Involving Transferase Patents (Class 435/15)
  • Patent number: 11307204
    Abstract: The present disclosure relates to methods for detecting brain tumors and assessing the recurrence of such tumors by administering a pharmaceutical composition comprising 5-aminolevulinic acid (5-ALA) and detecting the conversion of 5-ALA to protoporphyrin IX (PPIX) associated with brain-derived microparticles.
    Type: Grant
    Filed: June 7, 2019
    Date of Patent: April 19, 2022
    Assignee: PIOMA, INC.
    Inventor: Alan M. Ezrin
  • Patent number: 11273161
    Abstract: Methods of diagnosing and treating autism spectrum disorders are provided.
    Type: Grant
    Filed: June 12, 2019
    Date of Patent: March 15, 2022
    Assignee: The Broad Institute, Inc.
    Inventors: Jon Madison, Jeffrey Cottrell
  • Patent number: 11274325
    Abstract: Provided herein, inter alia, are methods, bacteria, nucleic acids, and polypeptides for producing sialylated oligosaccharides.
    Type: Grant
    Filed: December 14, 2018
    Date of Patent: March 15, 2022
    Assignee: Glycosyn LLC
    Inventors: Srikrishnan Mallipeddi, Matthew Ian Heidtman, Massimo Merighi, John M. McCoy
  • Patent number: 11242550
    Abstract: The present invention discloses a method for preparing glucosyl steviol glycosides through enzymatic catalysis under programming temperatures in high throughput, belonging to the technical field of biosynthesis of sweeteners. By using cyclodextrin glucosyltransferase from Geobacillus sp. as a catalyst, steviol glycosides as the glycosyl receptor and dextrin or oligosaccharide as the glycosyl donor, taking a calcium/barium ion salt bridge as the main stabilizer and combining with glycerol to adjust the conformation and binding domain openness of the enzyme, and utilizing transglucosylation and hydrolytic activities of amylase at variable temperatures in different stages, thereby preparing the glucosyl steviol glycosides through enzymatic catalysis under programming temperatures in high throughput. The technology of the present invention can improve the utilization rate of the enzyme, and obtain glucosyl steviol glycosides with good sweetness and good taste.
    Type: Grant
    Filed: December 5, 2018
    Date of Patent: February 8, 2022
    Assignees: Jiangnan University, DONGTAI HAORUI BIOTECHNOLOGY CO., LTD.
    Inventors: Yongmei Xia, Liping Zhu, Yun Fang, Xiang Liu, Haijun Wang
  • Patent number: 11236061
    Abstract: The present specification provides a compound represented by Chemical Formula 1, a colorant composition, a resin composition, a photosensitive material, a color filter and a display device comprising the same.
    Type: Grant
    Filed: February 26, 2020
    Date of Patent: February 1, 2022
    Assignee: LG CHEM, LTD.
    Inventors: Jongho Park, Dami Lee, Seung Jin Yang, Jihye Jung
  • Patent number: 11220703
    Abstract: Provided herein are analytical methods utilizing a self-limiting catalytic reaction, and compositions and kits useful therefore. In one aspect the method is used to quantify Pd, and in another, the method is a horseradish peroxidase assay.
    Type: Grant
    Filed: November 23, 2016
    Date of Patent: January 11, 2022
    Assignees: University of Pittsburgh—Of the Commonwealth System of Higher Education, Merck Sharp & Dohme Corp.
    Inventors: Xiaodong Bu, Junyong Jo, Kazunori Koide, Matthew Patrick Tracey, Christopher J. Welch
  • Patent number: 11124843
    Abstract: Provided is a method for detecting a bacterium of the genus Nitrobacter, comprising: a first step of amplifying a nucleotide using a test DNA as a template and primers capable of amplifying a nucleotide sequence of consecutive 110 nucleotides or more and 157 nucleotides or less in the nucleotide sequence set forth in SEQ ID NO: 1 to obtain an amplified product; and a second step of detecting the amplified product.
    Type: Grant
    Filed: March 30, 2016
    Date of Patent: September 21, 2021
    Assignee: SUMITOMO CHEMICAL COMPANY, LIMITED
    Inventor: Tomokazu Mitsui
  • Patent number: 11066649
    Abstract: The invention relates to a method for inducing human cholangiocyte differentiation of progenitor cells called hepatoblasts. More specifically, the invention relates to a method for differentiating hepatoblasts to cholangiocytes by culturing said hepatoblasts with a particular medium having interleukin-6 (IL-6) activity. The differentiation method can specifically induce cholangiocyte differentiation from hepatoblasts, and the human cholangiocytes differentiated according to the invention may be useful for drug discovery for treatment of cholangiopathies and bioengineered livers.
    Type: Grant
    Filed: March 19, 2015
    Date of Patent: July 20, 2021
    Assignees: INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM), UNIVERSITÉ PARIS-SACLAY, UNIVERSITE DE RENNES
    Inventors: Anne Dubart Kupperschmitt, Anne Weber Benarous, Anne Corlu, Noushin Dianat
  • Patent number: 11021703
    Abstract: This invention relates to a method of characterizing the modified base status of a transcriptome, which involves contacting a transcriptome comprising one or more modified bases with an antibody specific to the modified bases under conditions effective to bind the antibody to the modified bases; isolating, from the transcriptome, a pool of RNA transcripts to which the antibody binds; and identifying isolated RNA transcripts that are present in a higher abundance in the isolated pool relative to the transcriptome, where each of the isolated RNA transcripts that are present in a higher abundance in the isolated pool together characterize the modified base status of the transcriptome. Also disclosed are a method of diagnosis or prognosis of a disease, a method of determining the effect of a treatment on modified base levels in RNA, and a kit for characterizing the modified base status of a transcriptome.
    Type: Grant
    Filed: February 18, 2013
    Date of Patent: June 1, 2021
    Assignee: CORNELL UNIVERSITY
    Inventors: Samie R. Jaffrey, Kate D. Meyer, Christopher E. Mason
  • Patent number: 10975440
    Abstract: Sets of experimentally validated gene specific primer pairs are provided. Embodiments of the sets include 10 or more gene specific primer pairs of forward and reverse primers. The forward and reverse primers of each primer pair include gene specific primers that are experimentally validated as suitable for use in a multiplex amplification assay. In some instances, each of the forward and reverse primers includes an anchor domain that includes a universal primer binding site. The sets find use in a variety of different applications, including high-throughput sequencing applications.
    Type: Grant
    Filed: April 19, 2016
    Date of Patent: April 13, 2021
    Assignee: Cellecta, Inc.
    Inventors: Alex Chenchik, Costa Frangou, Mikhail Makhanov
  • Patent number: 10883992
    Abstract: The invention provides kinase substrates and methods comprising their use.
    Type: Grant
    Filed: February 22, 2019
    Date of Patent: January 5, 2021
    Assignees: Regents of the University of Minnesota, Yale University
    Inventors: Laurie L. Parker, Wei Cui, Benjamin Turk
  • Patent number: 10870894
    Abstract: Methods for detecting microorganisms, in particular detection of bacteria and methods for measuring enzyme activity, such as Deoxyribonucleic acid (DNA) polymerase activity are disclosed. The aforesaid methods include, but are not limited to such methods performed on microbial crude lysates, useful for determining microbial enzyme activities, which can be linked to amplification signal generators such as real-time Polymerase Chain Reaction (PCR) techniques, thereby enabling determination of microbial pathogens in samples such as unpurified blood and other body fluids. Moreover, the disclosed embodiments also relate to reagents for use in such methods, and to test kits comprising such reagents for carrying out the methods.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: December 22, 2020
    Inventors: Shawn Mark O'Hara, Daniel Zweitzig
  • Patent number: 10865392
    Abstract: The present invention relates to acetyl transferases, nucleic acid sequences coding therefore, expression constructs and vectors comprising these sequences, microorganisms transformed therewith and use thereof.
    Type: Grant
    Filed: October 4, 2019
    Date of Patent: December 15, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Christopher Farrell, Peter Houston, Lisa Laprade, Nathalie Balch, Maria Mayorga
  • Patent number: 10813931
    Abstract: The compositions and methods described herein relate to the treatment of cancer, e.g. by reducing the regression of cancer cells from regressing into cancer stem cell-like phenotypes and/or reducing the development of drug-resistant cancer cells. In some embodiments, the compositions and methods relate to inhibitors of PI3K pathway kinases and Src family kinases.
    Type: Grant
    Filed: February 12, 2014
    Date of Patent: October 27, 2020
    Assignee: THE BRIGHAM AND WOMEN'S HOSPITAL, INC.
    Inventors: Aaron Goldman, Ashish Kulkarni, Shiladitya Sengupta
  • Patent number: 10793894
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Grant
    Filed: February 20, 2019
    Date of Patent: October 6, 2020
    Assignee: BIOMARIN PHARMACEUTICAL INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 10731200
    Abstract: A method of quantifying ammonia, which method includes: performing a first reaction in which a test liquid containing ammonia is reacted with ATP and L-glutamic acid in the presence of glutamine synthetase to produce ADP; performing a second reaction in which the produced ADP is reacted with glucose in the presence of ADP-dependent hexokinase to produce glucose-6-phosphate; performing a third reaction in which the produced glucose-6-phosphate is reacted with an oxidized NAD compound in the presence of glucose-6-phosphate dehydrogenase to produce a reduced NAD compound; and quantifying the reduced NAD compound to quantify ammonia.
    Type: Grant
    Filed: October 19, 2017
    Date of Patent: August 4, 2020
    Assignee: ARKRAY, Inc.
    Inventors: Takehiro Funamoto, Masaki Murakami
  • Patent number: 10724015
    Abstract: The present invention relates to a mutant microorganism in which a gene that encodes phosphofructokinase-2 is disrupted or deleted to reduce glycolytic flux to thereby improve the ability of the microorganism to produce N-acetylglucosamine, and to a method of producing N-acetylglucosamine using the mutant microorganism. The mutant microorganism according to the present invention has advantages in that it has high resistance to various chemical substances, grows rapidly, is easily cultured, and produces N-acetylglucosamine with high efficiency, indicating that it is useful for production of a large amount of N-acetylglucosamine.
    Type: Grant
    Filed: May 17, 2017
    Date of Patent: July 28, 2020
    Assignee: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION
    Inventors: Min-Kyu Oh, Sang-Woo Lee
  • Patent number: 10697870
    Abstract: An object is to provide a blood test kit and a blood analysis method capable of performing a blood test with a small amount of blood at high accuracy by visualizing a volume of blood collection and keeping the volume constant. Provided is a blood test kit for analyzing a concentration of a target component in a blood sample using a normal component homeostatically present in blood, the kit including: a blood collection instrument for collecting the blood sample; a diluent solution for diluting the collected blood sample; and a storing instrument for storing the diluted blood sample, in which the blood collection instrument is a capillary.
    Type: Grant
    Filed: January 3, 2018
    Date of Patent: June 30, 2020
    Assignee: FUJIFILM Corporation
    Inventors: Hikaru Urano, Tatsuya Ishizaka, Haruyasu Nakatsugawa, Susumu Osawa, Shinya Sugimoto
  • Patent number: 10688153
    Abstract: Modified peptides based on C-terminal PDZ binding domains of PTEN and PHLPP, or PDK1 interacting fragment of PKN2 are described as are methods of using the modified peptides for blocking the activity of PTEN, PHLPP and PKN2 and treating sudden cardiac arrest. A method for guiding treatment of cardiac arrest based on sorbitol or taurine levels is also provided.
    Type: Grant
    Filed: November 7, 2016
    Date of Patent: June 23, 2020
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Terry Vanden Hoek, Xiangdong Zhu, Jing Li
  • Patent number: 10677786
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of mucopolysaccharidosis (MPS) I or a disorder associated with MPS I.
    Type: Grant
    Filed: February 8, 2018
    Date of Patent: June 9, 2020
    Assignee: BIOMARIN PHARMACEUTICAL INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 10632150
    Abstract: Disclosed herein is a sterile composition for injection comprised of potassium phosphates having no visible particulate. Also disclosed herein is a manufacturing process for the sterile composition and its use.
    Type: Grant
    Filed: April 19, 2019
    Date of Patent: April 28, 2020
    Assignee: CMP Development LLC
    Inventors: H. Greg Thomas, Richard LeVasseur, Jeffrey S. Kiel, Anthony Reid Pipho
  • Patent number: 10619186
    Abstract: The disclosure provides for methods, compositions, and kits for normalizing nucleic acid libraries, for example sequencing libraries.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: April 14, 2020
    Assignee: Cellular Research, Inc.
    Inventors: Craig Betts, Glenn Fu
  • Patent number: 10596209
    Abstract: The present invention relates to the identification of a group of microorganisms, which are relatively abundant in the microbial communities associated with fruits and vegetables typically consumed raw and therefore transient or permanent members of the human microbiota. The consumption of mixtures of these microbes at relevant doses will produce a beneficial effect in the host by reducing the propensity to diabetes, obesity and metabolic syndrome mediated in part by production of short chain fatty acids to enhance colonic butyrate production. Therapeutic methods of the invention involve the use of live microorganisms or metabolites derived from said microorganisms to establish a microbial composition in the mammalian host that will improve significantly the ability to control weight, reduce the onset of diabetes, obesity and metabolic syndrome, and improve overall health.
    Type: Grant
    Filed: December 28, 2018
    Date of Patent: March 24, 2020
    Assignee: Solarea Bio, Inc.
    Inventors: Gerardo V. Toledo, Tracy Mincer, Jahir Mauricio Gutierrez Bugarin, Jillian DeWalt, Eric Schott, Maria Juliana Soto Giron
  • Patent number: 10590168
    Abstract: The invention provides kinase substrates and methods comprising their use.
    Type: Grant
    Filed: May 23, 2017
    Date of Patent: March 17, 2020
    Assignee: REGENTS OF THE UNIVERSITY OF MINNESOTA
    Inventors: Laurie L. Parker, Minervo Perez
  • Patent number: 10585270
    Abstract: A multi-plane imaging system for imaging multiple reflection planes of a regular or irregular shaped three-dimensional (3D) specimen having top, side and bottom views simultaneously. The system includes an inverted watertight pyramid well having at least four reflective side surfaces for reflecting the specimen side views, wherein each of the at least four reflective sides surfaces define an angle, ?, relative to the base horizontal plane, and wherein each reflective side surface comprises a plurality of reflective zones; A specimen is positioned horizontally equidistant from the at least four reflective side surfaces and positioned vertically from the base horizontal plane a predetermined vertical distance; wherein the predetermined vertical distance, the at least four reflective side surfaces, and the angle ? comprise a reflection image plane for reflecting the specimen bottom views.
    Type: Grant
    Filed: October 25, 2018
    Date of Patent: March 10, 2020
    Assignee: University of Vermont and State Agricultural College
    Inventors: Nathan R Tykocki, Grant Hennig
  • Patent number: 10570176
    Abstract: The present invention relates to the field of polypeptide medicine, and relates to polypeptides against hepatitis B virus X Protein and uses thereof. Specifically, the present invention relates to polypeptides comprising D-amino acids, which have a function of inhibiting hepatitis B virus X protein, and inhibit HBx activities, inhibit replication of hepatitis B virus DNA and expression of related antigens (e.g., HBeAg) at molecular level, cellular level and animal level, and further inhibit hepatitis and hepatic cirrhosis caused by hepatitis B virus infection and liver cancer that occurs on the basis of hepatic cirrhosis. The polypeptides can be widely used in the prevention and treatment of liver diseases after hepatitis B infection, including hepatitis, hepatic cirrhosis, and liver cancer.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: February 25, 2020
    Assignee: Tianjin TOPTECH Bio-Science & Technology Co., Ltd.
    Inventors: Xiaodong Zhang, Lihong Ye
  • Patent number: 10548948
    Abstract: Antifungal pharmaceutical compositions, dosage forms, and methods of use thereof are disclosed.
    Type: Grant
    Filed: February 27, 2018
    Date of Patent: February 4, 2020
    Assignee: The United States of America as represented by the Secretary of the Navy
    Inventors: Nancy Millenbaugh, Jeremy Wesley Gleaton, Dickson Kiprono Kirui
  • Patent number: 10475942
    Abstract: A photodetector cell may include a substrate, and a first contact carried by the substrate and having a first work function value. The photodetector cell may include a second contact carried by the substrate and having a second work function value different from the first work function value, and a semiconductor wire carried by the substrate and having a third work function value between the first and second work function values. The semiconductor wire may be coupled between the first and second contacts and comprising a photodiode junction.
    Type: Grant
    Filed: May 14, 2018
    Date of Patent: November 12, 2019
    Inventors: Manjeri P. Anantram, Md Golam Rabbani, Mahmoud M. Khader, Reza Nekovei, Amit Verma
  • Patent number: 10453554
    Abstract: The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: October 22, 2019
    Assignee: Codexis Mayflower Holdings, Inc.
    Inventor: Richard John Fox
  • Patent number: 10449182
    Abstract: The present invention provides sorafenib analogs for use in a method of treating a disease mediated by p21, said method comprising administering to a subject in need thereof a therapeutically effective amount of a compound of formula I. The present invention also provides methods of inhibiting p21 in a cell comprising contacting the cell with an effective amount of a compound of formula I.
    Type: Grant
    Filed: June 20, 2013
    Date of Patent: October 22, 2019
    Assignee: The Regents of the University of California
    Inventors: Robert H. Weiss, Bruce D. Hammock, Hiromi Inoue Wettersten, Sung Hee Hwang
  • Patent number: 10422006
    Abstract: Phosphatidylinositol 3-kinases (PI3Ks) are known to be important regulators of signaling pathways. To determine whether PI3Ks are genetically altered in cancers, we analyzed the sequences of the PI3K gene family and discovered that one family member, PIK3CA, is frequently mutated in cancers of the colon and other organs. The majority of mutations clustered near two positions within the PI3K helical or kinase domains. PIK3CA represents one of the most highly mutated oncogenes yet identified in human cancers and is useful as a diagnostic and therapeutic target.
    Type: Grant
    Filed: October 31, 2014
    Date of Patent: September 24, 2019
    Assignee: The John Hopkins University
    Inventors: Yardena Samuels, Victor Velculescu, Kenneth W. Kinzler, Bert Vogelstein
  • Patent number: 10407735
    Abstract: The present invention relates to compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present invention relates to ncRNAs as diagnostic markers and clinical targets for prostate, lung, breast and pancreatic cancer.
    Type: Grant
    Filed: May 17, 2016
    Date of Patent: September 10, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Arul Chinnaiyan, John Prensner, Matthew Iyer
  • Patent number: 10405810
    Abstract: A tool for predicting that a person is likely to be abnormally hydrated over a future time interval, and in some cases to alert the person or a medical professional to intervene. From a series of physiological measurements, a tissue electrical impedance spectrum curve, which comprises a phase spectrum curve in an embodiment, is determined and changes in the shape of the curve are ascertained. The measurements may be received using one or more sensors worn by the person. In some embodiments, current and historic spectrum curvature are applied to an evolutionary algorithm, such as particle-swarm optimization (PSO) or differential evolution (DE), to determine an inference regarding the persons future hydration status. In one embodiment, a statistical forecast for the next epoch immediately beyond the present one, is determined.
    Type: Grant
    Filed: May 23, 2016
    Date of Patent: September 10, 2019
    Assignee: Cerner Innovation, Inc.
    Inventor: Douglas S. McNair
  • Patent number: 10364425
    Abstract: The invention is related to a composition of recombinant or transgenic Factor VII, each molecule of Factor VII of the composition exhibiting two N-glycosylation sites, wherein, among all the molecules of FVII of the composition, the rate of Gal?1,3G al glycan moieties is comprised between 0 and 4%. The invention is also related to a process for preparing such a composition of FVII.
    Type: Grant
    Filed: May 2, 2013
    Date of Patent: July 30, 2019
    Assignee: LABORATOIRE FRANCAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES
    Inventors: Abdessatar Sami Chtourou, Emmanuel Nony, Nicolas Bihoreau
  • Patent number: 10316349
    Abstract: The present invention is a method for measuring the amount of at least one molecule in a biological sample, the method comprising a) combining the sample, or a derivative thereof, with one or more aptamers and allowing one or more molecules in the sample to bind to the aptamer(s); b) separating bound from unbound molecules; and c) quantifying the molecule(s) bound to the or each aptamer, wherein quantification of the bound molecule(s) is carried out by sequencing at least part of the or each aptamer. Uses of and products derived from the method are also contemplated.
    Type: Grant
    Filed: August 4, 2017
    Date of Patent: June 11, 2019
    Assignee: Caris Science, Inc.
    Inventors: Clive Gavin Brown, Koen Kas, Sven Agnes Jan Eyckerman
  • Patent number: 10294240
    Abstract: [Problem] To provide a fluorescent probe that detects calpain activity in cells at high sensitivity. [Solution] A compound represented by the following general formula (I) or a salt thereof.
    Type: Grant
    Filed: February 29, 2016
    Date of Patent: May 21, 2019
    Assignees: The University of Tokyo, Tohoku University
    Inventors: Yasuteru Urano, Yuri Nagayo, Mako Kamiya, Toru Nakazawa
  • Patent number: 10287571
    Abstract: The present invention relates to a modified microorganism having, compared to its wildtype, —a reduced activity of an enzyme encoded by the ptsA-gene, —a reduced activity of an enzyme encoded by the ptsH-gene or —a reduced activity of an enzyme encoded by the ptsA-gene and a reduced activity of an enzyme encoded by the ptsH-gene, wherein the wildtype from which the modified microorganism has been derived belongs to the family of Pasteurellaceae. The present invention also relates to a method for producing succinic acid and to the use of modified microorganisms.
    Type: Grant
    Filed: May 7, 2015
    Date of Patent: May 14, 2019
    Assignee: BASF SE
    Inventors: Joanna Martyna Krawczyk, Stefan Haefner, Hartwig Schroeder, Oskar Zelder
  • Patent number: 10287590
    Abstract: The invention provides methods for introducing co-varying paired nucleic acids into a vector such under separate transcriptional control. An oligonucleotide is synthesized including the paired nucleic acids. The oligonucleotide is then assembled with a spacer nucleic acid encoding a promoter. After assembly the spacer nucleic acid is to one side of the oligonucleotide encoding the paired segments. However, on circularization of the assembled nucleic acid and cleavage between the DNA segments the spacer oligonucleotide and its components now occur between the paired segments. The resulting nucleic acid can now be cloned into a vector with a single step, such that each of the paired nucleic acid segments is linked to its own promoter. The present method can readily be extended to library screening without proportionately increasing the effort.
    Type: Grant
    Filed: December 24, 2014
    Date of Patent: May 14, 2019
    Assignee: DNA2.0, INC.
    Inventors: Jeremy Minshull, Sridhar Govindrajan, Kedar Patel
  • Patent number: 10260084
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: April 16, 2019
    Assignee: BIOMARIN PHARMACEUTICAL INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 10233439
    Abstract: The present invention discloses a directionally modified glucosamine synthase mutant and its application. The amino acid sequence of the glucosamine synthase mutant is as shown in sequence list SEQ ID No. 1, and the nucleotide sequence is as shown in sequence list SEQ ID No. 2. The genetic engineering bacteria of glucosamine synthase is successfully constructed. In order to improve the tolerance of recombinant bacteria against glucosamine, the glucosamine synthase is directionally modified. A glucosamine synthase mutant is selected from the mutant library via high-throughput screening method, the amino acid changes in the mutant induces the spatial conformational change in the enzyme, so as enlarged the region where the enzyme and substrate combines, therefor the combination efficiency of the enzyme and the substrate is increased. The glucosamine synthase of the present invention has various advantages, such as rich in raw material of glucose, and a convenient subsequent extraction.
    Type: Grant
    Filed: June 5, 2017
    Date of Patent: March 19, 2019
    Assignees: Yangzhou Rixing Bio-Tech Co., Ltd, Jiangnan University
    Inventors: Jinsong Shi, Zhenghong Xu, Chao Zhang, Jinsong Gong, Heng Li, Zhenzhong Ding, Xiang Fang, Wanhong Zhang
  • Patent number: 10196692
    Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.
    Type: Grant
    Filed: September 18, 2015
    Date of Patent: February 5, 2019
    Assignee: QIAGEN MANCHESTER LIMITED
    Inventors: Ruth Board, Jennifer Haworth, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
  • Patent number: 10190171
    Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.
    Type: Grant
    Filed: September 18, 2015
    Date of Patent: January 29, 2019
    Assignee: QIAGEN MANCHESTER LIMITED
    Inventors: Ruth Board, Jennifer Haworth, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
  • Patent number: 10175226
    Abstract: A method for quantifying acetylamantadine in a urine sample comprises eluting acetylamantadine from the urine sample using solid phase extraction and quantifying the acetylamantadine eluted from the urine sample using Raman spectroscopy.
    Type: Grant
    Filed: July 10, 2017
    Date of Patent: January 8, 2019
    Assignee: BioMark Technologies, Inc.
    Inventors: Reuven Gordon, Brian Cheng, Rashid Bux, Bram Ramjiawan, Aftab Ahmed, Fraser Alan Hof
  • Patent number: 10077244
    Abstract: A method to detect the presence of amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: September 18, 2018
    Assignee: PROMEGA CORPORATION
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • Patent number: 10059955
    Abstract: The invention relates to isolated nucleic acids encoding a feruloyl-CoA:monolignol transferase and feruloyl-CoA:monolignol transferase enzymes. The isolated nucleic acids and/or the enzymes enable incorporation of monolignol ferulates into the lignin of plants, where such monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate. The invention also includes methods and plants that include nucleic acids encoding a feruloyl-CoA:monolignol transferase enzyme and/or feruloyl-CoA:monolignol transferase enzymes.
    Type: Grant
    Filed: October 4, 2016
    Date of Patent: August 28, 2018
    Assignees: Board of Trustees of Michigan State University, The University of British Columbia, Wisconsin Alumni Resarch Foundation
    Inventors: Curtis Wilkerson, John Ralph, Saunia Withers, Shawn D. Mansfield
  • Patent number: 10035974
    Abstract: The objective of the present invention is to provide a cleaning agent composition which can be used in the open air without any problems since the cleaning agent is environmentally friendly and which is effective for removing a low-molecular-weight and poorly-water-soluble cyclic silicone compound that causes a stain of a silicone sealing. The cleaning agent for washing out a silicone stain according to the present invention is characterized in comprising a cyclic lipopeptide biosurfactant, wherein the specific silicone stain compound such as D4 compound is the objective compound to be removed.
    Type: Grant
    Filed: March 25, 2014
    Date of Patent: July 31, 2018
    Assignees: KANEKA CORPORATION, NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Koji Noda, Masashi Izumida, Satohiro Yanagisawa, Tohru Nakashima, Takuto Nagano, Toshiaki Taira, Tomohiro Imura, Dai Kitamoto
  • Patent number: 9997656
    Abstract: A photodetector cell may include a substrate, and a first contact carried by the substrate and having a first work function value. The photodetector cell may include a second contact carried by the substrate and having a second work function value different from the first work function value, and a semiconductor wire carried by the substrate and having a third work function value between the first and second work function values. The semiconductor wire may be coupled between the first and second contacts and comprising a photodiode junction.
    Type: Grant
    Filed: December 17, 2015
    Date of Patent: June 12, 2018
    Inventors: Manjeri P. Anantram, Md Golam Rabbani, Mahmoud M. Khader, Reza Nekovei, Amit Verma
  • Patent number: 9982242
    Abstract: Provided are: a protein complex capable of selectively and asymmetrically oxidizing an enantiomer of a secondary alcohol without adding a coenzyme and having an asymmetric oxidation activity in a water-soluble solvent system in the presence of oxygen; a method for producing the same; and a method for coating the protein complex with a high molecular weight compound. The method for producing the protein complex includes: (1) enclosing a crude water-soluble protein in a gel, air-oxidizing the gel, and eluting the protein complex into an aqueous solution; and (2) applying gravity to concentrate and precipitate the protein complex, redissolving the precipitate in an aqueous glycine sodium hydroxide solution of about 0.5 mM and allowing the same to homogeneously coexist with a high molecular weight compound, and re-precipitating the solution and dehydrating and drying the same to yield a protein complex coated with a high molecular weight compound.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: May 29, 2018
    Assignee: SANYO FOODS CO., LTD.
    Inventor: Hiroyuki Nagaoka
  • Patent number: 9915649
    Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.
    Type: Grant
    Filed: April 12, 2016
    Date of Patent: March 13, 2018
    Assignee: BIOMARIN PHARMACEUTICALS INC.
    Inventors: Brett E. Crawford, Jillian R. Brown, Charles A. Glass, Jim R. Beitel, Robin M. Jackman
  • Patent number: 9896666
    Abstract: The present invention relates to a method for stabilizing an enzyme by storing the enzyme in the presence of a stable coenzyme. The present invention further relates to an enzyme stabilized with a stable coenzyme, and to the use thereof in test elements for detecting analytes.
    Type: Grant
    Filed: August 19, 2010
    Date of Patent: February 20, 2018
    Assignee: ROCHE DIABETES CARE, INC.
    Inventors: Carina Horn, Dieter Heindl, Claudia Gaessler-Dietsche, Joachim Hoenes