Abstract: Disclosed is a sheath delivery system that uses a continuous flow of sheath fluid into a pressurized internal reservoir that substantially matches the outflow of sheath fluid through the nozzle of a flow cytometer. A substantially constant level of the sheath fluid is maintained. If the sheath fluid level falls below a desired level, or goes above a desired level, a dampened control system is used to reach the desired level. In addition, air pressure in the pressurized internal container is controlled so that an external sheath container can be removed and refilled with additional sheath fluid without stopping the sheath delivery system 100. Differences in pressure are detected by a droplet camera, which measures the droplet breakoff point to determine the pressure of the sheath fluid in the nozzle.

Abstract: A particle processing device includes a chamber and at least one capturing structure. The chamber is connected to a first port and a second port to provide a space between the first and second ports for flowing of a fluid having a particle. The capturing structure is provided in the chamber to form a fluidic channel, wherein the fluidic channel has a first opening and a second opening and a capturing region is formed between the first and second openings such that the capturing region has a changeable sectional shape for capturing the particle in the fluid flowing from the first port to the second port.

Abstract: The present invention relates to an apparatus and a method for testing a function and a drug response of a platelet based on a microfluidic chip. The apparatus for test comprises a measuring device containing a blood sample and measuring aggregation and adhesion of a platelet generated by flow of the blood sample; and a fluid driving device which is connected to the measuring device and generates a oscillating shear flow of the blood sample. Therefore, activation of factors such as a platelet and von Willebrand factor (vWF) etc. can be activated uniformly and completely, and a repeatability of the platelet aggregation can be increased.

Abstract: A cell study device, comprising, a base layer, a planar conduit defining layer, including a conduit cut out of the layer; and a planar cover layer which defines a capillary flow channel in said conduit layer, said conduit layer and said cover layer acting as side walls for said capillary flow channel, wherein said layers are formed of materials that do not interfere with cell behavior over a period of at least 5 hours when loaded with aqueous solution.

Abstract: Provided are devices, systems and methods for evaluation of hemostasis. Also provided are sound focusing assemblies.

Abstract: Apparatus and methods are described for automatically performing set-up steps for flow cytometry operations. The invention provides for the spatial determination of a flow stream and the subsequent automatic alignment of analysis devices and/or collection vessels. The automatic determination of flow stream properties provides for the automatic configuration flow cytometer parameters.

Abstract: Disclosed herein is a microfluidic device comprising, at least one sample inlet for receiving biological cells in a biological fluid sample; at least one sheath flow inlet for receiving a sheath fluid; at least one curvilinear channel configured to provide the biological fluid sample substantially in an outer flow and the sheath fluid in substantially an inner separated flow; a plurality of cell traps at the periphery of the curvilinear channel, each trap configured to admit a single cell having a targeted size range from the outer flow.

Abstract: A sample analyzer comprising: a sample preparing section for preparing first and second measurement sample including reagent and sample; a first detector for detecting a predetermined component in the first measurement sample prepared by the sample preparing section; a second detector for detecting the predetermined component in the second measurement sample prepared by the sample preparing section; and a controller configured for performing operations, comprising: (a) controlling the first detector to detect the predetermined component in the first measurement sample prepared by the sample preparing section; (b) determining the reliability of the result detected by the first detector; (c) controlling the sample preparing section to prepare the second measurement sample from the same sample when the result has been determined to be unreliable; and (d) controlling the second detector to detect the predetermined component in the second measurement sample, is disclosed.

Abstract: An apparatus for determining blood clotting capacity comprises an actuator to cyclically move a member within a sample of blood received in a well in a tray and one of a deflection sensor and a position sensor to determine the position of the wetted member upon being acted upon by the actuator. The theoretical position of the wetted member, as determined using a known actuator force and wetted member physical data, is compared to the sensed deflection or position of the wetted member, and the resistance to movement of the wetted member caused by the blood is determined and correlated to a clotting capacity.

Abstract: An analyte measurement system is provided having sensors with embossed test chamber channels. In one embodiment, the sensors are elongate test strips for in vitro testing, each test strip having a substrate, at least one electrode, an embossed channel in the electrode, and lidding tape covering at least a portion of the embossed channel. Methods of manufacture are also disclosed for filling the sensor channels with reagent, and for trimming the ends of the sensors to eliminate the need for a calibration code during use of the sensors with a meter.

Abstract: A sample testing cartridge is usable to perform a variety of tests on a visco-elastic sample, such hemostasis testing on a whole blood or blood component sample. The cartridge includes a sample processing portion that is in fluid communication with a sample retention structure. A suspension, such as a beam, arm, cantilever or similar structure supports or suspends the sample retention portion relative to the sample processing portion in a unitary structure. In this manner, the sample retention portion may be placed into dynamic excitation responsive to excitation of the cartridge and correspondingly dynamic, resonant excitation of the sample contained within the sample retention portion, while the sample processing portion remains fixed. Observation of the excited sample yields data indicative of hemostasis. The data may correspond to hemostasis parameters such as time to initial clot formation, rate of clot formation, maximum clot strength and degree of clot lysis.

Abstract: Embodiments of the present invention encompass automated systems and methods for analyzing white blood cell parameters in an individual based on a biological sample obtained from blood of the individual. Exemplary techniques involve correlating aspects of direct current (DC) impedance, radiofrequency (RF) conductivity, and/or light measurement data obtained from the biological sample with an evaluation of white blood cell conditions in the individual.

Abstract: The present disclosure provides cytometric methods for the detection of rare target cells in a sample. In certain aspects, the methods and compositions may facilitate the detection of rare target cells, such as circulating tumor cells (CTCs), in a biological sample such as blood. Aspects of the methods include contacting the sample with first and second binding members that specifically bind to a marker of the rare target cell, and cytometrically assaying the sample for the presence of cells comprising bound first and second binding members to detect the rare target cell in the sample. Also provided are systems, compositions, and kits for practicing the subject methods.

Abstract: Embodiments of the present disclosure provide for devices, methods for separating particles, and the like. In general, embodiments of the present disclosure include non-uniform magnetic field-assisted processes and devices for the separation of particles (e.g., cells) within a magnetic fluid. Under non-uniform magnetic fields, particles such as cells can experience the generated magnetic field direction to produce a magnetic buoyancy force, analogous to buoyancy force, as magnitude of the force is proportional to the volume of cell. This force can be used to spatially separate cells of different sizes. In some embodiments, devices for separating particles are provided having a magnetic device configured to direct a non-uniform magnetic force onto the magnetic fluid and the particles; and a plurality of outlets, wherein the non-uniform magnetic force causes the types of particles to be separated and flow into different outlets.

Abstract: A method of characterizing the coagulation or sedimentation dynamics of a fluid such as whole blood, a blood fraction, or blood plasma is provided. The method includes illuminating a sample of the fluid with a beam of coherent light; acquiring a time series of images of a speckle pattern generated by interaction between the sample and the spatially coherent light beam; and processing the time series of images. The processing step includes calculating a function representative of the variation in the speckle pattern between two or more images of the series. The invention also provides a device for implementing such a method.

Abstract: Provided is technology for blood clotting reactions capable of analyzing a blood clotting reaction with a high degree of precision, by precisely detecting and removing noise, regardless of the location where the noise is generated in the light intensity data. This automated analyzer approximates, with an approximation curve, time series data for transmitted light intensity or scattered light intensity of light emitted onto a sample, and, in this process, removes abnormal data points that deviate from the approximation curve (see FIG. 2).

Abstract: The present invention relates to a method of enriching fetal nuclei from a sample. Enriched fetal nuclei can be used in a variety of procedures including, detection of a trait of interest such as a disease trait, or a genetic predisposition thereto, gender typing and parentage testing.

Abstract: A device for measuring permeability of a material sample, the device comprising: an input tank (1) configured to store fluid and to supply the fluid to a sample tank (2) positioned below the input tank (1) and configured to hold the material sample, such as to allow the fluid from the input tank (1) to permeate through the material sample; a receptacle (25) positioned under the sample tank (2) to collect drops of fluid permeated through the material sample; and a device (3) for measuring the mass of the drops collected in the receptacle (25) and coupled with a controlling and monitoring device (11) configured to store data on measured mass and measurement times.

Abstract: An analytical test cartridge is provided. The analytical test cartridge can be used for medical analyses of liquid samples removed from a patient, for example blood. The analytical test cartridge is configured to provide for titration experiments. An example of a titration experiment that can be performed with the arrangement, is titration of heparin with protamine. Methods of assembly and use are provided.

Abstract: A flow cell including inlet and outlet ports in fluid communication with each other through a flow channel that extends therebetween. The flow channel includes a diffuser region and a field region that is located downstream from the diffuser region. The field region of the flow channel directs fluid along reaction sites where desired reactions occur. The fluid flows through the diffuser region in a first flow direction and through the field region in a second flow direction. The first and second flow directions being substantially perpendicular.

Abstract: Device and process to approximate somatic cell count (SCC) of untreated mammalian milk by the two variable equation SCC=f (FSL, FAT) with a forward scattered light factor (FSL) being obtained by detecting light scattered by the milk into an angular range within, and less than, the angular range 0.0 to 0.5 degrees away from the central axis of incident light, with a proxy (FAT) for the fat content of the milk, which may be obtained by detecting light attenuation of the milk sample, and with the function (f) being obtained by calibration of the device using reference milk samples.

Abstract: A well plate (1) for holding samples of a bodily fluid during analysis thereof, typically in an analytical apparatus, includes a plate (2) having a plurality of first wells (3) extending downwardly therefrom for holding a sample during optical analysis of the sample, and a plurality of second wells (4) for holding samples during mechanical analysis of the samples. A plurality of holding wells (8) are provided for initially receiving and holding samples of the bodily fluid to be analysed so that samples of relatively accurate size can be pipetted from the holding wells (8) to the first and second wells (3,4).

Abstract: A MEMS-based system and a method are described for separating a target particle from the remainder of a fluid stream. The system makes use of a unique, microfabricated movable structure formed on a substrate, which moves in a rotary fashion about one or more fixed points, which are all located on one side of the axis of motion. The movable structure is actuated by a separate force-generating apparatus, which is entirely separate from the movable structure formed on its substrate. This allows the movable structure to be entirely submerged in the sample fluid.

Abstract: A method for inhibiting corrosion comprises the steps of providing a fluid; adding a corrosion inhibitor comprising at least one amphiphilic chemical to the fluid; and monitoring micelles presence in the fluid. A method for determining the amount of corrosion inhibitor that is sufficient to inhibit corrosion, a method for monitoring the activity of an amphiphilic chemical and a system for inhibiting corrosion in a conduit are also disclosed.

Abstract: In some embodiments, the invention provides methods for detecting fibrinolysis or hyperfibrinolysis in a blood sample from a patient. The method includes subjecting a first portion of a blood sample comprising reduced platelet function to viscoelastic analysis in the absence of an inhibitor of fibrinolysis to obtain a coagulation characteristic of the first portion at a time point; and subjecting a second portion of the blood sample comprising reduced platelet function to viscoelastic analysis in the presence of an inhibitor of fibrinolysis to obtain a coagulation characteristic of the second portion at the time point; wherein a difference between the coagulation characteristic of the first portion and the coagulation characteristic of the second portion indicates fibrinolysis or hyperfibrinolysis in the blood sample.

Abstract: In one embodiment, a device for measuring blood coagulation includes a substrate having a surface, an optical waveguide provided on the surface of the substrate, the waveguide having a tip, and means for spatially separating objects from the tip of the waveguide.

Abstract: An optical system for performing scattering and absorbance assays in clinical diagnostics comprises a light source for emitting collimated light parallel to an optical axis in an optical path, a sample holding unit comprising at least one sample holding position located in the optical path and an optical detector for measuring light transmitted through a sample located in a sample holding position. The optical system further comprises an adjustable light angle selector adjusted to prevent light transmitted through the sample and diverging from the optical axis with an angle greater than a certain value from reaching the detector when a scattering assay is performed, and wherein the light angle selector is adjusted to allow light transmitted through the sample and diverging from the optical axis with an angle smaller than a certain value to reach the detector when an absorbance assay is performed.

Abstract: Provided are devices, systems and methods for evaluation of hemostasis. Also provided are sound focusing assemblies.

Abstract: A sample analyzer capable of operating in a first measuring mode for measuring a sample and a second measuring mode for measuring a sample, comprising: a sample provider for providing a sample; a common reagent provider for providing a common reagent used in the first measuring mode and the second measuring mode; a special reagent provider for providing a special reagent used in the second measuring mode; a mode selector for selecting one of the first measuring mode and the second measuring mode; a measuring section for measuring the sample; and wherein in the first measuring mode, the sample provider and the common reagent provider operate so as to make a first mode sample comprising the sample and the common reagent, and the measuring section operates so as to measure the first mode sample, and in the second measuring mode, the sample provider, the common reagent provider and the special reagent provider operate so as to make a second mode sample comprising the sample, the common reagent and the special rea

Abstract: A system and method for capturing and analyzing cells comprising: a fluid delivery module; a reservoir configured to receive a biological sample including a target cell population and at least one fluid from the fluid delivery module; a manifold configured to receive and distribute the biological sample and at least one fluid from the reservoir into a cell capture device; a waste chamber configured to couple to the manifold; and a pump configured to couple to the waste chamber. In embodiments of the system configured to promote further purification of captured cells, the system can further comprise a magnet that enables further separation of captured cells from undesired sample materials. The system can additionally further comprise a heater configured to heat at least one fluid and/or biological sample, and a cell capture device configured to couple to the manifold, in order to facilitate capture of the target cell population.

Abstract: The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.

Abstract: There is provided a microparticle sorting device that automatically optimizes a fluid stream. There is provided a microparticle sorting device that includes a voltage supply unit that supplies a driving voltage to a vibratory element that applies vibration to an orifice that produces a fluid stream, a charge unit that imparts charge to at least some droplets ejected from the orifice, deflecting plates, arranged opposing each other with the fluid stream S therebetween, that vary a travel direction of the droplets, and a first image sensor that acquires an image of the droplets passing between the deflecting plates.

Abstract: A sample testing cartridge is usable to perform a variety of tests on a viscoelastic sample, such hemostasis testing on a whole blood or blood component sample. The cartridge includes a sample processing portion that is in fluid communication with a sample retention structure. A suspension, such as a beam, arm, cantilever or similar structure supports or suspends the sample retention portion relative to the sample processing portion in a unitary structure. In this manner, the sample retention portion may be placed into dynamic excitation responsive to excitation of the cartridge and correspondingly dynamic, resonant excitation of the sample contained within the sample retention portion, while the sample processing portion remains fixed. Observation of the excited sample yields data indicative of hemostasis. The data may correspond to hemostasis parameters such as time to initial clot formation, rate of clot formation, maximum clot strength and degree of clot lysis.

Abstract: An integrated fluidic device comprising includes an input chamber that provides an input of a sample fluid, and a first overspill chamber in fluid communication with the input chamber. A metering conduit is in fluid communication with the fluid input chamber and the first overspill chamber. The metering conduit meters a first metered volume of fluid from the sample fluid, and the first overspill chamber receives fluid in excess of the first metered volume of fluid. A second overspill chamber is in fluid communication with the metering conduit. The metering conduit meters a second metered volume of fluid from the first metered volume of fluid, and the second overspill chamber receives fluid from the first metered volume of fluid in excess of the second metered volume of fluid. The second overspill chamber has a fluid actuated closable valve for controlling the metering of the second metered volume of fluid.

Abstract: A multi-channel system and methods for sorting particles according to one or more characteristics of the particles. The system includes multiple flow cytometry units, each unit can have a nozzle for producing a fluid stream containing a desired population of particles in a mixture of particles. Each of the units may be operable to sort said desired population of particles by interrogating the fluid stream with a beam of electromagnetic radiation and classifying particles based on one or more characteristics of the particles. The system also includes a common fluid delivery system for delivering sheath fluid to each flow cytometer unit for producing respective fluid streams.

Abstract: A microfluidic sensor (10), such as an electrochemical blood test strip, with more accurate measurement comprises a plurality of channels (24) through which a fluid to be tested flows via a capillary action. One or more electrodes (30) are located under the channels (24). As the fluid flows over the electrodes (30) in the channels (24), the impedance between the electrodes (30) may be measured to determine fluid properties. In order to increase the accuracy of the measurements, the electrode deposition may be configured to be less than 10 ?m in thickness via a printing process with high process consistency, thereby reducing the disruption of the electrode deposition on the fluid flow.

Abstract: The present application provides apparatus and methods for determining the density of a fluid sample. In particular, it provides a sensor device which can be loaded with a fluid sample such as blood, and which further comprises at least one oscillating beam member or resonator. Exposure of the blood sample to clotting agents allows a clotting reaction to commence. The device allows the density of the sample fluid to be monitored with reference to the oscillation of the vibrating beam member, thus allowing the monitoring of the clotting of the fluid sample.

Abstract: The invention provides a device and a method for flow cytometric fractionation of particles contained in a fluid stream, wherein sections of the fluid stream, especially droplets if the fluid stream is a droplet stream, are irradiated with a laser. The laser disposed for the irradiation of the sections of the fluid stream can have a wavelength which is absorbed by the fluid and can have a sufficient radiation duration and radiation intensity to deflect sections of the fluid stream.

Abstract: One embodiment of the present invention is to provide a system for sorting cell particles in a liquid flow, which comprises a flow-defining block including a flow chamber receiving a sample conduit and a flow cell having a flow channel. The system also comprises a strobe block including a imaging device for taking an image of a jet flow ejected from the flow-defining block and a plurality of droplets in a given image area. The flow-defining block and the strobe block are detachably connected each other. Further the strobe block includes a nozzle plate having a nozzle channel between a receiving end and a nozzle. The flow-defining block includes a discharge end opposite to the receiving end and a channel enlarged portion of which cross section taken along a predetermined plane perpendicular to a flow direction has an area increasing towards the discharge end. The discharge end and the receiving end have substantially the same open distance in the predetermined plane perpendicular to the flow direction.

Abstract: A system and method are disclosed in which particles sorted by a flow cytometer may be deposited directly into a deposition layer formed on the surface of an optical disc, and information regarding measurements made of the particle, as well as the storage location of the particle, can be written to the recording layer of the optical disc.

Abstract: A specimen analyzing method and a specimen analyzing apparatus capable of measuring interference substances before analyzing a specimen. The method comprises a step for sucking the specimen stored in a specimen container (150) and sampling it in a first container (153), a step for optically measuring the specimen in the first container, a step for sampling the specimen in a second container (154) and preparing a specimen for measurement by mixing the specimen with a reagent in the second container, and a step for analyzing the specimen for measurement according to the results of the optical measurement of the specimen.

Abstract: A method may involve forming one or more photoresist layers over a sensor located on a structure, such that the sensor is covered by the one or more photoresist layers. The sensor is configured to detect an analyte. The method may involve forming a first polymer layer. Further, the method may involve positioning the structure on the first polymer layer. Still further, the method may involve forming a second polymer layer over the first polymer layer and the structure, such that the structure is fully enclosed by the first polymer layer, the second polymer layer, and the one or more photoresist layers. The method may also involve removing the one or more photoresist layers to form a channel through the second polymer layer, wherein the sensor is configured to receive the analyte via the channel.

Abstract: The invention provides a modified form of saturation assay, which is based on the measurement of a free or unbound labelled reagent fraction (such that there is an increase in signal in the presence of analyte) and employs trapping zones to concentrate said unbound or free labelled fraction to avoid loss of sensitivity. Preferably, the assay is a membrane assay.

Abstract: An integrated system for determining a hemostasis and oxygen transport parameter of a blood sample, such as blood, is disclosed. The system includes a measurement system, such as an ultrasonic sensor, configured to determine data characterizing the blood sample. For example, the data could be displacement of the blood sample in response to ultrasonic pulses. An integrated aspect of the system may be a common sensor, sample portion or data for fast and efficient determination of both parameters. The parameters can also be used to correct or improve measured parameters. For example, physiological adjustments may be applied to the hemostatic parameters using a HCT measurement. Also, physical adjustments may be applied, such as through calibration using a speed or attenuation of the sound pulse through or by the blood sample. These parameters may be displayed on a GUI to guide treatment.

Abstract: A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed.

Abstract: Chemical indicator apparatuses containing one or more chemical indicators for use in monitoring the quality of water in an aquatic environment. The apparatuses are designed and configured to be submersible in the water that is being monitored. In some embodiments, each apparatus includes a plurality of immobilized-dye-based chemical indicators that undergo a physical change as levels of one or more constituents of the water change. Such indicators can be read by one or more suitable optical readers. These and other embodiments are designed and configured to be movable by a corresponding monitoring/measuring apparatus, for example, via a magnetically coupled drive. Also disclosed are a variety of features that can be used to provide a chemical indicator apparatus with additional functionalities.

Abstract: A biochip including a chip body, a first electrode and a second electrode is provided. The body has a first accommodating cavity, a second accommodating cavity and a micro-fluid channel. The micro-fluid channel is connected with the first accommodating cavity and the second accommodating cavity. The first electrode has a first end and a second end. The first end is used for contacting a first probe of a detection apparatus. The second end is positioned in the first accommodating cavity. The second electrode has a third end and a forth end. The third end is used for contacting a second probe of the detection apparatus. The forth end is positioned in the second accommodating cavity.

Abstract: An apparatus for safety-compliant emptying and filling of a reagent container (24, 40) for a tissue processor (20) comprises a suction line (30, 42) for aspirating a reagent out of the reagent container (24, 40) and a delivery line (32, 44) for filling the reagent container (24, 40) with the reagent, a terminating opening (31, 43) of the suction line (30, 42) being spaced away from a terminating opening (33, 45) of the delivery line (32, 44).

Abstract: Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device.

Abstract: Provided is a method for checking a blood status including: a step of supplying blood to the centrifugal container of a disk; a step of rotating the disk to centrifuge the blood cells and blood plasma in the centrifuge container, and detecting the actual moving distance per hour of the blood cells in the centrifugal container; and a step of establishing a first graph which represents the actual moving distance of the blood cells per hour, and a second graph which represents the theoretical moving distance of the blood cells per hour, and thereafter calculating the hematocrit of the blood cells and the viscosity of the blood plasma by comparing the first graph with the second graph.