Carboxylic Acid Ester Patents (Class 435/135)
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Patent number: 9023626Abstract: The invention relates to a method for the synthesis of diacids of general formula ROOC—(CH2)n—(CH?CH)a—(CH2)mCOOR1 in which n and m are identical or different and each represent an integer such that their sum is between 6 and 15, a is an index of 0 or 1, and R and R1 are H or an alkyl radical with 1 to 4 carbon atoms, from long-chain mono-unsaturated natural fatty acids or esters having at least 10 adjacent carbon atoms per molecule of the general formula CH3—(CH2)p—CH?CH—(CH2)q—COOR, p and q, being identical or different and representing indices between 2 and 11, wherein said method comprises the first step of oxidizing by fermentation said natural fatty acid or ester, using a microorganism, such as a bacterium, a yeast, or a fungus, into at least one unsaturated dicarboxylic acid or dicarboxylate, the second step of submitting the product from the first step to a metathesis crossed with a compound of formula R2OOC—(CH2)x—CH?CH—R3, in which R2 is H or an alkyl radical with 1 to 4 carbon atoms, x is 0 or 1 orType: GrantFiled: September 17, 2008Date of Patent: May 5, 2015Assignee: Arkema FranceInventor: Jean-Luc Dubois
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Publication number: 20150112037Abstract: The invention includes a process of producing methyl methacrylate or derivatives thereof is described. The process includes the steps of converting 2-butanone to methyl propionate using a Baeyer-Villiger monooxygenase, and treating the methyl propionate produced to obtain methyl methacrylate or derivatives thereof. A method of preparing polymers or copolymers of methyl methacrylate or its derivatives is also described.Type: ApplicationFiled: May 24, 2013Publication date: April 23, 2015Inventors: Graham Ronald Eastham, David William Johnson, Adrianus Johannes Jozef Straathof, Marco Wilhemus Fraaije, Remko Tsjibbe Winter
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Patent number: 8993269Abstract: Glucosyl stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The short-chain glucosyl stevia compositions were purified to >95% content of total steviol glycosides. The compositions can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.Type: GrantFiled: November 6, 2013Date of Patent: March 31, 2015Assignee: PureCircle USA Inc.Inventor: Avetik Markosyan
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Publication number: 20150087034Abstract: The present invention aims to provide a method for producing polybutylene terephthalate (PBT) with an excellent color using biomass-derived 1,4-butanediol (BG). The invention relates to a method for producing PBT comprising a step of subjecting a diol component containing raw material 1,4-BG having a nitrogen content of 0.01 to 50 ppm by mass and a dicarboxylic acid component to esterification or ester-exchange reaction, and a polycondensation reaction step for obtaining PBT from the reactant, wherein the content of gamma butyrolactone in the raw material 1,4-BG is 1 to 100 ppm by mass.Type: ApplicationFiled: December 4, 2014Publication date: March 26, 2015Applicants: MITSUBISHI CHEMICAL CORPORATION, GENOMATICA, INC.Inventors: Masaru UTSUNOMIYA, Yusuke IZAWA, Norikazu KONISHI, Shinichiro MATSUZONO, Takayuki SUZUKI, Michael JAPS, Mark BURK, Warren CLARK
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Publication number: 20150087802Abstract: A method for producing polyhydroxyalkanoate (PHA) comprises the steps of culturing in a culture medium comprising pyrolysis wax obtained from the pyrolysis of polyethylene and optionally a surfactant one or more bacterial strains which are capable of accumulating PHA from pyrolysis wax, and recovering the PHA from the culture medium. Typically, the bacterial strains are selected from the group consisting of: A. calcoaceticus BD413; A. calcoaceticus RR8; B. sepacia RR10; P. aeruginosa 3924; P. aeruginosa GL-1; P. aeruginosa PAO1; P. aeruginosa RR1; and P. olevorans.Type: ApplicationFiled: June 26, 2014Publication date: March 26, 2015Inventors: Kevin O'Connor, Maciej GUZIK, Ramesh PADAMATI, Shane KENNY
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Publication number: 20150079641Abstract: The invention relates to an isolated nucleotide sequence encoding an amino acid sequence that is at least ?90%, ?92%, ?94%, ?96%, ?97%, ?98%, ?99% or 100%, preferably ?97%, particularly preferably ?98%, very particularly preferably ?99%, and extremely preferably 0%, identical to the amino acid sequence of SEQ ID NO:2, wherein SEQ ID NO:2, at position 553, or at a corresponding position of the amino acid sequence, has a proteinogenic amino acid other than L-tyrosine, to a microorganism comprising the nucleotide sequence and also to a process for producing fine chemicals using this microorganism.Type: ApplicationFiled: April 12, 2013Publication date: March 19, 2015Applicant: EVONIK INDUSTRIES AGInventors: Robert Gerstmeir, Iris Wiegrabe
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Publication number: 20150079646Abstract: The present invention relates to yeast cells producing high levels of acetoacetyl-CoA. It also relates to a method for making such yeast cells and to the use of such yeast cells in a method for producing acetyl-CoA derived products.Type: ApplicationFiled: December 17, 2012Publication date: March 19, 2015Applicant: FIRMENICH SAInventors: Jens Nielsen, Verena Siewers, Yun Chen, Laurent Daviet, Michel Schalk
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Publication number: 20150080547Abstract: The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy) phosphonate (2H3M40P) pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce 2H3M40P, p-toluate or terephthalate. Also provided herein are processes for isolating bio-based aromatic carboxylic acid, in particular, p-toluic acid or terephthalic acid, from a culture medium, wherein the processes involve contacting the culture medium with sufficient carbon dioxide (C02) to lower the pH of the culture medium to produce a precipitate comprised of the aromatic carboxylic acid.Type: ApplicationFiled: January 18, 2013Publication date: March 19, 2015Applicant: Genomatica, Inc.Inventors: Robin E. Osterhout, Anthony P. Burgard, Mark J. Burk
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Patent number: 8980594Abstract: The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.Type: GrantFiled: October 11, 2010Date of Patent: March 17, 2015Assignee: Evonik Roehm GmbHInventors: Liv Reinecke, Steffen Schaffer, Tim Koehler, Anja Thiessenhusen, Achim Marx, Markus Buchhaupt
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Patent number: 8980587Abstract: A bacterium belonging to the genus Streptomyces having an ability to produce reveromycin A or a synthetic intermediate thereof, the bacterium being modified so as to increase expression of revQ gene coding for the amino acid sequence of SEQ ID NO: 36 or an amino acid sequence having an identity of not less than 80% to SEQ ID NO: 36 as compared with a parent strain, thereby the above-mentioned production ability is increased as compared with the parent strain.Type: GrantFiled: August 30, 2011Date of Patent: March 17, 2015Assignee: RIKENInventors: Hiroyuki Osada, Shunji Takahashi, Makoto Kawatani, Yoshiyuki Sakaki, Atsushi Toyoda
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Patent number: 8980592Abstract: In a process for the enantioselective enzymatic reduction of a hydroxy ketone of general formula I wherein R1=C1-C6 alkyl and R2=—Cl, —CN, —OH, —H or C1-C6 alkyl, into a chiral diol of general formula II wherein R1 and R2 have the same meaning as in formula I, the hydroxy ketone is reduced with an oxidoreductase in the presence of NADH or NADPH as a cofactor, wherein a) the hydroxy ketone is provided in the reaction at a concentration of ?50 g/l, b) the oxidized cofactor NAD or NADP having formed is regenerated continuously by oxidation of a secondary alcohol of general formula RXRYCHOH, wherein RX, RY independently represent hydrogen, branched or unbranched C1-C8-alkyl and Ctotal?3, and c) the reduction of the hydroxy ketone and the oxidation of the secondary alcohol are catalyzed by the same oxidoreductase.Type: GrantFiled: December 14, 2006Date of Patent: March 17, 2015Assignee: Cambrex IEP GmbHInventors: Antje Gupta, Maria Bobkova, Anke Tschentscher
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Patent number: 8980593Abstract: This invention relates to the production of short-chain polyhydroxyalkanoate biopolymers which include C3 to C5 3-hydroxyacyl monomers. The process includes culturing a native microorganism capable of synthesizing polyhydroxyalkanoate biopolymers using as a carbon source an amount of glycerol, levulinic acid and mixtures thereof that drives the microbial synthesis of polyhydroxyalkanoate biopolymers.Type: GrantFiled: November 16, 2012Date of Patent: March 17, 2015Assignee: The United States of America, as represented by the Secretary of AgricultureInventors: Richard D Ashby, Daniel K. Y. Solaiman, Gary D Strahan
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Patent number: 8980615Abstract: Aliphatic polyester productivity is improved for production of aliphatic polyester using a recombinant microorganism. A recombinant microorganism prepared by introducing a gene encoding a protein having activity of converting lactic acid to lactic-acid CoA and a gene encoding a protein having activity of synthesizing polyhydroxyalkanoate using hydroxyacyl CoA as a substrate into a host microorganism is cultured and then aliphatic polyester is recovered from the medium.Type: GrantFiled: March 24, 2011Date of Patent: March 17, 2015Assignee: Toyota Jidosha Kabushiki KaishaInventors: Masayoshi Muramatsu, Hiromi Kambe, Masakazu Ito, Takashi Shimamura, Katsunori Kohda
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Patent number: 8980253Abstract: Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.Type: GrantFiled: April 26, 2011Date of Patent: March 17, 2015Assignees: aTyr Pharma, Inc., Pangu BioPharma LimitedInventors: Leslie Ann Greene, Kyle P. Chiang, Fei Hong, Alain Philippe Vasserot, Wing-Sze Lo, Jeffry D. Watkins, Cheryl L. Quinn, John D. Mendlein
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Publication number: 20150072385Abstract: Several embodiments of the invention relate generally to a system and methods for the treatment of gaseous emissions comprising methane and one or more non-methane compounds that can influence the metabolism of methane-oxidizing microorganisms. In several embodiments, there is provided a system and methods for the treatment of methane emissions through the use of methanotrophic microorganisms to generate functionally consistent and harvestable products. Certain embodiments of the invention are particularly advantageous because they reduce environmentally-destructive methane emissions and produce harvestable end-products.Type: ApplicationFiled: October 28, 2014Publication date: March 12, 2015Inventors: Markus Donald Herrema, Kenton Kimmel
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Publication number: 20150072384Abstract: Microorganism compositions are described that comprise combinations of genetic modifications that include a genetic modification to increase oxaloacetate alpha-decarboxylase enzymatic activity. By such genetic modification a 3-hydroxypropionic acid (“3-HP”) production pathway is provided or improved.Type: ApplicationFiled: July 25, 2014Publication date: March 12, 2015Inventors: Michael D. Lynch, Christopher P. Mercogliano
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Publication number: 20150064751Abstract: The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage.Type: ApplicationFiled: November 14, 2014Publication date: March 5, 2015Applicants: The Board of Trustees of the Leland Stanford Junior University, GreenLight Biosciences, Inc.Inventor: James R. Swartz
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Publication number: 20150064750Abstract: The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.Type: ApplicationFiled: March 26, 2014Publication date: March 5, 2015Applicant: Genomatica, Inc.Inventors: Robin E. OSTERHOUT, Anthony P. Burgard, Priti Pharkya, Mark J. Burk
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Patent number: 8969051Abstract: A process for producing an optically active 2-alkyl-1,1,3-trialkoxycarbonylpropane (2), comprising a step of asymmetric hydrolysis of 2-alkyl-1,1,3-trialokoxycarbonylpropane (1) by using an enzyme capable of selectively hydrolyzing an ester moiety of either one enantiomer of 2-alkyl-1,1,3-trialkoxycarbonylpropane (1), or by using a culture of a microorganism capable of producing the enzyme or a treated object thereof.Type: GrantFiled: May 9, 2008Date of Patent: March 3, 2015Assignee: Sumitomo Chemical Company, LimitedInventors: Norihiko Hirata, Kazuhiro Yamauchi
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Publication number: 20150057465Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.Type: ApplicationFiled: March 17, 2014Publication date: February 26, 2015Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D. LYNCH
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Publication number: 20150056673Abstract: The invention concerns a microorganism which is genetically modified so as to i) synthesize a hydrocarbon monomer by fermentation of a carbon source, and ii) depolymerize a polymer constituted at least by hydrocarbon monomer which it is capable of synthesizing. The invention also concerns a method for producing a hydrocarbon monomer using a genetically modified microorganism of this type, as well as the coculture of this microorganism with another microorganism which is capable of synthesizing a polymer of interest.Type: ApplicationFiled: March 27, 2013Publication date: February 26, 2015Inventor: Cédric Boisart
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Publication number: 20150056669Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.Type: ApplicationFiled: March 17, 2014Publication date: February 26, 2015Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D LYNCH
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Patent number: 8962300Abstract: The present invention provides reagents and methods for biomaterial production from cyanobacteria.Type: GrantFiled: June 6, 2013Date of Patent: February 24, 2015Assignee: Arizona Board of Regents, a Body Corporate Acting for and on Behalf of Arizona State UniversityInventors: Hatem Mohammed, Wim Vermaas
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Patent number: 8956836Abstract: The present invention provides a simpler and more energy efficient process for the preparation of fatty acid methyl ester (biodiesel) from sun dried whole seed capsules of Jatropha curcas integrated with value addition of seed shells, deoiled cake and crude glycerol co-product stream. More specifically, the invention relates to a method of dispensing with the need for excess methanol recovery through distillation, cost-effective resin treatment for the refining of methyl ester and utilization of co-streams for preparation of high density energy briquettes and Polyhydroxyalkanoate biodegradable polymer in efficient and cost-effective manner.Type: GrantFiled: March 29, 2010Date of Patent: February 17, 2015Assignee: Council of Scientific and Industrial ResearchInventors: Pushpito Kumar Ghosh, Sandhya Chandrika Prasad Mishra, Mahesh Ramniklal Gandhi, Sumesh Chandra Upadhyay, Parimal Paul, Pritpal Singh Anand, Kiritkumar Mangaldas Popat, Anupama Vijaykumar Shrivastav, Sanjiv Kumar Mishra, Neelam Ondhiya, Ramesh Dudabhai Maru, Gangadharan Dyal, Harshad Brahmbhatt, Vinod Boricha, Doongar Ram Chaudhary, Babulal Rebary, Krushnadevsingh Sukhdevsinh Zala
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Patent number: 8956835Abstract: Methods are provided for producing biodegradable polyhydroxyalkanoates (PHAs) with desired geometry, molecular mass, mechanical and/or physical-chemical properties from glycerol, an inexpensive carbon source and byproduct of the biodiesel industry. Microorganisms capable of converting carbon to PHA can be used to convert biodiesel-glycerol to poly-3-hydroxybutyrate (PHB) or other monomer or copolymer PHAs via fermentation. The microorganisms are cultured in a medium comprising glycerol as a primary carbon source and one or more low molecular mass organic acids as a secondary carbon source. Biomass can be harvested from the culture medium and crude PHA extracted and purified, thereby recovering purified PHA with the desired property. After PHA isolation, a nucleating agent can be added to improve certain physical-chemical properties of the PHA, e.g., crystallization temperature, to enhance performance of the PHA during injection molding.Type: GrantFiled: November 23, 2011Date of Patent: February 17, 2015Assignee: Suny Research FoundationInventors: James P. Nakas, Chengjun Zhu, Joseph A. Perrotta, Christopher T. Nomura
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Publication number: 20150031098Abstract: Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E. coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E. coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.Type: ApplicationFiled: October 7, 2014Publication date: January 29, 2015Inventors: Si Jae PARK, Taek Ho YANG, Hye Ok KANG, Sang Hyun LEE, Eun Jung LEE, Tae Wan KIM
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Publication number: 20150031095Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.Type: ApplicationFiled: October 2, 2014Publication date: January 29, 2015Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
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Patent number: 8940509Abstract: The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.Type: GrantFiled: November 2, 2012Date of Patent: January 27, 2015Assignee: Genomatica, Inc.Inventors: Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya, Mark J. Burk
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Publication number: 20150025247Abstract: Esters of O-substituted hydroxy carboxylic acids are provided having Formula 1, or 2, or both Formulas 1 and 2: wherein R and R1 are independently selected from the group consisting of substituted and unsubstituted, branched- and straight-chain, saturated, unsaturated, and polyunsaturated C1-C22 alkyl, substituted and unsubstituted C3-C8 cycloalkyl, substituted and unsubstituted C6-C20 carbocyclic aryl, and substituted and unsubstituted C4-C20 heterocyclic; wherein the heteroatoms are selected from sulfur, nitrogen, and oxygen; and wherein n is 1-6. Process of producing esters of O-substituted hydroxy carboxylic acids are also provided.Type: ApplicationFiled: July 30, 2014Publication date: January 22, 2015Inventors: Liu DENG, Neil Warren BOAZ, Sabine DELAIRE
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Publication number: 20150025237Abstract: The present disclosure provides compounds and methods that are useful for the preparation of compounds useful as orexin-2 receptor antagonists.Type: ApplicationFiled: February 14, 2013Publication date: January 22, 2015Inventors: George Anthony Moniz, Annie Zhu Wilcoxen, Farid Benayoud, Jaemoon Lee, Huiming Zhang, Taro Terauchi, Ayumi Takemura, Yu Yoshida, Toshiaki Tanaka, Keiichi Sorimachi, Yoshimitsu Naoe, Yuji Kazuta
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Publication number: 20150024443Abstract: The invention relates to methods for preparing ingenol-3-angelate from ingenol or ingenol derivatives.Type: ApplicationFiled: July 25, 2014Publication date: January 22, 2015Inventors: Xifu Liang, Thomas Hogberg, Gunnar Grue-Sorensen, Thomas S. Moody, Andrew S. Rowan
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Patent number: 8936923Abstract: The invention relates to the production of biodiesel feedstock and biodiesel. A process for producing a biodiesel utilizing a thraustochytrid is provided.Type: GrantFiled: December 5, 2007Date of Patent: January 20, 2015Assignee: Biomass Research & Refining Pty LtdInventors: Lindsay Fisher, David Nicholls, Kevin Sanderson
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Patent number: 8936922Abstract: The present invention provides a novel approach for the preparation of fatty alcohol esters of ?-hydroxy carboxylic acids. In one form of the invention, the target fatty alcohol ester of ?-hydroxy carboxylic acid is produced by converting a lower alkyl ester of ?-hydroxy carboxylic acid into a fatty alcohol ester of ?-hydroxy carboxylic acid via alcoholysis (i.e., transesterification). The transesterification process is an equilibrium reaction, catalyzed chemically (i.e., with acids or bases) or enzymatically, that is shifted in the desired direction to produce the desired product. One way of shifting the reaction in the direction of the desired product is by reducing the concentration of one of the products (e.g., distillation of a lower-boiling alcohol as it is formed). Another preferred way of shifting the reaction in the direction of the desired product is by increasing the concentration of one of the reactants.Type: GrantFiled: April 11, 2012Date of Patent: January 20, 2015Assignee: Chemsmart, LLCInventors: Gerald S. Jones, Jr., Scott A. Goodrich, Joseph P. St. Laurent
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Publication number: 20150017683Abstract: Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described.Type: ApplicationFiled: December 19, 2012Publication date: January 15, 2015Inventors: Zia Abdullah, Michael L. Dickens, Micah Paul McCreery, Randy L. Jones, Elvin Ray Beach, III, Jon-David S. Sears, Erin Suzanne Schultz, Stephanie Ann Smith, Paul E. George, II
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Publication number: 20150010336Abstract: The present disclosure provides for enzymatic polymerization to produce polyester resins suitable for use in manufacturing toners. In embodiments, crystalline copolymers, which are polyesters, may be synthesized from lactones, cyclic anhydrides, cyclic carbonates, and combinations thereof. These crystalline copolymers, in turn, may be utilized in the synthesis of toner particles.Type: ApplicationFiled: September 20, 2014Publication date: January 8, 2015Inventors: Jordan H Wosnick, Santiago Faucher, Edward G Zwartz, Michael S Hawkins, Karen A Moffat
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Publication number: 20150010967Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material.Type: ApplicationFiled: September 24, 2014Publication date: January 8, 2015Inventors: Marshall Medoff, Thomas Craig Masterman
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Publication number: 20150004662Abstract: The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway or terephthalate pathway.Type: ApplicationFiled: September 12, 2014Publication date: January 1, 2015Inventor: Robin E. Osterhout
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Patent number: 8921083Abstract: A modified microorganism for producing a high yield of 1,4-butanediol, and a method of producing 1,4-butanediol using the modified microorganism, wherein the modified microorganism is altered so as to delete or disrupt expression of at least one of transcription regulatory factor NCg12886, GCN5-related N-acetyltransferase NCg12090, hypothetical protein NCg10224 or sugar phosphate isomerase/epimerase NCg12956.Type: GrantFiled: July 19, 2013Date of Patent: December 30, 2014Assignee: Samsung Electronics Co., Ltd.Inventors: Woo Yong Lee, Hyun Min Koo, Sung Soo Kim, Jae Young Kim, Jae Chan Park
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Patent number: 8921080Abstract: The invention relates to an isolated polypeptide having esterase activity comprising an amino acid sequence shown in any one of SEQ ID NO's 2, 4, 6, 8, 10, 12 or 14 or a homologue thereof, comprising an amino acid substitution or deletion of one or more amino acids as shown in said SEQ ID NO's and resulting in a mutant polypeptide having an increased concentration of the fraction of the mutant polypeptide being present as an active and soluble protein in cleared lysate of the mutant polypeptide expressed in E. coli relative to the concentration of the fraction of the polypeptide without the mutation being present as an active and soluble protein in cleared lysate of the polypeptide without the one or more deletion or substitution expressed in E. coli under the same conditions. The invention also relates to nucleic acid encoding the polypeptides according to the invention, and the use of the polypeptides.Type: GrantFiled: April 26, 2010Date of Patent: December 30, 2014Assignee: DSM IP Assets B.V.Inventors: Martin Kietzmann, Harald Pichler, Helmut Schwab, Amin El-Heliebi, Christine Winkler, Andreas Braun
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Publication number: 20140378646Abstract: The present application is directed at microorganisms of the genus Pseudomonas as deposited under DSM26199 (Pseudomonas sp. IPB-B26) and DSM26200 (Pseudomonas sp, N-128) with the Leibnitz Institute DSMZ. The present application is further directed at a process for the production of medium- and long-chain-length PHAs, comprising cultivating said microorganisms in a culture medium comprising a carbon source and isolating the PHA from the microorganisms. It has been observed that these microorganisms allow for efficient PHA production in high yields. In addition, the inventive microorganisms possess the valuable capability to incorporate unsaturated fatty acids into the resulting PHAs. The inventive microorganisms thus enable a later modification of the PHAs as well as cross-linking, thus opening new fields of applications for these materials.Type: ApplicationFiled: June 25, 2014Publication date: December 25, 2014Inventors: Monica Bassas Galia, Sagrario Arias Rivas, Gabriella Molinari, Kenneth Nigel Timmis
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Publication number: 20140377819Abstract: A process for producing hydroxyalkanoate copolymers, which comprises: (i) pre-treating a sucrose-containing feedstock in an acidic solution; (ii) feeding the pre-treated feedstock into a bioreactor containing polyhydroxyalkanoate producing microbial cells; (iii) cultivating the polyhydroxyalkanoate producing microbial cells to form a cell mass containing the hydroxyalkanoate copolymers; (iv) recoverying the hydroxyalkanoate copolymers from the cell mass. The pre-treating step has the main function of hydrolyzing sucrose into glucose and fructose, which in turn are converted into 4-ketovaleric acid to give a mixture of mono-saccharides and organic precursors for microbial synthesis of hydroxyalkanoate copolymers, and particularly of PHBVV ter-polymers. Complex and expensive purification processes of the substrates obtained from the pre-treating step are not needed. The solutions can be directly used as the feeding solutions for microbial PHA biosynthesis.Type: ApplicationFiled: November 17, 2011Publication date: December 25, 2014Applicant: BIO ON S.R.L.Inventor: Jian Yu
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Publication number: 20140378647Abstract: The present application is directed at a microorganism of the genus Pseudomonas as deposited under DSM26198 with the Leibnitz Institute DSMZ. The present application is further directed at a process for the production of medium- and long-chain PHAs, comprising cultivating said microorganism in a culture medium comprising a carbon source and isolating the PHA from the microorganism. It has been observed that the microorganism allows for PHA production in high yield. In addition, the inventive microorganism possesses the valuable capability to efficiently incorporate unsaturated and/or aromatically modified fatty acids into the resulting PHAs. Accordingly, the inventive microorganism enables the production of chemically diverse PHAs, opening new fields of applications for these materials.Type: ApplicationFiled: June 25, 2014Publication date: December 25, 2014Inventors: Monica Bassas Galia, Sagrario Arias Rivas, Gabriella Molinari, Kenneth Nigel Timmis
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Publication number: 20140377817Abstract: The present invention relates to microorganisms and polypeptides for detoxifying aldehydes associated with industrial fermentations. In particular, a heat-stable, NADPH- and iron-dependent alcohol dehydrogenase was cloned from Thermoanaerobacter pseudethanolicus 39E and displayed activity against a number of aldehydes including inhibitory compounds that are produced during the dilute-acid pretreatment process of lignocellulosic biomass before fermentation to biofuels. Methods to use the microorganisms and polypeptides of the invention for improved conversion of bio mass to biofuel are provided as well as use of the enzyme in metabolic engineering strategies for producing longer-chain alcohols from sugars using thermophilic, fermentative microorganisms.Type: ApplicationFiled: June 20, 2014Publication date: December 25, 2014Inventors: James G. Elkins, Sonya M. Clarkson
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MICROORGANISMS AND METHODS FOR PRODUCTION OF 4-HYDROXYBUTYRATE, 1,4-BUTANEDIOL AND RELATED COMPOUNDS
Publication number: 20140371417Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.Type: ApplicationFiled: April 25, 2014Publication date: December 18, 2014Applicant: Genomatica, Inc.Inventors: Priti PHARKYA, Anthony P. BURGARD, Stephen J. VAN DIEN, Robin E. OSTERHOUT, Mark J. BURK, John D. TRAWICK, Michael P. KUCKINSKAS, Brian STEER -
Patent number: 8911971Abstract: Glucosyl stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using starch as the source of glucose residues. The short-chain glucosyl stevia compositions were purified to >95% content of total steviol glycosides. The compositions can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.Type: GrantFiled: May 4, 2011Date of Patent: December 16, 2014Assignee: PureCircle USA Inc.Inventor: Avetik Markosyan
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Patent number: 8911977Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.Type: GrantFiled: March 13, 2013Date of Patent: December 16, 2014Assignee: E. I. du Pont de Nemours and CompanyInventors: Mark Scott Payne, Robert DiCosimo
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Patent number: 8901356Abstract: The present invention relates to methods of producing ingenol-3-angelate (I) from ingenol (II). Furthermore, the invention relates to intermediates useful for the synthesis of ingenol-3-angelate (I) from ingenol (II) and to methods of producing said intermediates.Type: GrantFiled: July 8, 2011Date of Patent: December 2, 2014Assignee: Leo Laboratories LimitedInventors: Thomas Högberg, Gunnar Grue-Sørensen, Xifu Liang, Anne Marie Horneman, Anders Klarskov Petersen
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Patent number: 8900834Abstract: A process for producing polylactic acid (PLA) comprising the steps: fermenting in a fermentation vessel a micro-organism of the genus Monascus in a medium at a pH less than or equal to 5 under conditions which produce lactic acid into the medium, converting the lactic acid produced into lactide, and polymerizing the lactide to form PLA.Type: GrantFiled: October 28, 2011Date of Patent: December 2, 2014Assignee: Total Research & Technology Feluy S.A.Inventors: Alexander Ruud Weusthuis, Emil Johan Harald Wolbert, Jan Springer, John Van Der Oost, Gerrit Eggink
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Patent number: 8900830Abstract: A process for efficiently producing optically active succinimide derivatives as key intermediates of (3R)-2?-(4-bromo-2-fluorobenzyl)spiro{pyrrolidine-3,4?(1?H)-pyrrolo[1,2-a]pyrazine}-1?,2,3?,5(2?H)-tetraone, which comprises the following reaction steps, and the step 2 is performed by using a non-animal-derived enzyme.Type: GrantFiled: July 28, 2011Date of Patent: December 2, 2014Assignees: Sumitomo Dainippon Pharma Co., Ltd., Kyowa Hakko Bio Co., Ltd.Inventors: Shinji Kita, Kazuya Tsuzaki, Eitora Yamamura
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Patent number: 8901254Abstract: This is to provide a process for preparing a polycarbonate diol diacrylate from a polycarbonate diol and a vinyl acrylate compound without using a protonic acid or a metal component. This is a process for preparing a polycarbonate diol diacrylate which comprises reacting a polycarbonate diol represented by the formula (I) and a vinyl acrylate compound represented by the formula (II) in the presence of a hydrolase, and a polycarbonate diol diacrylate represented by the formula (III) having a terminal acrylated ratio of 97% or more.Type: GrantFiled: March 28, 2011Date of Patent: December 2, 2014Assignee: Ube Industries, Ltd.Inventors: Youichi Yoshida, Yasuhito Yamamoto, Kenji Hirotsu