Carboxylic Acid Ester Patents (Class 435/135)
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Publication number: 20140349353Abstract: Methods and systems for producing prescribed unit size poly(3-hydroxyalkanoate) (PHA) polymers and copolymers are provided. The methods and systems can employ recombinant bacteria that are not native producers of PHA or lack enzymes to degrade PHA once synthesized, metabolize short to long chain fatty acids without induction, and express an (R)-specific enoyl-CoA hydratase and a PHA synthase, the (R)-specific enoyl-CoA hydratase and PHA synthase having wide substrate specificities. The recombinant bacteria are fed at least one fatty acid substrate that is equal in carbon length to the prescribed or desired unit size of the PHA polymer to be produced. The prescribed unit size PHA that is produced is then isolated and/or purified.Type: ApplicationFiled: January 9, 2013Publication date: November 27, 2014Applicant: The Research Foundation for the State University of New YorkInventors: Christopher T. Nomura, Ryan C. Tappel, Qin Wang
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Patent number: 8895272Abstract: Methods for producing a biofuel are provided. Also provided are biocatalysts that convert a feedstock to a biofuel.Type: GrantFiled: April 10, 2013Date of Patent: November 25, 2014Assignee: GEVO, Inc.Inventors: Andrew C. Hawkins, David A. Glassner, Thomas Buelter, James L. Wade, Peter Meinhold, Matthew W. Peters, Patrick R. Gruber, William A. Evanko, Aristos A. Aristidou, Marco Landwehr
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Publication number: 20140342413Abstract: A process for treating a mixture of R,R- and S,S-lactide is provided. The process involves contacting the lactide mixture with an aliphatic alcohol and/or an alkoxide to produce a mixture of R,R- and S,S-aliphatic ester of lactyllactic acid, subsequently contacting the mixture of R,R- and S,S-aliphatic ester of lactyllactic acid with an enzyme to produce a mixture comprising aliphatic ester of lactic acid corresponding to one lactide enantiomer and the aliphatic ester of lactyllactic acid corresponding to the other lactide enantiomer, and recovering the product. Also provided are processes for the production of S-lactic acid, S,S-lactide, poly-S-lactic acid, R-lactic acid, R,R-lactide, poly-R-lactic acid and stereocomplex polylactic acid.Type: ApplicationFiled: July 16, 2012Publication date: November 20, 2014Inventors: Edward Leslie Marshall, Jade Jocelyn Afriye Osei-Tutu, Stephen Alexander Calder Smith
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Patent number: 8889391Abstract: The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.Type: GrantFiled: September 12, 2011Date of Patent: November 18, 2014Assignee: Cargill, IncorporatedInventors: Holly Jean Jessen, Hans H. Liao, Steven John Gort, Olga V. Selifonova
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Patent number: 8883476Abstract: Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E. coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E. coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.Type: GrantFiled: July 26, 2013Date of Patent: November 11, 2014Assignee: LG Chem, Ltd.Inventors: Si Jae Park, Taek Ho Yang, Hye Ok Kang, Sang Hyun Lee, Eun Jung Lee, Tae Wan Kim
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Patent number: 8883463Abstract: There is provided a recombinant microorganism having producibility of poly(lactate-co-glycolate) from glucose, and more particularly, a recombinant microorganism having producibility of poly(lactate-co-glycolate) without adding an exogenous glycolate precursor, and a method of preparing [poly(preparing lactate-co-glycolate)] using the same. According to the present invention, the poly(lactate-co-glycolate) in which the concentration of the glycolate fraction is high may be prepared at a high concentration without supplying exogenous glyoxylate. Therefore, the present invention may be effectively used for treatment.Type: GrantFiled: March 9, 2012Date of Patent: November 11, 2014Assignees: Korea Advanced Institute of Science and Technology, Korea Research Institute of Chemical TechnologyInventors: Sang Yup Lee, Si Jae Park, Seung Hwan Lee, Bong Keun Song, Yu Kyung Jung, Tae Woo Lee
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Publication number: 20140329916Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.Type: ApplicationFiled: December 16, 2013Publication date: November 6, 2014Applicant: Genomatica, Inc.Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
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Publication number: 20140329980Abstract: The present invention provides multifunctional monomers, including, but not limited to include multifunctional methylene malonate and methylene beta-ketoester monomers; methods for producing the same; and compositions and products formed therefrom. The multifunctional monomers of the invention may be produced by transesterification or by direct synthesis from monofunctional methylene malonate monomers or methylene beta-ketoester monomers. The present invention further compositions and products formed from methylene beta-ketoester monomers of the invention, including monomer-based products (e.g., inks, adhesives, coatings, sealants or reactive molding) and polymer-based products (e.g., fibers, films, sheets, medical polymers, composite polymers and surfactants).Type: ApplicationFiled: October 18, 2012Publication date: November 6, 2014Inventors: Bernard M. Malofsky, Adam G. Malofsky, Tanmoy Dey, Larry Hines, Matthew M. Ellison
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Publication number: 20140329275Abstract: In one aspect, the invention provides a recombinant microbial cell modified to exhibit increased biosynthesis of a biosynthetic product compared to a wild-type control. In some cases, the biosynthetic product can be isocaproate; in other cases the biosynthetic product can be isovalerate. In another aspect, the invention provides methods of constructing such recombinant cells. In yet another aspect, the invention provides methods of using the cells to produce the biosynthetic product. In yet another aspect, the invention provides methods of harvesting organic acids from a fermentation culture.Type: ApplicationFiled: October 9, 2012Publication date: November 6, 2014Inventor: Kechun Zhang
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Patent number: 8877467Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material.Type: GrantFiled: March 6, 2013Date of Patent: November 4, 2014Assignee: Xyleco, Inc.Inventors: Marshall Medoff, Thomas Craig Masterman
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Patent number: 8865435Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.Type: GrantFiled: March 13, 2013Date of Patent: October 21, 2014Assignee: E. I. du Pont de Nemours and CompanyInventors: Mark Scott Payne, Robert DiCosimo
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Patent number: 8865437Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.Type: GrantFiled: March 13, 2013Date of Patent: October 21, 2014Assignee: E. I. du Pont de Nemours and CompanyInventors: Mark Scott Payne, Robert DiCosimo
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Patent number: 8865436Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.Type: GrantFiled: March 13, 2013Date of Patent: October 21, 2014Assignee: E. I. du Pont de Nemours and CompanyInventors: Mark Scott Payne, Robert DiCosimo
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Publication number: 20140308719Abstract: Embodiments of the present invention are directed to processes for the production of (R)-3-hydroxybutyl (R)-3-hydroxybyrate. Poly (R)-3-hydroxybyrate is transesterified with an alcohol, to form a first ester portion and a second ester portion. The first ester portion is reduced to the diol to form a diol portion and the diol portion is reacted with the second ester portion to produce (R)-3-hydroxybutyl (R)-3-hydroxybyrate.Type: ApplicationFiled: March 14, 2014Publication date: October 16, 2014Inventors: Kieran Clarke, Richard Lewis Veech, M. Todd King
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Patent number: 8859245Abstract: A lactic acid component (e.g., lactic acid or oligo (lactic acid)) can be obtained by extraction from a lactic acid fermentation liquor with a pH of 4.8 or less, using at least one solvent selected from the group consisting of toluene, xylene, mesitylene, ethylbenzene, methanol, ethanol, propanol, butanol, and mineral spirit. Furthermore, oligo (lactic acid) can be obtained, by heating a lactic acid fermentation liquor with a pH of 4.8 or less under reduced pressure, and washing, with water, the fermentation liquor containing a produced oligo (lactic acid). Hence, a method is provided for separating a lactic acid component from a lactic acid fermentation liquor, which is free from incorporation of impurities and which includes simple steps.Type: GrantFiled: March 8, 2007Date of Patent: October 14, 2014Assignees: Bio-Energy Corporation, Osaka University, Kansai Chemical Engineering Co., Ltd.Inventors: Hiroshi Uyama, Hideo Noda, Takahiko Terada
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Patent number: 8859246Abstract: The invention discloses a method for production of polyhydroxybutyrate-co-polyhydroxyvalerate (PHBV) by recombinant Escherichia coli harboring plasmid containing both phaCAB and prpE. Different percentage of hydroxyvalerate can be obtained from the recombinant E. coli when cultivated in the medium containing different concentrations of propionic acid. In this patent, we provide a method that integrated all of the genes (i.e. phaCAB, vgb and prpE) required for PHBV production into a single plasmid. The plasmids were then transformed into an E. coli host. Results showed that PHBV can be produced by this recombinant E. coli, and the ration of HV to HB in the co-polymers can be regulated by addition of different concentrations of propionic acid in the medium. The percentage of HV in the co-polymers can be adjusted from about 3% up to more than 35%.Type: GrantFiled: May 29, 2012Date of Patent: October 14, 2014Assignee: Yuan Ze UniversityInventors: Chih-Ching Chien, Po-Chi Soo, Yu-Tze Horng, Shan-Yu Chen, Hsiu-Hsiung Li, Yu-Hong Wei, Wen-Ming Chen
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Patent number: 8858657Abstract: A method and system for providing direct conversion of algal biomass. Optionally, the method and system can be used to directly convert dry algal biomass to biodiesels under microwave irradiation by combining the reaction and combining steps. Alternatively, wet algae can be directly processed and converted to fatty acid methyl esters, which have the major components of biodiesels, by reacting with methanol at predetermined pressure and temperature ranges.Type: GrantFiled: December 21, 2011Date of Patent: October 14, 2014Assignee: Arrowhead Center, Inc.Inventors: Shuguang Deng, Prafulla D. Patil, Veera Gnaneswar Gude
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Publication number: 20140302571Abstract: Carbon-containing materials, such as biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or coal are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol.Type: ApplicationFiled: June 20, 2014Publication date: October 9, 2014Inventors: Marshall MEDOFF, Thomas Craig MASTERMAN
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Publication number: 20140302572Abstract: An object of the present invention is to provide a polyhydroxyalkanoate which forms a useful bioplastic or biomaterial. Specifically, the present invention relates to a method for producing a polyhydroxyalkanoate that includes at least a 5-hydroxyvalerate unit, in which the method for producing a polyhydroxyalkanoate includes culturing a microorganism belonging to Ralstonia genus in a culture medium that includes lactone and/or hydroxy acid or salt of hydroxy acid as a carbon source.Type: ApplicationFiled: April 13, 2012Publication date: October 9, 2014Applicant: RIKENInventors: Keiji Numata, Miwa Yamada, Yoshiharu Doi
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Publication number: 20140295507Abstract: Disclosed is a method for producing biodegradable polymer and ethanol converted from carbon source by using recombinant microorganisms, comprising the steps of: (A) providing recombinant microorganisms transformed with plasmids containing at least a gene encoding for glycerol utilizing enzyme and a gene encoding for polyhydroxyalkanoate synthase; (B) culturing the recombinant microorganisms in a medium containing glycerol; (C) inducing expression of the genes of step (A), thereby obtaining polyhydroxyalkanoate and ethanol; and (D) recovering the polyhydroxyalkanoate and the ethanol; wherein the recombinant microorganisms have a glycerol utilization rate more than 90% (w/w), and have polyhydroxyalkanoate accumulated therein to a biomass content thereof at least 30% (w/w).Type: ApplicationFiled: June 11, 2014Publication date: October 2, 2014Inventors: Chi-Wei LAN, Ho-Shing WU, Feng-Shen CHIU
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Patent number: 8846353Abstract: Preparation of derivatives of ?-hydroxycarboxylic acid, including ?-hydroxycarboxylic acid esters, ?,?-unsaturated carboxylic acid, esters of ?,?-unsaturated carboxylic acid, and alkoxy derivatives, such as preparation of acrylic acid from 3-hydroxypropionic acid.Type: GrantFiled: December 18, 2012Date of Patent: September 30, 2014Assignee: Cargill, IncorporatedInventors: Paraskevas Tsobanakis, Xiangsheng Meng, Timothy Walter Abraham
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Patent number: 8841098Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.Type: GrantFiled: March 13, 2013Date of Patent: September 23, 2014Assignee: E.I. du Pont de Nemours and CompanyInventors: Mark Scott Payne, Robert DiCosimo
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Publication number: 20140273111Abstract: The invention relates to functionalized, telechelic polymers synthesized by enzymatic catalysis and methods, and the functionalization of polymers via Michael addition with a lipase catalyst, and the crosslinking of mono- or difunctional (telechelic) polymers made by enzymatic catalysis, such as by using multifunctional coupling agents and enzyme catalysts. Quantitative transesterification of vinyl methacrylate with poly(ethylene glycol), poly(isobutylene) and poly(dimethylsiloxane) was achieved using Candida antarctica lipase B. In addition, methacrylate-functionalized poly(ethylene glycol) monomethyl ether has been successfully coupled to aminoethoxy poly(ethylene glycol) monomethyl ether via Michael addition using Candida antarctica lipase B. Amine-functionalized poly(ethylene glycol)s have also been used for the preparation of poly(ethylene glycol)-based dendrimers and gels through Michael addition of the polymer onto triacryloyl hexahydro-triazine using the same enzyme.Type: ApplicationFiled: April 3, 2014Publication date: September 18, 2014Applicant: The University of AkronInventors: Judit E. Puskas, Mustafa Yasin Sen
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Publication number: 20140283219Abstract: The present invention provides methods of producing biological products or increasing production of such products through expression in a plant of a bacterial or plant glycolate catabolic cycle gene, such as glycolate dehydrogenease (GDH), glycolate oxidase (GO), or malate synthase (MS) in combination with a plant gene, such as farnesyl diphosphate synthase (FPS), squalene synthase (SQS), or PLAS. Also provided are plants, plants parts and compositions produced through methods of the present invention. The invention leads to two to five fold increase of end product yield.Type: ApplicationFiled: February 28, 2014Publication date: September 18, 2014Applicants: The Texas A&M University System, The University of Illinois at Urbana Champaign, University of KentuckyInventors: Shuhua (Joshua) Yuan, Donald Ort, Joseph Chappell, Xinguang Zhu, Hong Ma, Yong Kyoung Kim
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Publication number: 20140273115Abstract: A method is provided for controlling a metabolic profile of an anaerobic microbial fermentation culture. In particular, a metabolic profile of a fermentation process is controlled by controlling the amount of dissolved CO2 provided to a culture. Further provided is a method of producing one or more products by microbial fermentation of a gaseous substrate through feeding tail gas CO2 from a reactor to a second reactor, or by recycling tail gas CO2 to the same reactor.Type: ApplicationFiled: March 12, 2014Publication date: September 18, 2014Applicant: LanzaTech New Zealand LimitedInventors: Sean Dennis Simpson, Michael Koepke, Kathleen Frances Smart, Loan Phuong Tran, Paul Sechrist
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Publication number: 20140256004Abstract: The present invention relates to a novel process for the preparation of a compound of formula (I) wherein R is a methyl or methoxy group; to certain novel intermediates prepared in such a process and their use.Type: ApplicationFiled: October 19, 2012Publication date: September 11, 2014Applicant: CIPLA LIMITEDInventors: Dharmaraj Ramachandra Rao, Rajendra Narayanrao Kankan, Sanjay Naik, Maruti Ghagare, Sandip Vasant Chikhalikar
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Patent number: 8828691Abstract: The present disclosure provides a method for the stereospecific hydrolysis of racemic 1,1-dialkyloxycarbonylcyclopropanes.Type: GrantFiled: February 19, 2013Date of Patent: September 9, 2014Assignee: Bristol-Myers Squibb CompanyInventors: Animesh Goswami, Zhiwei Guo, Yuping Qiu
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Publication number: 20140242651Abstract: The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacylglycerol lipase mutants, as for example derived from Candida antarctica lipase B (CALB); a biocatalytic method of enantioselectively preparing an asymmetric mono-acylated polyol, catalyzed by the same enzyme mutants; as well as the use of a mutated triacylglycerol lipase in a method of preparing mono-acylated polyols. The invention also provides novel mutants, coding sequences thereof, and recombinant microorganisms carrying said coding sequences.Type: ApplicationFiled: March 20, 2014Publication date: August 28, 2014Applicant: BASF SEInventors: Bernhard Hauer, Cecilia Kvarnström Branneby, Karl Hult, Anders Magnusson, Anders Hamberg
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Publication number: 20140242650Abstract: Provided are a recombinant Ralstonia eutropha capable of producing polylactate or a hydroxyalkanoate-lactate copolymer, and a method of preparing polylactate or a hydroxyalkanoate-lactate copolymer using the same. The recombinant Ralstonia eutropha, which is prepared by introducing a gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate thereto, may be cultured, thereby efficiently preparing a lactate polymer and a lactate copolymer.Type: ApplicationFiled: December 18, 2013Publication date: August 28, 2014Applicant: LG CHEM, LTD.Inventors: Taek Ho YANG, Si Jae PARK, Eun Jeong LEE, Hye Ok KANG, Tae Wan KIM, Sang Hyun LEE
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Publication number: 20140242652Abstract: A method for producing succinic acid comprising (A) a step of converting an organic raw material to succinic acid in the presence of a microorganism or a processed product thereof in an aqueous medium and (B) a step of recovering the succinic acid, the method being characterized in that (A) the step of converting an organic raw material to succinic acid comprises: (a1) a step of adjusting the concentration of a succinic acid alkali metal salt in the aqueous medium to 80 mM or more; and then, (a2) a step of adding ammonia and/or an ammonium salt in the aqueous medium.Type: ApplicationFiled: May 12, 2014Publication date: August 28, 2014Applicant: MITSUBISHI CHEMICAL CORPORATIONInventors: Shuichi YUNOMURA, Daisuke Kido, Ryusuke Aoyama, Makoto Murase
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Publication number: 20140242647Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.Type: ApplicationFiled: February 20, 2014Publication date: August 28, 2014Applicant: California Institute of TechnologyInventors: Pedro S. Coelho, Eric M. Brustad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
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Publication number: 20140242649Abstract: The present invention provides for a system for converting CO2 and H2 to one or more biologically derived compounds. In some embodiments, the system comprises a host cell comprising one or more nucleic acids encoding genes for a recombinant surface display protein which is capable of tethering an electrocatalyst molecule, such as a cobalt(II) complex supported by tetradentate polypyridyl ligand 2-bis(2-pyridyl)(methoxy)methyl-6-pyridylpyridine (PY4), and enzymes for synthesizing a biologically derived compound, such as an alkane, alcohol, fatty acid, ester, or isoprenoid.Type: ApplicationFiled: May 8, 2014Publication date: August 28, 2014Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Yi-Chun Yeh, Steven W. Singer, Swapnil R. Chhabra, Harry R. Beller, Jana Mueller
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METHOD FOR OBTAINING AN OPEN PHOTOTROPHIC CULTURE WITH IMPROVED STORAGE COMPOUND PRODUCTION CAPACITY
Publication number: 20140242641Abstract: The invention is directed to a method for producing an open phototrophic culture with improved storage compound production capability. In accordance with the invention, a starting culture is submitted to selective pressure, thus giving a competitive advantage to storage compound producing species, by subjecting said starting culture to a cycle of alternating dark phases and light phases and providing limitation of availability of essential growth nutrients in one or more of said light phases. The resulting culture can be used to provide storage compounds in improved yields.Type: ApplicationFiled: July 18, 2012Publication date: August 28, 2014Applicant: Technische Universiteit DelftInventors: Jelmer Tamis, Robert Kleerebezem, Mark Cornelis Maria van Loosdrecht -
Publication number: 20140242640Abstract: The present invention is in the fields of nanotechnology and biomimetics. In particular, the present invention relates to the use of modified ribosomes to produce biomimetic structures. These biomimetic structures, also known as directed element polymers, are not produced by traditional industrial means but instead are produced by living systems comprising modified ribosomes.Type: ApplicationFiled: September 27, 2013Publication date: August 28, 2014Inventor: Alexander SUNGUROFF
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Patent number: 8815550Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.Type: GrantFiled: November 13, 2013Date of Patent: August 26, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Robert DiCosimo, Scott D Cunningham, Stephen R Fahnestock, Tanja Maria Gruber, Mark S Payne, Pierre E Rouviere, Linda Jane Solomon, Hong Wang
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Publication number: 20140234921Abstract: The present invention relates to novel polypeptides, or fragments of polypeptides, genes encoding them and means for producing said polypeptides. In detail the invention relates to polypeptides having esterase, suberinase and/or cutinase activity at low pH. This invention relates also to compositions containing the polypeptides and methods of using the polypeptides.Type: ApplicationFiled: April 11, 2012Publication date: August 21, 2014Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTTInventors: Antti Nyyssola, Hanna Kontkanen, Mari Hakkinen, Ville Pihlajaniemi, Markku Saloheimo, Johanna Buchert, Tiina Nakari-Setala
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Publication number: 20140235815Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.Type: ApplicationFiled: October 21, 2013Publication date: August 21, 2014Applicant: Genomatica, Inc.Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
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Publication number: 20140234922Abstract: A method of producing alkyl esters including processing a high free fatty acid feedstock including a mixture of triglycerides and free fatty acids to remove water and solids to create a processed feedstock. The processed feedstock is introduced and mixed into a reaction vessel, the reaction vessel includes water, at least one enzyme, and alcohol. The reacted contents are separated into a glycerin phase and an alkyl ester phase. The alkyl ester phase is treated with a primary alcohol and a flocculant.Type: ApplicationFiled: February 21, 2014Publication date: August 21, 2014Applicant: VIESEL SKUNK WORKS, LLCInventors: Graham Towerton, Stuart Lamb
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Patent number: 8809030Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: GrantFiled: October 22, 2012Date of Patent: August 19, 2014Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert Dicosimo, Mark Scott Payne, John Edward Gavagan
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Patent number: 8809015Abstract: Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes.Type: GrantFiled: October 21, 2011Date of Patent: August 19, 2014Assignee: LanzaTech New Zealand LimitedInventors: Michael Anthony Schultz, James Obern, Sean Dennis Simpson
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Publication number: 20140227745Abstract: The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.Type: ApplicationFiled: February 7, 2014Publication date: August 14, 2014Applicant: William Marsh Rice UniversityInventors: Ka-Yiu SAN, George N. BENNETT, Hui WU
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Patent number: 8802402Abstract: A substitution mutation that improves polymerization activity of a polyhydroxyalkanoic acid synthase is identified. At least 1 amino acid residue selected from the group consisting of a histidine residue at position 17, a proline residue at position 71, a valine residue at position 131, a methionine residue at position 205, a leucine residue at position 230, and a proline residue at position 239 of a polyhydroxyalkanoic acid synthase derived from Alcanivorax borkumensis is subjected to substitution mutation with another amino acid.Type: GrantFiled: July 14, 2010Date of Patent: August 12, 2014Assignee: Toyota Jidosha Kabushiki KaishaInventors: Masayoshi Muramatsu, Hiromi Kambe, Masakazu Ito
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Publication number: 20140206048Abstract: The present invention provides compositions and methods for the use of oil-containing materials as feedstocks for the production the bioproducts by biofermentation. In one preferred embodiment, surfactants are not used in compositions and the methods of the invention. In one preferred embodiment the oil-containing feedstocks are the by-products of other industrial processes including microbial, plant and animal oil processing.Type: ApplicationFiled: January 22, 2014Publication date: July 24, 2014Applicant: Glycos Biotechnologies, Inc.Inventors: Daniel J. Monticello, Ryan Black, Paul Campbell
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Publication number: 20140206049Abstract: Embodiments of the invention relate to the microbial production of polyhydroxyalkanoic acids, or polyhydroxyalkanoates (PHA), from substrates which cannot be used as a source of carbon and/or energy for microbial growth or PHA synthesis and which have microbial and environmental toxicity. According to one embodiment of the invention, a process for the production of PHA is provided wherein an enzyme such as methane monooxygenase is used to convert volatile organic compounds into PHA through the use of microorganisms that are unable to use volatile organic compounds as a source of carbon for growth or PHA production.Type: ApplicationFiled: March 21, 2014Publication date: July 24, 2014Applicant: NEWLIGHT TECHNOLOGIES, LLCInventors: Markus Herrema, Kenton Kimmel
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Publication number: 20140193868Abstract: The present invention is in the field of biosynthesis of polyhydroxyalkanoates (PHA). The invention relates to a genetically engineered microorganism having at least one gene involved in the metabolism, preferably in the production, of polyhydroxyalkanoates (PHA). This microorganism is useful in commercial production of polyhydroxyalkanoates. The present invention further relates to a method for the production of polyhydroxyalkanoates (PHA).Type: ApplicationFiled: January 7, 2014Publication date: July 10, 2014Inventors: Julia Sabirova, Peter Golyshin, Manuel Ferrer, Heinrich Lünsdorf, Wolf-Rainer Abraham, Kenneth Timmis
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Patent number: 8771720Abstract: Polyhydroxyalkanoates (PHAs) from which pyrogen has been removed are provided. PHAs which have been chemically modified to enhance physical and/or chemical properties, for targeting or to modify biodegradability or clearance by the reticuloendothelial system (RES), are described. Methods for depyrogenating PHA polymers prepared by bacterial fermentation processes are also provided, wherein pyrogens are removed from the polymers without adversely impacting the polymers' inherent chemical structures and physical properties. PHAs with advantageous processing characteristics, including low melting points and/or solubility in non-toxic solvents, are also described. The PHAs are suitable for use in in vivo applications such as in tissue coatings, stents, sutures, tubing, bone, other prostheses, bone or tissue cements, tissue regeneration devices, wound dressings, drug delivery, and for diagnostic and prophylactic uses.Type: GrantFiled: July 10, 2012Date of Patent: July 8, 2014Assignee: Metabolix, Inc.Inventors: Simon F. Williams, David P. Martin, Tillman Gerngross, Daniel M. Horowitz
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Publication number: 20140186910Abstract: Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.Type: ApplicationFiled: December 27, 2013Publication date: July 3, 2014Applicant: ButamaxTM Advanced Biofuels LLCInventors: LORI ANN MAGGIO-HALL, BRIAN JAMES PAUL, STEVEN CARY ROTHMAN, RICK W. YE
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Patent number: 8765425Abstract: Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity.Type: GrantFiled: March 23, 2012Date of Patent: July 1, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Robert Dicosimo, Arthur Leo Kruckeberg, Thomas Edwin Van Aken
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Patent number: 8759045Abstract: A method of producing lactic acid by separating lactic acid produced in a culture solution by microbial fermentation, comprising: a step (A) of filtering the culture solution through a nano-filtration membrane; and a step (B) of distilling a lactic-acid-containing solution produced in step (A) under a pressure of 1 Pa to atmospheric pressure (inclusive) at a temperature of 25 to 200° C. (inclusive) to recover lactic acid.Type: GrantFiled: December 7, 2012Date of Patent: June 24, 2014Assignee: Toray Industries, Inc.Inventors: Masateru Ito, Shin-ichi Minegishi, Eri Shimizu, Kenji Sawai, Yohito Ito, Hideki Sawai, Katsushige Yamada
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Patent number: 8759044Abstract: Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity.Type: GrantFiled: June 15, 2012Date of Patent: June 24, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Robert DiCosimo, Arthur Leo Kruckeberg, Thomas Edwin Van Aken