Attached To The Carrier Via A Bridging Agent Patents (Class 435/181)
  • Patent number: 10246677
    Abstract: One embodiment provides a commensal gut production platform for ex vivo production of human gut commensal microbiota. Another embodiment provides devices, systems and methods for ex vivo culturing of gut microflora in a system that mimics the human gut environment. The culturing of the commensal microbiota in the disclosed systems produces gut microbiota having defined characteristics and properties that can be exploited to treat various conditions in a subject.
    Type: Grant
    Filed: February 9, 2018
    Date of Patent: April 2, 2019
    Inventor: Bobban Subhadra
  • Patent number: 10059979
    Abstract: Spherical, magnetizable polyvinyl alcohol microparticles, methods for their production, and use thereof are provided in the invention. The microparticles are especially useful for diagnostic purposes. The method enables the production of microparticles having a particle size distribution in the range of 0.5 to 3 ?m, and includes the following steps, dispersing a nanoparticulate, magnetizable material in an aqueous phase which contains polyvinyl alcohol in dissolved form, adding the aqueous phase to an organic phase, immiscible with said aqueous phase and containing at least one emulsifier, producing an emulsion by stirring at a temperature of 25° C. or higher, and adding at least one water-soluble crosslinking agent while stirring is continued.
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: August 28, 2018
    Assignee: PerkinElmer chemagen Technologie GmbH
    Inventors: Jürgen Oster, Thomas Sommer, Lothar À Brassard
  • Patent number: 10050296
    Abstract: A biofuel cell comprising an anode and cathode wherein biocatalytic enzymes are purposefully oriented at each side of the fuel cell so as to increase and/or optimize the enzymes' performance in catalysis and/or electron transfer.
    Type: Grant
    Filed: January 13, 2014
    Date of Patent: August 14, 2018
    Assignee: STC.UNM
    Inventors: Sofiya Malinova Babanova, Plamen B Atanassov
  • Patent number: 9795683
    Abstract: A water soluble polymer, in particular polysialic acid (PSA) or a modified PSA (mPSA), is conjugated to an oxidized carbohydrate moiety of a glycoprotein other than a blood coagulation protein or to a ganglioside or drug delivery system by contacting the oxidized carbohydrate moiety with the water soluble polymer, wherein said water soluble polymer contains an aminooxy group and an oxime linkage is formed between the oxidized carbohydrate moiety and the aminooxy group on the water soluble polymer or wherein said water soluble polymer contains a hydrazide group and a hydrazone linkage is formed between the oxidized carbohydrate moiety and the hydrazide group on the water soluble polymer. Conjugates of aminooxy- or hydrazide-water soluble polymer, such as PSA and mPSA, are thus obtained in which the PSA or mPSA is attached via a carbohydrate moiety.
    Type: Grant
    Filed: July 26, 2010
    Date of Patent: October 24, 2017
    Assignees: Lipoxen Technologies Limited, Baxalta Incorporated, Baxalta GmbH
    Inventors: Sanjay Jain, Gregory Gregoriadis, Archana Dwivedi, Srijit Nath, Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Peter Turecek
  • Patent number: 9757469
    Abstract: Polyethylene glycol modified human chorionic gonadotropin (hCG), preparations thereof, compositions thereof, and methods of preparation and use thereof are described.
    Type: Grant
    Filed: March 29, 2012
    Date of Patent: September 12, 2017
    Assignee: Ferring B.V.
    Inventors: Ian Cottingham, Daniel Plaksin, Jenny Aharanov
  • Patent number: 9659870
    Abstract: Wiring comprises a multilayer graphene including graphene sheets, an interlayer substance disposed between layers of the multilayer graphene, and an organic compound layer connected to a side surface of the multilayer graphene. The organic compound layer contains a photoisomerizable organic group connected to the multilayer graphene.
    Type: Grant
    Filed: September 1, 2015
    Date of Patent: May 23, 2017
    Assignee: Kabushiki Kaisha Toshiba
    Inventors: Hisao Miyazaki, Tadashi Sakai
  • Patent number: 9540493
    Abstract: The present invention aims to provide a method for surface-modifying a rubber vulcanizate or a thermoplastic elastomer, which is capable of cost-effectively imparting a variety of functions, such as sliding properties and biocompatibility, according to the applications. The present invention relates to a surface modification method for surface-modifying an object of a rubber vulcanizate or a thermoplastic elastomer, the method including: step 1 of forming polymerization initiation points A on a surface of the object; step 2 of radically polymerizing a non-functional monomer, starting from the polymerization initiation points A, to grow non-functional polymer chains; step 3 of forming polymerization initiation points B on the surface of the object where the non-functional polymer chains are formed; and step 4 of radically polymerizing a functional monomer, starting from the polymerization initiation points B, to grow functional polymer chains.
    Type: Grant
    Filed: December 16, 2013
    Date of Patent: January 10, 2017
    Assignee: SUMITOMO RUBBER INDUSTRIES, LTD.
    Inventor: Yasuhisa Minagawa
  • Patent number: 9399085
    Abstract: The present invention is directed to immobilized biologically active entities that retain significant biological activity following mechanical manipulation of a substrate material to which the entities are immobilized.
    Type: Grant
    Filed: March 24, 2009
    Date of Patent: July 26, 2016
    Assignee: W. L. Gore & Associates, Inc.
    Inventors: Robert L. Cleek, Michael D. Daly, Krzysztof R. Pietrzak
  • Patent number: 9375515
    Abstract: The present invention is directed to immobilized biologically active entities that retain significant biological activity following mechanical manipulation of a substrate material to which the entities are immobilized.
    Type: Grant
    Filed: March 24, 2009
    Date of Patent: June 28, 2016
    Assignee: W. L. Gore & Associates, Inc.
    Inventors: Robert L. Cleek, Michael D. Daly, Krzysztof R. Pietrzak
  • Patent number: 9175061
    Abstract: Reductive amination of peptide-derived aldehydes with anilines or heteroarylamines containing a property-modifying group provides new, hydrolytically stable protein conjugates, suitable for therapy.
    Type: Grant
    Filed: July 5, 2007
    Date of Patent: November 3, 2015
    Assignee: Novo Nordisk Health Care AG
    Inventor: Florencio Zaragoza Dörwald
  • Patent number: 9080166
    Abstract: Disclosed is a composite of enzyme and fiber matrix with three-dimensional structure. The composite of enzyme and fiber matrix with three-dimensional structure includes a significantly large amount of an enzyme loaded in and immobilized in/onto a matrix when compared to conventional composites. In addition, the immobilized enzyme is prevented from leaching from the matrix when an external impact is applied to the composite of enzyme and fiber matrix with three-dimensional structure. Therefore, the stability of the composite of enzyme and fiber matrix with three-dimensional structure of the present invention is maintained even after a long period passes since a remarkably great amount of enzymes compared with a known composite can be supported and immobilized to a matrix, and the immobilized enzyme is not easily released by an external impact.
    Type: Grant
    Filed: April 19, 2011
    Date of Patent: July 14, 2015
    Assignee: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION
    Inventors: Jungbae Kim, Hyeong-Seok Kim
  • Patent number: 8945896
    Abstract: Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed.
    Type: Grant
    Filed: January 23, 2013
    Date of Patent: February 3, 2015
    Assignee: 3M Innovative Properties Company
    Inventors: Jerald K. Rasmussen, Catherine A. Bothof, Kannan Seshadri, Erin A. Satterwhite, Robert T. Fitzsimons, Jr., James L. Hembre, Mahfuza B. Ali
  • Publication number: 20150017246
    Abstract: The invention provides novel chemical entities based on sugar alcohols. These new chemical entities are biocompatible and biodegradable. The molecules can be made in a single and pure form. The molecular weights of these molecules range from small (<1000 Da) to large (1000-120,000 Da). The sugar alcohol-based molecules can have functional groups throughout the molecule for crosslinking compounds, such as the preparation of antibody-drug conjugates, or to facilitate the delivery of therapeutic proteins, peptides, siRNA, and chemotherapeutic drugs. Also provided are new conjugate entities prepared through sugar alcohol molecules. Methods of synthesizing sugar alcohol-based molecules and conjugates are also within the scope of the invention.
    Type: Application
    Filed: January 15, 2014
    Publication date: January 15, 2015
    Applicant: CellMosaic, Inc.
    Inventor: Yumei Huang
  • Patent number: 8911986
    Abstract: Temporary active coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
    Type: Grant
    Filed: April 29, 2011
    Date of Patent: December 16, 2014
    Assignees: Toyota Motor Engineering & Manufacturing North America, Inc., Toyota Motor Corporation
    Inventors: Hongfei Jia, Songtao Wu, Masahiko Ishii, Minjuan Zhang
  • Patent number: 8871483
    Abstract: The present disclosure provides multi-walled carbon nanotubes and carbon nanotubes (CNTs) displaying catalytic enzymes bound to the nanotube sidewalls and devices, such as electrodes, incorporating these catalytic enzyme-bound CNTs (cebCNTs).
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: October 28, 2014
    Assignee: STC.UNM
    Inventors: Plamen B Atanassov, Claudia W Narvaez Villarrubia, Sofiya Malinova Babanova
  • Patent number: 8835144
    Abstract: Dual-functional nonfouling surfaces and materials, methods for making dual-functional nonfouling surfaces and materials, and devices that include dual-functional nonfouling surfaces and materials. The dual-functional surfaces are nonfouling surfaces that resist non-specific protein adsorption and cell adhesion. The dual-functional surfaces and materials include covalently coupled biomolecules (e.g., target binding partners) that impart specific biological activity thereto. The surfaces and materials are useful in medical diagnostics, biomaterials and bioprocessing, tissue engineering, and drug delivery.
    Type: Grant
    Filed: June 29, 2009
    Date of Patent: September 16, 2014
    Assignee: University of Washington
    Inventors: Shaoyi Jiang, Zheng Zhang, Shengfu Chen, Hana Vaisocherova
  • Patent number: 8802410
    Abstract: There is disclosed an analytical method and a sensor suitable for carrying out the method. More specifically, there is disclosed a method for preparing a mass sensitive chemical sensor capable of detecting binding analyte species to a surface comprising cells.
    Type: Grant
    Filed: March 20, 2010
    Date of Patent: August 12, 2014
    Assignee: Attana AB
    Inventors: Camilla Käck, Julien Saint-Guirons, Björn Ingemarsson, Kerstin Lundell
  • Patent number: 8796009
    Abstract: A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.
    Type: Grant
    Filed: June 21, 2010
    Date of Patent: August 5, 2014
    Assignees: Toyota Motor Engineering & Manufacturing North America, Inc., Toyota Motor Corporation
    Inventors: Hongfei Jia, Wei Song, Masahiko Ishii, Minjuan Zhang
  • Patent number: 8765393
    Abstract: An affinity ligand-matrix conjugate of the structure Z-Spacer-[NX-A]m-NY-A-NK2 is useful for the isolation, separation, purification, characterization, identification or quantification of endotoxins in an aqueous system, wherein m is an integer of at least one; each A independently represents an optionally substituted linear, branched or cyclic saturated hydrocarbon chain containing 1 to 6 carbon atoms; each X independently represents hydrogen or alkyl; Y is X or A-NX2; and Z is a support matrix attached to the ligand through an optional spacer arm (Spacer).
    Type: Grant
    Filed: May 22, 2003
    Date of Patent: July 1, 2014
    Assignee: Prometic Biosciences Ltd
    Inventors: Steven James Burton, Tadeusz Podgorski, Saji S. Eapen
  • Publication number: 20140170727
    Abstract: Separation media comprising a support and crosslinked whole cells fixed to the support. The separation media are useful in affinity columns for the separation of the antibodies from a solution, particularly for the separation of polyclonal antibodies that have been raised against the same type of whole cells. In an embodiment, the whole cells are whole bacterial cells.
    Type: Application
    Filed: December 18, 2013
    Publication date: June 19, 2014
    Applicant: NANOMR, INC.
    Inventors: Sergey A. Dryga, Robert Nadeau, Douglas Standridge
  • Patent number: 8697111
    Abstract: An osteochondral plug includes a first scaffold and a second scaffold. The first scaffold may be a solid scaffold containing one or more pendant reactive functional groups. The second scaffold capable of reacting with the one or more pendant reactive functional groups of the first scaffold.
    Type: Grant
    Filed: May 12, 2010
    Date of Patent: April 15, 2014
    Assignee: Covidien LP
    Inventors: Arpan Desai, Timothy Sargeant, Atu Agawu, Joshua Stopek
  • Patent number: 8679807
    Abstract: There is provided a method for covalent immobilization of at least one molecule comprising at least one amino group, said method comprising the sequential steps of: a) providing a surface comprising —SH groups, b) oxidizing the surface comprising —SH groups using redox reactions in the presence of noble metal ions, and c) contacting the surface with at least one molecule comprising at least one amino group to obtain a covalent binding of the at least one molecule to the surface, wherein said at least one amino group is involved in obtaining said covalent bond. The immobilized molecules are immobilized via stable covalent bonds. The method is more versatile since it can be performed as a one step method. All reaction steps are performed in aqueous solution. All steps can be performed at room temperature. The chemicals used are less expensive and less toxic compared to the prior art.
    Type: Grant
    Filed: July 27, 2011
    Date of Patent: March 25, 2014
    Assignee: Lab-On-A-Bead
    Inventors: Sven Oscarsson, Kristofer Eriksson, Leif Nyholm
  • Patent number: 8642744
    Abstract: Crosslinking reagents and methods for using the same for analysis of protein-protein interactions, are provided. The crosslinking reagents include a trifunctional scaffold that links two protein linking groups to each other and branches to link an affinity tag, where the protein linking groups can be fragmented from the scaffold. The distance between the two protein linking groups can be selected to crosslink two proteins of a protein complex via accessible amino acid residues. Also provided are crosslinked polypeptide compounds and kits that include crosslinking reagents. These reagents and methods find use in a variety of applications in which crosslinking of proteins in desired.
    Type: Grant
    Filed: October 28, 2010
    Date of Patent: February 4, 2014
    Assignee: Agilent Technologies, Inc.
    Inventors: Brian Phillip Smart, James Alexander Apffel, Jr.
  • Patent number: 8642308
    Abstract: The present disclosure provides multi-walled carbon nanotubes and carbon nanotubes (CNTs) displaying catalytic enzymes bound to the nanotube sidewalls and devices, such as electrodes, incorporating these catalytic enzyme-bound CNTs (cebCNTs).
    Type: Grant
    Filed: July 7, 2011
    Date of Patent: February 4, 2014
    Assignee: STC.UNM
    Inventors: Plamen Atanassov, Dmitri Ivnitski, Ramaraja P. Ramasamy, Heather R. Luckarift, Glenn R. Johnson, Carolin Lau
  • Publication number: 20140004158
    Abstract: Cell attachment coatings for articles such as implantable medical devices and cell culture vessels are disclosed. The coatings include an intermediate coater layer which includes a phosphorous-containing component that is bonded in the coating by reacted photoreactive functional groups. The coating also include a second coated layer including an immobilized ECM protein or peptide that includes an active portion of an ECM protein that is able to serve as an outer layer to contact cells during use. The coatings promoted enhanced cell binding and growth.
    Type: Application
    Filed: June 28, 2013
    Publication date: January 2, 2014
    Inventor: Joseph S. McGonigle
  • Patent number: 8580545
    Abstract: A method and a composition for delivery of a biomaterial to an animal cell or a tissue, the composition includes (a) a biomaterial; (b) a biodegradable cross-linker portion having a hydrolyzable bond, wherein the biodegradable cross-linker portion is covalently bound to the biomaterial; and (c) a substrate, wherein the substrate is covalently bound to the biodegradable cross-linker portion, provided that the biodegradable cross-linker is adapted to hydrolyze by breaking the hydrolyzable bond and thereby release and deliver the biomaterial. A process of making the composition is also provided.
    Type: Grant
    Filed: November 29, 2011
    Date of Patent: November 12, 2013
    Assignee: The Children's Hospital of Philadelphia
    Inventors: Ivan Alferiev, Robert J. Levy, Ilia Fishbein
  • Patent number: 8563245
    Abstract: A method that modifies surface properties of a substrate by manipulating the immobilized biomolecules in mild biological condition. The manipulation comprised steps of: providing a biomolecule combined with at least one ssDNA combined with a first protein through an affinity binding tag; adding a second ssDNA conjugated with a second protein with a concentration greater than that of the first protein; and replacing the first protein on the ssDNA with the second protein through chemical competitive principle. The invention may comprise the steps with proper design of biotinylated DNA probes, the functionalized ssDNA nanotemplates can be recovered to its unbound state through a thermodynamic principle.
    Type: Grant
    Filed: April 2, 2012
    Date of Patent: October 22, 2013
    Assignee: National Applied Research Laboratories
    Inventors: Ming-Yu Liu, Yuh-Shyong Yang, Yen-Pei Lu, Chih-Heng Lin
  • Patent number: 8546493
    Abstract: Multi-armed, monofunctional, and hydrolytically stable polymers are described having the structure wherein Z is a moiety that can be activated for attachment to biologically active molecules such as proteins and wherein P and Q represent linkage fragments that join polymer arms polya and polyb, respectively, to central carbon atom, C, by hydrolytically stable linkages in the absence of aromatic rings in the linkage fragments. R typically is hydrogen or methyl, but can be a linkage fragment that includes another polymer arm. A specific example is an mPEG disubstituted lysine having the structure where mPEGa and mPEGb have the structure CH3O—(CH2CH2O)nCH2CH2— where n may be the same or different for polya- and polyb- and can be from 1 to about 1,150 to provide molecular weights of from about 100 to 100,000.
    Type: Grant
    Filed: December 14, 2012
    Date of Patent: October 1, 2013
    Assignee: Nektar Therapeutics
    Inventors: J. Milton Harris, Francesco M. Veronese, Paolo Caliceti, Oddone Schiavon
  • Patent number: 8530212
    Abstract: A process for producing a substrate having an adhesive surface, which process comprises: (a) contacting the substrate with a carbene precursor, which carbene precursor is a compound of the following formula (1): whose substituent groups are defined herein, provided that when R is aryl or heteroaryl, said aryl or heteroaryl may be substituted by one, two, three, four or five groups, which groups are independently selected from various groups including -LB-WB; and (b) either: (i) when WA or WB comprises an adhesive functional group, generating a carbene reactive intermediate from the carbene precursor so that it reacts with the substrate to functionalise the surface, thereby yielding said substrate having an adhesive surface; or (ii) when WA or WB comprises a group which is a precursor of an adhesive functional group, generating a carbene reactive intermediate from the carbene precursor so that it reacts with the substrate to functionalise the surface, and (c) converting said group which is a precursor into an
    Type: Grant
    Filed: August 22, 2007
    Date of Patent: September 10, 2013
    Assignee: ISIS Innovation Limited
    Inventors: Mark Moloney, Jon-Paul Griffiths
  • Publication number: 20130230896
    Abstract: A composition of enzyme, polymer, and crosslinker forms a network of covalently bound macromolecules. The covalently immobilized enzyme preparation has enzymatic activity, and retains stable activity when dried and stored at ambient conditions. Methods for preparing an immobilized enzyme and methods for using the enzyme are disclosed.
    Type: Application
    Filed: March 18, 2013
    Publication date: September 5, 2013
    Applicant: FRESENIUS MEDICAL CARE HOLDINGS, INC.
    Inventor: Stephen A. Merchant
  • Patent number: 8512728
    Abstract: A method for in situ formation of a medical device on biological tissue includes attaching a plurality of reactive members of a primary specific binding pair to a surface of the biological tissue, and providing a plurality of fibers having attached thereto a plurality of complementary reactive members of the primary specific binding pair, wherein upon contact of the reactive members on the surface of the biological tissue with the complimentary reactive members on the fibers, covalent bonds are formed between the reactive members and the complementary reactive members, thus adhering the fibers to the tissue. The fibers can incorporate functionalities which may cause them to bind to one another.
    Type: Grant
    Filed: February 19, 2010
    Date of Patent: August 20, 2013
    Assignee: Sofradim Production
    Inventors: Sebastien Ladet, Philippe Gravagna
  • Publication number: 20130210111
    Abstract: A substrate having compounds disposed thereon for immobilizing a functional molecule, each compound having a chain including: a moiety R that is chemically coupled to the substrate, the moiety R being selected from the group consisting of an ether, ester, carbonyl, carbonate ester, thioether, disulfide, sulfinyl, sulfonyl, and carbonothioyl; and an epoxide-containing moiety that is coupled to the moiety R by a linker including at least one nucleophilic group.
    Type: Application
    Filed: March 15, 2013
    Publication date: August 15, 2013
    Applicant: TEMASEK POLYTECHNIC
    Inventors: Christian Gert BLUCHEL, Yanmei WANG
  • Patent number: 8507256
    Abstract: Sorption reinforced catalytic coating system for the degradation of threat agents including a synzyme coating about a material, the synzyme coating having bucket-shaped molecules for the sorption and degradation of the threat agents. A binding agent is configured for synzyme immobilization to maximize loading and retention of the synzyme coating on the material.
    Type: Grant
    Filed: June 17, 2010
    Date of Patent: August 13, 2013
    Assignee: Foster-Miller, Inc.
    Inventors: Yongwoo Lee, Tomasz Modzelewski, John P. Puglia, Steven E. Weiss, Robert A. Reinstein
  • Patent number: 8507255
    Abstract: Sorption reinforced catalytic coating system for the degradation of threat agents including a synzyme coating about a material configured for the degradation of the threat agents, the synzyme coating including bucket-shaped molecules configured for the sorption of the threat agents A binding agent is configured for synzyme immobilization to maximize loading and retention of the synzyme coating on the material.
    Type: Grant
    Filed: June 17, 2010
    Date of Patent: August 13, 2013
    Assignee: Foster-Miller, Inc.
    Inventors: Yongwoo Lee, Tomasz Modzelewski, John P. Puglia, Steven E. Weiss, Robert A. Reinstein
  • Publication number: 20130195752
    Abstract: Certain embodiments of the present invention provide functionalized nanoparticles and methods of use thereof. Certain embodiments provide nanoparticles functionalized with streptokinase. Certain embodiments of the present invention provide methods for treating a pathological fibrin associated disorder (e.g., cancer) in an animal.
    Type: Application
    Filed: January 31, 2013
    Publication date: August 1, 2013
    Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA
    Inventor: REGENTS OF THE UNIVERSITY OF MINNESOTA
  • Patent number: 8497357
    Abstract: A heterobifunctional poly(ethylene glycol) is provided having a hydrolytically degradable linkage, a first terminus comprising an acrylate group, and a second terminus comprising a target such as a protein or pharmaceutical agent or a reactive moiety capable of coupling to a target. Hydrogels can be prepared. The hydrogels can be used as a carrier for a protein or a pharmaceutical agent that can be readily released in a controlled fashion.
    Type: Grant
    Filed: April 13, 2012
    Date of Patent: July 30, 2013
    Assignee: Nektar Therapeutics
    Inventors: J. Milton Harris, Xuan Zhao
  • Publication number: 20130183702
    Abstract: Supramolecular assemblies for delivering active agents to cancerous or precancerous tissues in a subject are provided. These supramolecular assemblies are also useful in assays for detecting and imaging of cancerous and precancerous cells. The assemblies are protease-sensitive and comprise a peptide linkage containing a protease consensus sequence. The assemblies can be selectively targeted to cancerous tissue where the protease enzymes degrade the peptide linkage thereby releasing the active agents which were physically or mechanically contained in or retained by the supramolecular assembly.
    Type: Application
    Filed: March 13, 2013
    Publication date: July 18, 2013
    Applicant: Kansas State University Research Foundation
    Inventor: Kansas State University Research Foundation
  • Publication number: 20130149730
    Abstract: An emulsion-derived particle comprises a lattice of polymeric strands cross-linked by means of a cross-linking agent, and interstitial openings adjacent and around the strands. Functional groups are provided on the lattice and proteins and/or modified proteins can react with these, thereby to be bonded to the lattice and hence immobilised.
    Type: Application
    Filed: February 11, 2013
    Publication date: June 13, 2013
    Applicant: CSIR
    Inventor: CSIR
  • Patent number: 8440441
    Abstract: A substrate having compounds disposed thereon for immobilizing a functional molecule, each compound having a chain comprising: a moiety R that is chemically coupled to the substrate, said moiety R being selected from the group consisting of an ether, ester, carbonyl, carbonate ester, thioether, disulfide, sulfinyl, sulfonyl, and carbonothioyl; and an epoxide-containing moiety that is coupled to the moiety R by a linker comprising at least one nucleophilic group. Methods of preparing the substrate and use of the substrate are also disclosed.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: May 14, 2013
    Assignee: Temasek Polytechnic
    Inventors: Christian Bluchel, Yanmei Wang
  • Patent number: 8440816
    Abstract: The present invention is directed to branched reactive water-soluble polymers comprising at least two polymer arms, such as poly(ethylene glycol), attached to a central aliphatic hydrocarbon core molecule through ether linkages. The branched polymers bear at least one functional group for reacting with a biologically active agent to form a biologically active conjugate. The functional group of the branched polymer can be directly attached to the aliphatic hydrocarbon core or via an intervening linkage, such as a heteroatom, -alkylene-, —O-alkylene-O—, -alkylene-O-alkylene-, -aryl-O—, —O-aryl-, (—O-alkylene-)m, or (-alkylene-O—)m linkage, wherein m is 1-10.
    Type: Grant
    Filed: August 24, 2012
    Date of Patent: May 14, 2013
    Assignee: Nektar Therapeutics
    Inventors: Michael David Bentley, Xuan Zhao, Xiaoming Shen, William Dudley Battle, III
  • Patent number: 8394951
    Abstract: This disclosure concerns compounds which are useful as inhibitors of protein kinase C (PKC) and are thus useful for treating a variety of diseases and disorders that are mediated or sustained through the activity of PKC. This disclosure also relates to pharmaceutical compositions comprising these compounds, methods of using these compounds in the treatment of various diseases and disorders, processes for preparing these compounds and intermediates useful in these processes.
    Type: Grant
    Filed: January 13, 2010
    Date of Patent: March 12, 2013
    Assignee: Rigel Pharmaceuticals Inc.
    Inventors: Haoran Zhao, Rao Kolluri, Carlos Valdez, Kin Tso, Rajinder Singh, John Ramphal
  • Publication number: 20130048559
    Abstract: A substrate having compounds disposed thereon for immobilizing a functional molecule, each compound having a chain comprising: a moiety R that is chemically coupled to the substrate, said moiety R being selected from the group consisting of an ether, ester, carbonyl, carbonate ester, thioether, disulfide, sulfinyl, sulfonyl, and carbonothioyl; and an epoxide-containing moiety that is coupled to the moiety R by a linker comprising at least one nucleophilic group. Methods of preparing the substrate and use of the substrate are also disclosed.
    Type: Application
    Filed: February 18, 2011
    Publication date: February 28, 2013
    Applicant: TEMASEK POLYTECHNIC
    Inventors: Christian Bluchel, Yanmei Wang
  • Patent number: 8383380
    Abstract: Segmented water soluble polymers, containing a higher molecular weight segment linked to a lower molecular weight segment, are described. In one embodiment, the polymer segments are poly(ethylene glycol) segments. The segmented polymers are functionalized and are useful for conjugation to various moieties such as pharmacologically active substances. Also described are conjugates of such polymers and methods of their preparation.
    Type: Grant
    Filed: October 6, 2011
    Date of Patent: February 26, 2013
    Assignee: Nektar Therapeutics
    Inventors: Antoni Kozlowski, Xiaoming Shen, Michael D. Bentley, Zhihao Fang, Tony L. Sander
  • Patent number: 8354477
    Abstract: Multi-armed, monofunctional, and hydrolytically stable polymers are described having the structure wherein Z is a moiety that can be activated for attachment to biologically active molecules such as proteins and wherein P and Q represent linkage fragments that join polymer arms polya and polyb, respectively, to central carbon atom, C, by hydrolytically stable linkages in the absence of aromatic rings in the linkage fragments. R typically is hydrogen or methyl, but can be a linkage fragment that includes another polymer arm. A specific example is an mPEG disubstituted lysine having the structure where mPEGa and mPEGb have the structure CH3O—(CH2CH2O)nCH2CH2— wherein n may be the same or different for polya- and polyb- and can be from 1 to about 1,150 to provide molecular weights of from about 100 to 100,000.
    Type: Grant
    Filed: August 3, 2010
    Date of Patent: January 15, 2013
    Assignee: Nektar Therapeutics
    Inventors: J. Milton Harris, Francesco Maria Veronese, Paolo Caliceti, Oddone Schiavon
  • Publication number: 20120322134
    Abstract: The present invention is directed to branched reactive water-soluble polymers comprising at least two polymer arms, such as poly(ethylene glycol), attached to a central aliphatic hydrocarbon core molecule through ether linkages. The branched polymers bear at least one functional group for reacting with a biologically active agent to form a biologically active conjugate. The functional group of the branched polymer can be directly attached to the aliphatic hydrocarbon core or via an intervening linkage, such as a heteroatom, -alkylene-, —O-alkylene-O—, -alkylene-O-alkylene-, -aryl-O—, —O-aryl-, (—O-alkylene-)m, or (-alkylene-O—)m linkage, wherein m is 1-10.
    Type: Application
    Filed: August 24, 2012
    Publication date: December 20, 2012
    Applicant: Nektar Therapeutics
    Inventors: Michael David Bentley, Xuan Zhao, Xiaoming Shen, William Dudley Battle, III
  • Publication number: 20120208256
    Abstract: An immobilized enzyme comprises a solid support, an enzyme linked to the solid support and a spacer for coupling the enzyme to the solid support. A method for the production of immobilized enzymes comprises: providing a solid support having amino-functional groups coupled to the support surface; covalently coupling the amino-functional groups with a thermally labile radical initiator; and, contacting the support surface with a solution of polymerizable monomers. The polymerizable monomers comprise functional groups which do not take part in radical polymerization, under conditions where thermally initiated graft copolymerization of the monomers takes place, to form polymer chains on the surface of the support. If the polymer chains do not already comprise primary amino-functional groups, the method further comprises transforming the functional groups in the polymer chains into groups comprising primary amino-functional groups.
    Type: Application
    Filed: October 20, 2010
    Publication date: August 16, 2012
    Applicant: GAMBRO LUNDIA AB
    Inventors: Ralf Flieg, Manuela Klotz, Markus Storr, Martin Rempfer, Wolfgang Freudemann, Cornelia Winz, Bernd Krause, Markus Hornung
  • Patent number: 8236153
    Abstract: An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1, 4-naphthoquinone (ACNQ) as the electron acceptor compound.
    Type: Grant
    Filed: February 27, 2009
    Date of Patent: August 7, 2012
    Assignee: Sony Corporation
    Inventors: Atsushi Sato, Tokuji Ikeda, Kenji Kano
  • Publication number: 20120196345
    Abstract: The present invention generally relates to modified substrates such as membranes for use in bioartificial organs, such as bioartificial kidneys, and other applications. Certain aspects are generally directed to a membrane or other substrate modified to facilitate the attachment of cells. In one set of embodiments, the substrate or membrane may be at least partially coated with an adhesive such as 3,4-dihydroxy-L-phenylalanine (DOPA), poly(dopamine), or other adhesive comprising a molecule having a catechol moiety, for example on one side of the membrane or substrate. On at least a portion of the adhesive coated portion of the substrate, a protein may be coated, such as an extracellular matrix protein (for example, a collagen), to which cells such as primary human renal proximal tubule cells may be adhered. Surprisingly, such a dual coating may be used to promote the attachment of such cells to a membrane or other substrate that otherwise may not promote cell adhesion.
    Type: Application
    Filed: February 2, 2011
    Publication date: August 2, 2012
    Applicant: Agency for Science, Technology and Research
    Inventors: Daniele Zink, Ming Ni, Jackie Y. Ying
  • Publication number: 20120094354
    Abstract: A biochip and a method for manufacturing the same are provided. The biochip can increase a surface area on which biomaterials can be immobilized and improve efficiency of reaction. The biochip includes: a substrate; a coating layer formed on the substrate; and a nanoparticle immobilized on the substrate by the coating layer, a material to be bound to a target material being attached on a surface of the nanoparticle.
    Type: Application
    Filed: October 4, 2011
    Publication date: April 19, 2012
    Applicant: Electronics and Telecommunications Research Institute
    Inventor: Nae Rym Lee
  • Patent number: RE43979
    Abstract: The invention relates to a method for detecting specific target-cells in a simple and time saving way, using paramagnetic particles, antibodies recognizing the Fc portions of target-cell associating antibodies and target-cell associating antibodies directed to specific antigen determinants in the target-cell membranes. Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotincomplexes or certain enzymes allowing visualization, with dramatically increase the specificity of the method. The method can further be used for isolation of the target-cells by magnetic field application and kit for performing the method according to the invention is described.
    Type: Grant
    Filed: February 5, 2003
    Date of Patent: February 5, 2013
    Assignee: Abbott Laboratories
    Inventors: Oystein Fodstad, Gunnar Kvalheim