Michael Berner
  • Patent number: 9878324
    Abstract: Fluidic devices and methods including those that provide storage and/or facilitate fluid handling of reagents are provided. Fluidic devices described herein may include channel segments positioned on two sides of an article, optionally connected by an intervening channel passing through the article. The channel segments may be used to store reagents in the device prior to first use by an end user. The stored reagents may include fluid plugs positioned in linear order so that during use, as fluids flow to a reaction site, they are delivered in a predetermined sequence. The specific geometries of the channel segments and the positions of the channel segments within the fluidic devices described herein may allow fluid reagents to be stored for extended periods of time without mixing, even during routine handling of the devices such as during shipping of the devices, and when the devices are subjected to physical shock or vibration.
    Type: Grant
    Filed: December 22, 2016
    Date of Patent: January 30, 2018
    Assignee: OPKO Diagnostics, LLC
    Inventors: Jason Taylor, Vincent Linder
  • Patent number: 9829426
    Abstract: Provided is a fluid analysis cartridge configured to analyze a fluid specimen, a fluid analysis sheet forming the same and a method of manufacturing the fluid analysis cartridge. The fluid analysis sheet includes at least one cut out part configured to form an intermediate layer of an inspection substrate, the cut out part including a flow passage structure including an inlet part in which a fluid is introduced and an inspection part in which the fluid is introduced to react with a reagent; and wherein the cut out part is further configured to include a first end adjacent to the inspection part and a second end adjacent to the inlet part, and wherein a minimum distance between the first end and the flow passage structure is at least 1 mm.
    Type: Grant
    Filed: June 18, 2015
    Date of Patent: November 28, 2017
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Seung Jun Lee, Jung Ki Min, Seung Hoon Kim, Ji Young Park, Hyun-Suk Kang
  • Patent number: 9737912
    Abstract: There is provided a microparticle sorting method including a procedure of collecting a microparticle in a fluid that flows through a main channel in a branch channel that is in communication with the main channel by generating a negative pressure in the branch channel. In the procedure, a flow of a fluid is formed that flows toward a side of the main channel from a side of the branch channel at a communication opening between the main channel and the branch channel.
    Type: Grant
    Filed: August 9, 2013
    Date of Patent: August 22, 2017
    Assignee: Sony Corporation
    Inventor: Tatsumi Ito
  • Patent number: 9649632
    Abstract: The present disclosure sets forth incorporating microfluidic chips interfaces for use with digital microfluidic processes. Methods and devices according to the present disclosure utilize compact, integrated platforms that interface with a chip upstream and downstream of the reaction, as well as between intermediate reaction steps if needed. In some embodiments these interfaces are automated, including automation of a multiple reagent process. Various reagent delivery systems and methods are also disclosed.
    Type: Grant
    Filed: June 10, 2013
    Date of Patent: May 16, 2017
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: R. Michael Van Dam, Gaurav Shah, Pei-Yuin Keng
  • Patent number: 9651489
    Abstract: An assay device includes: a liquid sample receiving zone; a capture zone in fluid communication with the sample receiving zone having capture elements bound thereto, the capture zone having a substrate and projections which extend substantially vertically from the substrate, the projections having a rhombus-shaped cross-section and the projections arranged on the substrate with the corners of the projections facing upstream in a direction toward the sample receiving zone. The projections have a height, cross-section and a distance between one another that defines a capillary space between the projections capable of generating capillary flow parallel to the substrate surface; and a sink in fluid communication with the capture zone having a capacity to receive liquid sample flowing from the capture zone. The sample receiving zone, the capture zone and the sink define a fluid flow path. Preferably, the rhombus-shaped cross-section is a square or a diamond.
    Type: Grant
    Filed: April 4, 2012
    Date of Patent: May 16, 2017
    Assignee: Ortho-Clinical Diagnostics, Inc.
    Inventors: Zhong Ding, David Bergman
  • Patent number: 9588026
    Abstract: Sample application systems can include an extraction mechanism to remove a sample from sample containers, a sample vessel disposed on a deployment mechanism, where the deployment mechanism is arranged to move the sample vessel to receive a sample, an extraction mechanism washing station to wash the extraction mechanism, a sample applicator to remove a portion of the sample in the sample vessel and apply it onto a sample carrier, where the deployment mechanism can move the sample vessel to a sample application position, a sample vessel washing station to wash the sample vessel, where the deployment mechanism can move the sample vessel to the sample vessel washing station, a sample applicator washing station to wash the sample applicator after the sample has been dispensed onto the sample carrier, and a fluid control system to control flow of a fluid provided to the extraction mechanism and the sample applicator.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: March 7, 2017
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Brian J. McKeen, Eric D. Yeaton, James L. Dowling, David Zahniser, Stephen Conroy
  • Patent number: 9575078
    Abstract: A sample testing cartridge is usable to perform a variety of tests on a visco-elastic sample, such hemostasis testing on a whole blood or blood component sample. The cartridge includes a sample processing portion that is in fluid communication with a sample retention structure. A suspension, such as a beam, arm, cantilever or similar structure supports or suspends the sample retention portion relative to the sample processing portion in a unitary structure. In this manner, the sample retention portion may be placed into dynamic excitation responsive to excitation of the cartridge and correspondingly dynamic, resonant excitation of the sample contained within the sample retention portion, while the sample processing portion remains fixed. Observation of the excited sample yields data indicative of hemostasis. The data may correspond to hemostasis parameters such as time to initial clot formation, rate of clot formation, maximum clot strength and degree of clot lysis.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: February 21, 2017
    Assignee: Coramed Technologies, LLC
    Inventors: Peter Delmenico, Carlos G. Lopez-Espina, Gabriel Raviv
  • Patent number: 9551643
    Abstract: Systems for sterile separation of magnetically labeled sample components and methods for using the same are provided. Embodiments of the systems include a magnetic separation device and a pliant sample container, where a portion of the pliant sample container is operatively coupled under pressure to the magnetic separation device. Also provided are methods of using the systems, as well as pliant sample containers configured for use with the subject systems and methods.
    Type: Grant
    Filed: November 26, 2012
    Date of Patent: January 24, 2017
    Assignee: Becton, Dickinson and Company
    Inventors: Brian David Warner, Liping Yu
  • Patent number: 9507139
    Abstract: A specimen holder is used for an optical microscope, comprising: a specimen support that supports a specimen to enable the specimen to tilt relative to the optical axis of the optical microscope; an adjustment plate that has an observation surface for making observations using the optical microscope; and an adjustment plate support that supports the adjustment plate, so that the angle formed by the optical axis and the observation surface is larger than the angle formed by the optical axis and a specimen surface of the specimen.
    Type: Grant
    Filed: November 18, 2015
    Date of Patent: November 29, 2016
    Assignee: JEOL Ltd.
    Inventors: Tsutomu Negishi, Tooru Kasai
  • Patent number: 9480981
    Abstract: An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface and an outlet for driving out the sample fluid. The device further comprises an interface assembly comprising: a third layer, a fourth layer, a fifth layer, and a flow path. The interface assembly is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer of the interface assembly and the outlet is in contact with the flow path of the interface assembly for driving out the sample fluid from the integrated device.
    Type: Grant
    Filed: July 25, 2014
    Date of Patent: November 1, 2016
    Assignee: General Electric Company
    Inventors: Ralf Lenigk, Erin Jean Finehout, Xuefeng Wang
  • Patent number: 9457497
    Abstract: A microfluidic device having a substrate with an array of curvilinear cavities. The substrate of the microfluidic device is preferably fabricated of a polymer such as polydimethylsiloxane. The microfluidic device is manufactured using a gas expansion molding technique.
    Type: Grant
    Filed: May 11, 2012
    Date of Patent: October 4, 2016
    Assignee: University of Rochester
    Inventor: Lisa A. DeLouise
  • Patent number: 9446360
    Abstract: A method and chip for fusing droplets in a chip comprising (i) providing a first set of droplets which are partially or not stabilized by surfactant, (ii) providing a second set of droplets which are stabilized by surfactant, (iii) contacting each droplet of the first set with single or multiple droplets of the second set in a pairing module and (iv) fusing droplets of the first set with droplets of the second set through a droplet fusion module in which the droplets experience at least one acceleration and/or deceleration and/or reorientation and/or rearrangement in space and time.
    Type: Grant
    Filed: May 7, 2010
    Date of Patent: September 20, 2016
    Assignees: UNIVERSITE DE STRASBOURG, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFQUE
    Inventor: Linas Mazutis
  • Patent number: 9440232
    Abstract: Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: September 13, 2016
    Assignee: Raindance Technologies, Inc.
    Inventors: Seth Fraden, Galder Cristobal-Azkarate
  • Patent number: 9409173
    Abstract: Provided herein are devices and methods for generating microfluidic gradients, including an array of unique microfluidic gradients within an array of microchannels. Fluids within conduits are mixed in an intersection region to generate a mixed flow stream in a source reservoir channel that provides a gradient that varies with axial distance from the intersection region. Microchannels having an inlet connected to the source reservoir channel are configured to provide a microfluidic gradient in the microchannel. An outlet end of the microchannel is connected to a sink reservoir channel. By varying the ratio of fluid flow rates from the fluid conduits, the microchannel gradients are tuned. In this manner, a large number of unique gradients or array of microfluidic gradients is provided, wherein the gradient can be any number of physical or chemical parameters, including concentrations and physical fluid properties.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: August 9, 2016
    Assignee: The Arizona Board of Regents on Behalf of The University of Arizona
    Inventors: Matthew D Estes, Cedric M Hurth, Frederic Zenhausern
  • Patent number: 9308601
    Abstract: A processing system for micro processing. The processing system comprises a laser configured to generate a laser beam for performing laser processing within a preparation chamber. The processing system further comprises a transmission window, configured such that the laser beam enters into the preparation chamber through the transmission window. The processing system further comprises a fastening flange for fixing the transmission window relative to the preparation chamber, and a laser shield configured to provide, in a first arrangement of the laser shield, glare protection from a passage of the laser beam from a laser beam housing to the preparation chamber. The laser shield is movably mounted for movement between the first arrangement and a second arrangement of the laser shield, wherein in the second arrangement, at least one of the transmission window and the fastening flange is separable from the processing system.
    Type: Grant
    Filed: June 19, 2013
    Date of Patent: April 12, 2016
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Volker Himmelsbach, Sebastian Alscher, Holger Doemer, Bernd Stenke, Ulrich Spittler-Herrmann
  • Patent number: 9234888
    Abstract: Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: January 12, 2016
    Assignee: OPKO Diagnostics, LLC
    Inventors: Vincent Linder, David Steinmiller, Samuel K. Sia
  • Patent number: 9182322
    Abstract: Microfluidic devices are described that include a rigid base layer, and an elastomeric layer on the base layer. The elastomeric layer may include at least part of a fluid channel for transporting a liquid reagent, and a vent channel that accepts gas diffusing through the elastomeric layer from the flow channel and vents it out of the elastomeric layer. The devices may also include a mixing chamber fluidly connected to the fluid channel, and a control channel overlapping with a deflectable membrane that defines a portion of the flow channel, where the control channel may be operable to change a rate at which the liquid reagent flows through the fluid channel. The devices may further include a rigid plastic layer on the elastomeric layer.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: November 10, 2015
    Assignee: Fluidigm Corporation
    Inventors: Tim Woudenberg, Jing Wang, Hou-Pu Chou
  • Patent number: 9101924
    Abstract: An interface device for bio-chip at least comprises an interface unit, said interface unit consisting of instrument interface layer, fluid channel layer and sample interface layer. The instrument interface layer has at least one instrument interface. The fluid channel layer has one hollow-out fluid channel. The sample interface layer has at least one sample interface, and both ends of said fluid channel connect to the sample interface and the instrument interface respectively. The interface device separates sample solutions from the instrument interface by gas or liquid in the fluid channel, thus to avoid direct contacts between sample solutions and instrument interface which will cause pollution, and omitting cleaning processes after using instruments. With simple structure, easy operation and low cost, it applies to chemistry field, biology field and medical analysis field.
    Type: Grant
    Filed: February 10, 2012
    Date of Patent: August 11, 2015
    Assignees: CAPITALBIO CORPORATION, TSINGHUA UNIVERSITY
    Inventors: Jiaming Ye, Pinhong Wang, Guoqing Wang, Wanli Xing
  • Publication number: 20150147777
    Abstract: An integrated fluidic device comprising includes an input chamber that provides an input of a sample fluid, and a first overspill chamber in fluid communication with the input chamber. A metering conduit is in fluid communication with the fluid input chamber and the first overspill chamber. The metering conduit meters a first metered volume of fluid from the sample fluid, and the first overspill chamber receives fluid in excess of the first metered volume of fluid. A second overspill chamber is in fluid communication with the metering conduit. The metering conduit meters a second metered volume of fluid from the first metered volume of fluid, and the second overspill chamber receives fluid from the first metered volume of fluid in excess of the second metered volume of fluid. The second overspill chamber has a fluid actuated closable valve for controlling the metering of the second metered volume of fluid.
    Type: Application
    Filed: November 22, 2013
    Publication date: May 28, 2015
    Applicant: SHARP KABUSHIKI KAISHA
    Inventor: Pamela Ann Dothie
  • Patent number: 9039996
    Abstract: A micro device includes a substrate and a structure configured to bind to an object or a material, or not to bind to an object or material. The structure has a roughness based on a roughness of the object or material. For example, a microarray includes a substrate and a well positioned in the substrate and configured to bind to a type of bead. The well has a roughness based on a roughness of the type of bead to which the well is configured to bind. The roughness of the well is controlled by controlling a position and number of striations in the side of the well. In another example, a moveable component of a micro device may have a roughness different from a roughness of an adjacent component, to reduce the likelihood of the moveable component sticking to the adjacent component.
    Type: Grant
    Filed: October 12, 2011
    Date of Patent: May 26, 2015
    Assignee: STMicroelectronics, Inc.
    Inventors: Massimiliano Pesaturo, Robert J. Powell
  • Patent number: 9040285
    Abstract: A sensing device including an inlet port for receiving a fluid, a measurement chamber for sensing the fluid, a fluid channel coupling the inlet port and the measurement chamber for transporting the fluid from the inlet port to the measurement chamber, and a fluid stop unit for stopping and controllably releasing the flow of fluid between the inlet port and the measurement chamber.
    Type: Grant
    Filed: December 7, 2009
    Date of Patent: May 26, 2015
    Assignee: Koninklijkle Philips N.V.
    Inventors: Albert Hendrik Jan Immink, Albert F. Gootzen, Dirk Jan Nelly Vananderoye, Petrus Johannes Wilhelmus Van Lankvelt
  • Publication number: 20150132742
    Abstract: Microfluidic devices fabricated from paper that has been covalently modified to increase its hydrophobicity, as well as methods of making and using thereof are provided herein. The devices are typically small, portable, flexible, and both easy and inexpensive to fabricate. Microfluidic devices contain a network of microfluidic components, including microfluidic channels, microfluidic chambers, microwells, or combinations thereof, designed to carry, store, mix, react, and/or analyze liquid samples. The microfluidic channels may be open channels, closed channels, or combinations thereof. The microfluidic devices may be used to detect and/or quantify an analyte, such as a small molecules, proteins, lipids polysaccharides, nucleic acids, prokaryotic cells, eukaryotic cells, particles, viruses, metal ions, and combinations thereof.
    Type: Application
    Filed: June 3, 2013
    Publication date: May 14, 2015
    Inventors: Martin Mwangi Thuo, Ramses V. Martinez, Ana C. Glavan, Wenjie Lan, Xinyu Liu, Jean-Francis Bloch, George M. Whitesides
  • Publication number: 20150132837
    Abstract: The present invention relates generally to droplet creation, fusion and sorting, and incubation for droplet-based microfluidic assays. More particularly, the present invention relates to delay-lines, which allow incubation of reactions for precise time periods. More particularly, the present invention relates to delay lines for incubations up to three hours, while reducing back-pressure and dispersion in the incubation time due to the unequal speeds with which droplets pass through the delay line.
    Type: Application
    Filed: January 27, 2015
    Publication date: May 14, 2015
    Inventors: LUCAS FRENZ, KERSTIN GODELINDE BLANK, ANDREW DAVID GRIFFITHS, ERIC BROUZES
  • Publication number: 20150132753
    Abstract: In one aspect, a microfluidic device for multiple reactions is provided, which comprises a reaction channel comprising multiple reaction chambers connected to a closed chamber or an elastic balloon outside of the microfluidic device, wherein a wall of the closed chamber is an elastic membrane; and a control channel comprising an elastic side wall, wherein the intersections between the side wall of the control channel with the reaction channel form multiple pneumatic microvalves. In another aspect, a method for conducting multiple reactions using the microfluidic device is provided, which comprises: a) filling the reaction chambers with a sample; and b) applying pressure to the control channel to expand the elastic side wall of the control channel, wherein the expanded elastic side wall forms a pneumatic microvalve that separates the reaction chambers.
    Type: Application
    Filed: May 7, 2013
    Publication date: May 14, 2015
    Inventors: Jiaming Ye, Kaihuan Zhang, Wanli Xing, Jing Cheng
  • Patent number: 9028776
    Abstract: The invention provides structures and methods that allow polymers of any length, including nucleic acids, to be stretched into a long, linear conformation for further analysis.
    Type: Grant
    Filed: April 12, 2013
    Date of Patent: May 12, 2015
    Assignee: Toxic Report LLC
    Inventors: Robert H. Meltzer, Joshua W. Griffis
  • Patent number: 9028775
    Abstract: This technology is a method and apparatus for the semi-continuous measurement of the concentration of constituents of airborne particles which couples a laminar flow, water condensation particle collector to a microfluidic device for assay of particle chemical composition by electrophoresis. The technology has been used for the assay of sulfates, nitrates, chlorides, and organic acids contained in fine and submicrometer atmospheric particles. For these compounds the apparatus and method described is capable of one-minute time resolution at concentrations at the level of micrograms of analyte species per cubic meter of air. Extension to other analytes is possible.
    Type: Grant
    Filed: September 27, 2011
    Date of Patent: May 12, 2015
    Assignees: Aerosol Dynamics Inc., Colorado State University Research
    Inventors: Susanne Vera Hering, Gregory Stephen Lewis, Steven Russel Spielman, Charles Sherman Henry, Scott Douglas Noblitt, Jeffrey Lee Collett, Jr.
  • Patent number: 9029158
    Abstract: Described herein are particular embodiments relating to a microfluidic device that may be utilized for cell sensing, counting, and/or sorting. Particular aspects relate to a microfabricated device that is capable of differentiating single cell types from dense cell populations. One particular embodiment relates a device and methods of using the same for sensing, counting, and/or sorting leukocytes from whole, undiluted blood samples.
    Type: Grant
    Filed: June 4, 2014
    Date of Patent: May 12, 2015
    Assignee: California Institute of Technology
    Inventors: Yu-Chong Tai, Siyang Zheng, Jeffrey Chun-Hui Lin, Harvey Kasdan
  • Publication number: 20150125947
    Abstract: The invention provides a new microfluidic device and method for performing operations on droplets. The invention extends to microfluidic systems comprising one or more of the microfluidic devices.
    Type: Application
    Filed: April 25, 2013
    Publication date: May 7, 2015
    Applicant: Scope Fluidics SP Z O.O.
    Inventors: Piotr Korczyk, Ladislav Derzsi, Tomasz Kaminski, Slawomir Jakiela, Piotr Garstecki
  • Publication number: 20150125882
    Abstract: The present disclosure provides methods and systems for assaying a sample. A microfluidic device to perform an assay of a sample (e.g., biological sample) is described having a sample application site, a porous component and a flow channel. The porous component provides for uniform dissolution of a reagent and mixing of the sample and reagent without filtering the sample.
    Type: Application
    Filed: November 5, 2014
    Publication date: May 7, 2015
    Inventors: Scott Joseph Bornheimer, Jeffrey Sugarman, Wei Huang, Edward Michael Goldberg, Ming Tan
  • Publication number: 20150125879
    Abstract: Compositions and methods for providing devices comprised of a substrate and a Layer-By-Layer (LBL) film coated on at least a surface of the substrate, which LBL film comprises binding agents that specifically interact with cells. Such devices are useful, for example, in various cell isolation applications. Among other advantages, such devices permit isolation and release (e.g., via layer degradation) of cells under mild conditions.
    Type: Application
    Filed: September 25, 2014
    Publication date: May 7, 2015
    Inventors: Wei Li, Steven Andrew Castleberry, Paula Therese Hammond
  • Patent number: 9023294
    Abstract: Provided is a cell concentration and purification device, having: a function of continuously concentrating cells; a function of then subsequently disposing the cells continuously in a specific region of a channel; a function of simultaneously recognizing, based on an image, the shape and fluorescence emission of each single cell; and a function of recognizing the cells and then separating and purifying the same based on the data relating to the shape and fluorescence emission thereof.
    Type: Grant
    Filed: February 24, 2011
    Date of Patent: May 5, 2015
    Assignees: Kanagawa Academy of Science and Technology, National University Corporation Tokyo Medical and Dental University, On-Chip Cellonics Consortium Co., Ltd.
    Inventors: Hideyuki Terazono, Kenji Yasuda, Masahito Hayashi, Hiroyuki Takei, Akihiro Hattori
  • Patent number: 9023641
    Abstract: The present invention relates to an leucocyte antigen mediated microfluidic assay and a microfluidic device for analyzing a subjects' body fluids containing leucocytes to determine if the subject has been previously exposed to a predetermined antigen.
    Type: Grant
    Filed: May 13, 2005
    Date of Patent: May 5, 2015
    Assignee: Advanced Animal Diagnostics
    Inventors: Rodolfo R. Rodriguez, Douglas J. Darr
  • Patent number: 9023279
    Abstract: A self-contained assay facility housed in a fixed-wing or rotary wing aircraft that is completely equipped to melt and assay precious metals, particularly gold and silver. An induction furnace melts the metal that is then poured into an ingot. The ingot is weighed and analyzed using an XRF alloy analyzer and the percentage of gold and/or other metals recorded. The value of the gold at current market prices is calculated and the assay and the value of the ingot is printed and given to the seller. The seller may opt to receive the ingot and pay the assayer an assay fee. Alternately, the seller may ask to be paid in cash, in bullion, by wire transfer, or by an open hedge. A transfer or hedge is initiated and confirmed from the assay facility. The ingots are securely stored in a safe within the assay facility.
    Type: Grant
    Filed: April 16, 2013
    Date of Patent: May 5, 2015
    Assignee: AOW Holdings, LLC
    Inventor: Chris M. Noyes
  • Patent number: 9023281
    Abstract: Chemical indicator apparatuses containing one or more chemical indicators for use in monitoring the quality of water in an aquatic environment. The apparatuses are designed and configured to be submersible in the water that is being monitored. In some embodiments, each apparatus includes a plurality of immobilized-dye-based chemical indicators that undergo a physical change as levels of one or more constituents of the water change. Such indicators can be read by one or more suitable optical readers. These and other embodiments are designed and configured to be movable by a corresponding monitoring/measuring apparatus, for example, via a magnetically coupled drive. Also disclosed are a variety of features that can be used to provide a chemical indicator apparatus with additional functionalities.
    Type: Grant
    Filed: December 13, 2012
    Date of Patent: May 5, 2015
    Assignee: Step Ahead Innovations, Inc.
    Inventor: James E. Clark
  • Patent number: 9023296
    Abstract: A method of manufacturing a target reactor having a flow-channel system in which a plurality of reactants continuously flowing into said target reactor are mixed and interconvert to form a target volumetric flow-rate (f2) of a product continuously flowing out of said target reactor, wherein the smallest hydraulic diameter (dh2) of said target reactor is calculated based on the relationship d h ? ? 2 = d h ? ? 1 ? ( f 2 f 1 ) 3 - n 7 - n in a turbulent or transitional turbulent flow, wherein n is a non-integer number with 1>n?0, between the corresponding smallest hydraulic diameter (dh1) of a standard reactor having the same fluidic type of flow-channel system, f1 is a standard volumetric flow-rate of said standard reactor carrying out the same interconversion, and f2 is said target volumetric flow-rate.
    Type: Grant
    Filed: May 12, 2010
    Date of Patent: May 5, 2015
    Assignee: Lonza AG
    Inventors: Dominique Roberge, Norbert Kockmann, Michael Gottsponer, Markus Eyholzer