Involving Transferase Patents (Class 435/15)
  • Patent number: 10731200
    Abstract: A method of quantifying ammonia, which method includes: performing a first reaction in which a test liquid containing ammonia is reacted with ATP and L-glutamic acid in the presence of glutamine synthetase to produce ADP; performing a second reaction in which the produced ADP is reacted with glucose in the presence of ADP-dependent hexokinase to produce glucose-6-phosphate; performing a third reaction in which the produced glucose-6-phosphate is reacted with an oxidized NAD compound in the presence of glucose-6-phosphate dehydrogenase to produce a reduced NAD compound; and quantifying the reduced NAD compound to quantify ammonia.
    Type: Grant
    Filed: October 19, 2017
    Date of Patent: August 4, 2020
    Assignee: ARKRAY, Inc.
    Inventors: Takehiro Funamoto, Masaki Murakami
  • Patent number: 10724015
    Abstract: The present invention relates to a mutant microorganism in which a gene that encodes phosphofructokinase-2 is disrupted or deleted to reduce glycolytic flux to thereby improve the ability of the microorganism to produce N-acetylglucosamine, and to a method of producing N-acetylglucosamine using the mutant microorganism. The mutant microorganism according to the present invention has advantages in that it has high resistance to various chemical substances, grows rapidly, is easily cultured, and produces N-acetylglucosamine with high efficiency, indicating that it is useful for production of a large amount of N-acetylglucosamine.
    Type: Grant
    Filed: May 17, 2017
    Date of Patent: July 28, 2020
    Assignee: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION
    Inventors: Min-Kyu Oh, Sang-Woo Lee
  • Patent number: 10697870
    Abstract: An object is to provide a blood test kit and a blood analysis method capable of performing a blood test with a small amount of blood at high accuracy by visualizing a volume of blood collection and keeping the volume constant. Provided is a blood test kit for analyzing a concentration of a target component in a blood sample using a normal component homeostatically present in blood, the kit including: a blood collection instrument for collecting the blood sample; a diluent solution for diluting the collected blood sample; and a storing instrument for storing the diluted blood sample, in which the blood collection instrument is a capillary.
    Type: Grant
    Filed: January 3, 2018
    Date of Patent: June 30, 2020
    Assignee: FUJIFILM Corporation
    Inventors: Hikaru Urano, Tatsuya Ishizaka, Haruyasu Nakatsugawa, Susumu Osawa, Shinya Sugimoto
  • Patent number: 10688153
    Abstract: Modified peptides based on C-terminal PDZ binding domains of PTEN and PHLPP, or PDK1 interacting fragment of PKN2 are described as are methods of using the modified peptides for blocking the activity of PTEN, PHLPP and PKN2 and treating sudden cardiac arrest. A method for guiding treatment of cardiac arrest based on sorbitol or taurine levels is also provided.
    Type: Grant
    Filed: November 7, 2016
    Date of Patent: June 23, 2020
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Terry Vanden Hoek, Xiangdong Zhu, Jing Li
  • Patent number: 10677786
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of mucopolysaccharidosis (MPS) I or a disorder associated with MPS I.
    Type: Grant
    Filed: February 8, 2018
    Date of Patent: June 9, 2020
    Assignee: BIOMARIN PHARMACEUTICAL INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 10632150
    Abstract: Disclosed herein is a sterile composition for injection comprised of potassium phosphates having no visible particulate. Also disclosed herein is a manufacturing process for the sterile composition and its use.
    Type: Grant
    Filed: April 19, 2019
    Date of Patent: April 28, 2020
    Assignee: CMP Development LLC
    Inventors: H. Greg Thomas, Richard LeVasseur, Jeffrey S. Kiel, Anthony Reid Pipho
  • Patent number: 10619186
    Abstract: The disclosure provides for methods, compositions, and kits for normalizing nucleic acid libraries, for example sequencing libraries.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: April 14, 2020
    Assignee: Cellular Research, Inc.
    Inventors: Craig Betts, Glenn Fu
  • Patent number: 10596209
    Abstract: The present invention relates to the identification of a group of microorganisms, which are relatively abundant in the microbial communities associated with fruits and vegetables typically consumed raw and therefore transient or permanent members of the human microbiota. The consumption of mixtures of these microbes at relevant doses will produce a beneficial effect in the host by reducing the propensity to diabetes, obesity and metabolic syndrome mediated in part by production of short chain fatty acids to enhance colonic butyrate production. Therapeutic methods of the invention involve the use of live microorganisms or metabolites derived from said microorganisms to establish a microbial composition in the mammalian host that will improve significantly the ability to control weight, reduce the onset of diabetes, obesity and metabolic syndrome, and improve overall health.
    Type: Grant
    Filed: December 28, 2018
    Date of Patent: March 24, 2020
    Assignee: Solarea Bio, Inc.
    Inventors: Gerardo V. Toledo, Tracy Mincer, Jahir Mauricio Gutierrez Bugarin, Jillian DeWalt, Eric Schott, Maria Juliana Soto Giron
  • Patent number: 10590168
    Abstract: The invention provides kinase substrates and methods comprising their use.
    Type: Grant
    Filed: May 23, 2017
    Date of Patent: March 17, 2020
    Assignee: REGENTS OF THE UNIVERSITY OF MINNESOTA
    Inventors: Laurie L. Parker, Minervo Perez
  • Patent number: 10585270
    Abstract: A multi-plane imaging system for imaging multiple reflection planes of a regular or irregular shaped three-dimensional (3D) specimen having top, side and bottom views simultaneously. The system includes an inverted watertight pyramid well having at least four reflective side surfaces for reflecting the specimen side views, wherein each of the at least four reflective sides surfaces define an angle, ?, relative to the base horizontal plane, and wherein each reflective side surface comprises a plurality of reflective zones; A specimen is positioned horizontally equidistant from the at least four reflective side surfaces and positioned vertically from the base horizontal plane a predetermined vertical distance; wherein the predetermined vertical distance, the at least four reflective side surfaces, and the angle ? comprise a reflection image plane for reflecting the specimen bottom views.
    Type: Grant
    Filed: October 25, 2018
    Date of Patent: March 10, 2020
    Assignee: University of Vermont and State Agricultural College
    Inventors: Nathan R Tykocki, Grant Hennig
  • Patent number: 10570176
    Abstract: The present invention relates to the field of polypeptide medicine, and relates to polypeptides against hepatitis B virus X Protein and uses thereof. Specifically, the present invention relates to polypeptides comprising D-amino acids, which have a function of inhibiting hepatitis B virus X protein, and inhibit HBx activities, inhibit replication of hepatitis B virus DNA and expression of related antigens (e.g., HBeAg) at molecular level, cellular level and animal level, and further inhibit hepatitis and hepatic cirrhosis caused by hepatitis B virus infection and liver cancer that occurs on the basis of hepatic cirrhosis. The polypeptides can be widely used in the prevention and treatment of liver diseases after hepatitis B infection, including hepatitis, hepatic cirrhosis, and liver cancer.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: February 25, 2020
    Assignee: Tianjin TOPTECH Bio-Science & Technology Co., Ltd.
    Inventors: Xiaodong Zhang, Lihong Ye
  • Patent number: 10548948
    Abstract: Antifungal pharmaceutical compositions, dosage forms, and methods of use thereof are disclosed.
    Type: Grant
    Filed: February 27, 2018
    Date of Patent: February 4, 2020
    Assignee: The United States of America as represented by the Secretary of the Navy
    Inventors: Nancy Millenbaugh, Jeremy Wesley Gleaton, Dickson Kiprono Kirui
  • Patent number: 10475942
    Abstract: A photodetector cell may include a substrate, and a first contact carried by the substrate and having a first work function value. The photodetector cell may include a second contact carried by the substrate and having a second work function value different from the first work function value, and a semiconductor wire carried by the substrate and having a third work function value between the first and second work function values. The semiconductor wire may be coupled between the first and second contacts and comprising a photodiode junction.
    Type: Grant
    Filed: May 14, 2018
    Date of Patent: November 12, 2019
    Inventors: Manjeri P. Anantram, Md Golam Rabbani, Mahmoud M. Khader, Reza Nekovei, Amit Verma
  • Patent number: 10449182
    Abstract: The present invention provides sorafenib analogs for use in a method of treating a disease mediated by p21, said method comprising administering to a subject in need thereof a therapeutically effective amount of a compound of formula I. The present invention also provides methods of inhibiting p21 in a cell comprising contacting the cell with an effective amount of a compound of formula I.
    Type: Grant
    Filed: June 20, 2013
    Date of Patent: October 22, 2019
    Assignee: The Regents of the University of California
    Inventors: Robert H. Weiss, Bruce D. Hammock, Hiromi Inoue Wettersten, Sung Hee Hwang
  • Patent number: 10453554
    Abstract: The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: October 22, 2019
    Assignee: Codexis Mayflower Holdings, Inc.
    Inventor: Richard John Fox
  • Patent number: 10422006
    Abstract: Phosphatidylinositol 3-kinases (PI3Ks) are known to be important regulators of signaling pathways. To determine whether PI3Ks are genetically altered in cancers, we analyzed the sequences of the PI3K gene family and discovered that one family member, PIK3CA, is frequently mutated in cancers of the colon and other organs. The majority of mutations clustered near two positions within the PI3K helical or kinase domains. PIK3CA represents one of the most highly mutated oncogenes yet identified in human cancers and is useful as a diagnostic and therapeutic target.
    Type: Grant
    Filed: October 31, 2014
    Date of Patent: September 24, 2019
    Assignee: The John Hopkins University
    Inventors: Yardena Samuels, Victor Velculescu, Kenneth W. Kinzler, Bert Vogelstein
  • Patent number: 10405810
    Abstract: A tool for predicting that a person is likely to be abnormally hydrated over a future time interval, and in some cases to alert the person or a medical professional to intervene. From a series of physiological measurements, a tissue electrical impedance spectrum curve, which comprises a phase spectrum curve in an embodiment, is determined and changes in the shape of the curve are ascertained. The measurements may be received using one or more sensors worn by the person. In some embodiments, current and historic spectrum curvature are applied to an evolutionary algorithm, such as particle-swarm optimization (PSO) or differential evolution (DE), to determine an inference regarding the persons future hydration status. In one embodiment, a statistical forecast for the next epoch immediately beyond the present one, is determined.
    Type: Grant
    Filed: May 23, 2016
    Date of Patent: September 10, 2019
    Assignee: Cerner Innovation, Inc.
    Inventor: Douglas S. McNair
  • Patent number: 10407735
    Abstract: The present invention relates to compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present invention relates to ncRNAs as diagnostic markers and clinical targets for prostate, lung, breast and pancreatic cancer.
    Type: Grant
    Filed: May 17, 2016
    Date of Patent: September 10, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Arul Chinnaiyan, John Prensner, Matthew Iyer
  • Patent number: 10364425
    Abstract: The invention is related to a composition of recombinant or transgenic Factor VII, each molecule of Factor VII of the composition exhibiting two N-glycosylation sites, wherein, among all the molecules of FVII of the composition, the rate of Gal?1,3G al glycan moieties is comprised between 0 and 4%. The invention is also related to a process for preparing such a composition of FVII.
    Type: Grant
    Filed: May 2, 2013
    Date of Patent: July 30, 2019
    Assignee: LABORATOIRE FRANCAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES
    Inventors: Abdessatar Sami Chtourou, Emmanuel Nony, Nicolas Bihoreau
  • Patent number: 10316349
    Abstract: The present invention is a method for measuring the amount of at least one molecule in a biological sample, the method comprising a) combining the sample, or a derivative thereof, with one or more aptamers and allowing one or more molecules in the sample to bind to the aptamer(s); b) separating bound from unbound molecules; and c) quantifying the molecule(s) bound to the or each aptamer, wherein quantification of the bound molecule(s) is carried out by sequencing at least part of the or each aptamer. Uses of and products derived from the method are also contemplated.
    Type: Grant
    Filed: August 4, 2017
    Date of Patent: June 11, 2019
    Assignee: Caris Science, Inc.
    Inventors: Clive Gavin Brown, Koen Kas, Sven Agnes Jan Eyckerman
  • Patent number: 10294240
    Abstract: [Problem] To provide a fluorescent probe that detects calpain activity in cells at high sensitivity. [Solution] A compound represented by the following general formula (I) or a salt thereof.
    Type: Grant
    Filed: February 29, 2016
    Date of Patent: May 21, 2019
    Assignees: The University of Tokyo, Tohoku University
    Inventors: Yasuteru Urano, Yuri Nagayo, Mako Kamiya, Toru Nakazawa
  • Patent number: 10287590
    Abstract: The invention provides methods for introducing co-varying paired nucleic acids into a vector such under separate transcriptional control. An oligonucleotide is synthesized including the paired nucleic acids. The oligonucleotide is then assembled with a spacer nucleic acid encoding a promoter. After assembly the spacer nucleic acid is to one side of the oligonucleotide encoding the paired segments. However, on circularization of the assembled nucleic acid and cleavage between the DNA segments the spacer oligonucleotide and its components now occur between the paired segments. The resulting nucleic acid can now be cloned into a vector with a single step, such that each of the paired nucleic acid segments is linked to its own promoter. The present method can readily be extended to library screening without proportionately increasing the effort.
    Type: Grant
    Filed: December 24, 2014
    Date of Patent: May 14, 2019
    Assignee: DNA2.0, INC.
    Inventors: Jeremy Minshull, Sridhar Govindrajan, Kedar Patel
  • Patent number: 10287571
    Abstract: The present invention relates to a modified microorganism having, compared to its wildtype, —a reduced activity of an enzyme encoded by the ptsA-gene, —a reduced activity of an enzyme encoded by the ptsH-gene or —a reduced activity of an enzyme encoded by the ptsA-gene and a reduced activity of an enzyme encoded by the ptsH-gene, wherein the wildtype from which the modified microorganism has been derived belongs to the family of Pasteurellaceae. The present invention also relates to a method for producing succinic acid and to the use of modified microorganisms.
    Type: Grant
    Filed: May 7, 2015
    Date of Patent: May 14, 2019
    Assignee: BASF SE
    Inventors: Joanna Martyna Krawczyk, Stefan Haefner, Hartwig Schroeder, Oskar Zelder
  • Patent number: 10260084
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: April 16, 2019
    Assignee: BIOMARIN PHARMACEUTICAL INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 10233439
    Abstract: The present invention discloses a directionally modified glucosamine synthase mutant and its application. The amino acid sequence of the glucosamine synthase mutant is as shown in sequence list SEQ ID No. 1, and the nucleotide sequence is as shown in sequence list SEQ ID No. 2. The genetic engineering bacteria of glucosamine synthase is successfully constructed. In order to improve the tolerance of recombinant bacteria against glucosamine, the glucosamine synthase is directionally modified. A glucosamine synthase mutant is selected from the mutant library via high-throughput screening method, the amino acid changes in the mutant induces the spatial conformational change in the enzyme, so as enlarged the region where the enzyme and substrate combines, therefor the combination efficiency of the enzyme and the substrate is increased. The glucosamine synthase of the present invention has various advantages, such as rich in raw material of glucose, and a convenient subsequent extraction.
    Type: Grant
    Filed: June 5, 2017
    Date of Patent: March 19, 2019
    Assignees: Yangzhou Rixing Bio-Tech Co., Ltd, Jiangnan University
    Inventors: Jinsong Shi, Zhenghong Xu, Chao Zhang, Jinsong Gong, Heng Li, Zhenzhong Ding, Xiang Fang, Wanhong Zhang
  • Patent number: 10196692
    Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.
    Type: Grant
    Filed: September 18, 2015
    Date of Patent: February 5, 2019
    Assignee: QIAGEN MANCHESTER LIMITED
    Inventors: Ruth Board, Jennifer Haworth, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
  • Patent number: 10190171
    Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.
    Type: Grant
    Filed: September 18, 2015
    Date of Patent: January 29, 2019
    Assignee: QIAGEN MANCHESTER LIMITED
    Inventors: Ruth Board, Jennifer Haworth, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
  • Patent number: 10175226
    Abstract: A method for quantifying acetylamantadine in a urine sample comprises eluting acetylamantadine from the urine sample using solid phase extraction and quantifying the acetylamantadine eluted from the urine sample using Raman spectroscopy.
    Type: Grant
    Filed: July 10, 2017
    Date of Patent: January 8, 2019
    Assignee: BioMark Technologies, Inc.
    Inventors: Reuven Gordon, Brian Cheng, Rashid Bux, Bram Ramjiawan, Aftab Ahmed, Fraser Alan Hof
  • Patent number: 10077244
    Abstract: A method to detect the presence of amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: September 18, 2018
    Assignee: PROMEGA CORPORATION
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • Patent number: 10059955
    Abstract: The invention relates to isolated nucleic acids encoding a feruloyl-CoA:monolignol transferase and feruloyl-CoA:monolignol transferase enzymes. The isolated nucleic acids and/or the enzymes enable incorporation of monolignol ferulates into the lignin of plants, where such monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate. The invention also includes methods and plants that include nucleic acids encoding a feruloyl-CoA:monolignol transferase enzyme and/or feruloyl-CoA:monolignol transferase enzymes.
    Type: Grant
    Filed: October 4, 2016
    Date of Patent: August 28, 2018
    Assignees: Board of Trustees of Michigan State University, The University of British Columbia, Wisconsin Alumni Resarch Foundation
    Inventors: Curtis Wilkerson, John Ralph, Saunia Withers, Shawn D. Mansfield
  • Patent number: 10035974
    Abstract: The objective of the present invention is to provide a cleaning agent composition which can be used in the open air without any problems since the cleaning agent is environmentally friendly and which is effective for removing a low-molecular-weight and poorly-water-soluble cyclic silicone compound that causes a stain of a silicone sealing. The cleaning agent for washing out a silicone stain according to the present invention is characterized in comprising a cyclic lipopeptide biosurfactant, wherein the specific silicone stain compound such as D4 compound is the objective compound to be removed.
    Type: Grant
    Filed: March 25, 2014
    Date of Patent: July 31, 2018
    Assignees: KANEKA CORPORATION, NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Koji Noda, Masashi Izumida, Satohiro Yanagisawa, Tohru Nakashima, Takuto Nagano, Toshiaki Taira, Tomohiro Imura, Dai Kitamoto
  • Patent number: 9997656
    Abstract: A photodetector cell may include a substrate, and a first contact carried by the substrate and having a first work function value. The photodetector cell may include a second contact carried by the substrate and having a second work function value different from the first work function value, and a semiconductor wire carried by the substrate and having a third work function value between the first and second work function values. The semiconductor wire may be coupled between the first and second contacts and comprising a photodiode junction.
    Type: Grant
    Filed: December 17, 2015
    Date of Patent: June 12, 2018
    Inventors: Manjeri P. Anantram, Md Golam Rabbani, Mahmoud M. Khader, Reza Nekovei, Amit Verma
  • Patent number: 9982242
    Abstract: Provided are: a protein complex capable of selectively and asymmetrically oxidizing an enantiomer of a secondary alcohol without adding a coenzyme and having an asymmetric oxidation activity in a water-soluble solvent system in the presence of oxygen; a method for producing the same; and a method for coating the protein complex with a high molecular weight compound. The method for producing the protein complex includes: (1) enclosing a crude water-soluble protein in a gel, air-oxidizing the gel, and eluting the protein complex into an aqueous solution; and (2) applying gravity to concentrate and precipitate the protein complex, redissolving the precipitate in an aqueous glycine sodium hydroxide solution of about 0.5 mM and allowing the same to homogeneously coexist with a high molecular weight compound, and re-precipitating the solution and dehydrating and drying the same to yield a protein complex coated with a high molecular weight compound.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: May 29, 2018
    Assignee: SANYO FOODS CO., LTD.
    Inventor: Hiroyuki Nagaoka
  • Patent number: 9915649
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Grant
    Filed: April 12, 2016
    Date of Patent: March 13, 2018
    Assignee: BIOMARIN PHARMACEUTICALS INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 9896666
    Abstract: The present invention relates to a method for stabilizing an enzyme by storing the enzyme in the presence of a stable coenzyme. The present invention further relates to an enzyme stabilized with a stable coenzyme, and to the use thereof in test elements for detecting analytes.
    Type: Grant
    Filed: August 19, 2010
    Date of Patent: February 20, 2018
    Assignee: ROCHE DIABETES CARE, INC.
    Inventors: Carina Horn, Dieter Heindl, Claudia Gaessler-Dietsche, Joachim Hoenes
  • Patent number: 9879304
    Abstract: The present invention provides a method of quantifying the activity of a protein modifying enzyme in a sample, comprising: (i) grouping modified peptides from a first sample and modified peptides from a second sample into a single group according to one of the following parameters: (a) modified peptides having a modification site that is modified by the same protein modifying enzyme; or (b) modified peptides having a modification site that is part of the same modification motif; (ii) calculating enrichment of the modified peptides from the first sample compared to the modified peptides from the second sample in the group; and (iii) calculating the statistical significance of said enrichment; wherein a statistically significant enrichment is indicative of a protein modifying enzyme being activated in the first sample compared to the second sample. In some embodiments, the method further comprises identifying modified peptides in a first sample and a second sample using mass spectrometry (MS) prior to step (i).
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: January 30, 2018
    Assignee: Queen Mary & Westfield College, University of London
    Inventor: Pedro Rodriguez Cutillas
  • Patent number: 9874567
    Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enzymes. Kits may include an element tag for directly tagging an affinity product that recognizes phosphorylated substrates, as well as a non-phosphorylated substrate or an expression plasmid.
    Type: Grant
    Filed: February 2, 2016
    Date of Patent: January 23, 2018
    Assignee: Fluidigm Canada Inc.
    Inventor: Olga Ornatsky
  • Patent number: 9834807
    Abstract: Presented herein are solid supports on or in which a composition comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent) is present in added form. Presented herein are also methods for detecting the presence of an intracellular microorganism target (e.g., an intracellular bacterial target) utilizing such solid supports. Further, presented herein are tablets as well as dry powders comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent), and methods of using such tablets and dry powders to detect the presence of an intracellular microorganism target (e.g., an intracellular bacterial target).
    Type: Grant
    Filed: October 20, 2009
    Date of Patent: December 5, 2017
    Assignee: Becton, Dickinson and Company
    Inventors: Mei Yang-Woytowitz, Charles Yu, Timothy Wiles
  • Patent number: 9835631
    Abstract: Provided herein is a sandwich immunoassay for detecting cross-linked PIIINP that has at least two strands of PIIINP joined together by inter-strand cross-linking each having a C-terminal neo-epitope of PIIINP that is generated by N-protease cleavage of intact type III procollagen. A biological sample having the cross-linked PIIINP is contacted with a first surface-bound monoclonal antibody and then by a second monoclonal antibody, both specifically reactive with a neoepitope in the C-terminal sequence of PIIINP, and then binding of the second monoclonal antibody is determined. Also provided is a method for evaluating the efficacy of an antagonist drug targeting lysyl oxidases via the immunoassay and a kit containing a solid support binding the first monoclonal antibody and containing the second monoclonal antibody.
    Type: Grant
    Filed: February 3, 2016
    Date of Patent: December 5, 2017
    Assignee: Nordic Bioscience A/S
    Inventors: Federica Genovese, Mette Juul Nielsen, Lise Larsen, Diana Julie Oersnes-Leeming, Morten Karsdal
  • Patent number: 9810699
    Abstract: A diagnostic method for the identification of a subject suffering from a primary non-infectious disease having an increased risk of an adverse outcome potentially being induced by the administration of an antibiotic to said subject comprising the determination of the level of Procalcitonin (PCT) or a fragment thereof or a precursor or fragment thereof having a length of at least 12 amino acid residues in a sample of a bodily fluid from said subject and the correlation of the determined level to a potential risk induced by the administration of an antibiotic, as well as a kit for performing the method and methods of treatment based thereon.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: November 7, 2017
    Assignee: B.R.A.H.M.S GMBH
    Inventors: Andreas Bergmann, Joachim Struck
  • Patent number: 9708592
    Abstract: Provided are isolated polypeptides having cellulolytic enhancing activity, and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using polypeptides.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: July 18, 2017
    Assignees: Novosymes, Inc., Novozymes A/S
    Inventors: Ye Liu, Tarana Shaghasi, Matt Sweeney
  • Patent number: 9677116
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Grant
    Filed: December 1, 2015
    Date of Patent: June 13, 2017
    Assignee: BIOMARIN PHARMACEUTICAL INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 9677117
    Abstract: Provided herein are methods for detecting and quantifying succinate in a sample. Also provided are methods for detecting and quantifying 2-oxoglutarate oxygenase enzyme and/or 2-oxoglutarate oxygenase activity in a sample and methods for screening for modulators of 2-oxoglutarate oxygenase activity.
    Type: Grant
    Filed: May 18, 2015
    Date of Patent: June 13, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Juliano Alves, Said A. Goueli, Hicham Zegzouti
  • Patent number: 9588108
    Abstract: Disclosed is an apparatus for simultaneous detection of amines and thiols.
    Type: Grant
    Filed: May 22, 2014
    Date of Patent: March 7, 2017
    Assignee: ALT BIOSCIENCE, LLC.
    Inventors: Anthony Burgess-Cassler, George David McClure, Jr.
  • Patent number: 9588118
    Abstract: Apparatuses are disclosed for performing calorimetric assays with plucked human hair using a device adapted for that purpose. This device may include a transparent reaction vessel connected via a capillary tube to a burst pack, and connected via another capillary tube to a funnel, into which a plucked human hair may be placed.
    Type: Grant
    Filed: April 7, 2014
    Date of Patent: March 7, 2017
    Assignee: Follea International
    Inventors: Andy Ofer Goren, John McCoy, Daniel Hafid
  • Patent number: 9567578
    Abstract: The present invention relates to a novel GS (glutamine synthetase) gene knock out transgenic HEK293 (human embryonic kidney 293) cell line and a production method of a target protein using the said transgenic HEK293 cell line. Particularly, the present inventors eliminated the expression of GS in the HEK293 cells in order to overcome a barrier of the cell line selection caused by the over-expression of GS, for producing a target protein by GS/MSX system, by which the efficiency of the cell line selection for the high production of a target protein would be increased and accordingly the protein production by the selected cell line would be increased, suggesting that the human originated transgenic HEK293 cell line could be efficiently used for the production of a target protein.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: February 14, 2017
    Assignee: Korea Advanced Institute of Science and Technology
    Inventors: Gyun-Min Lee, Da Young Yu, Soo Min Noh
  • Patent number: 9550806
    Abstract: Immunogens against human extended-progastrin species comprise (A) a mimetic peptide comprised of (i) the amino acid sequence of a progastrin or a N- and/or C-terminal processed species of a progastrin joined to (ii) a 7 amino-acid spacer coupled to (B) an immunogenic carrier. Illustrative of the mimetic peptide/spacer combination are a 21 amino-acid peptide (SEQ ID NO.: 1) and other, related polypeptides (SEQ ID NOs.: 2-5). Pharmaceutical compositions containing such an immunogen display improved immunological properties, including the induction of effective antibody levels shortly after the administration of an initial course of immunogen. Levels of antibody thus elicited stay elevated for several months and readily elevate to higher levels upon subsequent boosting by a single injection of immunogen.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: January 24, 2017
    Assignee: ONKOLOGIX LTD.
    Inventors: Richard Ascione, Shengmei Qi, Bin Wang
  • Patent number: 9528156
    Abstract: A method for determining a predisposition of a patient to the development of muscular diseases and/or to changed biotransformation in a treatment of the patient with atorvastatin is disclosed. The presence of at least one single nucleotide polymorphism (SNP) in the UGT1A3 gene (uridine diphosphate glucuronosyltransferase gene 1A3) and/or an increased UGT1A3 gene expression is determined in a biological sample of the patient. The disclosure further relates to oligonucleotides that can be used in the method and to diagnostic kits that use the oligonucleotides.
    Type: Grant
    Filed: February 23, 2010
    Date of Patent: December 27, 2016
    Assignee: Robert Bosch GmbH
    Inventors: Kathrin Klein, Stephan Riedmaier, Ulrich Zanger
  • Patent number: 9488631
    Abstract: A method of determining preservation environment information includes irradiating left and right eyes of a fish with ultraviolet rays, taking ultraviolet images of the fish eyes by one or more ultraviolet cameras, analyzing the ultraviolet images by a computer, determining preservation environment of the fish based on luminance of each of iris portions in the left and right fish eyes, and outputting information representing a determination result to a display.
    Type: Grant
    Filed: March 12, 2015
    Date of Patent: November 8, 2016
    Assignee: PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD.
    Inventors: Yumi Wakita, Jun Ozawa
  • Patent number: 9476881
    Abstract: The present invention is directed towards methods for measuring and assaying PAS Kinase activity. The methods are useful, for example, for detecting PASK activity in a cell, and for screening for small molecule regulators of PAS kinase activity, as well as characterizing endogenous factors and stimuli that modulate PAS kinase activity, and identifying and optimizing the activity of potential PAS kinase inhibitors.
    Type: Grant
    Filed: September 12, 2014
    Date of Patent: October 25, 2016
    Assignee: BioEnergenix LLC
    Inventors: Jared Paul Rutter, Wojciech Ireneusz Swiatek