Involving Transferase Patents (Class 435/15)
  • Patent number: 10316349
    Abstract: The present invention is a method for measuring the amount of at least one molecule in a biological sample, the method comprising a) combining the sample, or a derivative thereof, with one or more aptamers and allowing one or more molecules in the sample to bind to the aptamer(s); b) separating bound from unbound molecules; and c) quantifying the molecule(s) bound to the or each aptamer, wherein quantification of the bound molecule(s) is carried out by sequencing at least part of the or each aptamer. Uses of and products derived from the method are also contemplated.
    Type: Grant
    Filed: August 4, 2017
    Date of Patent: June 11, 2019
    Assignee: Caris Science, Inc.
    Inventors: Clive Gavin Brown, Koen Kas, Sven Agnes Jan Eyckerman
  • Patent number: 10294240
    Abstract: [Problem] To provide a fluorescent probe that detects calpain activity in cells at high sensitivity. [Solution] A compound represented by the following general formula (I) or a salt thereof.
    Type: Grant
    Filed: February 29, 2016
    Date of Patent: May 21, 2019
    Assignees: The University of Tokyo, Tohoku University
    Inventors: Yasuteru Urano, Yuri Nagayo, Mako Kamiya, Toru Nakazawa
  • Patent number: 10287590
    Abstract: The invention provides methods for introducing co-varying paired nucleic acids into a vector such under separate transcriptional control. An oligonucleotide is synthesized including the paired nucleic acids. The oligonucleotide is then assembled with a spacer nucleic acid encoding a promoter. After assembly the spacer nucleic acid is to one side of the oligonucleotide encoding the paired segments. However, on circularization of the assembled nucleic acid and cleavage between the DNA segments the spacer oligonucleotide and its components now occur between the paired segments. The resulting nucleic acid can now be cloned into a vector with a single step, such that each of the paired nucleic acid segments is linked to its own promoter. The present method can readily be extended to library screening without proportionately increasing the effort.
    Type: Grant
    Filed: December 24, 2014
    Date of Patent: May 14, 2019
    Assignee: DNA2.0, INC.
    Inventors: Jeremy Minshull, Sridhar Govindrajan, Kedar Patel
  • Patent number: 10287571
    Abstract: The present invention relates to a modified microorganism having, compared to its wildtype, —a reduced activity of an enzyme encoded by the ptsA-gene, —a reduced activity of an enzyme encoded by the ptsH-gene or —a reduced activity of an enzyme encoded by the ptsA-gene and a reduced activity of an enzyme encoded by the ptsH-gene, wherein the wildtype from which the modified microorganism has been derived belongs to the family of Pasteurellaceae. The present invention also relates to a method for producing succinic acid and to the use of modified microorganisms.
    Type: Grant
    Filed: May 7, 2015
    Date of Patent: May 14, 2019
    Assignee: BASF SE
    Inventors: Joanna Martyna Krawczyk, Stefan Haefner, Hartwig Schroeder, Oskar Zelder
  • Patent number: 10260084
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: April 16, 2019
    Assignee: BIOMARIN PHARMACEUTICAL INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 10233439
    Abstract: The present invention discloses a directionally modified glucosamine synthase mutant and its application. The amino acid sequence of the glucosamine synthase mutant is as shown in sequence list SEQ ID No. 1, and the nucleotide sequence is as shown in sequence list SEQ ID No. 2. The genetic engineering bacteria of glucosamine synthase is successfully constructed. In order to improve the tolerance of recombinant bacteria against glucosamine, the glucosamine synthase is directionally modified. A glucosamine synthase mutant is selected from the mutant library via high-throughput screening method, the amino acid changes in the mutant induces the spatial conformational change in the enzyme, so as enlarged the region where the enzyme and substrate combines, therefor the combination efficiency of the enzyme and the substrate is increased. The glucosamine synthase of the present invention has various advantages, such as rich in raw material of glucose, and a convenient subsequent extraction.
    Type: Grant
    Filed: June 5, 2017
    Date of Patent: March 19, 2019
    Assignees: Yangzhou Rixing Bio-Tech Co., Ltd, Jiangnan University
    Inventors: Jinsong Shi, Zhenghong Xu, Chao Zhang, Jinsong Gong, Heng Li, Zhenzhong Ding, Xiang Fang, Wanhong Zhang
  • Patent number: 10196692
    Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.
    Type: Grant
    Filed: September 18, 2015
    Date of Patent: February 5, 2019
    Assignee: QIAGEN MANCHESTER LIMITED
    Inventors: Ruth Board, Jennifer Haworth, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
  • Patent number: 10190171
    Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.
    Type: Grant
    Filed: September 18, 2015
    Date of Patent: January 29, 2019
    Assignee: QIAGEN MANCHESTER LIMITED
    Inventors: Ruth Board, Jennifer Haworth, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
  • Patent number: 10175226
    Abstract: A method for quantifying acetylamantadine in a urine sample comprises eluting acetylamantadine from the urine sample using solid phase extraction and quantifying the acetylamantadine eluted from the urine sample using Raman spectroscopy.
    Type: Grant
    Filed: July 10, 2017
    Date of Patent: January 8, 2019
    Assignee: BioMark Technologies, Inc.
    Inventors: Reuven Gordon, Brian Cheng, Rashid Bux, Bram Ramjiawan, Aftab Ahmed, Fraser Alan Hof
  • Patent number: 10077244
    Abstract: A method to detect the presence of amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: September 18, 2018
    Assignee: PROMEGA CORPORATION
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • Patent number: 10059955
    Abstract: The invention relates to isolated nucleic acids encoding a feruloyl-CoA:monolignol transferase and feruloyl-CoA:monolignol transferase enzymes. The isolated nucleic acids and/or the enzymes enable incorporation of monolignol ferulates into the lignin of plants, where such monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate. The invention also includes methods and plants that include nucleic acids encoding a feruloyl-CoA:monolignol transferase enzyme and/or feruloyl-CoA:monolignol transferase enzymes.
    Type: Grant
    Filed: October 4, 2016
    Date of Patent: August 28, 2018
    Assignees: Board of Trustees of Michigan State University, The University of British Columbia, Wisconsin Alumni Resarch Foundation
    Inventors: Curtis Wilkerson, John Ralph, Saunia Withers, Shawn D. Mansfield
  • Patent number: 10035974
    Abstract: The objective of the present invention is to provide a cleaning agent composition which can be used in the open air without any problems since the cleaning agent is environmentally friendly and which is effective for removing a low-molecular-weight and poorly-water-soluble cyclic silicone compound that causes a stain of a silicone sealing. The cleaning agent for washing out a silicone stain according to the present invention is characterized in comprising a cyclic lipopeptide biosurfactant, wherein the specific silicone stain compound such as D4 compound is the objective compound to be removed.
    Type: Grant
    Filed: March 25, 2014
    Date of Patent: July 31, 2018
    Assignees: KANEKA CORPORATION, NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Koji Noda, Masashi Izumida, Satohiro Yanagisawa, Tohru Nakashima, Takuto Nagano, Toshiaki Taira, Tomohiro Imura, Dai Kitamoto
  • Patent number: 9997656
    Abstract: A photodetector cell may include a substrate, and a first contact carried by the substrate and having a first work function value. The photodetector cell may include a second contact carried by the substrate and having a second work function value different from the first work function value, and a semiconductor wire carried by the substrate and having a third work function value between the first and second work function values. The semiconductor wire may be coupled between the first and second contacts and comprising a photodiode junction.
    Type: Grant
    Filed: December 17, 2015
    Date of Patent: June 12, 2018
    Inventors: Manjeri P. Anantram, Md Golam Rabbani, Mahmoud M. Khader, Reza Nekovei, Amit Verma
  • Patent number: 9982242
    Abstract: Provided are: a protein complex capable of selectively and asymmetrically oxidizing an enantiomer of a secondary alcohol without adding a coenzyme and having an asymmetric oxidation activity in a water-soluble solvent system in the presence of oxygen; a method for producing the same; and a method for coating the protein complex with a high molecular weight compound. The method for producing the protein complex includes: (1) enclosing a crude water-soluble protein in a gel, air-oxidizing the gel, and eluting the protein complex into an aqueous solution; and (2) applying gravity to concentrate and precipitate the protein complex, redissolving the precipitate in an aqueous glycine sodium hydroxide solution of about 0.5 mM and allowing the same to homogeneously coexist with a high molecular weight compound, and re-precipitating the solution and dehydrating and drying the same to yield a protein complex coated with a high molecular weight compound.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: May 29, 2018
    Assignee: SANYO FOODS CO., LTD.
    Inventor: Hiroyuki Nagaoka
  • Patent number: 9915649
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Grant
    Filed: April 12, 2016
    Date of Patent: March 13, 2018
    Assignee: BIOMARIN PHARMACEUTICALS INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 9896666
    Abstract: The present invention relates to a method for stabilizing an enzyme by storing the enzyme in the presence of a stable coenzyme. The present invention further relates to an enzyme stabilized with a stable coenzyme, and to the use thereof in test elements for detecting analytes.
    Type: Grant
    Filed: August 19, 2010
    Date of Patent: February 20, 2018
    Assignee: ROCHE DIABETES CARE, INC.
    Inventors: Carina Horn, Dieter Heindl, Claudia Gaessler-Dietsche, Joachim Hoenes
  • Patent number: 9879304
    Abstract: The present invention provides a method of quantifying the activity of a protein modifying enzyme in a sample, comprising: (i) grouping modified peptides from a first sample and modified peptides from a second sample into a single group according to one of the following parameters: (a) modified peptides having a modification site that is modified by the same protein modifying enzyme; or (b) modified peptides having a modification site that is part of the same modification motif; (ii) calculating enrichment of the modified peptides from the first sample compared to the modified peptides from the second sample in the group; and (iii) calculating the statistical significance of said enrichment; wherein a statistically significant enrichment is indicative of a protein modifying enzyme being activated in the first sample compared to the second sample. In some embodiments, the method further comprises identifying modified peptides in a first sample and a second sample using mass spectrometry (MS) prior to step (i).
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: January 30, 2018
    Assignee: Queen Mary & Westfield College, University of London
    Inventor: Pedro Rodriguez Cutillas
  • Patent number: 9874567
    Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enzymes. Kits may include an element tag for directly tagging an affinity product that recognizes phosphorylated substrates, as well as a non-phosphorylated substrate or an expression plasmid.
    Type: Grant
    Filed: February 2, 2016
    Date of Patent: January 23, 2018
    Assignee: Fluidigm Canada Inc.
    Inventor: Olga Ornatsky
  • Patent number: 9834807
    Abstract: Presented herein are solid supports on or in which a composition comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent) is present in added form. Presented herein are also methods for detecting the presence of an intracellular microorganism target (e.g., an intracellular bacterial target) utilizing such solid supports. Further, presented herein are tablets as well as dry powders comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent), and methods of using such tablets and dry powders to detect the presence of an intracellular microorganism target (e.g., an intracellular bacterial target).
    Type: Grant
    Filed: October 20, 2009
    Date of Patent: December 5, 2017
    Assignee: Becton, Dickinson and Company
    Inventors: Mei Yang-Woytowitz, Charles Yu, Timothy Wiles
  • Patent number: 9835631
    Abstract: Provided herein is a sandwich immunoassay for detecting cross-linked PIIINP that has at least two strands of PIIINP joined together by inter-strand cross-linking each having a C-terminal neo-epitope of PIIINP that is generated by N-protease cleavage of intact type III procollagen. A biological sample having the cross-linked PIIINP is contacted with a first surface-bound monoclonal antibody and then by a second monoclonal antibody, both specifically reactive with a neoepitope in the C-terminal sequence of PIIINP, and then binding of the second monoclonal antibody is determined. Also provided is a method for evaluating the efficacy of an antagonist drug targeting lysyl oxidases via the immunoassay and a kit containing a solid support binding the first monoclonal antibody and containing the second monoclonal antibody.
    Type: Grant
    Filed: February 3, 2016
    Date of Patent: December 5, 2017
    Assignee: Nordic Bioscience A/S
    Inventors: Federica Genovese, Mette Juul Nielsen, Lise Larsen, Diana Julie Oersnes-Leeming, Morten Karsdal
  • Patent number: 9810699
    Abstract: A diagnostic method for the identification of a subject suffering from a primary non-infectious disease having an increased risk of an adverse outcome potentially being induced by the administration of an antibiotic to said subject comprising the determination of the level of Procalcitonin (PCT) or a fragment thereof or a precursor or fragment thereof having a length of at least 12 amino acid residues in a sample of a bodily fluid from said subject and the correlation of the determined level to a potential risk induced by the administration of an antibiotic, as well as a kit for performing the method and methods of treatment based thereon.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: November 7, 2017
    Assignee: B.R.A.H.M.S GMBH
    Inventors: Andreas Bergmann, Joachim Struck
  • Patent number: 9708592
    Abstract: Provided are isolated polypeptides having cellulolytic enhancing activity, and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using polypeptides.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: July 18, 2017
    Assignees: Novosymes, Inc., Novozymes A/S
    Inventors: Ye Liu, Tarana Shaghasi, Matt Sweeney
  • Patent number: 9677116
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Grant
    Filed: December 1, 2015
    Date of Patent: June 13, 2017
    Assignee: BIOMARIN PHARMACEUTICAL INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 9677117
    Abstract: Provided herein are methods for detecting and quantifying succinate in a sample. Also provided are methods for detecting and quantifying 2-oxoglutarate oxygenase enzyme and/or 2-oxoglutarate oxygenase activity in a sample and methods for screening for modulators of 2-oxoglutarate oxygenase activity.
    Type: Grant
    Filed: May 18, 2015
    Date of Patent: June 13, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Juliano Alves, Said A. Goueli, Hicham Zegzouti
  • Patent number: 9588108
    Abstract: Disclosed is an apparatus for simultaneous detection of amines and thiols.
    Type: Grant
    Filed: May 22, 2014
    Date of Patent: March 7, 2017
    Assignee: ALT BIOSCIENCE, LLC.
    Inventors: Anthony Burgess-Cassler, George David McClure, Jr.
  • Patent number: 9588118
    Abstract: Apparatuses are disclosed for performing calorimetric assays with plucked human hair using a device adapted for that purpose. This device may include a transparent reaction vessel connected via a capillary tube to a burst pack, and connected via another capillary tube to a funnel, into which a plucked human hair may be placed.
    Type: Grant
    Filed: April 7, 2014
    Date of Patent: March 7, 2017
    Assignee: Follea International
    Inventors: Andy Ofer Goren, John McCoy, Daniel Hafid
  • Patent number: 9567578
    Abstract: The present invention relates to a novel GS (glutamine synthetase) gene knock out transgenic HEK293 (human embryonic kidney 293) cell line and a production method of a target protein using the said transgenic HEK293 cell line. Particularly, the present inventors eliminated the expression of GS in the HEK293 cells in order to overcome a barrier of the cell line selection caused by the over-expression of GS, for producing a target protein by GS/MSX system, by which the efficiency of the cell line selection for the high production of a target protein would be increased and accordingly the protein production by the selected cell line would be increased, suggesting that the human originated transgenic HEK293 cell line could be efficiently used for the production of a target protein.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: February 14, 2017
    Assignee: Korea Advanced Institute of Science and Technology
    Inventors: Gyun-Min Lee, Da Young Yu, Soo Min Noh
  • Patent number: 9550806
    Abstract: Immunogens against human extended-progastrin species comprise (A) a mimetic peptide comprised of (i) the amino acid sequence of a progastrin or a N- and/or C-terminal processed species of a progastrin joined to (ii) a 7 amino-acid spacer coupled to (B) an immunogenic carrier. Illustrative of the mimetic peptide/spacer combination are a 21 amino-acid peptide (SEQ ID NO.: 1) and other, related polypeptides (SEQ ID NOs.: 2-5). Pharmaceutical compositions containing such an immunogen display improved immunological properties, including the induction of effective antibody levels shortly after the administration of an initial course of immunogen. Levels of antibody thus elicited stay elevated for several months and readily elevate to higher levels upon subsequent boosting by a single injection of immunogen.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: January 24, 2017
    Assignee: ONKOLOGIX LTD.
    Inventors: Richard Ascione, Shengmei Qi, Bin Wang
  • Patent number: 9528156
    Abstract: A method for determining a predisposition of a patient to the development of muscular diseases and/or to changed biotransformation in a treatment of the patient with atorvastatin is disclosed. The presence of at least one single nucleotide polymorphism (SNP) in the UGT1A3 gene (uridine diphosphate glucuronosyltransferase gene 1A3) and/or an increased UGT1A3 gene expression is determined in a biological sample of the patient. The disclosure further relates to oligonucleotides that can be used in the method and to diagnostic kits that use the oligonucleotides.
    Type: Grant
    Filed: February 23, 2010
    Date of Patent: December 27, 2016
    Assignee: Robert Bosch GmbH
    Inventors: Kathrin Klein, Stephan Riedmaier, Ulrich Zanger
  • Patent number: 9488631
    Abstract: A method of determining preservation environment information includes irradiating left and right eyes of a fish with ultraviolet rays, taking ultraviolet images of the fish eyes by one or more ultraviolet cameras, analyzing the ultraviolet images by a computer, determining preservation environment of the fish based on luminance of each of iris portions in the left and right fish eyes, and outputting information representing a determination result to a display.
    Type: Grant
    Filed: March 12, 2015
    Date of Patent: November 8, 2016
    Assignee: PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD.
    Inventors: Yumi Wakita, Jun Ozawa
  • Patent number: 9476881
    Abstract: The present invention is directed towards methods for measuring and assaying PAS Kinase activity. The methods are useful, for example, for detecting PASK activity in a cell, and for screening for small molecule regulators of PAS kinase activity, as well as characterizing endogenous factors and stimuli that modulate PAS kinase activity, and identifying and optimizing the activity of potential PAS kinase inhibitors.
    Type: Grant
    Filed: September 12, 2014
    Date of Patent: October 25, 2016
    Assignee: BioEnergenix LLC
    Inventors: Jared Paul Rutter, Wojciech Ireneusz Swiatek
  • Patent number: 9464122
    Abstract: Tau protein has a causative role in Alzheimer's disease and multiple other neurodegenerative disorders exhibiting tau histopathology collectively termed tauopathies. The primary function of tau protein is to facilitate assembly and maintenance of microtubules in neuronal axons. In the disease process tau protein becomes modified, loses its affinity to microtubules and accumulates in the cell body where it forms aggregates. The large neurofibrillary tangles formed from tau protein assembled into filaments were thought to be the pathological structure of tau. However, more recent work indicates that smaller, soluble oligomeric forms of tau are best associated with neuron loss and memory impairment. Here, novel compositions of tau oligomers and novel mechanisms for tau oligomer nucleation, extension and termination are taught.
    Type: Grant
    Filed: August 21, 2009
    Date of Patent: October 11, 2016
    Assignee: Oligomerix, Inc.
    Inventors: James G. Moe, Eliot J. Davidowitz
  • Patent number: 9458476
    Abstract: A method for producing glycerin from one or more plants of genus Nicotiana is provided. The glycerin can be derived inter alia from Nicotiana species biomass or from seed. In certain embodiments, the glycerin is produced by either acid-catalyzed hydrolysis or base-catalyzed hydrolysis of acylglycerol-containing starting material derived from tobacco biomass or seed. The invention also provides articles and compositions including smoking articles and smokeless tobacco compositions that include glycerin produced from one or more plants of genus Nicotiana.
    Type: Grant
    Filed: April 18, 2011
    Date of Patent: October 4, 2016
    Assignee: R.J. Reynolds Tobacco Company
    Inventors: William Coleman, Michael Francis Dube
  • Patent number: 9410183
    Abstract: Methods and kits for detecting the presence of GlcNAc modification of a peptide or protein. The steps of detection may include combining the peptide or protein with PAP35S and a sulfotransferase to produce a reaction product including 35S labeled GlcNAc modified protein or peptide, removing background labeling with a glycosidase, separating the 35S labeled GlcNAc modified protein or peptide from PAP35S, free S-35 sulfate and labeled oligosaccharides if present, and detecting isotopic emissions from the 35S labeled GlcNAc modified peptide or protein. The detection may further include, prior to detection, the step of combining the peptide or protein with a GlcNAc transferase and UDP-GlcNAc under appropriate conditions for the GlcNAc transferase to transfer a GlcNAc to the peptide or protein.
    Type: Grant
    Filed: September 25, 2014
    Date of Patent: August 9, 2016
    Assignee: Bio-Techne Corporation
    Inventor: Zhengliang L. Wu
  • Patent number: 9410182
    Abstract: A kit for detecting or measuring glycosyltransferase activity including a first reagent comprising a phosphatase and a second reagent comprising a free phosphate detector, and method of detecting and measuring glycosyltransferase activity. A sugar donor, an acceptor substrate, a glycosyltransferase enzyme and a phosphatase are combined and the amount of free phosphate present in the product is measured and used to calculate the activity of the glycosyltransferase enzyme.
    Type: Grant
    Filed: May 24, 2010
    Date of Patent: August 9, 2016
    Assignee: Bio-Techne Corporation
    Inventor: Zhengliang L. Wu
  • Patent number: 9395300
    Abstract: The invention relates to a method for the spectrophotometric determination of the concentration of multiple substances, preferably bilirubin, haemoglobin and lipids, in a sample of body fluid.
    Type: Grant
    Filed: July 16, 2012
    Date of Patent: July 19, 2016
    Assignee: SIEMENS HEALTHCARE DIAGNOSTICS PRODUCTS GMBH
    Inventors: Karl Sass, Dirk Greis, Michael Noah, Christian Ueckermann
  • Patent number: 9345764
    Abstract: The present application provides compositions and methods useful for treating and diagnosing diseases and disorders associated with moesin activation.
    Type: Grant
    Filed: October 8, 2011
    Date of Patent: May 24, 2016
    Assignee: SHANGHAI KEXIN BIOTECH CO., LTD.
    Inventors: Yue Zhang, Jun Bao, Hua Mao, Zhinan Shou, Weina Situ
  • Patent number: 9340822
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Grant
    Filed: August 18, 2014
    Date of Patent: May 17, 2016
    Assignee: BIOMARIN PHARMACEUTICAL INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 9328341
    Abstract: Methods are provided for modulating the activity of multimeric ubiquitin-protein E3 ligases including, but not limited to, E6AP ligase activities. The methods reduce the level of oligomer formation such as homotrimeric E6AP ligase to reduce the enzyme activity. Alternatively, agents are provided that can promote the association of the ligase monomers, thereby increasing the ligase activity. Accordingly, novel therapeutic strategies are provided that are useful for the treatment of pathologies resulting from mutations in the genes encoding the ligases and which adversely increase or decrease a ubiquitination reaction.
    Type: Grant
    Filed: April 14, 2015
    Date of Patent: May 3, 2016
    Assignee: Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
    Inventors: Arthur L. Haas, Virginia P. Ronchi
  • Patent number: 9285374
    Abstract: The present invention provides an ex vivo method for aiding the diagnosis of Alzheimer's disease in a patient, the method comprising the steps of determining the level of expression of at least four platelet proteins in a platelet sample from the patient selected from monoamine oxidase-B, coagulation factor Xllla, total tropomyosin (a and 13), WD-repeat protein 1 and apolipoprotein E4; and comparing the result of (i) to a control value, wherein a result higher than the control value is indicative of Alzheimer's disease. Preferably, the method of the invention further comprises determining the level of expression of wild-type GSTO-1 or mutant GSTO-1.
    Type: Grant
    Filed: December 3, 2010
    Date of Patent: March 15, 2016
    Assignee: RANDOX LABORATORIES LIMITED
    Inventors: Ellen Umlauf, Maria Zellner
  • Patent number: 9222120
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Grant
    Filed: July 1, 2014
    Date of Patent: December 29, 2015
    Assignee: BioMarin Pharmaceutical Inc.
    Inventors: Brett E. Crawford, Jilllian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 9194802
    Abstract: A molecular probe for the luminescent detection of nucleotides (e.g., adenosine nucleotides) is presented. In certain embodiments, the probe can readily distinguish between the three adenosine nucleotides in buffered aqueous conditions at neutral pH, a need for the direct monitoring of enzymatic reactions converting ATP to ADP or AMP. The probe is most efficient under millimolar concentrations of ATP, which are relevant to intracellular conditions. In preferred embodiments, the long luminescence lifetime of the probe readily enables time-gating experiments.
    Type: Grant
    Filed: September 10, 2013
    Date of Patent: November 24, 2015
    Assignee: Regents of the University of Minnesota
    Inventor: Valerie Christine Pierre
  • Patent number: 9174932
    Abstract: By searching various kinds of compounds having CaSR agonistic activity, the present invention provides CaSR agonistic agents, pharmaceutical compositions, preventive or therapeutic agents for diarrhea and kokumi-imparting agents each of which comprise the compound. More specifically, the present invention provides CaSR agonistic agents, pharmaceutical compositions, preventive or therapeutic agents for diarrhea and kokumi-imparting agents each of which comprise a glutamic acid derivative having CaSR agonistic activity or pharmaceutically acceptable salts thereof.
    Type: Grant
    Filed: March 28, 2014
    Date of Patent: November 3, 2015
    Assignee: AJINOMOTO CO., INC.
    Inventors: Masayuki Sugiki, Toru Okamatsu, Sayaka Asari, Yayoi Kawato, Toshihiro Hatanaka, Tetsuo Yano, Yukie Seki, Naohiro Miyamura, Hiroaki Nagasaki, Yuzuru Eto, Reiko Yasuda
  • Patent number: 9175043
    Abstract: Control agents for immuno-precipitation assays, methods of using the control agents and kits comprising the control agents are provided.
    Type: Grant
    Filed: March 29, 2013
    Date of Patent: November 3, 2015
    Assignee: The General Hospital Corporation
    Inventors: Bradley E. Bernstein, Alon Goren
  • Patent number: 9128099
    Abstract: Methods for determining the presence or amount of a complex comprising a first and second molecular entity are provided, preferably an sFlt-1:PlGF complex. A determination of the presence or amount of the complex can be used in methods for predicting, detecting, monitoring a disease, or guiding therapy in respect to a disease such as vascular, vascular-related disease, cardiac, cardiac-related disease, cancer, cancer-related disease, preeclampsia, and preeclampsia-related disease. Determining sFlt-1:angiogenic factor complex is particularly useful for predicting and detecting preeclampsia in early stages of gestation and in stages of the disease where clinical evaluation may be uninformative.
    Type: Grant
    Filed: September 12, 2014
    Date of Patent: September 8, 2015
    Assignee: Ortho-Clinical Diagnostics, Inc.
    Inventors: George Bashirians, Deborah Byrne, Sarah Groulx, Sharon Jackson, Heather Lane-Brown, Banumathi Sankaran, Edward Scalice, Karen Yang, Jian Zheng
  • Patent number: 9109048
    Abstract: The present invention provides a cytotoxic 7 to 25 mer peptide with three or more cationic residues which has one or more non-genetic bulky and lipophilic amino acids, as well as esters, amides, salts and cyclic derivatives thereof as well as methods of preparing the peptides, pharmaceutical compositions containing them and their use as medicaments, particularly as antibacterions or antitumoral agents. In a preferred aspect, the invention provides the use of said peptides in a method of inducing adaptive immunity against tumor growth or establishment in a subject, as well as the use of other lytic agents in a method of inducing adaptive immunity in a subject.
    Type: Grant
    Filed: August 14, 2012
    Date of Patent: August 18, 2015
    Assignee: LYTIX BIOPHARMA AS
    Inventors: Liv Tone Eliassen, Gerd Berge, Baldur Sveinbjørnsson, John Sigurd Svendsen, Øystein Rekdal
  • Patent number: 9096888
    Abstract: A method of detecting sulfotransferase activity including conducting a first reaction comprising, measuring free phosphate produced by the first reaction, and comparing the measured free phosphate to a free phosphate standard curve or equation or to a free phosphate level obtained in a separate reaction. The first reaction includes combining the sulfotransferase, Golgi-resident PAP-phosphatase (gPAPP), a 5?-nucleotidase, a substrate of the sulfotransferase, and 3?-phosphoadenosine-5?-phosphosulfate (PAPS) under conditions to produce 3?-phosphoadenosine-5?-phosphate (PAP).
    Type: Grant
    Filed: July 23, 2014
    Date of Patent: August 4, 2015
    Assignee: Research & Diagnostics Systems, Inc.
    Inventor: Zhengliang L. Wu
  • Publication number: 20150141361
    Abstract: A pharmaceutical composition including at least one epigenome-modifying compound, for a use thereof in the treatment of genetic muscular diseases linked to a conformational disorder of at least one protein, said disorder causing the cellular degradation of the protein.
    Type: Application
    Filed: July 16, 2013
    Publication date: May 21, 2015
    Applicant: GENETHON
    Inventor: Isabelle Richard
  • Publication number: 20150140585
    Abstract: Microbial biosensors that generate gas outputs in hard-to-image materials using exogenous methyl halide transferase (MHT) genes. By varying the promoter that is fused to the MHT gene, biosensors for different triggers can be made.
    Type: Application
    Filed: October 10, 2014
    Publication date: May 21, 2015
    Inventors: Jonathan J. SILBERG, Caroline A. MASIELLO, Hsiao-Ying CHENG
  • Publication number: 20150140010
    Abstract: The present invention relates to methods for diagnosing and treating focal segmental glomerulosclerosis. More particularly, the present invention relates to a method for determining whether a subject is at risk of having or developing a focal segmental glomerulosclerosis (FSGS) comprising the step consisting of determining the level of calcium/calmodulin-dependent serine protein kinase (CASK) in a blood sample obtained from the subject. The present invention also relates to an agent effective to inhibit the binding of CASK to hCD98 present on the surface of podocytes for use in the prevention or treatment of focal segmental glomerulosclerosis (FSGS) in a subject in need thereof.
    Type: Application
    Filed: May 22, 2013
    Publication date: May 21, 2015
    Applicants: INSERM 9INSTITUT NATIONAL DE LA SANTE ET DE LA R- ECHERCHE MEDICALE), UNIVERSITE PARIS-SUD XI, ASSISTANCE PUBLIQUE-HOPITAUX DE PARIS
    Inventors: Antoine Durrbach, Hanz Lorenzo, Severine Beaudreuil