Tryptophan; Tyrosine; Phenylalanine; 3,4 Dihydroxyphenylalanine Patents (Class 435/108)
  • Patent number: 10731187
    Abstract: The invention provides a recombinant microbial host cell capable of converting a raw material comprising a fermentable carbon substrate to o-aminobenzoate biologically. The invention further provides a method for producing aniline, comprising the steps of: a) producing o-aminobenzoate by fermentation of a raw material comprising at least one fermentable carbon substrate using the recombinant microbial host cell of the capable of converting said raw material comprising at least one fermentable carbon substrate to o-aminobenzoate biologically, wherein said o-aminobenzoate comprises anthranilate anion, b) converting said o-aminobenzoate from said anthranilate anion to anthranilic acid by acid protonation, c) recovering said anthranilic acid by precipitation or by dissolving in an organic solvent, and d) converting said anthranilic acid to aniline by thermal decarboxylation in an organic solvent.
    Type: Grant
    Filed: February 19, 2015
    Date of Patent: August 4, 2020
    Assignee: Covestro Deutschland AG
    Inventors: Gernot Jaeger, Jorgen Magnus, Amgad Salah Moussa, Guenter Olf, Giulio Lolli, Swantje Behnken, Jung-Won Youn, Mohammad Yalfani, Georg Sprenger
  • Patent number: 10612056
    Abstract: The present disclosure provides methods for preparing ?-substituted tryptophan compounds. The methods include: combining i) an unsubstituted indole or a substituted indole, ii) a ?-substituted serine, and iii) a tryptophan synthase ?-subunit (i.e., a TrpB); and maintaining the resulting mixture under conditions sufficient to form the ?-substituted tryptophan. The TrpB contains at least one amino acid mutation which promotes formation of an amino-acrylate intermediate. New TrpB variants and new ?-substituted tryptophan analogs are also described.
    Type: Grant
    Filed: May 3, 2018
    Date of Patent: April 7, 2020
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Christina E. Boville, Sabine Brinkmann-Chen, Andrew R. Buller, David K. Romney, Christopher K. Prier, Philipp Koch, Remkes A. Scheele
  • Patent number: 10570422
    Abstract: A method for the preparation of 2,4-dihydroxybutyric acid from homoserine includes a first step of conversion of the primary amino group of homoserine to a carbonyl group to obtain 2-oxo-4-hydroxybutyrate, and a second step of reduction of the obtained 2-oxo-4-hydroxybutyrate (OHB) to 2,4-dihydroxybutyrate.
    Type: Grant
    Filed: July 10, 2013
    Date of Patent: February 25, 2020
    Assignee: ADISSEO FRANCE S.A.S.
    Inventors: Thomas Walther, Hélène Cordier, Clémentine Dressaire, Jean Marie Francois, Robert Huet
  • Patent number: 10392672
    Abstract: The present invention relates to a strain of Rhodotorula sp. OMK-1(CCTCC M 2015326) and a method for producing natural cinnamic acid through the fermentation of Rhodotorula sp. OMK-1. The production method comprises steps of strain activation, seed culture, fermentation, extraction, etc. The fermentation process uses natural glucose, amino acids, etc. as raw materials to produce cinnamic acid via the metabolism of Rhodotorula sp. OMK-1. The present invention provides a safe and environmentally friendly method for producing natural cinnamic acid at low temperature and low pressure with simple operation and reduced pollution.
    Type: Grant
    Filed: January 13, 2016
    Date of Patent: August 27, 2019
    Assignee: XIAMEN OAMIC BIOTECHNOLOGY CO., LTD.
    Inventors: Chenguang Xing, Xijing Zhao, Zhiqiang Huang, Cuiping Chen, Wei Liu
  • Patent number: 10253307
    Abstract: Disclosed are a structure and a method capable of protecting from outside stimuli while containing in a liquid state a water-dispersible substance to be protected. The protective structure of the substance to be protected is a water-in-oil emulsion structure comprising an aqueous phase configuring a discontinuous phase containing the water-dispersible substance to be protected, an oil phase in which said aqueous phase is dispersed, and either vesicles formed with an amphiphilic substance which spontaneously forms vesicles or polycondensation polymer particles having hydroxyl groups.
    Type: Grant
    Filed: June 17, 2013
    Date of Patent: April 9, 2019
    Assignee: KANAGAWA UNIVERSITY
    Inventors: Kazuo Tajima, Yoko Imai
  • Patent number: 10174303
    Abstract: The invention provides genetically engineered microorganisms and methods for producing chorismate-derived products, such as para-hydroxybenzoic acid, salicylate, 2-aminobenzoate, 2,3-dihydroxybenzoate, and 4-hydroxycyclohexane carboxylic acid. Typically, the microorganism comprises at least one of (a) an exogenous chorismate pyruvate lyase, (b) an exogenous isochorismate synthase, (c) an exogenous isochorismate pyruvate lyase, and (d) a prephenate synthase comprising a disruptive mutation.
    Type: Grant
    Filed: May 26, 2016
    Date of Patent: January 8, 2019
    Assignee: LANZATECH NEW ZEALAND LIMITED
    Inventors: James Bruce Yarnton Haycock Behrendorff, Michael Koepke, Loan Phuong Tran, Wyatt Eric Allen
  • Patent number: 10167477
    Abstract: A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline.
    Type: Grant
    Filed: March 7, 2018
    Date of Patent: January 1, 2019
    Assignee: GENOMATICA, INC.
    Inventor: Priti Pharkya
  • Patent number: 10119155
    Abstract: Methods that may be used for the manufacture of the chemical compound (S)-norcoclaurine, (S)-norlaudanosoline, and (S)-norcoclaurine or [S]-norlaudanosoline synthesis intermediates are provided. (S)-Norcoclaurine, (S)-norlaudanosoline, and (S)-norcoclaurine or (S)-norlaudanosoline synthesis intermediates are useful as precursor products in the manufacture of certain medicinal agents.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: November 6, 2018
    Assignee: Epimeron Inc.
    Inventor: Peter James Facchini
  • Patent number: 10023886
    Abstract: The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c] from the corresponding ketones of the general formula [1][a] by using novel transaminases. These novel transaminases are selected from two different groups: either from a group of some 20 proteins with sequences as specified herein, or from a group of proteins having transaminase activity and isolated from a microorganism selected from the group of organisms consisting of Rahnella aquatilis, Ochrobactrum anthropi, Ochrobactrum tritici, Sinorhizobium morelense, Curtobacterium pusiffium, Paecilomyces lilacinus, Microbacterium ginsengisoli, Microbacterium trichothecenolyticum, Pseudomonas citronellolis, Yersinia kristensenii, Achromobacter spanius, Achromobacter insolitus, Mycobacterium fortuitum, Mycobacterium frederiksbergense, Mycobacterium sacrum, Mycobacterium fluoranthenivorans, Burkhoideria sp.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: July 17, 2018
    Assignee: Patheon Holdings I B.V.
    Inventors: Martin Schurmann, Wijnand Peter Helena Peeters, Natascha Hubertina Johannes Smeets, Helmut Schwab, Kerstin Steiner, Kateryna Mykolayivna Lypetska, Gernot Strohmeier
  • Patent number: 9732105
    Abstract: A compound having a structure of:
    Type: Grant
    Filed: November 13, 2015
    Date of Patent: August 15, 2017
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Peter Wipf, Marie-Celine Frantz
  • Patent number: 9701948
    Abstract: The present disclosure provides recombinant E. coli and other bacteria with reduced alcohol dehydrogenase and/or aldehyde reductase activity. The present disclosure further provides recombinant E. coli and other bacteria with reduced isobutyraldehyde reductase activity. Methods for the production and the uses of the recombinant bacteria are also provided. Specifically, recombinant bacteria further expressing 2-keto-acid decarboxylase can be used for producing higher aldehydes or other non-alcohol chemicals derived from aldehydes.
    Type: Grant
    Filed: June 18, 2013
    Date of Patent: July 11, 2017
    Assignee: The Regents Of The University Of California
    Inventors: Shota Atsumi, Gabriel Rodriguez
  • Patent number: 9587261
    Abstract: The present invention relates to microorganisms of Escherichia coli having enhanced L-tryptophan productivity and to a method for producing L-tryptophan using the same. More particularly, the present invention relates to an Escherichia coli variant in which repression and attenuation control of the tryptophan operon is released and accumulation of anthranilate is reduced and thereby enhancing L-tryptophan productivity. The present invention also relates to a method for producing L-tryptophan using the Escherichia coli variant.
    Type: Grant
    Filed: January 10, 2013
    Date of Patent: March 7, 2017
    Assignee: CJ Cheiljedang Corporation
    Inventors: Kwang Ho Lee, Hye Min Park, Hyo Hyoung Lee, Young Bin Hwang, Seok Myung Lee
  • Patent number: 9518280
    Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.
    Type: Grant
    Filed: October 28, 2014
    Date of Patent: December 13, 2016
    Assignee: CARGILL, INCORPORATED
    Inventors: Ellen Burke, Steven John Gort, Paula M. Hicks, Peter Luginbuhl, Sara C. McFarlan, Toby Richardson, Christopher Solheid, David P. Weiner, Lishan Zhao
  • Patent number: 9493777
    Abstract: The present invention provides a method for producing L-amino acids or salts thereof by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to overexpress the yajL gene.
    Type: Grant
    Filed: August 10, 2016
    Date of Patent: November 15, 2016
    Assignee: AJINOMOTO CO., INC.
    Inventors: Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Natalia Viktorovna Stoynova, Vera Georgievna Doroshenko
  • Patent number: 9441253
    Abstract: The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.
    Type: Grant
    Filed: February 7, 2014
    Date of Patent: September 13, 2016
    Assignee: William Marsh Rice University
    Inventors: Ka-Yiu San, George N. Bennett, Hui Wu
  • Patent number: 9428726
    Abstract: The present invention provides for a modified host cell comprising a heterologous expression of an efflux pump capable of transporting an organic molecule out of the host cell wherein the organic molecule at a sufficiently high concentration reduces the growth rate of or is lethal to the host cell.
    Type: Grant
    Filed: May 25, 2011
    Date of Patent: August 30, 2016
    Assignee: The Regents of the University of California
    Inventors: Mary J. Dunlop, Jay D. Keasling, Aindrila Mukhopadhyay
  • Patent number: 9180081
    Abstract: Improved formulations for transdermal delivery of botulinum toxin are disclosed. The formulations include, for example, botulinum toxin non-covalently associated with a positively charged backbone having branching or efficiency groups. The formulations also include a partitioning agent, oligo-bridge, or polyanion bridge, and may optionally contain a viscosity modifying agent. The formulations are designed for topical application onto the skin of a patient and may be used to treat wrinkles, hyperhidrosis, and other health-related problems. Kits for administration are also described.
    Type: Grant
    Filed: March 3, 2006
    Date of Patent: November 10, 2015
    Assignee: REVANCE THERAPEUTICS, INC.
    Inventors: Michael D. Dake, Jacob M. Waugh
  • Patent number: 9081199
    Abstract: The present invention provides: a protein having dipeptide-synthesizing activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant transformed with the recombinant DNA; a process for producing the protein having dipeptide-synthesizing activity using the transformant or the like; a process for producing a dipeptide using the protein having dipeptide-synthesizing activity; and a process for producing a dipeptide using, as an enzyme source, a culture of a transformant or a microorganism which produces the protein having dipeptide-synthesizing activity or the like.
    Type: Grant
    Filed: March 17, 2006
    Date of Patent: July 14, 2015
    Assignee: KYOWA HAKKO BIO CO., LTD.
    Inventors: Kuniki Kino, Yuji Nakazawa, Makoto Yagasaki
  • Publication number: 20150147788
    Abstract: The present invention relates to microorganisms of Escherichia coli having enhanced L-tryptophan productivity and to a method for producing L-tryptophan using the same. More particularly, the present invention relates to an Escherichia coli variant in which repression and attenuation control of the tryptophan operon is released and accumulation of anthranilate is reduced and thereby enhancing L-tryptophan productivity. The present invention also relates to a method for producing L-tryptophan using the Escherichia coli variant.
    Type: Application
    Filed: January 10, 2013
    Publication date: May 28, 2015
    Inventors: Kwang Ho Lee, Hye Min Park, Hyo Hyoung Lee, Young Bin Hwang, Seok Myung Lee
  • Publication number: 20150140614
    Abstract: The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
    Type: Application
    Filed: January 28, 2015
    Publication date: May 21, 2015
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: ALEXANDER RETH, BRIGITTE BATHE, STEPHAN HANS, WILFRIED CLAES
  • Patent number: 9034610
    Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.
    Type: Grant
    Filed: April 4, 2013
    Date of Patent: May 19, 2015
    Assignee: CARGILL, INCORPORATED
    Inventors: Timothy W. Abraham, Douglas C. Cameron, Paula M. Hicks, Sara C. McFarlan, James R. Millis, John Rosazza, David P. Weiner, Lishan Zhao
  • Patent number: 9005938
    Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.
    Type: Grant
    Filed: September 16, 2011
    Date of Patent: April 14, 2015
    Assignee: Cargill, Incorporated
    Inventors: Ellen Burke, Steven John Gort, Paula M. Hicks, Peter Luginbuhl, Sara C. McFarlan, Toby Richardson, Christopher Solheid, David Weiner, Lishan Zhao
  • Patent number: 8975046
    Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.
    Type: Grant
    Filed: November 2, 2011
    Date of Patent: March 10, 2015
    Assignee: Cargill, Incorporated
    Inventors: Brian J. Brazeau, Mervyn L. De Souza, Steven J. Gort, Paula M. Hicks, Sherry R. Kollmann, Jose M. Laplaza, Sara C. McFarlan, Fernando A. Sanchez-Riera, Christopher Solheid
  • Patent number: 8975045
    Abstract: A method for efficiently producing an L-amino acid utilizing a bacterium belonging to the family Enterobacteriaceae from a fatty acid or an alcohol such as glycerol as a raw material is provided. A bacterium belonging to the family Enterobacteriaceae which is able to produce L-amino acid and harbors an RpsA protein which has a mutation such that the native aspartic acid residue at position 210 is replaced with another amino acid residue is used. This bacterium is cultured in a medium containing a carbon source selected from a fatty acid and an alcohol, and the produced L-amino acid is collected from the medium.
    Type: Grant
    Filed: August 8, 2012
    Date of Patent: March 10, 2015
    Assignee: Ajinomoto Co., Inc.
    Inventors: Hidetaka Doi, Yusuke Hagiwara, Yoshihiro Usuda
  • Publication number: 20150056666
    Abstract: The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
    Type: Application
    Filed: November 4, 2014
    Publication date: February 26, 2015
    Applicant: Evonik Degussa GmbH
    Inventors: Alexander RETH, Brigitte Bathe, Stephan Hans, Wilfried Claes
  • Publication number: 20150056667
    Abstract: The present invention relates to a hydantoinase having an amino acid sequence selected from (i) or (ii), with (i) amino acid sequence selected from SEQ ID NO: 6-20 and SEQ ID NO: 73-119 (ii) amino acid sequence wherein in the amino acid sequence of SEQ ID NO: 6-20 and SEQ ID NO: 73-119, 1 to 75 amino acid residues have been substituted, deleted, inserted and/or added, and wherein further the catalytic activity of the hydantoinase is higher by a factor of at least 1.2 than the catalytic activity of the hydantoinase having amino acid sequence SEQ ID NO: 1, The present invention further relates to a process for preparing amino acids, wherein said hydantoinase is used.
    Type: Application
    Filed: November 16, 2012
    Publication date: February 26, 2015
    Inventors: Steffen Osswald, Heiko Schuster, Jürgen Roos, Andreas Karau, Ulrich Schwaneberg, Ronny Martinez, Hemanshu Mundhada, Ursula Holter
  • Publication number: 20150056665
    Abstract: A method of producing a chemical includes culturing cells in a culture solution in a fermentor to ferment a feedstock to produce a chemical; supplying the culture solution containing the chemical produced in the culturing to a plurality of separation membrane units arranged in parallel; filtering the culture solution supplied in the supplying to separate a permeate containing the chemical; refluxing a retentate that is not filtered in the filtering to the fermentor; and supplying a gas containing oxygen to the plurality of separation membrane units while a supply amount is changed to at least two different values to perform scrubbing, wherein the supply amount and supply time of the gas containing oxygen supplied in the culturing and the supplying the gas are set so that a kLa value is within a predetermined range from an optimal kLa value for the cells cultured in the culturing.
    Type: Application
    Filed: March 27, 2013
    Publication date: February 26, 2015
    Inventors: Satoko Kanamori, Hideki Sawai, Norihiro Takeuchi
  • Publication number: 20150050703
    Abstract: The present invention relates to a microorganism able to produce L-threonine or L-tryptophan, and to a method for producing L-threonine or L-tryptophan by using same. More specifically, the present invention relates to: recombinant Escherichia coli which is more efficient in producing L-threonine or L-tryptophan by increasing the ability to produce ATP which is used as the most plentiful energy source in cells when producing L-threonine or L-tryptophan; and a method for producing L-threonine or L-tryptophan by using same.
    Type: Application
    Filed: January 7, 2013
    Publication date: February 19, 2015
    Inventors: Ki Yong Cheong, Seok Myung Lee, Young Bin Hwang, Keun Cheol Lee, Kwang Ho Lee
  • Publication number: 20150044734
    Abstract: The present disclosure relates to engineered microorganisms that produce amino acids and amino acid intermediates. In particular, the disclosure relates to recombinant nucleic acids encoding operons that increase production of aromatic amino acids and the aromatic amino acid intermediate shikimate; microorganisms with increased production of aromatic amino acids and the aromatic amino acid intermediate shikimate; and methods related to the production of aromatic amino acids, the aromatic amino acid intermediate shikimate, and commodity chemicals derived therefrom.
    Type: Application
    Filed: August 31, 2012
    Publication date: February 12, 2015
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFONIA
    Inventors: Darmawi Juminaga, Jay D. Keasling
  • Publication number: 20150037849
    Abstract: Recombinant microbial cells and methods for producing 5-hydroxytryptophan (5HTP) using such cells are described. More specifically, the recombinant microbial cell comprises an exogenous gene encoding an L-tryptophan hydroxylase, and means for providing tetrahydrobiopterin (THB). Related sequences and vectors for use in preparing such recombinant microbial cells are also described.
    Type: Application
    Filed: February 28, 2013
    Publication date: February 5, 2015
    Inventors: Eric Michael Knight, Jiangfeng Zhu, Jochen Förster, Hao Luo
  • Patent number: 8945907
    Abstract: The present invention relates to a microorganism having L-tryptophan productivity and a method for producing L-tryptophan using the same. More precisely, the present invention relates to the recombinant E. coli strain CJ600 (KCCM 10812P) having tryptophan productivity produced from the mutant form (KFCC 10066) of E. coli having L-phenylalanine productivity, wherein tryptophan auxotrophy is released, L-phenylalanine biosynthesis is blocked but tryptophan productivity is enhanced by reinforcing the gene involved in tryptophan biosynthesis, and a method of producing L-tryptophan using the same.
    Type: Grant
    Filed: December 28, 2007
    Date of Patent: February 3, 2015
    Assignee: CJ Cheiljedang Corporation
    Inventors: Jae-yeong Ju, Hyang Choi, Eun-sung Koh, Ji-sun Lee, Jin-ho Lee, So-young Kim, Chang-hyun Jin, Young-hoon Park
  • Publication number: 20140377814
    Abstract: The present invention relates to a method for biologically treating carbon dioxide using the sulfur-oxidizing chemolithoautotroph Sulfurovum lithotrophicum 42BKT. The method of the present invention may enable carbon dioxide to be fixed or converted in high-concentration and high-pressure conditions which do not allow the biological photosynthetic conversion of microalgae or the like, and may exhibit high efficiency in the fixation of carbon dioxide as compared to existing methods for biologically treating carbon dioxide using microalgae. Further, the method of the present invention may use a gas mixture without a process of separating nitrogen and other gases, thus simplifying the process of the fixation or conversion of carbon diode.
    Type: Application
    Filed: September 20, 2012
    Publication date: December 25, 2014
    Inventors: Chang Ha Lee, Ik Sung Ahn, Hyuk Sung Kwon, Jae Hyuk Lee
  • Patent number: 8895270
    Abstract: Disclosed is a method for producing a ?-amino acid comprising a step of synthesizing a ?-amino acid from an ?-amino acid in the presence of an amino acid aminomutase. In this method, a ?-amino acid is precipitated as a solid in the reaction solution.
    Type: Grant
    Filed: September 30, 2008
    Date of Patent: November 25, 2014
    Assignee: Mitsui Chemicals, Inc.
    Inventor: Tomonori Hidesaki
  • Publication number: 20140315261
    Abstract: The present invention provides a method for producing L-amino acids by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the yjjK gene.
    Type: Application
    Filed: April 23, 2014
    Publication date: October 23, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Natalia Viktorovna Stoynova, Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Evgeniya Aleksandrovna Polyakova
  • Patent number: 8859243
    Abstract: The present invention provides a method for producing an L-amino acid by fermentation by culturing a microorganism having an L-amino acid-producing ability in a liquid medium to precipitate the L-amino acid, wherein a polymer such as a water-soluble cellulose derivative, a water-soluble polyvinyl compound, a polar organic solvent-soluble polyvinyl compound, a water-soluble starch derivative, an alginic acid salt, and a polyacrylic acid salt is added to the medium.
    Type: Grant
    Filed: April 7, 2009
    Date of Patent: October 14, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Satoshi Okutani, Shinya Fujiki, Shintaro Iwatani
  • Publication number: 20140287472
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Application
    Filed: June 11, 2014
    Publication date: September 25, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Patent number: 8835154
    Abstract: A microorganism of the genus Escherichia having enhanced L-amino acid productivity, wherein the microorganism is transformed to have an enhanced NAD kinase activity and an inactivated activity of an enzyme having an amino acid sequence of SEQ ID NO: 2 encoded by tehB gene and a method for producing L-amino acids using the microorganism of the genus Escherichia.
    Type: Grant
    Filed: January 18, 2012
    Date of Patent: September 16, 2014
    Assignee: CJ Cheiljedang Corporation
    Inventors: Kwang Ho Lee, Keun Chul Lee, Seok Myung Lee, Young Bin Hwang
  • Publication number: 20140227745
    Abstract: The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.
    Type: Application
    Filed: February 7, 2014
    Publication date: August 14, 2014
    Applicant: William Marsh Rice University
    Inventors: Ka-Yiu SAN, George N. BENNETT, Hui WU
  • Publication number: 20140212931
    Abstract: The present invention concerns iodoperoxidases from Zobellia galactanivorans, isolated nucleic acids encoding same, as well as methods for preparing these enzymes. Moreover, the invention is also directed to the use of such iodoperoxidases in a wide range of industrial, pharmaceutical, medical, cosmetics, and ecological applications, as well as in the food industry.
    Type: Application
    Filed: July 5, 2012
    Publication date: July 31, 2014
    Applicants: Universite Pierre et Marie Curie (Paris 6), Centre National de la Recherche Scientifique (CNRS)
    Inventors: Catherine Leblanc, Ludovic Delage, Gurvan Michel, Etienne Rebuffet, Mirjam Czjzek, Philippe Potin
  • Patent number: 8785162
    Abstract: The invention provides for aminotransferase and oxidoreductase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.
    Type: Grant
    Filed: December 31, 2008
    Date of Patent: July 22, 2014
    Assignee: Cargill, Incorporated
    Inventors: Mervyn L. De Souza, Paula M. Hicks, Sherry R. Kollmann, Jose Laplaza, Joshua M. Lundorff, Sara C. McFarlan, Erin Marasco, Wei Niu, Fernando A. Sanchez-Riera, Christopher Solheid, David P. Weiner, Peter Luginbuhl, Analia Bueno, Joslin Cuenca
  • Patent number: 8771997
    Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.
    Type: Grant
    Filed: October 12, 2011
    Date of Patent: July 8, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yasuaki Takakura, Hiroomi Ogino, Masakazu Sugiyama, Kenichi Mori, Eri Tabuchi, Koki Ishikawa, Uno Tagami, Hidemi Fujii
  • Publication number: 20140162325
    Abstract: The present invention provides a method for producing L-amino acid using a bacterium belonging to the family Enterobacteriaceae, particularly a motile bacterium belonging to the genus Escherichia, Enterobacter or Pantoea, wherein the bacterium has been modified so that expression of at least one gene of the flagella formation and motility cascade is enhanced.
    Type: Application
    Filed: February 11, 2014
    Publication date: June 12, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Irina Borisovna Altman, Tatyana Abramovna Yampolskaya, Leonid Romanovich Ptitsyn
  • Patent number: 8735132
    Abstract: The invention relates to identification of mutations and genetic targets for enhanced L-tyrosine production, and bacterial strains capable of L-tyrosine production.
    Type: Grant
    Filed: May 5, 2011
    Date of Patent: May 27, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Christine Santos, Gregory Stephanopoulos
  • Patent number: 8735107
    Abstract: This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. X?NO2, Cl, Br, NH2, OH, H, alkyl at one or several o, m, and p positions R?H or alkyl.
    Type: Grant
    Filed: November 2, 2011
    Date of Patent: May 27, 2014
    Assignee: Verenium Corporation
    Inventors: David P. Weiner, Alexander Varvak, Toby Richardson, Mircea Podar, Ellen Burke, Shaun Healey
  • Publication number: 20140141473
    Abstract: Yeast cell belonging to the genus Saccharomyces having introduced into its genome at least one xylA gene and at least one of each of araA, araB and araD genes and that is capable of consuming a mixed sugar mixture comprising glucose, xylose and arabinose, wherein the cell co-consumes glucose and arabinose, has genetic variations obtained during adaptive evolution and has a specific xylose consumption rate in the presence of glucose that is 0.25 g xylose/h, g DM or more.
    Type: Application
    Filed: April 20, 2012
    Publication date: May 22, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Panagiotis Sarantinopoulos, Wilbert Herman Marie Heijne, Aldo Greeve
  • Patent number: 8703446
    Abstract: The present invention provides a method for producing L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to enhance the expression of the bssR gene, which encodes a regulator of biofilm through signal secretion.
    Type: Grant
    Filed: January 7, 2013
    Date of Patent: April 22, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Mikhail Yurievich Kiryukhin, Mikhail Markovich Gusyatiner
  • Patent number: 8691537
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the rcsA gene.
    Type: Grant
    Filed: November 26, 2008
    Date of Patent: April 8, 2014
    Assignee: Ajinomoto Co., Ltd.
    Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • Publication number: 20140080185
    Abstract: A method is described for producing a target substance utilizing a microorganism by culturing the microorganism in a medium to produce and accumulate the target substance in the medium, and then collecting the target substance from culture. The microorganism is imparted with isomaltase activity, or modified to increase isomaltase activity.
    Type: Application
    Filed: November 20, 2013
    Publication date: March 20, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Ryo Takeshita, Naoki Kadotani, Kenji Abe
  • Publication number: 20140065679
    Abstract: The present application relates to a mutated Amycoiatopsis sp. TS-1-60 NAAAR that shows improved activity of the enzyme compared with the wild type Amycoiatopsis sp. TS-1-60 NAAAR. The mutated NAAAR is almost five times more active than its wild type counterpart. The present application also relates to the use of mutated Amycoiatopsis sp. TS-1-60 NAAAR in the production of enantiomerically pure amino acid from its N-acyl derivative via dynamic kinetic resolution method.
    Type: Application
    Filed: April 10, 2012
    Publication date: March 6, 2014
    Applicants: THE UNIVERSITY OF EDINBURGH, DR. REDDY'S LABORATORIES (EU) LIMITED
    Inventors: Scott Baxter, Dominic Campopiano, Karen Elizabeth Holt-Tiffin
  • Publication number: 20140024087
    Abstract: A microorganism of the genus Escherichia having enhanced L-amino acid productivity, wherein the microorganism is transformed to have an enhanced NAD kinase activity and an inactivated activity of an enzyme having an amino acid sequence of SEQ ID NO: 2 encoded by tehB gene and a method for producing L-amino acids using the microorganism of the genus Escherichia.
    Type: Application
    Filed: January 18, 2012
    Publication date: January 23, 2014
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: Kwang Ho Lee, Keun Chul Lee, Seok Myung Lee, Young Bin Hwang