Micro-fluidic Device Patents (Class 422/502)
  • Patent number: 11135587
    Abstract: There is provided a particle supplementing chip provided with a structure for trapping a particle, and for preventing the trapped particle from being greatly deformed by a suction force. With respect to this point, the present technology provides a particle trapping chip including a first channel, a second channel, a first recess that is open on the first channel side, a second recess that is provided side by side with the first recess, a connecting portion connecting the first recess and the second recess, and a communicating portion allowing the second recess and the second channel to communicate with each other.
    Type: Grant
    Filed: November 21, 2017
    Date of Patent: October 5, 2021
    Assignee: SONY CORPORATION
    Inventors: Kensuke Kojima, Shin Masuhara
  • Patent number: 11097267
    Abstract: A microfluidic device includes at least one substrate formed of one or more silicon wafers. The substrate includes an inlet for receiving a continuous phase fluid; an inlet for receiving a dispersed phase fluid; and a plurality of channels. The plurality of channels are in fluid communication with both the inlet of the continuous phase fluid and the inlet of the dispersed phase fluid. The substrate further includes a plurality of droplet generators configured to produce microdroplets. Each of the droplet generators are in fluid communication with the plurality of channels. Additionally, the substrate includes one or more outlets for delivery of the microdroplets. The number of the plurality of droplet generators is more than two greater than a number of the one or more outlets for delivery of the microdroplets.
    Type: Grant
    Filed: December 14, 2016
    Date of Patent: August 24, 2021
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Sagar Yadavali, David Issadore, Daeyeon Lee
  • Patent number: 11096388
    Abstract: A perfusion bioreactor and a perfusion device. Each perfusion device has a mesh structure, and an encapsulated organ tissue (EOT) disposed in the mesh structure. The EOT has a body with a thickness defined between a first surface of the body and a second surface of the body. The body has at least one channel extending into the body from one of the first and second surfaces to receive a fluid therein. The at least one channel has a diameter selected to diffuse solutes out of the fluid and into the body. The perfusion devices are arranged one adjacent to another and spaced apart from each other along the length of the bioreactor to receive fluid, and to perfuse the fluid to the EOT of each perfusion device and to the at least one channel therein. A method of processing blood plasma and an artificial liver system are also disclosed.
    Type: Grant
    Filed: May 22, 2019
    Date of Patent: August 24, 2021
    Assignee: MORPHOCELL TECHNOLOGIES INC.
    Inventors: Radu Alexandru Paun, Shawana Habib, Massimiliano Paganelli, Claudia Raggi
  • Patent number: 11078523
    Abstract: A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.
    Type: Grant
    Filed: December 7, 2020
    Date of Patent: August 3, 2021
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Gene Parunak, Aaron Kehrer, Betty Wu, Karthik Ganesan
  • Patent number: 11071979
    Abstract: A microchip includes a plurality of laminated elastic sheets. Each of the elastic sheets forming a first intermediate layer as an intermediate layer formed with the plurality of elastic sheets have an inadhesive section(s) for forming a first flow path on the first intermediate layer. Each of the elastic sheets for forming a second intermediate layer as an intermediate layer formed with the plurality of elastic sheets have an inadhesive section(s) for forming a second flow path on the second intermediate layer. An elastic sheet(s) interposed between the first and second intermediate layers has a connecting section(s) connecting the first flow path and the second flow path. A flow path width at the connecting section(s) of the first flow path is narrower than a flow path width at the connecting section(s) of the second flow path.
    Type: Grant
    Filed: December 14, 2015
    Date of Patent: July 27, 2021
    Assignee: NEC CORPORATION
    Inventors: Minoru Asogawa, Yasuo Iimura
  • Patent number: 11041185
    Abstract: A system for testing a treatment agent for a biologic material includes an input for receiving a biologic sample. A plurality of micro-pumps pump a portion of the biologic sample from the first reservoir into a connected module. A first module includes a first plurality of testing pathways for testing a first portion of the biologic sample. A first module connector removeably connects the first module to the distributor module. A second module includes a second plurality of testing pathways for testing a second portion of the biologic sample. The selected pathway applies at least one dosage level of a treatment agent to the second portion of the biologic sample. A second module connector removeably connects the second module to the distributor module, wherein treatment agent and the plurality of dosage levels tested by the system may be selected by selecting the second module associated with the second module connector.
    Type: Grant
    Filed: November 10, 2018
    Date of Patent: June 22, 2021
    Assignee: RELIANT IMMUNE DIAGNOSTICS, INC.
    Inventors: Jovan Hutton Pulitzer, Henry Joseph Legere, III
  • Patent number: 11035780
    Abstract: [Problems] Objects include providing a cover film for testing which can be well fixed to a substrate having a groove and in which a pressure sensitive adhesive does not invade into the groove, providing a testing member comprising the cover film for testing, and providing a method of manufacturing the cover film for testing. [Solution] The cover film for testing (1, 2) comprises a base material (10) and a pressure sensitive adhesive layer (20) laminated on one surface side of the base material (10). The cover film for testing (1, 2) has a region in which the pressure sensitive adhesive layer (20) does not exist in the plan view.
    Type: Grant
    Filed: May 19, 2020
    Date of Patent: June 15, 2021
    Assignee: LINTEC CORPORATION
    Inventors: Masaya Todaka, Hiroki Hoshino, Tomoo Orui
  • Patent number: 10962431
    Abstract: A pressure sensor designed to detect a value of ambient pressure of the environment external to the pressure sensor includes: a first substrate having a buried cavity and a membrane suspended over the buried cavity; a second substrate having a recess, hermetically coupled to the first substrate so that the recess defines a sealed cavity the internal pressure value of which provides a pressure-reference value; and a channel formed at least in part in the first substrate and configured to arrange the buried cavity in communication with the environment external to the pressure sensor. The membrane undergoes deflection as a function of a difference of pressure between the pressure-reference value in the sealed cavity and the ambient-pressure value in the buried cavity.
    Type: Grant
    Filed: December 14, 2018
    Date of Patent: March 30, 2021
    Assignee: STMICROELECTRONICS S.r.l.
    Inventors: Enri Duqi, Sebastiano Conti, Sonia Costantini
  • Patent number: 10953399
    Abstract: An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.
    Type: Grant
    Filed: January 10, 2019
    Date of Patent: March 23, 2021
    Assignees: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITED
    Inventors: Wesley A. Cox-Muranami, Darren Robert Segale, Oliver Jon Miller, Jennifer Olivia Foley, Tarun Kumar Khurana, Paul Crivelli, Gary Watts
  • Patent number: 10946384
    Abstract: A thermal convection generating device (1) that generates thermal convection of a liquid by heating or cooling the liquid and applying centrifugal force to the liquid, the thermal convection generating device including: a stage (20) to which a thermal convection generating chip (10) is attachable and detachable, the thermal convection generating chip including a disk-like substrate (11) and a thermal convection pathway (14) formed in a surface of the disk-like substrate that is perpendicular to an axis (AX) of the disk-like substrate, and being configured to rotate about the axis of the disk-like substrate to apply centrifugal force to a liquid in the thermal convection pathway; a heat controller (30) that heats or cools a portion of the thermal convection pathway; a motor (40) that drives the thermal convection generating chip to rotate about the axis; and a controller (50) that controls the heat controller and the motor.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: March 16, 2021
    Assignee: OSAKA UNIVERSITY
    Inventors: Masato Saito, Eiichi Tamiya
  • Patent number: 10946377
    Abstract: A modular analytic system includes a base, at least one fluid sample processing module configured to be removably attached to the base, at least one fluid sample analysis module configured to be removably attached to the base, a fluid actuation module positioned on the base, a fluidic network comprising multiple fluidic channels, in which the fluid actuation module is arranged to control transport of a fluid sample between the at least one sample processing module and the at least one sample analysis module through the fluidic network, and an electronic processor, in which the electronic processor is configured to control operation of the fluid actuation module and receive measurement data from the at least one fluid sample analysis module.
    Type: Grant
    Filed: February 1, 2019
    Date of Patent: March 16, 2021
    Assignee: The General Hospital Corporation
    Inventors: Robert Granier, Ramin Haghgooie, Ken Kotz, Anne C. Petrofsky, Ronald G. Tompkins
  • Patent number: 10940474
    Abstract: A microfluidic device for measuring an amount of an oxidant in a solution is disclosed. The device includes a microfluidic substrate configured to mix a solution sample to be analysed with an indicator dye solution containing an indicator dye under conditions suitable for some of the indicator dye to react with any oxidant in the solution to produce an oxidant measurement solution having a reduced indicator dye concentration that is indicative of the amount of oxidant in the solution, the microfluidic substrate including an optical reading window through which the reduced indicator dye concentration in the oxidant measurement solution can be measured optically.
    Type: Grant
    Filed: March 20, 2017
    Date of Patent: March 9, 2021
    Assignee: University of South Australia
    Inventors: Thomas Nann, Luke Andrew Parkinson, Sait Elmas, Craig Ian Priest, Vasil Rosenov Vasilev
  • Patent number: 10930466
    Abstract: An ion milling apparatus includes an ion irradiation source, a sample holder, a sample stage, a rotation mechanism, and a slide mechanism. The sample holder holds a sample such that the sample protrudes from a shielding plate in a direction perpendicular to an optical axis of an ion beam. The rotation mechanism is disposed such that a rotation center of a rotation shaft is perpendicular to the optical axis of the ion beam and parallel to a direction in which the sample protrudes from the shielding plate. The rotation mechanism supports the sample stage such that the sample stage is rotatable. The slide mechanism supports the sample held by the sample holder such that the sample is movable along the optical axis of the ion beam.
    Type: Grant
    Filed: May 24, 2018
    Date of Patent: February 23, 2021
    Assignee: JEOL Ltd.
    Inventors: Shogo Kataoka, Shunsuke Asahina
  • Patent number: 10829728
    Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.
    Type: Grant
    Filed: June 15, 2018
    Date of Patent: November 10, 2020
    Assignee: Berkeley Lights, Inc.
    Inventors: Volker L. S. Kurz, Troy A. Lionberger, Eric K. Sackmann, Kai W. Szeto, Paul M. Lebel, Brandon R. Bruhn, Keith J. Breinlinger, Eric D. Hobbs, Andrew W. McFarland, J. Tanner Nevill, Xiaohua Wang
  • Patent number: 10807092
    Abstract: The present disclosure relates to a microfluidic control system and a microfluidic control method using the same. The microfluidic control system includes: a microfluidic chip including a storage chamber for storing a reaction solution and a receiving chamber communicating with the storage chamber; and a microfluidic control device for controlling the reaction solution inside the microfluidic chip, wherein the microfluidic control device includes: a first roller which is in contact with the microfluidic chip and rotates together with the movement of the microfluidic chip; and a pressurizing protrusion formed on the outer peripheral surface of the first roller, wherein the pressurizing protrusion has a shape corresponding to the storage chamber.
    Type: Grant
    Filed: February 23, 2018
    Date of Patent: October 20, 2020
    Assignee: ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE
    Inventors: Kwang Hyo Chung, Jin Tae Kim, Eun-Ju Jeong, Bong Kyu Kim, Chang-Geun Ahn
  • Patent number: 10746733
    Abstract: A microfluidic device for conducting a fluid assay includes an injection-molded (or “molded”) fluidics layer having at least one microfluidic channel configured to allow assay fluids to flow there-along, the channel having channel side walls, a channel bottom, and a channel 3D geometry, and the fluidics layer being made from injection-molded liquid silicone (or PDMS). Having the fluidics layer made from injection molded liquid silicone enables smaller-sized channel features, such as microfluidic valves and pistons, smaller channel dimensions and spacing (providing smaller device footprint, higher device capacity and other benefits), and various geometries for the channels and channel features.
    Type: Grant
    Filed: March 8, 2019
    Date of Patent: August 18, 2020
    Assignee: PROTEINSIMPLE
    Inventors: Charles Stanwood, Noel Daigneault, Jeffrey Branciforte
  • Patent number: 10737266
    Abstract: In one embodiment, a removable pneumatic connector, comprises a body having a plurality of bores passing through, each bore surrounded by a sealing member on an inner surface of the body. A plurality of gas lines may be placed within a corresponding bore. A vacuum port is disposed on the inner surface of the body, and an outer seal on the inner surface of the body surrounds the sealing members and the vacuum port. A vacuum line may be placed within the vacuum port, and configured to deliver negative pressure to the vacuum port. A vacuum holding area is created in the volume between the outer seal and each of the sealing members when the inner surface of the body is placed against a substrate. When the vacuum line is activated, a vacuum is created within the vacuum holding area, creating a positive seal between the body and the substrate.
    Type: Grant
    Filed: March 13, 2019
    Date of Patent: August 11, 2020
    Assignee: EMD Millipore Corporation
    Inventors: Terry Gaige, Andrew Zayac, Paul Sydlowski, Philip Lee
  • Patent number: 10722880
    Abstract: Disclosed herein are methods, devices, and systems for efficient loading and retrieval of particles. In some embodiments, a fluidic channel of a flowcell comprises a ceiling, a first sidewall, and a bottom, wherein the contact angle of the ceiling is at least 10 degrees smaller than the contact angle of the first sidewall, and wherein the bottom of the fluidic channel comprises a substrate that comprises a plurality of microwells.
    Type: Grant
    Filed: January 11, 2018
    Date of Patent: July 28, 2020
    Assignee: Cellular Research, Inc.
    Inventors: Philipp Spuhler, Sixing Li
  • Patent number: 10710083
    Abstract: The present invention relates to a microfluidic system including a temperature controller and a thermoplastic microfluidic chip that enables rapid PCR in a PCR chamber of the microfluidic chip. Thermal control of the PCR chamber is achieved by applying voltage to heater electrodes patterned directly onto one layer of the microfluidic chip. The temperature controller adjusts the voltage applied to the heater electrodes by changing temperature controller parameters selected to minimize duration of each PCR cycle. Furthermore, simple operation of the microfluidic chip is provided through using an integrated passive capillary valve, requiring minimum operator intervention and eliminating the need for fluidic interfacing, pumping, or metering during chip loading.
    Type: Grant
    Filed: April 14, 2017
    Date of Patent: July 14, 2020
    Assignee: University of Maryland
    Inventors: Donald DeVoe, Eric Kendall, Alex Sposito
  • Patent number: 10704537
    Abstract: A soft robot device includes at least a first thermoplastic layer and a second thermoplastic layer, wherein at least one layer is comprised of an extensible thermoplastic material; at least one layer is an inextensible layer; and at least one layer comprises a pneumatic network, wherein the pneumatic network is configured to be in fluidic contact with a pressurizing source, wherein the first and second thermoplastic layers are thermally bonded to each other.
    Type: Grant
    Filed: May 29, 2018
    Date of Patent: July 7, 2020
    Assignee: President and Fellows of Harvard College
    Inventors: Jason Ming Ting, Alok Suryavamsee Tayi, Bobak Mosadegh, George M. Whitesides
  • Patent number: 10682622
    Abstract: A micro-fluidic reactor may comprise a photosensitive glass substrate with a plurality of features produced by etching. The features may include micro-channels, micro-lenses, and slots for receiving optical fibers. During operation of the micro-fluidic reactor, the optical fibers may transmit optical signals for measuring characteristics of fluid reagents and reactions taking place. The micro-lenses may focus optical signals from the optical fibers to create an approximately collimated optical path for the optical signals, reducing optical spread and enhancing fiber-to-fiber optical power coupling.
    Type: Grant
    Filed: May 3, 2017
    Date of Patent: June 16, 2020
    Assignee: Board of Trustees of the University of Alabama, for and on behalf of the University of Alabama in Huntsville
    Inventors: William Randall Gaillard, John D. Williams
  • Patent number: 10683952
    Abstract: A method is provided involving an additive manufacturing system. This method includes a step of forming a first fluid conduit using the additive manufacturing system. The method also includes a step of providing a fluid coupling. The fluid coupling includes the first fluid conduit and a second fluid conduit. The first fluid conduit is connected to and fluidly coupled with the second fluid conduit. The first fluid conduit has a first configuration. The second fluid conduit has a second configuration that is different than the first configuration.
    Type: Grant
    Filed: November 29, 2017
    Date of Patent: June 16, 2020
    Assignee: Raytheon Technologies Corporation
    Inventors: Joe Ott, Dennis M. Moura, Stanley J. Funk, Shawn Stempinski, Roger O. Coffey, John J. Rup, Jr., Gary A. Schirtzinger, Lexia Kironn
  • Patent number: 10675620
    Abstract: The present invention is notably directed to method of fabrication of a microfluidic chip, comprising: providing a substrate, a face of which is covered by an electrically insulating layer; obtaining a resist layer covering one or more selected portions of the electrically insulating layer, at least a remaining portion of said electrically insulating layer not being covered by the resist layer; partially etching with a wet etchant a surface of the remaining portion of the electrically insulating layer to create a recess and/or an undercut under the resist layer; depositing the electrically conductive layer on the etched surface, such that the electrically conductive layer reaches the created recess and/or undercut; and removing the resist layer to expose a portion of the electrically insulating layer adjoining a contiguous portion of the electrically conductive layer. The present invention is further directed to microfluidic chips obtainable by such methods.
    Type: Grant
    Filed: December 24, 2018
    Date of Patent: June 9, 2020
    Assignee: International Business Machines Corporation
    Inventors: Emmanuel Delamarche, Tobias Guenzler, Yuksel Temiz, Tino Treiber
  • Patent number: 10632688
    Abstract: A method for fabricating flexible microfluidic chips with plastic membranes. In particular, the present invention provides a single-step method for microchannel fabrication of microfluidic chips in a fast and cost-efficient manner.
    Type: Grant
    Filed: February 14, 2018
    Date of Patent: April 28, 2020
    Assignees: Hong Kong Baptist University, THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY
    Inventors: Kangning Ren, Dik-Lung Ma, Chong Hu, Wanbo Li, Sheng Lin, Hongkai Wu
  • Patent number: 10589265
    Abstract: Fluidic devices and methods including those that provide storage and/or facilitate fluid handling of reagents are provided. Fluidic devices described herein may include channel segments positioned on two sides of an article, optionally connected by an intervening channel passing through the article. The channel segments may be used to store reagents in the device prior to first use by an end user. The stored reagents may include fluid plugs positioned in linear order so that during use, as fluids flow to a reaction site, they are delivered in a predetermined sequence. The specific geometries of the channel segments and the positions of the channel segments within the fluidic devices described herein may allow fluid reagents to be stored for extended periods of time without mixing, even during routine handling of the devices such as during shipping of the devices, and when the devices are subjected to physical shock or vibration.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: March 17, 2020
    Assignee: OPKO Diagnostics, LLC
    Inventors: Jason Taylor, Vincent Linder
  • Patent number: 10571935
    Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected in the preferred structure of the provided methods and systems. In particular, the methods are structured into low-level device component control functions, middle-level actuator control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring, mixing, heating, and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations.
    Type: Grant
    Filed: November 21, 2014
    Date of Patent: February 25, 2020
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Karthik Ganesan, Sundaresh N. Brahmasandra
  • Patent number: 10512912
    Abstract: A microfluidic system for analyzing a sample solution includes a division chamber for accommodating an input volume of the sample solution. The division chamber has a plurality of partial volume segments for accommodating a plurality of partial volumes of the sample solution, which partial volumes can be used for detection reactions. The microfluidic system also has a displacing device configured to divide the input volume into the plurality of partial volumes.
    Type: Grant
    Filed: September 11, 2015
    Date of Patent: December 24, 2019
    Assignee: Robert Bosch GmbH
    Inventors: Thomas Brettschneider, Jochen Hoffmann
  • Patent number: 10507465
    Abstract: A microfluidic probe includes a probe head with a processing surface that includes a first aperture and a second aperture. The probe further includes a liquid injection channel, which leads to the first aperture, and a liquid aspiration channel, which extends from the second aperture. The probe also includes a bypass channel, arranged so as to fluidly connect the liquid injection channel to the liquid aspiration channel, as well as a control channel. The latter fluidly connects to the bypass channel, hence forming a junction therewith, so as to define two portions of the bypass channel. These portions includes: a first portion that extends from the junction to the liquid injection channel; and a second portion that extends from that same junction to the liquid aspiration channel. The invention is further directed to methods of operation of a probe as described above, to process a surface.
    Type: Grant
    Filed: December 28, 2017
    Date of Patent: December 17, 2019
    Assignee: International Business Machines Corporation
    Inventors: Govind Kaigala, Robert Dean Lovchik, David Taylor
  • Patent number: 10444244
    Abstract: A microfluidic array supporting a lipid bilayer assembly on which membrane proteins can be assembled is described. The array is formed from a hydrophilic polymeric substrate or metal substrate comprising a planar surface with a plurality of individual spherical depressions formed therein. Each of the depressions are configured to have a diameter greater than 1 ?m and containing an aqueous solution. Across each of the depressions is provided a lipid layer. Each of the plurality of individual depressions comprise arcuate side walls extending downwardly into the substrate from the planar surface.
    Type: Grant
    Filed: July 2, 2015
    Date of Patent: October 15, 2019
    Assignee: DUBLIN CITY UNIVERSITY
    Inventors: Tia Keyes, Robert Forster, Hajra Basit, Sean Maher, Vincent Gaul
  • Patent number: 10427150
    Abstract: A pipetting apparatus for aspirating and dispensing liquids having a pipetting unit with an interchangeable vessel that receives a liquid to be transferred, a holder for the pipetting unit, a positioning device for the pipetting unit, a micrometering unit, and an electronic control device. The micrometering unit is embodied separately from the pipetting unit and has a separate electronic control unit. The pipetting unit has a coupling device and the micrometering unit has a counterpart coupling device that matches the coupling device for the purpose of coupling. Coupling device and/or counterpart coupling device is/are switchable. The holder or the micrometering unit has a contact device with at least one electrical contact element and the micrometering unit or the holder has a counterpart contact device, matching the contact device, with at least one counterpart electrical contact element for making electrical contact with the contact element.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: October 1, 2019
    Assignee: BRAND GMBH + CO KG
    Inventors: Jürgen Schraut, Steffen Gehrig
  • Patent number: 10350599
    Abstract: A microfluidic exosome profiling platform integrating exosome isolation and targeted proteomic analysis is disclosed. This platform is capable of quantitative exosomal biomarker profiling directly from plasma samples with markedly enhanced sensitivity and specificity. Identification of distinct subpopulation of patient-derived exosomes is demonstrated by probing surface proteins and multiparameter analyzes of intravesicular biomarkers in the selected subpopulation. The expression of IGF-1R and its phosphorylation level in non-small cell lung cancer (NSCLC) patient plasma is assessed as a non-invasive alternative to the conventional biopsy and immunohistochemistry. Detection of ovarian cancer also is assessed. The microfluidic chip, which may be fabricated of a glass substrate and a layer of poly(dimethylsiloxane), includes a serpentine microchannel to mix a fluid and a microchamber for the collection and detection of exosomes.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: July 16, 2019
    Assignees: University of Kansas, Kansas State University Research Foundation
    Inventors: Zheng Zhao, Mei He, Yong Zeng
  • Patent number: 10350594
    Abstract: A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.
    Type: Grant
    Filed: March 5, 2018
    Date of Patent: July 16, 2019
    Assignee: Berkeley Lights, Inc.
    Inventors: Eric D. Hobbs, Justin K. Valley
  • Patent number: 10345205
    Abstract: Sample application systems can include an extraction mechanism to remove a sample from sample containers, a sample vessel disposed on a deployment mechanism, where the deployment mechanism is arranged to move the sample vessel to receive a sample, an extraction mechanism washing station to wash the extraction mechanism, a sample applicator to remove a portion of the sample in the sample vessel and apply it onto a sample carrier, where the deployment mechanism can move the sample vessel to a sample application position, a sample vessel washing station to wash the sample vessel, where the deployment mechanism can move the sample vessel to the sample vessel washing station, a sample applicator washing station to wash the sample applicator after the sample has been dispensed onto the sample carrier, and a fluid control system to control flow of a fluid provided to the extraction mechanism and the sample applicator.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: July 9, 2019
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Brian J. McKeen, Eric D. Yeaton, James L. Dowling, David Zahniser, Stephen Conroy
  • Patent number: 10330919
    Abstract: A microfluidic system includes an electro-wetting on dielectric (EWOD) device and a control system that controls actuation voltages applied to the element array of the EWOD device to perform manipulation operations as to fluid droplets. The control system applies a sequence of actuation voltages to a portion of the array elements associated with a droplet to maintain the droplet in a desired droplet state corresponding to a predetermined droplet property. The sequence of actuation voltages includes an actuation-on period in which the portion of the array elements associated with the droplet is actuated and an actuation-off period in which the portion of the array elements associated with the droplet is not actuated, and the actuation-off period is non-zero. The control system may apply a sequence of actuation voltages comprising a predetermined duty cycle, and/or the actuation voltages may be applied in accordance with a sensor based intervention.
    Type: Grant
    Filed: March 31, 2017
    Date of Patent: June 25, 2019
    Assignee: Sharp Life Science (EU) Limited
    Inventors: Laura Huang, Tim Boyle
  • Patent number: 10295493
    Abstract: A sampling plate (1) for use in measuring a property of a fluid sample comprising a sample zone comprising at least two discrete testing zones (5, 6) for receiving different respective volumes of the fluid sample. Each testing zone presents a zone volume defined by a respective zone area and a different respective zone height being a dimension transverse to the zone area (A).
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: May 21, 2019
    Assignees: BRYTECH DESIGN & ENGINEERING PROJECT MANAGEMENT LIMITED, TAPE SPECIALITIES LIMITED
    Inventors: Matthew Bryan, Stuart Seagrave
  • Patent number: 10226767
    Abstract: A technique relates to a fluidic cell configured to hold a nanofluidic chip. A first plate is configured to hold the nanofluidic chip. A second plate is configured to fit on top of the first plate, such that the nanofluidic chip is held in place. The second plate has at least one first port and at least one second port. The second plate has an entrance hole configured to communicate with an inlet hole of the nanofluidic chip. The second port is angled above the first port, such that the first port and second port intersect to form a junction. The second port is formed to have a line-of-sight to the entrance hole, such that the second port is configured to receive input for extracting air trapped at a vicinity of the entrance hole.
    Type: Grant
    Filed: February 2, 2018
    Date of Patent: March 12, 2019
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Michael A. Pereira, Joshua T. Smith, Benjamin H. Wunsch
  • Patent number: 10196755
    Abstract: A gas phase nanowire growth apparatus including a reaction chamber, a first input and a second input. The first input is located concentrically within the second input and the first and second input are configured such that a second input fluid delivered from the second input provides a sheath between a first fluid delivered from the first input and a wall of the reaction chamber. An aerosol of catalyst particles may be used to grow the nanowires.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: February 5, 2019
    Assignee: SOL VOLTAICS AB
    Inventors: Greg Alcott, Martin Magnusson, Olivier Postel, Knut Deppert, Lars Samuelson, Jonas Ohlsson
  • Patent number: 10159976
    Abstract: The present invention is notably directed to method of fabrication of a microfluidic chip (1), comprising: providing (S1-S7) a substrate (10), a face (F) of which is covered by an electrically insulating layer (30); obtaining (S8) a resist layer (40) covering one or more selected portions (P1) of the electrically insulating layer (30), at least a remaining portion (P2) of said electrically insulating layer (30) not being covered by the resist layer; partially etching (S9) with a wet etchant (E) a surface of the remaining portion (P2) of the electrically insulating layer (30) to create a recess (40r) and/or an undercut (40u) under the resist layer (40); depositing (S10) the electrically conductive layer (50) on the etched surface (35), such that the electrically conductive layer reaches the created recess (40r) and/or undercut (40u); and removing (S11) the resist layer (40) to expose a portion (P1) of the electrically insulating layer adjoining a contiguous portion (P2) of the electrically conductive layer (50
    Type: Grant
    Filed: June 18, 2014
    Date of Patent: December 25, 2018
    Assignee: International Business Machines Corporation
    Inventors: Emmanuel Delamarche, Tobias Guenzler, Yuksel Temiz, Tino Treiber
  • Patent number: 10058869
    Abstract: A micro-fluidic device includes a plurality of heating elements on a substrate for heating the substrate. They define a plurality of temperature regions having distinct temperatures on the substrate. A flow feature layer is formed above the substrate to define a channel extending across the substrate through each temperature region. As fluid is repeatedly pumped within the channel, it flows from one temperature region to a next temperature region to undergo thermal cycling. Fluid wells reside in the flow feature layer under a cover and connect to the channel whereby fluid can dwell for diagnostic applications.
    Type: Grant
    Filed: March 21, 2016
    Date of Patent: August 28, 2018
    Assignee: Microfluidic MEMS Solution, LLC
    Inventors: Steven Wayne Bergstedt, Stephen Jacob DeMoor, Yimin Guan
  • Patent number: 10052607
    Abstract: An instrument and method for accelerating the solid phase synthesis of peptides are disclosed. The method includes the steps of deprotecting a protected first amino acid linked to a solid phase resin by admixing the protected linked acid with a deprotecting solution in a microwave transparent vessel while irradiating the admixed acid and solution with microwaves, activating a second amino acid, coupling the second amino acid to the first acid while irradiating the composition in the same vessel with microwaves, and cleaving the linked peptide from the solid phase resin by admixing the linked peptide with a cleaving composition in the same vessel while irradiating the composition with microwaves.
    Type: Grant
    Filed: June 2, 2017
    Date of Patent: August 21, 2018
    Assignee: CEM Corporation
    Inventors: Jonathan McKinnon Collins, Joseph Joshua Lambert, Michael John Collins
  • Patent number: 10024852
    Abstract: A reusable coating for a nanopore structure is disclosed herein. A nanopore structure includes a substrate comprising a nanochannel and a monolayer of a chemical compound disposed onto at least a portion of a surface of the nanochannel. The chemical compound forms a reversible bond with at least one analyte binding compound introduced into the nanochannel. Methods for making and using the reusable coating are also disclosed.
    Type: Grant
    Filed: November 4, 2013
    Date of Patent: July 17, 2018
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Natalie Gunn, Jose M. Lobez Comeras, Priscilla Rogers, John Wagner
  • Patent number: 10024851
    Abstract: A reusable coating for a nanopore structure is disclosed herein. A nanopore structure includes a substrate comprising a nanochannel and a monolayer of a chemical compound disposed onto at least a portion of a surface of the nanochannel. The chemical compound forms a reversible bond with at least one analyte binding compound introduced into the nanochannel. Methods for making and using the reusable coating are also disclosed.
    Type: Grant
    Filed: October 15, 2013
    Date of Patent: July 17, 2018
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Natalie Gunn, Jose M. Lobez Comeras, Priscilla Rogers, John Wagner
  • Patent number: 9943843
    Abstract: The subject of the present invention is a microfluidic circuit in which are defined microchannels able to contain fluids and including at least one device for forming drops of a solution, guiding the drops to a storage zone in which one of the drops can be brought into contact with a drop of another solution, the walls of the microchannel portion forming the first drop-formation device diverging so as to cause drops of the first solution to detach under the effect of the surface tension of the first solution; the first guide include wall portions of the microchannels that diverge so as to cause the drops to move along under the effect of the surface tension of the first solution.
    Type: Grant
    Filed: October 8, 2013
    Date of Patent: April 17, 2018
    Assignees: ECOLE POLYTECHNIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Charles Baroud, Paul Abbyad, Etienne Fradet, Rémi Dangla
  • Patent number: 9890424
    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.
    Type: Grant
    Filed: June 17, 2015
    Date of Patent: February 13, 2018
    Assignee: Life Technologies Corporation
    Inventors: Jonathan Schultz, Todd Roswech, Jon A. Hoshizaki, Albert L. Carrillo, James A. Ball
  • Patent number: 9886041
    Abstract: A method for configuring a fluid control unit including the provision of a processing device with information on the fluid control unit, the moving device and fluid control valves, the determination of possible basic states of the moving device for the fluid control valves and/or of possible movement sequences, and the selection of a basic state and/or of a movement sequence by a user, the transmission, of a respective configuration file to the fluid control unit including open- and/or closed-loop control parameters for the selection of the fluid control valves, the use of the open- and/or closed-loop control parameters from the configuration file in the fluid control unit for selecting the fluid control valves for setting the selected basic state and/or for carrying out the selected movement sequence.
    Type: Grant
    Filed: January 18, 2012
    Date of Patent: February 6, 2018
    Assignee: FESTO AG & CO. KG
    Inventors: Martin Maichl, Michael Berner
  • Patent number: 9878324
    Abstract: Fluidic devices and methods including those that provide storage and/or facilitate fluid handling of reagents are provided. Fluidic devices described herein may include channel segments positioned on two sides of an article, optionally connected by an intervening channel passing through the article. The channel segments may be used to store reagents in the device prior to first use by an end user. The stored reagents may include fluid plugs positioned in linear order so that during use, as fluids flow to a reaction site, they are delivered in a predetermined sequence. The specific geometries of the channel segments and the positions of the channel segments within the fluidic devices described herein may allow fluid reagents to be stored for extended periods of time without mixing, even during routine handling of the devices such as during shipping of the devices, and when the devices are subjected to physical shock or vibration.
    Type: Grant
    Filed: December 22, 2016
    Date of Patent: January 30, 2018
    Assignee: OPKO Diagnostics, LLC
    Inventors: Jason Taylor, Vincent Linder
  • Patent number: 9829426
    Abstract: Provided is a fluid analysis cartridge configured to analyze a fluid specimen, a fluid analysis sheet forming the same and a method of manufacturing the fluid analysis cartridge. The fluid analysis sheet includes at least one cut out part configured to form an intermediate layer of an inspection substrate, the cut out part including a flow passage structure including an inlet part in which a fluid is introduced and an inspection part in which the fluid is introduced to react with a reagent; and wherein the cut out part is further configured to include a first end adjacent to the inspection part and a second end adjacent to the inlet part, and wherein a minimum distance between the first end and the flow passage structure is at least 1 mm.
    Type: Grant
    Filed: June 18, 2015
    Date of Patent: November 28, 2017
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Seung Jun Lee, Jung Ki Min, Seung Hoon Kim, Ji Young Park, Hyun-Suk Kang
  • Patent number: 9737912
    Abstract: There is provided a microparticle sorting method including a procedure of collecting a microparticle in a fluid that flows through a main channel in a branch channel that is in communication with the main channel by generating a negative pressure in the branch channel. In the procedure, a flow of a fluid is formed that flows toward a side of the main channel from a side of the branch channel at a communication opening between the main channel and the branch channel.
    Type: Grant
    Filed: August 9, 2013
    Date of Patent: August 22, 2017
    Assignee: Sony Corporation
    Inventor: Tatsumi Ito
  • Patent number: 9649632
    Abstract: The present disclosure sets forth incorporating microfluidic chips interfaces for use with digital microfluidic processes. Methods and devices according to the present disclosure utilize compact, integrated platforms that interface with a chip upstream and downstream of the reaction, as well as between intermediate reaction steps if needed. In some embodiments these interfaces are automated, including automation of a multiple reagent process. Various reagent delivery systems and methods are also disclosed.
    Type: Grant
    Filed: June 10, 2013
    Date of Patent: May 16, 2017
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: R. Michael Van Dam, Gaurav Shah, Pei-Yuin Keng
  • Patent number: 9651489
    Abstract: An assay device includes: a liquid sample receiving zone; a capture zone in fluid communication with the sample receiving zone having capture elements bound thereto, the capture zone having a substrate and projections which extend substantially vertically from the substrate, the projections having a rhombus-shaped cross-section and the projections arranged on the substrate with the corners of the projections facing upstream in a direction toward the sample receiving zone. The projections have a height, cross-section and a distance between one another that defines a capillary space between the projections capable of generating capillary flow parallel to the substrate surface; and a sink in fluid communication with the capture zone having a capacity to receive liquid sample flowing from the capture zone. The sample receiving zone, the capture zone and the sink define a fluid flow path. Preferably, the rhombus-shaped cross-section is a square or a diamond.
    Type: Grant
    Filed: April 4, 2012
    Date of Patent: May 16, 2017
    Assignee: Ortho-Clinical Diagnostics, Inc.
    Inventors: Zhong Ding, David Bergman