Fluorescence Patents (Class 422/82.08)
  • Patent number: 11803046
    Abstract: An apparatus and method for simultaneous imaging and execution of contact-free directed hydrodynamic flow in a specimen. The apparatus includes a laser adapted to dynamically heat the specimen, a microscope with an objective adapted to image at least a part of the specimen and to guide a light beam of the laser into and/or onto the specimen to heat at least one specified location of the specimen, means for manipulating the specified location, and a sample chamber for the specimen that is accessible for imaging radiation and the light beam to allow simultaneous imaging and manipulation of the sample via the objective.
    Type: Grant
    Filed: November 23, 2018
    Date of Patent: October 31, 2023
    Assignee: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften E.V.
    Inventors: Moritz Kreysing, Anatol Fritsch, Matthaeus Mittasch
  • Patent number: 11782006
    Abstract: A sensing system includes a charge sensor including two electrodes and an electrically conductive channel connecting the two electrodes. The sensing system also includes a charged molecule attached to the electrically conductive channel. The charged molecule includes a recognition site to reversibly bind a label of a labeled nucleotide; has an unbound favored conformation associated with an unbound charge configuration; and has a favored conformation associated with a charge configuration when the recognition site is bound to the label. The charge configuration is different from the unbound charge configuration. The sensing system further includes a polymerase attached to the electrically conductive channel or to the charged molecule.
    Type: Grant
    Filed: December 3, 2019
    Date of Patent: October 10, 2023
    Assignee: Illumina, Inc.
    Inventors: Boyan Boyanov, Sergio Peisajovich, Jeffrey G. Mandell
  • Patent number: 11692221
    Abstract: The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing.
    Type: Grant
    Filed: August 1, 2017
    Date of Patent: July 4, 2023
    Inventors: Jin Yang, Xun Xu, Hui Wang, Bin Xie, Zhuokun Li, Shengming Zhao, Ao Chen, Chongjun Xu, Wenwei Zhang, Ming Ni
  • Patent number: 11630062
    Abstract: A biosensor is provided. The biosensor includes a substrate, photodiodes, pixelated filters, an excitation light rejection layer and an immobilization layer. The substrate has pixels. The photodiodes are disposed in the substrate and correspond to one of the pixels, respectively. The pixelated filters are disposed on the substrate. The excitation light rejection layer is disposed on the pixelated filter. The immobilization layer is disposed on the excitation light rejection layer.
    Type: Grant
    Filed: October 10, 2019
    Date of Patent: April 18, 2023
    Inventors: Hsin-Yi Hsieh, Chin-Chuan Hsieh, Wei-Ko Wang, Yu-Jen Chen, Yi-Hua Chiu, Chung-Jung Hsu
  • Patent number: 11607157
    Abstract: Provided is a flexible transcutaneous oxygen partial pressure sensor which may be closely attached to a skin, be used repeatedly for a long time, and have a highly reliable measurement value. The flexible transcutaneous oxygen partial pressure sensor of the present disclosure includes: an oxygen sensing film having one surface in contact with a skin; a light detecting portion including a light emitting portion which is positioned above a surface opposite to the one surface of the oxygen sensing film and includes a micro-light emitting diode (LED (?-LED)), and a light-receiving portion which includes an organic-photodiode (OPD); and a heater portion positioned between the oxygen sensing film and the light detecting portion, and supplying thermal energy to the skin in contact with the oxygen sensing film.
    Type: Grant
    Filed: December 4, 2020
    Date of Patent: March 21, 2023
    Assignee: A-sen Company
    Inventors: Jin-woo Park, Chang-jin Lim
  • Patent number: 11548004
    Abstract: A method for producing a liquid reaction mixture containing a radioisotope, in particular, a radioactive composition, minimizes device contamination with radioactive substances and increase speed and accuracy with which droplets are mixed. The method for producing a radioactive composition includes placing at least one first droplet L1 containing a radionuclide and at least one second droplet L2 containing a labeling substance on at least two respective dimples 5 among dimples 5 on a front surface 4b of an insulating layer 4 of a liquid manipulation device 1, and obtaining a liquid mixture M by using a change in electrostatic force caused by changing voltage applied to the electrodes 3 to thereby cause a relative movement between the at least one first droplet L1 and the at least one second droplet L2 so that the at least one first droplet L1 and the at least one second droplet L2 are mixed together at any one dimple among the dimples 5.
    Type: Grant
    Filed: May 11, 2021
    Date of Patent: January 10, 2023
    Assignees: National Institute of Advanced Industrial Science and Technology, Kyoto Pharmaceutical University
    Inventors: Katsuo Mogi, Tohru Natsume, Shungo Adachi, Tomoya Inoue, Hiroyuki Kimura
  • Patent number: 11364495
    Abstract: The present invention provides methods and devices for simple, low power, automated processing of biological samples through multiple sample preparation and assay steps. The methods and devices described facilitate the point-of-care implementation of complex diagnostic assays in equipment-free, non-laboratory settings. The invention includes a microfluidic device comprising a reagent-dispensing unit, a sample extraction device and a specimen processing unit.
    Type: Grant
    Filed: December 1, 2017
    Date of Patent: June 21, 2022
    Inventors: Andrea Maria Dominic Pais, Rohan Joseph Alexander Pais
  • Patent number: 11360026
    Abstract: A fluorescence immunoassay device based on integration of a photonic crystal and magnetic beads and a method thereof are provided. Magnetic beads with high surface-to-volume ratio are used as carriers of fluorescent molecules to obtain higher fluorescence density. The electric field on the surface of the photonic crystal is enhanced through excitation of photonic crystal resonance. The intensity of the fluorescence signal excited by the enhanced electric field is increased. Moreover, through interaction with the photonic crystal, some fluorescent signals that originally cannot be received by the fluorescent sensor are coupled to the photonic crystal resonant modes and reradiate toward the fluorescent sensor, thereby increasing collection efficiency. The fluorescence signals generated by fluorescent molecules on the magnetic beads are significantly intensified, which could lower the detection limit.
    Type: Grant
    Filed: April 17, 2018
    Date of Patent: June 14, 2022
    Assignee: National Yang Ming Chiao Tung University
    Inventors: Cheng-Sheng Huang, Wen-Syang Hsu, Lin-Yun Su, Ying-Bin Wang, Yang Chen
  • Patent number: 11208682
    Abstract: Disclosed herein are improved methods and systems for sequencing nucleic acid that exploit the temperature-dependence of the emitted intensity of fluorescent dyes. The temperature of the sequencing reaction is adjusted during each sequencing cycle, and the emission, or lack of emission, of light meeting or exceeding a threshold by the fluorescent dyes at different temperatures, or within different temperature ranges, is used to detect the fluorescent labels of the incorporated dNTPs and thereby sequence the nucleic acid. The disclosed methods enable a determination of the dNTP incorporated at any given site with a reasonable number of chemistry steps without the complex optics necessary for prior-art systems.
    Type: Grant
    Filed: September 13, 2019
    Date of Patent: December 28, 2021
    Assignee: Western Digital Technologies, Inc.
    Inventors: Patrick Braganca, Daniel Bedau
  • Patent number: 11185837
    Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
    Type: Grant
    Filed: August 8, 2019
    Date of Patent: November 30, 2021
    Assignee: Twist Bioscience Corporation
    Inventors: William Banyai, Bill James Peck, Andres Fernandez, Siyuan Chen, Pierre Indermuhle
  • Patent number: 11131632
    Abstract: A measuring device for the measurement of light signals emitted from samples in wells of a microplate, in particular to a luminescence measuring device for measuring luminescence signals. The luminescence measuring device has a first injector with an injector needle having an outlet end for dispensing a reagent into a well, and a luminescence optical device having an optical axis extending substantially perpendicularly to the microplate and a measuring region surrounding the optical axis. The injector needle extends to below the luminescence optical device and opens with its outlet end into the measuring region. The luminescence measuring device has further a spray deflector arranged between the outlet end of the injector and the luminescence optical device.
    Type: Grant
    Filed: August 17, 2017
    Date of Patent: September 28, 2021
    Assignee: TECAN TRADING AG
    Inventors: Manfred Lansing, Johannes Posch, Tobias Sawetzki
  • Patent number: 11009462
    Abstract: Disclosed are calibration apparatuses for fluorescent microscopy instruments and methods of making and using them. Specifically, disclosed are calibration apparatuses with a fluorescent layer, such as photoresist, deposited on a substrate, with an optional layer of a reflective material, such as chrome. Illumination of the fluorescent and/or reflective layers, and detection and analysis of the resulting emissions allows evaluation of the instrument with respect to both reflective and fluorescent channels. Selection of appropriate fluorescent materials for the one or more fluorescent layers allows the evaluation of an instrument with respect to different fluorophores, as would be used with an instrument capable of two color detection.
    Type: Grant
    Filed: August 13, 2019
    Date of Patent: May 18, 2021
    Assignee: Affymetrix, Inc.
    Inventors: Bei-Shen Sywe, Mark Borodkin, Chuan Gao, Liana Ilkova, Devin Nguyen
  • Patent number: 10967338
    Abstract: A system for analyzing a biological sample may include at least one sample acquisition stage comprising a sample acquisition device for acquiring the biological sample from a sample source; a droplet generator device for forming a droplet wrapped in an immiscible carrier fluid, wherein the wrapped droplet comprises at least the biological sample and a reagent, the droplet generator configured to receive the biological sample transferred from the sample acquisition device; a collection vessel for collecting the wrapped sample droplet from the droplet generator, the vessel configured to contain a carrier fluid for receiving and protecting the sample droplet; and an analysis system for analyzing the wrapped sample droplet and detecting products of a polymerase chain reaction.
    Type: Grant
    Filed: October 9, 2018
    Date of Patent: April 6, 2021
    Assignee: STOKES BIO LTD.
    Inventors: Mark Davies, Tara Dalton
  • Patent number: 10921098
    Abstract: An explosive detection system may include a suction nozzle having a suction port for introducing air containing explosive particles at one end thereof, a discharge nozzle having a discharge port for discharging air at one end thereof, a sensing block in which a detection material capable of detecting explosive particles in the air is disposed, a sensor unit for emitting light to the sensing block and outputting a sensing signal, a first guide pipe connected to the other end of the suction nozzle and guiding the air introduced through the suction nozzle to the sensing block in which the detection material is disposed, a suction force generating unit formed at the other end of the discharge nozzle and to suck air through the suction port and to provide a suction force for sucking air into the sensing block and discharging the air to a discharge port formed at one end of the discharge nozzle, a second guide pipe formed between the sensing block and the suction force generating unit and discharging the air introd
    Type: Grant
    Filed: August 24, 2018
    Date of Patent: February 16, 2021
    Assignee: ISENTECH Co., Ltd.
    Inventors: Inchang Park, Jong Hyun Kim
  • Patent number: 10823661
    Abstract: A sensor for isolating, identifying, and quantifying one or more analytes in a sample is provided. Also provided are methods of isolating, identifying, and quantifying one or more analytes in a sample, the method including contacting a polymer waveguide with a sample, sequentially heating the polymer waveguide to a plurality of temperature thresholds, obtaining an optical output at each temperature threshold, and analyzing differences in sequentially-obtained optical outputs in order to identify and determine concentrations of individual analytes of interest in the sample.
    Type: Grant
    Filed: August 18, 2016
    Date of Patent: November 3, 2020
    Assignee: University of Cincinnati
    Inventors: Fred R. Beyette, Jr., Geethanga Gayan De Silva
  • Patent number: 10743529
    Abstract: The inventive subject matter provides a method for the incorporation of colored photoluminescent materials to be sprayed on to plants and soils. The incorporation of colored photoluminescent materials assists the spray operator through the use of black lights allowing for more precise low light or nighttime spray and seeding operations. More specifically, this invention relates to a colored photoluminescent adjuvant to be applied to targeted crops, plants and seeds to assist in the low light or nighttime application of bioactive agents, such as chemicals and pesticides. The colored photoluminescent adjuvant can be applied as a solution or a foam.
    Type: Grant
    Filed: November 16, 2017
    Date of Patent: August 18, 2020
    Assignee: Floratine Products Group, Inc.
    Inventors: Kevin Cavanaugh, Timothy B. Cartwright
  • Patent number: 10730891
    Abstract: The present invention relates to a compound (L) for selective determination of free cysteine and a process for the preparation thereof. wherein R1 is selected from benzene, toluene, naphthalene and pyrene.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: August 4, 2020
    Inventors: Amitava Das, Firoj Ali, Upendar Reddy Gandra, Anila Hoskere Ashok, Samit Chattopadhyay, Nandaraj Taye
  • Patent number: 10711294
    Abstract: The substrate 100 for use in the analysis of a nucleic acid according to the present invention has multiple analysis areas 12 which are partitioned on a substrate 10, and enables the measurement of the analysis areas 12 while interchanging the analysis areas 12 in turn, said substrate 100 being characterized in that each of the analysis areas 12 consists of an adsorption part 13 onto which a DNA fragment or a carrier having the DNA fragment carried thereon can be adsorbed and a non-adsorption part 14 which is a part outside of the adsorption part 13, and the non-adsorption part 14 has, formed on at least a part thereof, a marker part 15 that has a specified shape and helps to identify the positions of the analysis areas 12.
    Type: Grant
    Filed: December 26, 2014
    Date of Patent: July 14, 2020
    Inventors: Yuichiro Ota, Tomohiro Shoji, Toru Yokoyama, Masatoshi Narahara
  • Patent number: 10690689
    Abstract: A microfluidic sequencing device in which a stream of microdroplets at least some of which contain a single nucleotide base are made to undergo reaction with a capture system to capture and detect an ordered sequence of single nucleotide bases generated by progressive pyrophosphorolysis.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: June 23, 2020
    Inventors: Cameron Alexander Frayling, Barnaby Balmforth, Bruno Flavio Nogueira de Sousa Soares, Thomas Henry Isaac, Boris Breiner, Alessandra Natale, Michele Amasio, Paul Dear
  • Patent number: 10690596
    Abstract: The surface plasmon-enhanced fluorescence measurement device has: a light source that irradiates the diffraction grating with a linearly polarized excitation light; a rotating part that changes the direction of the optical axis of the excitation light with respect to the diffraction grating when seen in plan view, or changes the polarization direction of the excitation light with respect to the diffraction grating; a polarizer that extracts linearly polarized light from the fluorescence emitted from the fluorescent substance; and a light detection unit that detects the linearly polarized light extracted by the polarizer.
    Type: Grant
    Filed: December 1, 2015
    Date of Patent: June 23, 2020
    Assignee: Konica Minolta, Inc.
    Inventors: Yukito Nakamura, Kosuke Nagae, Takatoshi Kaya
  • Patent number: 10647873
    Abstract: Provided herein is a polymerizable liquid composition useful for additive manufacturing, which composition may include: (i) a free radical photoinitiator; (ii) monomers and/or prepolymers (e.g., reactive diluents) that are polymerizable by exposure to actinic radiation or light, optionally wherein some or all of said monomers and/or prepolymers comprise one or more acid-labile groups; (iii) a chain extender, optionally wherein said chain extender comprises one or more acid-labile groups; and (iv) a photoacid generator, wherein at least one of said monomers and/or prepolymers, or said chain extender, comprises one or more acid-labile groups, and wherein said free radical photoinitiator and said photoacid generator are, or are selected to be, activated by light at different ranges of wavelengths or intensities. Methods of forming a three-dimensional object with the composition, and articles so formed are also provided.
    Type: Grant
    Filed: October 28, 2016
    Date of Patent: May 12, 2020
    Assignee: Carbon, Inc.
    Inventors: Jason P. Rolland, Nordstrom Kirk Phelps, Ariel M. Herrmann
  • Patent number: 10626457
    Abstract: Arrays of integrated optical devices and their methods for production are provided. The devices include an integrated bandpass filter layer that comprises at least two multi-cavity filter elements with different central bandpass wavelengths. The device arrays are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices provide for the efficient and reliable coupling of optical excitation energy from an optical source to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination. The device arrays are well suited for miniaturization and high throughput.
    Type: Grant
    Filed: October 28, 2016
    Date of Patent: April 21, 2020
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Ravi Saxena, Annette Grot, Nicolaas Tack, Pilar Gonzalez, Bert Du Bois, Simone Severi
  • Patent number: 10591415
    Abstract: The present disclosure a biochip including a side emitting-type light-emitting device, in which the bio-chip includes: a light-emitting device for emitting light from a fluorescent material; reflective layers provided over and under the light-emitting device so as to emit light from the sides of the light-emitting device; and reaction regions formed by etching of flanking regions of the light-emitting device. In the biochip, light emitted from the sides of the light-emitting device causes a biochemical reaction in the reaction regions. According to the present disclosure, light emitted from the light-emitting device moves only laterally without being transferred to the top or bottom of the bio-layer, and is transferred to the reaction regions formed by etching of flanking regions of the light-emitting device, so that a biochemical reaction in the reaction regions can be more efficiently performed.
    Type: Grant
    Filed: June 6, 2018
    Date of Patent: March 17, 2020
    Inventors: Do Young Lee, In Gyun Jeon
  • Patent number: 10545516
    Abstract: Automated measurement of fluid solution capacitance in industrial processes to determine solution concentration. Industrial process control transmitters determine solution concentration directly from solution capacitance and confirm concentration determinations based on solution conductivity. The industrial process control transmitters include terminals embodied in wire coils and/or metallic plates, at least one processor, and at least one computer readable memory device.
    Type: Grant
    Filed: August 2, 2017
    Date of Patent: January 28, 2020
    Assignee: Schneider Electric Systems USA, Inc.
    Inventor: James H. Grassi
  • Patent number: 10544413
    Abstract: Methods and systems for sorting droplets are provided. In some cases, occupied droplets may be sorted from unoccupied droplets. In some cases, singularly occupied droplets may be sorted from unoccupied droplets and multiply occupied droplets. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads. Methods and systems for selectively polymerizing droplets based on occupancy and size of the droplets are provided.
    Type: Grant
    Filed: July 10, 2018
    Date of Patent: January 28, 2020
    Assignee: 10X Genomics, Inc.
    Inventors: Rajiv Bharadwaj, Michael Schnall-Levin, Anthony Makarewicz, Steven Short
  • Patent number: 10501796
    Abstract: An apparatus can include a vessel, a reference surface, a preload, a scan actuator, and a transmitter. The reference surface can form a structural loop with a detector. The preload can be configured to urge the vessel to contact an area on the reference surface. The scan actuator can be configured to slide the vessel along the reference surface in a scan dimension. The transmitter can be configured to direct signal from the vessel to a detector and/or direct energy from an energy source to the vessel, when the vessel is urged by the preload to contact the reference surface.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: December 10, 2019
    Inventors: Dale Buermann, Michael John Erickstad, Rebecca McGinley, Alex Nemiroski, Harry Scott Rapoport, Arnold Oliphant
  • Patent number: 10504688
    Abstract: An analytical cell includes a first substrate and a second substrate each having a through hole extending in a thickness direction thereof. The first substrate and the second substrate are partially overlapped with each other to form an overlapping portion. In the overlapping portion, a solid state joint is formed by solid state bonding of a first solid portion protruding from the first substrate and a second solid portion protruding from the second substrate, whereby the first substrate and the second substrate are spaced from each other by a predetermined distance, and joined together in a state where the first substrate and the second substrate are positioned to form an observation window. At the observation window, the through holes of the first substrate and the second substrate face each other, and an electron beam is transmitted through the observation window.
    Type: Grant
    Filed: June 28, 2016
    Date of Patent: December 10, 2019
    Assignee: Honda Motor Co., Ltd.
    Inventors: Takanori Maebashi, Nariaki Kuriyama, Yoshiya Fujiwara
  • Patent number: 10451549
    Abstract: The present invention relates to an optical fiber for use in a system for detection of one or more compounds in a fluid.
    Type: Grant
    Filed: May 16, 2016
    Date of Patent: October 22, 2019
    Inventors: Diana Viegas, Raquel Queirós, Jana Nieder, Maria Teresa Férnandez, Begoña Espiña, Paolo Freitas, Lars Montelius
  • Patent number: 10443083
    Abstract: A biological indicator analyzer includes a plurality of wells, a plurality of organism detector features, and a user input feature such as a touch screen. Each well is configured to receive a respective biological indicator. Each organism detector feature is configured to detect whether a biological indicator disposed in a corresponding well of the plurality of wells contains a living organism. The touch screen is configured to receive user input and provide information to the user indicating a status of biological indicator analysis. The biological indicator analyzer may be used to analyze a biological indicator that was positioned in a sterilization chamber of a sterilizing cabinet along with at least one medical device that is to be sterilized. The analysis may indicate whether the sterilization cycle in the sterilization chamber as successful.
    Type: Grant
    Filed: February 24, 2017
    Date of Patent: October 15, 2019
    Assignee: ASP Global Manufacturing GmbH
    Inventors: Darius D. Eghbal, Jacob S. Childs, Margaret D. Shaffer, Jeremy M. Yarwood, Benjamin M. Fryer, Howell B. Schwartz
  • Patent number: 10393613
    Abstract: An analysis system includes a fluidic system includes a number of components that are interconnected to form a fluidic system having a plurality of flow paths. An example method of pressure testing the fluidic system includes (a) selecting a flow path from the plurality of flow paths through a flow cell in accordance with a prescribed test protocol; (b) actuating a pump to pressurize a fluid in the selected flow path; (c) generating pressure data representative of the pressure in the selected flow path; and (d) processing the pressure data to determine whether the selected flow path maintains pressure in a desired manner.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: August 27, 2019
    Assignee: ILLUMINA, INC.
    Inventors: Bradley Kent Drews, Kevin James Cappa
  • Patent number: 10301676
    Abstract: A nucleic acid sequencing system is provided, including a base provided with a clamping platform, a reagent storage unit, a fluid control unit, a mobile platform and a total reflection microscope thereon; the clamping platform being provided with a gene sequencing chip thereon; the reagent storage unit being configured to store a gene sequencing reagent, the fluid control unit being configured to pump the gene sequencing reagent from the reagent storage unit to the gene sequencing chip, the mobile platform being configured to drive the clamping platform to move toward or away from the total reflection microscope; and the total reflection microscope being configured to detect a gene sequence of a sample in the gene sequencing chip.
    Type: Grant
    Filed: June 2, 2017
    Date of Patent: May 28, 2019
    Inventors: Qin Yan, Zefei Jiang, Jiao Zheng, Ping Wu, Zhiliang Zhou, Liangjin Ge
  • Patent number: 10267733
    Abstract: The present disclosure relates to semiconductor devices for detecting fluorescent particles. At least one embodiment relates to an integrated semiconductor device for detecting fluorescent tags. The device includes a first layer, a second layer, a third layer, a fourth layer, and a fifth layer. The first layer includes a detector element. The second layer includes a rejection filter. The third layer is fabricated from dielectric material. The fourth layer is an optical waveguide configured and positioned such that a top surface of the fourth layer is illuminated with an evanescent tail of excitation light guided by the optical waveguide when the fluorescent tags are present. The fifth layer includes a microfluidic channel. The optical waveguide is configured and positioned such that the microfluidic channel is illuminated with the evanescent tail. The detector element is positioned such that light from activated fluorescent tags can be received.
    Type: Grant
    Filed: May 22, 2015
    Date of Patent: April 23, 2019
    Assignee: IMEC VZW
    Inventors: Pol Van Dorpe, Liesbet Lagae, Peter Peumans, Andim Stassen, Philippe Helin, Bert Du Bois, Simone Severi
  • Patent number: 10130949
    Abstract: The present invention concerns a microfluidic system comprising: a microchannel containing several elements of two non-miscible fluids, the microchannel comprising a droplet (30) containing magnetic particles (M), and a device for generating inside the microchannel magnetic field, said device comprising an activable magnetic element, the activable magnetic element comprising a tip (5,6), the microfluidic system being configured to transport the droplet by flow or by pressure difference.
    Type: Grant
    Filed: July 24, 2012
    Date of Patent: November 20, 2018
    Inventors: Jean-Louis Viovy, Laurent Malaquin, Stefano Begolo, Anaïs Ali Cherif, Stephanie Descroix
  • Patent number: 10013835
    Abstract: The invention relates to a security feature for safeguarding value documents, comprising particles which are composed of at least two different, spatially separate solid homogeneous phases, wherein each of the phases is detectable and identifiable in a spatially resolved manner by means of a spatially resolving analytical method that is adapted for resolving down to the single-particle level.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: July 3, 2018
    Inventors: Johann Kecht, Martin Stark
  • Patent number: 9977016
    Abstract: Methods of characterizing nanoparticles, nanoparticle complexes, and their biomolecular interactions are provided. Concurrent excitation and emission wavelength scans are performed and the optimal fluorescence intensity for the intersect of these wavelengths is determined. The intersect is dependent upon the physical characteristics of the particle and can be used to verify, for example, attachment of biomolecules, amount of biomolecules present, and structure of the attached biomolecule.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: May 22, 2018
    Assignee: Kansas State University Research Foundation
    Inventors: Robert DeLong, Miranda Hurst
  • Patent number: 9945780
    Abstract: A System and method for self-checking a fluorometer for failure or deteriorated performance includes fluorescent reference standards mounted on a support to move with respect to one or more fixed fluorometers. The intensity of the fluorescent emission of the fluorescent reference standard is initially measured with the fluorometer, and, after a prescribed interval of usage of the fluorometer, a test measurement of the intensity of the fluorescent emission of the fluorescent standard is taken with the fluorometer. The test measurement is compared to the initial measurement, and failure or deteriorated performance of the fluorometer is determined based on a deviation of the test measurement from the initial measurement.
    Type: Grant
    Filed: June 7, 2013
    Date of Patent: April 17, 2018
    Inventors: Haitao Li, David Opalsky, R. Eric Heinz, Norbert D. Hagen
  • Patent number: 9903805
    Abstract: A polarimeter for measuring chirality of a material comprising an optical ring cavity comprising a plurality of reflective elements configured to promote bi-directional propagation of a laser beam within the cavity, a laser-emitting device configured to introduce a first input beam and a second input beam into the cavity, a Faraday rotator, a phase compensator, an acousto-optic modulator configured to shift the frequency of the first output beam, an optical recombination device configured to recombine shifted and unshifted output beams, and first and second detectors configured to receive the recombined beams, wherein the plurality of reflective elements, Faraday rotator, and phase compensator are configured such that light from the first and second input beams passes through a chiral material located within the cavity a sufficient number of times for a measurement of optical rotary dispersion and circular dichroism of light transmitted through the material to be obtained at the detectors.
    Type: Grant
    Filed: July 11, 2017
    Date of Patent: February 27, 2018
    Inventor: Theodore Peter Rakitzis
  • Patent number: 9869664
    Abstract: The present invention relates to a method and apparatus for determining trace amounts of water in oil, including the steps of obtaining a blended sample of a low water content fluid including an amount of water and an amount of oil, transferring a portion of the blended sample to a cuvette to create a measurement sample, covering a viewing surface of the cuvette with an opaque sheet with a slit, providing a barrier with a fixed opening between a laser source and a laser-receptive surface of the cuvette, transmitting a pulsed laser beam from the laser source into the cuvette; inducing fluorescence in the measurement sample, focusing the fluorescence through a collecting lens and transmitting the fluorescence to a spectrometer, and measuring the fluorescence with the spectrometer by dispersing the fluorescence with a spectrograph and intensifying the fluorescence with an intensified charge coupled device.
    Type: Grant
    Filed: December 18, 2013
    Date of Patent: January 16, 2018
    Inventors: Ezzat M. Hegazi, Abdul Rahman Zafer Akhras
  • Patent number: 9745625
    Abstract: Methods and products for analyzing polymers are provided. The methods include methods for determining various other structural properties of the polymers.
    Type: Grant
    Filed: September 23, 2013
    Date of Patent: August 29, 2017
    Inventor: Eugene Y. Chan
  • Patent number: 9677999
    Abstract: A system for increasing light throughput in cavity enhanced spectrometry, and a model for cavity enhanced absorption measurements are presented. The cavity has an entrance mirror, an opposed exit mirror and a detector positioned adjacent the exit mirror. An input aperture is defined in the entrance mirror to allow light from a source to enter the cavity. The input aperture improves light throughput without significant departure from the theoretically predicted amplification of absorbance. This results in improvement of detection limits, even with mirrors of modest reflectivity and inexpensive detectors.
    Type: Grant
    Filed: March 26, 2015
    Date of Patent: June 13, 2017
    Inventor: Purnendu K Dasgupta
  • Patent number: 9618457
    Abstract: A chemical sensor according to an embodiment of the present technology includes a substrate, a low refractive index layer, a high refractive index layer, and a light detection unit. The low refractive index layer is laminated on the substrate and has a second refractive index smaller than a first refractive index that is a refractive index of a detection target object. The high refractive index layer is laminated on the low refractive index layer, has a third refractive index larger than the first refractive index, and includes a holding surface on which the detection target object is held, and illumination light propagates therein. The light detection unit is provided on the substrate and detects detection target light generated from the detection target object by the illumination light.
    Type: Grant
    Filed: October 26, 2012
    Date of Patent: April 11, 2017
    Inventors: Masanori Iwasaki, Nobuyuki Matsuzawa, Kensaku Maeda, Yusuke Moriya
  • Patent number: 9599569
    Abstract: Methodology for determining optical functions of thin films with enhanced sensitivity to “p” polarized electromagnetic radiation reflected from both interfaces of an absorbing film.
    Type: Grant
    Filed: April 28, 2016
    Date of Patent: March 21, 2017
    Assignee: J.A. WOOLLAM CO., INC.
    Inventors: Thomas E. Tiwald, Jeremy A. Van Derslice
  • Patent number: 9594005
    Abstract: A fluorescence detection assembly that includes an emitter, a detector, a housing that defines an light chamber, a fluorescence chamber and a well, a light path that extends from the emitter, through the light chamber and through the well, and a fluorescence path that extends from the well, through the fluorescence chamber and to the detector.
    Type: Grant
    Filed: June 29, 2015
    Date of Patent: March 14, 2017
    Assignee: Pulse Health LLC
    Inventors: David Barsic, Rachel Dreilinger, Martin Krauss, Jon Boro, James Ingle
  • Patent number: 9594296
    Abstract: An illumination device includes an excitation light source which emits excitation light, a wavelength converter which generates fluorescent light having a wavelength different from that of the excitation light through the excitation of the excitation light and a light path-splitting member including a first filter and a second filter arranged to alternately come across a light path of the excitation light, wherein the first filter reflects one of the excitation light and the fluorescent light and transmits the other of the excitation light and the fluorescent light, the second filter transmits light reflected by the first filter and reflects light transmitted through the first filter, and the wavelength converter is disposed in a reflection light path or a transmission light path of the excitation light.
    Type: Grant
    Filed: September 12, 2013
    Date of Patent: March 14, 2017
    Assignee: RICOH COMPANY, LTD.
    Inventors: Kazuhiro Fujita, Tatsuya Takahashi, Toshiharu Murai, Ikuo Maeda, Takehiro Nishimori
  • Patent number: 9546247
    Abstract: Explosives detection polymers are provided that include a polyamine polymer, such as polyethylenimine, functionalized with a small molecule fluorophore. Methods for detecting an explosive material using polyamine polymers functionalized with a small molecule fluorophore are also provided. Sensors for explosive detection are provided that include a polyamine polymer functionalized with a small molecule fluorophore and a complementary analytical device.
    Type: Grant
    Filed: March 21, 2013
    Date of Patent: January 17, 2017
    Assignee: University of Connecticut
    Inventors: Yu Lei, Ying Wang
  • Patent number: 9541561
    Abstract: Provided is a method for diagnosing Alzheimer/s disease using a decomposed biomaterial. The method includes: preparing magnetic particles on which primary capture antibodies specifically bound to beta-amyloid are adsorbed; decomposing a biomaterial including beta-amyloid; binding the beta-amyloid to the primary capture antibodies; binding secondary capture antibodies labeled with a fluorescent material to the magnetic particles bound to the beta-amyloid to form a complex; disposing the complex in a channel region of a photo field effect transistor in which a photocurrent is changed according to the amount of incident light; and measuring the photocurrent changed by light excited from the complex to quantify the concentration of the beta-amyloid existing in the biomaterial.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: January 10, 2017
    Inventors: Chang-Beom Kim, Kibong Song, Kwan Su Kim
  • Patent number: 9458451
    Abstract: A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample.
    Type: Grant
    Filed: June 20, 2008
    Date of Patent: October 4, 2016
    Inventors: Robert E. Heinz, Haitao Li
  • Patent number: 9423378
    Abstract: Methods for the detection of biologically relevant molecules that comprise concentrating such molecules into microscopic holes in a sheet of chemically inert material, restricting the openings, and measuring the electric current through the holes or the fluorescence near the hole openings. The electric current or fluorescence will change as the molecules diffuse out of the holes, providing a measure of the diffusion rate and thereby detecting the presence and characteristics of the molecules. For molecules that interact, the diffusion rate will be slower than for molecules that do not interact, yielding a determination of the molecular interaction. Capping the population of holes and inserting into a mass spectrometer allows identification of the molecules.
    Type: Grant
    Filed: March 4, 2014
    Date of Patent: August 23, 2016
    Inventor: Christopher Gordon Atwood
  • Patent number: 9372188
    Abstract: An integrated microelectronic sensor is provided in a disposable flow membrane sensing device. The integrated sensors detect electromagnetic effect labels in flow detection zones above the sensor in the membrane. The labels are small particles that give off a detectable electromagnetic signal. They are commonly used for isolating and quantifying biochemical targets of interest. The sensors are fabricated using planar integrated circuit technologies. Sensors can detect labels of several types including magnetic, electric, and photonic. These types all have in common the fact that the sensor detects the label at a distance. Magnetoresistive sensors for detecting magnetic labels, and photodiodes for detecting photonic labels are described. A system for using the sensors is described. There are disposable cartridges with a backing that supports the sensors and membrane is described. The integrated sensor in the cartridge is designed to be discarded after use. Also, label excitation sources are provided.
    Type: Grant
    Filed: April 30, 2014
    Date of Patent: June 21, 2016
    Inventor: Mark Christopher Tondra
  • Patent number: 9346997
    Abstract: A nanostructured particulates formed from a redox active, luminescent phenyl substituted polycyclic aromatic hydrocarbon are provided herein. The nanostructured particulates may be used for determining the presence of an analyte of interest in a sample by detecting the emitted electromagnetic radiation generated by exposing a reagent mixture, which includes the nanostructured particulates and the sample, to electrochemical energy.
    Type: Grant
    Filed: July 9, 2014
    Date of Patent: May 24, 2016
    Assignee: Board of Regents of the University of Texas System
    Inventors: Allen J. Bard, Fu-Ren F. Fan, Khalid Omer