Aromatic Patents (Class 435/156)
  • Patent number: 10738296
    Abstract: The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency. (A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) from Pantoea ananatis consisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids. (B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or mere other amino acids.
    Type: Grant
    Filed: January 10, 2019
    Date of Patent: August 11, 2020
    Assignee: GREEN CHEMICALS CO., LTD.
    Inventors: Masayuki Inui, Kazumi Hiraga, Masako Suda, Ryoma Hashimoto
  • Patent number: 10385328
    Abstract: Genetically engineered cells and methods are presented that allow for the production of various value products from CO2. Contemplated cells have a CBB cycle that is genetically modified such that two molecules of CO2 fixed in the CBB cycle can be withdrawn from the modified CBB cycle as a single C2 compound. In contemplated aspects a CBB cycle includes an enzymatic activity that generates the single C2 compound from a compound of the CBB cycle, while further modifications to the CBB cycle will not introduce additional recombinant enzymatic activity/activities outside the already existing catalytic activities in the CBB cycle.
    Type: Grant
    Filed: March 28, 2018
    Date of Patent: August 20, 2019
    Assignee: Easel Biotechnologies, LLC
    Inventors: Yi-Xin Huo, Benjamin Schilling, Shahrooz Rabizadeh
  • Patent number: 10208293
    Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express a mutant AROM polypeptide and/or mutant catechol-O-methyltransferase polypeptide alone or in combination with one or more vanillin biosynthetic enzymes or UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce vanillin or vanillin beta-D-glucoside.
    Type: Grant
    Filed: August 7, 2012
    Date of Patent: February 19, 2019
    Assignees: International Flavors & Fragrances Inc., Evolva SA
    Inventors: Joergen Hansen, Esben Halkjaer Hansen, Honey Polur, Joseph M. Sheridan, Jonathan R. Heal, William D. O. Hamilton
  • Patent number: 10184138
    Abstract: The present disclosure provides recombinant bacteria with elevated production of ethanol and/or n-butanol from ethylene. Methods for the production of the recombinant bacteria, as well as for use thereof for production of ethanol and/or n-butanol are also provided.
    Type: Grant
    Filed: June 9, 2015
    Date of Patent: January 22, 2019
    Assignee: The Regents of the University of California
    Inventors: Shota Atsumi, Michael D. Toney, Gabriel M. Rodriguez, Yohei Tashiro, Justin B. Siegel, D. Alexander Carlin, Irina Koryakina, Shuchi H. Desai
  • Patent number: 10066252
    Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductase thereby increasing the production of vanillin or vanillin beta-D-glucoside.
    Type: Grant
    Filed: May 15, 2018
    Date of Patent: September 4, 2018
    Assignee: INTERNATIONAL FLAVORS & FRAGRANCES INC.
    Inventors: Esben Halkjaer Hansen, Swee Chuang Lim Hallwyl, Klavs Riishede Hansen
  • Patent number: 10000782
    Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductase thereby increasing the production of vanillin or vanillin beta-D-glucoside.
    Type: Grant
    Filed: July 11, 2014
    Date of Patent: June 19, 2018
    Assignee: INTERNATIONAL FLAVORS & FRAGRANCES INC.
    Inventors: Esben Halkjaer Hansen, Swee Chuang Lim Hallwyl, Klavs Riishede Hansen
  • Patent number: 9844521
    Abstract: The present invention provides a pharmaceutical composition for preventing or treating learning disorders, memory disorders, Parkinson's disease, or ischemic cerebrovascular disease, which comprises a ginger extract or shogaol; and a pharmaceutically acceptable carrier. And also, the present invention provides a food composition for improving learning disorders or memory disorders or alleviating the symptoms of learning disorders or memory disorders which comprises a ginger extract or shogaol as an active ingredient, or a food composition for improving learning or memory which comprises a ginger extract or shogaol as an active ingredient.
    Type: Grant
    Filed: November 13, 2009
    Date of Patent: December 19, 2017
    Assignee: UNIVERSITY-INDUSTRY COOPERATION GROUP OF KYUNG HEE UNIVERSITY
    Inventors: Sun-Yeou Kim, Sang-Keun Ha, Jong-Hoon Ryu, Myung-Sook Oh, Seo-Young Jeong
  • Patent number: 9834793
    Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.
    Type: Grant
    Filed: April 20, 2015
    Date of Patent: December 5, 2017
    Assignee: EVOLVA SA
    Inventors: Michael Katz, Hans Peter Smits, Jochen Forster, Jens Bredal Nielsen
  • Patent number: 9765324
    Abstract: A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin, remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol.
    Type: Grant
    Filed: October 4, 2013
    Date of Patent: September 19, 2017
    Assignee: CORNELL UNIVERSITY
    Inventors: Stephane C. Corgie, Xiaonan Duan, Emmanuel Giannelis, Daniel Aneshansley, Larry P. Walker
  • Patent number: 9719072
    Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Thielavia hyrcaniae.
    Type: Grant
    Filed: October 8, 2013
    Date of Patent: August 1, 2017
    Assignee: Novozymes A/S
    Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
  • Patent number: 9637763
    Abstract: The invention relates to the production of aromatic molecules in prokaryotic and eukaryotic hosts such as E. coli, yeasts, filamentous fungi, algae, microalgae, other plant cells.
    Type: Grant
    Filed: June 22, 2012
    Date of Patent: May 2, 2017
    Assignee: RHO Renewables, Inc.
    Inventor: Philip J. Barr
  • Patent number: 9481898
    Abstract: The disclosure provides a process that converts CO2 to higher alcohols (e.g. isobutanol) using electricity as the energy source. This process stores electricity (e.g. from solar energy, nuclear energy, and the like) in liquid fuels that can be used as high octane number gasoline substitutes. Instead of deriving reducing power from photosynthesis, this process derives reducing power from electrically generated mediators, either H2 or formate. H2 can be derived from electrolysis of water. Formate can be generated by electrochemical reduction of CO2. After delivering the reducing power in the cell, formate becomes CO2 and recycles back. Therefore, the biological CO2 fixation process can occur in the dark.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: November 1, 2016
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: James C. Liao, Kwang Myung Cho
  • Patent number: 9267155
    Abstract: The present invention provides a method of producing sclareol, the method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, the method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing the nucleic acid, as well as a non-human organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: February 23, 2016
    Assignee: FIRMENICH SA
    Inventor: Michel Schalk
  • Patent number: 9260404
    Abstract: The present invention discloses eight new diterpenoids, i.e. Dysongensins A to H, extracted from the leaves and twigs of Dysoxylurn hongkongense, wherein AMBROX® which is applicable in the perfume industry is prepared from Dysongensin A via a series of chemical reactions, and the cytotoxicity of Dysongensins A to H against human cancer cell lines and their antiviral and anti-inflammatory activities are determined. Therefore, in the present invention, AMBROX® prepared from Dysongensin A is a new idea for application as an odorous compound in the perfume industry, and the novel diterpenoids can be prepared as a pharmaceutical compositions and/or a drug having antiviral, anti-inflammatory and/or anti-cancer activities.
    Type: Grant
    Filed: November 20, 2014
    Date of Patent: February 16, 2016
    Assignee: NATIONAL TAIWAN UNIVERSITY
    Inventor: Ya-Ching Shen
  • Patent number: 9150889
    Abstract: The disclosure provides a process that converts CO2 to higher alcohols (e.g. isobutanol) using electricity as the energy source. This process stores electricity (e.g. from solar energy, nuclear energy, and the like) in liquid fuels that can be used as high octane number gasoline substitutes. Instead of deriving reducing power from photosynthesis, this process derives reducing power from electrically generated mediators, either H2 or formate. H2 can be derived from electrolysis of water. Formate can be generated by electrochemical reduction of CO2. After delivering the reducing power in the cell, formate becomes CO2 and recycles back. Therefore, the biological CO2 fixation process can occur in the dark.
    Type: Grant
    Filed: January 15, 2011
    Date of Patent: October 6, 2015
    Assignee: The Regents of the University of California
    Inventors: James C. Liao, Kwang Myung Cho
  • Patent number: 9040269
    Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.
    Type: Grant
    Filed: April 17, 2008
    Date of Patent: May 26, 2015
    Assignee: Evola SA
    Inventors: Michael Katz, Hans Peter Smits, Jochen Förster, Jens Bredal Nielsen
  • Publication number: 20150128301
    Abstract: Nucleic acid molecules from cannabis has been isolated and characterized and encode polypeptides having aromatic prenyltransferase activity. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.
    Type: Application
    Filed: October 9, 2014
    Publication date: May 7, 2015
    Inventors: Jonathan E. Page, Zakia Boubakir
  • Patent number: 8936924
    Abstract: A method for using a thermostable phytase for eliminating or reducing phytic acid or salts of phytic acid in an alcohol production process is disclosed. The phytase can be added anywhere in the alcohol production process including a feedstock, a hammer mill, a slurry tank, a jet cooker, a liquefaction, a mash cooker, a fermentation, a beer, a distillation system, a whole stillage, a centrifuge, a thin stillage, an evaporator, a condensate, a syrup, a wet grain, a drum dryer, a distillers dried grain, distillers solubles, distillers wet grain, condensed distillers solubles distillers dried grains with solubles a molecular sieves, or any combination thereof. The alcohol production process can be in an ethanol production plant; a spirit or a drinkable alcohol production plant; or a fuel ethanol plant.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: January 20, 2015
    Assignee: Verenium Corporation
    Inventors: Arne Solbak, Brian Steer, Mark Dycaico, Katie A. Kline, Axel Trefzer, Thomas Todaro, Fatima El-Farrah, Alberto Alvarado, Gerhard Frey
  • Publication number: 20150017695
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
    Type: Application
    Filed: September 30, 2014
    Publication date: January 15, 2015
    Inventors: Jack Liang, Stephane J. Jenne, Emily Mundorff, Charlene Ching, John M. Gruber, Anke Krebber, Gjalt W. Huisman
  • Publication number: 20140363858
    Abstract: Provided herein are methods for producing an ortho-hydroxylated phenylpropanoid. In one embodiment the method includes culturing a microbe that includes HpaBC activity in the presence of a phenylpropanoid substrate. Also provided are genetically engineered microbes engineered to have greater levels of HpaB and/or HpaC than a control microbe.
    Type: Application
    Filed: June 10, 2014
    Publication date: December 11, 2014
    Inventors: YAJUN YAN, YUHENG LIN
  • Patent number: 8895287
    Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.
    Type: Grant
    Filed: February 21, 2006
    Date of Patent: November 25, 2014
    Assignee: Evolva SA
    Inventors: Michael Katz, Hans Peter Smits, Jochen Förster, Jens Bredal Nielsen
  • Publication number: 20140315269
    Abstract: The present invention relates to a method for producing polyphenol compounds, i.e. phloroglucinol or one of its derivatives, with a polyketide synthase of type III (PKSIII) from a brown marine alga. The invention also relates to recombinant nucleic acids coding for a polyketide synthase of type III (PKSIII) from the brown alga Ectocarpus siliculosus (E. siliculosus), to recombinant vectors comprising these nucleic acids, as well as to host cells comprising these vectors. Finally, the invention relates to a method for preparing of various compounds by means of polyphenol compounds produced according to the aforementioned method.
    Type: Application
    Filed: September 26, 2012
    Publication date: October 23, 2014
    Applicants: UNIVERSITE DE BRETAGNE OCCIDENTALE, UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6)
    Inventors: Ludovic Delage, Laurence Meslet-Cladiere, Philippe Potin, Sophie Goulitquer
  • Publication number: 20140308731
    Abstract: Two novel cytochrome P450 genes are isolated from sorghum, each gene encoding a protein having pentadecatrienyl resorcinol hydroxylase activity. Expression vectors containing these sequences are made and used to elevate levels of pentadecatrienyl resorcinol hydroxylase in transgenic cells and organisms.
    Type: Application
    Filed: April 15, 2013
    Publication date: October 16, 2014
    Inventor: The United States of America, as Represented by the Secretary of Agriculture
  • Publication number: 20140234918
    Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: August 10, 2012
    Publication date: August 21, 2014
    Applicant: Novozymes A/S
    Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
  • Publication number: 20140220648
    Abstract: The invention relates to the production of aromatic molecules in prokaryotic and eukaryotic hosts such as E. coli, yeasts, filamentous fungi, algae, microalgae, other plant cells.
    Type: Application
    Filed: June 22, 2012
    Publication date: August 7, 2014
    Applicant: RHO Renewables
    Inventor: Philip J. Barr
  • Patent number: 8796002
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ? chloroalcohols from ? chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.
    Type: Grant
    Filed: June 22, 2010
    Date of Patent: August 5, 2014
    Assignee: Codexis, Inc.
    Inventors: Yong Koy Bong, Michael Vogel, Steven J. Collier, Vesna Mitchell, Jagadeesh Mavinahalli
  • Publication number: 20140206051
    Abstract: A method for the production of a stilbenoid, such as resveratrol or pinosylvin, by fermenting plant material such a grape must using a yeast having a metabolic pathway producing said stilbenoid, separating a solids waste material from said fermentation and extracting said stilbenoid.
    Type: Application
    Filed: October 15, 2013
    Publication date: July 24, 2014
    Applicant: Evolva SA
    Inventors: Bo STENHUUS, Hans Peter SMITS, Thomas DURHUUS, Michael KATZ
  • Publication number: 20140199741
    Abstract: A process for manufacturing tetrahydrofuran, tetrahydropyran and, more generally, cycloether derivatives through the cyclodehydration of 1,4- or 1,5-diols. More specifically, the process of the invention involves (i) the stereoselective cyclodehydration in water of 1,4- or 1,5-diols including at least one chiral tertiary alcohol functional group with retention of the initial chirality, and/or (ii) the cyclodehydration in water of 1,4- or 1,5-diols, said diols being non-miscible with and/or non-soluble in water, into corresponding cycloether derivatives, by bringing the reaction mixture to high temperature water (HTW) conditions and/or by mixing the aqueous reaction mixture with a solid catalyst, such as for example a smectite clay. Also, the use of the process for manufacturing ambrafuran, especially (?)-ambrafuran and other cycloether derivatives.
    Type: Application
    Filed: July 13, 2012
    Publication date: July 17, 2014
    Applicant: KOSTE BIOCHEMICALS
    Inventor: Charles Carey
  • Publication number: 20140170720
    Abstract: The present invention relates to a method for producing retinoid from a microorganism, and more specifically, to a method for effectively obtaining retinoid, which lacks stability, from a microorganism by cultivating the microorganism capable of producing retinoid in a medium containing a lipophilic substance, and separating retinoid from the lipophilic substance.
    Type: Application
    Filed: July 30, 2012
    Publication date: June 19, 2014
    Applicant: INDUSTRY-ACADEMIC COOPERATION FOUNDATION GYEONGSANG NATIONAL UNIVERSITY
    Inventors: Seon Won Kim, Hui Jeong Jang, Sang Hwal Yoon, Bo Kyung Ha, Hee Kyung Ryu
  • Publication number: 20140163142
    Abstract: Disclosed herein are method to modify the lignin with particular fungal species, and procedure to synthesize phenolic adhesives with the modified lignin as raw materials, and the adhesives compositions and methods for making adhesive compositions, and methods for making lingo-cellulosic composites from renewable materials. Four fungi in examples are Lenzites elegans (Spreng.) Pat. (FTK 329A), Phanerochaete cremea (Bres.) Parmasto (FTK 332A), Pycnoporellus alboluteus (Ellis & Everh.) Kotl. & Pouz. (FTK 76A) and Meruliopsis taxicola (Pers.) Bondartsev (FTK 122B). Lignin used in examples are organosolv lignin, Kraft lignin, and ammonium lignosulfonate. The present invention includes methods to (1) modify of lignin with fungi; (2) in-situ polymerize modified lignin-phenol-formaldehyde to generate bio-modified lignin-phenol-formaldehyde adhesive in liquid form, and (3) manufacture composite panels with bio-modified lignin-phenol-formaldehyde resins.
    Type: Application
    Filed: February 17, 2012
    Publication date: June 12, 2014
    Applicant: FPINNOVATIONS
    Inventors: Yaolin Zhang, Dian-Qing Wang, Xiang-Ming Wang, Martin Feng, Gilles Brunette
  • Publication number: 20140147886
    Abstract: A method to increase the production of products of interest in plant material including plant cultures, such as, for example, cell suspension cultures, root cultures, and hairy root cultures is provided. In one embodiment, the method is to contacting the plant material with a precursor or xenobiotic when producing a product of interest from a plant. In another embodiment the plant material is also contacted with a trapping agent. The process may also provide for contacting an elicitor of the product of interest with the plant material. An embodiment provides for contacting an elicitor, precursor and trapping agent with the plant material. The ability to produce novel compounds such as glucosides and glucuronides is provided.
    Type: Application
    Filed: March 5, 2013
    Publication date: May 29, 2014
    Applicant: ARKANSAS STATE UNIVERSITY
    Inventors: Luis Fabricio Medina-Bolivar, Tianhong Yang
  • Patent number: 8735137
    Abstract: Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.
    Type: Grant
    Filed: November 15, 2011
    Date of Patent: May 27, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventors: Seung-Pyo Hong, John E. Seip, Quinn Qun Zhu
  • Publication number: 20140141476
    Abstract: Polypeptides having alkanoyl-CoA activity have been identified and characterized, as have nucleic acids encoding these polypeptides. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds in organisms. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.
    Type: Application
    Filed: July 13, 2012
    Publication date: May 22, 2014
    Applicants: UNIVERSITY OF SASKATCHEWAN, NATIONAL RESEARCH COUNCIL OF CANADA
    Inventors: Jonathan E. Page, Jason M. Stout
  • Publication number: 20140134689
    Abstract: The present invention provides for a method of producing an oxidation product of an aromatic amino acid in a genetically modified host cell. The method comprises culturing the genetically modified host cell under a suitable condition such that the culturing results in the genetically modified host cell producing oxidation product of an aromatic amino acid. The host cell comprises an enzyme capable of catalyzing the oxidation of aromatic amino acid. In some embodiments, the host cell is capable of biosynthesizing BH4 or MH4 from GTP.
    Type: Application
    Filed: September 30, 2013
    Publication date: May 15, 2014
    Applicant: The Regents of the University of California
    Inventors: Taek Soon Lee, Yasuharu Satoh, Jay D. Keasling
  • Patent number: 8709751
    Abstract: The present invention relates to the preparation of isomerically pure substituted cyclohexanols starting from a mixture of cis/trans substituted cyclohexanols which comprises reacting the cis/trans mixture of a substituted cyclohexanol with a dicarboxylic acid anhydride in the presence of a lipase, to give the trans semi-ester which is separated off from the unreacted substituted cyclohexanol cis isomer.
    Type: Grant
    Filed: November 23, 2011
    Date of Patent: April 29, 2014
    Assignee: BASF SE
    Inventors: Melanie Bonnekessel, Klaus Ditrich, Jürgen Däuwel, Achim Sorg, Wolfgang Ladner, Bryan Cooper, Rene Backes
  • Publication number: 20140106423
    Abstract: Provided is a method of preparing piceatannol, and more particularly, to a method of preparing piceatannol from resveratrol using bacterial cytochrome P450 BM3 (CYP102A1) or mutants thereof, and a composition and a kit therefor.
    Type: Application
    Filed: September 30, 2013
    Publication date: April 17, 2014
    Applicant: GLO BIOTECH
    Inventors: Chul Ho YUN, Dong Hyun KIM
  • Publication number: 20140087428
    Abstract: The present invention is concerned with methods for producing vanillin, feroyl-CoA, ferulic acid, coniferyl aldehyde and/or coniferyl alcohol. Also, the invention relates to microorganisms useful in such production method, and to the construction of such microorganisms.
    Type: Application
    Filed: June 18, 2012
    Publication date: March 27, 2014
    Inventors: Stefan Lambrecht, Jens-Michael Hilmer, Manuel Pesaro, Jens Kroll, Gunda Hansen, Alexander Steinbüchel, Christian Fleige
  • Publication number: 20140086879
    Abstract: Disclosed herein is an isolated Muscodor albus strain producing volatile organic compounds such as aristolene, 3-octanone and/or acetic acid ester, as well as cultures of said strain and compositions, metabolites and volatiles derived from said strain or culture as well as methods of obtaining said compositions, metabolites and volatiles and their methods of use for controlling pests. Also disclosed are artificial compositions having the same components and uses as the volatiles derived from the strain. A method for capturing and sampling the volatiles is also disclosed.
    Type: Application
    Filed: September 23, 2013
    Publication date: March 27, 2014
    Applicant: Marrone Bio Innovations, Inc.
    Inventors: Gary Strobel, Vu Phong Bui, Hai Su, Phyllis Himmel, Pamela Marrone, Lijuan Xing, Sarah Lewis
  • Publication number: 20140024862
    Abstract: A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.
    Type: Application
    Filed: January 2, 2013
    Publication date: January 23, 2014
    Inventors: Michael Katz, Jochen Forster, Helga David, Hans Peter Schmidt, Malin Sendelius, Sara Peterson Bjorn, Thomas Thomasen Durhuus
  • Publication number: 20140011244
    Abstract: A method for producing naturally derived beneficial compounds including dispersing a microbiological culture media including at least one live probiotic organism, and at least one nutraceutical and/or at least one nutritive agent in distilled water to form a broth, incubating the broth at a predetermined temperature for a select period of time to induce probiotic activity; halting the probiotic activity, and separating the desired compound from the broth.
    Type: Application
    Filed: September 9, 2013
    Publication date: January 9, 2014
    Applicant: Premier Research Labs, LP
    Inventor: Robert J. Marshall
  • Publication number: 20140004583
    Abstract: A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.
    Type: Application
    Filed: March 9, 2012
    Publication date: January 2, 2014
    Applicant: CORNELL UNIVERSITY
    Inventors: Stephane C. Corgie, Patarawan Kahawong, Emmanuel P. Giannelis, Larry P. Walker
  • Patent number: 8617860
    Abstract: The present invention provides a method of producing sclareol, the method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, the method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing the nucleic acid, as well as a non-human organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.
    Type: Grant
    Filed: January 26, 2009
    Date of Patent: December 31, 2013
    Assignee: Firmenich SA
    Inventor: Michel Schalk
  • Patent number: 8617854
    Abstract: The present invention relates to a multi-stage process for producing substituted, optically active alcohols, comprising an enzyme-catalyzed synthesis step, in particular a synthesis step which is catalyzed by an alcohol dehydrogenase. The inventive method is particularly suitable for producing phenylephrine, i.e. 3-[(1R)-1-hydroxy-2-methylamino-ethyl]-phenol.
    Type: Grant
    Filed: September 15, 2009
    Date of Patent: December 31, 2013
    Assignee: BASF SE
    Inventors: Michael Breuer, Andreas Pletsch, Bernhard Hauer, Wolfgang Siegel
  • Publication number: 20130344552
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
    Type: Application
    Filed: August 19, 2013
    Publication date: December 26, 2013
    Applicant: Codexis, Inc.
    Inventors: Jack Liang, Stephane J. Jenne, Emily Mundorff, Charlene Ching, John M. Gruber, Anke Krebber, Gjalt W. Huisman
  • Patent number: 8580555
    Abstract: The present disclosure provides engineered halohydrin dehalogenase (HHDH) polypeptides having improved enzyme properties as compared to the wild-type HHDH enzyme HheC and other reference engineered HHDH polypeptides. Also provided are polynucleotides encoding the engineered HHDH enzymes, host cells capable of expressing the engineered HHDH enzymes, and methods of using the engineered HHDH enzymes to synthesize a variety of chiral compounds including chiral epoxides and chiral alcohols.
    Type: Grant
    Filed: April 20, 2012
    Date of Patent: November 12, 2013
    Assignee: Codexis, Inc.
    Inventors: Erik De Vries, Louis Clark, Scott McVicar, Erika Segraves, Shiwei Song, Kheng Lin Tan
  • Patent number: 8569024
    Abstract: A method for the production of a stilbenoid, such as resveratrol or pinosylvin, by fermenting plant material such a grape must using a yeast having a metabolic pathway producing said stilbenoid, separating a solids waste material from said fermentation and extracting said stilbenoid.
    Type: Grant
    Filed: April 2, 2009
    Date of Patent: October 29, 2013
    Assignee: Evolva SA
    Inventors: Bo Stenhuus, Hans Peter Smits, Thomas Durhuus, Michael Katz
  • Patent number: 8563292
    Abstract: Crude solid vanillin-containing material is precipitated from a solution obtained by biotransformation, and purified by a process comprising contacting it with a purification fluid selected from (a) a liquefied gas whose pressure exceeds its critical pressure and whose temperature is below its critical temperature; (b) a supercritical fluid; (c) a gas. The fluid is preferably liquid carbon dioxide. The temperature is maintained below 25° . The product may be further purified by treatment with CO2 in a fluid bed drier. The crude material is preferably one precipitated from a solution resulting from biotransformation of ferulic acid. A new strain of Amycolatopsis capable of generating high concentrations of vanillin with minimal odoriferous by-products (e.g. guaiacol) is also disclosed.
    Type: Grant
    Filed: July 14, 2011
    Date of Patent: October 22, 2013
    Assignee: SAF-ISIS
    Inventors: Steve Heald, Steve Myers, Tim Walford, Keith Robbins, Colin Hill
  • Publication number: 20130273624
    Abstract: Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having chorismate-pyruvate lyase activity and a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing a saccharide under reducing conditions, and a step of collecting phenol from the reaction mixture.
    Type: Application
    Filed: November 9, 2011
    Publication date: October 17, 2013
    Applicant: Green Phenol Technology Research Association
    Inventors: Hideaki Yukawa, Masayuki Inui
  • Publication number: 20130266999
    Abstract: Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into Corynebacterium glutamicum as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing 4-hydroxybenzoate or a salt thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.
    Type: Application
    Filed: November 9, 2011
    Publication date: October 10, 2013
    Inventors: Hideaki Yukawa, Masayuki Inui
  • Publication number: 20130269061
    Abstract: Genetically engineered plants having altered levels of one or more starch regulation enzymes and a polysaccharide degrading enzyme are provided. Methods of genetically engineering plants to express products altering expression of one or more starch regulation enzymes and polysaccharide degrading enzymes, and genetic constructs are provided. Methods of agricultural processing and animal feed using the genetically engineered plants are described.
    Type: Application
    Filed: March 11, 2013
    Publication date: October 10, 2013
    Inventors: Philip A. Lessard, Michael Lanahan, Vladimir Samoylov, Oleg Bougri, Jonas Emery, R. Michael Raab, Dongcheng Zhang