Involving Transferase Patents (Class 435/15)
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Patent number: 11527323Abstract: Systems and methods are provided for identifying a diagnosis of a cancer condition for a somatic tumor specimen of a subject. The method receives sequencing information comprising analysis of a plurality of nucleic acids derived from the somatic tumor specimen. The method identifies a plurality of features from the sequencing information, including two or more of RNA, DNA, RNA splicing, viral, and copy number features. The method provides a first subset of features and a second subset of features from the identified plurality of features as inputs to a first classifier and a second classifier, respectively. The method generates, from two or more classifiers, two or more predictions of cancer condition based at least in part on the identified plurality of features. The method combines, at a final classifier, the two or more predictions to identify the diagnosis of the cancer condition for the somatic tumor specimen of the subject.Type: GrantFiled: May 12, 2020Date of Patent: December 13, 2022Assignee: Tempus Labs, Inc.Inventors: Jackson Michuda, Kyle Ashley Beauchamp, Joshuah Kapilivsky, Calvin McCarter, Nike Beaubier, Martin Christian Stumpe, Catherine Igartua, Joshua S K Bell, Timothy Taxter, Raphael Pelossof
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Patent number: 11466300Abstract: This Invention discloses a method for production of N-Acetyl-D-Glucosamine and/or D-Glucosamine Salt by microbial fermentation. The method is intended to manufacture N-Acetyl-D-Glucosamine and/or D-Glucosamine Salt in higher efficiency and higher yield, by increasing the effects of N-Acetyl-D-Mannosamine Kinase.Type: GrantFiled: April 14, 2017Date of Patent: October 11, 2022Inventor: Lan Sun
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Patent number: 11460474Abstract: The present disclosure is directed to a method of identifying a patient having heart failure as likely to respond to a therapy with a statin. The method is based on measuring the level of at least one marker selected from GDF-15 (Growth Differentiation Factor 15), Urea, SHBG (Sex Hormone-Binding Globulin), Uric acid, PLGF (Placental Growth Factor), IL-6 (Interleukin-6), Transferrin, a cardiac Troponin, sFlt-1 (Soluble fms-like tyrosine kinase-1), Prealbumin, Ferritin, Osteopontin, sST2 (soluble ST2), and hsCRP (high sensitivity C-reactive protein) in a sample from a patient. Further envisaged is a method of predicting the risk of a patient to suffer from death or hospitalization, wherein the patient has heart failure and undergoes a therapy with a statin. The method is also based on the measurement of the level of at least one of the aforementioned markers.Type: GrantFiled: January 25, 2019Date of Patent: October 4, 2022Assignee: Roche Diagnostics Operations, Inc.Inventors: Dirk Block, Hanspeter Brunner, Thomas Dieterle, Ursula-Henrike Wienhues-Thelen, Christian Zaugg, Andre Ziegler
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Patent number: 11307204Abstract: The present disclosure relates to methods for detecting brain tumors and assessing the recurrence of such tumors by administering a pharmaceutical composition comprising 5-aminolevulinic acid (5-ALA) and detecting the conversion of 5-ALA to protoporphyrin IX (PPIX) associated with brain-derived microparticles.Type: GrantFiled: June 7, 2019Date of Patent: April 19, 2022Assignee: PIOMA, INC.Inventor: Alan M. Ezrin
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Patent number: 11273161Abstract: Methods of diagnosing and treating autism spectrum disorders are provided.Type: GrantFiled: June 12, 2019Date of Patent: March 15, 2022Assignee: The Broad Institute, Inc.Inventors: Jon Madison, Jeffrey Cottrell
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Patent number: 11274325Abstract: Provided herein, inter alia, are methods, bacteria, nucleic acids, and polypeptides for producing sialylated oligosaccharides.Type: GrantFiled: December 14, 2018Date of Patent: March 15, 2022Assignee: Glycosyn LLCInventors: Srikrishnan Mallipeddi, Matthew Ian Heidtman, Massimo Merighi, John M. McCoy
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Patent number: 11242550Abstract: The present invention discloses a method for preparing glucosyl steviol glycosides through enzymatic catalysis under programming temperatures in high throughput, belonging to the technical field of biosynthesis of sweeteners. By using cyclodextrin glucosyltransferase from Geobacillus sp. as a catalyst, steviol glycosides as the glycosyl receptor and dextrin or oligosaccharide as the glycosyl donor, taking a calcium/barium ion salt bridge as the main stabilizer and combining with glycerol to adjust the conformation and binding domain openness of the enzyme, and utilizing transglucosylation and hydrolytic activities of amylase at variable temperatures in different stages, thereby preparing the glucosyl steviol glycosides through enzymatic catalysis under programming temperatures in high throughput. The technology of the present invention can improve the utilization rate of the enzyme, and obtain glucosyl steviol glycosides with good sweetness and good taste.Type: GrantFiled: December 5, 2018Date of Patent: February 8, 2022Assignees: Jiangnan University, DONGTAI HAORUI BIOTECHNOLOGY CO., LTD.Inventors: Yongmei Xia, Liping Zhu, Yun Fang, Xiang Liu, Haijun Wang
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Patent number: 11236061Abstract: The present specification provides a compound represented by Chemical Formula 1, a colorant composition, a resin composition, a photosensitive material, a color filter and a display device comprising the same.Type: GrantFiled: February 26, 2020Date of Patent: February 1, 2022Assignee: LG CHEM, LTD.Inventors: Jongho Park, Dami Lee, Seung Jin Yang, Jihye Jung
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Patent number: 11220703Abstract: Provided herein are analytical methods utilizing a self-limiting catalytic reaction, and compositions and kits useful therefore. In one aspect the method is used to quantify Pd, and in another, the method is a horseradish peroxidase assay.Type: GrantFiled: November 23, 2016Date of Patent: January 11, 2022Assignees: University of Pittsburgh—Of the Commonwealth System of Higher Education, Merck Sharp & Dohme Corp.Inventors: Xiaodong Bu, Junyong Jo, Kazunori Koide, Matthew Patrick Tracey, Christopher J. Welch
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Patent number: 11124843Abstract: Provided is a method for detecting a bacterium of the genus Nitrobacter, comprising: a first step of amplifying a nucleotide using a test DNA as a template and primers capable of amplifying a nucleotide sequence of consecutive 110 nucleotides or more and 157 nucleotides or less in the nucleotide sequence set forth in SEQ ID NO: 1 to obtain an amplified product; and a second step of detecting the amplified product.Type: GrantFiled: March 30, 2016Date of Patent: September 21, 2021Assignee: SUMITOMO CHEMICAL COMPANY, LIMITEDInventor: Tomokazu Mitsui
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Patent number: 11066649Abstract: The invention relates to a method for inducing human cholangiocyte differentiation of progenitor cells called hepatoblasts. More specifically, the invention relates to a method for differentiating hepatoblasts to cholangiocytes by culturing said hepatoblasts with a particular medium having interleukin-6 (IL-6) activity. The differentiation method can specifically induce cholangiocyte differentiation from hepatoblasts, and the human cholangiocytes differentiated according to the invention may be useful for drug discovery for treatment of cholangiopathies and bioengineered livers.Type: GrantFiled: March 19, 2015Date of Patent: July 20, 2021Assignees: INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM), UNIVERSITÉ PARIS-SACLAY, UNIVERSITE DE RENNESInventors: Anne Dubart Kupperschmitt, Anne Weber Benarous, Anne Corlu, Noushin Dianat
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Patent number: 11021703Abstract: This invention relates to a method of characterizing the modified base status of a transcriptome, which involves contacting a transcriptome comprising one or more modified bases with an antibody specific to the modified bases under conditions effective to bind the antibody to the modified bases; isolating, from the transcriptome, a pool of RNA transcripts to which the antibody binds; and identifying isolated RNA transcripts that are present in a higher abundance in the isolated pool relative to the transcriptome, where each of the isolated RNA transcripts that are present in a higher abundance in the isolated pool together characterize the modified base status of the transcriptome. Also disclosed are a method of diagnosis or prognosis of a disease, a method of determining the effect of a treatment on modified base levels in RNA, and a kit for characterizing the modified base status of a transcriptome.Type: GrantFiled: February 18, 2013Date of Patent: June 1, 2021Assignee: CORNELL UNIVERSITYInventors: Samie R. Jaffrey, Kate D. Meyer, Christopher E. Mason
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Patent number: 10975440Abstract: Sets of experimentally validated gene specific primer pairs are provided. Embodiments of the sets include 10 or more gene specific primer pairs of forward and reverse primers. The forward and reverse primers of each primer pair include gene specific primers that are experimentally validated as suitable for use in a multiplex amplification assay. In some instances, each of the forward and reverse primers includes an anchor domain that includes a universal primer binding site. The sets find use in a variety of different applications, including high-throughput sequencing applications.Type: GrantFiled: April 19, 2016Date of Patent: April 13, 2021Assignee: Cellecta, Inc.Inventors: Alex Chenchik, Costa Frangou, Mikhail Makhanov
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Patent number: 10883992Abstract: The invention provides kinase substrates and methods comprising their use.Type: GrantFiled: February 22, 2019Date of Patent: January 5, 2021Assignees: Regents of the University of Minnesota, Yale UniversityInventors: Laurie L. Parker, Wei Cui, Benjamin Turk
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Patent number: 10870894Abstract: Methods for detecting microorganisms, in particular detection of bacteria and methods for measuring enzyme activity, such as Deoxyribonucleic acid (DNA) polymerase activity are disclosed. The aforesaid methods include, but are not limited to such methods performed on microbial crude lysates, useful for determining microbial enzyme activities, which can be linked to amplification signal generators such as real-time Polymerase Chain Reaction (PCR) techniques, thereby enabling determination of microbial pathogens in samples such as unpurified blood and other body fluids. Moreover, the disclosed embodiments also relate to reagents for use in such methods, and to test kits comprising such reagents for carrying out the methods.Type: GrantFiled: June 12, 2015Date of Patent: December 22, 2020Inventors: Shawn Mark O'Hara, Daniel Zweitzig
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Patent number: 10865392Abstract: The present invention relates to acetyl transferases, nucleic acid sequences coding therefore, expression constructs and vectors comprising these sequences, microorganisms transformed therewith and use thereof.Type: GrantFiled: October 4, 2019Date of Patent: December 15, 2020Assignee: DSM IP ASSETS B.V.Inventors: Christopher Farrell, Peter Houston, Lisa Laprade, Nathalie Balch, Maria Mayorga
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Patent number: 10813931Abstract: The compositions and methods described herein relate to the treatment of cancer, e.g. by reducing the regression of cancer cells from regressing into cancer stem cell-like phenotypes and/or reducing the development of drug-resistant cancer cells. In some embodiments, the compositions and methods relate to inhibitors of PI3K pathway kinases and Src family kinases.Type: GrantFiled: February 12, 2014Date of Patent: October 27, 2020Assignee: THE BRIGHAM AND WOMEN'S HOSPITAL, INC.Inventors: Aaron Goldman, Ashish Kulkarni, Shiladitya Sengupta
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Patent number: 10793894Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.Type: GrantFiled: February 20, 2019Date of Patent: October 6, 2020Assignee: BIOMARIN PHARMACEUTICAL INC.Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
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Patent number: 10731200Abstract: A method of quantifying ammonia, which method includes: performing a first reaction in which a test liquid containing ammonia is reacted with ATP and L-glutamic acid in the presence of glutamine synthetase to produce ADP; performing a second reaction in which the produced ADP is reacted with glucose in the presence of ADP-dependent hexokinase to produce glucose-6-phosphate; performing a third reaction in which the produced glucose-6-phosphate is reacted with an oxidized NAD compound in the presence of glucose-6-phosphate dehydrogenase to produce a reduced NAD compound; and quantifying the reduced NAD compound to quantify ammonia.Type: GrantFiled: October 19, 2017Date of Patent: August 4, 2020Assignee: ARKRAY, Inc.Inventors: Takehiro Funamoto, Masaki Murakami
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Patent number: 10724015Abstract: The present invention relates to a mutant microorganism in which a gene that encodes phosphofructokinase-2 is disrupted or deleted to reduce glycolytic flux to thereby improve the ability of the microorganism to produce N-acetylglucosamine, and to a method of producing N-acetylglucosamine using the mutant microorganism. The mutant microorganism according to the present invention has advantages in that it has high resistance to various chemical substances, grows rapidly, is easily cultured, and produces N-acetylglucosamine with high efficiency, indicating that it is useful for production of a large amount of N-acetylglucosamine.Type: GrantFiled: May 17, 2017Date of Patent: July 28, 2020Assignee: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATIONInventors: Min-Kyu Oh, Sang-Woo Lee
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Patent number: 10697870Abstract: An object is to provide a blood test kit and a blood analysis method capable of performing a blood test with a small amount of blood at high accuracy by visualizing a volume of blood collection and keeping the volume constant. Provided is a blood test kit for analyzing a concentration of a target component in a blood sample using a normal component homeostatically present in blood, the kit including: a blood collection instrument for collecting the blood sample; a diluent solution for diluting the collected blood sample; and a storing instrument for storing the diluted blood sample, in which the blood collection instrument is a capillary.Type: GrantFiled: January 3, 2018Date of Patent: June 30, 2020Assignee: FUJIFILM CorporationInventors: Hikaru Urano, Tatsuya Ishizaka, Haruyasu Nakatsugawa, Susumu Osawa, Shinya Sugimoto
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Patent number: 10688153Abstract: Modified peptides based on C-terminal PDZ binding domains of PTEN and PHLPP, or PDK1 interacting fragment of PKN2 are described as are methods of using the modified peptides for blocking the activity of PTEN, PHLPP and PKN2 and treating sudden cardiac arrest. A method for guiding treatment of cardiac arrest based on sorbitol or taurine levels is also provided.Type: GrantFiled: November 7, 2016Date of Patent: June 23, 2020Assignee: The Board of Trustees of the University of IllinoisInventors: Terry Vanden Hoek, Xiangdong Zhu, Jing Li
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Patent number: 10677786Abstract: Provided herein are methods of diagnosing or monitoring the treatment of mucopolysaccharidosis (MPS) I or a disorder associated with MPS I.Type: GrantFiled: February 8, 2018Date of Patent: June 9, 2020Assignee: BIOMARIN PHARMACEUTICAL INC.Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
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Patent number: 10632150Abstract: Disclosed herein is a sterile composition for injection comprised of potassium phosphates having no visible particulate. Also disclosed herein is a manufacturing process for the sterile composition and its use.Type: GrantFiled: April 19, 2019Date of Patent: April 28, 2020Assignee: CMP Development LLCInventors: H. Greg Thomas, Richard LeVasseur, Jeffrey S. Kiel, Anthony Reid Pipho
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Patent number: 10619186Abstract: The disclosure provides for methods, compositions, and kits for normalizing nucleic acid libraries, for example sequencing libraries.Type: GrantFiled: September 8, 2016Date of Patent: April 14, 2020Assignee: Cellular Research, Inc.Inventors: Craig Betts, Glenn Fu
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Patent number: 10596209Abstract: The present invention relates to the identification of a group of microorganisms, which are relatively abundant in the microbial communities associated with fruits and vegetables typically consumed raw and therefore transient or permanent members of the human microbiota. The consumption of mixtures of these microbes at relevant doses will produce a beneficial effect in the host by reducing the propensity to diabetes, obesity and metabolic syndrome mediated in part by production of short chain fatty acids to enhance colonic butyrate production. Therapeutic methods of the invention involve the use of live microorganisms or metabolites derived from said microorganisms to establish a microbial composition in the mammalian host that will improve significantly the ability to control weight, reduce the onset of diabetes, obesity and metabolic syndrome, and improve overall health.Type: GrantFiled: December 28, 2018Date of Patent: March 24, 2020Assignee: Solarea Bio, Inc.Inventors: Gerardo V. Toledo, Tracy Mincer, Jahir Mauricio Gutierrez Bugarin, Jillian DeWalt, Eric Schott, Maria Juliana Soto Giron
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Patent number: 10590168Abstract: The invention provides kinase substrates and methods comprising their use.Type: GrantFiled: May 23, 2017Date of Patent: March 17, 2020Assignee: REGENTS OF THE UNIVERSITY OF MINNESOTAInventors: Laurie L. Parker, Minervo Perez
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Patent number: 10585270Abstract: A multi-plane imaging system for imaging multiple reflection planes of a regular or irregular shaped three-dimensional (3D) specimen having top, side and bottom views simultaneously. The system includes an inverted watertight pyramid well having at least four reflective side surfaces for reflecting the specimen side views, wherein each of the at least four reflective sides surfaces define an angle, ?, relative to the base horizontal plane, and wherein each reflective side surface comprises a plurality of reflective zones; A specimen is positioned horizontally equidistant from the at least four reflective side surfaces and positioned vertically from the base horizontal plane a predetermined vertical distance; wherein the predetermined vertical distance, the at least four reflective side surfaces, and the angle ? comprise a reflection image plane for reflecting the specimen bottom views.Type: GrantFiled: October 25, 2018Date of Patent: March 10, 2020Assignee: University of Vermont and State Agricultural CollegeInventors: Nathan R Tykocki, Grant Hennig
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Patent number: 10570176Abstract: The present invention relates to the field of polypeptide medicine, and relates to polypeptides against hepatitis B virus X Protein and uses thereof. Specifically, the present invention relates to polypeptides comprising D-amino acids, which have a function of inhibiting hepatitis B virus X protein, and inhibit HBx activities, inhibit replication of hepatitis B virus DNA and expression of related antigens (e.g., HBeAg) at molecular level, cellular level and animal level, and further inhibit hepatitis and hepatic cirrhosis caused by hepatitis B virus infection and liver cancer that occurs on the basis of hepatic cirrhosis. The polypeptides can be widely used in the prevention and treatment of liver diseases after hepatitis B infection, including hepatitis, hepatic cirrhosis, and liver cancer.Type: GrantFiled: October 16, 2015Date of Patent: February 25, 2020Assignee: Tianjin TOPTECH Bio-Science & Technology Co., Ltd.Inventors: Xiaodong Zhang, Lihong Ye
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Patent number: 10548948Abstract: Antifungal pharmaceutical compositions, dosage forms, and methods of use thereof are disclosed.Type: GrantFiled: February 27, 2018Date of Patent: February 4, 2020Assignee: The United States of America as represented by the Secretary of the NavyInventors: Nancy Millenbaugh, Jeremy Wesley Gleaton, Dickson Kiprono Kirui
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Patent number: 10475942Abstract: A photodetector cell may include a substrate, and a first contact carried by the substrate and having a first work function value. The photodetector cell may include a second contact carried by the substrate and having a second work function value different from the first work function value, and a semiconductor wire carried by the substrate and having a third work function value between the first and second work function values. The semiconductor wire may be coupled between the first and second contacts and comprising a photodiode junction.Type: GrantFiled: May 14, 2018Date of Patent: November 12, 2019Inventors: Manjeri P. Anantram, Md Golam Rabbani, Mahmoud M. Khader, Reza Nekovei, Amit Verma
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Patent number: 10453554Abstract: The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods.Type: GrantFiled: November 7, 2014Date of Patent: October 22, 2019Assignee: Codexis Mayflower Holdings, Inc.Inventor: Richard John Fox
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Patent number: 10449182Abstract: The present invention provides sorafenib analogs for use in a method of treating a disease mediated by p21, said method comprising administering to a subject in need thereof a therapeutically effective amount of a compound of formula I. The present invention also provides methods of inhibiting p21 in a cell comprising contacting the cell with an effective amount of a compound of formula I.Type: GrantFiled: June 20, 2013Date of Patent: October 22, 2019Assignee: The Regents of the University of CaliforniaInventors: Robert H. Weiss, Bruce D. Hammock, Hiromi Inoue Wettersten, Sung Hee Hwang
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Patent number: 10422006Abstract: Phosphatidylinositol 3-kinases (PI3Ks) are known to be important regulators of signaling pathways. To determine whether PI3Ks are genetically altered in cancers, we analyzed the sequences of the PI3K gene family and discovered that one family member, PIK3CA, is frequently mutated in cancers of the colon and other organs. The majority of mutations clustered near two positions within the PI3K helical or kinase domains. PIK3CA represents one of the most highly mutated oncogenes yet identified in human cancers and is useful as a diagnostic and therapeutic target.Type: GrantFiled: October 31, 2014Date of Patent: September 24, 2019Assignee: The John Hopkins UniversityInventors: Yardena Samuels, Victor Velculescu, Kenneth W. Kinzler, Bert Vogelstein
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Patent number: 10405810Abstract: A tool for predicting that a person is likely to be abnormally hydrated over a future time interval, and in some cases to alert the person or a medical professional to intervene. From a series of physiological measurements, a tissue electrical impedance spectrum curve, which comprises a phase spectrum curve in an embodiment, is determined and changes in the shape of the curve are ascertained. The measurements may be received using one or more sensors worn by the person. In some embodiments, current and historic spectrum curvature are applied to an evolutionary algorithm, such as particle-swarm optimization (PSO) or differential evolution (DE), to determine an inference regarding the persons future hydration status. In one embodiment, a statistical forecast for the next epoch immediately beyond the present one, is determined.Type: GrantFiled: May 23, 2016Date of Patent: September 10, 2019Assignee: Cerner Innovation, Inc.Inventor: Douglas S. McNair
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Patent number: 10407735Abstract: The present invention relates to compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present invention relates to ncRNAs as diagnostic markers and clinical targets for prostate, lung, breast and pancreatic cancer.Type: GrantFiled: May 17, 2016Date of Patent: September 10, 2019Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: Arul Chinnaiyan, John Prensner, Matthew Iyer
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Patent number: 10364425Abstract: The invention is related to a composition of recombinant or transgenic Factor VII, each molecule of Factor VII of the composition exhibiting two N-glycosylation sites, wherein, among all the molecules of FVII of the composition, the rate of Gal?1,3G al glycan moieties is comprised between 0 and 4%. The invention is also related to a process for preparing such a composition of FVII.Type: GrantFiled: May 2, 2013Date of Patent: July 30, 2019Assignee: LABORATOIRE FRANCAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIESInventors: Abdessatar Sami Chtourou, Emmanuel Nony, Nicolas Bihoreau
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Patent number: 10316349Abstract: The present invention is a method for measuring the amount of at least one molecule in a biological sample, the method comprising a) combining the sample, or a derivative thereof, with one or more aptamers and allowing one or more molecules in the sample to bind to the aptamer(s); b) separating bound from unbound molecules; and c) quantifying the molecule(s) bound to the or each aptamer, wherein quantification of the bound molecule(s) is carried out by sequencing at least part of the or each aptamer. Uses of and products derived from the method are also contemplated.Type: GrantFiled: August 4, 2017Date of Patent: June 11, 2019Assignee: Caris Science, Inc.Inventors: Clive Gavin Brown, Koen Kas, Sven Agnes Jan Eyckerman
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Patent number: 10294240Abstract: [Problem] To provide a fluorescent probe that detects calpain activity in cells at high sensitivity. [Solution] A compound represented by the following general formula (I) or a salt thereof.Type: GrantFiled: February 29, 2016Date of Patent: May 21, 2019Assignees: The University of Tokyo, Tohoku UniversityInventors: Yasuteru Urano, Yuri Nagayo, Mako Kamiya, Toru Nakazawa
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Patent number: 10287590Abstract: The invention provides methods for introducing co-varying paired nucleic acids into a vector such under separate transcriptional control. An oligonucleotide is synthesized including the paired nucleic acids. The oligonucleotide is then assembled with a spacer nucleic acid encoding a promoter. After assembly the spacer nucleic acid is to one side of the oligonucleotide encoding the paired segments. However, on circularization of the assembled nucleic acid and cleavage between the DNA segments the spacer oligonucleotide and its components now occur between the paired segments. The resulting nucleic acid can now be cloned into a vector with a single step, such that each of the paired nucleic acid segments is linked to its own promoter. The present method can readily be extended to library screening without proportionately increasing the effort.Type: GrantFiled: December 24, 2014Date of Patent: May 14, 2019Assignee: DNA2.0, INC.Inventors: Jeremy Minshull, Sridhar Govindrajan, Kedar Patel
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Patent number: 10287571Abstract: The present invention relates to a modified microorganism having, compared to its wildtype, —a reduced activity of an enzyme encoded by the ptsA-gene, —a reduced activity of an enzyme encoded by the ptsH-gene or —a reduced activity of an enzyme encoded by the ptsA-gene and a reduced activity of an enzyme encoded by the ptsH-gene, wherein the wildtype from which the modified microorganism has been derived belongs to the family of Pasteurellaceae. The present invention also relates to a method for producing succinic acid and to the use of modified microorganisms.Type: GrantFiled: May 7, 2015Date of Patent: May 14, 2019Assignee: BASF SEInventors: Joanna Martyna Krawczyk, Stefan Haefner, Hartwig Schroeder, Oskar Zelder
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Patent number: 10260084Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.Type: GrantFiled: May 12, 2017Date of Patent: April 16, 2019Assignee: BIOMARIN PHARMACEUTICAL INC.Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
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Patent number: 10233439Abstract: The present invention discloses a directionally modified glucosamine synthase mutant and its application. The amino acid sequence of the glucosamine synthase mutant is as shown in sequence list SEQ ID No. 1, and the nucleotide sequence is as shown in sequence list SEQ ID No. 2. The genetic engineering bacteria of glucosamine synthase is successfully constructed. In order to improve the tolerance of recombinant bacteria against glucosamine, the glucosamine synthase is directionally modified. A glucosamine synthase mutant is selected from the mutant library via high-throughput screening method, the amino acid changes in the mutant induces the spatial conformational change in the enzyme, so as enlarged the region where the enzyme and substrate combines, therefor the combination efficiency of the enzyme and the substrate is increased. The glucosamine synthase of the present invention has various advantages, such as rich in raw material of glucose, and a convenient subsequent extraction.Type: GrantFiled: June 5, 2017Date of Patent: March 19, 2019Assignees: Yangzhou Rixing Bio-Tech Co., Ltd, Jiangnan UniversityInventors: Jinsong Shi, Zhenghong Xu, Chao Zhang, Jinsong Gong, Heng Li, Zhenzhong Ding, Xiang Fang, Wanhong Zhang
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Patent number: 10196692Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.Type: GrantFiled: September 18, 2015Date of Patent: February 5, 2019Assignee: QIAGEN MANCHESTER LIMITEDInventors: Ruth Board, Jennifer Haworth, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
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Patent number: 10190171Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.Type: GrantFiled: September 18, 2015Date of Patent: January 29, 2019Assignee: QIAGEN MANCHESTER LIMITEDInventors: Ruth Board, Jennifer Haworth, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
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Patent number: 10175226Abstract: A method for quantifying acetylamantadine in a urine sample comprises eluting acetylamantadine from the urine sample using solid phase extraction and quantifying the acetylamantadine eluted from the urine sample using Raman spectroscopy.Type: GrantFiled: July 10, 2017Date of Patent: January 8, 2019Assignee: BioMark Technologies, Inc.Inventors: Reuven Gordon, Brian Cheng, Rashid Bux, Bram Ramjiawan, Aftab Ahmed, Fraser Alan Hof
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Patent number: 10077244Abstract: A method to detect the presence of amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.Type: GrantFiled: November 25, 2014Date of Patent: September 18, 2018Assignee: PROMEGA CORPORATIONInventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
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Patent number: 10059955Abstract: The invention relates to isolated nucleic acids encoding a feruloyl-CoA:monolignol transferase and feruloyl-CoA:monolignol transferase enzymes. The isolated nucleic acids and/or the enzymes enable incorporation of monolignol ferulates into the lignin of plants, where such monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate. The invention also includes methods and plants that include nucleic acids encoding a feruloyl-CoA:monolignol transferase enzyme and/or feruloyl-CoA:monolignol transferase enzymes.Type: GrantFiled: October 4, 2016Date of Patent: August 28, 2018Assignees: Board of Trustees of Michigan State University, The University of British Columbia, Wisconsin Alumni Resarch FoundationInventors: Curtis Wilkerson, John Ralph, Saunia Withers, Shawn D. Mansfield
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Patent number: 10035974Abstract: The objective of the present invention is to provide a cleaning agent composition which can be used in the open air without any problems since the cleaning agent is environmentally friendly and which is effective for removing a low-molecular-weight and poorly-water-soluble cyclic silicone compound that causes a stain of a silicone sealing. The cleaning agent for washing out a silicone stain according to the present invention is characterized in comprising a cyclic lipopeptide biosurfactant, wherein the specific silicone stain compound such as D4 compound is the objective compound to be removed.Type: GrantFiled: March 25, 2014Date of Patent: July 31, 2018Assignees: KANEKA CORPORATION, NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Koji Noda, Masashi Izumida, Satohiro Yanagisawa, Tohru Nakashima, Takuto Nagano, Toshiaki Taira, Tomohiro Imura, Dai Kitamoto
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Patent number: RE49518Abstract: [Problem] To provide a fluorescent probe that detects calpain activity in cells at high sensitivity. [Solution] A compound represented by the following general formula (I) or a salt thereof.Type: GrantFiled: May 21, 2021Date of Patent: May 2, 2023Assignees: The University of Tokyo, Tohoku UniversityInventors: Yasuteru Urano, Yuri Nagayo, Mako Kamiya, Toru Nakazawa