Containing Hydroxy Group Patents (Class 435/155)
  • Patent number: 10940510
    Abstract: An additively manufactured component with an internal passage; and a multiple of ultrasonic horns additively manufactured within the internal passage.
    Type: Grant
    Filed: February 1, 2016
    Date of Patent: March 9, 2021
    Assignee: Raytheon Technologies Corporation
    Inventors: Caitlin Oswald, Jesse R Boyer, John P Rizzo, Jr.
  • Patent number: 10894951
    Abstract: Methods and compositions for the oxidation of short alkanes by engineered microorganisms expressing recombinant enzymes is described, along with methods of use.
    Type: Grant
    Filed: November 17, 2016
    Date of Patent: January 19, 2021
    Assignee: Industrial Microbes, Inc.
    Inventors: Elizabeth Jane Clarke, Baolong Zhu, Derek Lorin Greenfield, Stephanie Rhianon Jones, Noah Charles Helman
  • Patent number: 10738328
    Abstract: A method is provided for producing modified mutant yeast and the resulting yeast that can be used as a platform for terpene production. The method includes chemical mutagenesis to effect ergosterol dependent growth in yeast. Subsequently, these yeast are subjected to an erg9 knockout mutation to thereby produce ergosterol dependent growth/erg9 knockout mutation yeast cell lines. The resulting yeast are well suited for use in the production of terpenes.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: August 11, 2020
    Assignee: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION
    Inventors: Joe Chappell, Xun Zhuang, Wu Shuiqin
  • Patent number: 10724059
    Abstract: This invention relates generally to method for producing products, particularly alcohols, by microbial fermentation. In particular, the invention relates to methods for increasing the efficiency of the fermentation, by providing a method for treating the used fermentation broth to produce a treated permeate which is then passed back to the bioreactor. The invention provides a method whereby at least one treatment step used to treat the permeated, produces a gaseous product which is then used in one or more stages of the fermentation process.
    Type: Grant
    Filed: December 5, 2012
    Date of Patent: July 28, 2020
    Assignee: LANZATECH NEW ZEALAND LIMITED
    Inventors: Frank Benkwitz, Christophe Daniel Mihalcea, Alice Marie Havill
  • Patent number: 10696992
    Abstract: The invention provides a genetically modified bacterium for production of thiamine; where the bacterium is characterized by a transgene encoding a thiamine monophosphate phosphatase (TMP phosphatase having EC 3.1.3.-) as well as transgenes encoding polypeptides that catalyze steps in the thiamine pathway. The genetically modified bacterium is characterized by enhanced synthesis and release of thiamine into the extracellular environment. The invention further provides a method for producing thiamine using the genetically modified bacterium of the invention; as well as the use of the genetically modified bacterium for extracellular thiamine production.
    Type: Grant
    Filed: December 16, 2016
    Date of Patent: June 30, 2020
    Assignee: BIOSYNTHIA APS
    Inventors: Luisa Gronenberg, Bo Salomonsen, Matteo Ferla, Hans Jasper Genee
  • Patent number: 10689674
    Abstract: Provided herein are non-naturally occurring microbial organisms comprising a methane-oxidizing metabolic pathway. The invention additionally comprises non-naturally occurring microbial organisms comprising pathways for the production of chemicals. The invention additionally provides methods for using said organisms for the production of chemicals.
    Type: Grant
    Filed: April 14, 2015
    Date of Patent: June 23, 2020
    Assignee: INDUSTRIAL MICROBES, INC.
    Inventors: Noah Helman, Elizabeth Clarke, Derek Greenfield
  • Patent number: 10519471
    Abstract: The present invention provides a biosafety-guarded photobiological butanol production technology based on designer transgenic plants, designer algae, designer blue-green algae (cyanobacteria and oxychlorobacteria), or designer plant cells. The designer photosynthetic organisms are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of butanol (CH3CH2CH2CH2OH) directly from carbon dioxide (CO2) and water (H2O). The butanol production methods of the present invention completely eliminate the problem of recalcitrant lignocellulosics by bypassing the bottleneck problem of the biomass technology.
    Type: Grant
    Filed: June 3, 2017
    Date of Patent: December 31, 2019
    Inventor: James Weifu Lee
  • Patent number: 10472655
    Abstract: There is provided SHC/HAC derivatives, amino acid sequences comprising the SHC/HAC derivatives, nucleotide sequences encoding the SHC/HAC derivatives, vectors comprising nucleotide sequences encoding the SHC/HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC/HAC derivatives and applications of the recombinant host cells comprising either SHC/HAC derivatives or WT SHC/HAC enzymes in methods to prepare (?)-Ambrox and SHC/HAC enzymes in methods to prepare (?)-Ambrox.
    Type: Grant
    Filed: April 22, 2016
    Date of Patent: November 12, 2019
    Assignee: GIVAUDAN S.A.
    Inventors: Eric Eichhorn, Boris Schilling, Denis Wahler, Laurent Fourage, Esther Locher
  • Patent number: 10233471
    Abstract: The invention relates to methods of preconditioning pretreated cellulosic material in the presence of a combination of phenol oxidizing enzyme and glucoamylase. The invention also relates to processes of producing sugars and fermentation products including a preconditioning step. Finally the invention relates to a composition suitable for preconditioning.
    Type: Grant
    Filed: May 15, 2014
    Date of Patent: March 19, 2019
    Assignee: Novozyme A/S
    Inventors: Xin Li, Brandon Emme, Lorraine Putnam, Mads Torry Smith
  • Patent number: 10221204
    Abstract: A composition which has high 4?-GL purity and can be used as a reference standard for various analyses can be obtained by a more convenient method than one conventionally used. A method for preparing a high-purity 4?-GL composition includes the steps of: (A) subjecting a 4?-GL-containing galacto-oligosaccharide to activated carbon column chromatography, and performing stepwise elution with plural organic solvent aqueous solutions, wherein the organic solvent aqueous solutions are used such that the concentration of the organic solvent in the organic solvent aqueous solution is higher than the concentration of the organic solvent in the immediately preceding organic solvent aqueous solution with respect to a series of elutions; and (B) adding an organic solvent to the final fraction eluted in step (A), and crystallizing the 4?-GL.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: March 5, 2019
    Assignee: Kabushiki Kaisha Yakult Honsha
    Inventors: Tetsuya Yamada, Hiroshi Hatano, Kazumasa Kimura, Hidetsugu Sotoya, Yoko Mori
  • Patent number: 10072150
    Abstract: This document describes biochemical pathways for producing 4-hydroxybutyrate, 4-aminobutyrate, putrescine or 1,4-butanediol by forming one or two terminal functional groups, comprised of amine or hydroxyl group, in a C5 backbone substrate such as 2-oxoglutarate or L-glutamate.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: September 11, 2018
    Assignee: INVISTA North America S.à r.l.
    Inventors: Alex Van Eck Conradie, Adriana Leonora Botes
  • Patent number: 9695448
    Abstract: The present invention provides a biosafety-guarded photobiological butanol production technology based on designer transgenic plants, designer algae, designer blue-green algae (cyanobacteria and oxychlorobacteria), or designer plant cells. The designer photosynthetic organisms are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of butanol (CH3CH2CH2CH2OH) directly from carbon dioxide (CO2) and water (H2O). The butanol production methods of the present invention completely eliminate the problem of recalcitrant lignocellulosics by bypassing the bottleneck problem of the biomass technology.
    Type: Grant
    Filed: April 4, 2014
    Date of Patent: July 4, 2017
    Inventor: James Weifu Lee
  • Patent number: 9677092
    Abstract: Integrated processes are disclosed for the anaerobic bioconversion of syngas to alcohol.
    Type: Grant
    Filed: February 7, 2014
    Date of Patent: June 13, 2017
    Assignee: Synata Bio, Inc.
    Inventors: Rathin Datta, Steven G. Calderone, Jianxin Du, Robert Hickey
  • Patent number: 9505795
    Abstract: The disclosure provides a method of producing a scyllo-inositol or a new scyllo-inositol derivative in a one-step process, from ubiquitous and inexpensive raw materials. Also provided is a scyllo-inositol derivative bonded to saccharides such as glucose and similar.
    Type: Grant
    Filed: January 22, 2013
    Date of Patent: November 29, 2016
    Assignee: Asahi Kasei Chemicals Corporation
    Inventors: Kazunobu Konishi, Shinichi Imazu
  • Patent number: 9422583
    Abstract: Stereospecific carbonyl reductases SCR1, SCR2, and SCR3 are described herein as are nucleotide sequences that encode these reductases. These stereospecific carbonyl reductases have anti-Prelog selectivity and have specificities that are useful for fine biochemical synthesis.
    Type: Grant
    Filed: March 2, 2015
    Date of Patent: August 23, 2016
    Assignees: Rutgers, The State University of New Jersey, Jiangnan University
    Inventors: Gaetano T. Montelione, Rong Xiao, Yao Nie, Yan Xu
  • Patent number: 9371547
    Abstract: In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant.
    Type: Grant
    Filed: September 10, 2014
    Date of Patent: June 21, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Keith H. Burlew, Robert Dicosimo, Michael Charles Grady
  • Patent number: 9206448
    Abstract: In an alcohol fermentation process, oil derived from biomass is chemically converted into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be chemically converted into a reaction product, such as fatty acids, fatty alcohols, fatty amides, fatty acid methyl esters, fatty acid glycol esters, and hydroxylated triglycerides, and mixtures thereof, which forms a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be chemically converting into the fermentation product extractant.
    Type: Grant
    Filed: June 17, 2011
    Date of Patent: December 8, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Douglas Robert Anton, Jelena Cirakovic, Bruce A. Diner, Michael Charles Grady, Francis J. Woerner
  • Patent number: 9139820
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
    Type: Grant
    Filed: January 27, 2015
    Date of Patent: September 22, 2015
    Assignee: Codexis, Inc.
    Inventors: Emily Mundorff, Erik Jan De Vries
  • Patent number: 9040265
    Abstract: The invention relates to a process for the enantioselective enzymatic reduction of a keto compound to the corresponding chiral hydroxy compound, wherein the keto compound is reduced with an oxidoreductase in the presence of a cofactor, and is characterized in that an oxidoreductase is used which has an amino acid sequence in which (a) at least 70% of the amino acids are identical to the amino acids of one of the amino acid sequences SEQ ID No 1, SEQ ID No 6 and SEQ ID No 8, or (b) at least 55% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 2, or (c) at least 65% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 3, or (d) at least 75% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 4, or (e) at least 65% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 5, or (0 at least 50% of the amino acids are identical to the amino acids of the amino acid sequence
    Type: Grant
    Filed: October 25, 2011
    Date of Patent: May 26, 2015
    Assignee: IEP GMBH
    Inventors: Anke Tschentscher, Antje Gupta, Maria Bobkova
  • Publication number: 20150139977
    Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
    Type: Application
    Filed: October 22, 2014
    Publication date: May 21, 2015
    Inventors: David Weiner, David Blum, Alexander Varvak, Shaun Healey, Kristine Chang, Geoff Hazlewood, Thomas Todaro, Grace Desantis, Hwai Chang, Connie Jo Hansen, Scott W. Beaver, Thomas Woodward, Charles Hancock
  • Publication number: 20150140627
    Abstract: Provided is a method for producing bioproducts, including the steps of: culturing a first microorganism to produce bioalcohol; hydrolyzing the first microorganism; separating the bioalcohol; obtaining wastes from the hydrolyzed bioalcohol fermentation; and inoculating a second microorganism into the wastes from the hydrolyzed bioalcohol fermentation.
    Type: Application
    Filed: May 14, 2013
    Publication date: May 21, 2015
    Inventors: Hyo-Hak Song, Julia Lee, Gi-Wook Choi, Se-Kwon Moon
  • Publication number: 20150140606
    Abstract: A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (4): (1) subjecting a cellulose-containing biomass to a dilute sulfuric acid treatment and thereafter separating the treated cellulose-containing biomass into a dilute sulfuric acid-treated liquid and a cellulose-containing solid content; (2) adding a cellulase to the cellulose-containing solid content to hydrolyze the cellulose and thereafter obtaining a sugar liquid; (3) filtering the dilute sulfuric acid-treated liquid through a nanofiltration membrane at pH 2.5 or lower to thereby separate a sugar concentrated liquid as a retentate and at the same time recover a sulfuric acid aqueous solution as a permeate; and (4) reusing the whole amount or a part of the sulfuric acid aqueous solution obtained in (3) in the dilute sulfuric acid treatment in (1).
    Type: Application
    Filed: June 11, 2013
    Publication date: May 21, 2015
    Inventors: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada
  • Patent number: 9034613
    Abstract: A process for producing a compound which is (3R)-hydroxybutyl (3R)-hydroxybutyrate of formula (I) which process comprises submitting, to enantioselective reduction, a compound of the following formula (II), (III) or (IV).
    Type: Grant
    Filed: April 16, 2009
    Date of Patent: May 19, 2015
    Assignees: ISIS Innovation Limited, The United States of America, as Represented by the Secretary, Department of Health & Human Services
    Inventors: Jeremy Robertson, Kieran Clarke, Richard Lewis Veech
  • Publication number: 20150132806
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
    Type: Application
    Filed: January 27, 2015
    Publication date: May 14, 2015
    Inventors: Emily Mundorff, Erik Jan De Vries
  • Publication number: 20150125902
    Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Application
    Filed: August 24, 2012
    Publication date: May 7, 2015
    Applicant: Novozymes A/S
    Inventors: Tianqi Sun, Ming Li
  • Publication number: 20150125906
    Abstract: The present invention relates to methods of degrading or converting biomass material enriched with hemicellulosic material into fermentable sugars.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Prashant Iyer, Harry Showmaker, Hui Xu, Kishore Rane
  • Publication number: 20150125912
    Abstract: The invention provides a process for the preparation of hydrocarbons substituted with at least one group containing at least one oxygen atom, comprising the process steps A) reaction of a carbon source comprising at least one selected from CO2 and CO to give acetate and/or ethanol with a first microorganism, B) separating off of the acetate from the first microorganism, C) reaction of the acetate to give a hydrocarbon substituted with at least one group containing at least one oxygen atom with a second microorganism and optionally D) purification of the hydrocarbon substituted with at least one group containing at least one oxygen atom.
    Type: Application
    Filed: May 8, 2013
    Publication date: May 7, 2015
    Applicant: Evonik Industries AG
    Inventors: Thomas Haas, Eva Maria Wittmann
  • Patent number: 9023628
    Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.
    Type: Grant
    Filed: September 11, 2014
    Date of Patent: May 5, 2015
    Assignee: Xyleco, Inc.
    Inventor: Marshall Medoff
  • Publication number: 20150107318
    Abstract: The method contains the following steps. In step A, supersonic waves are applied to Mountain Litsea fruits for excitation and sterilization. In step B, the inferior fruits and the cleaning water are removed from the water tank, placed in a fermentative tank, and left for fermentation. After the fermentation, a pressing and separation process is conducted. In step C, the superior fruits are placed in a separate fermentative tank for fermentation into mofetil alcohol. In step D, the superior fruits are alternatively mixed with pure water, crushed, and placed in a boiler for aging and sterilization. Then the crushed and aged fruits are placed in yet another fermentative tank with pure water for fermentation. After the fermentation, a pomace separation process is conducted. The separated pomace becomes a Mountain Litsea wine lees, whereas the separated water becomes a Mountain Litsea enzymatic liquid.
    Type: Application
    Filed: October 22, 2013
    Publication date: April 23, 2015
    Inventors: HENG-JUI HSU, TSU-YU HSU
  • Patent number: 9005946
    Abstract: The invention provides variants of the Clostridium thermocellum endoglucanase (CelG) that have improved endoglucanase activity compared to the wild type enzyme. Also provided are related polynucleotides, compositions, vectors, host cells, and methods of use.
    Type: Grant
    Filed: March 10, 2010
    Date of Patent: April 14, 2015
    Assignee: Codexis, Inc.
    Inventors: Jie Yang, Xiyun Zhang, Ish Kumar Dhawan, Andrew Shaw
  • Publication number: 20150099276
    Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.
    Type: Application
    Filed: December 16, 2014
    Publication date: April 9, 2015
    Inventors: Dawid BRAT, Eckhard BOLES, Marco KELLER, Beate ROTHER
  • Patent number: 8999686
    Abstract: Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described.
    Type: Grant
    Filed: October 7, 2009
    Date of Patent: April 7, 2015
    Assignee: REG Life Sciences, LLC
    Inventors: Zhihao Hu, Vikranth Arlagadda
  • Publication number: 20150093797
    Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.
    Type: Application
    Filed: September 2, 2014
    Publication date: April 2, 2015
    Applicant: AMYRIS, INC.
    Inventors: Timothy Stevens GARDNER, Kristy Michelle HAWKINS, Adam Leon MEADOWS, Annie Ening TSONG, Yoseph TSEGAYE
  • Patent number: 8993296
    Abstract: An object of the present invention is to provide enzymes associated with equol synthesis, genes coding such enzymes, and a process for producing equol and its intermediates using the enzymes and genes. The present invention provides a dihydrodaidzein synthesizing enzyme, tetrahydrodaidzein synthesizing enzyme, equol synthesizing enzyme, and genes coding these enzymes. The present invention also provides a process for synthesizing dihydrodaidzein, tetrahydrodaidzein, and/or equol using these enzymes.
    Type: Grant
    Filed: January 15, 2014
    Date of Patent: March 31, 2015
    Assignee: Otsuka Pharmaceutical Co., Ltd.
    Inventors: Yoshikazu Shimada, Setsuko Yasuda, Masayuki Takahashi, Takashi Hayashi, Norihiro Miyazawa, Yasuhiro Abiru, Tadaaki Ohtani, Ikutaro Sato
  • Patent number: 8993269
    Abstract: Glucosyl stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The short-chain glucosyl stevia compositions were purified to >95% content of total steviol glycosides. The compositions can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.
    Type: Grant
    Filed: November 6, 2013
    Date of Patent: March 31, 2015
    Assignee: PureCircle USA Inc.
    Inventor: Avetik Markosyan
  • Patent number: 8993283
    Abstract: A production method for biomass-alcohol includes a saccharification step of saccharifying biomass, a first concentrating step of ultrasonically vibrating the saccharified solution and atomizing the saccharified solution into a mist, so as to elevate the sugar concentration in the saccharified solution by removing water from the saccharified solution, a fermentation step of fermenting the saccharified solution concentrated in the first concentrating step to form an alcohol water solution, and second concentrating step of separating alcohol from the alcohol water solution fermented in the fermentation step.
    Type: Grant
    Filed: March 31, 2009
    Date of Patent: March 31, 2015
    Assignees: Ultrasound Brewery, Mitsui Engineering & Shipbuilding Co., Ltd.
    Inventors: Kazuo Matsuura, Fusatsugu Abe, Tetsuo Fukazu, Takuji Cho, Kousuke Kimoto
  • Patent number: 8993282
    Abstract: The present invention provides a method for producing a carotenoid, which comprises culturing a carotenoid-producing bacterium in an amino acid-supplemented medium, and collecting the carotenoid from the resulting cultured product, wherein the amino acid is at least one selected, from the group consisting of glutamic acid, aspartic acid, glutamine, asparagine, alanine, glycine, serine, threonine, arginine, tyrosine, proline, phenylalanine and leucine, and salts thereof.
    Type: Grant
    Filed: October 16, 2009
    Date of Patent: March 31, 2015
    Assignee: JX Nippon Oil & Energy Corporation
    Inventors: Kazuaki Hirasawa, Akira Tsubokura, Hiroshi Satou, Tetsuhisa Yata
  • Publication number: 20150087031
    Abstract: The present invention relates to methods of processing lignocellulosic material to obtain hemicellulose sugars, cellulose sugars, lignin, cellulose and other high-value products. Also provided are hemicellulose sugars, cellulose sugars, lignin, cellulose, and other high-value products.
    Type: Application
    Filed: May 3, 2013
    Publication date: March 26, 2015
    Inventors: Robert Jansen, Claire Gregoire, philip Travisano, Lee Madsen, Neta Matis, Yael Har-Tal, Shay Eliahu, James Alan Lawson, Noa Lapidot, Luke Burke, Aharon M. Eyal, Timothy Allen Bauer, Hagit Sade, Paul Mcwilliams, Ziv-Vladimir Belman, Bassem Hallac, Michael Zviely, Yelena Gershinksy, Adam Carden
  • Patent number: 8986962
    Abstract: This invention provides co-cultures of photosynthetic microorganisms and biofuel producing microorganisms. In certain embodiments, polysaccharide-producing, photosynthetic microorganisms are microalgae having frustules provide a substrate on which biofuel-producing microorganisms can grow. In other embodiments, the photosynthetic microorganisms produce a lipid and the non-photosynthetic microorganisms produce a solvent in which the lipid is soluble.
    Type: Grant
    Filed: July 21, 2011
    Date of Patent: March 24, 2015
    Inventor: Pamela R. Contag
  • Patent number: 8986948
    Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.
    Type: Grant
    Filed: July 1, 2009
    Date of Patent: March 24, 2015
    Assignee: Lesaffre et Compagnie
    Inventors: Dawid Brat, Eckhard Boles, Marco Keller, Beate Wiedemann
  • Patent number: 8980578
    Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
    Type: Grant
    Filed: June 10, 2013
    Date of Patent: March 17, 2015
    Assignee: Codexis, Inc.
    Inventors: Ryan Fong, Xiyun Zhang, Chris Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
  • Patent number: 8980595
    Abstract: The present invention describes a process for the production of alcohol, known as second generation production, from a lignocellulosic substrate, in which a controlled quantity of furfural is produced in situ and which comprises at least the following steps: a first step for alkaline chemical pre-treatment of the substrate; a step for adjusting the pH of the substrate; then a step for enzymatic hydrolysis of said pre-treated substrate; a step for alcoholic fermentation of the hydrolysate obtained with an alcohol-producing microorganism; a step for separation/purification; and a step for acid conversion of at least one liquid fraction into furfural, said liquid fraction being obtained during one of the preceding steps; a step for mixing said furfural obtained with the pre-treated substrate during the pH adjusting step.
    Type: Grant
    Filed: May 7, 2010
    Date of Patent: March 17, 2015
    Assignee: IFP Energies Nouvelles
    Inventors: Marcel Ropars, Caroline Aymard, Flora Ghisoni, Sandra Menir
  • Patent number: 8980599
    Abstract: A process for the production of glucose from a pretreated lignocellulosic feedstock is provided. The method comprises enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting the hydrolyzate slurry in a fermentation reaction to produce a fermentation broth comprising alcohol. A process stream is obtained comprising unhydrolyzed cellulose, which is then subjected to a denaturing step, preferably comprising exposing the unhydrolyzed cellulose to elevated temperatures, thereby producing a heat-treated stream comprising the unhydrolyzed cellulose. The heat-treated stream comprising unhydrolyzed cellulose is then further hydrolyzed with cellulase enzymes to hydrolyze the cellulose to glucose.
    Type: Grant
    Filed: July 29, 2008
    Date of Patent: March 17, 2015
    Assignee: Iogen Energy Corporation
    Inventors: Jeffrey S. Tolan, Brian Foody, Stephen Rowland
  • Patent number: 8980587
    Abstract: A bacterium belonging to the genus Streptomyces having an ability to produce reveromycin A or a synthetic intermediate thereof, the bacterium being modified so as to increase expression of revQ gene coding for the amino acid sequence of SEQ ID NO: 36 or an amino acid sequence having an identity of not less than 80% to SEQ ID NO: 36 as compared with a parent strain, thereby the above-mentioned production ability is increased as compared with the parent strain.
    Type: Grant
    Filed: August 30, 2011
    Date of Patent: March 17, 2015
    Assignee: RIKEN
    Inventors: Hiroyuki Osada, Shunji Takahashi, Makoto Kawatani, Yoshiyuki Sakaki, Atsushi Toyoda
  • Patent number: 8980598
    Abstract: A dry solids staging fermentation process for producing an end-product, such as ethanol is disclosed said process including an initial fermentation step including combining a first fermentable substrate with one or more starch hydrolyzing enzymes and fermenting organisms in a fermentation vessel and a loading step which includes adding a second fermentable substrate to the fermentation vessel wherein the percent dry solids (% DS) of the fermentation broth increases over time.
    Type: Grant
    Filed: June 14, 2005
    Date of Patent: March 17, 2015
    Assignee: Danisco US Inc.
    Inventors: Gopal Chotani, Gang Duan, Craig E. Pilgrim, Jayarama K. Shetty
  • Publication number: 20150057461
    Abstract: The present invention relates to a transformant which is transformed to express Baeyer-Villiger monooxygenase (BVMO), a method for producing C5-C14 medium-chain ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids, or alcohols from C16-C20 long-chain fatty acids by biotransformation using the transformant, a method for producing a fatty acid derivative having an ester group which is introduced into the chain thereof from keto fatty acid using the BVMO, and novel ?-hydroxy fatty acids which are prepared by the method. Degradation products such as C5 to C14 ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids, alcohols can be produced in a large amount from C16 to C20 long-chain fatty acids contained in a medium by biotransformation using a transformant capable of expressing BVMO of the present invention. Therefore, it can be widely used to produce ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids or alcohols in a more safe and economic manner.
    Type: Application
    Filed: April 5, 2013
    Publication date: February 26, 2015
    Inventors: Jin Byung Park, Ji Won Song, Eun Yeong Jeon
  • Publication number: 20150059018
    Abstract: The present invention relates to nucleic acids sequences derived from Valeriana officinalis and/or Persicaria hydropiper and encoding drimenol synthase polypeptides. The present invention also provides the amino acid sequences of the polypeptides. The invention further provides host cells or organisms genetically modified to harbour the polynucleotides of the invention. A method to produce drimenol and/or a drimenol derivative by contacting farnesyl diphosphate with a polypeptide having a drimenol synthase activity is also part of this invention.
    Type: Application
    Filed: October 19, 2012
    Publication date: February 26, 2015
    Inventors: Hendrik Jan Bouwmeester, Maurice Gerard Leon Henquet, Maarten Anthonie Jongsma
  • Publication number: 20150056671
    Abstract: Provided is a method of producing a fatty acid ester in a high yield through a simple operation using Euglena as a material. The method of producing a fatty acid ester comprises the following steps (a) and (b): (a) adding 0.001 to 9.5 [PU/g-dry cell] of at least one kind of protease to Euglena to react the Euglena and the protease in an aqueous phase; and (b) performing phase separation and collection of a fatty acid ester from a reaction liquid of the step (a).
    Type: Application
    Filed: April 1, 2013
    Publication date: February 26, 2015
    Inventors: Saki Hamada, Hiroyuki Konishi, Takaaki Watanabe
  • Patent number: 8962287
    Abstract: An object of the present invention is to provide a cell capable of efficiently producing scyllo-inositol from myo-inositol and a simple method of manufacturing scyllo-inositol using the cell. The above-mentioned object is achieved by a Bacillus subtilis cell, in which a function of a protein having a scyllo-inositol dehydrogenase activity is lost, and the manufacture of scyllo-inositol using the cell, based on a novel finding that the protein having a scyllo-inositol dehydrogenase activity and a protein having a 2-keto-myo-inositol ketoreductase function are present in Bacillus subtilis.
    Type: Grant
    Filed: October 30, 2009
    Date of Patent: February 24, 2015
    Assignee: National University Corporation KOBE University
    Inventors: Kenichi Yoshida, Hitoshi Ashida
  • Publication number: 20150050691
    Abstract: The present invention relates to a method for preparing a mutant E. coli strain, capable of simultaneously using glucose and xylose, by genetic engineering and evolutionary adaptation; the mutant E. coli prepared using the same; and a method for producing biofuels, biologically active ingredients, medicinal materials or base chemicals for the chemical industry using the mutant E. coli. Being capable of simultaneously using glucose and xylose, in contrast to wild-type E. coli, the mutant E. coli can be effectively applied to the enzymatic saccharification process of producing biofuels from a biomass.
    Type: Application
    Filed: July 2, 2014
    Publication date: February 19, 2015
    Applicant: UNIST ACADEMY-INDUSTRY RESEARCH CORPORATION
    Inventors: Sung Kuk LEE, Goo Hee KIM, Seong Hun JEONG, Suk Min KIM, Bae Young CHOI