Preparing Nitrogen-containing Organic Compound Patents (Class 435/128)
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Publication number: 20150140617Abstract: The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ?-hydroxycarboxylic acids and ?-hydroxycarboxylic acid esters.Type: ApplicationFiled: January 30, 2015Publication date: May 21, 2015Applicant: EVONIK DEGUSSA GMBHInventors: Markus POETTER, Hans-Georg Hennemann, Steffen Schaffer, Thomas Haas
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Publication number: 20150132807Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.Type: ApplicationFiled: November 12, 2014Publication date: May 14, 2015Inventors: Nicholas J. Agard, Oscar Alvizo, Melissa Ann Mayo, Stefanie Ng Minor, James Nicholas Riggins, Jeffrey C. Moore
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Publication number: 20150132808Abstract: A mutant lysine decarboxylase is produced by replacing at least one of the amino-acids in the amino acid sequence shown in sequence ID No. 4 of the sequence listing with another amino-acid.Type: ApplicationFiled: January 17, 2013Publication date: May 14, 2015Inventors: Daisuke Mochizuki, Tadashi Araki, Akiko Natsuji, Tomomi Sakai, Tomonori Hidesaki
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Patent number: 9029106Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: June 18, 2013Date of Patent: May 12, 2015Assignee: Codexis, Inc.Inventors: Ish K. Dhawan, Gregory Miller, Xiyun Zhang
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Patent number: 9023624Abstract: The present invention is related to a method for the fermentative production of hydroxymethionine, comprising the steps of: culturing a recombinant microorganism modified to produce methionine in an appropriate culture medium comprising a source of carbon, a source of sulfur and a source of nitrogen, recovering hydroxymethionine from the culture medium. In a specific embodiment, the recombinant microorganism is cultivated under conditions of nitrogen limitation. The invention is also related to the biologically-produced hydroxymethionine and its uses.Type: GrantFiled: December 30, 2010Date of Patent: May 5, 2015Assignee: Metabolic ExplorerInventors: Rainer Figge, Wanda Dischert
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Publication number: 20150118721Abstract: The invention relates to novel enzymes that provide acetylated sphingoid bases.Type: ApplicationFiled: March 13, 2013Publication date: April 30, 2015Applicant: EVONIK INDUSTRIES AGInventors: Steffen Schaffer, Mike Farwick, Heiko Andrea, Tim Koehler, Daniel Wolff, Frank ter Veld, Ansgar Poetsch, Eckhard Boles, Christoph Schorsch
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Publication number: 20150111262Abstract: This document describes biochemical pathways for producing glutaric acid, 5-aminopentanoic acid, 5-hydroxypentanoic acid, cadaverine or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as D-proline.Type: ApplicationFiled: August 27, 2014Publication date: April 23, 2015Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20150111253Abstract: The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.Type: ApplicationFiled: March 12, 2013Publication date: April 23, 2015Applicant: EVONIK INDUSTRIES AGInventors: Steffen Schaffer, Michaela Hauberg, Mirja Wessel, Hans-Georg Hennemann, Jan Christoph Pfeffer, Thomas Haas, Harald Haeger
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Publication number: 20150104838Abstract: The present invention relates to a recombinant microorganism having enhanced ability of producing putrescine at high yield, which is generated by weakening the activity of NCgl1469 in a microorganism of Corynebacterium genus that is modified to produce putrescine, and a method for producing putrescine using the same.Type: ApplicationFiled: January 11, 2013Publication date: April 16, 2015Inventors: Su Jin Choi, Hyang Choi, Min Sun Kang, Sung Hoo Jhon, Kyoung Min Lee, Hye Won Um, Young Lyeol Yang
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Patent number: 8999452Abstract: The present invention provides a surface-independent surface-modifying multifunctional biocoating and methods of application thereof. The method comprises contacting at least a portion of a substrate with an alkaline solution comprising a surface-modifying agent (SMA) such as dopamine so as to modify the substrate surface to include at least one reactive moiety. In another version of the invention, a secondary reactive moiety is applied to the SMA-treated substrate to yield a surface-modified substrate having a specific functionality.Type: GrantFiled: July 11, 2013Date of Patent: April 7, 2015Assignee: Northwestern UniversityInventors: Phillip B. Messersmith, Haeshin Lee
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Patent number: 8999680Abstract: The present invention relates to a novel method for the preparation of 1,4-diaminobutane [DAB]. The method according to the present invention involves at least one biocatalytic step which comprises the biocatalytic production of at least one N-protected precursor of DAB. The present invention also relates to a method for the preparation of DAB involving at least one biocatalytic step, and comprising the steps of a) biocatalytically preparing an N-protected precursor of DAB yielding a—biocatalytic reaction mixture containing the N-protected precursor of DAB, b) recovering the N-protected precursor from the biocatalytic reaction mixture and c) converting the N-protected precursor into DAB. More in particular, the present invention relates to a method for the preparation of DAB, wherein the at least N-protected precursor of DAB is selected from the group consisting of N5-protected ornithine, N-protected DAB, and N-protected 4-aminobutyraldehyde.Type: GrantFiled: July 20, 2010Date of Patent: April 7, 2015Assignee: DSM IP Assets B.V.Inventors: Liang Wu, Petronella Catharina Raemakers-Franken
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Patent number: 8999681Abstract: A method of producing cadaverine is more efficient and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having a resistance to a pH of 5.5 or less. Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.Type: GrantFiled: December 8, 2011Date of Patent: April 7, 2015Assignee: Toray Industries, Inc.Inventors: Nanami Sasaki, Takashi Mimitsuka, Hideki Sawai, Kenji Sawai
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Publication number: 20150087028Abstract: The invention relates to recombinant expression of variant forms of M. thermophila CBH1a and homologs thereof, having improved thermoactivity, specific activity, and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.Type: ApplicationFiled: March 12, 2013Publication date: March 26, 2015Inventors: Behnaz Behrouzian, Xinkai Xie, Kui Chan, Xiyun Zhang, Vesna Mitchell, Douglas A. Hattendorf
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Publication number: 20150087031Abstract: The present invention relates to methods of processing lignocellulosic material to obtain hemicellulose sugars, cellulose sugars, lignin, cellulose and other high-value products. Also provided are hemicellulose sugars, cellulose sugars, lignin, cellulose, and other high-value products.Type: ApplicationFiled: May 3, 2013Publication date: March 26, 2015Inventors: Robert Jansen, Claire Gregoire, philip Travisano, Lee Madsen, Neta Matis, Yael Har-Tal, Shay Eliahu, James Alan Lawson, Noa Lapidot, Luke Burke, Aharon M. Eyal, Timothy Allen Bauer, Hagit Sade, Paul Mcwilliams, Ziv-Vladimir Belman, Bassem Hallac, Michael Zviely, Yelena Gershinksy, Adam Carden
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Patent number: 8986948Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.Type: GrantFiled: July 1, 2009Date of Patent: March 24, 2015Assignee: Lesaffre et CompagnieInventors: Dawid Brat, Eckhard Boles, Marco Keller, Beate Wiedemann
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Publication number: 20150079640Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.Type: ApplicationFiled: November 19, 2014Publication date: March 19, 2015Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff
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Patent number: 8980578Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: GrantFiled: June 10, 2013Date of Patent: March 17, 2015Assignee: Codexis, Inc.Inventors: Ryan Fong, Xiyun Zhang, Chris Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
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Publication number: 20150057465Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.Type: ApplicationFiled: March 17, 2014Publication date: February 26, 2015Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D. LYNCH
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Publication number: 20150057461Abstract: The present invention relates to a transformant which is transformed to express Baeyer-Villiger monooxygenase (BVMO), a method for producing C5-C14 medium-chain ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids, or alcohols from C16-C20 long-chain fatty acids by biotransformation using the transformant, a method for producing a fatty acid derivative having an ester group which is introduced into the chain thereof from keto fatty acid using the BVMO, and novel ?-hydroxy fatty acids which are prepared by the method. Degradation products such as C5 to C14 ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids, alcohols can be produced in a large amount from C16 to C20 long-chain fatty acids contained in a medium by biotransformation using a transformant capable of expressing BVMO of the present invention. Therefore, it can be widely used to produce ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids or alcohols in a more safe and economic manner.Type: ApplicationFiled: April 5, 2013Publication date: February 26, 2015Inventors: Jin Byung Park, Ji Won Song, Eun Yeong Jeon
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Publication number: 20150056665Abstract: A method of producing a chemical includes culturing cells in a culture solution in a fermentor to ferment a feedstock to produce a chemical; supplying the culture solution containing the chemical produced in the culturing to a plurality of separation membrane units arranged in parallel; filtering the culture solution supplied in the supplying to separate a permeate containing the chemical; refluxing a retentate that is not filtered in the filtering to the fermentor; and supplying a gas containing oxygen to the plurality of separation membrane units while a supply amount is changed to at least two different values to perform scrubbing, wherein the supply amount and supply time of the gas containing oxygen supplied in the culturing and the supplying the gas are set so that a kLa value is within a predetermined range from an optimal kLa value for the cells cultured in the culturing.Type: ApplicationFiled: March 27, 2013Publication date: February 26, 2015Inventors: Satoko Kanamori, Hideki Sawai, Norihiro Takeuchi
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Publication number: 20150056669Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.Type: ApplicationFiled: March 17, 2014Publication date: February 26, 2015Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D LYNCH
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Publication number: 20150050706Abstract: Disclosed is a process for carrying out a cyclisation reaction, a polymerization reaction, an enzymatic reaction showing substrate inhibition, an enzymatic reaction showing product inhibition, a reaction showing precipitation of the substrate or of a reactant, the process comprising the steps of a) diluting a fresh substrate with solvent to form a diluted substrate-solvent mixture, and supplying this mixture to a reactor, b) causing the reaction medium in the reactor to react, c) discharging reaction mixture comprising reaction product, solvent, and substrate that has not reacted, to a first filtration membrane which is permeable to the solvent and impermeable to the substrate and to the catalyst or at least one of the reactants, d) returning solvent from the permeate side of the first membrane to dilute the fresh substrate, and e) returning retentate comprising substrate which has not reacted, from the first filtration membrane to the reactor.Type: ApplicationFiled: April 19, 2013Publication date: February 19, 2015Inventors: Anita Buekenhoudt, Pieter Vandezande, Dominique Ormerod
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Publication number: 20150031094Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.Type: ApplicationFiled: October 1, 2014Publication date: January 29, 2015Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt W. Huisman, Steven J. Collier
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Publication number: 20150031077Abstract: The efficient production of ethanol from low-cost biomass (e.g., corn, sugar beets, sugar cane, switchgrass and/or paper) has become increasingly important in making ethanol competitive with gasoline and decreasing the United States' dependence on foreign oil. For example, to reduce the cost of transporting biomass to ethanol production facilities, mobile systems for producing ethanol from biomass are provided. Also provided are small-scale ethanol production facilities. For example, instead of transporting biomass to the production facility, the facility is transported to the biomass or is located nearby the source of the biomass. The ethanol production facilities or components thereof may be transported via land, water, or air. Production of other products, such as hydrocarbons, natural gas, hydrogen gas, plastics, polymers, and proteins, can also be made by the methods and facilities. Any product described herein can be made in finished form or un-finished form and moved, e.g., to a fixed facility, e.g.Type: ApplicationFiled: September 9, 2014Publication date: January 29, 2015Inventor: Marshall Medoff
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Publication number: 20150031095Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.Type: ApplicationFiled: October 2, 2014Publication date: January 29, 2015Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
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Publication number: 20150031076Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: ApplicationFiled: October 2, 2014Publication date: January 29, 2015Inventors: Hubertus Johannes Marie OP DEN CAMP, Harry Ramanoedj HARHANGI, Christiaan VAN DER DRIFT, Jacobus Thomas PRONK
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Patent number: 8940516Abstract: The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants (Arabidopsis) from which this enzyme or its gene is isolated is also described as non-cyanogenic. All HNL-containing plants described so far are cyanogenic plants and so it has until now been assumed that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly, it transpires that a polypeptide (AtHNL) of the invention is (R)-selective. The amino acid sequence gives a theoretical molecular weight of 29.2 kDa for the AtHNL subunit. The calculated molecular mass of the protein of approximately 30 kDa can be confirmed by SDS gel electrophoresis.Type: GrantFiled: November 19, 2012Date of Patent: January 27, 2015Assignee: Evocatal GmbHInventors: Thorsten Eggert, Jennifer Andexer
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Patent number: 8932836Abstract: The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine and its analogs, which can product compounds can be further used to prepare the aminocyclohexylether compound, (3R)-1-[(1R,2R)-2-[2-(3,4-dimethoxyphenyl)ethoxy]cyclohexyl]pyrrolidin-3-ol, which is an ion channel blocker.Type: GrantFiled: August 8, 2011Date of Patent: January 13, 2015Assignee: Codexis, Inc.Inventors: John Limanto, Gregory Beutner, Brendan Grau, Jacob Janey, Artis Klapars, Eric R. Ashley, Hallena R. Strotman, Matthew D. Truppo, Gregory Hughes, Fabien Cabirol, Anupam Gohel, Steven J. Collier, Jack Liang, Marissa Mock, Emily Mundorff, Scott Novick, Derek Smith
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Patent number: 8932835Abstract: A process for the enantioselective enzymatic reduction of a keto compound of general formula I wherein R may represent any protective group for amino functions (tert. butyloxycarbonyl group (BOC), benzyloxycarbonyl group, 9-fluorenylmethoxycarbonyl group) and X?—Cl, —CN, —OH, Br, F.Type: GrantFiled: September 22, 2008Date of Patent: January 13, 2015Assignee: IEP GmbHInventors: Antje Gupta, Maria Bobkova, Anke Tschentscher
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Patent number: 8932837Abstract: Process for producing optically active 3-aminocarboxylic acid ester compounds of general Formula I, and the ammonium salts thereof, in which R1 stands for alkyl, alkoxyalkyl, alkenyl, cycloalkyl, heterocycloalkyl, aryl, or hetaryl, and R2 stands for alkyl, cycloalkyl or aryl, in which an enantiomeric mixture of a simply N-acylated 3-aminocarboxylic acid ester of general formula (I.b), in which R1 and R2 have the meanings given above and R3 stands for hydrogen, alkyl, cycloalkyl or aryl, is submitted to an enantioselective deacylation by adding a polypeptide according to claim 1.Type: GrantFiled: July 15, 2013Date of Patent: January 13, 2015Assignee: BASF SEInventors: Bernhard Hauer, Thomas Friedrich, Rainer Stürmer, Nina Schneider, Susanne Sabrowski, Wolf-Rüdiger Krahnert
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Patent number: 8932838Abstract: The present disclosure provides engineered transaminase polypeptides having improved properties as compared to naturally occurring transaminases including the ability of converting the substrate, 3?-hydroxyacetophenone to (S)-3-(1-aminoethyl)-phenol in enantiomeric excess and high percentage conversion. Also provided are polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to synthesize (S)-3-(1-aminoethyl)-phenol and related compounds useful in the production of active pharmaceutical ingredients.Type: GrantFiled: August 19, 2014Date of Patent: January 13, 2015Assignee: Codexis, Inc.Inventors: Fabien Louis Cabirol, Anupam Gohel, Seong Ho Oh, Derek J. Smith, Brian Wong, James J. Lalonde
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Patent number: 8927774Abstract: The present invention relates to a method for separating and purifying 1,4-diaminobutane at high purity and high yield from a fermented solution comprising 1,4-diaminobutane, through cell mass removement, desalination, concentration, impurities removal, and recovery. Also, provided is a method for separating and purifying 1,4-diaminobutane at high purity and high yield from a fermented solution 1,4-diaminobutane, through cell mass removement, desalination, low-temperature concentration, crystallization, filtration, high-temperature concentration and distillation.Type: GrantFiled: August 24, 2012Date of Patent: January 6, 2015Assignee: CJ Cheiljedang CorporationInventors: Won Sik Gwak, Soon Won Hong, Soo An Shin, Han Won Lee
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Publication number: 20150004660Abstract: Embodiments of the invention relate to the enzymatic conversion of bioderived feedstocks to commercially valuable chemicals. The enzymatic conversions of the embodiments of the invention offer the potential for lower cost routes to these value-added chemicals. Some of the chemicals that are useful include nylon intermediates such as caprolactam, adipic acid, 1,6-hexamethylene diamine; butanediols such as 1,4-butanediol, 1,3-butanediol, and 2,3-butanediol; butanols such as 1-butanol, and 2-butanol; succinic acid, butadiene, isoprene, and 3-hydroxypropanoic acid.Type: ApplicationFiled: September 18, 2014Publication date: January 1, 2015Inventors: Paul S. Pearlman, Changlin Chen, Adriana Leonora Botes
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Patent number: 8921079Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.Type: GrantFiled: June 21, 2010Date of Patent: December 30, 2014Assignee: Codexis, Inc.Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff
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Publication number: 20140377815Abstract: The present invention relates to a method of producing carbamoyl phosphate, the method comprising reacting ammonia, ATP, bicarbonate and CO2, or a hydrated form thereof, in a composition in the presence of a carbamate kinase, wherein the ammonia and CO2, or hydrated form thereof, are converted to carbamate in a chemical reaction and the carbamate and ATP are converted to carbamoyl phosphate in an enzyme-catalysed reaction by the carbamate kinase, and wherein the pH of the composition is about 8 to about 12. The invention also relates to methods of producing urea.Type: ApplicationFiled: June 15, 2012Publication date: December 25, 2014Inventors: James Edward Hennessy, Amy Philbrook, Daniel Miles Bartkus, Christopher John Easton, Colin Scott, John G. Oakeshott, Hye-Kyung Kim, Melissa Jane Latter
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Patent number: 8916364Abstract: The invention relates to a nitrilase having improved activity in the reaction of a nitrile to form the corresponding carboxylic acid, in particular with respect to reacting 2-methylglutaronitrile, 1-(cyanomethyl)cyclohexane-1-carbonitrile, and benzonitrile. The nitrilase according to the invention is related to nitrilase from acidovorax facilis.Type: GrantFiled: October 12, 2011Date of Patent: December 23, 2014Assignee: c-LEcta GmbHInventors: Andreas Vogel, Daniel Schwarze, Thomas Greiner-Stoeffele
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MICROORGANISMS AND METHODS FOR PRODUCTION OF 4-HYDROXYBUTYRATE, 1,4-BUTANEDIOL AND RELATED COMPOUNDS
Publication number: 20140371417Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.Type: ApplicationFiled: April 25, 2014Publication date: December 18, 2014Applicant: Genomatica, Inc.Inventors: Priti PHARKYA, Anthony P. BURGARD, Stephen J. VAN DIEN, Robin E. OSTERHOUT, Mark J. BURK, John D. TRAWICK, Michael P. KUCKINSKAS, Brian STEER -
Patent number: 8911831Abstract: The invention provides surface treatments that reduce or eliminate marine biofouling of various surfaces. A surface that is to be subjected to a marine environment can be treated with a mPEG-DOPA. The treated surface is thus rendered less susceptible to fouling of the surface.Type: GrantFiled: June 3, 2010Date of Patent: December 16, 2014Assignee: Northwestern UniversityInventors: Phillip B. Messersmith, Haeshin Lee, Andrea R. Statz, Bruce P. Lee, Jeffrey L. Dalsin, Daniel Sherman
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Publication number: 20140364579Abstract: The present invention provides a method for conveniently and inexpensively producing an amino-hydroxybenzoic acid-type compound such as 3-amino-4-hydroxybenzoic acid by utilizing Escherichia coli that is a bacterium commonly used for a production process by biosynthesis. Specifically, the present invention provides an Escherichia coli having an ability to produce 3-amino-4-hydroxybenzoic acid, which is modified to reduce an activity of N-hydroxyarylamine O-acetyltransferase (NhoA); a method for producing a 3-amino-4-hydroxybenzoic acid-type compound using such Escherichia coli; and the like.Type: ApplicationFiled: August 25, 2014Publication date: December 11, 2014Applicant: AJINOMOTO CO., INC.Inventors: Fumi Shozui, Yoshinori Tajima
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Publication number: 20140363859Abstract: The present invention relates to a recombinant microorganism having enhanced ability to produce putrescine at high yield, wherein the activity of NCgl0101 is weakened in a microorganism of genus Corynebacterium that has been modified to produce putrescine, and a method for producing putrescine using the same.Type: ApplicationFiled: January 21, 2013Publication date: December 11, 2014Inventors: Kyoung Min Lee, Seon Hye Kim, Hye Won Um, Min Sun Kang, Su Jin Choi, Hee Kyoung Jung, Sung Hoo Jhon, Hongxian Li, Won Sik Gwak, Chong Ho Lee, Young Lyeol Yang
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Patent number: 8906653Abstract: The invention relates to a method for isolating 1,5-diaminopentane (DAP) from DAP-containing fermentation broths, to a method for the fermentative production of DAP using said isolating method and to a method for producing DAP-comprising polymers by using the DAP isolated or fermatively produced DAPs according to said methods.Type: GrantFiled: January 23, 2009Date of Patent: December 9, 2014Assignee: BASF SEInventors: Martin Völkert, Oskar Zelder, Burkhard Ernst, Weol Kyu Jeong
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Publication number: 20140356916Abstract: Recombinant microorganisms with deregulated succinyl-CoA synthetase activity, as well as the uses for producing lysine, ?-lysine, cadaverine or N-acetylcadaverine thereby are provided. Recombinant polypeptides comprising an amino acid sequence being at least 80% identical to SEQ ID NO: 51 or 53 are also provided. The method of producing fine chemicals using said recombinant microorganisms, in particular the method of producing lysine, or derivatives thereof, such as ?-lysine, cadaverine or N-acetylcadaverine is further provided.Type: ApplicationFiled: December 14, 2012Publication date: December 4, 2014Applicant: BASF SEInventors: Christoph Wittmann, Stefanie Kind, Judith Becker
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Patent number: 8895285Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.Type: GrantFiled: July 12, 2012Date of Patent: November 25, 2014Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Takanori Akiyama, Wataru Mizunashi, Tetsuji Nakamura
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Publication number: 20140342412Abstract: The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.Type: ApplicationFiled: September 7, 2012Publication date: November 20, 2014Applicant: CODEXIS, INC.Inventors: Fabien Cabirol, Haibin Chen, Anupam Gohel, Paulina Salim, Derek Smith, Jacob Janey, Birgit Kosjek, Weng Lin Tang, Helen Hsieh, Son Pham
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Patent number: 8889380Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: September 5, 2012Date of Patent: November 18, 2014Assignee: Codexis, Inc.Inventors: Christopher Savile, Emily Mundorff, Jeffrey Moore, Paul N. Devine, Jacob M. Janey
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Publication number: 20140335570Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: May 8, 2014Publication date: November 13, 2014Applicant: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8883475Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.Type: GrantFiled: October 23, 2012Date of Patent: November 11, 2014Assignee: Codexis, Inc.Inventors: Christopher Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman
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Patent number: 8877475Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.Type: GrantFiled: September 11, 2012Date of Patent: November 4, 2014Assignee: Codexis, Inc.Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
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Publication number: 20140322769Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.Type: ApplicationFiled: November 14, 2012Publication date: October 30, 2014Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
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Patent number: 8871477Abstract: By a method for producing cadaverine by culturing a microorganism that extracellularly secretes lysine decarboxylase, by-production of lysine is suppressed, the yield of cadaverine relative to glucose consumption is improved compared to conventional production methods, and further, the load on the purification step in purification of cadaverine as a raw material for polyamide can be reduced.Type: GrantFiled: February 22, 2011Date of Patent: October 28, 2014Assignee: Toray Industries, Inc.Inventors: Takashi Mimitsuka, Kazumi Suda, Hideki Sawai