Abstract: A biochemical analysis system capable of sample preparation and processing can include at least one inlet channel having a non-fouling, slippery surface to autonomously transport a fluid sample to a chamber by a geometry of the at least one inlet channel. The at least one inlet channel can include a first end, which is open and exposed, and a second end connected to the chamber for mixing and reaction of the fluid sample, and the at least one inlet channel can include a converging or diverging angle.

Abstract: Methods for isolating viable cancer cells from a sample that comprises a mixture of cancerous cells and normal (non-cancerous) cells are provided. In the methods, a fluid preparation comprising a mixture of cancerous and normal cells is repeatedly exposed to fluid shear stresses, whereby the repeated exposure to the fluid shear stresses preferentially imparts fluid shear stress-resistance to the cancerous cells.

Abstract: A medical analyzer and coagulation profiler performs various interrogations on specimens. A motor with reduction gearing moves and a video camera observes the samples, the cartridges or parts thereof. Changes in images are compared and recorded with a central processor that controls a display. Power supply, temperature controller, motor and gearing are mounted in a box which attaches to a smartphone. The smartphone provides the video camera, illumination and central processor that control the movement, temperature and display. The device makes testing simpler for small hospitals, clinics, ambulances, remote locations and individuals and controls a number of parallel or serial devices operating simultaneously or sequentially. A cartridge insertion actuates a circular motion to generate a blood profile based on changes. Change is analyzed with a video camera and processor such as in a smartphone and is plotted to show an amplitude and time. A smartphone provides a specific movement pattern.

Abstract: A dual light source lens-free dielectrophoresis (DEP) flow cytometer for massively parallel single cell analysis. Each cells dielectric is inferred from measuring their altitude and subsequently velocity change due to DEP actuation in a microfluidic channel. Dual LED sources facilitate velocity measurement by producing two shadows for each cell passing through the channel. These shadows are detected using a linear optical array detector. Massively parallel analysis is possible as each pixel of the detector can independently analyze the passing cells. The DEP cytometer is composed of simple modular components and has the potential to be scaled to achieve a significantly high throughput label-free single-cell analyzer.

Abstract: A funnel-shaped extension attachment is provided for a blood collection device. The attachment provides a larger area for collecting a fingertip blood sample from a patient than the collection device itself would otherwise provide. The funnel is preferably easily removable from the collection device after the blood sample is taken. Optional trenches running down one or more corners of the attachment may be used to encourage pulling blood drops from the finger tip through capillary action. A tube may also provide another location to encourage pulling blood drops from the finger tip through capillary action. The funnel attachment may be mated with the collection device at a point of manufacture, or separately shipped and installed over the collection device at a point of use.

Abstract: Disclosed herein is an apparatus comprising: a probe carrier, an optical system and a sensor; wherein the probe carrier comprises a substrate, a first layer and a second layer; wherein the substrate comprises a first surface, a second surface, one or more locations on the first surface configured to be deposit sites for one or more probes; wherein the second surface is at an opposite side of the substrate from the first surface; wherein the first layer is on the first surface of the substrate or is embedded in the substrate under the first surface; wherein the second layer is on the second surface of the substrate or is embedded in the substrate under the second surface; the first and second layers are configured to reduce crosstalk between probes at different locations.

Abstract: The present invention relates to a method for the measurement of at least one sample by the interaction with the surface in the field of at least one sensor surface, such as surface plasmon resonance measurement, comprising the steps of: i) sampling the sample and a buffer; ii) transporting the sample and the buffer to at least one flow cell which is in liquid contact with the sensor surface of at least one sensor for measuring a parameter of a sample by interaction of the sample at the sensor surface in the field of the sensor surface; iii) transporting the sample into the flow cell into contact with the sensor surface; iv) handling a separation fluidum by inserting and/or removing the separation fluidum by between the sample and the buffer upstream and/or downstream of the sensor surface; v) measuring the interaction of the sample at the sensor surface; and vi) dispensing the sample from the flow cell, and to a measuring system for such method.

Abstract: A sample testing cartridge is usable to perform a variety of tests on a viscoelastic sample, such hemostasis testing on a whole blood or blood component sample. The cartridge includes a sample processing portion that is in fluid communication with a sample retention structure. A suspension, such as a beam, arm, cantilever or similar structure supports or suspends the sample retention portion relative to the sample processing portion in a unitary structure. In this manner, the sample retention portion may be placed into dynamic excitation responsive to excitation of the cartridge and correspondingly dynamic, resonant excitation of the sample contained within the sample retention portion, while the sample processing portion remains fixed. Observation of the excited sample yields data indicative of hemostasis. The data may correspond to hemostasis parameters such as time to initial clot formation, rate of clot formation, maximum clot strength and degree of clot lysis.

Abstract: Methods for determining the gelation period of a gel solution are provided. The methods provided include introducing an inert ball into each of a plurality of gel solution containers having a gel solution therein. In the method, the inert balls are then allowed to sink to the bottom of the containers, followed by a step of repeatedly inverting a first gel solution container at specified time intervals until the onset of gelation of the first gel solution is observed. Methods further include repeatedly inverting an additional gel solution container at specified intervals where each of the subsequent gel solution containers containing the inert balls and gel solutions are sequentially inverted in series until the inert ball is observed to remain fixed in place in the solution.

Abstract: An apparatus for examination of a free-flowing sample, comprising: a measuring chamber for receiving the sample, the measuring chamber having at least a portion which is transmissive to light of a spectral range; a casing configured to at least partially enclose the measuring chamber, the casing having at least of portion of its inner surface with a reflectivity in the spectral range; a light source for applying light of the spectral range to the measuring chamber; and a sensor for detecting light of the light source reflected by the sample.

Abstract: A thrombelastography device, a heating apparatus, a blood coagulation analysis system, and a rotational angle measurement method are disclosed. The thrombelastography device consists of a plurality of thrombelastography device splits (2) that are horizontally arranged in parallel. The thrombelastography device split (2) comprises a worktable (4), a rack (5), a test bar (6), a tester (8), and a processor (9). The thrombelastography device overcomes the defect in the prior art that the measurement result of a thrombelastography device is inaccurate. The amount of reflected light is used as a reference for thrombelastographic evaluation, and thus the result is more accurate.

Abstract: Disclosed herein is an apparatus comprising: a probe carrier comprising: a first substrate comprising a first plurality of through holes in the first substrate, a transparent window attached to the first substrate and across an opening of each of the first plurality of through holes, wherein the transparent window closes the opening; and probes attached to one or more locations on the transparent window, wherein interaction between the probes and an analyte generates a signal; a second substrate comprising a second plurality of through holes in the second substrate, wherein the second plurality of through holes are configured as a plurality of collimators; a sensor comprising a plurality of pixels configured to detect the signal.

Abstract: Among others, the present invention provides apparatus for detecting a disease, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electric, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, physical, or mechanical property of the biologic material.

Abstract: A biochemical analysis system capable of sample preparation and processing can include at least one inlet channel having a non-fouling, slippery surface to autonomously transport a fluid sample to a chamber by a geometry of the at least one inlet channel. The at least one inlet channel can include a first end, which is open and exposed, and a second end connected to the chamber for mixing and reaction of the fluid sample, and the at least one inlet channel can include a converging or diverging angle.

Abstract: The present application describes a new device and method of use thereof, which allows identifying certain antigens and antibodies present in the blood. The device of the present invention is a closed device consisting of two parts, wherein the upper part has a chamber surrounded by LEDs illuminating the analysis plate, which is supported by the rotating platform. In turn, the rotating platform is connected to a motor that will promote the rotation thereof for mixing reagents with blood. After a period of time, the camera will capture and send the resulting image to a computer program that will analyze the sample, using image processing techniques.

Abstract: A method for determining the concentration of a fluorescent and/or fluorescence-labeled analyte, and to a calibration method for preparing such determination, for use in the field of biological and environmental analysis in order to improve the accuracy of concentration determination, comprising the following steps: performing fluorescence measurements for calibration samples that have predetermined concentrations of a plurality of fluorescent and/or fluorescence-labeled reference analytes Rj that differ from each other by values m of a diffusion measure characterizing the diffusion of the reference analyte, in order to determine the values i of a concentration-dependent parameter I; establishing functions Fj(c)=i which describe the dependence of the parameter I on the concentration; determining the values of a slope parameter a for the respective reference analyte as a derivative of the respective function at c=0; determining the values mj of the diffusion measure for the reference analytes; establishing the

Abstract: A triple-chambered container includes: a main container body with a first chamber which has an opening at its distal end; a main barrel formed and movable longitudinally within the first chamber, the main barrel defining therein a second chamber for receiving fluids, the main barrel further having an apertured stopper at its distal end; a second barrel formed within the main barrel, the second barrel defining a third chamber for receiving fluids, the second barrel being movable longitudinally within and with respect to the main barrel, the second barrel having a distal end which is engageable and disengageable with the apertured stopper; a shaft adapted to fit within the second barrel, the shaft being movable longitudinally within and with respect to the second barrel, the shaft having a distal end which is engageable and disengageable with an aperture in the second barrel; a device for controlling engaging and disengaging of the distal end of the shaft with the aperture of the second barrel.

Abstract: A blood clot retraction assay device and methods of measuring whole-blood clot retractive forces during coagulation are described. When placed in proximity to a micro-beam, a blood droplet attaches thereto, applying a force to the micro-beam during clot formation. Using Piezoresistive micro-beams or other Piezoresistive components disposed upon such micro-beams, the device measures retractive force of a blood droplet based upon changes in resistance. The sensitivity of such measurements allows use of small quantities of blood for measurement, between 15 microliters and 300 microliters. The device further permits continuous measurement of coagulation, allowing measurement of distinct reaction, contraction development, and fibrinolysis phases for each blood sample.

Abstract: Provided is a method for evaluating fluid flow characteristics of a lens-free complementary metal-oxide semiconductor (CMOS) optical sensor package module with a flow channel. The method includes: measuring a propagation profile and a flow velocity in an initial state flow of a fluid in the flow channel; calculating a first statistical parameter relating to flow characteristics of the fluid from the measured propagation profile and flow velocity; and comparing the calculated first statistical parameter with a preset reference value and evaluating quality of the flow channel according to the comparison result.

Abstract: Some embodiments of a blood coagulation testing system include an analyzer console device and a single-use components configured to releasably install into the console device. In some embodiments, the blood coagulation testing system can operate as an automated thromboelastometry system that is particularly useful, for example, at a point-of-care site. The systems can be configured with features such as individual actuation systems for each measurement module, and firmware for initial and ongoing calibration and error detection.

Abstract: The present invention relates to a method of detecting a possible infection of malaria in a patient using a digital optical microscope.

Abstract: A particle container includes a measurement section in which a measurement region is formed and the measurement section measurably accommodates multiple particles in the measurement region. The measurement section is configured to change a width of the measurement region in the measuring direction of measuring the particles.

Abstract: The present disclosure relates to a sensor arrangement (1) for analyzing substances in a material, comprising a substrate (2), a first reception area (5) for receiving a first material, an electronic component sensor (3), a test area (4) that is in contact with the electronic component sensor (3), and a control device (10). The first reception area (5) and the test area (4) are arranged on the substrate (2). The control device (10) is configured for controlling the first reception area (5) and/or the test area (4) to move a portion of the first material from the first reception area (5) into the test area (4). Further, the present disclosure relates to a method for operating such a sensor arrangement (1).

Abstract: The disclosure relates to devices, systems and methods for image registration and annotation. The devices include computer software products for aligning whole slide digital images on a common grid and transferring annotations from one aligned image to another aligned image on the basis of matching tissue structure. The systems include computer-implemented systems such as work stations and networked computers for accomplishing the tissue-structure based image registration and cross-image annotation. The methods include processes for aligning digital images corresponding to adjacent tissue sections on a common grid based on tissue structure, and transferring annotations from one of the adjacent tissue images to another of the adjacent tissue images.

Abstract: This disclosure relates to an assembly for loading a sample liquid. The assembly comprises an entry port comprising an inlet and a gas vent, and a reservoir. The reservoir comprises a distal wall, a fin, and a continuous fluidic pathway. The fin comprises first and second surfaces and extends from the inlet towards the distal wall. The continuous fluidic pathway comprises a converging channel that is in fluidic communication with the inlet and is defined by the second surface of the fin, and a diverging channel that is defined by the first surface of the fin and is in fluidic communication with the gas vent. At a distal end of the fin, a width of the converging channel at most equals a width of the diverging channel, and the width of the converging channel at most equals a distance between the distal end of the fin and the distal wall.

Abstract: Provided herein is a method for determining the volume or hemoglobin content of an individual red blood cell in a sample containing a population of red blood cells. The method may be performed on a hematology analyzer. Also provided are a hematology analyzer for performing the method and a computer-readable medium containing programming for performing the method.

Abstract: The invention relates to the field of blood clotting diagnosis and relates to a kinetic method for determining the fibrinogen concentration in a human plasma sample according to the Clauss method.

Abstract: Some embodiments of a blood coagulation testing system include an analyzer console device and a single-use cartridge component configured to releasably install into the console device. In some embodiments, the blood coagulation testing system can operate as an automated thromboelastometry system that is particularly useful, for example, at a point-of-care site.

Abstract: The present invention addresses a problem in which, when examining blood properties in measurements of thrombus formation capacity and platelet function or the like, measurement data is changed as a result of sedimentation of blood in reservoirs during measurement. An erythrocyte membrane ion transport inhibitor, such as disodium 4,4?-dinitrostilbene-2,2?-disulfonate (DNDS) or 4,4?-diisothiocyanatostilbene-2,2?-disulfonic acid (DIDS), is used to inhibit erythrocyte sedimentation, and accurate measurement data is obtained.

Abstract: Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use.

Abstract: The present invention relates to ellagic acid formulations for performing coagulation assays that are highly stable for long term storage and reduce assay time. Particularly, aspects of the present invention are directed to a composition and method of preparing ellagic acid in a highly soluble format for use in a coagulation assay. For example, the ellagic acid may be solubilized in one or more of sodium hydroxide, methanol, a polyether compound, particularly polyethylene glycol, polyethylene oxide, or polyoxyethylene, and a cyclodextrin guest-host complex.

Abstract: A method for determining the bacterial charge in a liquid or semi-liquid product, including: preparing a quantity of liquid or semi-liquid product to be analyzed; keeping that quantity of liquid or semi-liquid product at a preset measuring temperature (Tm) and deriving values (?) representing an angle of electrical impedance of the liquid or semi-liquid product at predetermined time instants; deriving an indication of the presence of a bacterial charge in the liquid or semi-liquid product as a function of the time trend of the values (?) representing the angle of the electrical impedance.

Abstract: This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.

Abstract: The present disclosure relates to the generation of an activated platelet product in which one or more of the presence or absence of clots, the timing of clot formation (if present), and/or the mechanical strength of clots (if present) is controlled by the presence or concentration of calcium ions during the activation process. In certain embodiments, the calcium ion concentration is controlled in the presence of pulsed electric fields or a chemical activator (e.g., thrombin) as part of the activation process.

Abstract: A microfluidic chip having a micro channel for processing a sample is provided. The micro channel may focus the sample by using focusing fluid and a core stream forming geometry. The core stream forming geometry may include a lateral fluid focusing component and one or more vertical fluid focusing components. A microfluidic chip may include a plurality micro channels operating in parallel on a microfluidic chip.

Abstract: An apparatus for measuring blood clotting time includes a blood clot detection instrument and a cuvette for use with the blood clot detection instrument. The cuvette includes a blood sample receptor-inlet; a channel arrangement including at least one test channel for performing a blood clotting time measurement, a sampling channel having at least one surface portion that is hydrophilic, communicating with the blood sample receptor-inlet and the at least one test channel, and a waste channel having at least one surface portion that is hydrophilic, communicating with the sampling channel; and a vent opening communicating with the sampling channel. The sampling channel, the vent opening and the waste channel, coact to automatically draw a requisite volume of a blood sample deposited at the blood receptor-inlet, into the sampling channel.

Abstract: Silica particles having a thiol group on a surface thereof, and satisfying the following conditions (a) to (c): (a) a particle diameter is 20 to 1,000 nm; (b) a density of the thiol group on the surface of the silica particles is 0.002 to 0.2 piece/nm2; and (c) a ratio (B/A) in terms of an amount B (piece/particle) of the thiol group existing on the surface of the silica particles to an amount A of sulfur elements in the silica particles (the number of sulfur elements derived from thiol per silica particle) is 0.10 to 0.60.

Abstract: This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.

Abstract: A cell detection device and a cell detection method is provided with which it is possible to efficiently acquire images of cells to be measured. Cell detection device comprises flow cell through which a measurement specimen that contains particles is caused to flow, particle detector for detecting the particles in the measurement specimen supplied to flow cell, particle sorter for sorting particles that satisfy a detection condition and other particles on the basis of the result of detection performed by particle detector, specimen supply part for supplying, to flow cell, an image-capture specimen that includes detection-condition-satisfying particles that have been sorted by particle sorter, and particle-image-capture part for capturing images of the particles in the sorted image-capture specimen supplied to flow cell.

Abstract: The disclosure relates to devices, systems and methods for image registration and annotation. The devices include computer software products for aligning whole slide digital images on a common grid and transferring annotations from one aligned image to another aligned image on the basis of matching tissue structure. The systems include computer-implemented systems such as work stations and networked computers for accomplishing the tissue-structure based image registration and cross-image annotation. The methods include processes for aligning digital images corresponding to adjacent tissue sections on a common grid based on tissue structure, and transferring annotations from one of the adjacent tissue images to another of the adjacent tissue images.

Abstract: Provided are devices, systems and methods for evaluation of hemostasis. In some embodiments, an apparatus is disclosed comprising a housing; a plurality of test chambers located in the housing, the plurality of test chambers including chambers configured for measurements via a system that interrogates one or more viscoelastic properties of test samples in the test chambers, wherein the one or more viscoelastic properties is used to characterize dynamics of coagulation and/or fibrinolysis.

Abstract: Early warning of changing health and robustness is given by tracking of ease of morphological changes in blood cells obtained by comparing intensities in a first scattered light intensity angular distribution and intensities in a second scattered light intensity angular distribution, with the light being scattered by blood cells into very narrowly forward scattered light intensity angular range.

Abstract: A fluid-containing cartridge for a tissue processing apparatus includes a body defining a plurality of discrete fluid passageways, and a plurality of fluid-containing wells that are disposed on the body, wherein each fluid passageway of the body defines a fluid path between one of the fluid-containing wells and a fluid exit port that is configured to dispense fluid onto a laboratory slide.

Abstract: Systems, apparatuses and methods include evaluation the clotting time or strength of clotting in the presence of various clot-affecting reagents to obtain a profile of clot analysis for determination of bleeding complications. The various reagents may be included in a single cartridge for use in a blood clotting analysis device.

Abstract: A fluorescent particle measuring method comprising the steps of trapping a fluorescent particle included in a fluid that is to be evaluated; identifying, by a material analyzing device, a material of a fluorescent particle that has been trapped; measuring, by a fluorescent particle measuring device, a number of fluorescent particles included in the fluid; and identifying, as the material of a fluorescent particle that has been measured by the fluorescent particle measuring device, a material identified by the material analyzing device.

Abstract: A particulate measurement device includes: a nozzle which discharges liquid from an opening to form a flow of the liquid; a light emitter which emits light such that the light propagates in a region where the flow of the liquid is formed; a photodetector provided outside the region to receive the light from a partial region extending along a longitudinal direction of the region; and an air flow forming unit which forms a flow of gas along a direction in which the liquid flows, on an outer periphery of the region.

Abstract: A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.

Abstract: An integrated “lab-on-a-chip” microfluidic device performs nucleic acid sample preparation and diagnostic analysis from test samples containing cells and/or particles. The device analyzes DNA or RNA targets, or both, from a common test sample. Dried and/or liquid reagents necessary for nucleic acid sample preparation and analysis are contained on the device, such that the device only requires addition of test sample. Clay mineral and alkaline buffer reagents are employed for overcoming the problems of nucleic acid degradation and contamination during sample preparation. The device may include a composite filter to separate plasma or serum from other blood constituents when the test sample is a blood product. The microfluidic device utilizes a plurality of microfluidic channels, inlets, valves, membranes, pumps, and other elements arranged in various configurations to manipulate the flow of the liquid sample, in particular, in order to prepare nucleic acids and perform further diagnostic analysis.

Abstract: Disclosed is a sheath delivery system that uses a continuous flow of sheath fluid into a pressurized internal reservoir that substantially matches the outflow of sheath fluid through the nozzle of a flow cytometer. A substantially constant level of the sheath fluid is maintained. If the sheath fluid level falls below a desired level, or goes above a desired level, a dampened control system is used to reach the desired level. In addition, air pressure in the pressurized internal container is controlled so that an external sheath container can be removed and refilled with additional sheath fluid without stopping the sheath delivery system 100. Differences in pressure are detected by a droplet camera, which measures the droplet breakoff point to determine the pressure of the sheath fluid in the nozzle.

Abstract: A particle processing device includes a chamber and at least one capturing structure. The chamber is connected to a first port and a second port to provide a space between the first and second ports for flowing of a fluid having a particle. The capturing structure is provided in the chamber to form a fluidic channel, wherein the fluidic channel has a first opening and a second opening and a capturing region is formed between the first and second openings such that the capturing region has a changeable sectional shape for capturing the particle in the fluid flowing from the first port to the second port.