Containing A Hetero Ring Of At Least Seven Ring Members (e.g., Zearalenone, Macrocyclic Lactones, Etc.) Patents (Class 435/124)
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Patent number: 10227558Abstract: The invention relates to a zinc-enriched biomass comprising living or subsequently killed micro-organisms, selected from the group consisting of Streptococcus thermophilus and Bifidobacterium animalis subsp. lactis and combinations thereof, a method for the manufacture of said zinc-enriched biomass, as well as food preparations, nutraceutic products, functional foods, cosmetic and cosmeceutic products, and food supplements, comprising the said biomass. Furthermore, new micro-organism strains are described which are able to concentrate zinc within the cell in very high amounts and therefore are particularly suitable for use in the method of the invention.Type: GrantFiled: October 27, 2016Date of Patent: March 12, 2019Assignee: BIOMAN S.R.L.Inventors: Alberto Benedetti, Francesco Girardo
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Publication number: 20140256960Abstract: A transformant is prepared to insert at least a gene expression cassette comprising a gene involved in the synthesis of 2-deoxy-scyllo-inosose into E. coli as host cells. A 2-deoxy-scyllo-inosose is synthesized from D-glucose, oligosaccharide, polysaccharide, starch and rice bran, using the transformant. A culture solution containing the 2-deoxy-scyllo-inosose is treated with a mixed bed or double bed type column comprising a hydrogen form of strong acidic cation exchange resin and an organic ion form of basic anion exchange resin. The 2-deoxy-scyllo-inosose as purified is reacted with trimethoxymethane to convert into 2-deoxy-scyllo-inosose dimethylketal, and the dimethylketal is crystallized and purified. Then, DOI is highly purified through hydrolyzing the dimethylketal in the presence of acid.Type: ApplicationFiled: February 12, 2014Publication date: September 11, 2014Applicants: MITSUI CHEMICALS, INC., NIIGATA BIO-RESEARCH PARK, INC.Inventors: Masamichi TAKAGI, Takahisa Kogure, Naoki Wakisaka, Hiroaki Takaku, Katsumi Ajisaka, Tatsuo Miyazaki, Masao Hirayama
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Publication number: 20140134672Abstract: Bioreactors, and particularly, photobioreactors having a reactor chamber and surge driver, and methods for using these devices, for example, for the production of carbon-based products are provided. The reactor chamber provides a housing for microorganisms and culture medium. The surge driver produces a surge of the microorganisms and/or culture medium in the reactor chamber.Type: ApplicationFiled: July 5, 2012Publication date: May 15, 2014Inventors: Max B. Tuttman, David A. St. Angelo
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Publication number: 20130244296Abstract: The present disclosure relates to an isolated or purified nucleotide sequence comprising a cDNA sequence of an rdc2 at least 60%, 70%, 90%, 95%, 98%, or 100% identical to SEQ ID NO. 1. In a second embodiment, the invention provides for a flavin-dependent halogenase comprising an amino acid sequence of an Rdc2 halogenase at least 60%, 70%, 90%, 95%, 98%, or 100% identical to SEQ ID NO. 2. In related embodiments there are provided herein methods for halogenating compounds by using an Rdc2 halogenase of the present invention.Type: ApplicationFiled: September 1, 2012Publication date: September 19, 2013Inventor: Jixun Zhan
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Publication number: 20130144029Abstract: The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.Type: ApplicationFiled: November 2, 2012Publication date: June 6, 2013Inventors: Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya, Mark J. Burk
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Patent number: 8420833Abstract: The present invention provides for a non-naturally occurring polyketide synthase (PKS) capable of synthesizing a carboxylic acid or a lactone, and a composition such that a carboxylic acid or lactone is included. The carboxylic acid or lactone, or derivative thereof, is useful as a biofuel. The present invention also provides for a recombinant nucleic acid or vector that encodes such a PKS, and host cells which also have such a recombinant nucleic acid or vector. The present invention also provides for a method of producing such carboxylic acids or lactones using such a PKS.Type: GrantFiled: April 29, 2009Date of Patent: April 16, 2013Assignee: The Regents of the University of CaliforniaInventors: Leonard Katz, Jeffrey L Fortman, Jay D Keasling
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Publication number: 20120198590Abstract: The disclosure pertains to isolated white pine toxigenic endophytes and the compounds produced by the fungal endophytes as well as methods and uses of the white pine endophytes. The isolated white pine toxigenic endophytes are useful for preparing white pine seedlings and plants that have increased tolerance to a pest and are prepared by inoculating a white pine seedling during the susceptible time window.Type: ApplicationFiled: January 27, 2012Publication date: August 2, 2012Applicant: J.D. IRVING, LIMITEDInventors: John David Miller, Greg William Adams, Mark Sumarah
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Patent number: 8164888Abstract: A computer having a mounting structure can be easily mounted to a wall. A bracket of the computer can receive a computer enclosure of the computer and easily mount a display and the computer enclosure of the computer to the wall.Type: GrantFiled: October 16, 2009Date of Patent: April 24, 2012Assignees: Hong Fu Jin Precision Industry (ShenZhen) Co., Ltd., Hon Hai Precision Industry Co., Ltd.Inventors: Xiao-Feng Ma, Lei Liu
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Publication number: 20120071549Abstract: The present invention relates to an anti-inflammatory use of macrolactin compounds such as macrolactin A, 7-O-malonyl macrolactin A and 7-O-succinyl macrolactin A, which are produced from a novel Bacillus strain of Bacillus polyfermenticus KJS-2 (KCCM10769P). The macrolactin compounds provided by the present invention were confirmed to greatly suppress the expression and formation of inducible nitric oxide synthetase (iNOS) and cyclooxygenase-2 (COX-2) which are proteins related to the formation of inflammatory mediators, and to accordingly inhibit the formation of nitric oxide (NO) and of prostaglandin E2 (PGE2) which are the metabolites of the proteins. In addition, the macrolactin compounds provided by the present invention were confirmed to have excellent effects in inhibiting the formation of tumor necrosis factor-alpha (TNF-?), interleukin-1? (IL-1?), interleukin-6 (IL-6) and granulocyte macrophage colony-stimulating factor (GM-CSF), which are pro-inflammatory cytokines.Type: ApplicationFiled: May 24, 2010Publication date: March 22, 2012Applicant: DAEWOO PHARMACEUTICAL IND. CO., LTD.Inventors: Young-Hoon Ji, Dong-Hee Kim, Jae-Seon Kang, Chun-Gyu Kim, Sung-Uk Chung, Sung-Woo Hwang, Kyung-Ran Kang
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Publication number: 20120052535Abstract: Methods for producing heterologous terpenes, terpenoids and/or small molecules in transgenic glandular trichome-bearing plants are provided, as well as the transgenic glandular trichome-bearing plants capable of producing the heterologous terpenes, terpenoids and small molecules. The genetically engineered glandular trichome-bearing plants contain and express one or more genes which encode proteins active in the biosynthetic pathways which produce the terpenes, terpenoids and small molecules. As a result, the essential oil of the transgenic plant is enriched for the heterologous or homologous terpenes, terpenoids and/or small molecules Storage of the essential oil in the glandular trichomes of the plant reduces the volatility and cytotoxic capacity of the heterologous molecules, thereby increasing yield and decreasing damage to the transgenic plant.Type: ApplicationFiled: March 26, 2010Publication date: March 1, 2012Inventors: Bernd Markus Lange, Rigoberto Rios-Estepa, Glenn W. Turner
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Publication number: 20110287471Abstract: An isolated fungus is described. The isolated fungus produces at least one compound selected from the group consisting of 1,8-cineole, 1-methyl-1,4-cyclohexadiene, and (+)-?-methylene-?-fenchocamphorone. A method for producing at least one compound selected from the group consisting of 1,8-cineole, 1-methyl-1,4-cyclohexadiene, and (+)-?-methylene-?-fenchocamphorone is also described. The method includes culturing a fungus on or within a culturing media in a container under conditions sufficient for producing the at least one compound.Type: ApplicationFiled: May 18, 2011Publication date: November 24, 2011Inventors: Gary A. Strobel, Angela R. Tomsheck
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Publication number: 20110171696Abstract: Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.Type: ApplicationFiled: March 18, 2011Publication date: July 14, 2011Applicant: Celexion, LLCInventors: Brian M. Baynes, John Michael Geremia
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Publication number: 20110034528Abstract: The present invention describes Elansolids, a new class of novel anti-bacterial compounds of formula (I).Type: ApplicationFiled: February 19, 2009Publication date: February 10, 2011Inventors: Klaus Gerth, Heinrich Steinmetz, Gerhard Höfle
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Publication number: 20110020867Abstract: Improved constructs for increasing efficiency of transformation of thermophilic host cells for production of carbon-based products of interest and methods for producing carbon-based products of interest are provided.Type: ApplicationFiled: July 20, 2010Publication date: January 27, 2011Applicant: JOULE UNLIMITED, INC.Inventors: Nikos B. Reppas, Brian D. Green
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Publication number: 20110021790Abstract: The present invention provides for a non-naturally occurring polyketide synthase (PKS) capable of synthesizing a carboxylic acid or a lactone, and a composition such that a carboxylic acid or lactone is included. The carboxylic acid or lactone, or derivative thereof, is useful as a biofuel. The present invention also provides for a recombinant nucleic acid or vector that encodes such a PKS, and host cells which also have such a recombinant nucleic acid or vector. The present invention also provides for a method of producing such carboxylic acids or lactones using such a PKS.Type: ApplicationFiled: April 29, 2009Publication date: January 27, 2011Applicant: The Regents of the University of CaliforniaInventors: Leonard Katz, Jeffrey L. Fortman, Jay D. Keasling
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Publication number: 20100121079Abstract: Disclosed are: a novel dinoflagellate alga; and a macrolide compound which can be used as a novel anticancer agent. Specifically disclosed is a macrolide compound produced by a dinoflagellate Amphidinium sp. Strain HYA002 or HYA0024. The macrolide compound has excellent proliferation-inhibiting effect against a human tumor cell and therefore can be used as a novel anticancer agent.Type: ApplicationFiled: December 19, 2007Publication date: May 13, 2010Inventors: Masashi Tsuda, Keiko Oguchi, Rie Iwamoto, Yumiko Okamoto
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Patent number: 7667052Abstract: The present invention provides a novel bioactive substance having an antitumor activity and a process for producing it, and a medical use thereof. Namely, it provides a 12-membered ring macrolide compound represented by the following formula obtained from the incubation solution of Streptomyces sp. Mer. 11107 or a variant thereof, a pharmacologically acceptable salt thereof or a hydrate of them, and a process for producing it.Type: GrantFiled: August 30, 2005Date of Patent: February 23, 2010Assignee: Eisai R&D Management Co., Ltd.Inventors: Yoshiharu Mizui, Takashi Sakai, Satoshi Yamamoto, Keisuke Komeda, Masanori Fujita, Akifumi Okuda, Kumiko Kishi, Jun Niijima, Mitsuo Nagai, Kiyoshi Okamoto, Masao Iwata, Yoshihiko Kotake, Toshimitsu Uenaka, Naoki Asai, Motoko Matsufuji, Tomohiro Sameshima, Naoto Kawamura, Kazuyuki Dobashi, Takashi Nakashima, Masashi Yoshida, Toshio Tsuchida, Susumu Takeda, Tomonari Yamada, Koji Norihisa, Takao Yamori
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Publication number: 20100009418Abstract: A method of increasing cellular NADPH levels by expressing one or more genes that encode an enzyme that causes the production of NADPH. The system is combined with other enzymes that require NADPH, thus improving the overall yield of the desired product.Type: ApplicationFiled: August 29, 2007Publication date: January 14, 2010Applicant: Rice UniversityInventors: Ka-Yiu San, George Bennett, Henry Lin, Irene Martinez, Jiangfeng Zhu
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Publication number: 20090286291Abstract: The present invention relates to the biosynthesis of polyketides and derives from the cloning of nucleic acids encoding a polyketide synthase and other associated proteins involved in the synthesis of the polyketide borrelidin. Materials and Methods including enzyme systems, nucleic acids, vectors and cells are provided for the preparation of polyketides including borrelidin and analogues and derivatives thereof. Novel polyketide molecules are also provided.Type: ApplicationFiled: June 9, 2009Publication date: November 19, 2009Inventors: Jose A. Salas, Carmen Mendez, Carlos Olano, Cesar Sanchez, Alfredo F. Brana, Barrie Wilkinson, Christine J. Martin, Steven Moss, Peter F. Leadlay, Marko Oliynyk
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Publication number: 20090075327Abstract: The invention relates to the production of secondary metabolites by fungi. More particularly, the invention relates to improvement of production of commercially important secondary metabolites by fungi. The invention provides methods for improving secondary metabolite production in a fungus, comprising modulating the expression of a gene involved in regulation of secondary metabolite production.Type: ApplicationFiled: March 7, 2001Publication date: March 19, 2009Inventors: Robert Busby, Brian Cali, Peter Hecht, Doug Holtzman, Kevin Madden, Mary Maxon, Todd Milne, Thea Norman, John Royer, Sofie Salama, Amir Sherman, Jeff Silva, Eric Summers
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Patent number: 7396667Abstract: A depolymerizing process of polylactic acid, wherein the polylactic acid is depolymerized in the presence of a hydrolase in an organic solvent or a supercritical fluid, thereby producing a re-polymerizable oligomer. A producing process of polylactic acid, wherein the re-polymerizable oligomer obtained by the above-mentioned depolymerization process is polymerized in the presence of a hydrolase or a polymerization catalyst.Type: GrantFiled: July 30, 2003Date of Patent: July 8, 2008Assignee: Keio UniversityInventor: Shuichi Matsumura
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Publication number: 20080125326Abstract: The present invention relates to ?17 desaturases, which have the ability to convert ?-6 fatty acids into their ?-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these ?17 desaturases in oleaginous yeast are disclosed.Type: ApplicationFiled: July 19, 2007Publication date: May 29, 2008Inventors: Narendra S. Yadav, Zhixiong Xue, Quinn Qun Zhu
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Publication number: 20080070286Abstract: The present invention provides a DNA participating in biological transformation of a macrolide compound 11107B. The present invention provides, particularly, a DNA participating in biological transformation of a macrolide compound 11107B represented by the formula (I) into a 16-position hydroxy macrolide compound 11107D represented by the formula (II), the DNA encoding a protein having 16-position hydroxylating enzymatic activity or ferredoxin, to a method of isolating the DNA, to a protein encoded by the DNA, a plasmid carrying the DNA, a transformant obtained by transforming using the plasmid and a method of producing a 16-position hydroxy compound by using the transformant.Type: ApplicationFiled: November 25, 2004Publication date: March 20, 2008Inventors: Kazuhiro Machida, Yasuhide Aritoku, Toshio Tsuchida, Takashi Nakashima
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Patent number: 7125692Abstract: A process for producing a monomer for resists represented by the following general formula 1: wherein R1 represents hydrogen or an optionally substituted alkyl group; R2, R3 and R4 each independently represent hydrogen or a substituent; and X, Y and Z each independently represent a direct bond or an optionally substituted alkylene group with 1 to 3 chain members, the process comprising carrying out esterification or transesterification in the presence of a biocatalyst.Type: GrantFiled: September 11, 2002Date of Patent: October 24, 2006Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Eiji Satou, Makoto Kaneko, Naoshi Murata
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Patent number: 7052885Abstract: A process for preparing enantiomer-enriched heterocyclic (R)- and (S)-cyanohydrins of the formula (I), where R1, R2, R3, R4 independently of one another are H, an unsubstituted or substituted C1–C24-alkyl, alkenyl or alkynyl radical, where one or more carbon atoms in the chain can be replaced by an oxygen atom, a nitrogen atom, a sulfur atom, an SO or an SO2 group, an unsubstituted or substituted aryl or heteroaryl radical or heterocyclic radical or halogen, hydroxyl, NR5R6, acetyl, oxo, C1–C6-carbalkoxy, C1–C6-carbalkoxyamino, COOR7, cyano, amide, benzoylamino or NO2, R5 and R6 are H, C1–C6-alkyl radical, phenyl radical, benzyl radical or COOR7, or together form a C2–C8-alkylene or heteroalkylene radical, R7 is H or C1–C6-alkyl, n is 0, 1, 2 or 3, X, Y and Z can be an unsubstituted or substituted carbon atom or a radical selected from the group consisting of N, O, S or NR5R6, where R5 and R6 are as defined above, SO or SO2, and at least one of the radicals X, Y and Z is not a carbon atom, where the compoType: GrantFiled: December 23, 2002Date of Patent: May 30, 2006Assignee: DSM Fine Chemicals Austria NFG GmbH & Co KGInventors: Peter Poechlauer, Wolfgang Skranc, Herbert Mayrhofer, Irma Wirth, Rudolf Neuhofer, Herfried Griengl, Martin Fechter
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Patent number: 6680055Abstract: The present invention provides, inter alia, polyketide macrolides including a polyketide macrolide of the structure wherein R1-R5 are the same or different and each is independently selected from the group consisting of hydrogen, R6, a C(O)R7, a C(S)R7, a C(O)NHR7, and a C(S)NHR7, each occurrence of R6 is independently selected from the group consisting of a C1-C6 alkyl, a C5-C12 aryl, and a sugar, each occurrence of R7 is independently selected from the group consisting of hydrogen, a C1-C6 alkyl, and a C5-C12 aryl, and wherein R1 and R2, R2 and R3, and/or R4 and R5 can be taken together to form a ketal ring. Also provided are asceptic mixtures of polyketide macrolides isolated from M.Type: GrantFiled: February 27, 2002Date of Patent: January 20, 2004Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Pamela L. C. Small, Kathleen M. George
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Publication number: 20030175678Abstract: The invention features a method for identifying a gene associated with a desired phenotype. This method includes the steps of: (a) providing a plurality of cell cultures that include plant, animal, or fungal cells capable of exhibiting a desired phenotype; (b) contacting each of at least a subset of said cells with a stimulus that (i) induces said cells to exhibit the phenotype, or (ii) does not induce said cell cultures to exhibit the phenotype; (c) determining the presence of the phenotype in the cell cultures of step (b); and (d) identifying a gene having increased expression in response to stimuli that induce the phenotype but do not have increased expression in response to stimuli that do not induce the phenotype.Type: ApplicationFiled: January 24, 2002Publication date: September 18, 2003Inventors: Benjamin A. Bowen, Edward A. Deakin, Neil Goldsmith, Christian Haudenschild, David R. Houck, James B. McAlpine, Soren V.S. Nielsen, Christopher Pazoles, Margaret E. Spencer, Angela M. Stafford
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Publication number: 20030092140Abstract: Facile methods for preparing diketide and triketide thioesters are disclosed. The resulting thioesters may be used as intermediates in the synthesis of desired polyketides, and may contain functional groups which ultimately reside in side chains on the resulting polyketide and thus can be used further to manipulate the polyketide so as form derivatives. The polyketides produced may also be tailored by glycosylation, hydroxylation and the like. New polyketides and their derivatives and tailored forms are thereby produced.Type: ApplicationFiled: August 8, 2002Publication date: May 15, 2003Inventors: Gary Ashley, Isaac C. Chan-Kai, Mark A. Burlingame
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Publication number: 20020168733Abstract: A method for microbial production of epoxides and other oxygenated products is disclosed. The method uses a biocatalyst of methanotrophic bacteria cultured in a biphasic medium containing a major amount of a non-aqueous polar solvent. Regeneration of reducing equivalents is carried out by using endogenous hydrogenase activity together with supplied hydrogen gas. This method is especially effective with gaseous substrates and cofactors that result in liquid products.Type: ApplicationFiled: March 12, 2001Publication date: November 14, 2002Applicant: Bechtel BWTX Idaho, LLCInventors: Thomas R. Clark, Francisco F. Roberto
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Patent number: 6479657Abstract: A process for producing crystal 1-kestose wherein granular crystal 1-kestose in the form of large crystals can be produced at a high yield is disclosed. A highly pure solution of 1-kestose is concentrated to a Brix of 75 or higher; either seed crystals are added, or the solution is vacuum-concentrated to generate microcrystals for use as nuclei; then, a crystal growing step by vacuum-concentration and a microcrystal dissolving step for redissolving microcrystals which have formed in the concentrate are repeated at least twice each. Alternatively, a highly pure solution of 1-kestose is concentrated to a Brix of 80 or higher; either seed crystals are added, or the solution is allowed to initiate crystallization; after crystals are allowed to grow, a cooling step where the temperature is lowered by 5° C. to 20° C. from the previous step and a crystal growing step where the concentrate is maintained at the temperature to allow the crystals to grow are repeated at least twice each.Type: GrantFiled: September 29, 1998Date of Patent: November 12, 2002Assignee: Meiji Seika Kaisha, Ltd.Inventors: Koji Nishizawa, Hitoshi Matsumoto, Hirofumi Nakamura, Takashi Kawakami, Yuko Nakada, Masao Hirayama, Takashi Adachi
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Publication number: 20020128250Abstract: The invention relates to the production of secondary metabolites by fungi. More particularly, the invention relates to improvement of production of commercially important secondary metabolites by fungi. The invention provides methods for improving secondary metabolite production in a fungus, comprising modulating the expression of a gene involved in regulation of secondary metabolite production.Type: ApplicationFiled: March 7, 2001Publication date: September 12, 2002Inventors: Robert Busby, Brian Cali, Peter Hecht, Doug Holtzman, Kevin Madden, Mary Maxon, Todd Milne, Thea Norman, John Royer, Sofie Salama, Amir Sherman, Jeff Silva, Eric Summers
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Publication number: 20010024811Abstract: Modified PKS gene clusters which produce novel polyketides in an efficient system in a host cell or in a cell free extract are described.Type: ApplicationFiled: May 29, 2001Publication date: September 27, 2001Inventors: Chaitan Khosla, Rembert Pieper, Guanglin Luo, David E. Crane, Camilla Kao
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Patent number: 6284512Abstract: The present invention relates to a method for screening chemically modified mutant enzymes for amidase and/or esterase activity. This method includes providing a chemically modified mutant enzyme with one or more amino acid residues from an enzyme being replaced by cysteine residues, where at least some of the cysteine residues are modified by replacing thiol hydrogen in the cysteine residues with a thiol side chain, contacting the chemically modified mutant enzyme with a substrate for an amidase and/or a substrate for an esterase, and determining whether the chemically modified mutant enzyme exhibits amidase and/or esterase activity. The present invention also relates to chemically modified mutant enzymes and a method of producing them where one or more amino acid residues from an enzyme are replaced by cysteine residues, and the cysteine residues are modified by replacing at least some of the thiol hydrogen in the cysteine residue with a thiol side chain to form the chemically modified mutant enzyme.Type: GrantFiled: January 21, 1999Date of Patent: September 4, 2001Assignees: Genencor International, Inc.Inventors: J. Bryan Jones, Erika Plettner
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Patent number: 5965604Abstract: The present invention relates to chrolactomycin compound represented by the following formula (I): ##STR1## or pharmaceutically acceptable salts thereof having antibacterial and antitumor activities.Type: GrantFiled: December 17, 1998Date of Patent: October 12, 1999Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Yoshinori Yamashita, Ryuichiro Nakai, Tamio Mizukami, Shingo Kakita, Shigeru Chiba, Shiro Akinaga
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Patent number: 5900366Abstract: A process for the fed batch production of a sophorolipid composition is described, in which culturing takes place of at least one Candida bombicola strain in a culture medium incorporating a sugar and a nitrogen source and said cultured strain is exposed in a reaction zone to a supply of an appropriate substrate under adequate aeration, temperature and pH conditions and at least once the following sequence is performed: a) the strain is continuously supplied with substrate at a supply rate in the reaction zone between 0.Type: GrantFiled: June 18, 1993Date of Patent: May 4, 1999Assignee: Institut Fran.cedilla.ais Du PetroleInventors: Remy Marchal, Jeannine Lemal, Caroline Sulzer, Anne-Marie Davila
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Patent number: 5817489Abstract: Compositions comprising novel di-nitrogen heterocycle compounds containing at least one N-meta-substituted alkaryl group are prepared. The compounds of the present invention are useful as antibacterial and as other pharmaceutical agents and as intermediates for preparation of other pharmaceutical agents. In addition, compounds of the present invention are useful as research reagents.Type: GrantFiled: August 1, 1996Date of Patent: October 6, 1998Assignee: ISIS Pharmaceuticals, Inc.Inventors: Phillip Dan Cook, Pei Pei Kung, Andrew M. Kawasaki
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Patent number: 5763233Abstract: This invention provides a process for the production of .delta.-decalactone by the microbial reduction of massoia lactone, characterized in that a bacterium having the ability to reduce massoia lactone is used as the microorganism. The .delta.-decalactone produced according to this process has a highly tastable, mild creamlike scent and flavor, and is hence suitable for use in flavor compositions.Type: GrantFiled: July 31, 1997Date of Patent: June 9, 1998Assignee: T. Hasegawa Co., Ltd.Inventors: Shinobu Gocho, Kitazawa Rumi, Komai Tsuyoshi
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Patent number: 5753471Abstract: A process for the biotechnological preparation of alcohols, aldehydes and carboxylic acids by oxidation of compounds of the formula R.sup.1 --C(CH.sub.3).dbd.CR.sup.2 R.sup.3, where R.sup.1, R.sup.2 and R.sup.3 have the meanings indicated in the description, with the aid of microorganisms is described.Type: GrantFiled: December 28, 1995Date of Patent: May 19, 1998Assignee: BASF AktiengesellschaftInventors: Uwe Pressler, Friedhelm Balkenhohl, Bernhard Hauer, Wolfgang Ladner, Ursula Schnell, Horst Ralf Staudenmaier
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Patent number: 5646022Abstract: Gamma lactones with 9 carbon atoms, saturated or unsaturated and mixtures thereof, in natural form, are obtained by:A) culturing a microorganism selected from the group comprising Pichia ohmeri and Pichia stipitis or both of these in a substrate comprising an unsaturated C.sub.18 hydroxy-acid with the hydroxyl in position C.sub.13 to obtain a mixture comprising gamma nonanolide;or, alternatively,B) subjecting to lipoxygenation a substrate comprising an acid selected from the group comprising linoleic acid and linolenic acid or mixtures thereof anddistilling the product of lipoxygenation in a current of steam to obtain a mixture comprising an unsaturated gamma lactone that is gamma-2-nonenolide, gamma-2,6-nonadienolide or mixtures thereof.Type: GrantFiled: July 28, 1995Date of Patent: July 8, 1997Inventors: Mario Villa, Claudio Fuganti, Gioia Zucchi, Gianna Allegrone, Masimo Barbeni, Paolo Cabella
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Patent number: 5641804Abstract: The invention is directed to compounds having general formula (I) or general formula (II). Compounds of formulae (I) or (II) have interesting antifungal activities and may, accordingly, be used as active ingredients in fungicidal compositions.Type: GrantFiled: March 3, 1995Date of Patent: June 24, 1997Assignee: Novo Nordisk A/SInventors: Jens Breinholt, Ruby Ione Nielsen
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Patent number: 5561060Abstract: Penicillium cultures capable of producing 10-member ring lactones.It is possible with the aid of Penicillium strains to prepare new 10-membered ring lactones having a pharmacological action.Type: GrantFiled: April 12, 1995Date of Patent: October 1, 1996Assignee: Hoechst AktiengesellschaftInventors: Joachim Wink, Susanne Grabley, Gerhard Seibert, Klaus Hutter, Axel Zeeck
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Patent number: 5413960Abstract: The antibiotic .beta.-hydroxypropionaldehyde is obtained by cultivating strains of Lactobacillus reuteri under anaerobic conditions in the presence of glycerol or glyceraldehyde. This antibiotic has activity against Gram positive and Gram negative bacteria, against the yeast, Saccharomyces cerevisiae, and against the protozoan, Trypanosoma cruzi, and decreases ribonucleotide reductase activity.Type: GrantFiled: July 27, 1993Date of Patent: May 9, 1995Assignee: Biogaia ABInventors: Walter J. Dobrogosz, Sven E. Lindgren
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Patent number: 5352586Abstract: The antibiotic reuterin is obtained by cultivating strains of Lactobacillus reuteri under controlled conditions. Reuterin has inhibitory activity against Gram positive and Gram negative bacteria, against yeast, Saccharomyces cerevisiae, and against the protozoan, Trypanosoma cruzi. Reuterin producing strains are identified by grown inhibition of susceptible microorganisms in the presence of glycerol or glyceraldehyde.Type: GrantFiled: March 18, 1991Date of Patent: October 4, 1994Assignee: Biogaia ABInventors: Walter J. Dobrogosz, Sven E. Lindgren
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Patent number: 5281533Abstract: Scytophycins A, B, C, D and E, an aldehyde and alcohol derivative of scytophycin B and the specified alkanoyl and benzoyl esters of scytophycins A, B, C, D and E and the B derivatives (scytophycin compounds) are novel antineoplastic and antifungal agents. Antifungal compositions containing a scytophycin compound and methods of inhibiting fungi, especially plant pathogenic fungi, using these compositions are included. A method for producing the scytophycin complex, comprising scytophycins A, B, C, D and E, using a new strain of the blue-green alga Scytonema pseudohofmanni, and a biologically purified culture of the alga are also provided.Type: GrantFiled: November 19, 1990Date of Patent: January 25, 1994Assignee: University of HawaiiInventors: Richard E. Moore, Eiichi Furusawa, Ted R. Norton, Gregory M. L. Patterson, Jon S. Mynderse
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Patent number: 5278068Abstract: An organism of the Cytospora species produces substituted hexahydrobenzopyran compounds of the Formulas A, B, or C which are useful as angiotensin II antagonists: ##STR1##Type: GrantFiled: October 16, 1992Date of Patent: January 11, 1994Assignee: Merck & Co., Inc.Inventor: Gerald F. Bills
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Patent number: 5266484Abstract: Disclosed is a method for selecting a mutant strain belonging to the genus Streptomyces which is a hyper-producer of Streptovaricin C superior to those know heretofore. This is accomplished by first subjecting a natural strain of Streptomyces spectabilis to conditions so as to isolate organisms which are streptovaricin resistant. The streptovaricin resistant organisms thus isolated are then subjected to mutagenesis and then cultured. The colonies which are asporogenous are individually cultured in fermentation batches such that the strains take the form of pellets of varying sizes and colors. From the batch having the most heterogeneous mixture of pellets, the smallest pellet or the pellet(s) having the deepest color (usually deep red or crimson) is isolated. We have discovered that the strain of this pellet has a high likelihood of being a hyperproducer of streptovaricin. The strain of this pellet may then be subjected to fermentation conditions to produce streptovaricin.Type: GrantFiled: April 23, 1992Date of Patent: November 30, 1993Assignees: Shin-Etsu Bio, Inc., Shin-Etsu Chemical Co., Ltd.Inventors: Kaname Inoue, Motohide Yamazaki, Kanji Murofushi, Richard W. Armentrout
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Patent number: 5258291Abstract: A method for separation and recovery of polymeric beads from an antibiotic fermentation broth comprising suspending a mixture of the beads and any inherent mold from the fermentation in an aqueous solution having a specific gravity which is effective to cause the beads to form a discrete layer at or on the surface of the solution, separate and apart from the mold. The separate layer of the beads may then be easily removed from the liquid by a conventional physical methods.Type: GrantFiled: February 12, 1993Date of Patent: November 2, 1993Assignees: Shin-Etsu Bio. Inc., Shin-Etsu Chemical Co., Ltd.Inventors: Kaname Inoue, Motohide Yamazaki, Richard W. Armentrout
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Patent number: 5242815Abstract: A method for producing streptovaricin by culturing a streptovaricin producing strain in the presence of a nonionic adsorbent. This procedure substantially increases the production efficiency of the streptovaricin. A second embodiment wherein the streptovaricin producing strain is produced in the presence of fumaric acid or its water soluble salts to provide improvements in production efficiency is also disclosed.Type: GrantFiled: April 29, 1992Date of Patent: September 7, 1993Assignees: Shin-Etsu Chemical Co., Ltd., The Institute of Physical Chemical ResearchInventors: Isao Endo, Shigehiro Nagura, Kaname Inoue, Jun Watanabe
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Patent number: 5236832Abstract: A microbiological process for the oxidation of methyl groups in aromatic 5- or 6-member ring heterocycles to the corresponding carboxylic acid. The heterocycle serves as the substrate and exhibits no substituent on the carbon atoms adjacent to the methyl groups to be oxidized. The reaction of the heterocycle takes place by means of microorganisms of the genus Pseudomonas utilizing toluene, xylene, or cymene. The enzymes of the microorganisms been previously induced.Type: GrantFiled: October 30, 1991Date of Patent: August 17, 1993Assignee: Lonza, Ltd.Inventor: Andreas Kiener
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Patent number: 5213973Abstract: A microbiological process for oxidation of methyl groups in aromatic 5- or 6-ring heterocycles to the corresponding carboxylic acid. The reaction of the heterocycle takes place by microorganisms of the genus Pseudomonas, which utilize toluene, xylene or cymene. An inducer, the aromatic heterocycle, as the substrate, and a carbon source and energy source are fed in and, after the maximal product concentration is reached, the product is separated.Type: GrantFiled: July 3, 1991Date of Patent: May 25, 1993Assignee: Lonza Ltd.Inventor: Frans Hoeks