Abstract: A biological and chemical sample processing device that b. comprises a high pressure-resistant, shallow and wide area microfluidic chamber having at least one wall formed by a detachable slide containing samples such as immobilized entities, biological samples or molecules, c. comprises an arrangement of microfluidic access holes for injecting to and collecting fluid form said chamber, d. is interfaced with inlet ports and microfluidic channels which are formed external to the chamber, e. is configured so that the slide may be brought into contact with the device to form the said chamber, f. is adapted to deliver and to transport fluidic substances and reagents inside said chamber in a fast manner, preferably within less than 15 seconds, and in a regular or uniform way owing to said arrangement of microfluidic access holes.

Abstract: A configurable tissue processing system is disclosed that includes a plurality of configurable tissue processing stations, each station configured to receive tissue samples and selectively configurable to heat, agitate and apply either a positive pressure or a vacuum pressure to the received tissue samples; a transport mechanism that is configured to transport the tissue samples between the plurality of configurable tissue processing stations; and a control unit coupled to the plurality of configurable tissue processing stations and to the transport mechanism, the control unit controlling the transport mechanism to selectively position the tissue samples in the plurality of configurable tissue processing stations, and the control unit configuring each of the plurality of configurable tissue processing stations to heat, agitate and apply either a positive pressure or a vacuum pressure to the received samples. Also disclosed is a method of configuring the plurality of tissue processing stations.

Abstract: A tissue handling device is disclosed. A tissue collecting device is disclosed. A cassette for handling biological tissues is disclosed. A tissue dyeing device is disclosed. A method for handling biological tissues is disclosed. A method for dyeing biological tissues is disclosed.

Abstract: The invention relates to a system for collecting a biological sample comprising a container having at least one open end, a closure fitting on or in said at least one open end, a holding element connected to said closure and a solid matrix on which said biological sample is deposited, and optionally at least one processing agent, wherein said solid matrix is at least partially transferable into a liquid or dissolved state by changing at least one physico-chemical property of the environment of said matrix without disintegration of the biomolecules comprised in said biological sample deposited on said matrix.

Abstract: A cartridge configured for use in a blood analyzer is provided. The cartridge may include a substantially rigid frame, a flow path within the rigid frame, at least one opening in the substantially rigid frame configured to align and stabilize a capillary tube, and a seal within the flow path. The seal may be configured to temporarily obstruct flow through at least a portion of the flow path. The seal may also be configured to open in response to a force exerted via a capillary tube inserted into the at least one opening.

Abstract: An apparatus (10) for handling specimen slides comprises a first module receiving region (18) in which a coverslipping module (100, 200), for coverslipping thin sections arranged on the specimen slides with a coverslipping medium and a coverslipping element, is receivable. A first electrical interface is provided in the first module receiving region (18) for attachment of a coverslipping module (100, 200), this interface being embodied so that by way of it, both a glass coverslipping module (100) for coverslipping with glass wafers and a tape coverslipping module (200) for coverslipping with tapes is attachable. The first module receiving region (18) is furthermore embodied in such a way that a tape coverslipping module (200) or a glass coverslipping module (100) is selectably receivable in it.

Abstract: An apparatus and method are described that achieve independent and simultaneous processing of a plurality of substrate-supported biological samples. In one embodiment, substrate holders arranged in a minor arc are independently moveable between a processing position and an access position, and reagents are delivered to substrates held in the substrate holders through a nozzle plate that moves along the arc of substrate holders. The disclosed apparatus and method are particularly suited for implementation of lean processing of biological samples.

Abstract: A protective sheath for prolonged storage of pathology paraffin blocks. The sheath protects the paraffin block against gouging, scratching, denting, rodents, and insects. A pathology slide slides into a slot in the protective sheath and is held into place by retainers running on the interior face of the protective sheath. A paraffin block cassette accepts a paraffin block and slides into a sample block sleeve holder disposed on one edge of the sheath.

Abstract: This invention provides compositions and methods useful for the processing of a tissue sample and the preparation of cells, cell aggregates and/or tissue fragments. The invention provides two-stage filer devices and two-membrane devices. Cell aggregates and/or tissue fragments prepared using such devices and according to methods of the present invention can be used in a variety of assay systems, including, but not limited to, drug validation assays, drug screening assays, proliferation assays, metabolic assays, metastasis assays, angiogenesis assays, binding assays, biochemical assays, cellular assays, genetic assays, and the like.

Abstract: A method for fixation of organic tissue samples includes immersing a tissue sample in a solution of Formalin at a temperature of between 2° C. and 10° C. for a first time period (A), and immersing the tissue sample in a cold fixation dehydrating agent for a second time period (B) following the first time period. An automated tissue fixation system for fixating organic tissue samples in Formalin is also described.

Abstract: A container for storing a biological sample for molecular diagnostic testing and/or histological testing is provided. The container includes a first chamber for receiving a sample holder therein, a second chamber, and a closure for enclosing the container. A breakable membrane, such as a piercable foil, extends within the container and separates the two chambers. When the breakable membrane is broken, fluid can pass between the first and second chambers. The membrane may be broken through an activator on the closure, such as a depressible member or a rotatable carrier, causing the sample holder to break through the membrane.

Abstract: The present invention concerns an apparatus for automatic processing at least one biological sample accommodated on a carrier member, such as slide by applying a predetermined amount of reagents in a predetermined sequence according to a processing protocol, said apparatus comprising; a housing frame; at least one processing section for accommodating at least one slide, the at least one processing section is provided within the housing; a hood cover protecting the at least one processing section in said housing; wherein the hood cover completely encloses the processing section defining an interior space; and wherein the apparatus further comprises climate control device provided to control the environment within the interior space.

Abstract: The invention relates to a processor for working on histological samples. The processor has an inlet for the insertion of a fixing container, particularly a closed fixing container, with the samples located in the latter.

Abstract: An apparatus for holding a tissue sample including a retaining member having a first tissue engaging surface and at least one biasing element, the first tissue engaging surface being moveably attached to the retaining member by said biasing element; and a base having a second tissue engaging surface and configured to engage the retaining member to form an interior area with the first and second tissue engaging surfaces facing each other, wherein the at least one biasing element urges the first tissue engaging surface toward the second tissue engaging surface to retain the tissue sample therebetween in the interior area.

Abstract: The invention relates to an apparatus for treatment of histological samples. The apparatus includes at least a working station, in which the sample gets into contact with a liquid reagent. Further, the apparatus includes a purification device, which blows off at least a part of the reagent adhering to the sample and/or the sample holding arrangement by means of a gas flow, or which sucks off at least a part of the reagent adhering to the sample and/or a sample holding arrangement.

Abstract: An apparatus for holding a tissue sample having a retaining member with a first tissue engaging surface and at least one biasing element. The first tissue engaging surface is moveably attached to the retaining member. The apparatus also has a base comprising a second tissue engaging surface which is configured to engage the retaining member to form an interior area with the first and second tissue engaging surfaces facing each other. The apparatus also has a retracting member connected to the retaining member which is configured to retract the first tissue engaging surface and compress the biasing element to form a gap between the tissue sample and one of the first tissue engaging surface and the second tissue engaging surface.

Abstract: In accordance with one aspect of the invention, a mold is provided for receiving a tissue specimen and molten embedding material that improves the ease of withdrawing the embedded tissue specimen. The mold has a barrier coating intimately bonded to one or more surfaces of the mold that repels the attraction of the embedding material to the one or more mold surfaces and produces a positive meniscus of the embedding material in the mold. The barrier coating permits the mold to be re-used without needing to manually re-apply a release agent to the mold before using the mold. Further, the barrier coating permits the use of more aggressive draft angles than conventional molds by reducing the frictional engagement between the embedding material and the mold cavity surfaces.

Abstract: The invention relates to a valve structure for a microfluidic channel, where the valve serves the opening and closing of a channel (8) on a second level raised through channel pillars from the plane of the microfluidic channel network at the first level of a base plate (1), and where the channel wall is formed of a resilient material. The valve structure consists of a base plate (1) part, of protruding supporting walls (3, 3?) belonging to the base plate (1) and of a resilient part (7) between the supporting walls (3, 3?), within which the channel (8) is formed, and it consists furthermore of pressing appliances (9, 9?) situated on the two sides of the channel, as well as of displacement appliance (10) ensuring the movement of the pressing appliances in the direction of the mid-line of the channel (8).

Abstract: A system is capable of detecting substrates and can differentiate between zero, one, or multiple transparent or semi-transparent substrates in a stack. The system can include an optical sensor, an optically anti-reflective element, and a detector. The optical sensor outputs light towards the optically anti-reflective element. The light detector is positioned to detect light from the light source that is reflected by substrates, if any, positioned within a detection zone between the optically anti-reflective element and the detector.

Abstract: A cell isolation instrument includes: a first container which has an opening in an upper portion; a filtration member which defines at least a part of a lower portion of the first container; an isolation member which is movably housed in the first container to collide with a tissue, thereby isolating cells; and a second container which houses the first container in a manner that the first container can be taken out.

Abstract: Improved methods and systems for processing of solid tissue are described. The method may be performed manually or automatically. The system may have modules such as (i) a grossing module where a fresh tissue is sliced to prepare a tissue specimen, (ii) a hardening module that hardens the tissue specimen, (iii) an impregnating module that impregnates the tissue specimen that was hardened, and (iv) an embedding module that embeds a tissue specimen that was hardened and impregnated. Fresh (i.e., not fixed or frozen) tissue, which was excised to diagnose disease or to assess surgical treatment, is grossed to about 0.6 mm Preferably, the hardening of fresh tissue is initiated, but not completed, during grossing by contact with a chemical admixture. Preferably, dry ice, a thermoelectric device, or a gas condenser cools a metal mold containing the embedded specimen.

Abstract: A diagnosis assisting system comprising: an analyzing section for analyzing a sample collected from a subject; a determining section for determining whether there is a possibility that a subject has a predetermined disease based on an analysis result obtained by the analyzing section; a notification section for notifying the possibility when the determining section has determined that there is the possibility; and a diagnosis assisting information display section for displaying a diagnosis assisting information screen which displays diagnosis assisting information related to the predetermined disease, is disclosed. A diagnosis assisting information providing device and a computer program product are also disclosed.

Abstract: A container for storing a biological sample for molecular diagnostic testing and/or histological testing is provided. The container includes a first chamber for receiving a sample holder therein, a second chamber, and a closure for enclosing the container. A breakable membrane, such as a piercable foil, extends within the container and separates the two chambers. When the breakable membrane is broken, fluid can pass between the first and second chambers. The membrane may be broken through an activator on the closure, such as a depressible member or a rotatable carrier, causing the sample holder to break through the membrane.

Abstract: The invention relates to a biological microglia comprising at least two pores having a size adopted to allow cells, cell aggregates, tissue or other biological material to pass through said pores, and one or several slicing beams separating said pores from each other, wherein biological material is split/sliced/cleaved into at least two parts when passing said microgrid, a slicing device, an apparatus comprising said slicing device as well as the use of said microgrid, slicing device and apparatus.

Abstract: Methods and apparatus for selectively accessing a portion of a sterile cryopreserved sample are disclosed. The apparatus may include a container configured to receive the cryopreserved sample and having a first portion and a second portion, a heat sink chamber surrounding the first portion of the container, and a heat source adjacent to the second portion of the container. The chamber may be configured to maintain a non-accessed portion of the sample in a cryopreserved state. The heat source may be configured to separating an accessed portion of the sample from the non-accessed portion of the sample while maintaining the viability of the accessed portion while the non-accessed portion is maintained in the cryopreserved state.

Abstract: Tissue orientation devices include a perforated tissue support with at least one perforated channel for receiving a tissue sample, and a plurality of tabs configured to extend along and into the channel to retain the tissue sample during processing and embedding. Tissue orientation devices include elongated legs coupled together for holding one or more biopsy tissue samples therebetween. Associated methods include using the cassettes and orientation devices to hold and orient tissue samples for processing, embedding and microtome sectioning.

Abstract: A method and apparatus for embedding cells that utilizes a flow-through embedding technique. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway. The apparatus produces an embedded cell block having concentrated cells near the plane of the block to be sectioned in a quick and efficient manner.

Abstract: The invention relates to a method for execution upon processing of at least one histological sample that is arranged, in particular after an infiltration process, in a closed cassette (2). The method is characterized in that prior to opening of the cassette (2), the sample is detached from the cover of the cassette (2) and/or isolated.

Abstract: A handheld tool is disclosed which may be used to transfer a plurality of plant tissue explants from a first container to a second container. The handheld tool may include a disposable tip member which couples the plurality of plant tissue explants through use of negative pressure. An automated system which transfers a plurality of plant tissue explants from a first container to a second container is also disclosed. The automated system may include a first presentment system which moves the first container to a region, a second presentment system which moves the second container to the region, and a robot system that transfers the plurality of plant tissue explants from the first container to the second container.

Abstract: The disclosure relates to a method for fixing a histological sample, in which a target fixing time for the sample is defined and the sample is exposed to the action of a fixative, the exposure start time being stored in a memory associated with the sample. The sample is no longer exposed to the fixative when an action time of the fixative on the sample is equal to or greater than the target fixing time.

Abstract: The disclosure relates to a processor for processing histological samples. The processor comprises an inlet for insertion of a closed fixing container having the samples present therein.

Abstract: An apparatus for automatic execution of different treatment operations in connection with staining of tissue specimens on microscope slides, wherein the apparatus (1) comprises an assembly of vessels (4) for receiving different liquids for staining of the tissue specimens, a loading station (2) for microscope slides (28), a conveyor (5) for transfer of carriers with microscope slides from vessel to vessel in accordance with a treatment program, an unloading station (8) for treated microscope slides, and a control unit (18) for controlling the treatment operations in accordance with a data program. The apparatus comprises different levels (I, II) having units for execution of the relevant treatment operations.

Abstract: Automated sample processing systems may include onboard efficient high-speed mixing of at least two components with an automatic vertical force fluidic turbulent component mixer of which a mixed component may be aspirated and high-speed dispensed in a mixing vial. Other aspects may include single sweep applying a multi-treatment cleaning cycle to at least one slide. A multi-treatment cleaning cycle may include a washing treatment and a drying treatment. In yet other aspects the present invention may include an automated recovery sample processing system with the capability of detecting at least one immediate condition of a fortuitously terminated automatic sample processing run and perhaps even an automatic terminated sample processing run reconstruction calculator.

Abstract: The present invention is an apparatus and fluid delivery system for automated assays. The apparatus, for example, may be used for western blotting that uses flow lines for multiple reagents. In one embodiment, the present invention comprises a tray base with one or more fixed or disposable trays for testing samples, multiple flow lines, and multiple valves and the system is used for automatic controlled delivery of different fluids from supply vessels to different trays and discharges them to designated reservoirs or sewage system with minimum of operator actions. The system may include air line to assist supply and discharge the fluids and also coupled with measuring vessels with adjustable volume and valves adapted to interface with a valve operating unit with drive means.

Abstract: An apparatus (10) for handling specimen slides (32, 78, 80) has at least one coverslipping module (14) for coverslipping thin sections arranged on specimen slides (32, 78, 80). The apparatus (10) further encompasses a drying unit (16, 70, 90) for extracting solvent from the mounting medium applied onto the specimen slides (32, 78, 80). The drying unit (16, 70, 90) has an air delivery unit (40) for delivering an air flow to the specimen slides (32, 78, 80).

Abstract: An apparatus including at least one of a stainer module and a coverslipper module; an imaging module; a storage module; an automated transport module for transporting at least one slide between at least one of the stainer module and the coverslipper module, the imaging module and the storage module; and a controller. A method including processing at least one slide; determining whether an imaging module is available for imaging of a biological specimen on the at least one slide; transporting the at least one slide to the imaging module using an automated transport module; and transporting the at least one slide to a storage module using the automated transport module when it is determined that the imaging module is not available. A system including a processing module for processing at least one slide including a biological specimen thereon. A machine readable medium.

Abstract: A specimen holding and positioning apparatus operable to substantially non-movably maintain a specimen (e.g., an excised tissue specimen) in a fixed or stable orientation with respect to the apparatus during imaging operations (e.g., x-ray imaging), transport (e.g., from a surgery room to a pathologist's laboratory), and the like for use in facilitating accurate detection and diagnosis of cancers and/or other abnormalities of the specimen.

Abstract: A laser microdissection device includes a microscope stage for carrying a specimen to be cut, a laser light source for generating a laser beam and a microscope objective for focusing the laser beam onto the specimen. An optical laser scanning device is configured to deflect the laser beam so as to move the laser beam focus in an X-Y direction perpendicular to a main axis of the microscope objective. The laser microdissection device also includes at least one of a Z displacement device configured to move the microscope stage in a Z direction, or an optical focus shifting device configured to move the focus of the laser beam in the Z direction. The Z displacement device and/or optical focus shifting device are controllable, together with the optical laser scanning device, so as to move the laser beam focus in the specimen along a three-dimensional cut line in an X-Y-Z direction.

Abstract: An automated machine for handling and embedding tissue samples contained on microtome sectionable supports. The machine includes an input member configured to hold a plurality of the microtome sectionable supports prior to a tissue embedding operation. An output member is configured to hold a plurality of the microtome sectionable supports after the tissue embedding operation. A cooling unit is configured to hold at least one of the microtome sectionable supports during the tissue embedding operation. A motorized carrier assembly is mounted for movement and configured to hold at least one of the microtome sectionable supports. The carrier assembly moves the support from the input member to the cooling unit and, finally, to the output member. A dispensing device dispenses an embedding material onto the microtome sectionable support and at least one tissue sample carried by the microtome sectionable support during the embedding operation.

Abstract: Method and apparatus for processing paraffin embedded samples, e.g., to disassociate paraffin from tissue components and/or other biomolecules from the paraffin. The sample may be exposed to focused acoustic energy while held in a vessel containing a non-solvent, aqueous solution. Disassociated paraffin may be emulsified into the liquid or otherwise separated from the sample.

Abstract: A protective sheath for prolonged storage of pathology paraffin blocks. The sheath protects the paraffin block against gouging, scratching, denting, rodents, and insects. A pathology slide slides into a slot in the protective sheath and is held into place by retainers running on the interior face of the protective sheath. A paraffin block cassette accepts a paraffin block and slides into a sample block sleeve holder disposed on one edge of the sheath.

Abstract: An apparatus for sensing specimen slides (38) has a specimen slide holder (42) holding specimen slides (38), each specimen slide (38) comprising an identification code. The specimen slides (38) are arranged inside the specimen slide holder (42) on a lifting element (50) in a lifted position. The apparatus includes a reading apparatus (40) for reading out the identification code, and a positioning apparatus that moves the specimen slide holder (42) with the specimen slides (38) relative to the lifting element (50) so that the specimen slides (38) drop successively from the lifted position on the lifting element (50). As one of the specimen slides (38) drops, the identification code of the specimen slide (38) or of the subsequent specimen slide (38) becomes readable for the reading apparatus (40). A triggering apparatus (67, 68) triggers the reading apparatus (40) to read the identification code.

Abstract: Improved specimen containers, methods and materials for use in connection with excised tissues are described.

Abstract: The invention relates to a method for preparing specimens from tissue samples for cytology microscopic examination, and includes inventive compositions and a device used in said method. The invention also includes a diagnostic kit comprising the inventive compositions and device.

Abstract: A histology tissue embedding apparatus having an outlet for embedding medium and a generally horizontal working area beneath the outlet for supporting a cassette. The working area includes a generally horizontal trimming surface having corrugations and being substantially flush with the remainder of the working area.

Abstract: The invention relates, in accordance with one aspect, to a portable device for transporting at least one histological sample or at least one cassette containing a histological sample. The device is attachable to a processing station for processing 5 histological samples. In the attached state, energy for cooling the histological sample is transferred from the processing station to the device or vice versa. In accordance with another aspect of the invention, a processing station for processing a histological sample is provided, which station is embodied for releasable attachment of a portable device. The processing station is further embodied to transfer energy 10 for cooling to, or receive it from, an attached device.

Abstract: An automated processing machine, in particular an automated coverslipper or an automated stainer used for processing samples placed on slides and having an output device for outputting slides that have been processed by the automated processing machine. The output device includes an extensible and retractable drawer unit. The drawer unit includes at least one receiving channel for receiving a plurality of holders for slides. Holders received in any position in the receiving channel can be removed therefrom when the output device is in the open position. The automated processing machine is designed such that holders intended for output are inserted into the at least one receiving channel.

Abstract: Method and apparatus for holding and/or treating a sample material. A sample material may be positioned in a vessel between top and bottom flexible films where the flexible films are connected together by a substantially rigid support structure that surrounds the sample material. A crushing force may be applied to the sample material via the top and bottom flexible films, e.g., to pulverize the sample at cryogenic temperatures. A sample holder may have two vessels, one arranged for applying a crushing force to a first sample and another for holding a sample for other processing, such as a histology analysis.

Abstract: A process for the production of paraffin sections of biological tissue, especially for molecular pathology studies is disclosed. In the process, the tissue sample is simultaneously fixed, dehydrated and cleared in a first step, subsequently dehydrated and cleared in a second step and infiltrated with an inert specimen matrix in a third step. The specimen can then be further embedded in a casting supporting matrix according to the standard procedures followed by any local pathology or research laboratory. A kit and a processing station for automating paraffin embedding of a tissue sample suitable for histopathological and molecular analysis is also described. A bio-indicator system is described for measuring the degree of crosslinking. A tissue sample holding means or a vial which includes a tissue sample holding means provided with a data logging device capable of registering and transmitting data regarding the sample and conditions where the sample was processed is also disclosed.

Abstract: A method and a system for use of treatment liquids in an apparatus for staining of tissue specimens on microscope slides, wherein the apparatus comprises a plurality of vessels (4) for receiving different liquids for treatment of the tissue specimens, a plurality of tanks (11) for treatment liquids, connecting lines (15) between the tanks and the vessels, a plurality of pumps (13) connected on the connecting lines, and a control unit for control of the apparatus and the liquid treatment by means of a data program, wherein the control unit is arranged for selective control of the relevant pumps (13), so that vessels (4) to be used in a staining process, are filled with liquid (14) at the start of the staining process, and so that the liquid-filled vessels (4) are emptied automatically when they are no longer to be used in the staining process.