Monosaccharide Patents (Class 435/105)
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Publication number: 20110217740Abstract: Disclosed herein are methods of degrading plant biomass, and microorganisms and polypeptides used in such methods, hi certain embodiments, the methods include growing Anaerocellum thermophilum on a substrate that comprises plant biomass under conditions effective for the A. thermophilum to convert at least a portion of the plant biomass to a water soluble product or a water insoluble product, hi some cases, the method can further include one or more steps to further process the water soluble product or a water insoluble product to produce, for example, a biofuel or commodity chemical. In another aspect, microorganisms that include at least one A. thermophilum plant biomass utilization polynucleotide are disclosed. Also disclosed are methods of transferring one or more A. thermophilum plant biomass utilization polynucleotides to a recipient microorganism. A. thermophilum plant biomass utilization polynucleotides and polypeptides encoded by such polynucleotides are also disclosed.Type: ApplicationFiled: August 26, 2009Publication date: September 8, 2011Applicant: University of Georgia ResearchInventors: Michael W.W. Adams, Janet Westpheling, Scott Hamilton-Brehm, Irina Kataeva, Sung-Jae Yang, Farris Poole
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Patent number: 8012721Abstract: The present invention pertains to a method of converting cellulose to glucose by treating a pretreated lignocellulosic substrate with an enzyme mixture comprising cellulase enzyme and endoglucanase core proteins, wherein the endoglucanase core proteins are present in the enzyme mixture at an amount relative to all endoglucanases from about 35 wt. % to about 100 wt. % and wherein the endoglucanase cellulase enzymes are present in the enzyme mixture at an amount relative to the amount of CBH and EG enzymes from about 2 wt. % to about 50 wt. %. The pretreated lignocellulosic substrate is selected from the group consisting of agricultural residues, residues after starch or sugar removal, dedicated ethanol crops, forestry products, and pulp and paper products, or combinations thereof.Type: GrantFiled: March 5, 2003Date of Patent: September 6, 2011Assignee: Iogen Energy CorporationInventors: Daphne Wahnon, Theresa C. White, Jennifer Donaldson, Jeffrey S. Tolan
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Publication number: 20110212458Abstract: The invention provides biosynthetic routes to xylitol production that do not require pure D-xylose for synthesis and that can utilize inexpensive substrates such as hemicellulose hydrolysates.Type: ApplicationFiled: May 11, 2011Publication date: September 1, 2011Inventors: Paul Taylor, Ian Fotheringham, Nathan Wymer, Badal Saha, David Demirjian, Yoshikiyo Sakaibara, Francis Michael Racine
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Patent number: 8008056Abstract: Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.Type: GrantFiled: December 22, 2005Date of Patent: August 30, 2011Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Frits Goedegebuur, Lydia Dankmeyer, Colin Mitchinson, Paulien Neefe, Brad Kelemen, Robert Caldwell, Pauline Teunissen
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Publication number: 20110201060Abstract: This invention pertains to a process for manufacturing scyllo-Inositol. Specifically, the current invention pertains to a process for converting myo-Inositol to scyllo-Inositol using a bioconversion process.Type: ApplicationFiled: February 14, 2011Publication date: August 18, 2011Applicant: Abbott LaboratoriesInventors: Rajarathnam E. Reddy, Sanjay R. Chemburkar, Douglas R. Spaulding, Yi Pan, Lei Cao, Jose A. Restituyo, Richard Lorenzini, Michael DeMarco
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Patent number: 7998711Abstract: Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.Type: GrantFiled: July 6, 2007Date of Patent: August 16, 2011Assignee: Danisco US Inc.Inventors: Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Edmund Larenas
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Patent number: 7998709Abstract: The present invention relates to a process for enzymatic hydrolysis of granular starch into a soluble starch hydrolysate at a temperature below the initial gelatinization temperature of said granular starch.Type: GrantFiled: August 13, 2009Date of Patent: August 16, 2011Assignee: Novozymes A/SInventors: Anders Viksoe-Nielsen, Carsten Andersen, Sven Pedersen, Carsten Hjort
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Publication number: 20110195464Abstract: The invention relates to processes and biocatalysts for producing ethanol and other useful products from biomass and/or other materials. Initial processing of lignocellulosic biomass frequently yields methylglucuronoxylose (MeGAX) and related products which are resistant to further processing by common biocatalysts. Strains of Enterobacter asburiae are shown to be useful in bioprocessing of MeGAX and other materials into useful bioproducts such as ethanol, acetate, lactate, and many others. Genetic engineering may be used to enhance production of desired bioproducts.Type: ApplicationFiled: November 17, 2009Publication date: August 11, 2011Applicant: University of Florida Research Foundation, Inc.Inventor: James F. Preston
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Publication number: 20110195463Abstract: A novel thermophilic endo-glucanase, nucleic acid encoding the endo-glucase, and uses thereof in converting ligocellulosic material to fermentable sugars.Type: ApplicationFiled: April 19, 2011Publication date: August 11, 2011Applicant: Academia SinicaInventors: Tuan-Hua David Ho, Su-May Yu, I Son Ng, Chii-Gong Tong
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Patent number: 7993884Abstract: Xylose-containing plant material may be hydrolyzed to xylose using a ?-D-xylosidase which exhibits unexpectedly high activity. The enzyme has a kcat value for catalysis of approximately 185 sec?1 for 1,4-?-D-xylobiose (X2) when measured at a pH of 5.3 and a temperature of 25° C.; this is at least 10-fold greater than reported for other xylosidases at 25° C. and their optimal pH. The enzyme also has an isoelectric point of approximately 4.4. When reacted at a pH between about 4.5 and about 7.7, the ?-D-xylosidase exhibits surprisingly high activity for hydrolyzing xylose-containing plant materials to xylose. The xylose released from plant materials may then be converted to other secondary products such as ethanol by fermentation or other reaction. This ?-D-xylosidase may be used alone or in combination with other hydrolytic or xylanolytic enzymes for treatment of lignocellulosic or hemicellulosic plant materials or plant material hydrolysates or xylooligosaccharides.Type: GrantFiled: September 27, 2007Date of Patent: August 9, 2011Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Douglas B. Jordan, Xin Liang Li, Christopher A. Dunlap, Terence R. Whitehead, Michael A. Cotta
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Publication number: 20110183381Abstract: Thermostable cellulase enzyme systems comprising at least one each of a thermostable endoglucanase, an exo-processive-endoglucanase, and a ?-glucosidase carry out the complete, coordinated hydrolysis of crystalline cellulose to monomeric glucose.Type: ApplicationFiled: July 9, 2009Publication date: July 28, 2011Applicant: THE BOARD OF REGENTS FOR OKLAHOMA STATE UNIVERSITYInventors: Rolf A. Prade, Hongliang Wang
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Publication number: 20110177565Abstract: An isolated polypeptide is disclosed that improves the hydrolyzing capacity of a polysaccharide hydrolase such as cellulase, is capable of binding to a polysaccharide, is deficient in polysaccharide hydrolase activity, and includes a GH45 or a pollen-allergen domain. Methods of hydrolyzing polysaccharides using the isolated polypeptide are also disclosed.Type: ApplicationFiled: September 2, 2010Publication date: July 21, 2011Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventors: Hwa Young CHO, Hyun Min KOO, Jae Chan PARK, Sung Min PARK, In Geol CHOI, Kyoung Heon KIM
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Publication number: 20110171674Abstract: The present invention relates to the expression and optimization of enzymes involved in lignocellulosic biomass decomposition. The present invention relates more particularly to beta-glucosidase variants comprising at least one modification among the amino acids located at positions 225, 238, 240 and 241, according to the numbering in SEQ ID No. 2 of Trichoderma reesei beta-glucosidase, and also relates to the use of said variants having improved effectiveness in methods for cellulose decomposition and biofuel production.Type: ApplicationFiled: September 10, 2009Publication date: July 14, 2011Applicant: IFP Energies NouvellesInventors: Nicolas Lopes-Ferreira, Antoine Margeot, Hugues Mathis, Laurent Fourage
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Publication number: 20110165631Abstract: Stable and active arabinitol dehydrogenases (LAD) from Neurospora crassa and mutants thereof are disclosed. Arabinitol dehydrogenases are useful in the production of xylitol and ethanol from an arabinose containing substrate. Recombinant and heterologously expressed arabinitol dehydrogenases are useful in converting biomass into biofuels and other industrial food products.Type: ApplicationFiled: January 4, 2011Publication date: July 7, 2011Applicants: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, Biotechnology Research And Development CorporationInventors: Huimin Zhao, Ryan Sullivan
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Patent number: 7972832Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.Type: GrantFiled: August 15, 2003Date of Patent: July 5, 2011Assignee: Danisco US Inc.Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
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Publication number: 20110159554Abstract: The invention provides: yeast having cellulose degradation ability that degrades cellulose outside the cell and glucose accumulation ability that accumulates, in a reaction liquid, glucose produced from cellulose as a result of the cellulose degradation ability, when the yeast was brought into contact with cellulose in the reaction liquid; and a method for producing glucose, wherein the glucose is produced from cellulose, the method comprising the step of bringing the yeast into contact with cellulose in a reaction liquid and the step of separating and collecting, from the reaction liquid, glucose accumulated in the reaction liquid as a result of the contact of the yeast with the cellulose.Type: ApplicationFiled: March 10, 2009Publication date: June 30, 2011Inventors: Katsuyuki Takahashi, Mitsufumi Wada, Sakurako Kimura, Ryota Fujii
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Publication number: 20110146138Abstract: The present invention relates to a method for producing fatty acid alkyl esters as well as cellulosic simplified sugars, shortened protein polymers, amino acids, or combination thereof resulting from the simultaneous esterification and hydrolysis, alcoholysis, or both of algae and other oil containing materials containing phospholipids, free fatty acids (FFA), glycerides, or combination thereof as well as polysaccharides, cellulose, hemicellulose, lignocellulose, protein polymers, or combination thereof in the presence of an alcohol and an optional acid catalyst.Type: ApplicationFiled: January 21, 2011Publication date: June 23, 2011Applicant: Inventure Chemical, Inc.Inventors: William W. Berry, Mark G. Tegen, William R. Sutterlin
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Publication number: 20110143402Abstract: The present invention describes a method for the treatment of lignocellulosic material which method comprises contacting said lignocellulosic material with a composition comprising two or more enzyme activities, said enzyme activities being cellulase and/or hemicellulase activities, wherein the pH during the treatment is about 4.5 or lower, and the treatment is carried out at a dry matter content of 15% or more.Type: ApplicationFiled: August 4, 2009Publication date: June 16, 2011Inventors: Wilhelmus Theodorus Antonius Maria De Laat, Manoj Kumar, Margot Elisabeth Francoise Schooneveld-Bergmans
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Patent number: 7960149Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: GrantFiled: February 17, 2010Date of Patent: June 14, 2011Assignee: Novozymes, Inc.Inventors: Paul Harris, Elizabeth Golightly
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Publication number: 20110129881Abstract: The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.Type: ApplicationFiled: November 24, 2010Publication date: June 2, 2011Inventors: Jie Yang, Xiyun Zhang, Attila Andor
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Patent number: 7951571Abstract: Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.Type: GrantFiled: October 28, 2008Date of Patent: May 31, 2011Assignee: Danisco US Inc.Inventors: Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Edmund Larenas
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Publication number: 20110124058Abstract: The present invention provides compositions and methods for the expression of recombinant ?-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.Type: ApplicationFiled: November 19, 2010Publication date: May 26, 2011Applicant: CODEXIS, INC.Inventors: Dipnath Baidyaroy, Louis Clark, Lisa M. Newman
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Publication number: 20110124056Abstract: Provided are methods for the pretreatment of ligno-cellulosic biomass such as softwoods with bisulfite such as ammonium bisulfite without the need for exogenous acid. In one variation, a method of pretreating ligno-cellulosic biomass is provided including the following steps: a) providing ligno-cellulosic biomass; b) contacting the ligno-cellulosic biomass with a solution comprising bisulfite at an amount between 1 and 10% of a dry weight of the ligno-cellulosic biomass to form a slurry; c) heating the slurry to a first temperature of 150-210° C. for a first period of time to form a first mixture; d) cooling the first mixture to a second temperature of 100-200° C. to form a second mixture; and e) maintaining the second mixture at the second temperature for a second period of time to form pretreated ligno-cellulosic biomass; wherein the first temperature is higher than the second temperature.Type: ApplicationFiled: September 29, 2010Publication date: May 26, 2011Applicant: CHEVRON U.S.A. INC.Inventors: Benjamin Levie, Rajesh Gupta, Johnway Gao, Dwight Anderson
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Publication number: 20110124057Abstract: An organic material production system using biomass material includes: a hydrothermal decomposition apparatus (13) that causes the biomass material (11) and hot compressed water (12) to countercurrently contact with each other and undergo hydrothermal decomposition, and that transfers a lignin component and a hemicellulose component into the hot compressed water, so as to separate the lignin component and the hemicellulose component from a biomass solid residue; a cellulose enzymatic saccharification device (17) that treats, with an enzyme, cellulose in the biomass solid residue, so as to enzymatically saccharify the cellulose to a first sugar solution containing hexose; an alcohol fermenter (18) that produces alcohols by fermentation using the obtained first sugar solution; a sulfuric acid decomposition device (33) that decomposes, with sulfuric acid, the hemicellulose component in hot water (30) discharged from the hydrothermal decomposition apparatus, which contains the eluted lignin component and the elutType: ApplicationFiled: October 2, 2008Publication date: May 26, 2011Applicant: MITSUBISHI HEAVY INDUSTRIES, LTD.Inventors: Minoru Genta, Ryosuke Uehara, Seiichi Terakura, Kinya Fujita
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Publication number: 20110099892Abstract: A transformed microorganism capable of converting an aldopentose to a ketopentose at a higher rate than the equivalent microorganism prior to transformation.Type: ApplicationFiled: July 3, 2009Publication date: May 5, 2011Applicant: TERRANOL A/SInventors: Ole Sibbesen, Birgitte Rönnow, Thomas Hvid Andersen
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Publication number: 20110091941Abstract: The invention relates to processes for hydrolyzing lignocellulose-containing material. The invention also relates to processes of producing fermentation products including a hydrolysis process of the invention.Type: ApplicationFiled: July 3, 2009Publication date: April 21, 2011Applicant: Novozymes A/SInventor: Hans Sejr Olsen
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Publication number: 20110091940Abstract: Disclosed are methods and systems for treating cellulose to make it more accessible for enzymatic or chemical modification. The invention includes treating cellulose with an alkali in an alcohol/water co-solvent system. The treatment decrystallizes or deaggregates the cellulosic material. The methods and systems increase the efficiency of enzymatic or chemical modifications of cellulose for use as biofuels or cellulose derivatives.Type: ApplicationFiled: April 3, 2009Publication date: April 21, 2011Applicant: CELLULOSE SCIENCES INTERNATIONAL, INC.Inventor: Rajai H. Atalla
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Patent number: 7927843Abstract: The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphates or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphates or the enzymes derived from the microorganism.Type: GrantFiled: July 25, 2007Date of Patent: April 19, 2011Assignees: Yuki Gosei Kogyo Co., Ltd., Novartis AGInventors: Sakayu Shimizu, Jun Ogawa
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Patent number: 7927842Abstract: The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphatase or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphatase or the enzymes derived from the microorganism.Type: GrantFiled: July 25, 2007Date of Patent: April 19, 2011Assignees: Yuki Gosei Kogyo Co. Ltd., Novartis AGInventors: Sakayu Shimizu, Jun Ogawa
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Publication number: 20110086386Abstract: A method of fractionating biomass, by permeability conditioning biomass suspended in a pH adjusted solution of at least one water-based polar solvent to form a conditioned biomass, intimately contacting the pH adjusted solution with at least one non-polar solvent, partitioning to obtain an non-polar solvent solution and a polar biomass solution, and recovering cell and cell derived products from the non-polar solvent solution and polar biomass solution. Products recovered from the above method. A method of operating a renewable and sustainable plant for growing and processing algae.Type: ApplicationFiled: December 8, 2010Publication date: April 14, 2011Inventors: Thomas J. Czartoski, Robert Perkins, Jorge L. Villanueva, Glenn Richards
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Publication number: 20110081689Abstract: Disclosed is a process for the thermal-mechanical pretreatment of biomass. The process includes subjecting a biomass feedstock including fibers containing cellulose, hemicellulose and lignin, to thermal reaction under conditions exceeding atmospheric pressure, at a temperature exceeding ambient temperature, at a predetermined moisture content and for a predetermined amount of time. Subsequently, the pressure of said thermal reaction is reduced under conditions resulting in explosive decompression of said biomass. The decompressed biomass is then subjected to axial shear forces to mechanically reduce the size of the fibers of the biomass to obtain treated biomass. The resultant treated biomass has a high level of enzymatic digestability and a low concentration of degradation products.Type: ApplicationFiled: October 6, 2010Publication date: April 7, 2011Applicant: KL ENERGY CORPORATIONInventors: Keith C. Flanegan, David B. Litzen, Dennis A. Harstad, James D. Schultze
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Patent number: 7919300Abstract: This invention describes a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt % or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt % fructose.Type: GrantFiled: July 28, 2004Date of Patent: April 5, 2011Inventors: Jun Wang, Rongzhao Fu, Dong Shen, Caike Jin, Zhangming Liu, Junming Chen
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Patent number: 7915017Abstract: A process for the treatment of biomass to render structural carbohydrates more accessible and/or digestible using concentrated ammonium hydroxide with or without anhydrous ammonia addition, is described. The process preferably uses steam to strip ammonia from the biomass for recycling. The process yields of monosaccharides from the structural carbohydrates are good, particularly as measured by the enzymatic hydrolysis of the structural carbohydrates. The monosaccharides are used as animal feeds and energy sources for ethanol production.Type: GrantFiled: September 17, 2007Date of Patent: March 29, 2011Assignee: Board of Trustees of MICHIGAN STATE UNIVERSITYInventor: Bruce Dale
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Publication number: 20110059492Abstract: The present invention relates to isolated polypeptides having alpha-amylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: May 14, 2009Publication date: March 10, 2011Applicant: Novozymes A/SInventors: Junxin Duan, Zheng Liu, Ming Li, Jim Liu, Guifang Wu
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Publication number: 20110045546Abstract: This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, ?-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.Type: ApplicationFiled: October 20, 2010Publication date: February 24, 2011Inventors: ALEXANDER V. GUSAKOV, TATYANA N. SALANOVICH, ALEXEY I. ANTONOV, BORIS B. USTINOV, OLEG N. OKUNEV, RICHARD P. BURLINGAME, MARK A. EMALFARB, MARCO A. BAEZ, ARKADY P. SINITSYN
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Publication number: 20110039311Abstract: The invention relates to processes process of producing fermentation products from lignocellulose-containing material comprising: a) pretreating the lignocellulose-containing material; b) preparing a slurry of pretreated lignocellulose-containing material and thermo treated distiller's grain; c) hydrolyzing the slurry with one or more cellulolytic enzymes; d) fermenting with a fermenting organism.Type: ApplicationFiled: March 27, 2009Publication date: February 17, 2011Applicant: Novozymes A/SInventors: Xin Li, Mads Peter Torry Smith
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Publication number: 20110039309Abstract: A process is described for producing fermentable sugars derivable from biomass that contains polysaccharide, such as cellulose, made increasingly accessible as a substrate for enzymatic degradation or other methods of depolymerization. These fermentable sugars are subsequently able to be fermented to produce various target chemicals, such as alcohols, aldehydes, ketones or acids.Type: ApplicationFiled: February 3, 2010Publication date: February 17, 2011Applicant: HERCULES INCORPORATEDInventors: Herbert T. CONNER, Patrick J. COWAN, John C. GAST
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Publication number: 20110039310Abstract: The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of the polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of the polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in the microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms.Type: ApplicationFiled: October 15, 2010Publication date: February 17, 2011Applicant: DSM IP ASSETS B.V.Inventors: Bastien CHEVREUX, Corina Hauk, Andrea Muffler, Nigel Mouncey, Masako Shinjoh
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Publication number: 20110033901Abstract: This invention is related, in part, to sulfatase enzymes and methods of their use.Type: ApplicationFiled: October 22, 2010Publication date: February 10, 2011Applicant: Massachusetts Institute of TechnologyInventors: James R. Myette, Ram Sasisekharan
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Publication number: 20110027839Abstract: An objective of the present invention is to provide a method for enzymatically saccharifying a cellulosic substance in an extremely efficient, simple, and inexpensive manner. A solution to the object is a mixture or a dried product thereof exhibiting cellulolytic activity containing a culture liquid or a supernatant liquid of a genus Trichoderma filamentous fungus and a culture product of Aspergillus kawachii.Type: ApplicationFiled: February 27, 2009Publication date: February 3, 2011Inventor: Kazuro Fukuda
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Publication number: 20110020881Abstract: In a method of producing biofuel using brown algae, Bacterium antarctica is used as a hydrolysis catalyst for saccharification to obtain monosaccharides from the brown algae. The saccharification with the hydrolysis catalyst is effective in saccharification of the brown algae.Type: ApplicationFiled: February 11, 2010Publication date: January 27, 2011Applicant: SAMSUNG ELECTRONICS CO., LTDInventors: Hwa Young CHO, Byung Jo YU, Jae Chan PARK, Sung Min PARK, Jae Hwa LEE
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Publication number: 20110016545Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: August 1, 2008Publication date: January 20, 2011Applicant: VERENIUM CORPORATIONInventors: Kevin Gray, Reinhard Dirmeier
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PROCESS FOR THE CONVERSION OF LIGNOCELLULOSIC SOLUTIONS CONTAINING A HIGH PROPORTION OF DRY MATERIAL
Publication number: 20110008850Abstract: The invention describes a process for the conversion of aqueous solutions of lignocellulosic solids comprising a solids content of between 1 and 20% of dry material, said process comprising a step a) for compression of said solution so as to separate the liquid phase present in and between the solids from the compressed solid phase and a step b) for extraction of at least the liquid phase, said liquid phase then being homogenised by heat and/or chemical treatments and reinjected on to the compressed solid phase.Type: ApplicationFiled: October 29, 2008Publication date: January 13, 2011Applicant: IFPInventor: Matthieu Rolland -
Publication number: 20110008849Abstract: The present invention relates to novel lactose phosphorylase enzymes and the uses thereof. More specifically, the invention relates to lactose phosphorylase enzymes created by mutation of a cellobiose phosphorylase from Cellulomonas uda. By introducing mutations in this enzyme, the activity can be switched from cellobiose phosphorylase into lactose phosphorylase.Type: ApplicationFiled: December 19, 2008Publication date: January 13, 2011Inventors: Tom Desmet, Manu De Groeve, Wim Soetaert
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Publication number: 20100291639Abstract: Cavitation is an often used mechanism for accelerating chemical reactions and for cleaning purposes. This invention describes a very simple process for generating cavitation with a vibration table through vertical excitations which generates a “water hammer” that generates very effective cavitation. A simple example is given for an industrial application of this process—splitting cellulose via sulfuric acid to glucose.Type: ApplicationFiled: May 9, 2010Publication date: November 18, 2010Inventor: Heiko Ackermann
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Publication number: 20100281582Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: April 22, 2010Publication date: November 4, 2010Applicant: Novozymes, Inc.Inventors: Kimberly Brown, Michelle Maranta, Eric Abbate
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Publication number: 20100278965Abstract: The invention concerns compositions and methods combining at least two phytases for hydrolyzing phytic acid (myo-inositol hexakis phosphate) into inorganic monophosphates, into myo-inositols with lower degree of phosphorylation and into free myo-inositol. Said compositions and methods are of particular interest for animal feeding.Type: ApplicationFiled: July 7, 2006Publication date: November 4, 2010Applicant: Adisseo France S.A.S.Inventors: Helene Boze, Guy Moulin
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Patent number: 7820414Abstract: Disclosed herein are Xylitol dehydrogenase-inactivated and arabinose reductase-inhibited mutant of Candida tropicalis, a method of producing a high yield of xylitol using the same, and xylitol produced by the method. More specifically, disclosed are a method for producing a high yield of xylitol, in which a high concentration of xylose contained in a biomass hydrolyzate is converted to xylitol using xylitol dehydrogenase-inactivated mutant of Candida tropicalis, without controlling dissolved oxygen to a low level, as well as xylitol produced according to the method. Also disclosed are a xylitol production method, in which the production of byproduct arabitol, which is produced when using a biomass as a substrate and adversely affects the yield of xylitol, is significantly reduced through the use of Candida tropicalis mutant ara-89 (KCTC 11136bp) having an inhibited activity of arabinose reductase converting arabinose to arabitol, thus increasing xylitol productivity, as well as xylitol produced by the method.Type: GrantFiled: June 29, 2007Date of Patent: October 26, 2010Assignees: LPBIO Co., Ltd., Korea Advanced Institute of Science and TechnologyInventors: Jung Hoe Kim, Byoung Sam Ko
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Patent number: 7816568Abstract: Catalytic reactions conducted during acid digestion of cellulose materials, including paper, a wide range of grasses including prairie grass, switch grass, pine wood sawdust, bagasse dried after sugar cane processing, cotton, waste cellulose products and starch materials, are taught for direct conversion to ethanol. The cellulose material is thoroughly wet in concentrated sulfuric acid in the presence of transition metal complexes possessing a degree of symmetry. Ethanol formed during the reaction can be removed by distillation affording a continuous process.Type: GrantFiled: December 17, 2007Date of Patent: October 19, 2010Inventor: Melvin Keith Carter
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Publication number: 20100261232Abstract: Novel variant ?-amylase enzymes are disclosed in which the residues corresponding to R179 and G180 in Bacillus stearothermophilus (SEQ ID NO.2) are deleted. The disclosed variant ?-amylase enzymes show altered or improved stability and/or activity profiles.Type: ApplicationFiled: March 9, 2009Publication date: October 14, 2010Applicant: GENENCOR INTERNATIONAL, INC.Inventors: Eugenio Ferrari, Marc Kolkman, Craig E. Pilgrim