Containing A Hetero Ring Of At Least Seven Ring Members (e.g., Zearalenone, Macrocyclic Lactones, Etc.) Patents (Class 435/124)
-
Patent number: 5210033Abstract: Disclosed is a method for selecting a mutant strain belonging to the genus Streptomyces which is a hyper-producer of Streptovaricin C superior to those know heretofore. This is accomplished by first subjecting a natural strain of Streptomyces spectabilis to conditions so as to isolate organisms which are streptovaricin resistant. The streptovaricin resistant organisms thus isolated are then subjected to mutagenesis and then cultured. The colonies which are asporogenous are individually cultured in fermentation batches such that the strains take the form of pellets of varying sizes and colors. From the batch having the most heterogeneous mixture of pellets, the smallest pellet or the pellet(s) having the deepest color (usually deep red or crimson) is isolated. We have discovered that the strain of this pellet has a high likelihood of being a hyperproducer of streptovaricin. The strain of this pellet may then be subjected to fermentation conditions to produce streptovaricin.Type: GrantFiled: April 23, 1992Date of Patent: May 11, 1993Assignees: Shin-Etsu Bio, Inc., Shin-Etsu Chemical Co., Ltd.Inventors: Kaname Inoue, Motohide Yamazaki, Kanji Murofushi, Richard W. Armentrout
-
Patent number: 5190866Abstract: A method for separation and recovery of polymeric beads from an antibiotic fermentation broth comprising suspending a mixture of the beads and any inherent mold from the fermentation in an aqueous solution having a specific gravity which is effective to cause the beads to form a discrete layer at or on the surface of the solution, separate and apart from the mold. The separate layer of the beads may then be easily removed from the liquid by a conventional physical methods.Type: GrantFiled: January 27, 1992Date of Patent: March 2, 1993Assignee: Shin-Etsu Bio, Inc.Inventors: Kaname Inoue, Motohide Yamazaki, Richard W. Armentrout
-
Patent number: 5171676Abstract: The fungus Beauveria sulfurescens has been employed to introduce hydroxyl groups into artemisinin and derivatives of artemisinin, an antimalarial. Hydroxylated derivatives of artemisinin and derivatives of artemisinin produced thereby possess antimalarial activity, and can serve as intermediates in the synthesis of further derivatives useful in treating malaria.Type: GrantFiled: October 31, 1991Date of Patent: December 15, 1992Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Herman Ziffer, Yulin Hu
-
Patent number: 5156961Abstract: A method for separation and recovery of polymeric beads from an antibiotic fermentation broth comprising suspending a mixture of the beads and any inherent mold from the fermentation in an aqueous solution having a specific gravity which is effective to cause the beads to form a discrete layer at or on the surface of the solution, separate and apart from the mold. The separate layer of the beads may then be easily removed from the liquid by a conventional physical methods.Type: GrantFiled: October 23, 1990Date of Patent: October 20, 1992Assignee: Shin-Etsu Bio, Inc.Inventors: Kaname Inoue, Motohide Yamazaki, Richard W. Armentrout
-
Patent number: 5126254Abstract: A method for producing streptovaricin by culturing a streptovaricin producing strain in the presence of a nonionic adsorbent. This procedure substantially increases the production efficiency of the streptovaricin. A second embodiment wherein the streptovaricin producing strain is produced in the presence of fumaric acid or its water soluble salts to provide improvements in production efficiency is also disclosed.Type: GrantFiled: October 23, 1990Date of Patent: June 30, 1992Assignees: Shin-Etsu Chemical Co., Ltd., The Institute of Physical Chemical ResearchInventors: Isao Endo, Shigehiro Nagura, Kaname Inoue, Jun Watanabe
-
Patent number: 5112742Abstract: The present invention relates to the method of screening for antibiotics employing a cyanobacterium, Nostoc muscorum A. The cyanobacterium is photoinduced under specified growing conditions to enable the cyanobacterium to be employed for screening of chemicals or organisms that exhibit anti-adhesin properties. These anti-adhesin properties are characterized by zones of interference in the photoinduced lawns of the cyanobacterium.Type: GrantFiled: February 23, 1990Date of Patent: May 12, 1992Assignee: State University of New YorkInventor: Norman Lazaroff
-
Patent number: 5104798Abstract: A microbiological process for the oxidation of methyl groups in aromatic 5- or 6-member ring heterocycles to the corresponding carboxylic acid. The heterocycle serves as the substrate and exhibits no substituent on the carbon atoms adjacent to the methyl groups to be oxidized. The reaction of the heterocycle takes place by means of microorganisms of the genus Pseudomonas utilizing toluene, xylene, or cymene. The enzymes of the microorganisms have been previously induced.Type: GrantFiled: February 5, 1991Date of Patent: April 14, 1992Assignee: Lonza Ltd.Inventor: Andreas Kiener
-
Patent number: 5102793Abstract: A microbiological process for sterospecific monoketo reduction of racemic 3-keto-bicyclic carbacyclin intermediates to the corresponding optically active 30.alpha. hydroxy compounds is provided.Type: GrantFiled: July 13, 1988Date of Patent: April 7, 1992Assignee: Schering AktiengesellschaftInventors: Karl Petzoldt, Helmut Dahl, Helmut Vorbruggen
-
Patent number: 5084388Abstract: A method of producing a brassinosteroid is disclosed, wherein crown gall cells capable of producing the brassinosteroid are cultured in a culture medium in the presence of a cultivation aid selected from auxins, sterols, squalene, casamino acid and mixtures thereof.Type: GrantFiled: December 18, 1990Date of Patent: January 28, 1992Assignees: Somar Corporation and The Institute of Physical and Chemical Research, The Institute of Physical and Chemical ResearchInventors: Hiroshi Saimoto, Masako Otsuka, Mifumi Yamamoto, Masao Kawashima, Shozo Fujioka, Akira Sakurai, Takao Yokota, Kunihiko Shono
-
Patent number: 5045457Abstract: Compounds of formula (II): ##STR1## wherein R.sup.3 is optionally protected hydroxy; one of R.sup.4 and R.sup.5 is optionally protected hydroxy; and the other of R.sup.4 and R.sup.5 is hydrogen, which are obtainable by the fermentation of a Streptomyces microorganism, have anthelmintic activity.Type: GrantFiled: January 23, 1989Date of Patent: September 3, 1991Assignee: Beecham Group p.l.c. of Beecham HouseInventors: Rhona M. Banks, Simon E. Blanchflower, Peter R. Shelley
-
Patent number: 5026642Abstract: In a process for the reduction of ketones, the keto compounds are enantioselectively reduced to give secondary alcohols by means of microorganisms. Keto compounds of the formulaR.sup.3 NH--CHR.sup.1 --CO--CH.sub.2 --COOR.sup.2in which R.sup.1, R.sup.2 and R.sup.3 have the meanings defined herein are used as starting compounds.Type: GrantFiled: July 22, 1988Date of Patent: June 25, 1991Assignee: Merck Patent Gesellschaft mit beschrankter HaftungInventors: Hans-Eckart Radunz, Harry Schwartz, Martin Heinrich
-
Patent number: 5008191Abstract: Compounds with acaricidal, insecticidal and anthelmintic activities have the formula: ##STR1## wherein: --X--Y-- is selected from the group consisting of --CH.sub.2 --CH.sub.2 --, --CH.sub.2 --CHOH--, --CH.dbd.CH--, and --CH.sub.2 --C(.dbd.O)--;R.sup.1 is selected from the group consisting of a methyl group, an ethyl group, an isopropyl group, a sec-butyl group and groups of formula --C(CH.sub.3).dbd.CHR.sup.5 in which R.sup.5 is selected from the group consisting of a methyl group, an ethyl group and an isopropyl group;R.sup.2 represents a group of formula --(CH.sub.2).sub.n --C(R.sup.6).dbd.C(R.sup.7)(R.sup.8) in which n is 0, 1 or 2, R.sup.6 and R.sup.7 each is selected from the group consisting of a hydrogen atom and a methyl group and R.sup.8 is selected from the group consisting of a hydrogen atom, a C.sub.1-4 alkyl group, a phenyl group and a phenyl group substituted with at least one substituent selected from the group consisting of halogen, methyl and nitro substitutents;R.sup.Type: GrantFiled: December 10, 1987Date of Patent: April 16, 1991Assignee: Sankyo Company LimitedInventors: Takao Okazaki, Shuji Takahashi, Seigo Iwado, Keiji Tanaka, Toshiaki Yanai, Hisaki Kajino
-
Patent number: 4946782Abstract: Described is a process for the preparation of compositions of matter containing unsaturated lactones defined according to the generic structure: ##STR1## wherein R represents C.sub.6 alkyl or alkenyl; and X represents C.sub.2, C.sub.4 or C.sub.6 alkylene or alkenylene; with the provisos that R is C.sub.6 alkyl when X is alkenylene and R is C.sub.6 alkenyl when X is alkylene by means of the sequential steps of (i) fermentation of castor oil or ricinoleic acid using a microorganism selected from the group consisting of:Candida petrophilum, ATCC 20226;Candida oleophila, ATCC 20177;Candida sp., ATCC 20504; andCandida sake, ATCC 28137whereby gamma hydroxydecanoic acid and a mixture of other acids defined according to the generic structure: ##STR2## is formed wherein Y represents an oxo-saturated, oxo-unsaturated or di-unsaturated C.sub.9, C.sub.11 or C.sub.Type: GrantFiled: August 5, 1988Date of Patent: August 7, 1990Assignee: International Flavors & Fragrances Inc.Inventors: Mohamad I. Farbood, James A. Morris, Mark A. Sprecker, Lynda J. Bienkowski, Kevin P. Miller, Manfred H. Vock, Myrna L. Hagedorn
-
Patent number: 4885246Abstract: New intermediate, an optically active (1S,5R)-7,7-alkylenedioxy-2-alkoxycarbonylbicyclo[3.3.0]octan-3-one which is useful for the synthesis of an optically active carbacyclin. This intermediate is prepared, as a non-reduced compound, from a racemic compound, (1SR,5RS)-7,7-alkylenedioxy-2-alkoxycarbonyl-cis-bicyclo[3.3.0]octan-3-one , by treatment of the racemic compound with a microorganism. The microorganism has an ability of specifically reducing the keto group of the (1R,5S) epimer of the racemic compound.Type: GrantFiled: December 19, 1988Date of Patent: December 5, 1989Assignee: Nisshin Flour Milling Co., Ltd.Inventors: Kenji Mori, Masahiro Tsuji
-
Patent number: 4681846Abstract: Process for the preparation of difficidin and derivative antibacterial compounds of the formula: ##STR1## where R.sub.a and R.sub.b are members independently selected from the group consisting of hydrogen; alkali metal and alkaline earth metal cations; ammonium; and substituted ammonium; and R.sup.1 is hydrogen or hydroxy.Type: GrantFiled: May 6, 1985Date of Patent: July 21, 1987Assignee: Merck & Co., Inc.Inventors: Kenneth E. Wilson, Richard L. Monaghan, Cheryl DeRiso, Sheldon B. Zimmerman, Otto D. Hensens, James E. Flor, Sagrario M. Del Val, Maria I. M. Fernandez
-
Patent number: 4609742Abstract: The subject disclosure discloses a chemical intermediate in antibiotic synthesis of the structure: ##STR1## wherein R.sub.1 is H or OH and the asterisks indicate asymmetric carbon atoms. Compounds of this type may be obtained by fermentation with a strain of B. subtilis under aerobic conditions. Compounds of the above structure are biologically inactive but are converted by phosphorylation of the hydroxyl group to produce compounds of the structure: ##STR2## wherein R.sub.1 is H or OH, which are useful antibacterial substances having a broad spectrum of antibacterial activity.Type: GrantFiled: February 4, 1985Date of Patent: September 2, 1986Assignee: Merck & Co., Inc.Inventors: Eugene D. Thorsett, Joanne M. Williamson, Kenneth E. Wilson
-
Patent number: 4536474Abstract: An undifferentiated symbiotic combination of alga and fungus cells, obtained directly from a lichen explant, are cultured in a media under appropriate conditions to produce aromatic lichenous substances and these substances are recovered.Type: GrantFiled: September 30, 1982Date of Patent: August 20, 1985Assignee: Nippon Paint Co., Ltd.Inventors: Yoshikazu Yamamoto, Ryuzo Mizuguchi, Yasuyuki Yamada
-
Patent number: 4480033Abstract: Yield of lankacidin antibiotics is enhanced by incorporating cyclodextrin into a culture medium where a microorganism capable of producing lankacidin is cultivated.Type: GrantFiled: April 6, 1983Date of Patent: October 30, 1984Assignee: Takeda Chemical Industries, Ltd.Inventors: Takashi Suzuki, Junya Okada, Hidekazu Sawada
-
Patent number: 4440857Abstract: A new microorganism, Streptomyces fradiae NRRL 12201, which produces mycarosyltylactone (5-O-mycarosyl-20-dihydro-20,23-dideoxytylonolide) and a process for preparing tylactone (20-dihydro-20,23-dideoxytylonolide) and mycarosyltylactone by submerged aerobic fermentation of this microorganism, or a mycarosyltylactone-producing mutant or recombinant thereof, are provided.Type: GrantFiled: March 18, 1982Date of Patent: April 3, 1984Assignee: Eli Lilly and CompanyInventors: Eugene T. Seno, Richard H. Baltz
-
Patent number: 4415669Abstract: There is disclosed a macrolide isolated from the fermentation broth of a microorganism identified as MA-5285 which morphological analysis reveals to be a strain of Streptomyces hygroscopicus. The compound's structure is presented based upon analytical studies. The compound has insecticidal and antiparasitic activity.Type: GrantFiled: December 7, 1981Date of Patent: November 15, 1983Assignee: Merck & Co., Inc.Inventor: Sebastian Hernandez
-
Patent number: 4385065Abstract: There is disclosed a novel series of compounds isolated from the fermentation broth of a strain of the microorganism Streptomyces sp MA5038. The compounds have a 16-membered macrocyclic lactone structure with various substituents thereon. The compounds have insecticidal and antitapeworm activity and compositions for such uses are disclosed.Type: GrantFiled: October 5, 1981Date of Patent: May 24, 1983Assignee: Merck & Co., Inc.Inventors: George E. Albers-Schonberg, Sebastian Hernandez, Leeyuan Huang
-
Patent number: 4385116Abstract: 2'"-O-demethylmacrocin (DOMM) which has the formula: ##STR1## 20-dihydro-DOMM, 2'"-O-demethyllactenocin (DOML), 20-dihydro-DOML, specified acyl ester derivatives, and their acid addition salts are useful antibacterial agents.Type: GrantFiled: July 15, 1980Date of Patent: May 24, 1983Assignee: Eli Lilly and CompanyInventors: Richard H. Baltz, Gene M. Wild, Eugene T. Seno
-
Patent number: 4366247Abstract: A process for preparing tylactone (20-dihydro-20,23-dideoxytylonolide), which has the formula: ##STR1## by submerged aerobic fermentation of Streptomyces fradiae NRRL 12188 or a tylactone-producing mutant or recombinant thereof is provided.Type: GrantFiled: July 2, 1980Date of Patent: December 28, 1982Assignee: Eli Lilly and CompanyInventors: Richard H. Baltz, Eugene T. Seno
-
Patent number: 4362881Abstract: Tylactone (20-dihydro-20,23-dideoxytylonolide), which has the formula: ##STR1## and specified acyl ester derivatives thereof are useful intermediates in the preparation of macrolide antibiotics.Type: GrantFiled: January 15, 1982Date of Patent: December 7, 1982Assignee: Eli Lilly and CompanyInventors: Robert L. Hamill, Gerald L. Huff, Richard H. Baltz, Eugene T. Seno
-
Patent number: 4239690Abstract: When Cytospora sp. is subjected to aerobic fermentation a number of new antibiotics are produced. Methods of production, recovery and purification of these antibiotics are described and some of their antimicrobial properties are described.Type: GrantFiled: October 22, 1979Date of Patent: December 16, 1980Assignee: Washington State University Research FoundationInventors: Sarangamat Gurusiddaiah, Robert C. Ronald, James A. Magnuson, Bruce A. McFadden
-
Patent number: 4220718Abstract: When Cytospora sp. is subjected to aerobic fermentation a number of new antibiotics are produced. Methods of production, recovery and purification of these antibiotics are described and some of their antimicrobial properties are described.Type: GrantFiled: March 1, 1978Date of Patent: September 2, 1980Assignee: Washington State University Research Foundation, Inc.Inventors: Sarangamat Gurusiddaiah, Robert C. Ronald, James A. Magnuson, Bruce A. McFadden